N Hogg

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. ncbi request reprint The kinetics of S-transnitrosation--a reversible second-order reaction
    N Hogg
    Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, Wisconsin 53227, USA
    Anal Biochem 272:257-62. 1999
  2. ncbi request reprint The effect of cyst(e)ine on the auto-oxidation of homocysteine
    N Hogg
    Biophysics Research Institute, Medical College of Wisconsin, Milwaukee 53226, USA
    Free Radic Biol Med 27:28-33. 1999
  3. ncbi request reprint Biological chemistry and clinical potential of S-nitrosothiols
    N Hogg
    Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 28:1478-86. 2000
  4. ncbi request reprint Red meat and colon cancer: heme proteins and nitrite in the gut. A commentary on "diet-induced endogenous formation of nitroso compounds in the GI tract"
    Neil Hogg
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Free Radic Biol Med 43:1037-9. 2007
  5. pmc Detection of nitric oxide by electron paramagnetic resonance spectroscopy
    Neil Hogg
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 49:122-9. 2010
  6. ncbi request reprint The biochemistry and physiology of S-nitrosothiols
    Neil Hogg
    Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Annu Rev Pharmacol Toxicol 42:585-600. 2002
  7. ncbi request reprint Inhibition of oxidized low-density lipoprotein-induced apoptosis in endothelial cells by nitric oxide. Peroxyl radical scavenging as an antiapoptotic mechanism
    S Kotamraju
    Biophysics Research Institute and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 276:17316-23. 2001
  8. ncbi request reprint Immuno-spin trapping of hemoglobin and myoglobin radicals derived from nitrite-mediated oxidation
    Agnes Keszler
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 40:507-15. 2006
  9. ncbi request reprint Mixing artifacts from the bolus addition of nitric oxide to oxymyoglobin: implications for S-nitrosothiol formation
    Yanhong Zhang
    Biophysics Research Institute and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 32:1212-9. 2002
  10. ncbi request reprint Reaction of tetrahydrobiopterin with superoxide: EPR-kinetic analysis and characterization of the pteridine radical
    J Vasquez-Vivar
    Department of Pathology, Medical College of Wisconsin, Milwaukee, 53226, USA
    Free Radic Biol Med 31:975-85. 2001

Research Grants

Collaborators

Detail Information

Publications61

  1. ncbi request reprint The kinetics of S-transnitrosation--a reversible second-order reaction
    N Hogg
    Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, Wisconsin 53227, USA
    Anal Biochem 272:257-62. 1999
    ..In addition, methods are shown for calculating the equilibrium distribution of the nitrosyl function group in mixtures of up to three thiols...
  2. ncbi request reprint The effect of cyst(e)ine on the auto-oxidation of homocysteine
    N Hogg
    Biophysics Research Institute, Medical College of Wisconsin, Milwaukee 53226, USA
    Free Radic Biol Med 27:28-33. 1999
    ..It is proposed that the major role of homocysteine is to reduce cystine to cysteine, and that cysteine autoxidation is the mechanism by which thiol-dependent oxidative stress occurs...
  3. ncbi request reprint Biological chemistry and clinical potential of S-nitrosothiols
    N Hogg
    Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 28:1478-86. 2000
    ..Many of the biological functions that have been described for S-nitrosothiols have clinical correlates. This review describes the biological chemistry, biological actions, and clinical potential of these compounds...
  4. ncbi request reprint Red meat and colon cancer: heme proteins and nitrite in the gut. A commentary on "diet-induced endogenous formation of nitroso compounds in the GI tract"
    Neil Hogg
    Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Free Radic Biol Med 43:1037-9. 2007
  5. pmc Detection of nitric oxide by electron paramagnetic resonance spectroscopy
    Neil Hogg
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 49:122-9. 2010
    ..This review gives a basic introduction to EPR spectroscopy and discusses its uses to assess and quantify nitric oxide formation in biological systems...
  6. ncbi request reprint The biochemistry and physiology of S-nitrosothiols
    Neil Hogg
    Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Annu Rev Pharmacol Toxicol 42:585-600. 2002
    ....
  7. ncbi request reprint Inhibition of oxidized low-density lipoprotein-induced apoptosis in endothelial cells by nitric oxide. Peroxyl radical scavenging as an antiapoptotic mechanism
    S Kotamraju
    Biophysics Research Institute and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 276:17316-23. 2001
    ..We propose that cellular lipid peroxyl radicals or lipid hydroperoxides induce an apoptotic signaling cascade in endothelial cells exposed to oxLDL, and that *NO inhibits apoptosis by scavenging cellular lipid peroxyl radicals...
