Howard Jacob

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. doi request reprint Genomics in clinical practice: lessons from the front lines
    Howard J Jacob
    Human and Molecular Genetic Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Sci Transl Med 5:194cm5. 2013
  2. pmc Characterization of human plasma-derived exosomal RNAs by deep sequencing
    Xiaoyi Huang
    Department of Pathology and Cancer Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    BMC Genomics 14:319. 2013
  3. pmc Rab38 modulates proteinuria in model of hypertension-associated renal disease
    Artur Rangel-Filho
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Am Soc Nephrol 24:283-92. 2013
  4. doi request reprint Identification of hypertension susceptibility loci on rat chromosome 12
    Michael J Flister
    Human and Molecular Genetics Center, Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Hypertension 60:942-8. 2012
  5. pmc A 4.1-Mb congenic region of Rf-4 contributes to glomerular permeability
    Caitlin C O'Meara
    Human and Molecular Genetics Center, Milwaukee, WI, USA
    J Am Soc Nephrol 23:825-33. 2012
  6. pmc Comprehensive quality control utilizing the prehybridization third-dye image leads to accurate gene expression measurements by cDNA microarrays
    Xujing Wang
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, The Medical College of Wisconsin and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    BMC Bioinformatics 7:378. 2006
  7. ncbi request reprint Rat genetics: attaching physiology and pharmacology to the genome
    Howard J Jacob
    Department of Physiology, Human and Molecular Genetics Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
    Nat Rev Genet 3:33-42. 2002
  8. pmc Gene targeting in the rat: advances and opportunities
    Howard J Jacob
    Department of Dermatology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Trends Genet 26:510-8. 2010
  9. pmc Dynamic convergence and divergence of renal genomic and biological pathways in protection from Dahl salt-sensitive hypertension
    Limin Lu
    Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Physiol Genomics 41:63-70. 2010
  10. ncbi request reprint Multiple blood pressure loci on rat chromosome 13 attenuate development of hypertension in the Dahl S hypertensive rat
    Carol Moreno
    Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53266, USA
    Physiol Genomics 31:228-35. 2007

Research Grants

  1. Physiological Genomics of Hypertensive Renal Disease
    Howard Jacob; Fiscal Year: 2007
  2. Knock-Out Rats for Physiological Genomics
    Howard Jacob; Fiscal Year: 2007
  3. RAT GENOME DATABASE
    Howard Jacob; Fiscal Year: 2007
  4. Physiological Genomics of Hypertensive Renal Disease
    Howard J Jacob; Fiscal Year: 2010
  5. RAT GENOME DATABASE
    Howard J Jacob; Fiscal Year: 2010
  6. Physioligical Genomics of Hypertensive Renal Disease
    Howard Jacob; Fiscal Year: 2004
  7. RAT GENOME DATABASE
    Howard Jacob; Fiscal Year: 2004
  8. CONSTRUCTION OF UNIGEN STYLE RAT MAP USING RAT RHS
    Howard Jacob; Fiscal Year: 2001
  9. RAT GENOME DATABASE
    Howard Jacob; Fiscal Year: 2001
  10. GENETIC DETERMINATES OF HIGH BLOOD PRESSURE
    Howard Jacob; Fiscal Year: 2004

Detail Information

Publications81

  1. doi request reprint Genomics in clinical practice: lessons from the front lines
    Howard J Jacob
    Human and Molecular Genetic Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Sci Transl Med 5:194cm5. 2013
    ..Here, we outline the lessons we have learned in converting a sequencing laboratory designed for research into a fully functional clinical program. ..
  2. pmc Characterization of human plasma-derived exosomal RNAs by deep sequencing
    Xiaoyi Huang
    Department of Pathology and Cancer Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    BMC Genomics 14:319. 2013
    ..In all we evaluated 14 libraries (7 replicates)...
  3. pmc Rab38 modulates proteinuria in model of hypertension-associated renal disease
    Artur Rangel-Filho
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Am Soc Nephrol 24:283-92. 2013
    ..Furthermore, our data suggest that Rab38 affects urinary protein excretion via effects in the proximal tubule...
  4. doi request reprint Identification of hypertension susceptibility loci on rat chromosome 12
    Michael J Flister
    Human and Molecular Genetics Center, Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Hypertension 60:942-8. 2012
    ....
