Balaraman Kalyanaraman

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. pmc HPLC-based monitoring of products formed from hydroethidine-based fluorogenic probes--the ultimate approach for intra- and extracellular superoxide detection
    Balaraman Kalyanaraman
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI, USA Electronic address
    Biochim Biophys Acta 1840:739-44. 2014
  2. pmc Anti-inflammatory and neuroprotective effects of an orally active apocynin derivative in pre-clinical models of Parkinson's disease
    Anamitra Ghosh
    Department of Biomedical Sciences, Iowa Center for Advanced Neurotoxicology, Iowa State University, Ames, IA 50011, USA
    J Neuroinflammation 9:241. 2012
  3. pmc Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations
    Balaraman Kalyanaraman
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 52:1-6. 2012
  4. ncbi request reprint Iron signaling and oxidant damage
    Balaraman Kalyanaraman
    Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 0509, USA
    Cardiovasc Toxicol 7:92-4. 2007
  5. pmc Nitrated lipids: a class of cell-signaling molecules
    B Kalyanaraman
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Proc Natl Acad Sci U S A 101:11527-8. 2004
  6. doi request reprint Oxidative chemistry of fluorescent dyes: implications in the detection of reactive oxygen and nitrogen species
    Balaraman Kalyanaraman
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochem Soc Trans 39:1221-5. 2011
  7. pmc Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: another inconvenient truth
    Jacek Zielonka
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 48:983-1001. 2010
  8. ncbi request reprint Doxorubicin-induced apoptosis: implications in cardiotoxicity
    B Kalyanaraman
    Biophysics Research Institute and Free Radical Research Center, Medical College of Wisconsin, Milwaukee 53226, USA
    Mol Cell Biochem 234:119-24. 2002
  9. ncbi request reprint Reactive oxygen species induce reversible PECAM-1 tyrosine phosphorylation and SHP-2 binding
    Matthias Maas
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, PO Box 2178, Milwaukee, WI 53201 2178, USA
    Am J Physiol Heart Circ Physiol 285:H2336-44. 2003
  10. pmc Peroxynitrite is the major species formed from different flux ratios of co-generated nitric oxide and superoxide: direct reaction with boronate-based fluorescent probe
    Jacek Zielonka
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 285:14210-6. 2010

Research Grants

  1. Role of iNOS, Nitric Oxide & Arginase in Statin-Mediated Toxicity in Cancer Cells
    Balaraman Kalyanaraman; Fiscal Year: 2010
  2. Bicarbonate Enhances Peroxidation of SOD/ALS Mutants
    Balaraman Kalyanaraman; Fiscal Year: 2007
  3. Peroxide, NO and Iron Signaling in Endothelial Damage
    Balaraman Kalyanaraman; Fiscal Year: 2007
  4. ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINES
    Balaraman Kalyanaraman; Fiscal Year: 2006
  5. NITRIC OXIDE MEDICATED OXIDATION/NITRATION
    Balaraman Kalyanaraman; Fiscal Year: 2006
  6. Peroxide, NO and Iron Signaling in Endothelial Damage
    Balaraman Kalyanaraman; Fiscal Year: 2006
  7. Role of Neuronal NOS & Superoxide in Neurodegeneration
    Balaraman Kalyanaraman; Fiscal Year: 2006
  8. Bicarbonate Enhances Peroxidation of SOD/ALS Mutants
    Balaraman Kalyanaraman; Fiscal Year: 2006
  9. ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINES
    Balaraman Kalyanaraman; Fiscal Year: 2005
  10. Bicarbonate Enhances Peroxidation of SOD/ALS Mutants
    Balaraman Kalyanaraman; Fiscal Year: 2005

Collaborators

Detail Information

Publications57

  1. pmc HPLC-based monitoring of products formed from hydroethidine-based fluorogenic probes--the ultimate approach for intra- and extracellular superoxide detection
    Balaraman Kalyanaraman
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI, USA Electronic address
    Biochim Biophys Acta 1840:739-44. 2014
    ..However, using HPLC-based assays, 2-OH-E(+) and analogous hydroxylated products can be easily detected and quickly separated from other oxidation products...
  2. pmc Anti-inflammatory and neuroprotective effects of an orally active apocynin derivative in pre-clinical models of Parkinson's disease
    Anamitra Ghosh
    Department of Biomedical Sciences, Iowa Center for Advanced Neurotoxicology, Iowa State University, Ames, IA 50011, USA
    J Neuroinflammation 9:241. 2012
    ....
