Makio Ogawa

Summary

Affiliation: Medical University of South Carolina
Country: USA

Publications

  1. pmc Hematopoietic stem cells are pluripotent and not just "hematopoietic"
    Makio Ogawa
    Department of Pathology and Laboratory Medicine, Ralph H Johnson VAMC, USA
    Blood Cells Mol Dis 51:3-8. 2013
  2. ncbi request reprint Stochastic model revisited
    M Ogawa
    Department of Veterans Affairs Medical Center, Charleston, South Carolina, USA
    Int J Hematol 69:2-5. 1999
  3. ncbi request reprint Changing phenotypes of hematopoietic stem cells
    Makio Ogawa
    Ralph H Johnson VA Medical Center and Department of Medicine, Medical University of South Carolina, Charleston, SC 29401 5799, USA
    Exp Hematol 30:3-6. 2002
  4. ncbi request reprint Hematopoietic origin of fibroblasts/myofibroblasts: Its pathophysiologic implications
    Makio Ogawa
    Department of Veterans Affairs Medical Center, Charleston, SC, USA
    Blood 108:2893-6. 2006
  5. ncbi request reprint CD34 expression by murine hematopoietic stem cells. Developmental changes and kinetic alterations
    M Ogawa
    Departments of Medicine and Pediatrics, Medical University of South Carolina, USA
    Ann N Y Acad Sci 938:139-45. 2001
  6. ncbi request reprint Humoral regulation of hematopoietic stem cells
    M Ogawa
    Department of Veterans Affairs Medical Center, Charleston, South Carolina 29401 5799, USA
    Ann N Y Acad Sci 872:17-23; discussion 23-4. 1999
  7. ncbi request reprint Hematopoietic stem cell origin of connective tissues
    Makio Ogawa
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC29401 5799, USA
    Exp Hematol 38:540-7. 2010
  8. doi request reprint Hematopoietic stem cell origin of mesenchymal cells: opportunity for novel therapeutic approaches
    Makio Ogawa
    Department of Pathology and Laboratory Medicine, VA Medical Center, Medical University of South Carolina, 109 Bee Street, Charleston, SC 29412, USA
    Int J Hematol 91:353-9. 2010
  9. doi request reprint Transplanted human cord blood cells generate amylase-producing pancreatic acinar cells in engrafted mice
    Hitoshi Minamiguchi
    Department of Veterans Affairs Medical Center and Department of Medicine, Medical University of South Carolina, Charleston, SC, USA
    Pancreas 36:e30-5. 2008
  10. ncbi request reprint Hematopoietic origins of fibroblasts: II. In vitro studies of fibroblasts, CFU-F, and fibrocytes
    Yasuhiro Ebihara
    Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, 29401 5799, USA
    Exp Hematol 34:219-29. 2006