  5. pmc A 4.1-Mb congenic region of Rf-4 contributes to glomerular permeability
    Caitlin C O'Meara
    Human and Molecular Genetics Center, Milwaukee, WI, USA
    J Am Soc Nephrol 23:825-33. 2012
    ....
  6. pmc Comprehensive quality control utilizing the prehybridization third-dye image leads to accurate gene expression measurements by cDNA microarrays
    Xujing Wang
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, The Medical College of Wisconsin and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    BMC Bioinformatics 7:378. 2006
    ..Previously, we have reported a novel 3-color microarray technology that enabled array fabrication QC. In this report we further investigated its advantage in spot-level data QC...
  7. ncbi request reprint Rat genetics: attaching physiology and pharmacology to the genome
    Howard J Jacob
    Department of Physiology, Human and Molecular Genetics Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
    Nat Rev Genet 3:33-42. 2002
    ..Most importantly, this combined physiological and genomic information should also lead to the development of better pre-clinical models of human disease, which will aid in the development of new therapeutics...
  8. pmc Gene targeting in the rat: advances and opportunities
    Howard J Jacob
    Department of Dermatology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Trends Genet 26:510-8. 2010
    ..Here, we provide a high-level description and the potential uses of these new technologies for investigators using the rat for biomedical research...
  9. pmc Dynamic convergence and divergence of renal genomic and biological pathways in protection from Dahl salt-sensitive hypertension
    Limin Lu
    Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Physiol Genomics 41:63-70. 2010
    ....
  10. ncbi request reprint Multiple blood pressure loci on rat chromosome 13 attenuate development of hypertension in the Dahl S hypertensive rat
    Carol Moreno
    Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53266, USA
    Physiol Genomics 31:228-35. 2007
    ..We conclude from these studies that there are four regions within chromosome 13 containing genes that interact epistatically and influence arterial pressure...
  11. ncbi request reprint Genomic map of cardiovascular phenotypes of hypertension in female Dahl S rats
    Carol Moreno
    Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 0509, USA
    Physiol Genomics 15:243-57. 2003
    ..The development of a female rat systems biology map of cardiovascular function represents the first attempt to prioritize those regions of the genome important for development of hypertension and end organ damage in female rats...
  12. pmc The Rat Genome Database (RGD): developments towards a phenome database
    Norberto de la Cruz
    Human and Molecular Genetics Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53213, USA
    Nucleic Acids Res 33:D485-91. 2005
    ....
  13. ncbi request reprint RF-2 gene modulates proteinuria and albuminuria independently of changes in glomerular permeability in the fawn-hooded hypertensive rat
    Artur Rangel-Filho
    Human and Molecular Genetics Center, Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Am Soc Nephrol 16:852-6. 2005
  14. pmc Transfer of the CYP4A region of chromosome 5 from Lewis to Dahl S rats attenuates renal injury
    Jan Michael Williams
    Kidney Disease Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Am J Physiol Renal Physiol 295:F1764-77. 2008
    ..These results indicate that the introgression of the CYP4A genes from Lewis rats into the Dahl S rats increases the renal formation of 20-HETE and attenuates the development of hypertension and renal disease...
  15. ncbi request reprint Mapping the genetic determinants of hypertension, metabolic diseases, and related phenotypes in the lyon hypertensive rat
    Marijo Bilusic
    Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Hypertension 44:695-701. 2004
    ..quot; These intermediate phenotypes may be used as closer surrogates to the mechanisms leading to hypertension and metabolic dysfunction in the LH rat...
  16. pmc RGD: a comparative genomics platform
    Mary Shimoyama
    Rat Genome Database, Human and Molecular Genetics Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Hum Genomics 5:124-9. 2011
    ..RGD houses gene, QTL and polymorphic marker data for rat, mouse and human and provides easy access to data through sophisticated searches, disease portals, interactive pathway diagrams and rat and human genome browsers...
  17. ncbi request reprint Consomic strategies to localize genomic regions related to vascular reactivity in the Dahl salt-sensitive rat
    Mary Pat Kunert
    University of Wisconsin Milwaukee, College of Nursing, Milwaukee, Wisconsin 53211, USA
    Physiol Genomics 26:218-25. 2006
    ..Because the BN chromosome is removed from its native background and the SS rat loses a native chromosome, it is also necessary to consider the contribution of changes in gene-to-gene interaction...