  3. pmc Measuring reactive oxygen and nitrogen species with fluorescent probes: challenges and limitations
    Balaraman Kalyanaraman
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 52:1-6. 2012
    ..We have proposed guidelines that will help present and future researchers with regard to the optimal use of selected fluorescent probes and interpretation of results...
  4. ncbi request reprint Iron signaling and oxidant damage
    Balaraman Kalyanaraman
    Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 0509, USA
    Cardiovasc Toxicol 7:92-4. 2007
    ..In this brief essay we discuss the interrelationship between the cellular signaling effects induced by extracellular and intracellular ROS and nitric oxide in the context of doxorubicin (DOX) toxicity...
  5. pmc Nitrated lipids: a class of cell-signaling molecules
    B Kalyanaraman
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Proc Natl Acad Sci U S A 101:11527-8. 2004
  6. doi request reprint Oxidative chemistry of fluorescent dyes: implications in the detection of reactive oxygen and nitrogen species
    Balaraman Kalyanaraman
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochem Soc Trans 39:1221-5. 2011
    ..Assays using fluorescent-based boronate probes will be more reliable for peroxynitrite determination than those using either dichlorodihydrofluorescein or dihydrorhodamine...
  7. pmc Hydroethidine- and MitoSOX-derived red fluorescence is not a reliable indicator of intracellular superoxide formation: another inconvenient truth
    Jacek Zielonka
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 48:983-1001. 2010
    ....
  8. ncbi request reprint Doxorubicin-induced apoptosis: implications in cardiotoxicity
    B Kalyanaraman
    Biophysics Research Institute and Free Radical Research Center, Medical College of Wisconsin, Milwaukee 53226, USA
    Mol Cell Biochem 234:119-24. 2002
    ..Here, we will focus on the role of eNOS expression, iron chelation, and iron signaling on DOX-mediated apoptosis...
  9. ncbi request reprint Reactive oxygen species induce reversible PECAM-1 tyrosine phosphorylation and SHP-2 binding
    Matthias Maas
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, PO Box 2178, Milwaukee, WI 53201 2178, USA
    Am J Physiol Heart Circ Physiol 285:H2336-44. 2003
    ..These findings support a role for PECAM-1 as a sensor of oxidative stress, perhaps most importantly during the process of inflammation...
  10. pmc Peroxynitrite is the major species formed from different flux ratios of co-generated nitric oxide and superoxide: direct reaction with boronate-based fluorescent probe
    Jacek Zielonka
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 285:14210-6. 2010
    ..We conclude that ONOO(-) is formed as the primary intermediate during the reaction between (*)NO and O(2) co-generated at different fluxes...
  11. pmc Mitochondria-targeted nitroxides exacerbate fluvastatin-mediated cytostatic and cytotoxic effects in breast cancer cells
    Gang Cheng
    Department of Biophysics, Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI, USA
    Cancer Biol Ther 12:707-17. 2011
    ..This strategy of combining mitochondria-targeted non-toxic molecules with cytotoxic chemotherapeutic drugs may be successfully used to enhance the efficacy of antitumor therapies in breast cancer treatment...
  12. pmc Mitochondria-targeted vitamin E analogs inhibit breast cancer cell energy metabolism and promote cell death
    Gang Cheng
    Free Radical Research Center and Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI, USA
    BMC Cancer 13:285. 2013
    ....
  13. pmc Mitochondria-targeted drugs synergize with 2-deoxyglucose to trigger breast cancer cell death
    Gang Cheng
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Cancer Res 72:2634-44. 2012
    ..Collectively, our findings suggest that dual targeting of mitochondrial bioenergetic metabolism with MTDs and glycolytic inhibitors such as 2-DG may offer a promising chemotherapeutic strategy...
  14. pmc Cytokines and lipopolysaccharides induce inducible nitric oxide synthase but not enzyme activity in adult rat cardiomyocytes
    Jeannette Vasquez-Vivar
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 45:994-1001. 2008
    ..This finding adds a new and unexpected layer of complexity to our understanding of the responses of the adult heart to inflammation...
  15. ncbi request reprint Increased arginase activity and endothelial dysfunction in human inflammatory bowel disease
    Scott Horowitz
    Dept of Medicine, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Gastrointest Liver Physiol 292:G1323-36. 2007
    ..Loss of .NO production in IBD microvessels is linked to enhanced levels of AR in intestinal endothelial cells exposed to chronic inflammation in vivo...
