J X Ma

Summary

Affiliation: Medical University of South Carolina
Country: USA

Publications

  1. ncbi Identification of RPE65 in transformed kidney cells
    J X Ma
    Department of Ophthalmology, Medical University of South Carolina, Charleston 29425, USA
    FEBS Lett 452:199-204. 1999
  2. ncbi A visual pigment expressed in both rod and cone photoreceptors
    J Ma
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA
    Neuron 32:451-61. 2001
  3. ncbi Salamander UV cone pigment: sequence, expression, and spectral properties
    J X Ma
    Department of Ophthalmology, Medical University of South Carolina, Charleston 29403, USA
    Vis Neurosci 18:393-9. 2001
  4. ncbi Expression, purification, and MALDI analysis of RPE65
    J Ma
    Department of Ophthalmology, Medical University of South Carolina, Charleston 29425, USA
    Invest Ophthalmol Vis Sci 42:1429-35. 2001
  5. ncbi Cloning and localization of RPE65 mRNA in salamander cone photoreceptor cells1
    J Ma
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Ave, 7th Floor, P O Box 250676, Charleston, SC 29425, USA
    Biochim Biophys Acta 1443:255-61. 1998
  6. ncbi Kallikrein-binding protein inhibits retinal neovascularization and decreases vascular leakage
    G Gao
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Ave, Charleston, SC 29425, USA
    Diabetologia 46:689-98. 2003
  7. ncbi Cloning, sequence analysis and expression of the gene encoding the mouse bradykinin B2 receptor
    J X Ma
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425
    Gene 149:283-8. 1994
  8. ncbi Cloning and characterization of three salamander retinal G-protein beta subunits
    J C Ryan
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29403, USA
    Mol Vis 7:222-7. 2001
  9. ncbi Differences in the pharmacological activation of visual opsins
    T Isayama
    Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts 02114, USA
    Vis Neurosci 23:899-908. 2006
  10. ncbi Kallistatin in human ocular tissues: reduced levels in vitreous fluids from patients with diabetic retinopathy
    J X Ma
    Department of Ophthalmology, Medical University of South Carolina, Charleston 29425, USA
    Curr Eye Res 15:1117-23. 1996

Collaborators

Detail Information

Publications67

  1. ncbi Identification of RPE65 in transformed kidney cells
    J X Ma
    Department of Ophthalmology, Medical University of South Carolina, Charleston 29425, USA
    FEBS Lett 452:199-204. 1999
    ..These results demonstrated that transformed kidney cells express this retinoid processing protein, suggesting that these transformed cells may have an alternative retinoid metabolism not present in normal kidney cells...
  2. ncbi A visual pigment expressed in both rod and cone photoreceptors
    J Ma
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA
    Neuron 32:451-61. 2001
    ..This suggests that neither the cell topology or the transducin is sufficient to differentiate the rod and the cone response...
  3. ncbi Salamander UV cone pigment: sequence, expression, and spectral properties
    J X Ma
    Department of Ophthalmology, Medical University of South Carolina, Charleston 29403, USA
    Vis Neurosci 18:393-9. 2001
    ..We hypothesize that the small red shift with A2 could be due to a twist in the chromophore that essentially isolates the ring double bond(s) from conjugation with the rest of the polyene chain...
  4. ncbi Expression, purification, and MALDI analysis of RPE65
    J Ma
    Department of Ophthalmology, Medical University of South Carolina, Charleston 29425, USA
    Invest Ophthalmol Vis Sci 42:1429-35. 2001
    ..The purpose of the study was to develop methods for the expression of the protein, determine the accurate molecular weight of this expressed protein, and quantitate the amount of RPE65 in the bovine RPE...
  5. ncbi Cloning and localization of RPE65 mRNA in salamander cone photoreceptor cells1
    J Ma
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Ave, 7th Floor, P O Box 250676, Charleston, SC 29425, USA
    Biochim Biophys Acta 1443:255-61. 1998
    ..The results indicate that this potential retinoid processing protein is expressed in the cone photoreceptor cells but not in rods. Therefore, this protein may contribute to the unique retinoid processing capabilities in salamander cones...