  18. doi request reprint Distinct genetic regulation of progression of diabetes and renal disease in the Goto-Kakizaki rat
    Marcelo A Nobrega
    Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53266, USA
    Physiol Genomics 39:38-46. 2009
    ..This observation would suggest that clinical studies collecting participants over a wide age distribution may be diluting genetic effects and reducing power to detect true effects...
  19. pmc Genetic mutation of recombination activating gene 1 in Dahl salt-sensitive rats attenuates hypertension and renal damage
    David L Mattson
    Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Am J Physiol Regul Integr Comp Physiol 304:R407-14. 2013
    ..These studies demonstrate the importance of renal infiltration of immune cells in the pathogenesis of hypertension and renal damage in Dahl SS rats...
  20. ncbi request reprint Genetic aspects of the etiology and treatment of asthma
    John R Meurer
    Medical College of Wisconsin and Children s Research Institute, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Pediatr Clin North Am 53:715-25. 2006
    ..Within a few years, practitioners may apply sophisticated knowledge of cell and molecular biology to expand pharmacotherapeutic approaches and to personalize diagnosis and management...
  21. ncbi request reprint Impact of genomics on research in the rat
    Jozef Lazar
    Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Genome Res 15:1717-28. 2005
    ..The impact of the rat genome project has just started, but it is an exciting time with tremendous progress...
  22. ncbi request reprint Characterization of blood pressure and renal function in chromosome 5 congenic strains of Dahl S rats
    Richard J Roman
    Dept of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Renal Physiol 290:F1463-71. 2006
    ....
  23. pmc The Rat Genome Database, update 2007--easing the path from disease to data and back again
    Simon N Twigger
    Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Nucleic Acids Res 35:D658-62. 2007
    ..We have published a number of papers that provide additional information on the ontology annotations and the tools at RGD for data mining and analysis to better enable researchers to fully utilize the database...
  24. ncbi request reprint BN phenome: detailed characterization of the cardiovascular, renal, and pulmonary systems of the sequenced rat
    Anne E Kwitek
    Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Physiol Genomics 25:303-13. 2006
    ..These data provide a baseline for physiological comparison of traits related to cardiovascular, lung, blood, and renal function in the sequenced BN rats relative to the major strains of rats studied in biomedical research...
  25. ncbi request reprint Efficient transgenic rat production by a lentiviral vector
    Mieczyslaw Michalkiewicz
    Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 293:H881-94. 2007
    ..Thus lentiviral transgenesis provides a powerful tool for the production of transgenic inbred rats and will enhance the usefulness of this species in gene discovery and target validation studies...
  26. pmc The Rat Genome Database curation tool suite: a set of optimized software tools enabling efficient acquisition, organization, and presentation of biological data
    Stanley J F Laulederkind
    Human and Molecular Genetics Center, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226 3548, USA
    Database (Oxford) 2011:bar002. 2011
    ..The user interfaces have been adapted to the work routines of the curators, creating a suite of tools that is intuitive and powerful. Database URL: http://rgd.mcw.edu...
  27. doi request reprint Making a definitive diagnosis: successful clinical application of whole exome sequencing in a child with intractable inflammatory bowel disease
    Elizabeth A Worthey
    Human and Molecular Genetics Center, The Medical College of Wisconsin, Milwaukee 53226, USA
    Genet Med 13:255-62. 2011
    ..The age and severity of the presentation suggested an underlying immune defect; however, despite comprehensive clinical evaluation, we were unable to arrive at a definitive diagnosis, thereby restricting clinical management...
  28. doi request reprint Genetic mapping of mammary tumor traits to rat chromosome 10 using a novel panel of consomic rats
    Tatjana Adamovic
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226 0509, USA
    Cancer Genet Cytogenet 186:41-8. 2008
    ..This novel approach should accelerate positional cloning of mammary cancer susceptibility and resistant genes in the rat and the identification of homologous genes in humans...
  29. pmc The Rat Genome Database 2009: variation, ontologies and pathways
    Melinda R Dwinell
    Department of Physiology and Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI, USA
    Nucleic Acids Res 37:D744-9. 2009
    ..By curating, storing, integrating, visualizing and promoting rat data, RGD ensures that the investment made into rat genomics and genetics can be leveraged by all interested investigators...
