Research Topics
Species | Makio OgawaSummaryAffiliation: Medical University of South Carolina Country: USA Publications
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Publications
Hematopoietic stem cells are pluripotent and not just "hematopoietic"Makio Ogawa
Department of Pathology and Laboratory Medicine, Ralph H Johnson VAMC, USA Electronic address
Blood Cells Mol Dis 51:3-8. 2013..Also discussed are some biological and experimental issues that need to be considered in the future investigation of HSC plasticity...
Changing phenotypes of hematopoietic stem cellsMakio Ogawa
Ralph H. Johnson VA Medical Center and Department of Medicine, Medical University of South Carolina, Charleston, SC 29401-5799, USA
Exp Hematol 30:3-6. 2002- Hematopoietic origin of fibroblasts/myofibroblasts: Its pathophysiologic implicationsMakio Ogawa
Department of Veterans Affairs Medical Center, Charleston, SC, USA
Blood 108:2893-6. 2006..This brief note summarizes these findings and discusses clinical and experimental perspectives generated by this newly identified differentiation pathway of HSCs... - Stochastic model revisitedM Ogawa
Department of Veterans Affairs Medical Center, Charleston, South Carolina, USA
Int J Hematol 69:2-5. 1999..This review summarizes some of the pertinent literature and discusses the cellular and molecular mechanisms of stem cell differentiation... - CD34 expression by murine hematopoietic stem cells. Developmental changes and kinetic alterationsM Ogawa
Departments of Medicine and Pediatrics, Medical University of South Carolina, USA
Ann N Y Acad Sci 938:139-45. 2001..An estimated 20% of the adult stem cells expressed CD34. These observations provide insight into the current controversy regarding CD34 expression by adult hematopoietic stem cells... - Humoral regulation of hematopoietic stem cellsM Ogawa
Department of Veterans Affairs Medical Center, Charleston, South Carolina 29401 5799, USA
Ann N Y Acad Sci 872:17-23; discussion 23-4. 1999..Surprisingly, IL-3 and IL-1 proved to have profound negative effects on hematopoietic stem cells. Studies of human hematopoietic stem cells are now necessary... 
Hematopoietic stem cell origin of connective tissuesMakio Ogawa
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC29401 5799, USA
Exp Hematol 38:540-7. 2010..This review summarizes the experimental evidence for this new paradigm and discusses its translational implications...- Hematopoietic stem cell origin of mesenchymal cells: opportunity for novel therapeutic approachesMakio Ogawa
Department of Pathology and Laboratory Medicine, VA Medical Center, Medical University of South Carolina, 109 Bee Street, Charleston, SC 29412, USA
Int J Hematol 91:353-9. 2010..The concept of the HSC origin of mesenchymal cells will have many immediate and long-term impacts on the therapies of diseases and injuries of the connective tissues... - Transplanted human cord blood cells generate amylase-producing pancreatic acinar cells in engrafted miceHitoshi Minamiguchi
Department of Veterans Affairs Medical Center and Department of Medicine, Medical University of South Carolina, Charleston, SC, USA
Pancreas 36:e30-5. 2008..Based on this developmental proximity between liver and pancreatic acinar cells, we examined whether human cord blood (CB) cells can generate pancreatic cells in vivo using a murine xenograft model... - Hematopoietic origins of fibroblasts: II. In vitro studies of fibroblasts, CFU-F, and fibrocytesYasuhiro Ebihara
Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, 29401-5799, USA
Exp Hematol 34:219-29. 2006..CONCLUSION: We conclude that a population of fibroblasts and their precursors are derived from HSCs... - Contribution of bone marrow hematopoietic stem cells to adult mouse inner ear: mesenchymal cells and fibrocytesHainan Lang
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, South Carolina 29425, USA
J Comp Neurol 496:187-201. 2006..The results suggest that mesenchymal cells, including fibrocytes in the adult inner ear, may be derived continuously from HSCs... - Hematopoietic stem cell origin of human fibroblasts: cell culture studies of female recipients of gender-mismatched stem cell transplantation and patients with chronic myelogenous leukemiaKeisuke Shirai
