ROY LONG

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. pmc She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA
    R M Long
    Medical College of Wisconsin, Department of Microbiology and Molecular Genetics, 8701 Watertown Plank Road, Milwaukee, WI 53226 0509, USA
    EMBO J 19:6592-601. 2000
  2. pmc An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast
    R M Long
    Medical College of Wisconsin, Department of Microbiology and Molecular Genetics, Milwaukee, Wisconsin 53226, USA
    J Cell Biol 153:307-18. 2001
  3. ncbi request reprint RNA localization in yeast: moving towards a mechanism
    Graydon B Gonsalvez
    Department of Genetics, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA
    Biol Cell 97:75-86. 2005
  4. pmc She3p possesses a novel activity required for ASH1 mRNA localization in Saccharomyces cerevisiae
    Sharon M Landers
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Eukaryot Cell 8:1072-83. 2009
  5. ncbi request reprint Loc1p is required for efficient assembly and nuclear export of the 60S ribosomal subunit
    Carl R Urbinati
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Mol Genet Genomics 276:369-77. 2006
  6. pmc RNA-protein interactions promote asymmetric sorting of the ASH1 mRNA ribonucleoprotein complex
    Graydon B Gonsalvez
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    RNA 9:1383-99. 2003
  7. pmc Characterization of Mmp37p, a Saccharomyces cerevisiae mitochondrial matrix protein with a role in mitochondrial protein import
    Michelle R Gallas
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Mol Biol Cell 17:4051-62. 2006
  8. ncbi request reprint ASH1 mRNA anchoring requires reorganization of the Myo4p-She3p-She2p transport complex
    Graydon B Gonsalvez
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 279:46286-94. 2004
  9. pmc The mechanism of action of the Pseudomonas aeruginosa-encoded type III cytotoxin, ExoU
    Hiromi Sato
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    EMBO J 22:2959-69. 2003
  10. ncbi request reprint mRNA decay: x (XRN1) marks the spot
    Roy M Long
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Mol Cell 11:1126-8. 2003

Research Grants

  1. RNA LOCALIZATION IN S.CEREVISIAE
    ROY LONG; Fiscal Year: 2004

Collaborators

  • P Chartrand
  • Ming Lei
  • James Williamson
  • Rosemary Stuart
  • Graydon B Gonsalvez
  • Michelle R Gallas
  • Carl R Urbinati
  • Sharon M Landers
  • Hiromi Sato
  • Jaime Little
  • Mary K Dienhart
  • John P Aris
  • Jaime L Little
  • Jeanine Wiener-Kronish
  • Ravi R Pankhaniya
  • Dara W Frank
  • Derek K Ho
  • Katrina A Lehmann
  • Jimmy B Feix
  • Eleni S Stanitsa
  • Teiji Sawa
  • Cecilia J Hillard
  • Viviane Finck-Barban├žon
  • Adam Buchaklian
  • Kiyoshi Moriyama

Detail Information

Publications11

  1. pmc She2p is a novel RNA-binding protein that recruits the Myo4p-She3p complex to ASH1 mRNA
    R M Long
    Medical College of Wisconsin, Department of Microbiology and Molecular Genetics, 8701 Watertown Plank Road, Milwaukee, WI 53226 0509, USA
    EMBO J 19:6592-601. 2000
    ..By tethering a She3p-MS2 fusion protein to a reporter RNA containing MS2 binding sites, we observed that She2p is dispensable for She3p-MS2-dependent RNA localization...
  2. pmc An exclusively nuclear RNA-binding protein affects asymmetric localization of ASH1 mRNA and Ash1p in yeast
    R M Long
    Medical College of Wisconsin, Department of Microbiology and Molecular Genetics, Milwaukee, Wisconsin 53226, USA
    J Cell Biol 153:307-18. 2001
    ..We propose that efficient cytoplasmic ASH1 mRNA localization requires a previous interaction with specific nuclear factors...
  3. ncbi request reprint RNA localization in yeast: moving towards a mechanism
    Graydon B Gonsalvez
    Department of Genetics, Case Western Reserve University, 10900 Euclid Ave, Cleveland, OH 44106, USA
    Biol Cell 97:75-86. 2005
    ..This review aims to discuss mechanistic details of mRNA localization in S. cerevisiae...
  4. pmc She3p possesses a novel activity required for ASH1 mRNA localization in Saccharomyces cerevisiae
    Sharon M Landers
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Eukaryot Cell 8:1072-83. 2009
    ..The present study reveals that the current accepted model for ASH1 mRNA localization does not fully account for the function of She3p in ASH1 mRNA localization...
  5. ncbi request reprint Loc1p is required for efficient assembly and nuclear export of the 60S ribosomal subunit
    Carl R Urbinati
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Mol Genet Genomics 276:369-77. 2006
    ....
  6. pmc RNA-protein interactions promote asymmetric sorting of the ASH1 mRNA ribonucleoprotein complex
    Graydon B Gonsalvez
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    RNA 9:1383-99. 2003
    ....
  7. pmc Characterization of Mmp37p, a Saccharomyces cerevisiae mitochondrial matrix protein with a role in mitochondrial protein import
    Michelle R Gallas
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Mol Biol Cell 17:4051-62. 2006
    ....
  8. ncbi request reprint ASH1 mRNA anchoring requires reorganization of the Myo4p-She3p-She2p transport complex
    Graydon B Gonsalvez
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 279:46286-94. 2004
    ..These results are consistent with a model whereby anchoring of ASH1 mRNA requires molecular remodeling of the Myo4p-She3p-She2p heterotrimeric complex, a process that is apparently altered when mRNA is artificially tethered to She2p...
  9. pmc The mechanism of action of the Pseudomonas aeruginosa-encoded type III cytotoxin, ExoU
    Hiromi Sato
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    EMBO J 22:2959-69. 2003
    ..Recombinant ExoU demonstrated lipase activity in vitro, but only in the presence of a yeast extract. From these data we conclude that ExoU is a lipase that requires activation or modification by eukaryotic factors...
  10. ncbi request reprint mRNA decay: x (XRN1) marks the spot
    Roy M Long
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Mol Cell 11:1126-8. 2003
    ....
  11. doi request reprint Monitoring the temporal and spatial distribution of RNA in living yeast cells
    Roy M Long
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI, USA
    Methods Mol Biol 419:187-96. 2008
    ..Here, we present a protocol that tethers green fluorescent protein (GFP) to an mRNA of interest, allowing for the visualization of dynamic mRNA localization processes in living cells...

Research Grants5

  1. RNA LOCALIZATION IN S.CEREVISIAE
    ROY LONG; Fiscal Year: 2004
    ..With the power of yeast genetics, we are poised for significant progress into the mechanism controlling RNA localization. ..