Collaborators

Detail Information

Publications42

  1. pmc Hematopoietic stem cells are pluripotent and not just "hematopoietic"
    Makio Ogawa
    Department of Pathology and Laboratory Medicine, Ralph H Johnson VAMC, USA
    Blood Cells Mol Dis 51:3-8. 2013
    ..Also discussed are some biological and experimental issues that need to be considered in the future investigation of HSC plasticity...
  2. ncbi request reprint Stochastic model revisited
    M Ogawa
    Department of Veterans Affairs Medical Center, Charleston, South Carolina, USA
    Int J Hematol 69:2-5. 1999
    ..This review summarizes some of the pertinent literature and discusses the cellular and molecular mechanisms of stem cell differentiation...
  3. ncbi request reprint Changing phenotypes of hematopoietic stem cells
    Makio Ogawa
    Ralph H Johnson VA Medical Center and Department of Medicine, Medical University of South Carolina, Charleston, SC 29401 5799, USA
    Exp Hematol 30:3-6. 2002
  4. ncbi request reprint Hematopoietic origin of fibroblasts/myofibroblasts: Its pathophysiologic implications
    Makio Ogawa
    Department of Veterans Affairs Medical Center, Charleston, SC, USA
    Blood 108:2893-6. 2006
    ..This brief note summarizes these findings and discusses clinical and experimental perspectives generated by this newly identified differentiation pathway of HSCs...
  5. ncbi request reprint CD34 expression by murine hematopoietic stem cells. Developmental changes and kinetic alterations
    M Ogawa
    Departments of Medicine and Pediatrics, Medical University of South Carolina, USA
    Ann N Y Acad Sci 938:139-45. 2001
    ..An estimated 20% of the adult stem cells expressed CD34. These observations provide insight into the current controversy regarding CD34 expression by adult hematopoietic stem cells...
  6. ncbi request reprint Humoral regulation of hematopoietic stem cells
    M Ogawa
    Department of Veterans Affairs Medical Center, Charleston, South Carolina 29401 5799, USA
    Ann N Y Acad Sci 872:17-23; discussion 23-4. 1999
    ..Surprisingly, IL-3 and IL-1 proved to have profound negative effects on hematopoietic stem cells. Studies of human hematopoietic stem cells are now necessary...
  7. ncbi request reprint Hematopoietic stem cell origin of connective tissues
    Makio Ogawa
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC29401 5799, USA
    Exp Hematol 38:540-7. 2010
    ..This review summarizes the experimental evidence for this new paradigm and discusses its translational implications...
  8. doi request reprint Hematopoietic stem cell origin of mesenchymal cells: opportunity for novel therapeutic approaches
    Makio Ogawa
    Department of Pathology and Laboratory Medicine, VA Medical Center, Medical University of South Carolina, 109 Bee Street, Charleston, SC 29412, USA
    Int J Hematol 91:353-9. 2010
    ..The concept of the HSC origin of mesenchymal cells will have many immediate and long-term impacts on the therapies of diseases and injuries of the connective tissues...
  9. doi request reprint Transplanted human cord blood cells generate amylase-producing pancreatic acinar cells in engrafted mice
    Hitoshi Minamiguchi
    Department of Veterans Affairs Medical Center and Department of Medicine, Medical University of South Carolina, Charleston, SC, USA
    Pancreas 36:e30-5. 2008
    ..Based on this developmental proximity between liver and pancreatic acinar cells, we examined whether human cord blood (CB) cells can generate pancreatic cells in vivo using a murine xenograft model...
  10. ncbi request reprint Hematopoietic origins of fibroblasts: II. In vitro studies of fibroblasts, CFU-F, and fibrocytes
    Yasuhiro Ebihara
    Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, 29401 5799, USA
    Exp Hematol 34:219-29. 2006
    ..Because myofibroblasts are thought to be an activated form of fibroblasts, we tested the hypothesis that fibroblasts are derived from HSCs...
  11. pmc Contribution of bone marrow hematopoietic stem cells to adult mouse inner ear: mesenchymal cells and fibrocytes
    Hainan Lang
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Comp Neurol 496:187-201. 2006
    ..The results suggest that mesenchymal cells, including fibrocytes in the adult inner ear, may be derived continuously from HSCs...
  12. pmc Hematopoietic stem cell origin of human fibroblasts: cell culture studies of female recipients of gender-mismatched stem cell transplantation and patients with chronic myelogenous leukemia
    Keisuke Shirai
    Department of Medicine, Medical University of South Carolina, Charleston, SC, USA
    Exp Hematol 37:1464-71. 2009
    ....
  13. ncbi request reprint Hematopoietic origins of fibroblasts: I. In vivo studies of fibroblasts associated with solid tumors
    Amanda C LaRue
    Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, USA
    Exp Hematol 34:208-18. 2006
    ..However, the bone marrow cell(s) that gives rise to fibroblasts has not yet been identified. In the present study, we tested the hypothesis that tissue fibroblasts are derived from hematopoietic stem cells (HSCs) in vivo...
  14. ncbi request reprint An in vivo analysis of hematopoietic stem cell potential: hematopoietic origin of cardiac valve interstitial cells
    Richard P Visconti
    Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC 29425, USA
    Circ Res 98:690-6. 2006
    ..Together, these findings demonstrate HSC contribution to the adult valve fibroblast population...
  15. pmc Hematopoietic stem cell origin of adipocytes
    Yasuhiko Sera
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC 29401, USA