Department of Medicine, Medical University of South Carolina, Charleston, SC, USA
Exp Hematol 37:1464-71. 2009.... - Hematopoietic origins of fibroblasts: I. In vivo studies of fibroblasts associated with solid tumorsAmanda C LaRue
Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, USA
Exp Hematol 34:208-18. 2006..The second population was a perivascular cell associated with the CD31(+) tumor blood vessels. CONCLUSION: These in vivo findings establish an HSC origin of fibroblasts... - An in vivo analysis of hematopoietic stem cell potential: hematopoietic origin of cardiac valve interstitial cellsRichard P Visconti
Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC 29425, USA
Circ Res 98:690-6. 2006..Together, these findings demonstrate HSC contribution to the adult valve fibroblast population... - Hematopoietic stem cell origin of adipocytesYasuhiko Sera
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC 29401, USA
Exp Hematol 37:1108-20, 1120.e1-4. 2009..We recently reported that fibroblasts/myofibroblasts in a number of tissues and organs are derived from hematopoietic stem cells (HSCs). In the present study, we tested the hypothesis that HSCs also give rise to adipocytes... - Thrombocytopenia in mice lacking the carboxy-terminal regulatory domain of the Ets transcription factor Fli1Omar Moussa
Department of Pathology and Laboratory Medicine, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA
Mol Cell Biol 30:5194-206. 2010.... - An efficient method for single hematopoietic stem cell engraftment in mice based on cell-cycle dormancy of hematopoietic stem cellsTakanori Abe
Department of Pathology and Laboratory Medicine, Medical University of South Carolina and Research Service, Department of Veterans Affairs Medical Center, Charleston, 29401 5799, USA
Exp Hematol 38:603-8. 2010..To develop an efficient method for single hematopoietic stem cell (HSC) transplantation for high-level hematopoietic engraftment... - Dysregulation of granulocyte, erythrocyte, and NK cell lineages in Fli-1 gene-targeted miceMasahiro Masuya
Department of Veterans Affairs Medical Center, Charleston, SC 29401 5799, USA
Blood 105:95-102. 2005..Production of chimeras by morula-stage embryo aggregation is an effective way to unravel cell-autonomous hematopoietic defects in gene-targeted mice... - Hematopoietic origin of glomerular mesangial cellsMasahiro Masuya
Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, SC, USA
Blood 101:2215-8. 2003..These data demonstrate that a single hematopoietic stem cell is capable of differentiating into glomerular mesangial cells and that the process does not involve cell fusion... - Transplanted human cord blood cells give rise to hepatocytes in engrafted miceFumihiko Ishikawa
Department of Veterans Affairs Medical Center, Charleston, South Carolina 29401, USA
Ann N Y Acad Sci 996:174-85. 2003..Human cord blood cells can give rise to hepatocytes in a xenogeneic transplantation model. This model will be useful to further characterize the cord blood progenitors of hepatocytes... - Enhancement of murine hematopoiesis by synergistic interactions between steel factor (ligand for c-kit), interleukin-11, and other early acting factors in cultureK Tsuji
Department of Medicine, Medical University of South Carolina, Charleston
Blood 79:2855-60. 1992..In contrast, SF is unique among the synergistic factors in that it interacts either with growth factors such as IL-3 or GM-CSF or with synergistic factors such as IL-6, IL-11, or G-CSF... - Hematopoietic stem cells give rise to osteo-chondrogenic cellsMeenal Mehrotra
Department of Veterans Affairs Medical Center, Ralph H Johnson VAMC, Medical University of South Carolina, Charleston, SC, USA
Blood Cells Mol Dis 50:41-9. 2013..Together, these findings strongly support the concept that HSCs generate bone cells and suggest therapeutic potentials of HSCs in fracture repair... - Amelioration of a mouse model of osteogenesis imperfecta with hematopoietic stem cell transplantation: microcomputed tomography studiesMeenal Mehrotra