  16. pmc Mitochondria-targeted antioxidant and glycolysis inhibition: synergistic therapy in hepatocellular carcinoma
    Archana Dilip
    Department of Surgery, Division of Surgical Oncology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Anticancer Drugs 24:881-8. 2013
    ..The inability of the compounds to induce cytotoxicity or suppress the production of ATP in primary hepatocytes provides a selective and synergistic approach for the treatment of HCC...
  17. doi request reprint Real-time monitoring of reactive oxygen and nitrogen species in a multiwell plate using the diagnostic marker products of specific probes
    Jacek Zielonka
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Methods Enzymol 526:145-57. 2013
    ..By monitoring the diagnostic marker products formed from reaction between specific chemical probes and the oxidant species, it is possible to simultaneously assay these species using a multiwell plate (e.g., 384-well plate). ..
  18. ncbi request reprint Endothelial cell superoxide anion radical generation is not dependent on endothelial nitric oxide synthase-serine 1179 phosphorylation and endothelial nitric oxide synthase dimer/monomer distribution
    Jennifer Whitsett
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 40:2056-68. 2006
    ..We conclude that low BH4 but not inhibition of hsp90 increases O2*- in BAECs by mechanism(s) that unlikely involve phosphorylation to eNOS-S1179 or eNOS monomerization...
  19. pmc Boronate probes as diagnostic tools for real time monitoring of peroxynitrite and hydroperoxides
    Jacek Zielonka
    Department of Biophysics, Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Chem Res Toxicol 25:1793-9. 2012
    ..This perspective highlights potential applications of boronates and other fluorescent probes to high-throughput analyses of peroxynitrite and hydroperoxides in toxicological studies...
  20. ncbi request reprint Impact of pulmonary arterial endothelial cells on duroquinone redox status
    Marilyn P Merker
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, USA
    Free Radic Biol Med 37:86-103. 2004
    ..These endothelial redox functions and their apposition to the vessel lumen are consistent with the pulmonary endothelium being an important site of DQ reduction to DQH2 observed in the lungs...
  21. pmc Redox activation of Fe(III)-thiosemicarbazones and Fe(III)-bleomycin by thioredoxin reductase: specificity of enzymatic redox centers and analysis of reactive species formation by ESR spin trapping
    Judith M Myers
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 60:183-94. 2013
    ..The marked elevation of TrxR1 in many tumors could contribute to the selective tumor toxicity of these drugs by enhancing the redox activation of Fe(III)-thiosemicarbazones and the generation of reactive oxygen species such as HO...
  22. pmc Hydropropidine: a novel, cell-impermeant fluorogenic probe for detecting extracellular superoxide
    Radoslaw Michalski
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Free Radic Biol Med 54:135-47. 2013
    ..g., Pr(2+)-Pr(2+)). In contrast to HE, there was very little intracellular uptake of HPr(+). We conclude that HPr(+) is a useful probe for detecting O(2)(•-) and other one-electron oxidizing species in an extracellular milieu...
  23. pmc Reaction between peroxynitrite and boronates: EPR spin-trapping, HPLC Analyses, and quantum mechanical study of the free radical pathway
    Adam Sikora
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, United States
    Chem Res Toxicol 24:687-97. 2011
    ..Biological implications of the minor free radical pathway are discussed in the context of ONOO(-) detection, using the boronate probes...
  24. pmc Global profiling of reactive oxygen and nitrogen species in biological systems: high-throughput real-time analyses
    Jacek Zielonka
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 287:2984-95. 2012
    ....
  25. pmc Activation of nuclear factor-kappaB during doxorubicin-induced apoptosis in endothelial cells and myocytes is pro-apoptotic: the role of hydrogen peroxide
    Suwei Wang
    Biophysics Research Institute and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochem J 367:729-40. 2002
    ..Removal of intracellular H(2)O(2) protects endothelial cells and myocytes from DOX-induced apoptosis, possibly by inhibiting NF-kappaB activation. These findings suggest a novel mechanism for enhancing the therapeutic efficacy of DOX...
  26. pmc Differences in doxorubicin-induced apoptotic signaling in adult and immature cardiomyocytes
    Eugene A Konorev
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 45:1723-8. 2008
    ..This finding points to a major difference in the apoptotic signaling between immature and adult cardiomyocytes. The mitochondrial apoptotic pathway is limited in ARCM treated with DOX...
  27. doi request reprint Detection of 2-hydroxyethidium in cellular systems: a unique marker product of superoxide and hydroethidine
    Jacek Zielonka
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
    Nat Protoc 3:8-21. 2008
    ..As several oxidation products of HE are formed, the superoxide-specific product, 2-OH-E(+) needs to be separated from other HE-derived products for unequivocal quantification...
