Galen M Pieper

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. ncbi request reprint Inhibition of nitrosylation, nitration, lymphocyte proliferation, and gene expression in acute and delayed cardiac allograft rejection by an orally active dithiocarbamate
    Galen M Pieper
    Department of Surgery Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Cardiovasc Pharmacol 43:522-30. 2004
  2. ncbi request reprint A dithiocarbamate analogue decreases intraislet cell infiltration and the incidence of diabetes mellitus in the genetic diabetes-prone BB rat
    Galen M Pieper
    Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, Froedtert Memorial Hospital, Milwaukee, Wisconsin 53226, USA
    Pancreas 28:e16-25. 2004
  3. ncbi request reprint Variable efficacy of N6-(1-iminoethyl)-L-lysine in acute cardiac transplant rejection
    Galen M Pieper
    Division of Transplant Surgery, Department of Surgery, Cardiovascular Center, Medical College of Wisconsin, Milwaukee 53226, USA
    Am J Physiol Heart Circ Physiol 286:H525-34. 2004
  4. ncbi request reprint Spatial distribution and temporal onset of NF-kB activation and inducible nitric oxide synthase within pancreatic islets in the pre-diabetic stage of genetic, diabetic-prone BB rats: attenuation by drug intervention decreases inflammatory cell infiltratio
    C M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, Froedtert Memorial Hospital, West Wisconsin Avenue, Milwaukee, WI 53226, USA
    Inflamm Res 53:22-30. 2004
  5. pmc Reactive oxygen and reactive nitrogen as signaling molecules for caspase 3 activation in acute cardiac transplant rejection
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Antioxid Redox Signal 10:1031-40. 2008
  6. ncbi request reprint Treatment with {alpha}-phenyl-N-tert-butylnitrone, a free radical-trapping agent, abrogates inflammatory cytokine gene expression during alloimmune activation in rat cardiac allografts
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 312:774-9. 2005
  7. ncbi request reprint Protective mechanisms of a metalloporphyrinic peroxynitrite decomposition catalyst, WW85, in rat cardiac transplants
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, USA
    J Pharmacol Exp Ther 314:53-60. 2005
  8. pmc Sepiapterin decreases acute rejection and apoptosis in cardiac transplants independently of changes in nitric oxide and inducible nitric-oxide synthase dimerization
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Ave, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 329:890-9. 2009
  9. pmc Nitric oxide formation in acutely rejecting cardiac allografts correlates with GTP cyclohydrolase I activity
    Galen M Pieper
    Division of Transplant Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, USA
    Biochem J 391:541-7. 2005
  10. ncbi request reprint Chronic or delayed treatment with an oral dithiocarbamate analog decreases glycation and protects diabetic arteries
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Eur J Pharmacol 472:127-34. 2003

Detail Information

Publications35

  1. ncbi request reprint Inhibition of nitrosylation, nitration, lymphocyte proliferation, and gene expression in acute and delayed cardiac allograft rejection by an orally active dithiocarbamate
    Galen M Pieper
    Department of Surgery Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Cardiovasc Pharmacol 43:522-30. 2004
    ..These studies suggest that NOX-700 is protective in cardiac rejection, in part, by scavenging of NO and by limiting lymphocyte activation infiltration...
  2. ncbi request reprint A dithiocarbamate analogue decreases intraislet cell infiltration and the incidence of diabetes mellitus in the genetic diabetes-prone BB rat
    Galen M Pieper
    Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, Froedtert Memorial Hospital, Milwaukee, Wisconsin 53226, USA
    Pancreas 28:e16-25. 2004
    ..These studies demonstrate the potential therapeutic efficacy of dithiocarbamates to oppose the development of autoimmune insulin-dependent diabetes mellitus by limiting inflammatory cell activation/infiltration...
  3. ncbi request reprint Variable efficacy of N6-(1-iminoethyl)-L-lysine in acute cardiac transplant rejection
    Galen M Pieper
    Division of Transplant Surgery, Department of Surgery, Cardiovascular Center, Medical College of Wisconsin, Milwaukee 53226, USA
    Am J Physiol Heart Circ Physiol 286:H525-34. 2004
    ..These studies have important implications in understanding the dual role of NO in acute rejection and help to reconcile discrepancies in the literature...