Research Services, Department of Veterans Affairs Medical Center, Charleston, SC 29401 5799, USA
Exp Hematol 38:593-602. 2010..Homozygous OI murine mice (oim; B6C3Fe a/a-Col1a2(oim)/J) offer excellent recipients for transplantation of normal HSCs, because fast turnover of osteoprogenitors has been shown... - An assay for human hematopoietic stem cells based on transplantation into nonobese diabetic recombination activating gene-null perforin-null miceHitoshi Minamiguchi
Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, South Carolina 29401-5799, USA
Biol Blood Marrow Transplant 11:487-94. 2005..Accordingly, conditioned newborn NOD- Rag1 null Prf1 null mice provide a desirable model for an assay of long-term transplantable human HSCs... - Thrombopoietin, the ligand for the Mpl receptor, synergizes with steel factor and other early acting cytokines in supporting proliferation of primitive hematopoietic progenitors of miceH Ku
Department of Medicine, Medical University of South Carolina, Charleston, USA
Blood 87:4544-51. 1996..Based on these observations, we propose that ML can function as an early acting cytokine and stimulate the proliferation of cell cycle dormant progenitors by shortening their G0 period... - Expression of lineage markers by CD34+ hematopoietic stem cells of adult miceAkaru Ishida
Department of Veterans Affairs, Ralph H Johnson VA Medical Center, 109 Bee Street, Charleston, SC 29401 5799, USA
Exp Hematol 30:361-5. 2002..By using a murine transplantation model, we studied the relationship between CD34 expression and expression of CD4 and Mac-1 by hematopoietic stem cells of normal adult mice... - Soluble thrombopoietin receptor (Mpl) and granulocyte colony-stimulating factor receptor directly stimulate proliferation of primitive hematopoietic progenitors of mice in synergy with steel factor or the ligand for Flt3/Flk2H Ku
Department of Medicine, Medical University of South Carolina, Charleston, USA
Blood 88:4124-31. 1996..The direct effects of the soluble receptors appear to be mediated through interactions with their respective membrane-bound receptors expressed on the primitive hematopoietic progenitors... - Bone marrow origin of hematopoietic progenitors and stem cells in murine muscleH Kawada
Department of Veterans Affairs Medical Center, Charleston, SC, USA
Blood 98:2008-13. 2001..It is proposed that hematopoietic progenitors and stem cells in the muscle tissue are of BM origin but their transition from BM to muscle may be a slow process... - CD38 expression by hematopoietic stem cells of newborn and juvenile miceY Higuchi
Department of Veterans Affairs Medical Center and Department of Medicine, Medical University of South Carolina, Charleston, SC 29401 5799, USA
Leukemia 17:171-4. 2003..These observations may have implications for CD38 expression by HSCs from human umbilical cord blood and bone marrow of young children in steady state and under pathological conditions... - Hematopoietic progenitors and stem cells in murine muscleH Kawada
Department of Veterans Affairs Medical Center, 109 Bee Street, Medical University of South Carolina, Charleston, SC 29401, USA
Blood Cells Mol Dis 27:605-9. 2001..The hematopoietic progenitors and stem cells were present only in the Ly-5-positive fraction... - An assay for long-term engrafting human hematopoietic cells based on newborn NOD/SCID/beta2-microglobulin(null) miceFumihiko Ishikawa
Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, SC 29401-5799, USA
Exp Hematol 30:488-94. 2002..5 +/- 0.42% at 2 months after secondary transplantation. CONCLUSION: Our assay may provide a quantitative method for analysis of human hematopoietic stem cells... - Total body irradiation selectively induces murine hematopoietic stem cell senescenceYong Wang
Department of Pathology and Laboratory Medicine, MUSC, 165 Ashley Ave, Suite 309, Charleston, SC 29425, USA
Blood 107:358-66. 2006..This suggests that p21(Cip1/Waf1), p19(Arf), and p16(Ink4a) may play an important role in IR-induced senescence in HSCs... - Marker-assisted study of genetic background and gene-targeted locus modifiers in lymphopoietic phenotypesDemetri D Spyropoulos