  8. ncbi request reprint Immuno-spin trapping of hemoglobin and myoglobin radicals derived from nitrite-mediated oxidation
    Agnes Keszler
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 40:507-15. 2006
    ..The immuno-spin trapping technique represents an important new development for the study of the range and extent of protein oxidation by free radicals and oxidants...
  9. ncbi request reprint Mixing artifacts from the bolus addition of nitric oxide to oxymyoglobin: implications for S-nitrosothiol formation
    Yanhong Zhang
    Biophysics Research Institute and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 32:1212-9. 2002
    ..Our results suggest that the presence of the NO/O(2) reaction is an artifact of bolus NO addition, and leads to the formation of nitrite, or GSNO in the presence of GSH...
  10. ncbi request reprint Reaction of tetrahydrobiopterin with superoxide: EPR-kinetic analysis and characterization of the pteridine radical
    J Vasquez-Vivar
    Department of Pathology, Medical College of Wisconsin, Milwaukee, 53226, USA
    Free Radic Biol Med 31:975-85. 2001
    ..In conclusion, it is likely that BH(4)-dependent inhibition of superoxide formation from eNOS is the mechanism that better explains the antioxidant effects of BH(4) in the vasculature...
  11. pmc Reaction between nitric oxide, glutathione, and oxygen in the presence and absence of protein: How are S-nitrosothiols formed?
    Agnes Keszler
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 48:55-64. 2010
    ....
  12. ncbi request reprint Delayed cardioprotection with isoflurane: role of reactive oxygen and nitrogen
    Yang Shi
    Division of Pediatric Surgery, Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 288:H175-84. 2005
    ..8% but not in 1.0% isoflurane-treated hearts. We conclude that isoflurane confers delayed cardioprotection in the adult rat, triggered by ROS and RNS...
  13. ncbi request reprint Pulmonary arterial endothelial cells affect the redox status of coenzyme Q0
    Said H Audi
    Department of Biomedical Engineering, Marquette University, Milwaukee, WI, USA
    Free Radic Biol Med 34:892-907. 2003
    ..In addition, once formed the QH2 undergoes a cyanide-sensitive oxidation by the cells. Thus, the actual rate of Q reduction by the cells is greater than the net QH2 output from the cells...
  14. ncbi request reprint Oxidation and nitrosylation of oxyhemoglobin by S-nitrosoglutathione via nitroxyl anion
    Netanya Y Spencer
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 35:1515-26. 2003
    ..Specifically, the reaction of nitroxyl with S-nitrosothiols to liberate nitric oxide and reduced thiol is proposed to be central to the reaction mechanism...
  15. ncbi request reprint Metabolism of S-nitrosoglutathione by endothelial cells
    H Zeng
    Biophysics Research Institute and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Am J Physiol Heart Circ Physiol 281:H432-9. 2001
    ..We conclude that the intracellular thiol pool causes the reduction of extracellular disulfides to thiols, which then directly reduce GSNO...
  16. pmc Nitrite confers protection against myocardial infarction: role of xanthine oxidoreductase, NADPH oxidase and K(ATP) channels
    John E Baker
    Division of Cardiothoracic Surgery, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Mol Cell Cardiol 43:437-44. 2007
    ..Inhibition of NADPH oxidase and K(ATP) channels abolished nitrite-induced cardioprotection. Nitrite but not nitrate protects against infarction by a mechanism involving xanthine oxidoreductase, NADPH oxidase and K(ATP) channels...
  17. pmc The reaction between nitrite and oxyhemoglobin: a mechanistic study
    Agnes Keszler
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Biol Chem 283:9615-22. 2008
    ..The consequences of uncoupling the roles of H(2)O(2) and NO(2) in the reaction mechanism for the in vivo reactivity of nitrite are discussed...
  18. pmc Requirement of transmembrane transport for S-nitrosocysteine-dependent modification of intracellular thiols
    Katarzyna A Broniowska
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 281:33835-41. 2006
    ....
  19. pmc Attenuation of lipopolysaccharide-induced oxidative stress and apoptosis in fetal pulmonary artery endothelial cells by hypoxia
    Venkatesh Sampath
    Department of Pediatrics, Medical College of Wisconsin, Milwaukee, USA
    Free Radic Biol Med 46:663-71. 2009
    ..We conclude that ambient oxygen concentration modulates the severity of LPS-mediated injury in FPAEC by regulating superoxide levels produced in response to LPS...
