Ashwani Khanna

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. ncbi request reprint Tacrolimus induces increased expression of transforming growth factor-beta1 in mammalian lymphoid as well as nonlymphoid cells
    A Khanna
    Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, 53226, USA
    Transplantation 67:614-9. 1999
  2. ncbi request reprint Recombinant p21 protein inhibits lymphocyte proliferation and transcription factors
    Ashwani K Khanna
    Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Immunol 174:7610-7. 2005
  3. ncbi request reprint Tacrolimus and cyclosporinein vitro and in vivo induce osteopontin mRNA and protein expression in renal tissues
    Ashwani Khanna
    Department of Medicine Nephrology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Nephron Exp Nephrol 101:e119-26. 2005
  4. pmc Reciprocal role of cyclins and cyclin kinase inhibitor p21WAF1/CIP1 on lymphocyte proliferation, allo-immune activation and inflammation
    Ashwani K Khanna
    Department of Medicine Nephrology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    BMC Immunol 6:22. 2005
  5. ncbi request reprint Concerted effect of transforming growth factor-beta, cyclin inhibitor p21, and c-myc on smooth muscle cell proliferation
    Ashwani Khanna
    Dept of Medicine, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 286:H1133-40. 2004
  6. ncbi request reprint The immunosuppressive agent tacrolimus induces p21WAF/CIP1WAF1/CIP1 via TGF-beta secretion
    Ashwani K Khanna
    Medical College of Wisconsin, 8701, Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochem Biophys Res Commun 303:266-72. 2003
  7. ncbi request reprint Expression of TGF-beta and fibrogenic genes in transplant recipients with tacrolimus and cyclosporine nephrotoxicity
    Ashwani Khanna
    Department of Medicine, Division of Nephrology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Kidney Int 62:2257-63. 2002
  8. ncbi request reprint Transforming growth factor (TGF)-beta mimics and anti-TGF-beta antibody abrogates the in vivo effects of cyclosporine: demonstration of a direct role of TGF-beta in immunosuppression and nephrotoxicity of cyclosporine
    A K Khanna
    The Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee 53226, USA
    Transplantation 67:882-9. 1999
  9. ncbi request reprint Cyclosporine induces the expression of the cyclin inhibitor p21
    A K Khanna
    Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee 53226, USA
    Transplantation 67:1262-8. 1999
  10. ncbi request reprint In vitro and in vivo transfection of p21 gene enhances cyclosporin A-mediated inhibition of lymphocyte proliferation
    A K Khanna
    The Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Immunol 165:1882-8. 2000

Detail Information

Publications27

  1. ncbi request reprint Tacrolimus induces increased expression of transforming growth factor-beta1 in mammalian lymphoid as well as nonlymphoid cells
    A Khanna
    Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, 53226, USA
    Transplantation 67:614-9. 1999
    ..In view of similarities between tacrolimus and CsA with respect to immunosuppressive mechanisms, we determined whether tacrolimus, in a fashion similar to CsA, induces TGF-beta1 hyperexpression in mammalian cells...
  2. ncbi request reprint Recombinant p21 protein inhibits lymphocyte proliferation and transcription factors
    Ashwani K Khanna
    Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Immunol 174:7610-7. 2005
    ....
  3. ncbi request reprint Tacrolimus and cyclosporinein vitro and in vivo induce osteopontin mRNA and protein expression in renal tissues
    Ashwani Khanna
    Department of Medicine Nephrology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Nephron Exp Nephrol 101:e119-26. 2005
    ..OPN may contribute to the CsA- and TAC-induced nephrotoxicity in organ transplant recipients and the increased OPN expression might be mediated by TGF-beta...
  4. pmc Reciprocal role of cyclins and cyclin kinase inhibitor p21WAF1/CIP1 on lymphocyte proliferation, allo-immune activation and inflammation
    Ashwani K Khanna
    Department of Medicine Nephrology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    BMC Immunol 6:22. 2005
    ..We hypothesize that the cell cycle control and inflammation are parallel events, inhibition of cellular proliferation by cyclin kinase inhibitor specifically p21 will limit inflammation and prevent allograft rejection...
  5. ncbi request reprint Concerted effect of transforming growth factor-beta, cyclin inhibitor p21, and c-myc on smooth muscle cell proliferation
    Ashwani Khanna
    Dept of Medicine, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 286:H1133-40. 2004
    ..This study uniquely analyzes the effect of TGF-beta at the molecular level on proliferation of SMC and on cell cycle regulatory molecules, implicating their potential role in the pathogenesis of atherosclerosis...
