Yusuf Awni Hannun

Summary

Affiliation: Medical University of South Carolina
Country: USA

Publications

  1. ncbi The Ceramide-centric universe of lipid-mediated cell regulation: stress encounters of the lipid kind
    Yusuf A Hannun
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 277:25847-50. 2002
  2. pmc Remodeling of cellular cytoskeleton by the acid sphingomyelinase/ceramide pathway
    Youssef H Zeidan
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    J Cell Biol 181:335-50. 2008
  3. pmc Coordination of the dynamics of yeast sphingolipid metabolism during the diauxic shift
    Fernando Alvarez-Vasquez
    Dept of Biostatistics, Bioinformatics and Epidemiology Medical University of South Carolina, Charleston, SC USA
    Theor Biol Med Model 4:42. 2007
  4. pmc Evaluation of bioactive sphingolipids in 4-HPR-resistant leukemia cells
    Aintzane Apraiz
    Department of Cell Biology and Histology, School of Medicine and Dentistry, University of the Basque Country, Barrio Sarriena s n, 48940 Leioa Bizkaia, Spain
    BMC Cancer 11:477. 2011
  5. pmc Many ceramides
    Yusuf A Hannun
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SouthCarolina 29425, USA
    J Biol Chem 286:27855-62. 2011
  6. doi Principles of bioactive lipid signalling: lessons from sphingolipids
    Yusuf A Hannun
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, Charleston, South Carolina 29425, USA
    Nat Rev Mol Cell Biol 9:139-50. 2008
  7. ncbi The coordination of prostaglandin E2 production by sphingosine-1-phosphate and ceramide-1-phosphate
    Benjamin J Pettus
    Department of Biochemistry and Molecular Biology, Room 501, Basic Science Building, Medical University of South Carolina, 173 Ashley Avenue, P O Box 250509, Charleston, SC 29425, USA
    Mol Pharmacol 68:330-5. 2005
  8. ncbi Lipid metabolism: ceramide transfer protein adds a new dimension
    Yusuf A Hannun
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Ave, Charleston, South Carolina 29425, USA
    Curr Biol 14:R163-5. 2004
  9. ncbi The structural requirements for ceramide activation of serine-threonine protein phosphatases
    Charles E Chalfant
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, and Ralph H Johnson Veterans Administration Mecial Center, Charleston, SC, USA
    J Lipid Res 45:496-506. 2004
  10. ncbi Ceramide in the eukaryotic stress response
    Y A Hannun
    Dept of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29495, USA
    Trends Cell Biol 10:73-80. 2000

Research Grants

Collaborators

Detail Information

Publications68

  1. ncbi The Ceramide-centric universe of lipid-mediated cell regulation: stress encounters of the lipid kind
    Yusuf A Hannun
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 277:25847-50. 2002
  2. pmc Remodeling of cellular cytoskeleton by the acid sphingomyelinase/ceramide pathway
    Youssef H Zeidan
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    J Cell Biol 181:335-50. 2008
    ..Collectively, these results highlight a novel tumor suppressor property for Cer and a function for ASMase in cisplatin-induced cytoskeletal remodeling...
  3. pmc Coordination of the dynamics of yeast sphingolipid metabolism during the diauxic shift
    Fernando Alvarez-Vasquez
    Dept of Biostatistics, Bioinformatics and Epidemiology Medical University of South Carolina, Charleston, SC USA
    Theor Biol Med Model 4:42. 2007
    ....
  4. pmc Evaluation of bioactive sphingolipids in 4-HPR-resistant leukemia cells
    Aintzane Apraiz
    Department of Cell Biology and Histology, School of Medicine and Dentistry, University of the Basque Country, Barrio Sarriena s n, 48940 Leioa Bizkaia, Spain
    BMC Cancer 11:477. 2011
    ..Because sphingolipid metabolism has been reported to be altered in drug-resistant tumor cells, we studied the implication of sphingolipids in acquired resistance to 4-HPR based on an acute lymphoblastic leukemia model...
  5. pmc Many ceramides
    Yusuf A Hannun
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SouthCarolina 29425, USA
    J Biol Chem 286:27855-62. 2011
    ..In this minireview, we describe the "many ceramides" paradigm, along with the rationale, supporting evidence, and implications for our understanding of bioactive sphingolipids and approaches for unraveling these pathways...
  6. doi Principles of bioactive lipid signalling: lessons from sphingolipids
    Yusuf A Hannun
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, Charleston, South Carolina 29425, USA
    Nat Rev Mol Cell Biol 9:139-50. 2008
    ..Deciphering the mechanisms of these varied cell functions necessitates an understanding of the complex pathways of sphingolipid metabolism and the mechanisms that regulate lipid generation and lipid action...