  6. ncbi Kallikrein-binding protein inhibits retinal neovascularization and decreases vascular leakage
    G Gao
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Ave, Charleston, SC 29425, USA
    Diabetologia 46:689-98. 2003
    ..It specifically binds to tissue kallikrein and inhibits kallikrein activity. Our study was designed to test its effects on retinal neovascularization and vascular permeability...
  7. ncbi Cloning, sequence analysis and expression of the gene encoding the mouse bradykinin B2 receptor
    J X Ma
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425
    Gene 149:283-8. 1994
    ....
  8. ncbi Cloning and characterization of three salamander retinal G-protein beta subunits
    J C Ryan
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29403, USA
    Mol Vis 7:222-7. 2001
    ..Since two distinct transducin alpha subunits have been cloned from the salamander retina, we aimed to identify and characterize the G-protein beta (Gbeta) subunits that are involved in visual signal transduction in the salamander...
  9. ncbi Differences in the pharmacological activation of visual opsins
    T Isayama
    Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, Harvard Medical School, Boston, Massachusetts 02114, USA
    Vis Neurosci 23:899-908. 2006
    ..Thus opsin structure determines the identity of beta-ionone as an agonist or an inverse agonist and in that respect distinguishes the red-sensitive cone opsin from all others...
  10. ncbi Kallistatin in human ocular tissues: reduced levels in vitreous fluids from patients with diabetic retinopathy
    J X Ma
    Department of Ophthalmology, Medical University of South Carolina, Charleston 29425, USA
    Curr Eye Res 15:1117-23. 1996
    ..Kallistatin is a serine proteinase inhibitor, which binds to tissue kallikrein and inhibits its proteolytic activity. This study is to determine the expression, cellular localization and the potential function of kallistatin in the eye...
  11. ncbi Molecular cloning and analysis of the rat kallikrein-binding protein gene
    K X Chai
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425
    J Biol Chem 266:16029-36. 1991
    ..A genomic Southern blot using an RKBP cDNA probe revealed multiple bands suggesting that the RKBP gene belongs to a family of highly conserved genes...
  12. ncbi Structure and chromosomal localization of the gene (BDKRB2) encoding human bradykinin B2 receptor
    J X Ma
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425
    Genomics 23:362-9. 1994
    ..In a Southern blot analysis following reverse transcription and polymerase chain reaction, the human B2 receptor was found to be expressed in most human tissues...
  13. ncbi Tissue kallikrein-binding protein reduces blood pressure in transgenic mice
    L M Chen
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 271:27590-4. 1996
    ..Our findings suggest that RKBP may function as a vasodilator in vivo, independent of regulating the activity of tissue kallikrein...
  14. ncbi Intravitreal injection of plasminogen kringle 5, an endogenous angiogenic inhibitor, arrests retinal neovascularization in rats
    D Zhang
    Department of Ophthalmology, Medical University of South Carolina, Charleston 29403, USA
    Diabetologia 44:757-65. 2001
    ..CONCLUSION/INTERPRETATION: These observations suggest that intravitreal delivery of angiogenic inhibitors could have therapeutic benefits in neovascular diseases of the retina...
  15. ncbi Molecular cloning and characterization of rKlk10, a cDNA encoding T-kininogenase from rat submandibular gland and kidney
    J X Ma
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425
    Biochemistry 31:10922-8. 1992
    ..rKlk10 cDNAs cloned from the kidney and submandibular gland show sequence identity.(ABSTRACT TRUNCATED AT 250 WORDS)..
  16. ncbi Molecular cloning of TA16, a transcriptional repressor that may mediate glucocorticoid-induced growth arrest of leiomyosarcoma cells
    W Fan
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston 29425, USA
    Mol Endocrinol 11:1342-52. 1997
    ..This finding suggests that TA16 might participate in the mediation of glucocorticoid-induced cell cycle arrest in leiomyosarcoma cells...
  17. ncbi Salutary effect of pigment epithelium-derived factor in diabetic nephropathy: evidence for antifibrogenic activities
    Joshua J Wang
    Department of Medicine Endocrinology, University of Oklahoma Health Sciences Ceter, Oklahoma City, Oklahoma, USA
    Diabetes 55:1678-85. 2006
    ....