  28. ncbi request reprint Statin-induced breast cancer cell death: role of inducible nitric oxide and arginase-dependent pathways
    Srigiridhar Kotamraju
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Cancer Res 67:7386-94. 2007
    ..NO biosynthesis by NOS. We conclude that iNOS-mediated (.)NO is responsible in part for the proapoptotic, tumoricidal, and antiproliferative effects of statins in MCF-7 cells...
  29. ncbi request reprint Pulmonary arterial endothelial cells affect the redox status of coenzyme Q0
    Said H Audi
    Department of Biomedical Engineering, Marquette University, Milwaukee, WI, USA
    Free Radic Biol Med 34:892-907. 2003
    ..In addition, once formed the QH2 undergoes a cyanide-sensitive oxidation by the cells. Thus, the actual rate of Q reduction by the cells is greater than the net QH2 output from the cells...
  30. pmc Analysis of proteome changes in doxorubicin-treated adult rat cardiomyocyte
    Suresh N Kumar
    Department of Pathology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Proteomics 74:683-97. 2011
    ..Using a new solubilization technique, changes in low abundant protein profiles were monitored. Altered protein expression, modification and function related to oxidative stress response may play an important role in DOX cardiotoxicity...
  31. pmc Reaction between peroxynitrite and triphenylphosphonium-substituted arylboronic acid isomers: identification of diagnostic marker products and biological implications
    Adam Sikora
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Chem Res Toxicol 26:856-67. 2013
    ..We conclude that the ortho isomer probe reported here is most suitable for specific detection of ONOO(-) in biological systems...
  32. pmc A (99m)Tc-labeled triphenylphosphonium derivative for the early detection of breast tumors
    Zhixin Li
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Cancer Biother Radiopharm 24:579-87. 2009
    ..The aim of this study was to synthesize and characterize (99m)Tc-labeled alkyl triphenyl phosphonium ((99m)Tc-Mito(10)-MAG3) for the early detection of breast tumors...
  33. pmc Early detection of doxorubicin cardiomyopathy using two-dimensional strain echocardiography
    Raymond Q Migrino
    Cardiovascular Division, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Ultrasound Med Biol 34:208-14. 2008
    ..5, p=0.005). 2-dimensional radial strain echocardiography is useful in the early detection of doxorubicin cardiac injury and the reduction in radial strain is associated with histologic markers of doxorubicin cardiomyopathy...
  34. ncbi request reprint Mechanisms of endotoxin tolerance in human intestinal microvascular endothelial cells
    Hitoshi Ogawa
    Division of Gastroenterology and Hepatology, Department of Surgery, Milwaukee Veterans Administration Medical Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Immunol 170:5956-64. 2003
    ..HIMEC possess specific mechanisms of immunoregulatory hyporesponsiveness to repeated LPS exposure...
  35. ncbi request reprint Mitochondrially targeted antioxidants for the treatment of cardiovascular diseases
    Sharath Subramanian
    Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA
    Recent Pat Cardiovasc Drug Discov 5:54-65. 2010
    ..Although ongoing human clinical studies involve only non-cardiovascular applications at this time, preclinical studies show promise for eventual human trials for cardiovascular diseases...
  36. ncbi request reprint Real-time Measurements of Amino Acid and Protein Hydroperoxides Using Coumarin Boronic Acid
    Radoslaw Michalski
    From the Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 and the Institute of Applied Radiation Chemistry, Lodz University of Technology, Zeromskiego 116, 90 924 Lodz, Poland
    J Biol Chem 289:22536-53. 2014
    ..This observation reveals a new mechanism for amino acid and protein hydroperoxide formation in biological systems. ..
  37. doi request reprint On the use of fluorescence lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: a reassessment
    Radoslaw Michalski
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA Institute of Applied Radiation Chemistry, Lodz University of Technology, 90 924 Lodz, Poland
    Free Radic Biol Med 67:278-84. 2014
    ..Zhao et al., Free Radic. Biol. Med. 34:1359, 2003). We conclude that whether in vitro or in vivo, E(+) measured by HPLC or by fluorescence lifetime imaging is not a diagnostic marker product for O2(-) reaction with HE. ..
  38. pmc Diapocynin prevents early Parkinson's disease symptoms in the leucine-rich repeat kinase 2 (LRRK2R¹⁴⁴¹G) transgenic mouse
    Brian P Dranka
    Department of Biophysics, and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI, United States
    Neurosci Lett 549:57-62. 2013
    ..As expected, tyrosine hydroxylase staining was similar in both the substantia nigra and striatum in all treatment groups. Together these data demonstrate that diapocynin is a viable agent for protection of neurobehavioral function. ..