  7. ncbi The coordination of prostaglandin E2 production by sphingosine-1-phosphate and ceramide-1-phosphate
    Benjamin J Pettus
    Department of Biochemistry and Molecular Biology, Room 501, Basic Science Building, Medical University of South Carolina, 173 Ashley Avenue, P O Box 250509, Charleston, SC 29425, USA
    Mol Pharmacol 68:330-5. 2005
    ..Taken together, the results provide, for the first time, a mechanism that assures the coordinate expression and activation in time and space of COX-2 and cPLA(2), assuring maximal production of PGE(2)...
  8. ncbi Lipid metabolism: ceramide transfer protein adds a new dimension
    Yusuf A Hannun
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Ave, Charleston, South Carolina 29425, USA
    Curr Biol 14:R163-5. 2004
    ....
  9. ncbi The structural requirements for ceramide activation of serine-threonine protein phosphatases
    Charles E Chalfant
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, and Ralph H Johnson Veterans Administration Mecial Center, Charleston, SC, USA
    J Lipid Res 45:496-506. 2004
    ..The studies also begin to define features that transform ceramide analogs into inhibitors of CAPP...
  10. ncbi Ceramide in the eukaryotic stress response
    Y A Hannun
    Dept of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29495, USA
    Trends Cell Biol 10:73-80. 2000
    ..They do this by regulating enzymes involved in its metabolism. Ceramide modulates a number of biochemical and cellular responses to stress, including apoptosis, cell-cycle arrest and cell senescence...
  11. ncbi Enzymes of sphingolipid metabolism: from modular to integrative signaling
    Y A Hannun
    Department of Biochemistry and Molecular Biology, The Medical University of South Carolina, Charleston 29425, USA
    Biochemistry 40:4893-903. 2001
    ..This may be a general prototype for the involvement of metabolic pathways in cell regulation...
  12. ncbi Biochemical properties of mammalian neutral sphingomyelinase 2 and its role in sphingolipid metabolism
    Norma Marchesini
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston 29425, USA
    J Biol Chem 278:13775-83. 2003
    ..In conclusion, these results 1) show that nSMase2 is a structural gene for nSMase, 2) suggest that nSMase2 acts as a bona fide N-SMase in cells, and 3) implicate nSMase2 in the regulation of cell growth and cell signaling...
  13. ncbi Lysosomotropic acid ceramidase inhibitor induces apoptosis in prostate cancer cells
    David H Holman
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC 29403, USA
    Cancer Chemother Pharmacol 61:231-42. 2008
    ..Effects of treatment on cytotoxicity, sphingolipid content, and apoptotic markers were assessed...
  14. ncbi Role of human longevity assurance gene 1 and C18-ceramide in chemotherapy-induced cell death in human head and neck squamous cell carcinomas
    Can E Senkal
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA
    Mol Cancer Ther 6:712-22. 2007
    ..In conclusion, these data suggest an important role for LASS1/C(18)-ceramide in gemcitabine/doxorubicin-induced cell death via the activation of caspase-9/3 in HNSCC...
  15. ncbi Loss of sphingosine kinase-1 activates the intrinsic pathway of programmed cell death: modulation of sphingolipid levels and the induction of apoptosis
    Tarek A Taha
    Division of General Internal Medicine, Ralph H Johnson Veterans Administration Hospital, Charleston, South Carolina, USA
    FASEB J 20:482-4. 2006
    ..These studies for the first time implicate endogenous SK1 as an important survival enzyme in MCF-7 cells and link the biological consequences of knocking down the enzyme to its biochemical role as a regulator of sphingolipid metabolism...
  16. ncbi Down-regulation of sphingosine kinase-1 by DNA damage: dependence on proteases and p53
    Tarek A Taha
    Division of General Internal Medicine, Ralph H Johnson Veterans Affairs Medical Center, Charleston, SC 29401, USA
    J Biol Chem 279:20546-54. 2004
    ..These studies demonstrate that SK1 is down-regulated by genotoxic stress, and that basal SK1 function may be necessary for the maintenance of tumor cell growth...
  17. pmc Novel analogs of D-e-MAPP and B13. Part 1: synthesis and evaluation as potential anticancer agents
    Zdzislaw M Szulc
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, POB 250509, Charleston, SC 29425, USA
    Bioorg Med Chem 16:1015-31. 2008
    ..However, some active analogs of the d-e-MAPP family were selective against different types of cancer. Compounds LCL85, LCL120, LCL385, LCL284, and LCL204 were identified to be promising lead compounds for therapeutic development...