  30. pmc Chromosome substitution reveals the genetic basis of Dahl salt-sensitive hypertension and renal disease
    David L Mattson
    Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Am J Physiol Renal Physiol 295:F837-42. 2008
    ..Furthermore, this consomic rat panel provides a stable genetic platform that can facilitate further gene mapping by either linkage studies or the breeding of congenic and subcongenic rats...
  31. pmc What everybody should know about the rat genome and its online resources
    Simon N Twigger
    Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
    Nat Genet 40:523-7. 2008
    ....
  32. doi request reprint Molecular networks in Dahl salt-sensitive hypertension based on transcriptome analysis of a panel of consomic rats
    Mingyu Liang
    Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Physiol Genomics 34:54-64. 2008
    ....
  33. ncbi request reprint Chromosomal mapping of the genetic basis of hypertension and renal disease in FHH rats
    David L Mattson
    Dept of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Am J Physiol Renal Physiol 293:F1905-14. 2007
    ..The present data indicate that genes that can modify l-NAME-induced hypertension and proteinuria are on chromosomes 1, 15, 16, 18, and 20...
  34. ncbi request reprint A locus on chromosome 10 influences C-reactive protein levels in two independent populations
    Ulrich Broeckel
    Department of Medicine, Medical College of Wisconsin, Milwaukee 53226, USA
    Hum Genet 122:95-102. 2007
    ..This provides targets for the identification of genes involved in the regulation of hsCRP and the development and progression of vascular disease, including stroke...
  35. ncbi request reprint Identification of a QTL on chromosome 1 for impaired autoregulation of RBF in fawn-hooded hypertensive rats
    Bernardo Lopez
    Dept of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Renal Physiol 290:F1213-21. 2006
    ....
  36. ncbi request reprint Increased resistance to myocardial ischemia in the Brown Norway vs. Dahl S rat: role of nitric oxide synthase and Hsp90
    Yang Shi
    Division of Pediatric Surgery, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Mol Cell Cardiol 38:625-35. 2005
    ..NO production and decreases uncoupled NOS3 activity. We conclude increased association of hsp90 with NOS3 is a major mechanism by which BN/Mcw hearts are more resistant to ischemia than SS/Mcw hearts...
  37. ncbi request reprint Influence of diet and genetics on hypertension and renal disease in Dahl salt-sensitive rats
    David L Mattson
    Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Physiol Genomics 16:194-203. 2004
    ..These data demonstrate a significant influence of diet composition on salt-induced hypertension and renal disease in the Dahl SS/Mcw rat...
  38. ncbi request reprint Genetic mapping and characterization of the bleeding disorder in the fawn-hooded hypertensive rat
    Yvonne H Datta
    Department of Medicine, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Thromb Haemost 89:1031-42. 2003
    ..Therefore positional cloning of the gene responsible for the bleeding disorder in the FHH rat will lead to new insights in platelet physiology, with implications for diagnosis and management of hemostatic and thrombotic disorders...
  39. pmc Integrative genomics: in silico coupling of rat physiology and complex traits with mouse and human data
    Simon N Twigger
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Genome Res 14:651-60. 2004
    ..Integrating rat physiology with mouse genetics and clinical results from human by using the respective genomes provides a novel route to capitalize on comparative genomics and the strengths of model organism biology...
  40. pmc High-density rat radiation hybrid maps containing over 24,000 SSLPs, genes, and ESTs provide a direct link to the rat genome sequence
    Anne E Kwitek
    Human and Molecular Genetics Center and Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Genome Res 14:750-7. 2004
    ..This new integrated RH map contains approximately 10 RH-mapped elements per Mb on the genome assembly, enabling the RH maps to serve as a scaffold for a variety of data visualization tools...
  41. ncbi request reprint Transgenic rescue demonstrates involvement of the Ian5 gene in T cell development in the rat
    Mieczyslaw Michalkiewicz
    Department of Physiology, Human Molecular and Genetics Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 0509, USA
    Physiol Genomics 19:228-32. 2004
    ..It also demonstrates that transgenic rescue in the rat is a practical and definitive method for revealing the function of a novel gene...