    Exp Hematol 37:1108-20, 1120.e1-4. 2009
    ..We recently reported that fibroblasts/myofibroblasts in a number of tissues and organs are derived from hematopoietic stem cells (HSCs). In the present study, we tested the hypothesis that HSCs also give rise to adipocytes...
  16. pmc Thrombocytopenia in mice lacking the carboxy-terminal regulatory domain of the Ets transcription factor Fli1
    Omar Moussa
    Department of Pathology and Laboratory Medicine, Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Cell Biol 30:5194-206. 2010
    ....
  17. pmc An efficient method for single hematopoietic stem cell engraftment in mice based on cell-cycle dormancy of hematopoietic stem cells
    Takanori Abe
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina and Research Service, Department of Veterans Affairs Medical Center, Charleston, 29401 5799, USA
    Exp Hematol 38:603-8. 2010
    ..To develop an efficient method for single hematopoietic stem cell (HSC) transplantation for high-level hematopoietic engraftment...
  18. ncbi request reprint Dysregulation of granulocyte, erythrocyte, and NK cell lineages in Fli-1 gene-targeted mice
    Masahiro Masuya
    Department of Veterans Affairs Medical Center, Charleston, SC 29401 5799, USA
    Blood 105:95-102. 2005
    ..Production of chimeras by morula-stage embryo aggregation is an effective way to unravel cell-autonomous hematopoietic defects in gene-targeted mice...
  19. ncbi request reprint Hematopoietic origin of glomerular mesangial cells
    Masahiro Masuya
    Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, SC, USA
    Blood 101:2215-8. 2003
    ..These data demonstrate that a single hematopoietic stem cell is capable of differentiating into glomerular mesangial cells and that the process does not involve cell fusion...
  20. ncbi request reprint Enhancement of murine hematopoiesis by synergistic interactions between steel factor (ligand for c-kit), interleukin-11, and other early acting factors in culture
    K Tsuji
    Department of Medicine, Medical University of South Carolina, Charleston
    Blood 79:2855-60. 1992
    ..In contrast, SF is unique among the synergistic factors in that it interacts either with growth factors such as IL-3 or GM-CSF or with synergistic factors such as IL-6, IL-11, or G-CSF...
  21. ncbi request reprint An assay for human hematopoietic stem cells based on transplantation into nonobese diabetic recombination activating gene-null perforin-null mice
    Hitoshi Minamiguchi
    Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, South Carolina 29401 5799, USA
    Biol Blood Marrow Transplant 11:487-94. 2005
    ..Accordingly, conditioned newborn NOD- Rag1 null Prf1 null mice provide a desirable model for an assay of long-term transplantable human HSCs...
  22. pmc Hematopoietic stem cells give rise to osteo-chondrogenic cells
    Meenal Mehrotra
    Department of Veterans Affairs Medical Center, Ralph H Johnson VAMC, Medical University of South Carolina, Charleston, SC, USA
    Blood Cells Mol Dis 50:41-9. 2013
    ..Together, these findings strongly support the concept that HSCs generate bone cells and suggest therapeutic potentials of HSCs in fracture repair...
  23. ncbi request reprint Transplanted human cord blood cells give rise to hepatocytes in engrafted mice
    Fumihiko Ishikawa
    Department of Veterans Affairs Medical Center, Charleston, South Carolina 29401, USA
    Ann N Y Acad Sci 996:174-85. 2003
    ..Human cord blood cells can give rise to hepatocytes in a xenogeneic transplantation model. This model will be useful to further characterize the cord blood progenitors of hepatocytes...
  24. pmc Amelioration of a mouse model of osteogenesis imperfecta with hematopoietic stem cell transplantation: microcomputed tomography studies
    Meenal Mehrotra
    Research Services, Department of Veterans Affairs Medical Center, Charleston, SC 29401 5799, USA
    Exp Hematol 38:593-602. 2010
    ..Homozygous OI murine mice (oim; B6C3Fe a/a-Col1a2(oim)/J) offer excellent recipients for transplantation of normal HSCs, because fast turnover of osteoprogenitors has been shown...
  25. ncbi request reprint Thrombopoietin, the ligand for the Mpl receptor, synergizes with steel factor and other early acting cytokines in supporting proliferation of primitive hematopoietic progenitors of mice
    H Ku
    Department of Medicine, Medical University of South Carolina, Charleston, USA
    Blood 87:4544-51. 1996
    ..Based on these observations, we propose that ML can function as an early acting cytokine and stimulate the proliferation of cell cycle dormant progenitors by shortening their G0 period...
  26. ncbi request reprint Expression of lineage markers by CD34+ hematopoietic stem cells of adult mice
    Akaru Ishida
    Department of Veterans Affairs, Ralph H Johnson VA Medical Center, 109 Bee Street, Charleston, SC 29401 5799, USA
    Exp Hematol 30:361-5. 2002
    ..By using a murine transplantation model, we studied the relationship between CD34 expression and expression of CD4 and Mac-1 by hematopoietic stem cells of normal adult mice...
  27. ncbi request reprint Soluble thrombopoietin receptor (Mpl) and granulocyte colony-stimulating factor receptor directly stimulate proliferation of primitive hematopoietic progenitors of mice in synergy with steel factor or the ligand for Flt3/Flk2
    H Ku
    Department of Medicine, Medical University of South Carolina, Charleston, USA
    Blood 88:4124-31. 1996
    ..The direct effects of the soluble receptors appear to be mediated through interactions with their respective membrane-bound receptors expressed on the primitive hematopoietic progenitors...