  18. pmc Acid ceramidase upregulation in prostate cancer cells confers resistance to radiation: AC inhibition, a potential radiosensitizer
    Ayman E M Mahdy
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Mol Ther 17:430-8. 2009
    ..An improved understanding of radiotherapy and the application of combination therapy achieved in this study offer new opportunities for the modulation of radiation effects in the treatment of cancer...
  19. ncbi Tumor necrosis factor induces the loss of sphingosine kinase-1 by a cathepsin B-dependent mechanism
    Tarek A Taha
    Division of General Internal Medicine, Ralph H Johnson Veterans Administration Hospital, Charleston, South Carolina 29401, USA
    J Biol Chem 280:17196-202. 2005
    ..Therefore, these studies show that SK1 down-regulation by TNF is dependent on the "lysosomal pathway" of apoptosis and specifically on cathepsin B, which functions as an SK1 protease in cells...
  20. ncbi De novo ceramide regulates the alternative splicing of caspase 9 and Bcl-x in A549 lung adenocarcinoma cells. Dependence on protein phosphatase-1
    Charles E Chalfant
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA
    J Biol Chem 277:12587-95. 2002
    ..This is the first report on the dynamic regulation of RNA splicing of members of the Bcl-2 and caspase families in response to regulators of apoptosis...
  21. ncbi Defects in cell growth regulation by C18:0-ceramide and longevity assurance gene 1 in human head and neck squamous cell carcinomas
    Serap Koybasi
    Department of Biochemistry, Ralph H Johnson Veterans Affairs Medical Center, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 279:44311-9. 2004
    ..In conclusion, this study demonstrates, for the first time, a biological role for LAG1 and C18-ceramide in the regulation of growth of HNSCC...
  22. ncbi Rapid shortening of telomere length in response to ceramide involves the inhibition of telomere binding activity of nuclear glyceraldehyde-3-phosphate dehydrogenase
    Kamala P Sundararaj
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 279:6152-62. 2004
    ....
  23. ncbi Ceramide kinase mediates cytokine- and calcium ionophore-induced arachidonic acid release
    Benjamin J Pettus
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 278:38206-13. 2003
    ..This study identifies previously unknown roles for ceramide kinase and its product, C-1-P, in AA release and production of eicosanoids and provides clues for potential new targets to block inflammatory responses...
  24. ncbi Selective substrate supply in the regulation of yeast de novo sphingolipid synthesis
    L Ashley Cowart
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 282:12330-40. 2007
    ..These findings may provide insights into the many systems in which de novo synthesis is increased in the absence of elevated in vitro SPT activity...
  25. pmc A role for sphingosine kinase 1 in dextran sulfate sodium-induced colitis
    Ashley J Snider
    Department of Medicine, Medical University of South Carolina, 114 Doughty St, MSC 779, Charleston, SC 29425, USA
    FASEB J 23:143-52. 2009
    ..Inhibition of SK1 may prove to be a valuable therapeutic target by inhibiting systemic and local inflammation in IBD...
  26. ncbi A novel role for protein kinase Cdelta-mediated phosphorylation of acid sphingomyelinase in UV light-induced mitochondrial injury
    Youssef H Zeidan
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    FASEB J 22:183-93. 2008
    ..Taken together, this study provides key molecular insights into activation of ASMase in response to UV light, the role of PKCdelta in this activation, and the role of ASMase in mediating apoptotic responses...
  27. pmc Differential effects of ceramide and sphingosine 1-phosphate on ERM phosphorylation: probing sphingolipid signaling at the outer plasma membrane
    Daniel Canals
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 285:32476-85. 2010
    ..The approach also defines a tool kit to probe sphingolipid signaling at the plasma membrane...
  28. ncbi Substrate specificity of rat brain ceramidase
    Samer El Bawab
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425, USA
    J Lipid Res 43:141-8. 2002
    ..alpha-Hydroxylated Cer demonstrated considerably higher affinity, indicating a preference of the enzyme to those Cer molecular species. These results disclose a very high specificity of nonlysosomal CDase for its substrate, Cer...
  29. pmc Acid beta-glucosidase 1 counteracts p38delta-dependent induction of interleukin-6: possible role for ceramide as an anti-inflammatory lipid
    Kazuyuki Kitatani
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    J Biol Chem 284:12979-88. 2009
    ..Furthermore, the p38delta isoform was identified as a novel and predominant target of ceramide signaling as well as a regulator of IL-6 biosynthesis...
  30. ncbi Activation of acid sphingomyelinase by protein kinase Cdelta-mediated phosphorylation
    Youssef H Zeidan
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 282:11549-61. 2007
    ..Taken together, these results establish a novel direct biochemical mechanism for ASMase activation in which PKCdelta serves as a key upstream kinase...