  20. ncbi request reprint Antioxidant defense responses to sleep loss and sleep recovery
    Carol A Everson
    Department of Neurology, Medical College of Wisconsin, VAMC, Milwaukee WI 53295, USA
    Am J Physiol Regul Integr Comp Physiol 288:R374-83. 2005
    ..The present results link uncompensated oxidative stress to health effects induced by sleep deprivation and provide evidence that restoration of antioxidant balance is a property of recovery sleep...
  21. ncbi request reprint Enhancement of nitric oxide release from nitrosyl hemoglobin and nitrosyl myoglobin by red/near infrared radiation: potential role in cardioprotection
    Nicole L Lohr
    Department of Anesthesiology, Medical College of Wisconsin, MEB 4245, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
    J Mol Cell Cardiol 47:256-63. 2009
    ..Thus, the photodissociation to NO and its synergistic effect with sodium nitrite may represent a noninvasive and site-specific means for increasing NO bioavailability...
  22. pmc Phagocyte migration and cellular stress induced in liver, lung, and intestine during sleep loss and sleep recovery
    Carol A Everson
    Department of Neurology, The Medical College of Wisconsin, Neurology Research 151, VAMC, 5000 West National Ave, Milwaukee, WI 53295, USA
    Am J Physiol Regul Integr Comp Physiol 295:R2067-74. 2008
    ..Recovery sleep was marked by increased lung heme oxygenase-1, increased intestinal MPO activity, and abnormally low corticosterone, suggesting ongoing reactive processes as a result of prior sleep deprivation...
  23. ncbi request reprint Transmembrane nitration of hydrophobic tyrosyl peptides. Localization, characterization, mechanism of nitration, and biological implications
    Hao Zhang
    Biophysics Research Institute and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 278:8969-78. 2003
    ..Concomitantly, an increase in lipid oxidation was detected. A mechanism involving (.)NO(2) radical is proposed for peroxynitrite and MPO/H(2)O(2)/NO(2)(-)-dependent transmembrane nitration reactions...
  24. ncbi request reprint S-nitrosohemoglobin: a biochemical perspective
    Yanhong Zhang
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Free Radic Biol Med 36:947-58. 2004
    ....
  25. ncbi request reprint Anesthetic preconditioning: triggering role of reactive oxygen and nitrogen species in isolated hearts
    Enis Novalija
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, 53226, USA
    Am J Physiol Heart Circ Physiol 283:H44-52. 2002
    ..Thus APC is initiated by ROS as shown by improved function, reduced infarct size, and reduced dityrosine on reperfusion; protective and ROS/RNS-reducing effect of APC were attenuated when bracketed by ROS scavengers or NO* inhibition...
  26. ncbi request reprint Ischemic preconditioning: triggering role of nitric oxide-derived oxidants in isolated hearts
    Enis Novalija
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, USA
    J Cardiovasc Pharmacol 42:593-600. 2003
    ..These data support the hypothesis that the formation of nitric oxide-derived oxidants during ischemic preconditioning is causally related to myocardial adaptation to reperfusion injury...
  27. ncbi request reprint Impact of pulmonary arterial endothelial cells on duroquinone redox status
    Marilyn P Merker
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI, USA
    Free Radic Biol Med 37:86-103. 2004
    ..These endothelial redox functions and their apposition to the vessel lumen are consistent with the pulmonary endothelium being an important site of DQ reduction to DQH2 observed in the lungs...
  28. ncbi request reprint Nitration and oxidation of a hydrophobic tyrosine probe by peroxynitrite in membranes: comparison with nitration and oxidation of tyrosine by peroxynitrite in aqueous solution
    H Zhang
    Biophysics Research Institute and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Biochemistry 40:7675-86. 2001
    ..This work provides a new perspective on selective nitration of membrane-incorporated tyrosine analogues...
  29. pmc Vascular dysfunction in a murine model of severe hemolysis
    Anne C Frei
    Blood Research Institute, Blood Center of Wisconsin, Milwaukee, Wisconsin, USA
    Blood 112:398-405. 2008
    ..Together, these data indicate that sph/sph mice with severe HS have increased plasma hemoglobin and NO scavenging capacity, likely contributing to aberrant vasoregulation and initiating oxidative damage...
  30. ncbi request reprint Electron paramagnetic resonance analysis of nitrosylhemoglobin in humans during NO inhalation
    Barbora Piknova
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 280:40583-8. 2005
    ..v) The half-time of HbNO in vivo is approximately 40 min...
