Research Topics
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| Frederick S NolteSummaryAffiliation: Medical University of South Carolina Country: USA Publications
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Detail Information
Publications
Novel internal controls for real-time PCR assaysFrederick S Nolte
Clin Chem 50:801-2. 2004
Evaluation of a rapid and completely automated real-time reverse transcriptase PCR assay for diagnosis of enteroviral meningitisFrederick S Nolte
Emory University School of Medicine, Atlanta, Georgia, USA
J Clin Microbiol 49:528-33. 2011..The simplicity and on-demand capability of the Xpert EV assay should prove to be a valuable adjunct to the evaluation of suspected meningitis cases...- MultiCode-PLx system for multiplexed detection of seventeen respiratory virusesFrederick S Nolte
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA
J Clin Microbiol 45:2779-86. 2007..The MultiCode-PLx system provides clinical laboratories with a practical, rapid, and sensitive means for the massively multiplexed molecular detection of common respiratory viruses... 
Molecular diagnostics for detection of bacterial and viral pathogens in community-acquired pneumoniaFrederick S Nolte
Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425 9080, USA
Clin Infect Dis 47:S123-6. 2008..Consideration should be given to the development and evaluation of companion molecular diagnostic tests for detection of respiratory pathogens in future clinical trials of antimicrobials intended to treat community-acquired pneumonia...- Comparison of the sensitivities and viral load values of the AMPLICOR HIV-1 MONITOR version 1.0 and 1.5 testsAngela M Caliendo
Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA
J Clin Microbiol 42:5392-3. 2004..5 tests are more sensitive than the microwell version 1.0 test. Viral load values obtained with the COBAS version 1.5 test are lower than those obtained with either the microwell version 1.0 or microwell version 1.5 test... - Multicenter beta trial of the GeneXpert enterovirus assayChristine B Kost
Emory University Hospital, 1365 Clifton Road N E, Atlanta, GA 30322, USA
J Clin Microbiol 45:1081-6. 2007..The GXEA had a sensitivity of 97.1% (95% confidence interval [CI], 84.7 to 99.9%) and a specificity of 100% (95% CI, 94.6 to 100%) for the diagnosis of enteroviral meningitis... - Detection of respiratory viruses with a multiplex polymerase chain reaction assay (MultiCode-PLx Respiratory Virus Panel) in patients with hematologic malignanciesSujatha Murali
Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30308, USA
Leuk Lymphoma 50:619-24. 2009..Improved diagnostics for respiratory viruses may lead to more effective management and better outcomes in this patient population... - Performance characteristics of a quantitative TaqMan hepatitis C virus RNA analyte-specific reagentJames M Barbeau
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA, USA
J Clin Microbiol 42:3739-46. 2004..The performance characteristics of the TaqMan HCV ASR are appropriate for all clinical applications of HCV RNA testing... - Validation of an Epstein-Barr viral load assay using the QIAGEN Artus EBV TM PCR analyte-specific reagentArshad N Ahsanuddin
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Emory University Hospital, Atlanta, GA 30322, USA
Am J Clin Pathol 130:865-9. 2008..8% to 1.6%. A comparison of 35 matched samples showed a small positive bias (0.35 log(10) copies/mL) for the Artus ASR relative to a laboratory-developed EBV viral load assay targeting the Bam H1-W region of the EBV genome... - Performance characteristics of two real-time PCR assays for the quantification of Epstein-Barr virus DNACharles E Hill
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 1364 Clifton Road, Atlanta, GA 30322, USA
Am J Clin Pathol 125:665-71. 2006..Further clinical evaluation is needed to determine the viral load cutoff that is predictive of posttransplantation lymphoproliferative disorders... - Assessment of agreement between the AMPLICOR HIV-1 MONITOR test versions 1.0 and 1.5Charles E Hill
Emory University Hospital and Emory University School of Medicine, Atlanta, Georgia 30322, USA
J Clin Microbiol 42:286-9. 2004.... - Detection and identification of cytochrome P-450 2C9 alleles *1, *2, and *3 by high-resolution melting curve analysis of PCR ampliconsCharles E Hill