  28. ncbi request reprint Bone marrow origin of hematopoietic progenitors and stem cells in murine muscle
    H Kawada
    Department of Veterans Affairs Medical Center, Charleston, SC, USA
    Blood 98:2008-13. 2001
    ..It is proposed that hematopoietic progenitors and stem cells in the muscle tissue are of BM origin but their transition from BM to muscle may be a slow process...
  29. ncbi request reprint CD38 expression by hematopoietic stem cells of newborn and juvenile mice
    Y Higuchi
    Department of Veterans Affairs Medical Center and Department of Medicine, Medical University of South Carolina, Charleston, SC 29401 5799, USA
    Leukemia 17:171-4. 2003
    ..These observations may have implications for CD38 expression by HSCs from human umbilical cord blood and bone marrow of young children in steady state and under pathological conditions...
  30. ncbi request reprint Hematopoietic progenitors and stem cells in murine muscle
    H Kawada
    Department of Veterans Affairs Medical Center, 109 Bee Street, Medical University of South Carolina, Charleston, SC 29401, USA
    Blood Cells Mol Dis 27:605-9. 2001
    ..The hematopoietic progenitors and stem cells were present only in the Ly-5-positive fraction...
  31. ncbi request reprint An assay for long-term engrafting human hematopoietic cells based on newborn NOD/SCID/beta2-microglobulin(null) mice
    Fumihiko Ishikawa
    Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, SC 29401 5799, USA
    Exp Hematol 30:488-94. 2002
    ....
  32. ncbi request reprint Marker-assisted study of genetic background and gene-targeted locus modifiers in lymphopoietic phenotypes
    Demetri D Spyropoulos
    Laboratory of Cancer Genomics, Hollings Cancer Center, Department of Cell Biology and Anatomy, Medical University of South Carolina, 86 Jonathan Lucas St, Charleston, SC 29425, USA
    Anticancer Res 23:2015-26. 2003
    ..Marker-assisted strategies for the identification of genetic modifiers, especially those linked to the targeted locus, will also be considered...
  33. pmc Total body irradiation selectively induces murine hematopoietic stem cell senescence
    Yong Wang
    Department of Pathology and Laboratory Medicine, MUSC, 165 Ashley Ave, Suite 309, Charleston, SC 29425, USA
    Blood 107:358-66. 2006
    ..This suggests that p21(Cip1/Waf1), p19(Arf), and p16(Ink4a) may play an important role in IR-induced senescence in HSCs...
  34. ncbi request reprint Engraftment and multilineage expression of human bone marrow CD34- cells in vivo
    E D Zanjani
    Department of Veterans Affairs Medical Center, University of Nevada Reno 89520, USA
    Ann N Y Acad Sci 872:220-31; discussion 231-2. 1999
    ..Secondary transplantation and limiting dilution studies confirmed the presence in human CD34- fraction of HSC with in vivo long-term engraftment and multilineage differentiation potentials...
  35. ncbi request reprint Reciprocal expression of CD38 and CD34 by adult murine hematopoietic stem cells
    F Tajima
    Departments of Medicine and Pediatrics, Medical University of South Carolina, Charleston, USA
    Blood 97:2618-24. 2001
    ..This reversible and reciprocal expression of CD38 and CD34 by murine stem cells may have implications for the phenotypes of human stem cells...
  36. ncbi request reprint Human cord blood long-term engrafting cells are CD34+ CD38-
    F Ishikawa
    Department of Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, SC 29401, USA
    Leukemia 17:960-4. 2003
    ..These findings are consistent with the results from studies of murine stem cells and strongly indicate that the phenotype of human CB stem cells is CD34(+) CD38(-)...
  37. pmc Hemorrhage, impaired hematopoiesis, and lethality in mouse embryos carrying a targeted disruption of the Fli1 transcription factor
    D D Spyropoulos
    Center for Molecular and Structural Biology, Medical University of South Carolina, USA
    Mol Cell Biol 20:5643-52. 2000
    ..Taken together, these results provide in vivo evidence for the role of Fli1 in the regulation of hematopoiesis and hemostasis...
  38. ncbi request reprint Overexpression of Hoxc13 in differentiating keratinocytes results in downregulation of a novel hair keratin gene cluster and alopecia
    A V Tkatchenko
    Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA
    Development 128:1547-58. 2001
    ..Combined, these results provide a framework for understanding molecular mechanisms of Hoxc13 function in hair growth and development...
  39. ncbi request reprint Defective megakaryopoiesis and abnormal erythroid development in Fli-1 gene-targeted mice
    H Kawada
    Department of Medicine, Hollings Cancer Center, Medical University of South Carolina, Charleston, USA
    Int J Hematol 73:463-8. 2001
    ..These results provide further evidence for the role of Fli-1 in the regulation of hematopoiesis and for c-mpl as a Fli-1 target gene...
  40. ncbi request reprint Reversible expression of CD34 by adult human bone marrow long-term engrafting hematopoietic stem cells
    Esmail D Zanjani
    Department of Animal Biotechnology, University of Nevada, Reno, Nev, USA
    Exp Hematol 31:406-12. 2003
    ....
  41. ncbi request reprint Hematopoietic origin of microglial and perivascular cells in brain
    David C Hess
    Department of Neurology, Medical College of Georgia, Augusta, GA 30912, USA
    Exp Neurol 186:134-44. 2004
    ....
  42. ncbi request reprint Origin of fibroblast colony-forming units
    Makio Ogawa
    Exp Hematol 35:1319-20. 2007