  42. pmc Use of a three-color cDNA microarray platform to measure and control support-bound probe for improved data quality and reproducibility
    Martin J Hessner
    The Max McGee National Research Center for Juvenile Diabetes, Department of Pediatrics, The Medical College of Wisconsin and Children s Hospital of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Nucleic Acids Res 31:e60. 2003
    ..Collectively, this strategy will improve microarray data and increase its utility as a sensitive screening tool...
  43. ncbi request reprint Substitution of chromosome 1 ameliorates L-NAME hypertension and renal disease in the fawn-hooded hypertensive rat
    David L Mattson
    Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Am J Physiol Renal Physiol 288:F1015-22. 2005
    ..Qualitatively similar results were obtained from female FHH, FHH-1BN, and BN rats. The present results indicate that genes contributing to l-NAME-induced hypertension and renal disease are found on chromosome 1 of the FHH rat...
  44. ncbi request reprint Renal medullary genes in salt-sensitive hypertension: a chromosomal substitution and cDNA microarray study
    Mingyu Liang
    Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Physiol Genomics 8:139-49. 2002
    ..These results should provide an informative source for designing future functional studies in salt-sensitive hypertension...
  45. pmc Application of chromosomal substitution techniques in gene-function discovery
    Allen W Cowley
    Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Physiol 554:46-55. 2004
    ..Consomic rat strains are proving to be a unique scientific resource that greatly extends our understanding of genes and complex normal and pathological function...
  46. pmc Comparative recombination rates in the rat, mouse, and human genomes
    Michael I Jensen-Seaman
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Genome Res 14:528-38. 2004
    ..This comparative approach provides additional insight into the causes and consequences of genomic heterogeneity in recombination...
  47. ncbi request reprint A comprehensive linkage analysis for myocardial infarction and its related risk factors
    Ulrich Broeckel
    Department of Cardiovascular Medicine, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Nat Genet 30:210-4. 2002
    ..Although some of these risk factors overlap with previously identified loci, none overlaps with the newly identified susceptibility locus for myocardial infarction and coronary artery disease...
  48. pmc Creation and characterization of a renin knockout rat
    Carol Moreno
    Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Hypertension 57:614-9. 2011
    ....
  49. ncbi request reprint Genome-wide scanning with SSLPs in the rat
    Carol Moreno
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, USA
    Methods Mol Med 108:131-8. 2005
    ..Automation in the fluorescent detection system and data tracking software for processing genotypes, contributes to the ability to genotype large number of samples rapidly and accurately...
  50. doi request reprint Comparative genomics for detecting human disease genes
    Carol Moreno
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Adv Genet 60:655-97. 2008
    ..In this chapter, the current state of comparative genomics and the available tools are discussed in the context of developing animal model systems that reflect the clinical picture...
  51. doi request reprint Generation of gene-specific mutated rats using zinc-finger nucleases
    Aron M Geurts
    Human and Molecular Genetics Center, Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA
    Methods Mol Biol 597:211-25. 2010
    ..This technology allows for the rapid, targeted modification of the rat genome...
  52. ncbi request reprint Implications of circadian gene expression in kidney, liver and the effects of fasting on pharmacogenomic studies
    Yasuhiro Kita
    Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53228, USA
    Pharmacogenetics 12:55-65. 2002
    ..It also identifies 18 new genes that should be investigated for a role in circadian rhythms in peripheral tissues...
  53. ncbi request reprint Sequence analysis of the complete mitochondrial DNA in 10 commonly used inbred rat strains
    Nancy E Schlick
    Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53226 0509, USA
    Am J Physiol Cell Physiol 291:C1183-92. 2006
    ..Moreover, these data provide information that may be useful as new animal models, which result in novel combinations of nuclear and mitochondrial genomes, are developed...
  54. ncbi request reprint Tools and strategies for physiological genomics: the Rat Genome Database
    Simon N Twigger
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Physiol Genomics 23:246-56. 2005
    ..We present an overview of the database with a focus on these unique computational tools and describe strategies for the use of these resources in the area of physiological genomics...
  55. ncbi request reprint Identification of hypertension-related QTLs in African American sib pairs
    Theodore A Kotchen
    Department of Medicine, Medical College of Wisconsin, Milwaukee 53226, USA
    Hypertension 40:634-9. 2002
    ..Focusing on hypertension-related phenotypes is a promising approach for identifying the genetic determinants of hypertension...