  18. ncbi Deletion of smooth muscle alpha-actin alters blood-retina barrier permeability and retinal function
    James J Tomasek
    Departments of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City 73104, USA
    Invest Ophthalmol Vis Sci 47:2693-700. 2006
    ....
  19. ncbi RPE65 gene delivery restores isomerohydrolase activity and prevents early cone loss in Rpe65-/- mice
    Ying Chen
    Department of Cell Biology, The University of Oklahoma, Health Sciences Center, Oklahoma City, Oklahoma, USA
    Invest Ophthalmol Vis Sci 47:1177-84. 2006
    ..The purpose of this study is to determine whether RPE65 gene delivery restores the isomerohydrolase activity and normal profile of endogenous retinoids in Rpe65-/- mice...
  20. ncbi Identification of conserved histidines and glutamic acid as key residues for isomerohydrolase activity of RPE65, an enzyme of the visual cycle in the retinal pigment epithelium
    Yusuke Takahashi
    Department of Medicine Endocrinology, The University of Oklahoma Health Sciences Center, BSEB 328B, Oklahoma City, 73104, USA
    FEBS Lett 579:5414-8. 2005
    ..Moreover, these mutations significantly reduced protein stability of RPE65. These results indicate that these conserved residues are essential for the isomerohydrolase activity of RPE65 and its stability...
  21. ncbi Downregulation of cone-specific gene expression and degeneration of cone photoreceptors in the Rpe65-/- mouse at early ages
    Sergey L Znoiko
    Departments of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina, USA
    Invest Ophthalmol Vis Sci 46:1473-9. 2005
    ..Rod photoreceptors in the RPE65-knockout (Rpe65(-/-)) mouse are known to degenerate slowly with age. This study was designed to examine cone photoreceptors and the expression of cone-specific genes in the Rpe65(-/-) mouse...
  22. ncbi Targeted expression of Cre recombinase to cone photoreceptors in transgenic mice
    Yun Zheng Le
    Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, USA
    Mol Vis 10:1011-8. 2004
    ..To circumvent this problem and dissect gene functions in the cone photoreceptors, we elected to generate cone photoreceptor specific cre transgenic mice...
  23. pmc RPE65 is the isomerohydrolase in the retinoid visual cycle
    Gennadiy Moiseyev
    Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Proc Natl Acad Sci U S A 102:12413-8. 2005
    ..Identification of the function of RPE65 will contribute to the understanding of the pathogenesis for retinal dystrophies associated with RPE65 mutations...
  24. ncbi Two point mutations of RPE65 from patients with retinal dystrophies decrease the stability of RPE65 protein and abolish its isomerohydrolase activity
    Yusuke Takahashi
    Department of Medicine Endocrinology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 281:21820-6. 2006
    ..These results suggest that these mutations may result in critical structural alterations of RPE65 protein, disrupt its membrane association, and consequently impair its isomerohydrolase activity, leading to retinal degeneration...
  25. ncbi Impacts of two point mutations of RPE65 from Leber's congenital amaurosis on the stability, subcellular localization and isomerohydrolase activity of RPE65
    Ying Chen
    The University of Oklahoma Health Sciences Center, Department of Medicine Endocrinology, Department of Cell Biology, 941 Stanton L Young Blvd, BSEB 328B, Oklahoma City, OK 73104, USA
    FEBS Lett 580:4200-4. 2006
    ..These observations suggest that the decreased protein stability and altered subcellular localization of RPE65 may represent a mechanism for these mutations to lead to vision loss in LCA patients...
  26. ncbi Pigment epithelium-derived factor downregulates vascular endothelial growth factor (VEGF) expression and inhibits VEGF-VEGF receptor 2 binding in diabetic retinopathy
    Sarah X Zhang
    Department of Medicine, Department of Cell Biology, University of Oklahoma Health Sciences Center, 941 Stanton L Young Blvd, Oklahoma City, OK, USA
    J Mol Endocrinol 37:1-12. 2006
    ..The decrease in PEDF levels in the retina is at least partially responsible for the increase in VEGF expression and subsequent vascular leakage and neovascularization in diabetes...