  31. ncbi request reprint Role of S-nitrosothiol transport in the cardioprotective effects of S-nitrosocysteine in rat hearts
    Neil Hogg
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 43:1086-94. 2007
    ..This suggests that the protective effects of CysNO are mediated through the posttranslational modification of cellular proteins through an NO-independent transnitrosation mechanism...
  32. pmc Decreased S-nitrosation of peptide thiols in the membrane interior
    Hao Zhang
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 47:962-8. 2009
    ..These data suggest that thiols located deeply in the hydrophobic core of the membrane may be less likely to undergo S-nitrosation in the presence of ()NO/O(2)...
  33. pmc Differential mechanisms of inhibition of glyceraldehyde-3-phosphate dehydrogenase by S-nitrosothiols and NO in cellular and cell-free conditions
    Katarzyna A Broniowska
    Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 299:H1212-9. 2010
    ..Additionally, the differences observed between the cellular system and purified protein strongly imply that the intracellular environment dictates the mechanism of inhibition...
  34. pmc Activation and inhibition of soluble guanylyl cyclase by S-nitrosocysteine: involvement of amino acid transport system L
    Joseph A Riego
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 47:269-74. 2009
    ....
  35. ncbi request reprint Comparative investigation of superoxide trapping by cyclic nitrone spin traps: the use of singular value decomposition and multiple linear regression analysis
    Agnes Keszler
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 35:1149-57. 2003
    ..4 M(-1)s(-1)), DEPMPO (0.53 M(-1)s(-1)), and BMPO (0.24 M(-1)s(-1)) determined through competition with spontaneous dismutation of superoxide, at pH 7.4 and 20 degrees C...
  36. pmc The mechanism of transmembrane S-nitrosothiol transport
    Yanhong Zhang
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Proc Natl Acad Sci U S A 101:7891-6. 2004
    ....
  37. ncbi request reprint Characterization and application of the biotin-switch assay for the identification of S-nitrosated proteins
    Yanhong Zhang
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Free Radic Biol Med 38:874-81. 2005
    ....
  38. pmc Differential regulation of metabolism by nitric oxide and S-nitrosothiols in endothelial cells
    Anne R Diers
    Department of Biophysics, Redox Biology Program, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Am J Physiol Heart Circ Physiol 301:H803-12. 2011
    ..Moreover, these data provide insight into the role of NO and related compounds in vascular (patho)physiology...
  39. pmc In-gel detection of S-nitrosated proteins using fluorescence methods
    Nicholas J Kettenhofen
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI, USA
    Methods Enzymol 441:53-71. 2008
    ..Second, it replaces the biotin label with fluorescent cyanine dyes in order to directly observe the modified proteins in-gel and perform comparative studies using difference gel electrophoresis analysis in two dimensions...
  40. ncbi request reprint S-Nitrosothiols: cellular formation and transport
    Yanhong Zhang
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Free Radic Biol Med 38:831-8. 2005
    ....
  41. pmc Proteomic methods for analysis of S-nitrosation
    Nicholas J Kettenhofen
    Department of Biophysics and Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 851:152-9. 2007
    ..This review will discuss the major methods that have been used for the proteomic analysis of protein S-nitrosation and discuss the pros and cons of this methodology...
  42. ncbi request reprint How do red blood cells dilate blood vessels?--Reply
    Daniel B Kim-Shapiro
    Circ Res 95:e10. 2004
  43. ncbi request reprint Cell-free hemoglobin limits nitric oxide bioavailability in sickle-cell disease
    Christopher D Reiter
    Critical Care Medicine Department of the Warren G Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland, USA
    Nat Med 8:1383-9. 2002
    ..Decompartmentalization of hemoglobin and subsequent dioxygenation of nitric oxide may explain the vascular complications shared by acute and chronic hemolytic disorders...
  44. ncbi request reprint Concerted nitric oxide formation and release from the simultaneous reactions of nitrite with deoxy- and oxyhemoglobin
    Rozalina Grubina
    Vascular Medicine Branch, NHLBI, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 282:12916-27. 2007
    ....
  45. pmc Cytoprotective effects of nitrite during in vivo ischemia-reperfusion of the heart and liver
    Mark R Duranski
    Department of Physiology, Louisiana State University Health Sciences Center, Shreveport, 71130, USA
    J Clin Invest 115:1232-40. 2005
    ..These studies reveal an unexpected and novel therapy for diseases such as myocardial infarction, organ preservation and transplantation, and shock states...