  4. ncbi request reprint Spatial distribution and temporal onset of NF-kB activation and inducible nitric oxide synthase within pancreatic islets in the pre-diabetic stage of genetic, diabetic-prone BB rats: attenuation by drug intervention decreases inflammatory cell infiltratio
    C M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, Froedtert Memorial Hospital, West Wisconsin Avenue, Milwaukee, WI 53226, USA
    Inflamm Res 53:22-30. 2004
    ..To document in vivo immunolocalization and activation of nuclear factor-kappaB (NF-kappaB) and inducible nitric oxide synthase (iNOS) expression in prediabetic stages of diabetes mellitus...
  5. pmc Reactive oxygen and reactive nitrogen as signaling molecules for caspase 3 activation in acute cardiac transplant rejection
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Antioxid Redox Signal 10:1031-40. 2008
    ..These studies indicate that both superoxide and NO (precursors of peroxynitrite formation) play a significant role in caspase 3 activation in cardiac allograft rejection...
  6. ncbi request reprint Treatment with {alpha}-phenyl-N-tert-butylnitrone, a free radical-trapping agent, abrogates inflammatory cytokine gene expression during alloimmune activation in rat cardiac allografts
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 312:774-9. 2005
    ..Thus, PBN retains significant anti-inflammatory properties through its action to down-regulate cytokine gene expression that contribute to protection against acute alloimmune activation in cardiac allografts...
  7. ncbi request reprint Protective mechanisms of a metalloporphyrinic peroxynitrite decomposition catalyst, WW85, in rat cardiac transplants
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, USA
    J Pharmacol Exp Ther 314:53-60. 2005
    ..Rather, effects seem to be downstream on decreased protein nitration, decreased lipid peroxidation, and decreased PARP activation...
  8. pmc Sepiapterin decreases acute rejection and apoptosis in cardiac transplants independently of changes in nitric oxide and inducible nitric-oxide synthase dimerization
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Ave, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 329:890-9. 2009
    ....
  9. pmc Nitric oxide formation in acutely rejecting cardiac allografts correlates with GTP cyclohydrolase I activity
    Galen M Pieper
    Division of Transplant Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, USA
    Biochem J 391:541-7. 2005
    ..These findings provide insight into a potential pathway regulating NO bioactivity in graft rejection. Such knowledge may potentially assist in the design of newer strategies to prevent acute graft rejection...
  10. ncbi request reprint Chronic or delayed treatment with an oral dithiocarbamate analog decreases glycation and protects diabetic arteries
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Eur J Pharmacol 472:127-34. 2003
    ..Thus, vascular protection by NOX-700 is believed to be mediated, in part, by an antioxidant mechanism and decreased protein glycation...
  11. ncbi request reprint Mechanisms of the protective action of diethyldithiocarbamate-iron complex on acute cardiac allograft rejection
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 W Wisconsin Avenue, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 284:H1542-51. 2003
    ..Gene expression for the proinflammatory cytokine interferon-gamma was also decreased by DETC-Fe. Thus DETC-Fe limits NF-kappaB-dependent gene expression and possesses significant immunosuppressive properties...
  12. pmc Non-heme iron protein: a potential target of nitric oxide in acute cardiac allograft rejection
    Galen M Pieper
    Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, WI 53226, USA
    Proc Natl Acad Sci U S A 100:3125-30. 2003
    ..This report shows targeting of an important non-heme Fe-S cluster protein in acute solid organ transplant rejection...
  13. ncbi request reprint Reduction of myocardial nitrosyl complex formation by a nitric oxide scavenger prolongs cardiac allograft survival
    G M Pieper
    Department of Surgery, Biophysics Research Institute, Medical College of Wisconsin, Milwaukee 53226, USA
    J Cardiovasc Pharmacol 35:114-20. 2000
    ..These studies support a role of NO in cardiac allograft rejection. Furthermore, these studies indicate a potential therapeutic value of NO scavengers in preventing organ rejection...