  56. pmc A joint association test for multiple SNPs in genetic case-control studies
    Tao Wang
    Division of Biostatistics, Department of Population Health, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 0509, USA
    Genet Epidemiol 33:151-63. 2009
    ..Our simulation results also reveal that the latent variable approach may have improved power under certain scenarios comparing with classical haplotype association methods...
  57. ncbi request reprint Dickkopf homologs in squamous mucosa of esophagitis patients are overexpressed compared with Barrett's patients and healthy controls
    Irshad Ali
    Dysphagia Institute, Division of Gastroenterology and Hepatology, Medical College of Milwaukee, Wisconsin 53226, USA
    Am J Gastroenterol 101:1437-48. 2006
    ..The aim of this study was to identify and compare gene expression differences between normal distal and proximal squamous esophageal mucosa (SM) in esophagitis patients with that of healthy controls and Barrett's patients...
  58. pmc Genetic linkage and association of the growth hormone secretagogue receptor (ghrelin receptor) gene in human obesity
    Andrea Baessler
    Department of Physiology, Human and Molecular Genetics Center, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Diabetes 54:259-67. 2005
    ....
  59. ncbi request reprint Initial characterization of a rat model of diabetic nephropathy
    Marcelo A Nobrega
    Department of Physiology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Diabetes 53:735-42. 2004
    ..These results indicate that the T2DN rat is a suitable model for investigating diabetic nephropathy. Here we report the initial genetic and physiological characterization of this new rat model of diabetic nephropathy...
  60. doi request reprint The rat: a model used in biomedical research
    Howard J Jacob
    Human and Molecular Genetics Center, Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA
    Methods Mol Biol 597:1-11. 2010
    ..This book focuses on a variety of methodologies that can be used in this remarkable model. This chapter sets the stage by providing a perspective on why the rat remains an important model in biomedical research...
  61. doi request reprint Sequencing of the rat genome and databases
    Elizabeth A Worthey
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI, USA
    Methods Mol Biol 597:33-53. 2010
    ..This chapter is designed to overview the existing sequence and its utility, as well as provide a glimpse of some of the databases and bioinformatic tools available to the investigator...
  62. pmc Rat Genome Database (RGD): mapping disease onto the genome
    Simon Twigger
    Bioinformatics Research Center and Human and Molecular Genetics Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Nucleic Acids Res 30:125-8. 2002
    ..Thus, RGD is not only a valuable resource for those working with the rat but also for researchers in other model organisms wishing to harness the existing genetic and physiological data available in the rat to complement their own work...
  63. doi request reprint Microalterations of inherently unstable genomic regions in rat mammary carcinomas as revealed by long oligonucleotide array-based comparative genomic hybridization
    Tatjana Adamovic
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Cancer Res 69:5159-67. 2009
    ....
  64. ncbi request reprint Expression of G-protein alpha subunit genes in the vestibular periphery of Rattus norvegicus and their chromosomal mapping
    Joseph A Cioffi
    Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Acta Otolaryngol 123:1027-34. 2003
    ..The purpose of this study was to better define the role of these proteins by examining their expression in the rat vestibular periphery and characterizing their chromosomal location...
  65. pmc Refined mapping of the renal failure RF-3 quantitative trait locus
    Caitlin C O'Meara
    Human and Molecular Genetics Center, Department of Physiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Am Soc Nephrol 22:518-25. 2011
    ..By combining congenic models and cross-species studies, we narrowed the list of candidate genes to a level that we could sequence the whole interval to further identify the causative gene in future studies...
  66. ncbi request reprint Radiation hybrid mapping of five muscarinic acetylcholine receptor subtype genes in Rattus norvegicus
    Jeffrey Tseng
    Department of Otolaryngology and Communication Sciences, Medical College of Wisconsin, 9200 W Wisconsin Ave, Milwaukee, WI 53226, USA
    Hear Res 174:86-92. 2002
    ..With the chromosomal location for each of these muscarinic subtypes known, it is now possible to develop congenic and consomic strains of rats that can be used to study the functions of each of these subtypes...
  67. ncbi request reprint Distinct heritable patterns of angiographic coronary artery disease in families with myocardial infarction
    Marcus Fischer
    Human and Molecular Genetics Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Circulation 111:855-62. 2005
    ....