  31. pmc Involvement of acid beta-glucosidase 1 in the salvage pathway of ceramide formation
    Kazuyuki Kitatani
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    J Biol Chem 284:12972-8. 2009
    ..Collectively, GBA1 is identified as a novel source of regulated formation of ceramide, and PKCdelta is an upstream regulator of this pathway...
  32. ncbi Involvement of sphingolipids in apoptin-induced cell killing
    Xiang Liu
    Department of Microbiology and Immunology, Medical University of South Carolina, P O Box 250504, 173 Ashley Avenue, Charleston, SC 29425, USA
    Mol Ther 14:627-36. 2006
    ..01) in apoptosis over either treatment modality alone. Together, these data suggest that apoptin modulates ceramide/sphingolipid metabolism as part of its mechanism of action...
  33. pmc Confluence induced threonine41/serine45 phospho-beta-catenin dephosphorylation via ceramide-mediated activation of PP1cgamma
    Norma Marchesini
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 175 Ashley Ave, POB 250509, Charleston, SC 29425, USA
    Biochim Biophys Acta 1771:1418-28. 2007
    ..Finally, evidence is provided for a role for this pathway in regulating cell motility during confluence...
  34. ncbi Sphingosine kinase 1 (SPHK1) is induced by transforming growth factor-beta and mediates TIMP-1 up-regulation
    Masayoshi Yamanaka
    Division of Rheumatology and Immunology, Medical University of South Carolina, Charleston, SC 29725, USA
    J Biol Chem 279:53994-4001. 2004
    ..Together, these experiments demonstrate that SphK/S1P are important components of the TGF-beta signaling pathway involved in up-regulation of the TIMP-1 gene...
  35. pmc Golgi fragmentation is associated with ceramide-induced cellular effects
    Wei Hu
    Department of Medicine and Biochemistry, Medical University of South Carolina, Charleston, SC 28425, USA
    Mol Biol Cell 16:1555-67. 2005
    ..Together, these results suggest that sphingosine generated from hydrolysis of ceramide causes the GC disruption, leading to various cellular effects...
  36. ncbi Dihydrosphingosine 1-phosphate stimulates MMP1 gene expression via activation of ERK1/2-Ets1 pathway in human fibroblasts
    Shizhong Bu
    Division of Rheumatology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    FASEB J 20:184-6. 2006
    ..This is the first report of selective function of dhS1P as compared with the better studied S1P...
  37. ncbi Role for neutral sphingomyelinase-2 in tumor necrosis factor alpha-stimulated expression of vascular cell adhesion molecule-1 (VCAM) and intercellular adhesion molecule-1 (ICAM) in lung epithelial cells: p38 MAPK is an upstream regulator of nSMase2
    Christopher J Clarke
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 282:1384-96. 2007
    ..These data reveal p38 MAPK as an upstream regulator of nSMase2 and indicate a role for nSMase2 in pro-inflammatory responses induced by TNF-alpha as a regulator of adhesion proteins...
  38. ncbi The sphingosine kinase 1/sphingosine-1-phosphate pathway mediates COX-2 induction and PGE2 production in response to TNF-alpha
    Benjamin J Pettus
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    FASEB J 17:1411-21. 2003
    ..Based on these findings, this study indicates that SK1 and S1P could be implicated in pathological inflammatory disorders and cancer...
  39. doi Regulation of neutral sphingomyelinase-2 (nSMase2) by tumor necrosis factor-alpha involves protein kinase C-delta in lung epithelial cells
    Christopher J Clarke
    Department of Biochemistry and Molecular Biology, 173 Ashley Ave, Charleston, SC 29425, USA
    Mol Pharmacol 74:1022-32. 2008
    ..Taken together, these data implicate PKC-delta as a regulator of nSMase2 and, for the first time, identify nSMase2 as a point of cross-talk between the PKC and sphingolipid pathways...
  40. ncbi Role for mammalian neutral sphingomyelinase 2 in confluence-induced growth arrest of MCF7 cells
    Norma Marchesini
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 279:25101-11. 2004
    ..Hence, we demonstrate for the first time that nSMase2 functions as a growth suppressor in MCF7 cells, linking confluence to the G(0)/G(1) cell cycle check point...
  41. ncbi Acute activation of de novo sphingolipid biosynthesis upon heat shock causes an accumulation of ceramide and subsequent dephosphorylation of SR proteins
    Gary M Jenkins
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 277:42572-8. 2002
    ..Moreover, SR dephosphorylation is emerging as a specific downstream target of accumulation of newly made ceramides in mammalian cells...
  42. ncbi Inhibition of growth and telomerase activity by novel cationic ceramide analogs with high solubility in human head and neck squamous cell carcinoma cells
    Michael J Rossi
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, USA
    Otolaryngol Head Neck Surg 132:55-62. 2005
    ....
