Radoslaw Michalski

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. pmc Hydropropidine: a novel, cell-impermeant fluorogenic probe for detecting extracellular superoxide
    Radoslaw Michalski
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Free Radic Biol Med 54:135-47. 2013
  2. pmc HPLC-based monitoring of products formed from hydroethidine-based fluorogenic probes--the ultimate approach for intra- and extracellular superoxide detection
    Balaraman Kalyanaraman
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI, USA Electronic address
    Biochim Biophys Acta 1840:739-44. 2014
  3. ncbi request reprint Real-time Measurements of Amino Acid and Protein Hydroperoxides Using Coumarin Boronic Acid
    Radoslaw Michalski
    From the Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 and the Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, 90 924 Lodz, Poland
    J Biol Chem 289:22536-53. 2014
  4. doi request reprint On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: a reassessment
    Radoslaw Michalski
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA Institute of Applied Radiation Chemistry, Lodz University of Technology, 90 924 Lodz, Poland
    Free Radic Biol Med 67:278-84. 2014

Detail Information

Publications4

  1. pmc Hydropropidine: a novel, cell-impermeant fluorogenic probe for detecting extracellular superoxide
    Radoslaw Michalski
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Free Radic Biol Med 54:135-47. 2013
    ..g., Pr(2+)-Pr(2+)). In contrast to HE, there was very little intracellular uptake of HPr(+). We conclude that HPr(+) is a useful probe for detecting O(2)(•-) and other one-electron oxidizing species in an extracellular milieu...
  2. pmc HPLC-based monitoring of products formed from hydroethidine-based fluorogenic probes--the ultimate approach for intra- and extracellular superoxide detection
    Balaraman Kalyanaraman
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI, USA Electronic address
    Biochim Biophys Acta 1840:739-44. 2014
    ..However, using HPLC-based assays, 2-OH-E(+) and analogous hydroxylated products can be easily detected and quickly separated from other oxidation products...
  3. ncbi request reprint Real-time Measurements of Amino Acid and Protein Hydroperoxides Using Coumarin Boronic Acid
    Radoslaw Michalski
    From the Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 and the Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, 90 924 Lodz, Poland
    J Biol Chem 289:22536-53. 2014
    ..This observation reveals a new mechanism for amino acid and protein hydroperoxide formation in biological systems. ..
  4. doi request reprint On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: a reassessment
    Radoslaw Michalski
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA Institute of Applied Radiation Chemistry, Lodz University of Technology, 90 924 Lodz, Poland
    Free Radic Biol Med 67:278-84. 2014
    ..Zhao et al., Free Radic. Biol. Med. 34:1359, 2003). We conclude that whether in vitro or in vivo, E(+) measured by HPLC or by fluorescence lifetime imaging is not a diagnostic marker product for O2(-) reaction with HE. ..