  68. ncbi request reprint Tissue engineering: a new frontier in physiological genomics
    Matthew C Petersen
    Physiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Physiol Genomics 32:28-32. 2007
    ....
  69. ncbi request reprint Renal damage susceptibility and autoregulation in RF-1 and RF-5 congenic rats
    Sabine J Van Dijk
    Department of Pediatric Surgery, Erasmus Medical Center, Rotterdam, The Netherlands
    Nephron Exp Nephrol 101:e59-66. 2005
    ..Here we present data obtained in congenic rats to directly assess the role of the Rf-1 and Rf-5 QTLs...
  70. ncbi request reprint Interaction between Rf-1 and Rf-4 quantitative trait loci increases susceptibility to renal damage in double congenic rats
    Sabine J Van Dijk
    Department of Paediatric Surgery, Erasmus MC, Rotterdam, The Netherlands
    Kidney Int 68:2462-72. 2005
    ..To investigate the separate role of the Rf-4 QTL and its interaction with Rf-1, we generated a single congenic strain carrying Rf-4 and a double congenic carrying both Rf-1 and Rf-4...
  71. ncbi request reprint Transfer of the Rf-1 region from FHH onto the ACI background increases susceptibility to renal impairment
    Abraham P Provoost
    Department of Pediatric Surgery, Erasmus University, 3000 DR Rotterdam, The Netherlands
    Physiol Genomics 8:123-9. 2002
    ..These findings indicate that the Rf-1B region of the FHH rat contains at least one gene affecting the susceptibility to progressive renal failure, especially in the presence of an increase in blood pressure...
  72. pmc Lymphopenia in the BB rat model of type 1 diabetes is due to a mutation in a novel immune-associated nucleotide (Ian)-related gene
    Armand J MacMurray
    Robert H Williams Laboratory, Department of Medicine, University of Washington, Seattle, Washington 98195, USA
    Genome Res 12:1029-39. 2002
    ..The lymphopenia mutation may thus be useful not only to elucidate Type 1 diabetes, but also in the function of the Ian gene family as a whole...
  73. ncbi request reprint Physiogenomic resources for rat models of heart, lung and blood disorders
    Renae L Malek
    TREX, The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, Maryland 20850, USA
    Nat Genet 38:234-9. 2006
    ..We performed a variety of data-mining manipulations on the profiles and used complementary physiological data from the PhysGen resource to demonstrate how TREX can be used by the cardiovascular community for hypothesis generation...
  74. ncbi request reprint Absence of an interaction between the Rf-1 and Rf-5 QTLs influencing susceptibility to renal damage in rats
    Sabine J Van Dijk
    Department of Paediatric Surgery, Erasmus MC, Rotterdam, The Netherlands
    Nephron Exp Nephrol 104:e96-e102. 2006
    ..The present study aimed to determine the presence of such interaction between the Rf-1 and Rf-5 QTLs...
  75. ncbi request reprint Genetic dissection of lymphopenia from autoimmunity by introgression of mutated Ian5 gene onto the F344 rat
    Daniel H Moralejo
    Robert H Williams Laboratory, Department of Medicine, University of Washington, Seattle, WA 98195, USA
    J Autoimmun 21:315-24. 2003
    ..The congenic F344.lyp rat should prove useful to dissect the mechanisms by which the Ian5 frameshift mutation affects T cell selection, differentiation and maturation without organ-specific autoimmunity...
  76. ncbi request reprint The Collaborative Cross, a community resource for the genetic analysis of complex traits
    Gary A Churchill
    The Jackson Laboratory, 600 Main Street Bar Harbor, Maine 04609, USA
    Nat Genet 36:1133-7. 2004
    ..The Collaborative Cross will provide a common reference panel specifically designed for the integrative analysis of complex systems and will change the way we approach human health and disease...
  77. ncbi request reprint Genome sequence of the Brown Norway rat yields insights into mammalian evolution
    Richard A Gibbs
    Human Genome Sequencing Center, Department of Molecular and Human Genetics, Baylor College of Medicine, MS BCM226, One Baylor Plaza, Houston, Texas 77030, USA lt http www hgsc bcm tmc edu
    Nature 428:493-521. 2004
    ....