  27. ncbi Conditional gene knockout system in cone photoreceptors
    Yun Zheng Le
    Department of Cell Biology, University of Oklahoma Health Sciences Center, USA
    Adv Exp Med Biol 572:173-8. 2006
    ..In addition, we have not observed any apparent ectopic expression in HRGP-cre mice (Le et al., 2004); thus, conditional knockout of essential genes with these mice is not likely to cause any detrimental effect in non-ocular tissues...
  28. ncbi G-protein-coupled receptor rhodopsin regulates the phosphorylation of retinal insulin receptor
    Ammaji Rajala
    Department of Ophthalmology, University of Oklahoma Health Science Center, Oklahoma 73104, USA
    J Biol Chem 282:9865-73. 2007
    ..This is the first study demonstrating that rhodopsin can initiate signaling pathway(s) in addition to its classical phototransduction...
  29. pmc Retinoid processing in cone and Müller cell lines
    Yogita Kanan
    Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Exp Eye Res 86:344-54. 2008
    ..Alternatively, this could be due to the potential that rMC-1, as a transformed cell line, has stopped expressing the proteins needed for the regeneration of 11-cis retinoids...
  30. pmc RPE65 from cone-dominant chicken is a more efficient isomerohydrolase compared with that from rod-dominant species
    Gennadiy Moiseyev
    Department of Cell Biology, Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    J Biol Chem 283:8110-7. 2008
    ....
  31. pmc Safety and efficacy of gene transfer for Leber's congenital amaurosis
    Albert M Maguire
    Scheie Eye Institute, University of Pennsylvania, USA
    N Engl J Med 358:2240-8. 2008
    ..Although the follow-up was very short and normal vision was not achieved, this study provides the basis for further gene therapy studies in patients with LCA...
  32. ncbi Identification of RDH10, an All-trans Retinol Dehydrogenase, in Retinal Muller Cells
    Bill X Wu
    Department of Ophthalmology, Medical University of South Carolina, USA
    Invest Ophthalmol Vis Sci 45:3857-62. 2004
    ..To investigate the expression of RDH10, an all-trans retinol dehydrogenase identified in the retinal pigment epithelium (RPE), in retinal Muller cells...
  33. ncbi Does constitutive phosphorylation protect against photoreceptor degeneration in Rpe65-/- mice?
    Baerbel Rohrer
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC, USA
    Adv Exp Med Biol 533:221-7. 2003
    ....
  34. ncbi Down-regulation of RPE65 protein expression and promoter activity by retinoic acid
    Yumei Chen
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Vis 9:345-54. 2003
    ..To further understand retinoid metabolism in these cells and the control of RPE65 expression, HEK293 cells were used as a model to determine if retinoic acid (RA) affects RPE65 promoter activity...
  35. ncbi Down-regulation of vascular endothelial growth factor and up-regulation of pigment epithelium-derived factor: a possible mechanism for the anti-angiogenic activity of plasminogen kringle 5
    Guoquan Gao
    Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29403, USA
    J Biol Chem 277:9492-7. 2002
    ....
  36. ncbi A novel Xenopus SWS2, P434 visual pigment: structure, cellular location, and spectral analyses
    Alix G Darden
    Storm Eye Institute, Department of Ophthalmology, Medical University of South Carolina, Charleston, SC, USA
    Mol Vis 9:191-9. 2003
    ..The purpose of this study was to clone and characterize the green rod pigment in Xenopus laevis...
  37. ncbi Retinyl esters are the substrate for isomerohydrolase
    Gennadiy Moiseyev
    Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Biochemistry 42:2229-38. 2003
    ..g., at 36 weeks, the levels are 20x that of WT). Our data indicate that the retinyl esters are the substrate of the isomerization reaction...
  38. ncbi Correlation of regenerable opsin with rod ERG signal in Rpe65-/- mice during development and aging
    Baerbel Rohrer
    Department of Ophthalmology Medical University of South Carolina, 167 Ashley Avenue, Charleston, SC 29425, USA
    Invest Ophthalmol Vis Sci 44:310-5. 2003
    ..This project was designed to determine the amount of regenerable opsin in Rpe65-/- mice during development and aging, and to examine the function of this rhodopsin by electroretinography (ERG)...