  6. ncbi request reprint The immunosuppressive agent tacrolimus induces p21WAF/CIP1WAF1/CIP1 via TGF-beta secretion
    Ashwani K Khanna
    Medical College of Wisconsin, 8701, Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochem Biophys Res Commun 303:266-72. 2003
    ..Strategies involving p21WAF/CIP1 induction should be considered a viable alternative strategy to achieve immunosuppression possibly with reduced toxicity associated with current immunosuppression...
  7. ncbi request reprint Expression of TGF-beta and fibrogenic genes in transplant recipients with tacrolimus and cyclosporine nephrotoxicity
    Ashwani Khanna
    Department of Medicine, Division of Nephrology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Kidney Int 62:2257-63. 2002
    ....
  8. ncbi request reprint Transforming growth factor (TGF)-beta mimics and anti-TGF-beta antibody abrogates the in vivo effects of cyclosporine: demonstration of a direct role of TGF-beta in immunosuppression and nephrotoxicity of cyclosporine
    A K Khanna
    The Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee 53226, USA
    Transplantation 67:882-9. 1999
    ..This study was planned to investigate whether anti-TGF-beta mitigated and TGF-beta reproduced the in vivo effects of CsA to directly prove this hypothesis...
  9. ncbi request reprint Cyclosporine induces the expression of the cyclin inhibitor p21
    A K Khanna
    Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee 53226, USA
    Transplantation 67:1262-8. 1999
    ..Because CsA induces the expression of transforming growth factor (TGF)-beta, and TGF-beta induces p21 expression, we also determined whether CsA's induction of p21 is dependent on or independent of TGF-beta...
  10. ncbi request reprint In vitro and in vivo transfection of p21 gene enhances cyclosporin A-mediated inhibition of lymphocyte proliferation
    A K Khanna
    The Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Immunol 165:1882-8. 2000
    ..This may serve as an alternate strategy to induce immunosuppression, perhaps with less toxicity than that which is seen with conventional immunosuppressive agents...
  11. ncbi request reprint Anti-transforming growth factor antibody at low but not high doses limits cyclosporine-mediated nephrotoxicity without altering rat cardiac allograft survival: potential of therapeutic applications
    Ashwani K Khanna
    Division of Nephrology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Circulation 110:3822-9. 2004
    ..Transforming growth factor-beta (TGF-beta) is one of the most potent mediators of the fibrogenic effects of cyclosporine...
  12. ncbi request reprint NADPH oxidase subunits (NOX-1, p22phox, Rac-1) and tacrolimus-induced nephrotoxicity in a rat renal transplant model
    Ashwani K Khanna
    Division of Cardiology, University of Maryland, 22 S Greene Street S 3B08, Baltimore, MD 21201, USA
    Nephrol Dial Transplant 22:376-85. 2007
    ..We examined whether anti-TGF-beta antibody limits nephrotoxicity produced by tacrolimus (TAC) and whether this altered genes that regulate oxidative stress...
  13. ncbi request reprint Post-translational modification of manganese superoxide dismutase in acutely rejecting cardiac transplants: role of inducible nitric oxide synthase
    Vani Nilakantan
    Division of Transplant Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Heart Lung Transplant 24:1591-9. 2005
    ..In this study, we examined the effect of inducible nitric oxide synthase (iNOS) on MnSOD expression, activity and nitration in acutely rejecting cardiac transplants...
  14. ncbi request reprint Antagonizing reactive oxygen by treatment with a manganese (III) metalloporphyrin-based superoxide dismutase mimetic in cardiac transplants
    Vani Nilakantan
    Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, Wis 53226, USA
    J Thorac Cardiovasc Surg 131:898-906. 2006
    ....
  15. pmc Nitric oxide formation in acutely rejecting cardiac allografts correlates with GTP cyclohydrolase I activity
    Galen M Pieper
    Division of Transplant Surgery, Department of Surgery, Medical College of Wisconsin, Milwaukee, WI, USA
    Biochem J 391:541-7. 2005
    ..These findings provide insight into a potential pathway regulating NO bioactivity in graft rejection. Such knowledge may potentially assist in the design of newer strategies to prevent acute graft rejection...