  39. ncbi request reprint Cyclooxygenase 2 rescues LNCaP prostate cancer cells from sanguinarine-induced apoptosis by a mechanism involving inhibition of nitric oxide synthase activity
    Jacob Huh
    Department of Medicine and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53266, USA
    Cancer Res 66:3726-36. 2006
    ....
  40. pmc The selenium-independent inherent pro-oxidant NADPH oxidase activity of mammalian thioredoxin reductase and its selenium-dependent direct peroxidase activities
    Qing Cheng
    Department of Pharmacology and Toxicology, MedicalCollege of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 285:21708-23. 2010
    ....
  41. doi request reprint An adenosine-mediated signaling pathway suppresses prenylation of the GTPase Rap1B and promotes cell scattering
    Elizabeth Ntantie
    1Department of Pharmacology and Toxicology, Cancer Center, and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Sci Signal 6:ra39. 2013
    ..Inhibiting A2B receptors may be an effective method to prevent metastasis...
  42. pmc Alterations in bioenergetic function induced by Parkinson's disease mimetic compounds: lack of correlation with superoxide generation
    Brian P Dranka
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI, USA
    J Neurochem 122:941-51. 2012
    ..Together these data indicate that MPP(+), rotenone, and 6-OHDA dramatically shift bioenergetic function away from the mitochondria and towards glycolysis in N27 cells...
  43. pmc Oxidation of histidine residues in copper-zinc superoxide dismutase by bicarbonate-stimulated peroxidase and thiol oxidase activities: pulse EPR and NMR studies
    Karunakaran Chandran
    Department of Biophysics, Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, United States
    Biochemistry 49:10616-22. 2010
    ..HCO(3)(-)-mediated, selective oxidative modification of histidines in SOD may be relevant to understanding the molecular mechanism of SOD peroxidase and thiol oxidase activities...
  44. pmc The ratio between tetrahydrobiopterin and oxidized tetrahydrobiopterin analogues controls superoxide release from endothelial nitric oxide synthase: an EPR spin trapping study
    Jeannette Vasquez-Vivar
    Biophysics Research Institute and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, U S A
    Biochem J 362:733-9. 2002
    ..NO formation. Collectively, these results indicate that the ratio between oxidized and reduced BH(4) metabolites tightly regulates superoxide formation from eNOS. The pathological significance of this scenario is discussed...
  45. ncbi request reprint Nitration of PECAM-1 ITIM tyrosines abrogates phosphorylation and SHP-2 binding
    Debra K Newman
    Blood Research Institute, The Blood Center of Southeastern Wisconsin, 53226, Milwaukee, WI, USA
    Biochem Biophys Res Commun 296:1171-9. 2002
    ..These data suggest that ITIM tyrosine nitration may represent a mechanism for modulating phosphotyrosine-dependent signal transduction pathways...
  46. doi request reprint Loss of bacillus Calmette-Guérin viability adversely affects the direct response of urothelial carcinoma cells to bacillus Calmette-Guérin exposure
    Gopitkumar Shah
    Departments of Urology and Biophysics BK, Medical College of Wisconsin, Milwaukee, Wisconsin
    J Urol 191:823-9. 2014
    ..We evaluated how loss of viability impacted a number of molecular and phenotypic intermediate end points that characterize and/or contribute to the direct effect of bacillus Calmette-Guérin on urothelial carcinoma cells...
  47. doi request reprint On the use of L-012, a luminol-based chemiluminescent probe, for detecting superoxide and identifying inhibitors of NADPH oxidase: a reevaluation
    Jacek Zielonka
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 65:1310-4. 2013
    ..These aspects make assays based on luminol analogs less than ideal for specific detection and identification of O2(*-) and NOX inhibitors...
  48. pmc Mechanism of metal-independent decomposition of organic hydroperoxides and formation of alkoxyl radicals by halogenated quinones
    Ben Zhan Zhu
    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People s Republic of China
    Proc Natl Acad Sci U S A 104:3698-702. 2007
    ..This represents a mechanism of organic alkoxyl radical formation not requiring the involvement of redox-active transition metal ions...
  49. ncbi request reprint Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen species
    Honglian Shi
    College of Pharmacy, University of New Mexico, Albuquerque, NM, USA
    Arch Biochem Biophys 437:59-68. 2005
    ..In addition, we observed that O2*- spin adducts were reduced to their corresponding hydroxyl adducts in the cellular system. The selection of optimal spin trap in trapping cell-generated O2*- is discussed...