  14. ncbi request reprint Decreased opioid-induced antinociception but unaltered G-protein activation in the genetic-diabetic NOD mouse
    G M Pieper
    Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, Froedtert Memorial Lutheran Hospital, 9200 West Wisconsin Avenue, 53226, Milwaukee, WI, USA
    Eur J Pharmacol 401:375-9. 2000
    ..This suggests that a G-protein defect in signaling cannot account for the hyporesponsiveness of antinociception in this genetic model of IDDM...
  15. pmc The complex role of iNOS in acutely rejecting cardiac transplants
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 44:1536-52. 2008
    ..Finally, the concept of a dual role for NO and its downstream product, peroxynitrite, in rejection vs immune regulation is discussed...
  16. ncbi request reprint Antioxidant treatment inhibits activation of myocardial nuclear factor kappa B and inhibits nitrosylation of myocardial heme protein in cardiac transplant rejection
    G M Pieper
    Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Antioxid Redox Signal 3:81-8. 2001
    ..These studies provide evidence that reactive oxygen plays a significant role in signal transduction for activation via the transcription factor, NF-kappa B, thereby modulating distal actions and consequences of iNOS-derived nitric oxide...
  17. ncbi request reprint A ruthenium (III) polyaminocarboxylate complex, a novel nitric oxide scavenger, enhances graft survival and decreases nitrosylated heme protein in models of acute and delayed cardiac transplant rejection
    Galen M Pieper
    Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Cardiovasc Pharmacol 39:441-8. 2002
    ....
  18. ncbi request reprint Prolonging organ allograft survival: potential role of nitric oxide scavengers
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, WI 53226, USA
    BioDrugs 16:37-45. 2002
    ....
  19. ncbi request reprint Vascular protective actions of a nitric oxide aspirin analog in both in vitro and in vivo models of diabetes mellitus
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 32:1143-56. 2002
    ....
  20. pmc Speckle-tracking 2-dimensional strain echocardiography: a new noninvasive imaging tool to evaluate acute rejection in cardiac transplantation
    Galen M Pieper
    Department of Surgery Transplant Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Heart Lung Transplant 29:1039-46. 2010
    ..We aim to determine if 2DSE-derived functional indices can detect cardiac transplant rejection...
  21. ncbi request reprint Transfection of human endothelial cells with HIV-1 tat gene activates NF-kappa B and enhances monocyte adhesion
    Galen M Pieper
    Division of Transplant Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee 53226, USA
    Am J Physiol Heart Circ Physiol 283:H2315-21. 2002
    ..Furthermore, inhibition studies using aspirin suggest that pcTat-stimulated NF-kappaB activation and monocyte adhesion occur via a redox-sensitive mechanism...
  22. ncbi request reprint Hierarchical change in antioxidant enzyme gene expression and activity in acute cardiac rejection: role of inducible nitric oxide synthase
    Vani Nilakantan
    Department of Surgery, Division of Transplant Surgery, Milwaukee, WI 53226, USA
    Mol Cell Biochem 270:39-47. 2005
    ..This suggests a differential role for iNOS in regulating post-translational modification of individual antioxidant enzymes in acute cardiac transplantation...
  23. ncbi request reprint Post-translational modification of manganese superoxide dismutase in acutely rejecting cardiac transplants: role of inducible nitric oxide synthase
    Vani Nilakantan
    Division of Transplant Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Heart Lung Transplant 24:1591-9. 2005
    ..In this study, we examined the effect of inducible nitric oxide synthase (iNOS) on MnSOD expression, activity and nitration in acutely rejecting cardiac transplants...
  24. pmc Cardiac myocyte-specific overexpression of human GTP cyclohydrolase I protects against acute cardiac allograft rejection
    Irina A Ionova
    Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Ave, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 299:H88-96. 2010
    ..The mechanism of the decreased rejection appears related to decreased T cell proliferation and modulation of immune function by higher expression of genes involved in hematopoietic/stromal cell development and recruitment...
  25. ncbi request reprint Beneficial effect of alpha-tocopheryl succinate in rat cardiac transplants
    Thanh K Nguyen
    Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Heart Lung Transplant 25:707-15. 2006
    ..In this study we examined the effects of a novel vitamin E analog, alpha-tocopheryl polyethylene glycol-100 succinate (alpha-TPGS), and low-dose cyclosporine (CsA) in the treatment of acute and delayed cardiac rejection...