  46. pmc Enzymatic function of hemoglobin as a nitrite reductase that produces NO under allosteric control
    Zhi Huang
    Vascular Therapeutics Section, Cardiovascular Branch, National Heart, Lung and Blood Institute, and Laboratory of Chemical Biology, National Institute of Diabetes, Digestive and Kidney Diseases, NIH, Bethesda, Maryland 20892 1662, USA
    J Clin Invest 115:2099-107. 2005
    ..These data suggest that the oxygen sensor for hypoxic vasodilation is determined by Hb oxygen saturation and quaternary structure and that the nitrite reductase activity of Hb generates NO gas under allosteric and pH control...
  47. pmc Biological activity of nitric oxide in the plasmatic compartment
    Xunde Wang
    Critical Care Medicine Department, Warren G Magnuson Clinical Center, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892 1662, USA
    Proc Natl Acad Sci U S A 101:11477-82. 2004
    ..The studies further suggest that alternative NO reaction end products in plasma, such as nitrite, N-nitrosamines, iron-nitrosyls, and nitrated lipids, should be evaluated in blood NO transport along the vasculature...
  48. ncbi request reprint Formation and stability of S-nitrosothiols in RAW 264.7 cells
    Yanhong Zhang
    Am J Physiol Lung Cell Mol Physiol 287:L467-74. 2004
    ..To achieve intracellular localization of the nitrosation reaction, mechanisms must be invoked that do not involve the formation of nitric oxide as an intermediate...
  49. ncbi request reprint Ceruloplasmin is a NO oxidase and nitrite synthase that determines endocrine NO homeostasis
    Sruti Shiva
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nat Chem Biol 2:486-93. 2006
    ....
  50. ncbi request reprint Transduction of NO-bioactivity by the red blood cell in sepsis: novel mechanisms of vasodilation during acute inflammatory disease
    Jack H Crawford
    Department of Pathology, University of Alabama at Birmingham, 901 19th St S, BMR II Rm 307, Birmingham, AL 35294, USA
    Blood 104:1375-82. 2004
    ..Specifically, the concept that RBCs directly mediate systemic hypotension through NO-dependent mechanisms is discussed...
  51. ncbi request reprint The reaction between nitrite and hemoglobin: the role of nitrite in hemoglobin-mediated hypoxic vasodilation
    Daniel B Kim-Shapiro
    Department of Physics, Wake Forest University, Winston Salem, NC, USA
    J Inorg Biochem 99:237-46. 2005
    ....
  52. ncbi request reprint Fatty acid transduction of nitric oxide signaling. Nitrolinoleic acid is a hydrophobically stabilized nitric oxide donor
    Francisco J Schopfer
    Department of Anesthesiology and Center for Free Radical Biology, University of Alabama at Birmingham, Alabama 35294, USA
    J Biol Chem 280:19289-97. 2005
    ....
  53. ncbi request reprint Hemoglobin mediated nitrite activation of soluble guanylyl cyclase
    Anne Jeffers
    Department of Physics, Wake Forest University, Winston Salem, NC, 27109 7507 USA
    Comp Biochem Physiol A Mol Integr Physiol 142:130-5. 2005
    ..Thus, an intermediate species must be formed by the nitrite/deoxyhemoglobin reaction that escapes the red cell and effects vasodilation...
  54. ncbi request reprint Catalytic generation of N2O3 by the concerted nitrite reductase and anhydrase activity of hemoglobin
    Swati Basu
    Department of Physics, Wake Forest University, 1834 Wake Forest Road, Winston Salem, North Carolina 27109, USA
    Nat Chem Biol 3:785-94. 2007
    ....
  55. ncbi request reprint Nitric oxide scavenging by red blood cells as a function of hematocrit and oxygenation
    Ivan Azarov
    Department of Physics, School of Medicine, Wake Forest University, Winston Salem, North Carolina 27109, USA
    J Biol Chem 280:39024-32. 2005
    ..These observations may have important physiological implications within the microvasculature and for pathophysiological disruption of nitric oxide homeostasis in diseases...
  56. ncbi request reprint Methodological vexation about thiol oxidation versus S-nitrosation -- a commentary on "An ascorbate-dependent artifact that interferes with the interpretation of the biotin-switch assay"
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 41:557-61. 2006
  57. ncbi request reprint The reaction between nitrite and deoxyhemoglobin. Reassessment of reaction kinetics and stoichiometry
    Kris T Huang
    Department of Biomedical Engineering, Wake Forest University School of Medicine, Winston Salem, North Carolina, USA
    J Biol Chem 280:31126-31. 2005
    ....