  39. ncbi Cloning and characterization of a novel all-trans retinol short-chain dehydrogenase/reductase from the RPE
    Bill X Wu
    Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Invest Ophthalmol Vis Sci 43:3365-72. 2002
    ..This study was conducted to identify the atRDH that generates all-trans retinal in the RPE...
  40. ncbi 11-cis-retinal reduces constitutive opsin phosphorylation and improves quantum catch in retinoid-deficient mouse rod photoreceptors
    Zsolt Ablonczy
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Avenue, Charleston, SC 29425, USA
    J Biol Chem 277:40491-8. 2002
    ..Our results indicate that opsin, which has not been exposed to 11-cis-retinal, does not generate the activity generally associated with the bleached apoprotein...
  41. ncbi Identification of the RPE65 protein in mammalian cone photoreceptors
    Sergey L Znoiko
    Storm Eye Institute, Medical University of South Carolina, 167 Ashley Avenue, Charleston, SC 29425, USA
    Invest Ophthalmol Vis Sci 43:1604-9. 2002
    ..The purpose of the present study was to determine whether RPE65 is expressed at the protein level in mammalian cones, as well as in those of amphibians...
  42. pmc Isorhodopsin rather than rhodopsin mediates rod function in RPE65 knock-out mice
    Jie Fan
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Avenue, Charleston, SC 29425, USA
    Proc Natl Acad Sci U S A 100:13662-7. 2003
    ..These studies demonstrate that a pathway exists in the eye for the generation of 9-cis-retinal that is independent of RPE65 and light...
  43. ncbi Visual cycle retinoid processing proteins are present in HEK293S cells
    Yumei Chen
    Department of Ophthalmology, Medical University of South Carolina, 167 Ashley Avenue, 29425, Charleston, SC, USA
    Vision Res 43:3037-44. 2003
    ..Small interference RNA specific to LRAT reduced ester formation, confirming that the enzyme is present. Therefore, HEK293S cells contain the functional components of the retinoid cycle found in the RPE...
  44. ncbi Role of the 9-methyl group of retinal in cone visual pigments
    Joydip Das
    Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Biochemistry 43:5532-8. 2004
    ..However, for the red cone pigment, the 9-methyl group of retinal appears to be critical in the deactivation pathway...
  45. ncbi Suppression of corneal neovascularization by PEDF release from human amniotic membranes
    Chunkui Shao
    Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, USA
    Invest Ophthalmol Vis Sci 45:1758-62. 2004
    ..The purpose of the present study is to reveal the molecular basis underlying antiangiogenic activity of HAM...
  46. ncbi Plasminogen kringle 5 inhibits alkali-burn-induced corneal neovascularization
    Zhihong Zhang
    Zhongshan Ophthalmic Center, Zhongshan University, Guangzhou, People s Republic of China
    Invest Ophthalmol Vis Sci 46:4062-71. 2005
    ..Plasminogen kringle 5 (K5) is a potent angiogenic inhibitor. The purpose of the present study was to evaluate the therapeutic effect of K5 on alkali-burn-induced corneal neovascularization (NV) and to investigate its mechanism of action...
  47. ncbi Heat shock protein 27 delays Ca2+-induced cell death in a caspase-dependent and -independent manner in rat retinal ganglion cells
    N Andrew Whitlock
    Hewitt Laboratory of the Ola B Williams Glaucoma Center, Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA
    Invest Ophthalmol Vis Sci 46:1085-91. 2005
    ..The timing of this upregulation of Hsp27 correlates with the protective effects of the treatment. It was the goal of the current study to determine what role Hsp27 plays in this protection...
  48. pmc Genetic difference in susceptibility to the blood-retina barrier breakdown in diabetes and oxygen-induced retinopathy
    Sarah X Zhang
    Department of Medicine, The Oklahoma University Health Science Center, 941 Stanton L Young Blvd, BSEB 328B, Oklahoma City, OK 73104 5043, USA
    Am J Pathol 166:313-21. 2005
    ..This finding suggests that genetic backgrounds contribute to the susceptibility to diabetic retinopathy...