  50. ncbi request reprint Metal-independent production of hydroxyl radicals by halogenated quinones and hydrogen peroxide: an ESR spin trapping study
    Ben Zhan Zhu
    Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA
    Free Radic Biol Med 32:465-73. 2002
    ..Metal-independent production of *OH from H(2)O(2) was also observed with several other halogenated quinones...
  51. pmc Molecular mechanism for metal-independent production of hydroxyl radicals by hydrogen peroxide and halogenated quinones
    Ben Zhan Zhu
    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, People s Republic of China
    Proc Natl Acad Sci U S A 104:17575-8. 2007
    ..This represents a mechanism of HO* production that does not require redox-active transition metal ions...
  52. ncbi request reprint NO adducts in mammalian red blood cells: too much or too little?
    Tienush Rassaf
    Nat Med 9:481-2; author reply 482-3. 2003
  53. ncbi request reprint Incorporation of the hydrophobic probe N-t-BOC-L-tyrosine tert-butyl ester to red blood cell membranes to study peroxynitrite-dependent reactions
    Natalia Romero
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Chem Res Toxicol 20:1638-48. 2007
    ....
  54. ncbi request reprint Mechanistic studies of peroxynitrite-mediated tyrosine nitration in membranes using the hydrophobic probe N-t-BOC-L-tyrosine tert-butyl ester
    Silvina Bartesaghi
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo 11800, Uruguay
    Biochemistry 45:6813-25. 2006
    ..BTBE was successfully applied as a hydrophobic probe to study nitration mechanisms and will serve to study factors controlling protein and lipid nitration in biomembranes and lipoproteins...
  55. ncbi request reprint Peroxynitrite-derived carbonate and nitrogen dioxide radicals readily react with lipoic and dihydrolipoic acid
    Madia Trujillo
    Departamento de Bioquimica, Facultad de Medicina, Universidad de la Republica, 11800 Montevideo, Uruguay
    Free Radic Biol Med 39:279-88. 2005
    ..The mechanisms of reaction defined herein allow to rationalize the biochemistry of peroxynitrite based on direct and free radical-mediated processes and contribute to the understanding of the antioxidant actions of LA and DHLA...
  56. ncbi request reprint Tyrosine nitration, dimerization, and hydroxylation by peroxynitrite in membranes as studied by the hydrophobic probe N-t-BOC-l-tyrosine tert-butyl ester
    Silvina Bartesaghi
    Department of Biochemistry, Facultad de Medicina Universidad de la República, Montevideo, Uruguay
    Methods Enzymol 441:217-36. 2008
    ..The methods presented herein can be extended to explore the reaction mechanisms of tyrosine oxidation by other biologically relevant oxidants and in other hydrophobic biocompartments...

Research Grants46

  1. Role of iNOS, Nitric Oxide & Arginase in Statin-Mediated Toxicity in Cancer Cells
    Balaraman Kalyanaraman; Fiscal Year: 2010
    ..Thus, it is both timely and important to understand the mechanism(s) by which statins kill breast cancer cells and to explore the possibility for clinical implementation of statins as chemopreventive drugs. ..
  2. Bicarbonate Enhances Peroxidation of SOD/ALS Mutants
    Balaraman Kalyanaraman; Fiscal Year: 2007
    ....
  3. Peroxide, NO and Iron Signaling in Endothelial Damage
    Balaraman Kalyanaraman; Fiscal Year: 2007
    ..Novelty: This proposal presents a new insight on peroxide-induced iron signaling in endothelial apoptosis and on the role of NO and antioxidants in mitigating these effects. ..
  4. ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINES
    Balaraman Kalyanaraman; Fiscal Year: 2006
    ..Understanding the relationship between DOX-induced apoptosis and ROS formation may help discover novel antioxidant/antiapoptotic therapy in oxidant-induced disease processes. ..
  5. NITRIC OXIDE MEDICATED OXIDATION/NITRATION
    Balaraman Kalyanaraman; Fiscal Year: 2006
    ..Novelty: This proposal will provide new insights on the role of hydrophobic membranes on nitration/oxidation/ nitrosation of membrane-associated proteins. ..
  6. Peroxide, NO and Iron Signaling in Endothelial Damage
    Balaraman Kalyanaraman; Fiscal Year: 2006
    ..Novelty: This proposal presents a new insight on peroxide-induced iron signaling in endothelial apoptosis and on the role of NO and antioxidants in mitigating these effects. ..
