Ming Lei

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. ncbi request reprint The MCM complex: its role in DNA replication and implications for cancer therapy
    Ming Lei
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Curr Cancer Drug Targets 5:365-80. 2005
  2. ncbi request reprint Two mcm3 mutations affect different steps in the initiation of DNA replication
    Ming Lei
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Biol Chem 277:30824-31. 2002
  3. ncbi request reprint Two bipartite NLSs mediate constitutive nuclear localization of Mcm10
    Rebekah Burich
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Curr Genet 44:195-201. 2003
  4. ncbi request reprint The FK506-binding protein, Fpr4, is an acidic histone chaperone
    Haijie Xiao
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, 53226, USA
    FEBS Lett 580:4357-64. 2006
  5. ncbi request reprint A novel zinc finger is required for Mcm10 homocomplex assembly
    Craig R Cook
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 278:36051-8. 2003
  6. ncbi request reprint Voltage-gated sodium channels potentiate the invasive capacities of human non-small-cell lung cancer cell lines
    Sebastien Roger
    INSERM, E0211, Tours, F 37000, France
    Int J Biochem Cell Biol 39:774-86. 2007
  7. ncbi request reprint Genetic and comparative transcriptome analysis of bromodomain factor 1 in the salt stress response of Saccharomyces cerevisiae
    Xiangyong Liu
    State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P R China
    Curr Microbiol 54:325-30. 2007
  8. ncbi request reprint Integration of hydrogels with hard and soft microstructures
    Ming Lei
    Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    J Nanosci Nanotechnol 7:780-9. 2007
  9. doi request reprint A shared docking motif in TRF1 and TRF2 used for differential recruitment of telomeric proteins
    Yong Chen
    Department of Biological Chemistry, University of Michigan Medical School, 1150 West Medical Center Drive, Ann Arbor, MI 48109, USA
    Science 319:1092-6. 2008
  10. pmc Normal cells, but not cancer cells, survive severe Plk1 depletion
    Xiaoqi Liu
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave, Cambridge, MA 02138, USA
    Mol Cell Biol 26:2093-108. 2006

Research Grants

Collaborators

  • Michael A McAlear
  • T R Cech
  • Jean Yves Le Guennec
  • Jing Li
  • Jun Zhou
  • Tianhua Zhou
  • Xiaoqi Liu
  • Titia de Lange
  • Yong Chen
  • Valérie Lallemand-Breitenbach
  • Sebastien Roger
  • Haijie Xiao
  • Shang Zhong Xu
  • Babak Ziaie
  • Rebekah Burich
  • Craig R Cook
  • Maria Carla Parrini
  • Laurent Peres
  • Megan Van Overbeek
  • Jun Zhu
  • Rihab Nasr
  • Shirine Benhenda
  • Jill R Donigian
  • Yuting Yang
  • Paul Baciu
  • Marion Jeanne
  • Hugues De The
  • Brian Raught
  • Aurélia Barascu
  • Pierre Besson
  • Pierre Ivan Raynal
  • Sophie Iochmann
  • Jerome Rollin
  • Yves Gruel
  • Philippe Bougnoux
  • Vaughn Jackson
  • Chenliang Xiong
  • Fanning Zeng
  • David J Beech
  • Asipu Sivaprasadarao
  • Bin Gao
  • Antonio Baldi
  • Ronald A Siegel
  • Yuandong Gu
  • Guosheng Kung
  • Brian F Volkman
  • Francis C Peterson
  • Bruce J Mayer
  • Stephen C Harrison

