Frederick S Nolte

Summary

Affiliation: Medical University of South Carolina
Country: USA

Publications

  1. ncbi request reprint Novel internal controls for real-time PCR assays
    Frederick S Nolte
    Clin Chem 50:801-2. 2004
  2. pmc Evaluation of a rapid and completely automated real-time reverse transcriptase PCR assay for diagnosis of enteroviral meningitis
    Frederick S Nolte
    Emory University School of Medicine, Atlanta, Georgia, USA
    J Clin Microbiol 49:528-33. 2011
  3. pmc MultiCode-PLx system for multiplexed detection of seventeen respiratory viruses
    Frederick S Nolte
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA
    J Clin Microbiol 45:2779-86. 2007
  4. doi request reprint Molecular diagnostics for detection of bacterial and viral pathogens in community-acquired pneumonia
    Frederick S Nolte
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425 9080, USA
    Clin Infect Dis 47:S123-6. 2008
  5. pmc Comparison of the sensitivities and viral load values of the AMPLICOR HIV-1 MONITOR version 1.0 and 1.5 tests
    Angela M Caliendo
    Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA
    J Clin Microbiol 42:5392-3. 2004
  6. pmc Multicenter beta trial of the GeneXpert enterovirus assay
    Christine B Kost
    Emory University Hospital, 1365 Clifton Road N E, Atlanta, GA 30322, USA
    J Clin Microbiol 45:1081-6. 2007
  7. doi request reprint Detection of respiratory viruses with a multiplex polymerase chain reaction assay (MultiCode-PLx Respiratory Virus Panel) in patients with hematologic malignancies
    Sujatha Murali
    Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30308, USA
    Leuk Lymphoma 50:619-24. 2009
  8. pmc Performance characteristics of a quantitative TaqMan hepatitis C virus RNA analyte-specific reagent
    James M Barbeau
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA
    J Clin Microbiol 42:3739-46. 2004
  9. doi request reprint Validation of an Epstein-Barr viral load assay using the QIAGEN Artus EBV TM PCR analyte-specific reagent
    Arshad N Ahsanuddin
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Emory University Hospital, Atlanta, GA 30322, USA
    Am J Clin Pathol 130:865-9. 2008
  10. ncbi request reprint Performance characteristics of two real-time PCR assays for the quantification of Epstein-Barr virus DNA
    Charles E Hill
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 1364 Clifton Road, Atlanta, GA 30322, USA
    Am J Clin Pathol 125:665-71. 2006