  7. Role of Neuronal NOS & Superoxide in Neurodegeneration
    Balaraman Kalyanaraman; Fiscal Year: 2006
    ..abstract_text> ..
  8. Bicarbonate Enhances Peroxidation of SOD/ALS Mutants
    Balaraman Kalyanaraman; Fiscal Year: 2006
    ....
  9. ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINES
    Balaraman Kalyanaraman; Fiscal Year: 2005
    ..Understanding the relationship between DOX-induced apoptosis and ROS formation may help discover novel antioxidant/antiapoptotic therapy in oxidant-induced disease processes. ..
  10. Bicarbonate Enhances Peroxidation of SOD/ALS Mutants
    Balaraman Kalyanaraman; Fiscal Year: 2005
    ....
  11. Role of Neuronal NOS & Superoxide in Neurodegeneration
    Balaraman Kalyanaraman; Fiscal Year: 2007
    ..abstract_text> ..
  12. Mitochondria-targeted Agents in Breast Cancer
    Balaraman Kalyanaraman; Fiscal Year: 2010
    ..This work will also enable early detection of breast cancer in an animal model using a novel imaging technique. ..
  13. Role of iNOS, Nitric Oxide & Arginase in Statin-Mediated Toxicity in Cancer Cells
    Balaraman Kalyanaraman; Fiscal Year: 2009
    ..Thus, it is both timely and important to understand the mechanism(s) by which statins kill breast cancer cells and to explore the possibility for clinical implementation of statins as chemopreventive drugs. ..
  14. Role of iNOS, Nitric Oxide & Arginase in Statin-Mediated Toxicity in Cancer Cells
    Balaraman Kalyanaraman; Fiscal Year: 2010
    ..Thus, it is both timely and important to understand the mechanism(s) by which statins kill breast cancer cells and to explore the possibility for clinical implementation of statins as chemopreventive drugs. ..
  15. Nitric Oxide-Mediated Oxidation/Nitration in Membranes
    Balaraman Kalyanaraman; Fiscal Year: 2010
    ..g., atherosclerosis, diabetes, Parkinson's and Lou Gehrig's). ..
  16. Neuroprotection by Mitochondria-targeted Antioxidants
    ANUMANTHA GOUNDER KANTHASAMY; Fiscal Year: 2010
    ....
  17. Neuroprotection by Mitochondria-targeted Antioxidants
    Balaraman Kalyanaraman; Fiscal Year: 2009
    ....
  18. Role of iNOS, Nitric Oxide & Arginase in Statin-Mediated Toxicity in Cancer Cells
    Balaraman Kalyanaraman; Fiscal Year: 2009
    ..Thus, it is both timely and important to understand the mechanism(s) by which statins kill breast cancer cells and to explore the possibility for clinical implementation of statins as chemopreventive drugs. ..
  19. Nitric Oxide-Mediated Oxidation/Nitration in Membranes
    Balaraman Kalyanaraman; Fiscal Year: 2009
    ..g., atherosclerosis, diabetes, Parkinson's and Lou Gehrig's). ..
  20. NITRIC OXIDE MEDICATED OXIDATION/NITRATION
    Balaraman Kalyanaraman; Fiscal Year: 2005
    ..Novelty: This proposal will provide new insights on the role of hydrophobic membranes on nitration/oxidation/ nitrosation of membrane-associated proteins. ..
  21. Peroxide, NO and Iron Signaling in Endothelial Damage
    Balaraman Kalyanaraman; Fiscal Year: 2005
    ..Novelty: This proposal presents a new insight on peroxide-induced iron signaling in endothelial apoptosis and on the role of NO and antioxidants in mitigating these effects. ..
  22. ROLE OF NEURONAL NOS AND SUPEROXIDE IN NEURODEGENERATION
    Balaraman Kalyanaraman; Fiscal Year: 2002
    ..The availability of novel superoxide dismutase mimetics has made it possible to prove more thoroughly regarding the nature of ROS causing the inactivation of aconitase in the intracellular milieu. ..
  23. NITRIC OXIDE MEDIATED OXIDATION/NITRATION IN MEMBRANES
    Balaraman Kalyanaraman; Fiscal Year: 2002
    ....
  24. BIOCARBONATE ENHANCES PEROXIDATION OF SOD/ALS MUTANTS
    Balaraman Kalyanaraman; Fiscal Year: 2001
    ..Four major analytical techniques to be used include electron spin resonance spectroscopy (ESR) and spin trapping; HPLC; UV-visible spectroscopy and chemiluminescence. ..