Detail Information

Publications18

  1. ncbi request reprint The MCM complex: its role in DNA replication and implications for cancer therapy
    Ming Lei
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Curr Cancer Drug Targets 5:365-80. 2005
    ..Discussions will also cover activities and structures of the complex that may be useful for the development of drug screens...
  2. ncbi request reprint Two mcm3 mutations affect different steps in the initiation of DNA replication
    Ming Lei
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Biol Chem 277:30824-31. 2002
    ..These observations indicate that Mcm3-10 is defective in a step before, and Mcm3-1 is defective in a step after the recruitment of the MCM2-7 complex to replication origins...
  3. ncbi request reprint Two bipartite NLSs mediate constitutive nuclear localization of Mcm10
    Rebekah Burich
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Curr Genet 44:195-201. 2003
    ..Thus, Mcm10 contains two functional NLSs and either is sufficient for the constitutive nuclear localization of Mcm10...
  4. ncbi request reprint The FK506-binding protein, Fpr4, is an acidic histone chaperone
    Haijie Xiao
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, 53226, USA
    FEBS Lett 580:4357-64. 2006
    ..These observations argue that Fpr4, while unique structurally, deposits histones onto DNA for nucleosome assembly through the well-established mechanism shared by other chaperones...
  5. ncbi request reprint A novel zinc finger is required for Mcm10 homocomplex assembly
    Craig R Cook
    Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 278:36051-8. 2003
    ..These observations suggest that homocomplex assembly is essential for Mcm10 function. Multisubunit Mcm10 homocomplexes may provide the structural basis for Mcm10 to interact with multiple subunits of the MCM2-7 hexamer...
  6. ncbi request reprint Voltage-gated sodium channels potentiate the invasive capacities of human non-small-cell lung cancer cell lines
    Sebastien Roger
    INSERM, E0211, Tours, F 37000, France
    Int J Biochem Cell Biol 39:774-86. 2007
    ..These channels could serve both as novel markers of the metastatic phenotype and as potential new therapeutic targets...
  7. ncbi request reprint Genetic and comparative transcriptome analysis of bromodomain factor 1 in the salt stress response of Saccharomyces cerevisiae
    Xiangyong Liu
    State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, P R China
    Curr Microbiol 54:325-30. 2007
    ..Our results provided further information about the regulatory mechanism involved in the salt stress response and adds new insight for understanding the biological functional of bromdomain-containing proteins in cellular processes...
  8. ncbi request reprint Integration of hydrogels with hard and soft microstructures
    Ming Lei
    Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    J Nanosci Nanotechnol 7:780-9. 2007
    ..In the third example, swelling and shrinking of a hydrogel embedded in a microfabricated valve structure leads to chemical gating of fluid motion through that valve. In all cases, the small size of the system enhances its response rate...
  9. doi request reprint A shared docking motif in TRF1 and TRF2 used for differential recruitment of telomeric proteins
    Yong Chen
    Department of Biological Chemistry, University of Michigan Medical School, 1150 West Medical Center Drive, Ann Arbor, MI 48109, USA
    Science 319:1092-6. 2008
    ..Conversely, the TRFH domain of TRF1, but not of TRF2, interacts with another shelterin-associated factor: PinX1...
  10. pmc Normal cells, but not cancer cells, survive severe Plk1 depletion
    Xiaoqi Liu
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave, Cambridge, MA 02138, USA
    Mol Cell Biol 26:2093-108. 2006
    ..Therefore, these data further support suggestions that Plk1 may be a feasible cancer therapy target...
  11. ncbi request reprint Generation of functional ion-channel tools by E3 targeting
    Shang Zhong Xu
    Membrane and Systems Biology Research Institute, University of Leeds, Leeds, LS2 9JT, UK
    Nat Biotechnol 23:1289-93. 2005
    ....
  12. ncbi request reprint Pak1 kinase homodimers are autoinhibited in trans and dissociated upon activation by Cdc42 and Rac1
    Maria Carla Parrini
    Laboratory of Molecular Medicine, Boston, MA 02115, USA
    Mol Cell 9:73-83. 2002
    ..Another ligand, betaPIX, can stably associate with dimerized Pak1. Dimerization does not facilitate Pak1 trans-phosphorylation. We conclude that the functional significance of dimerization is to allow trans-inhibition...
  13. pmc CCT chaperonin complex is required for the biogenesis of functional Plk1
    Xiaoqi Liu
    Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Ave, Cambridge, MA 02138, USA
    Mol Cell Biol 25:4993-5010. 2005
    ..Finally, the CCT-depleted cells reenter the cell cycle upon reintroduction of the purified constitutively active form of Plk1, indicating that Plk1 might be a CCT substrate...
  14. ncbi request reprint Structure of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection
    Ming Lei
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Nat Struct Mol Biol 11:1223-9. 2004
    ..pombe Pot1pN-ssDNA complex, whereas the second OB fold binds and protects the 3' end of the ssDNA. These results provide an atomic-resolution model for chromosome end-capping...
  15. ncbi request reprint Hard and soft micromachining for BioMEMS: review of techniques and examples of applications in microfluidics and drug delivery
    Babak Ziaie
    Department of Electrical and Computer Engineering, University of Minnesota, Minneapolis, MN 55455, USA
    Adv Drug Deliv Rev 56:145-72. 2004
    ..The most complex of these systems utilizes a very small hydrogel based on phenylboronic acid to control the flow of an insulin solution in response to changes in glucose concentration...
  16. ncbi request reprint DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA
    Ming Lei
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Nature 426:198-203. 2003
    ..The structure also explains how Pot1p avoids binding the vast excess of RNA in the nucleus...
  17. ncbi request reprint Cooperative binding of single-stranded telomeric DNA by the Pot1 protein of Schizosaccharomyces pombe
    Ming Lei
    Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, Colorado 80309 0215, USA
    Biochemistry 41:14560-8. 2002
    ..Together, the binding properties of Pot1pN suggest that the protein anchors itself at the very 3' end of a chromosome and then fills in very efficiently, coating the entire single-stranded overhang of the telomere...
  18. doi request reprint Arsenic degrades PML or PML-RARalpha through a SUMO-triggered RNF4/ubiquitin-mediated pathway
    Valérie Lallemand-Breitenbach
    Université de Paris 7 CNRS UMR 7151, Equipe Labellisée N11 Ligue Nationale Contre le Cancer, Hopital St Louis, 1, Av C Vellefaux 75475 Paris CEDEX 10 France
    Nat Cell Biol 10:547-55. 2008
    ..As PML SUMOylation recruits not only RNF4, ubiquitin and proteasomes, but also many SUMOylated proteins onto PML nuclear bodies, these domains could physically integrate the SUMOylation, ubiquitination and degradation pathways...

Research Grants5

  1. Defining the Role of Mcm10 in DNA Replication
    Ming Lei; Fiscal Year: 2005
    ..These studies will provide a unique perspective to the understanding of origin activity and the interactions between the origin and the replication fork. ..