  43. ncbi Ceramide in apoptosis: an overview and current perspectives
    Benjamin J Pettus
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA
    Biochim Biophys Acta 1585:114-25. 2002
    ....
  44. pmc Downregulation of neutral ceramidase by gemcitabine: Implications for cell cycle regulation
    Bill X Wu
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, 29425, USA
    Biochim Biophys Acta 1791:730-9. 2009
    ..NCDase siRNA also induced Rb dephosphorylation. These data provide evidence for a novel mechanism of action for GMZ and highlight downregulation of NCDase as a critical step in GMZ-mediated ceramide elevation and cell cycle arrest...
  45. ncbi Role of acid ceramidase in resistance to FasL: therapeutic approaches based on acid ceramidase inhibitors and FasL gene therapy
    Saeed Elojeimy
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina, USA
    Mol Ther 15:1259-63. 2007
    ....
  46. ncbi Acid ceramidase but not acid sphingomyelinase is required for tumor necrosis factor-{alpha}-induced PGE2 production
    Youssef H Zeidan
    Department of Biochemistry, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 281:24695-703. 2006
    ..Taken together, these results define a novel role for AC in the TNFalpha/PGE(2) pathway. In addition, the results of this study warrant careful reconsideration of desipramine as a specific inhibitor for ASMase...
  47. ncbi Protein kinase C-induced activation of a ceramide/protein phosphatase 1 pathway leading to dephosphorylation of p38 MAPK
    Kazuyuki Kitatani
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 281:36793-802. 2006
    ....
  48. pmc Regulation of the sphingosine-recycling pathway for ceramide generation by oxidative stress, and its role in controlling c-Myc/Max function
    Iyad Sultan
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA
    Biochem J 393:513-21. 2006
    ..These data demonstrate that the sphingosine-recycling pathway for the generation of endogenous long-chain ceramide in response to exogenous C6-cer is regulated by ROS, and plays an important biological role in controlling c-Myc function...
  49. ncbi Sphingosine kinase: biochemical and cellular regulation and role in disease
    Tarek Assad Taha
    Department of Medicine, Medical University of South Carolina, USA
    J Biochem Mol Biol 39:113-31. 2006
    ..This review is geared at mechanisms of regulation of sphingosine kinase and the coming to light of its role in disease...
  50. ncbi Purification, characterization, and identification of a sphingomyelin synthase from Pseudomonas aeruginosa. PlcH is a multifunctional enzyme
    Chiara Luberto
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 278:32733-43. 2003
    ..This SM synthase did not have reverse transferase activity. In conclusion, the Pseudomonas PlcH also exerts SM synthase activity; therefore, for the first time, we have identified a structural gene for a SM synthase...
  51. pmc Hydroxyurea sensitivity reveals a role for ISC1 in the regulation of G2/M
    Nabil Matmati
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, South Carolina 29403, USA
    J Biol Chem 284:8241-6. 2009
    ..Taken together, these findings implicate Isc1p as an upstream regulator of Swe1p levels and stability and Cdc28-Tyr-19 phosphorylation, in effect signaling recovery from the effects of genotoxic stress and allowing G(2)/M progression...
  52. pmc Defective acid sphingomyelinase pathway with Pseudomonas aeruginosa infection in cystic fibrosis
    Hong Yu
    Department of Pediatrics, Medical University of South Carolina, 173 Ashley BSB 749, Charleston, SC 29403, USA
    Am J Respir Cell Mol Biol 41:367-75. 2009
    ..aeruginosa infection. This study demonstrates that the defective ASMase pathway in CF is a key contributor to the unabated IL-8 response with P. aeruginosa infection and to the compromised host response failing to eradicate bacteria...
  53. pmc Blood sphingolipidomics in healthy humans: impact of sample collection methodology
    Samar M Hammad
    Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    J Lipid Res 51:3074-87. 2010
    ..Per particle, VLDL contained the highest levels of SM, Cer, and S1P. HPLC-MS/MS should provide a tool for clinical testing of circulating bioactive sphingolipids in human blood...
  54. ncbi Ceramide, stress, and a "LAG" in aging
    Lina M Obeid
    Ralph H Johnson Veterans Affairs Medical Center, Department of Veteran Affairs, Charleston, SC 29401, USA
    Sci Aging Knowledge Environ 2003:PE27. 2003
    ..In this Perspective, we examine the evidence for these connections in yeast, Drosophila, and mammals, and speculate on their implications...
  55. ncbi Sphingosine kinase 1 is up-regulated in colon carcinogenesis
    Toshihiko Kawamori
    Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    FASEB J 20:386-8. 2006
    ..These results suggest that the SK1/S1P pathway may play an important role in colon carcinogenesis, in part, by regulating COX-2 expression and PGE2 production...