  25. ROLE OF NEURONAL NOS AND SUPEROXIDE IN NEURODEGENERATION
    Balaraman Kalyanaraman; Fiscal Year: 2001
    ..The availability of novel superoxide dismutase mimetics has made it possible to prove more thoroughly regarding the nature of ROS causing the inactivation of aconitase in the intracellular milieu. ..
  26. NITRIC OXIDE MEDIATED OXIDATION/NITRATION IN MEMBRANES
    Balaraman Kalyanaraman; Fiscal Year: 2001
    ....
  27. ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINES
    Balaraman Kalyanaraman; Fiscal Year: 2001
    ....
  28. NITRIC OXIDE MEDIATED OXIDATION/NITRATION IN MEMBRANES
    Balaraman Kalyanaraman; Fiscal Year: 2000
    ....
  29. ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINES
    Balaraman Kalyanaraman; Fiscal Year: 2000
    ....
  30. NITRIC OXIDE MEDIATED OXIDATION/NITRATION IN MEMBRANES
    Balaraman Kalyanaraman; Fiscal Year: 1999
    ....
  31. BIOCARBONATE ENHANCES PEROXIDATION OF SOD/ALS MUTANTS
    Balaraman Kalyanaraman; Fiscal Year: 2002
    ..Four major analytical techniques to be used include electron spin resonance spectroscopy (ESR) and spin trapping; HPLC; UV-visible spectroscopy and chemiluminescence. ..
  32. ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINES
    Balaraman Kalyanaraman; Fiscal Year: 2002
    ..Understanding the relationship between DOX-induced apoptosis and ROS formation may help discover novel antioxidant/antiapoptotic therapy in oxidant-induced disease processes. ..
  33. Peroxide, NO and Iron Signaling in Endothelial Damage
    Balaraman Kalyanaraman; Fiscal Year: 2003
    ..Novelty: This proposal presents a new insight on peroxide-induced iron signaling in endothelial apoptosis and on the role of NO and antioxidants in mitigating these effects. ..
  34. Role of Neuronal NOS & Superoxide in Neurodegeneration
    Balaraman Kalyanaraman; Fiscal Year: 2005
    ..abstract_text> ..
  35. ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINES
    Balaraman Kalyanaraman; Fiscal Year: 2004
    ..Understanding the relationship between DOX-induced apoptosis and ROS formation may help discover novel antioxidant/antiapoptotic therapy in oxidant-induced disease processes. ..
  36. NITRIC OXIDE MEDICATED OXIDATION/NITRATION
    Balaraman Kalyanaraman; Fiscal Year: 2004
    ..Novelty: This proposal will provide new insights on the role of hydrophobic membranes on nitration/oxidation/ nitrosation of membrane-associated proteins. ..
  37. Role of Neuronal NOS & Superoxide in Neurodegeneration
    Balaraman Kalyanaraman; Fiscal Year: 2004
    ..abstract_text> ..
  38. Peroxide, NO and Iron Signaling in Endothelial Damage
    Balaraman Kalyanaraman; Fiscal Year: 2004
    ..Novelty: This proposal presents a new insight on peroxide-induced iron signaling in endothelial apoptosis and on the role of NO and antioxidants in mitigating these effects. ..
  39. NITRIC OXIDE MEDICATED OXIDATION/NITRATION
    Balaraman Kalyanaraman; Fiscal Year: 2003
    ..Novelty: This proposal will provide new insights on the role of hydrophobic membranes on nitration/oxidation/ nitrosation of membrane-associated proteins. ..
  40. ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINES
    Balaraman Kalyanaraman; Fiscal Year: 2003
    ..Understanding the relationship between DOX-induced apoptosis and ROS formation may help discover novel antioxidant/antiapoptotic therapy in oxidant-induced disease processes. ..
  41. BIOCARBONATE ENHANCES PEROXIDATION OF SOD/ALS MUTANTS
    Balaraman Kalyanaraman; Fiscal Year: 2003
    ..Four major analytical techniques to be used include electron spin resonance spectroscopy (ESR) and spin trapping; HPLC; UV-visible spectroscopy and chemiluminescence. ..
  42. ROLE OF NEURONAL NOS AND SUPEROXIDE IN NEURODEGENERATION
    Balaraman Kalyanaraman; Fiscal Year: 2003
    ..The availability of novel superoxide dismutase mimetics has made it possible to prove more thoroughly regarding the nature of ROS causing the inactivation of aconitase in the intracellular milieu. ..
  43. ENOS & THE RADICAL MECHANISM OF ANTITUMOR ANTHRACYCLINES
    Balaraman Kalyanaraman; Fiscal Year: 1999
    ....