Laboratory of Cancer Genomics, Hollings Cancer Center, Department of Cell Biology and Anatomy, Medical University of South Carolina, 86 Jonathan Lucas St, Charleston, SC 29425, USA
Anticancer Res 23:2015-26. 2003..Marker-assisted strategies for the identification of genetic modifiers, especially those linked to the targeted locus, will also be considered... - Engraftment and multilineage expression of human bone marrow CD34- cells in vivoE D Zanjani
Department of Veterans Affairs Medical Center, University of Nevada Reno 89520, USA
Ann N Y Acad Sci 872:220-31; discussion 231-2. 1999..Secondary transplantation and limiting dilution studies confirmed the presence in human CD34- fraction of HSC with in vivo long-term engraftment and multilineage differentiation potentials... - Reciprocal expression of CD38 and CD34 by adult murine hematopoietic stem cellsF Tajima
Departments of Medicine and Pediatrics, Medical University of South Carolina, Charleston, USA
Blood 97:2618-24. 2001..This reversible and reciprocal expression of CD38 and CD34 by murine stem cells may have implications for the phenotypes of human stem cells... - Human cord blood long-term engrafting cells are CD34+ CD38-F Ishikawa
Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, SC 29401, USA
Leukemia 17:960-4. 2003..These findings are consistent with the results from studies of murine stem cells and strongly indicate that the phenotype of human CB stem cells is CD34(+) CD38(-)... - Defective megakaryopoiesis and abnormal erythroid development in Fli-1 gene-targeted miceH Kawada
Department of Medicine, Hollings Cancer Center, Medical University of South Carolina, Charleston, USA
Int J Hematol 73:463-8. 2001..These results provide further evidence for the role of Fli-1 in the regulation of hematopoiesis and for c-mpl as a Fli-1 target gene... - Hemorrhage, impaired hematopoiesis, and lethality in mouse embryos carrying a targeted disruption of the Fli1 transcription factorD D Spyropoulos
Center for Molecular and Structural Biology, Medical University of South Carolina, USA
Mol Cell Biol 20:5643-52. 2000..Taken together, these results provide in vivo evidence for the role of Fli1 in the regulation of hematopoiesis and hemostasis... - Overexpression of Hoxc13 in differentiating keratinocytes results in downregulation of a novel hair keratin gene cluster and alopeciaA V Tkatchenko
Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA
Development 128:1547-58. 2001..Combined, these results provide a framework for understanding molecular mechanisms of Hoxc13 function in hair growth and development... - Reversible expression of CD34 by adult human bone marrow long-term engrafting hematopoietic stem cellsEsmail D Zanjani
Department of Animal Biotechnology, University of Nevada, Reno, Nev, USA
Exp Hematol 31:406-12. 2003.... - Hematopoietic origin of microglial and perivascular cells in brainDavid C Hess
Department of Neurology, Medical College of Georgia, Augusta, GA 30912, USA
Exp Neurol 186:134-44. 2004.... - Origin of fibroblast colony-forming unitsMakio Ogawa
Exp Hematol 35:1319-20. 2007
Research Grants
- Tissue Reconstituting Potential of Human Stem CellsMakio Ogawa; Fiscal Year: 2007..Specific Aim #3: To determine the developmental control of the tissue reconstituting potentials of the stem cells in neonatal and adult mouse xenografts. ..
- Transdifferentiation Potential of Bone Marrow Stem CellsMakio Ogawa; Fiscal Year: 2006..abstract_text> ..
- IDENTIFICATION AND PURIFICATION OF HUMAN STEM CELLSMakio Ogawa; Fiscal Year: 2001..NOD/SCID mouse model is more practical for limiting dilution studies. We already have exciting preliminary data regarding the use of myeloablated NOD/SCID newborn mice for quantitation of human engrafting cells. ..
- Hematopoietic Origin of Mesenchymal CellsMakio Ogawa; Fiscal Year: 2009..e. abilities to generate fat tissues, bones and cartilages. The knowledge gained from these studies will allow subsequent development of new, unique avenues of therapy for obesity and diseases of bones and joints. ..