  26. pmc Deficient BH4 production via de novo and salvage pathways regulates NO responses to cytokines in adult cardiac myocytes
    Irina A Ionova
    Department of Surgery Transplant Surgery, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 295:H2178-87. 2008
    ....
  27. ncbi request reprint Antagonizing reactive oxygen by treatment with a manganese (III) metalloporphyrin-based superoxide dismutase mimetic in cardiac transplants
    Vani Nilakantan
    Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, Wis 53226, USA
    J Thorac Cardiovasc Surg 131:898-906. 2006
    ....
  28. pmc Cytokines and lipopolysaccharides induce inducible nitric oxide synthase but not enzyme activity in adult rat cardiomyocytes
    Jeannette Vasquez-Vivar
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 45:994-1001. 2008
    ..This finding adds a new and unexpected layer of complexity to our understanding of the responses of the adult heart to inflammation...
  29. pmc Cardiac-specific overexpression of GTP cyclohydrolase 1 restores ischaemic preconditioning during hyperglycaemia
    Zhi Dong Ge
    Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Cardiovasc Res 91:340-9. 2011
    ....
  30. ncbi request reprint Favorable balance of anti-oxidant/pro-oxidant systems and ablated oxidative stress in Brown Norway rats in renal ischemia-reperfusion injury
    Vani Nilakantan
    Division of Transplant Surgery, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Mol Cell Biochem 304:1-11. 2007
    ..01) following IR. Collectively these results show that the resistance of the BN rat to renal IR injury is associated with a favorable balance of oxidant production vs. oxidant removal...
  31. ncbi request reprint Recombinant p21 protein inhibits lymphocyte proliferation and transcription factors
    Ashwani K Khanna
    Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Immunol 174:7610-7. 2005
    ....
  32. ncbi request reprint Anti-transforming growth factor antibody at low but not high doses limits cyclosporine-mediated nephrotoxicity without altering rat cardiac allograft survival: potential of therapeutic applications
    Ashwani K Khanna
    Division of Nephrology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Circulation 110:3822-9. 2004
    ..Transforming growth factor-beta (TGF-beta) is one of the most potent mediators of the fibrogenic effects of cyclosporine...
  33. ncbi request reprint Altered meiotic regulation in oocytes from diabetic mice
    Shannondoah A Colton
    Biology Department, Marquette University, Milwaukee, WI 53233, USA
    Biol Reprod 67:220-31. 2002
    ....
  34. ncbi request reprint Differential response of postischemic diabetic myocardium to a thromboxane-mimetic
    G M Pieper
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee 53226
    Eicosanoids 3:127-33. 1990
    ....
  35. ncbi request reprint NADPH oxidase subunits (NOX-1, p22phox, Rac-1) and tacrolimus-induced nephrotoxicity in a rat renal transplant model
    Ashwani K Khanna
    Division of Cardiology, University of Maryland, 22 S Greene Street S 3B08, Baltimore, MD 21201, USA
    Nephrol Dial Transplant 22:376-85. 2007
    ..We examined whether anti-TGF-beta antibody limits nephrotoxicity produced by tacrolimus (TAC) and whether this altered genes that regulate oxidative stress...

Research Grants8

  1. MOLECULAR TARGETS OF NITRIC OXIDE IN CARDIAC TRANSPLANTS
    GALEN PIEPER; Fiscal Year: 2004
    ..These studies will provide a novel mechanism to explain the pathogenesis of dyskinesis and graft failure during cardiac transplant rejection. ..
  2. iNOS Posttranslational Regulation in Cardiac Rejection
    GALEN PIEPER; Fiscal Year: 2007
    ..A better understanding of this molecular process may lead to better strategies to prevent injury cardiac cells in these patients. ..
  3. iNOS Posttranslational Regulation in Cardiac Rejection
    GALEN PIEPER; Fiscal Year: 2009
    ..A better understanding of this molecular process may lead to better strategies to prevent injury cardiac cells in these patients. ..