SERGEY KRUPENKO

Summary

Affiliation: Medical University of South Carolina
Country: USA

Publications

  1. ncbi Aspartate 142 is involved in both hydrolase and dehydrogenase catalytic centers of 10-formyltetrahydrofolate dehydrogenase
    S A Krupenko
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 274:35777-84. 1999
  2. ncbi On the role of conserved histidine 106 in 10-formyltetrahydrofolate dehydrogenase catalysis: connection between hydrolase and dehydrogenase mechanisms
    S A Krupenko
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 276:24030-7. 2001
  3. ncbi 10-formyltetrahydrofolate dehydrogenase, one of the major folate enzymes, is down-regulated in tumor tissues and possesses suppressor effects on cancer cells
    Sergey A Krupenko
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425, USA
    Cell Growth Differ 13:227-36. 2002
  4. ncbi FDH: an aldehyde dehydrogenase fusion enzyme in folate metabolism
    Sergey A Krupenko
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, United States
    Chem Biol Interact 178:84-93. 2009
  5. ncbi Cysteine 707 is involved in the dehydrogenase activity site of rat 10-formyltetrahydrofolate dehydrogenase
    S A Krupenko
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee
    J Biol Chem 270:519-22. 1995
  6. ncbi Overexpression of functional hydrolase domain of rat liver 10-formyltetrahydrofolate dehydrogenase in Escherichia coli
    S A Krupenko
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, 37232, USA
    Protein Expr Purif 14:146-52. 1998
  7. ncbi ALDH1L1 inhibits cell motility via dephosphorylation of cofilin by PP1 and PP2A
    N V Oleinik
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA
    Oncogene 29:6233-44. 2010
  8. ncbi Ectopic expression of 10-formyltetrahydrofolate dehydrogenase in A549 cells induces G1 cell cycle arrest and apoptosis
    Natalia V Oleinik
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Cancer Res 1:577-88. 2003
  9. ncbi Crystal structures of the carboxyl terminal domain of rat 10-formyltetrahydrofolate dehydrogenase: implications for the catalytic mechanism of aldehyde dehydrogenases
    Yaroslav Tsybovsky
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Biochemistry 46:2917-29. 2007
  10. ncbi Cancer cells activate p53 in response to 10-formyltetrahydrofolate dehydrogenase expression
    Natalia V Oleinik
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    Biochem J 391:503-11. 2005

Research Grants

  1. FDH: A Novel Determinant of Tumor Suppression
    Sergey A Krupenko; Fiscal Year: 2010
  2. MECHANISM OF ACTION OF A MAJOR FOLATE ENZYME
    Sergey A Krupenko; Fiscal Year: 2010
  3. FDH: A Novel Determinant of Tumor Suppression
    SERGEY KRUPENKO; Fiscal Year: 2009
  4. MECHANISM OF ACTION OF A MAJOR FOLATE ENZYME
    SERGEY KRUPENKO; Fiscal Year: 2006
  5. MECHANISM OF ACTIONS OF A MAJOR FOLATE ENZYME
    SERGEY KRUPENKO; Fiscal Year: 2000
  6. MECHANISM OF ACTION OF A MAJOR FOLATE ENZYME
    Sergey A Krupenko; Fiscal Year: 2010