  16. ncbi request reprint Beneficial effect of alpha-tocopheryl succinate in rat cardiac transplants
    Thanh K Nguyen
    Department of Surgery, Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Heart Lung Transplant 25:707-15. 2006
    ..In this study we examined the effects of a novel vitamin E analog, alpha-tocopheryl polyethylene glycol-100 succinate (alpha-TPGS), and low-dose cyclosporine (CsA) in the treatment of acute and delayed cardiac rejection...
  17. ncbi request reprint Protective mechanisms of a metalloporphyrinic peroxynitrite decomposition catalyst, WW85, in rat cardiac transplants
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, USA
    J Pharmacol Exp Ther 314:53-60. 2005
    ..Rather, effects seem to be downstream on decreased protein nitration, decreased lipid peroxidation, and decreased PARP activation...
  18. ncbi request reprint Treatment with {alpha}-phenyl-N-tert-butylnitrone, a free radical-trapping agent, abrogates inflammatory cytokine gene expression during alloimmune activation in rat cardiac allografts
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 312:774-9. 2005
    ..Thus, PBN retains significant anti-inflammatory properties through its action to down-regulate cytokine gene expression that contribute to protection against acute alloimmune activation in cardiac allografts...
  19. ncbi request reprint TGF-beta1, FGF-2, and receptor mRNA expression in suture mesenchyme and dura versus underlying brain in fusing and nonfusing mouse cranial sutures
    Arun K Gosain
    Department of Plastic and Reconstructive Surgery, Medical College of Wisconsin, 9200 W Wisconsin Avenue, Milwaukee, WI 53226, USA
    Plast Reconstr Surg 113:1675-84. 2004
    ..Understanding these differences may eventually help to identify possible targets and time windows by which to most effectively modulate cranial suture fusion...
  20. ncbi request reprint Inhibition of nitrosylation, nitration, lymphocyte proliferation, and gene expression in acute and delayed cardiac allograft rejection by an orally active dithiocarbamate
    Galen M Pieper
    Department of Surgery Division of Transplant Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Cardiovasc Pharmacol 43:522-30. 2004
    ..These studies suggest that NOX-700 is protective in cardiac rejection, in part, by scavenging of NO and by limiting lymphocyte activation infiltration...
  21. ncbi request reprint P2Y purine receptor responses and expression in the pulmonary circulation of juvenile rabbits
    Girija G Konduri
    Department of Pediatrics, Medical College of Wisconsin, CHW OB 213 A, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 287:H157-64. 2004
    ..ATP appears to cause NO-mediated vasodilation predominantly through P2Y2 receptors on endothelium...
  22. ncbi request reprint Variable efficacy of N6-(1-iminoethyl)-L-lysine in acute cardiac transplant rejection
    Galen M Pieper
    Division of Transplant Surgery, Department of Surgery, Cardiovascular Center, Medical College of Wisconsin, Milwaukee 53226, USA
    Am J Physiol Heart Circ Physiol 286:H525-34. 2004
    ..These studies have important implications in understanding the dual role of NO in acute rejection and help to reconcile discrepancies in the literature...
  23. ncbi request reprint Mechanisms of the protective action of diethyldithiocarbamate-iron complex on acute cardiac allograft rejection
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 W Wisconsin Avenue, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 284:H1542-51. 2003
    ..Gene expression for the proinflammatory cytokine interferon-gamma was also decreased by DETC-Fe. Thus DETC-Fe limits NF-kappaB-dependent gene expression and possesses significant immunosuppressive properties...
  24. ncbi request reprint Analysis of transforming growth factor-beta and profibrogenic molecules in a rat cardiac allograft model treated with cyclosporine
    Ashwani K Khanna
    Department of Medicine Nephrology, Medical College of Wisconsin, Milwaukee, Wisconsin 53215, USA
    Transplantation 73:1543-9. 2002
    ....
  25. ncbi request reprint Prolonging organ allograft survival: potential role of nitric oxide scavengers
    Galen M Pieper
    Division of Transplant Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, WI 53226, USA
    BioDrugs 16:37-45. 2002
    ....
  26. pmc Targeted in vitro and in vivo gene transfer into T lymphocytes: potential of direct inhibition of allo-immune activation
    Ashwani K Khanna
    Department of Medicine, Division of Cardiology, University of Maryland, Baltimore, MD 21201, USA
    BMC Immunol 7:26. 2006
    ..Lymphocyte proliferation was quantified by 3H-thymidine uptake assay; IL-2 mRNA expression was studied by RT-PCR and using Real Time PCR assay, we monitored the CD3, p21, TNF-alpha and IFN-gamma mRNA expression...