Collaborators

Detail Information

Publications22

  1. ncbi request reprint Novel internal controls for real-time PCR assays
    Frederick S Nolte
    Clin Chem 50:801-2. 2004
  2. pmc Evaluation of a rapid and completely automated real-time reverse transcriptase PCR assay for diagnosis of enteroviral meningitis
    Frederick S Nolte
    Emory University School of Medicine, Atlanta, Georgia, USA
    J Clin Microbiol 49:528-33. 2011
    ..The simplicity and on-demand capability of the Xpert EV assay should prove to be a valuable adjunct to the evaluation of suspected meningitis cases...
  3. pmc MultiCode-PLx system for multiplexed detection of seventeen respiratory viruses
    Frederick S Nolte
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA
    J Clin Microbiol 45:2779-86. 2007
    ..The MultiCode-PLx system provides clinical laboratories with a practical, rapid, and sensitive means for the massively multiplexed molecular detection of common respiratory viruses...
  4. doi request reprint Molecular diagnostics for detection of bacterial and viral pathogens in community-acquired pneumonia
    Frederick S Nolte
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425 9080, USA
    Clin Infect Dis 47:S123-6. 2008
    ..Consideration should be given to the development and evaluation of companion molecular diagnostic tests for detection of respiratory pathogens in future clinical trials of antimicrobials intended to treat community-acquired pneumonia...
  5. pmc Comparison of the sensitivities and viral load values of the AMPLICOR HIV-1 MONITOR version 1.0 and 1.5 tests
    Angela M Caliendo
    Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA
    J Clin Microbiol 42:5392-3. 2004
    ..5 tests are more sensitive than the microwell version 1.0 test. Viral load values obtained with the COBAS version 1.5 test are lower than those obtained with either the microwell version 1.0 or microwell version 1.5 test...
  6. pmc Multicenter beta trial of the GeneXpert enterovirus assay
    Christine B Kost
    Emory University Hospital, 1365 Clifton Road N E, Atlanta, GA 30322, USA
    J Clin Microbiol 45:1081-6. 2007
    ..The GXEA had a sensitivity of 97.1% (95% confidence interval [CI], 84.7 to 99.9%) and a specificity of 100% (95% CI, 94.6 to 100%) for the diagnosis of enteroviral meningitis...
  7. doi request reprint Detection of respiratory viruses with a multiplex polymerase chain reaction assay (MultiCode-PLx Respiratory Virus Panel) in patients with hematologic malignancies
    Sujatha Murali
    Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30308, USA
    Leuk Lymphoma 50:619-24. 2009
    ..Improved diagnostics for respiratory viruses may lead to more effective management and better outcomes in this patient population...
  8. pmc Performance characteristics of a quantitative TaqMan hepatitis C virus RNA analyte-specific reagent
    James M Barbeau
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA
    J Clin Microbiol 42:3739-46. 2004
    ..The performance characteristics of the TaqMan HCV ASR are appropriate for all clinical applications of HCV RNA testing...
  9. doi request reprint Validation of an Epstein-Barr viral load assay using the QIAGEN Artus EBV TM PCR analyte-specific reagent
    Arshad N Ahsanuddin
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Emory University Hospital, Atlanta, GA 30322, USA
    Am J Clin Pathol 130:865-9. 2008
    ..8% to 1.6%. A comparison of 35 matched samples showed a small positive bias (0.35 log(10) copies/mL) for the Artus ASR relative to a laboratory-developed EBV viral load assay targeting the Bam H1-W region of the EBV genome...
  10. ncbi request reprint Performance characteristics of two real-time PCR assays for the quantification of Epstein-Barr virus DNA
    Charles E Hill
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 1364 Clifton Road, Atlanta, GA 30322, USA
    Am J Clin Pathol 125:665-71. 2006
    ..Further clinical evaluation is needed to determine the viral load cutoff that is predictive of posttransplantation lymphoproliferative disorders...
  11. pmc Assessment of agreement between the AMPLICOR HIV-1 MONITOR test versions 1.0 and 1.5
    Charles E Hill
    Emory University Hospital and Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Clin Microbiol 42:286-9. 2004
    ....
  12. ncbi request reprint Detection and identification of cytochrome P-450 2C9 alleles *1, *2, and *3 by high-resolution melting curve analysis of PCR amplicons
    Charles E Hill
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
    Am J Clin Pathol 125:584-91. 2006
    ..67 and 29.56 vs 42.81 mg/wk; P<.05). High-resolution melting analysis provides a simple and accurate method for genotyping of CYP2C9...
  13. ncbi request reprint Risk factors for perinatal transmission of hepatitis C virus (HCV) and the natural history of HCV infection acquired in infancy
    Eric E Mast
    Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    J Infect Dis 192:1880-9. 2005
    ..The goal of the present study was to assess risk factors for perinatal hepatitis C virus (HCV) transmission and the natural history of infection among HCV-infected infants...
  14. pmc Correlation of penicillin Binding protein 2a detection with oxacillin resistance in Staphylococcus aureus and discovery of a novel penicillin binding protein 2a mutation
    Adam M Bressler
    Deparment of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA 30322, USA
    J Clin Microbiol 43:4541-4. 2005
    ..Both methods provided reliable detection of oxacillin-resistant S. aureus and facilitated the discovery of a novel, functionally impaired form of PBP2a...
  15. pmc Development and verification of an automated sample processing protocol for quantitation of human immunodeficiency virus type 1 RNA in plasma
    Brenda G Lee
    Clinical Laboratories, Emory University Hospital, Atlanta, Georgia 30322, USA
    J Clin Microbiol 41:2062-7. 2003
    ..In conclusion, the automated sample preparation protocol can replace both the standard and the ultrasensitive manual methods used with the AMPLICOR HIV-1 MONITOR test and can substantially reduce the labor associated with this test...
  16. ncbi request reprint Case studies in cost effectiveness of molecular diagnostics for infectious diseases: pulmonary tuberculosis, enteroviral meningitis, and BK virus nephropathy
    Frederick S Nolte
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
    Clin Infect Dis 43:1463-7. 2006
    ....
  17. pmc Preclinical evaluation of two real-time, reverse transcription-PCR assays for detection of the severe acute respiratory syndrome coronavirus
    Adam M Bressler
    Department of Medicine Infectious Diseases, and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA
    J Clin Microbiol 42:987-91. 2004
    ..The armored RNA was found to be a suitable positive control for the ORF 1b assay that could be reliably recovered and amplified from a variety of clinical specimens...
  18. pmc Development and evaluation of a real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase genes
    Justin M Cole
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Clin Microbiol 47:322-6. 2009
    ..The real-time PCR assay is a rapid and accurate method to detect all KPC isoenzymes and was useful in documenting the presence and dissemination of KPC-producing strains in our patient population...
  19. pmc Use of real-time polymerase chain reaction to identify cell- and tissue-type-selective peptides by phage display
    David L Jaye
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA
    Am J Pathol 162:1419-29. 2003
    ..Furthermore, identification of phage that bind specifically to diseased or cancerous tissue sections will likely be facilitated by this PCR-based approach...
  20. pmc Reproducibility of positive test results in the BDProbeTec ET system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae
    Elizabeth E Culler
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA
    J Clin Microbiol 41:3911-4. 2003
    ..7%. The data suggest that retesting of low-positive samples is warranted and could reduce the number of potentially false-positive test results...
  21. pmc Clinical evaluation of two methods for genotyping hepatitis C virus based on analysis of the 5' noncoding region
    Frederick S Nolte
    Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
    J Clin Microbiol 41:1558-64. 2003
    ..In conclusion, the performance characteristics of the 5'NC methods were similar and both methods produced accurate results at the genotype level but neither method should be used for subtyping...
  22. pmc Evaluation of a multiplexed PCR assay for detection of respiratory viral pathogens in a public health laboratory setting
    David J Marshall
    EraGen Biosciences Inc, Madison, WI 53717, USA
    J Clin Microbiol 45:3875-82. 2007
    ..In total, the PLx-RVP assay detected an additional 40 viral infections, of which 11 were mixed infections...