  78. doi request reprint Progress and prospects in rat genetics: a community view
    Timothy J Aitman
    Medical Research Council Clinical Sciences Centre and Imperial College London, Du Cane Road, London W12 0NN, UK
    Nat Genet 40:516-22. 2008
    ..Finally, we present a set of principles that justify continuing and strengthening genetic studies in the rat model, and further development of genomic infrastructure for rat research...
  79. ncbi request reprint Rat model of familial combined hyperlipidemia as a result of comparative mapping
    Takahiro Ueno
    Centre de recherche du Centre Hospitalier de l Universite de Montreal, Montreal, Quebec, Canada
    Physiol Genomics 17:38-47. 2004
    ..These data suggest the close relationship between dyslipidemia in HTG rats and human FCHL, establishing a novel animal model for exploration of pathophysiology and therapy based on genomic determinants...
  80. ncbi request reprint Resolving the composite trait of hypertension into its pharmacogenetic determinants by acute pharmacological modulation of blood pressure regulatory systems
    Takahiro Ueno
    Centre de Recherche, Centre Hospitalier de l Universite de Montreal Hotel Dieu, 3850 Rue Saint Urbain, Montreal, Quebec, Canada
    J Mol Med (Berl) 81:51-60. 2003
    ..This pharmocogenetic approach enabled us to determine the main causative acute BP regulatory systems and should lead to better selection of suitable antihypertensive drugs for individual patients...
  81. pmc Nomenclature of the rat Cyp genes and the problems of gene nomenclature in general [Nelson, D.R. (2005), 'Gene nomenclature by default, or BLASTing to Babel', Hum. Genomics Vol. 2, pp. 196-201]
    Göran Levan
    Hum Genomics 2:343; author reply 343-4. 2006

Research Grants27

  1. Physiological Genomics of Hypertensive Renal Disease
    Howard Jacob; Fiscal Year: 2007
    ..We are confident that we will either find the gene or narrow down the interval to a handful of genes when we complete the term of this grant. ..
  2. Knock-Out Rats for Physiological Genomics
    Howard Jacob; Fiscal Year: 2007
    ..abstract_text> ..
  3. RAT GENOME DATABASE
    Howard Jacob; Fiscal Year: 2007
    ..A visiting scientist program will support curation, analyses and integration of specialized biological datasets. ..
  4. Physiological Genomics of Hypertensive Renal Disease
    Howard J Jacob; Fiscal Year: 2010
    ..We are confident that we will either find the gene or narrow down the interval to a handful of genes when we complete the term of this grant. ..
  5. RAT GENOME DATABASE
    Howard J Jacob; Fiscal Year: 2010
    ....
  6. Physioligical Genomics of Hypertensive Renal Disease
    Howard Jacob; Fiscal Year: 2004
    ..Using affected/unaffected human sibpairs, we will use SNP and sequence analysis to determine if individuals exhibiting ESRD show a significant association with sequence variants in candidate genes. ..
  7. RAT GENOME DATABASE
    Howard Jacob; Fiscal Year: 2004
    ..These proposed activities allow us to better serve the rat research community's needs and provide the wider scientific community with the ability to find, analyze and exploit rat-based data to promote their research. ..
  8. CONSTRUCTION OF UNIGEN STYLE RAT MAP USING RAT RHS
    Howard Jacob; Fiscal Year: 2001
    ..The combined efforts (MCW and U. of Iowa) will result in more than 1/3 of all rat genes mapped and placed on the RH "backbone" map facilitating comparative genomics with sequence "hooks". ..
  9. RAT GENOME DATABASE
    Howard Jacob; Fiscal Year: 2001
    ..abstract_text> ..
  10. GENETIC DETERMINATES OF HIGH BLOOD PRESSURE
    Howard Jacob; Fiscal Year: 2004
    ..The resulting synthesis of ideas and amassed data permits rigorous hypothesis testing not otherwise possible and will hasten understanding of the previously elusive genetic variation responsible for disease risk. ..
  11. Genetic and Cellular Basis of Resistance/Sensitivity to Myocardial Ischemia
    Howard J Jacob; Fiscal Year: 2010
    ..The overall goal of this project is to identify genes and mechanisms involved in resistance to myocardial infarction. ..