  56. pmc Antiapoptotic roles of ceramide-synthase-6-generated C16-ceramide via selective regulation of the ATF6/CHOP arm of ER-stress-response pathways
    Can E Senkal
    Hollings Cancer Center, 86 Jonathan Lucas Street, Charleston, SC 29425, USA
    FASEB J 24:296-308. 2010
    ..Thus, these data reveal an unexpected and novel prosurvival role of CerS6/C(16)-ceramide involved in the protection against ER-stress-induced apoptosis and induction of HNSCC tumor growth...
  57. pmc Roles and regulation of secretory and lysosomal acid sphingomyelinase
    Russell W Jenkins
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA
    Cell Signal 21:836-46. 2009
    ..How a single gene product has the capacity to function in these diverse settings, and the subsequent impact on downstream ceramide-mediated biology is the subject of this review...
  58. ncbi Mitochondria and ceramide: intertwined roles in regulation of apoptosis
    Helene Birbes
    Ralph H Johnson Veteran Administration, Medical University of South Carolina, 29425, USA
    Adv Enzyme Regul 42:113-29. 2002
  59. ncbi Biochemical mechanisms of the generation of endogenous long chain ceramide in response to exogenous short chain ceramide in the A549 human lung adenocarcinoma cell line. Role for endogenous ceramide in mediating the action of exogenous ceramide
    Besim Ogretmen
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA
    J Biol Chem 277:12960-9. 2002
    ....
  60. ncbi Simultaneous quantitative analysis of bioactive sphingolipids by high-performance liquid chromatography-tandem mass spectrometry
    Jacek Bielawski
    Lipidomics Core, Department of Biochemistry and Molecular Biology and Hollings Cancer Center, Medical University of South Carolina, Charleston, SC 29425, USA
    Methods 39:82-91. 2006
    ..This and related methodology are already defining unexpected specialization and specificity in the metabolism and function of distinct subspecies of individual bioactive SPLs...
  61. pmc Involvement of dihydroceramide desaturase in cell cycle progression in human neuroblastoma cells
    Jacqueline M Kraveka
    Division of Hematology Oncology, Department of Pediatrics, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    J Biol Chem 282:16718-28. 2007
    ..Tautomycin was also able to block the hypophosphorylation of the retinoblastoma protein observed upon fenretinide treatment. These findings suggest a novel biological function for dihydroceramides...
  62. ncbi Identification of a novel phosphatidic acid binding domain in protein phosphatase-1
    Jeffrey A Jones
    Molecular and Cellular Biology and Pathobiology Program, Medical University of South Carolina, Charleston, South Carolina 29425, USA
    Biochemistry 44:13235-45. 2005
    ..When these results were taken together with the known crystallographic structure of PP1, they identified a novel PA binding region on PP1c gamma that contains a unique loop-strand structural fold responsible for the interaction with PA...
  63. ncbi Resistance to TRAIL is associated with defects in ceramide signaling that can be overcome by exogenous C6-ceramide without requiring down-regulation of cellular FLICE inhibitory protein
    Christina Voelkel-Johnson
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, 29403, USA
    Mol Cancer Ther 4:1320-7. 2005
    ..Our results suggest that ceramide plays a role in promoting TRAIL-mediated apoptosis and that TRAIL-resistant cancers may benefit from combination therapy with ceramide or agents that enhance ceramide accumulation...
  64. ncbi Mechanism of inhibition of sequestration of protein kinase C alpha/betaII by ceramide. Roles of ceramide-activated protein phosphatases and phosphorylation/dephosphorylation of protein kinase C alpha/betaII on threonine 638/641
    Kazuyuki Kitatani
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA
    J Biol Chem 282:20647-56. 2007
    ....
  65. pmc Novel analogs of D-e-MAPP and B13. Part 2: signature effects on bioactive sphingolipids
    Alicja Bielawska
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, 173 Ashley Avenue, PO Box 250509, Charleston, SC 29425, USA
    Bioorg Med Chem 16:1032-45. 2008
    ..These novel analogs should be useful in cell-based studies as specific regulators of Cer-Sph-S1P inter-metabolism, in vitro enzymatic studies, and for therapeutic development...
  66. ncbi Biologically active sphingolipids in cancer pathogenesis and treatment
    Besim Ogretmen
    Department of Biochemistry and Molecular Biology, and Hollings Cancer Center, 173 Ashley Avenue, Charleston, South Carolina 29425, USA
    Nat Rev Cancer 4:604-16. 2004
  67. pmc Hyaluronan constitutively regulates activation of COX-2-mediated cell survival activity in intestinal epithelial and colon carcinoma cells
    Suniti Misra
    Department of Cell Biology and Anatomy, Medical University of South Carolina, Charleston, SC 29425, USA
    J Biol Chem 283:14335-44. 2008
    ..Defining hyaluronan-dependent cyclooxygenase-2/prostaglandin E(2)-associated signaling pathways will provide a platform for developing novel therapeutic approaches for colon cancer...