  49. pmc RDH10 is essential for synthesis of embryonic retinoic acid and is required for limb, craniofacial, and organ development
    Lisa L Sandell
    Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA
    Genes Dev 21:1113-24. 2007
    ..Furthermore, these results identify a new nodal point in RA metabolism during embryogenesis...
  50. ncbi Very low density lipoprotein receptor, a negative regulator of the wnt signaling pathway and choroidal neovascularization
    Ying Chen
    Department of Cell Biology, University of Oklahoma Health Sciences Center, 941 Stanton L Young Boulevard, Oklahoma City, OK 73104, USA
    J Biol Chem 282:34420-8. 2007
    ..These results suggest that VLDLR functions as a negative regulator of CNV, and this function is mediated through the wnt pathway...
  51. ncbi Kallikrein-binding protein inhibits growth of gastric carcinoma by reducing vascular endothelial growth factor production and angiogenesis
    Baohe Zhu
    Department of Biochemistry, Zhongshan Medical School, Sun Yat Sen University, No 74 ZhongShan Road II, Guangzhou 510089, China
    Mol Cancer Ther 6:3297-306. 2007
    ....
  52. ncbi Difference in ischemic regulation of vascular endothelial growth factor and pigment epithelium--derived factor in brown norway and sprague dawley rats contributing to different susceptibilities to retinal neovascularization
    Guoquan Gao
    Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29403, USA
    Diabetes 51:1218-25. 2002
    ..These observations support the idea that different regulation of angiogenic inhibitors and stimulators under ischemia are responsible for the differences in susceptibility to ischemia-induced retinal neovascularization in SD and BN rats...
  53. doi Anti-inflammatory effects of pigment epithelium-derived factor in diabetic nephropathy
    Joshua J Wang
    Department of Medicine Endocrinology and Cell Biology, Dean A McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Am J Physiol Renal Physiol 294:F1166-73. 2008
    ..These results suggest that the renoprotective effect of PEDF against diabetic nephropathy may be partially through its anti-inflammatory activity, likely by blocking the NF-kappaB and HIF-1 pathways...
  54. doi Intraretinal leakage and oxidation of LDL in diabetic retinopathy
    Mingyuan Wu
    Harold Hamm Oklahoma Diabetes Center and the Section of Endocrinology and Diabetes, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Invest Ophthalmol Vis Sci 49:2679-85. 2008
    ..The pathogenesis of diabetic retinopathy (DR) is not fully understood. Clinical studies suggest that dyslipidemia is associated with the initiation and progression of DR. However, no direct evidence supports this theory...
  55. ncbi Light induces programmed cell death by activating multiple independent proteases in a cone photoreceptor cell line
    Yogita Kanan
    Departments of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Invest Ophthalmol Vis Sci 48:40-51. 2007
    ..The objective of this study was to determine the molecular events leading to the death of photoreceptor cells...
  56. ncbi The roles of three palmitoylation sites of RPE65 in its membrane association and isomerohydrolase activity
    Yusuke Takahashi
    Department of Medicine Endocrinology, University of Oklahoma Health Sciences Center OUHSC, Oklahoma City, Oklahoma, USA
    Invest Ophthalmol Vis Sci 47:5191-6. 2006
    ..The purpose of this study was to define the role of these Cys residues in palmitoylation, membrane association, and isomerohydrolase activity of RPE65...
  57. ncbi RPE65 is an iron(II)-dependent isomerohydrolase in the retinoid visual cycle
    Gennadiy Moiseyev
    Department of Cell Biology, Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    J Biol Chem 281:2835-40. 2006
    ..Inductively coupled plasma mass spectrometry measurements showed that bovine RPE65 binds iron ion with a stoichiometry of 0.8 +/- 0.1. These results indicate that RPE65 is an iron-dependent isomerohydrolase in the visual cycle...
  58. ncbi Pigment epithelium-derived factor (PEDF) is an endogenous antiinflammatory factor
    Sarah X Zhang
    Department of Medicine Endocrinology, Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA
    FASEB J 20:323-5. 2006
    ..These findings suggest that PEDF is a novel endogenous anti-inflammatory factor in the eye. The decrease of ocular PEDF levels may contribute to inflammation and vascular leakage in DR...