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
Am J Clin Pathol 125:584-91. 2006..67 and 29.56 vs 42.81 mg/wk; P<.05). High-resolution melting analysis provides a simple and accurate method for genotyping of CYP2C9... - Risk factors for perinatal transmission of hepatitis C virus (HCV) and the natural history of HCV infection acquired in infancyEric E Mast
Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
J Infect Dis 192:1880-9. 2005..The goal of the present study was to assess risk factors for perinatal hepatitis C virus (HCV) transmission and the natural history of infection among HCV-infected infants... - Correlation of penicillin Binding protein 2a detection with oxacillin resistance in Staphylococcus aureus and discovery of a novel penicillin binding protein 2a mutationAdam M Bressler
Deparment of Pathology and Laboratory Medicine, Emory University Hospital, Atlanta, GA 30322, USA
J Clin Microbiol 43:4541-4. 2005..Both methods provided reliable detection of oxacillin-resistant S. aureus and facilitated the discovery of a novel, functionally impaired form of PBP2a... - Development and verification of an automated sample processing protocol for quantitation of human immunodeficiency virus type 1 RNA in plasmaBrenda G Lee
Clinical Laboratories, Emory University Hospital, Atlanta, Georgia 30322, USA
J Clin Microbiol 41:2062-7. 2003..In conclusion, the automated sample preparation protocol can replace both the standard and the ultrasensitive manual methods used with the AMPLICOR HIV-1 MONITOR test and can substantially reduce the labor associated with this test... - Case studies in cost effectiveness of molecular diagnostics for infectious diseases: pulmonary tuberculosis, enteroviral meningitis, and BK virus nephropathyFrederick S Nolte
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
Clin Infect Dis 43:1463-7. 2006.... - Preclinical evaluation of two real-time, reverse transcription-PCR assays for detection of the severe acute respiratory syndrome coronavirusAdam M Bressler
Department of Medicine (Infectious Diseases, and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA
J Clin Microbiol 42:987-91. 2004..The armored RNA was found to be a suitable positive control for the ORF 1b assay that could be reliably recovered and amplified from a variety of clinical specimens... - Development and evaluation of a real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase genesJustin M Cole
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
J Clin Microbiol 47:322-6. 2009..The real-time PCR assay is a rapid and accurate method to detect all KPC isoenzymes and was useful in documenting the presence and dissemination of KPC-producing strains in our patient population... - Use of real-time polymerase chain reaction to identify cell- and tissue-type-selective peptides by phage displayDavid L Jaye
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA
Am J Pathol 162:1419-29. 2003..Furthermore, identification of phage that bind specifically to diseased or cancerous tissue sections will likely be facilitated by this PCR-based approach... - Reproducibility of positive test results in the BDProbeTec ET system for detection of Chlamydia trachomatis and Neisseria gonorrhoeaeElizabeth E Culler
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA
J Clin Microbiol 41:3911-4. 2003..7%. The data suggest that retesting of low-positive samples is warranted and could reduce the number of potentially false-positive test results... - Clinical evaluation of two methods for genotyping hepatitis C virus based on analysis of the 5' noncoding regionFrederick S Nolte
Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA
J Clin Microbiol 41:1558-64. 2003..In conclusion, the performance characteristics of the 5'NC methods were similar and both methods produced accurate results at the genotype level but neither method should be used for subtyping... - Evaluation of a multiplexed PCR assay for detection of respiratory viral pathogens in a public health laboratory settingDavid J Marshall
EraGen Biosciences Inc, Madison, WI 53717, USA
J Clin Microbiol 45:3875-82. 2007..In total, the PLx-RVP assay detected an additional 40 viral infections, of which 11 were mixed infections...