  58. ncbi request reprint Nitrite as a vascular endocrine nitric oxide reservoir that contributes to hypoxic signaling, cytoprotection, and vasodilation
    Mark T Gladwin
    Vascular Medicine Branch, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892 1454, USA
    Am J Physiol Heart Circ Physiol 291:H2026-35. 2006
    ..An expanded consideration of nitrite as a hypoxia-dependent intrinsic signaling molecule has opened up a new field of research and therapeutic opportunities for diseases associated with regional hypoxia and vasoconstriction...
  59. pmc Copper dependence of the biotin switch assay: modified assay for measuring cellular and blood nitrosated proteins
    Xunde Wang
    Pulmonary and Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA
    Free Radic Biol Med 44:1362-72. 2008
    ..The addition of copper to ascorbate allows for a simple assay modification that dramatically increases sensitivity while maintaining specificity...
  60. pmc Measurements of nitric oxide on the heme iron and beta-93 thiol of human hemoglobin during cycles of oxygenation and deoxygenation
    Xiuli Xu
    Department of Physics, Wake Forest University, Winston Salem, NC 27109, USA
    Proc Natl Acad Sci U S A 100:11303-8. 2003
    ..Our results do not support a role for allosterically controlled intramolecular transfer of nitric oxide in hemoglobin as a function of oxygen saturation...
  61. ncbi request reprint The emerging biology of the nitrite anion
    Mark T Gladwin
    Vascular Medicine Branch, National Heart, Lung, and Blood Institute, US National Institutes of Health, Bethesda, Maryland, USA
    Nat Chem Biol 1:308-14. 2005
    ..A recent two-day symposium at the US National Institutes of Health highlighted recent advances in the understanding of nitrite biochemistry, physiology and therapeutics...

Research Grants9

  1. Mechanism of Metabolism of S-Nitrosothiols
    Neil Hogg; Fiscal Year: 2002
    ..It is envisioned that this study will yield new insights into the roles played by these important compounds and aid pharmacological development of new and more selectively potent S- nitrosothiols. ..
  2. Mechanism of Metabolism of S-Nitrosothiols
    Neil Hogg; Fiscal Year: 2003
    ..It is envisioned that this study will yield new insights into the roles played by these important compounds and aid pharmacological development of new and more selectively potent S- nitrosothiols. ..
  3. Mechanism of Metabolism of S-Nitrosothiols
    Neil Hogg; Fiscal Year: 2004
    ..It is envisioned that this study will yield new insights into the roles played by these important compounds and aid pharmacological development of new and more selectively potent S- nitrosothiols. ..
  4. Mechanism of Metabolism of S-Nitrosothiols
    Neil Hogg; Fiscal Year: 2005
    ..It is envisioned that this study will yield new insights into the roles played by these important compounds and aid pharmacological development of new and more selectively potent S- nitrosothiols. ..
  5. SUMMER RESEARCH EXPERIENCES FOR UNDERGRADUATES
    Neil Hogg; Fiscal Year: 2006
    ..The success of the program will be closely monitored by follow-up questionnaires to both students and participating faculty. ..
  6. Mechanism of Metabolism of S-Nitrosothiols
    Neil Hogg; Fiscal Year: 2007
    ..Importantly, these studies will point to a role of RSNO formation not simply as 'carriers' of NO bioactivity but as a distinct bifurcation in NO signaling pathways. ..
  7. Mechanism of Metabolism of S-Nitrosothiols
    Neil Hogg; Fiscal Year: 2008
    ..Importantly, these studies will point to a role of RSNO formation not simply as 'carriers' of NO bioactivity but as a distinct bifurcation in NO signaling pathways. ..
  8. Mechanism of Metabolism of S-Nitrosothiols
    Neil Hogg; Fiscal Year: 2009
    ..Importantly, these studies will point to a role of RSNO formation not simply as 'carriers'of NO bioactivity but as a distinct bifurcation in NO signaling pathways. ..
  9. Mechanism of Metabolism of S-Nitrosothiols
    Neil Hogg; Fiscal Year: 2010
    ..Importantly, these studies will point to a role of RSNO formation not simply as 'carriers'of NO bioactivity but as a distinct bifurcation in NO signaling pathways. ..