  68. ncbi Roles for sphingolipid biosynthesis in mediation of specific programs of the heat stress response determined through gene expression profiling
    L Ashley Cowart
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, 29403, USA
    J Biol Chem 278:30328-38. 2003
    ....

Research Grants49

  1. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Hannun; Fiscal Year: 2003
    ..These studies are beginning to define novel signaling pathways that regulate distinct sub-families of PKC, with important implications for the understanding of tumor promotion, apoptosis and the other critical events regulated by PKC. ..
  2. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Hannun; Fiscal Year: 2005
    ..abstract_text> ..
  3. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Hannun; Fiscal Year: 2002
    ..These studies are beginning to define novel signaling pathways that regulate distinct sub-families of PKC, with important implications for the understanding of tumor promotion, apoptosis and the other critical events regulated by PKC. ..
  4. Modeling and Analysis of Yeast Sphingolipid Metabolism
    Yusuf Hannun; Fiscal Year: 2004
    ..This model system could then serve as a conceptual and practical platform to extend the hypothesis to other lipid classes, other aspects of regulated metabolism, and eventually to mammalian metabolism. ..
  5. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Hannun; Fiscal Year: 2009
    ..abstract_text> ..
  6. CERAMIDE ACTIVATED PROTEIN PHOSPHATASES
    Yusuf Hannun; Fiscal Year: 2009
    ..These studies should generate significant insight into novel aspects of signal transduction, regulation of phosphatases, and the regulation of cancer cell growth. ..
  7. Modeling and Analysis of Roles of Yeast Sphingolipids
    Yusuf Awni Hannun; Fiscal Year: 2010
    ..abstract_text> ..
  8. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Hannun; Fiscal Year: 2006
    ..abstract_text> ..
  9. SPHINGOMYELIN TURNOVER AND CELLULAR REGULATION
    Yusuf Hannun; Fiscal Year: 2006
    ..These pathways are of direct significance to the biology of stress responses and specifically to cancer therapeutics. ..
  10. Modeling and Analysis of Roles of Yeast Sphingolipids
    Yusuf Hannun; Fiscal Year: 2006
    ..The proposal endeavors to use biochemical, molecular as well as mathematical approaches that allow a deeper understanding of the stress response at a semi-comprehensive level, commensurate with the post-genomic era of research. ..
  11. Modeling and Analysis of Yeast Sphingolipid Metabolism
    Yusuf Hannun; Fiscal Year: 2005
    ..This model system could then serve as a conceptual and practical platform to extend the hypothesis to other lipid classes, other aspects of regulated metabolism, and eventually to mammalian metabolism. ..
  12. CERAMIDE ACTIVATED PROTEIN PHOSPHATASES
    Yusuf Hannun; Fiscal Year: 2007
    ..These studies should generate significant insight into novel aspects of signal transduction, regulation of phosphatases, and the regulation of cancer cell growth. ..
  13. Sphingolipids in Cancer Therapy and Angiogenesis
    Yusuf Hannun; Fiscal Year: 2007
    ..The studies should also result in the identification of novel strategies for cancer therapeutics aimed at inducing cancer cell death and/or inhibition of tumor angiogenesis. ..
  14. Modeling and Analysis of Roles of Yeast Sphingolipids
    Yusuf Hannun; Fiscal Year: 2007
    ..The proposal endeavors to use biochemical, molecular as well as mathematical approaches that allow a deeper understanding of the stress response at a semi-comprehensive level, commensurate with the post-genomic era of research. ..
  15. Modeling and Analysis of Yeast Sphingolipid Metabolism
    Yusuf Hannun; Fiscal Year: 2001
    ..This model system could then serve as a conceptual and practical platform to extend the hypothesis to other lipid classes, other aspects of regulated metabolism, and eventually to mammalian metabolism. ..
  16. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Hannun; Fiscal Year: 2001
    ..These studies are beginning to define novel signaling pathways that regulate distinct sub-families of PKC, with important implications for the understanding of tumor promotion, apoptosis and the other critical events regulated by PKC. ..
  17. SPHINGOMYELIN TURNOVER AND CELLULAR REGULATION
    Yusuf Hannun; Fiscal Year: 2003
    ..These pathways are of direct significance to the biology of stress responses and specifically to cancer therapeutics. ..
  18. SPHINGOMYELIN TURNOVER AND CELLULAR REGULATION
    Yusuf Hannun; Fiscal Year: 1999
    ..N-SMase may emerge as a key target in the understanding of the mechanisms of the stress response and in therapeutic development. ..