Research Grants14

  1. Tissue Reconstituting Potential of Human Stem Cells
    Makio Ogawa; Fiscal Year: 2007
    ..Specific Aim #3: To determine the developmental control of the tissue reconstituting potentials of the stem cells in neonatal and adult mouse xenografts. ..
  2. Tissue Reconstituting Potential of Human Stem Cells
    Makio Ogawa; Fiscal Year: 2006
    ..Specific Aim #3: To determine the developmental control of the tissue reconstituting potentials of the stem cells in neonatal and adult mouse xenografts. ..
  3. Transdifferentiation Potential of Bone Marrow Stem Cells
    Makio Ogawa; Fiscal Year: 2006
    ..abstract_text> ..
  4. Tissue Reconstituting Potential of Human Stem Cells
    Makio Ogawa; Fiscal Year: 2005
    ..Specific Aim #3: To determine the developmental control of the tissue reconstituting potentials of the stem cells in neonatal and adult mouse xenografts. ..
  5. Transdifferentiation Potential of Bone Marrow Stem Cells
    Makio Ogawa; Fiscal Year: 2005
    ..abstract_text> ..
  6. Transdifferentiation Potential of Bone Marrow Stem Cells
    Makio Ogawa; Fiscal Year: 2004
    ..abstract_text> ..
  7. Transdifferentiation Potential of Bone Marrow Stem Cells
    Makio Ogawa; Fiscal Year: 2003
    ..abstract_text> ..
  8. IDENTIFICATION AND PURIFICATION OF HUMAN STEM CELLS
    Makio Ogawa; Fiscal Year: 2001
    ..NOD/SCID mouse model is more practical for limiting dilution studies. We already have exciting preliminary data regarding the use of myeloablated NOD/SCID newborn mice for quantitation of human engrafting cells. ..
  9. IDENTIFICATION AND PURIFICATION OF HUMAN STEM CELLS
    Makio Ogawa; Fiscal Year: 2000
    ..NOD/SCID mouse model is more practical for limiting dilution studies. We already have exciting preliminary data regarding the use of myeloablated NOD/SCID newborn mice for quantitation of human engrafting cells. ..
  10. IDENTIFICATION AND PURIFICATION OF HUMAN STEM CELLS
    Makio Ogawa; Fiscal Year: 1999
    ..NOD/SCID mouse model is more practical for limiting dilution studies. We already have exciting preliminary data regarding the use of myeloablated NOD/SCID newborn mice for quantitation of human engrafting cells. ..
  11. Hematopoietic Origin of Mesenchymal Cells
    Makio Ogawa; Fiscal Year: 2009
    ..e. abilities to generate fat tissues, bones and cartilages. The knowledge gained from these studies will allow subsequent development of new, unique avenues of therapy for obesity and diseases of bones and joints. ..