Collaborators

Detail Information

Publications19

  1. ncbi Aspartate 142 is involved in both hydrolase and dehydrogenase catalytic centers of 10-formyltetrahydrofolate dehydrogenase
    S A Krupenko
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    J Biol Chem 274:35777-84. 1999
    ....
  2. ncbi On the role of conserved histidine 106 in 10-formyltetrahydrofolate dehydrogenase catalysis: connection between hydrolase and dehydrogenase mechanisms
    S A Krupenko
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 276:24030-7. 2001
    ..We hypothesize that 10-formyl-THF dehydrogenase reaction is not an independent reaction but is a combination of hydrolase and aldehyde dehydrogenase reactions...
  3. ncbi 10-formyltetrahydrofolate dehydrogenase, one of the major folate enzymes, is down-regulated in tumor tissues and possesses suppressor effects on cancer cells
    Sergey A Krupenko
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425, USA
    Cell Growth Differ 13:227-36. 2002
    ..Hence, down-regulation of FDH in tumors is proposed to be one of the cellular mechanisms that enhance proliferation...
  4. ncbi FDH: an aldehyde dehydrogenase fusion enzyme in folate metabolism
    Sergey A Krupenko
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, United States
    Chem Biol Interact 178:84-93. 2009
    ....
  5. ncbi Cysteine 707 is involved in the dehydrogenase activity site of rat 10-formyltetrahydrofolate dehydrogenase
    S A Krupenko
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee
    J Biol Chem 270:519-22. 1995
    ..We conclude that cysteine 707 is directly involved in the active site of 10-FTHFDH responsible for dehydrogenase activity, and there is a separate site for the hydrolase activity...
  6. ncbi Overexpression of functional hydrolase domain of rat liver 10-formyltetrahydrofolate dehydrogenase in Escherichia coli
    S A Krupenko
    Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee, 37232, USA
    Protein Expr Purif 14:146-52. 1998
    ..These results show that the hydrolase amino-terminal domain of FDH can be overexpressed as a functional enzyme in E. coli cells and purified in one step by a simple chromatographic procedure...
  7. ncbi ALDH1L1 inhibits cell motility via dephosphorylation of cofilin by PP1 and PP2A
    N V Oleinik
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA
    Oncogene 29:6233-44. 2010
    ..Overall, this study shows the presence of distinct intracellular signaling pathways regulating motility in response to folate status and points toward mechanisms involving folates in promoting a malignant phenotype...
  8. ncbi Ectopic expression of 10-formyltetrahydrofolate dehydrogenase in A549 cells induces G1 cell cycle arrest and apoptosis
    Natalia V Oleinik
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    Mol Cancer Res 1:577-88. 2003
    ..These results indicate that FDH antiproliferative effects on A549 cells include both G(1) cell cycle arrest and caspase-dependent apoptosis...
  9. ncbi Crystal structures of the carboxyl terminal domain of rat 10-formyltetrahydrofolate dehydrogenase: implications for the catalytic mechanism of aldehyde dehydrogenases
    Yaroslav Tsybovsky
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Biochemistry 46:2917-29. 2007
    ..When reduced, the nicotinamide ring of NADP is displaced from the active site, restoring the contact between Cys707 and Glu673 and allowing the latter to activate the hydrolytic water molecule in deacylation...
  10. ncbi Cancer cells activate p53 in response to 10-formyltetrahydrofolate dehydrogenase expression
    Natalia V Oleinik
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    Biochem J 391:503-11. 2005
    ..This implies that activation of p53-dependent pathways is a general downstream mechanism in response to induction of FDH expression in p53 functional cancer cells...
  11. ncbi Modular organization of FDH: Exploring the basis of hydrolase catalysis
    Steven N Reuland
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Protein Sci 15:1076-84. 2006
    ..Our results further imply that Ile in place of Asn in the FDH hydrolase catalytic center is an important determinant for hydrolase catalysis as opposed to transferase catalysis...
  12. ncbi Disruption of a calmodulin central helix-like region of 10-formyltetrahydrofolate dehydrogenase impairs its dehydrogenase activity by uncoupling the functional domains
    Steven N Reuland
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 278:22894-900. 2003
    ..Our results suggest that flexibility within the putative helix is important for FDH function and could be a point for regulation of the enzyme...
  13. ncbi The inverse relationship between reduced folate carrier function and pemetrexed activity in a human colon cancer cell line
    Shrikanta Chattopadhyay
    Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Cancer Ther 5:438-49. 2006
    ..micro..
  14. ncbi Leucovorin-induced resistance against FDH growth suppressor effects occurs through DHFR up-regulation
    Natalia V Oleinik
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, P.O. Box 250509, Charleston, SC 29425, United States
    Biochem Pharmacol 72:256-66. 2006
    ..DHFR-induced repression of FDH catalysis could be an S phase-related metabolic adjustment that provides protection against FDH suppressor effects...
  15. ncbi The crystal structure of the hydrolase domain of 10-formyltetrahydrofolate dehydrogenase: mechanism of hydrolysis and its interplay with the dehydrogenase domain
    Alexander A Chumanevich
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 279:14355-64. 2004
    ..The structure also provides clues as to how, in the native enzyme, the hydrolase domain transfers its product to the dehydrogenase domain...
  16. ncbi Metabolic derangement of methionine and folate metabolism in mice deficient in methionine synthase reductase
    C Lee Elmore
    Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA
    Mol Genet Metab 91:85-97. 2007
    ..The different metabolite profiles in the various genetic mouse models for hyperhomocyst(e)inemia may be useful in understanding biological effects of elevated homocyst(e)ine...
  17. ncbi 10-formyltetrahydrofolate dehydrogenase requires a 4'-phosphopantetheine prosthetic group for catalysis
    Henry Donato
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA
    J Biol Chem 282:34159-66. 2007
    ..Thus, our study defines the intermediate domain of FDH as a novel carrier protein and provides the previously lacking component of the FDH catalytic mechanism...
  18. ncbi Crystallization and preliminary X-ray diffraction analysis of recombinant hydrolase domain of 10-formyltetrahydrofolate dehydrogenase
    Alexander A Chumanevich
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Acta Crystallogr D Biol Crystallogr 58:1841-2. 2002
    ..00, b = 64.63, c = 64.59 A. Based on the estimated solvent content, there is one 34 kDa molecule in the asymmetric unit. A native data set extending to 2.3 A resolution has been collected with good merging statistics...
  19. ncbi Methotrexate gamma-hydroxamate derivatives as potential dual target antitumor drugs
    M Amelia Santos
    Centro de Quimica Estrutural, Instituto Superior Tecnico, 1049 001 Lisboa, Portugal
    Bioorg Med Chem 15:1266-74. 2007
    ..Therefore, based on the present results, these bi-functional drugs may be good candidates to target specific tumors in animal models due to potential combined effects on two pathways crucial for tumor development...

Research Grants18

  1. FDH: A Novel Determinant of Tumor Suppression
    Sergey A Krupenko; Fiscal Year: 2010
    ..Since FDH can function as a restrictor of excessive proliferation, understanding its role in cellular metabolism will provide better understanding of tumorigenic processes. ..
  2. MECHANISM OF ACTION OF A MAJOR FOLATE ENZYME
    Sergey A Krupenko; Fiscal Year: 2010
    ....
  3. FDH: A Novel Determinant of Tumor Suppression
    SERGEY KRUPENKO; Fiscal Year: 2009
    ..It is proposed that investigation of the critical role of FDH in cancer cell survival will provide important insight into the malignant process itself and link disregulation of important metabolic pathways to cell death. ..
  4. MECHANISM OF ACTION OF A MAJOR FOLATE ENZYME
    SERGEY KRUPENKO; Fiscal Year: 2006
    ..The well known role of folate in prevention of megaloblastic anemia, vascular disease, neural tube birth defects and cancer make these studies particularly relevant. ..
  5. MECHANISM OF ACTIONS OF A MAJOR FOLATE ENZYME
    SERGEY KRUPENKO; Fiscal Year: 2000
    ....
  6. MECHANISM OF ACTION OF A MAJOR FOLATE ENZYME
    Sergey A Krupenko; Fiscal Year: 2010
    ....