  59. ncbi Inhibition of ocular angiogenesis by diced small interfering RNAs (siRNAs) specific to vascular endothelial growth factor (VEGF)
    Masatoshi Murata
    Mitake Eye Clinic, and Department of Biochemistry, School of Medicine, Iwate Medical University, Morioka, Japan
    Curr Eye Res 31:171-80. 2006
    ..Histological analysis displayed no particular tissue damage in the conjunctiva where siRNA was injected. The approach using diced siRNAs can be a new tool for various neovascular ocular diseases...
  60. ncbi Decreased expression of pigment epithelium-derived factor is involved in the pathogenesis of diabetic nephropathy
    Joshua J Wang
    Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA
    Diabetes 54:243-50. 2005
    ..Therefore, decreased expression of PEDF in diabetic kidneys may contribute to extracellular matrix overproduction and the development of diabetic nephropathy...
  61. ncbi Systemic and periocular deliveries of plasminogen kringle 5 reduce vascular leakage in rat models of oxygen-induced retinopathy and diabetes
    Sarah X Zhang
    Department of Medicine, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73104, USA
    Curr Eye Res 30:681-9. 2005
    ..The current study is to determine the effect of plasminogen kringle 5 (K5) on vascular leakage via systemic and periocular deliveries...
  62. ncbi Bone marrow lacks a transplantable progenitor for smooth muscle type alpha-actin-expressing cells
    Takafumi Yokota
    Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73190 3048, USA
    Stem Cells 24:13-22. 2006
    ..Our findings support earlier studies suggesting that the bone marrow microenvironment is difficult to transplant and indicate that hematopoietic stem cells are unlikely to give rise to alphaSMA-expressing progeny...
  63. ncbi The effect of angiostatin on vascular leakage and VEGF expression in rat retina
    Jing Sima
    Department of Medicine, The University of Oklahoma Health Sciences Center, 941 Stanton L Young Blvd, BSEB 328B, Oklahoma City, OK 73104, USA
    FEBS Lett 564:19-23. 2004
    ..These results suggest that the effect of angiostatin on vascular leakage is mediated, at least in part, through blockade of VEGF overexpression...
  64. ncbi Retinal preconditioning and the induction of heat-shock protein 27
    Yan Li
    Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Invest Ophthalmol Vis Sci 44:1299-304. 2003
    ..In the present study the cellular changes in levels of heat shock protein (Hsp)27, -70, and -90 mRNA and expression of Hsp in the rat retina associated with ischemic preconditioning were evaluated...
  65. ncbi Hsp27 upregulation by HIF-1 signaling offers protection against retinal ischemia in rats
    N Andrew Whitlock
    Hewitt Laboratory of the Ola B Williams Glaucoma Center, Department of Ophthalmology, Medical University of South Carolina, Charleston, SC 29425, USA
    Invest Ophthalmol Vis Sci 46:1092-8. 2005
    ..The regulatory mechanisms involved in the upregulation of Hsp27 after IPC are unknown. The purpose of this study was to explore the transcriptional events responsible for the upregulation of Hsp27 after IPC...
  66. ncbi The rat Apg3p/Aut1p homolog is upregulated by ischemic preconditioning in the retina
    Bill X Wu
    Department of Ophthalmology, Medical University of South Carolina, Charleston, SC, USA
    Mol Vis 12:1292-302. 2006
    ..In this study, we have identified and characterized the rat homolog of yeast Apg3p/Aut1p, an important autophagy protein encoded by the autophagy 3-like (APG3L) gene. We have also further characterized the homologous human APG3L gene...
  67. ncbi Decreased pigment epithelium-derived factor and increased vascular endothelial growth factor levels in pterygia
    Ji Jin
    Department of Ophthalmology, The Jinhua Central Hospital, Zhejiang, China
    Cornea 22:473-7. 2003
    ..This study compared expression levels of an angiogenic inhibitor, pigment epithelium-derived factor (PEDF), in pterygia with those in normal corneal and conjunctival tissues...