  19. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Hannun; Fiscal Year: 2007
    ..abstract_text> ..
  20. SPHINGOMYELIN TURNOVER AND CELLULAR REGULATION
    Yusuf Hannun; Fiscal Year: 2007
    ..These pathways are of direct significance to the biology of stress responses and specifically to cancer biology and inflammation. ..
  21. CERAMIDE ACTIVATED PROTEIN PHOSPHATASES
    Yusuf Hannun; Fiscal Year: 2001
    ..These may have important implications in the study of cancer biology and therapeutics. ..
  22. SPHINGOMYELIN TURNOVER AND CELLULAR REGULATION
    Yusuf Hannun; Fiscal Year: 2001
    ..N-SMase may emerge as a key target in the understanding of the mechanisms of the stress response and in therapeutic development. ..
  23. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Hannun; Fiscal Year: 2000
    ..These studies are beginning to define novel signaling pathways that regulate distinct sub-families of PKC, with important implications for the understanding of tumor promotion, apoptosis and the other critical events regulated by PKC. ..
  24. LC/Q Mass Spectrometry for Lipid Analysis
    Yusuf Hannun; Fiscal Year: 2002
    ..4. Foster and further develop ongoing interdisciplinary research Among the various users. ..
  25. SPHINGOMYELIN TURNOVER AND CELLULAR REGULATION
    Yusuf Hannun; Fiscal Year: 2000
    ..N-SMase may emerge as a key target in the understanding of the mechanisms of the stress response and in therapeutic development. ..
  26. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Hannun; Fiscal Year: 2004
    ..abstract_text> ..
  27. CERAMIDE ACTIVATED PROTEIN PHOSPHATASES
    Yusuf Hannun; Fiscal Year: 2003
    ..These may have important implications in the study of cancer biology and therapeutics. ..
  28. CERAMIDE ACTIVATED PROTEIN PHOSPHATASES
    Yusuf Hannun; Fiscal Year: 2004
    ..These may have important implications in the study of cancer biology and therapeutics. ..
  29. Modeling and Analysis of Yeast Sphingolipid Metabolism
    Yusuf Hannun; Fiscal Year: 2002
    ..This model system could then serve as a conceptual and practical platform to extend the hypothesis to other lipid classes, other aspects of regulated metabolism, and eventually to mammalian metabolism. ..
  30. SPHINGOMYELIN TURNOVER AND CELLULAR REGULATION
    Yusuf Hannun; Fiscal Year: 2002
    ..N-SMase may emerge as a key target in the understanding of the mechanisms of the stress response and in therapeutic development. ..
  31. Modeling and Analysis of Roles of Yeast Sphingolipids
    Yusuf Awni Hannun; Fiscal Year: 2010
    ....
  32. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Awni Hannun; Fiscal Year: 2010
    ..We are defining the significance and mechanisms by which this process occurs and is regulated. In particular we focus on a key signaling protein known as protein kinase C (PKC) and its activating lipids. ..
  33. ROLE AND REGULATION OF PROTEIN KINASE C ISOENZYMES
    Yusuf Hannun; Fiscal Year: 1999
    ....
  34. Modeling and Analysis of Roles of Yeast Sphingolipids
    Yusuf Hannun; Fiscal Year: 2009
    ..abstract_text> ..
  35. Sphingolipids in Cancer Therapy and Angiogenesis
    Yusuf Hannun; Fiscal Year: 2007
    ..The studies should also result in the identification of novel strategies for cancer therapeutics aimed at inducing cancer cell death and/or inhibition of tumor angiogenesis. ..
  36. CERAMIDE ACTIVATED PROTEIN PHOSPHATASES
    Yusuf Hannun; Fiscal Year: 2002
    ..These may have important implications in the study of cancer biology and therapeutics. ..
  37. CERAMIDE ACTIVATED PROTEIN PHOSPHATASES
    Yusuf Hannun; Fiscal Year: 2006
    ..These studies should generate significant insight into novel aspects of signal transduction, regulation of phosphatases, and the regulation of cancer cell growth. ..
  38. Modeling and Analysis of Yeast Sphingolipid Metabolism
    Yusuf Hannun; Fiscal Year: 2003
    ..This model system could then serve as a conceptual and practical platform to extend the hypothesis to other lipid classes, other aspects of regulated metabolism, and eventually to mammalian metabolism. ..
  39. CERAMIDE ACTIVATED PROTEIN PHOSPHATASES
    Yusuf Hannun; Fiscal Year: 2005
    ..These studies should generate significant insight into novel aspects of signal transduction, regulation of phosphatases, and the regulation of cancer cell growth. ..