William Dynan

Summary

Affiliation: Medical College of Georgia
Country: USA

Publications

  1. ncbi Modifying the function of DNA repair nanomachines for therapeutic benefit
    William S Dynan
    Institute of Molecular Medicine and Genetics, Cancer Biology and Regulation Program, Medical College of Georgia, Augusta, GA 30912, USA
    Nanomedicine 2:74-81. 2006
  2. ncbi Understanding and re-engineering nucleoprotein machines to cure human disease
    William Dynan
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    Nanomedicine (Lond) 3:93-105. 2008
  3. ncbi Biological effects of high-energy neutrons measured in vivo using a vertebrate model
    Wendy W Kuhne
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912, USA
    Radiat Res 172:473-80. 2009
  4. ncbi Porous-wall hollow glass microspheres as novel potential nanocarriers for biomedical applications
    Shuyi Li
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912, USA
    Nanomedicine 6:127-36. 2010
  5. ncbi Identification of the polypyrimidine tract binding protein-associated splicing factor.p54(nrb) complex as a candidate DNA double-strand break rejoining factor
    Catherine L Bladen
    Program in Gene Regulation and Cancer Biology, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    J Biol Chem 280:5205-10. 2005
  6. ncbi Expression of the Ku70 subunit (XRCC6) and protection from low dose ionizing radiation during zebrafish embryogenesis
    Catherine L Bladen
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    Neurosci Lett 422:97-102. 2007
  7. ncbi Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a requirement for an Mre11/Rad50/NBS1-containing fraction
    Juren Huang
    Institute of Molecular Medicine and Genetics, Program in Gene Regulation, CB 2803, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912, USA
    Nucleic Acids Res 30:667-74. 2002
  8. ncbi DNA damage response and Ku80 function in the vertebrate embryo
    Catherine L Bladen
    Institute of Molecular Medicine and Genetics, Medical College of Georgia Augusta, GA 30912, USA
    Nucleic Acids Res 33:3002-10. 2005
  9. ncbi Modification of the ionizing radiation response in living cells by an scFv against the DNA-dependent protein kinase
    Shuyi Li
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    Nucleic Acids Res 31:5848-57. 2003
  10. ncbi Identification of DNA-PKcs phosphorylation sites in XRCC4 and effects of mutations at these sites on DNA end joining in a cell-free system
    Kyung Jong Lee
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Room CB 2803, Augusta, GA 30912, USA
    DNA Repair (Amst) 3:267-76. 2004

Research Grants

  1. Laser Capture Microscopy and 2D-DIGE: Cancer Proteomics
    William Dynan; Fiscal Year: 2007
  2. Regulation of DNA Double-Strand Break Repair
    William Dynan; Fiscal Year: 2007
  3. INTERACTIONS OF DNA-PK WITH THE TRANSCRIPTION APPARATUS
    William Dynan; Fiscal Year: 2004
  4. Investigation of a Novel Role for RNA Binding Proteins in DNA Repair
    William S Dynan; Fiscal Year: 2010

Collaborators

  • Shuyi Li
  • Jeffrey R Lee
  • Wendy W Kuhne
  • Gang Bao
  • Honglu Wu
  • Catherine L Bladen
  • Paul M Weinberger
  • Mark A Merkley
  • Durga Udayakumar
  • Robert H Podolsky
  • David J Kozlowski
  • Marko Jovanovic
  • Lana L Jackson
  • Olga Greengauz-Roberts
  • Kyung Jong Lee
  • Juren Huang
  • Xianming Mo
  • Amanda Psyrri
  • Sunny S Khichi
  • Melody A Flowers
  • Katsuya Miyake
  • Sammy Navarre
  • John T Barrett
  • Wai K Lam
  • James R Goldenring
  • Hirokazu Yamaguchi
  • Yoshihiko Takeda
  • Sachiyo Nomura
  • Kyung-Jong Lee
  • Farlyn Z Hudson

Detail Information

Publications18

  1. ncbi Modifying the function of DNA repair nanomachines for therapeutic benefit
    William S Dynan
    Institute of Molecular Medicine and Genetics, Cancer Biology and Regulation Program, Medical College of Georgia, Augusta, GA 30912, USA
    Nanomedicine 2:74-81. 2006
    ..Third, we discuss similarities in the overall size, shape, and design of different nanomachines that manipulate DNA and RNA, and the possibility of developing nanomachines with new specificities not found in nature...
  2. ncbi Understanding and re-engineering nucleoprotein machines to cure human disease
    William Dynan
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    Nanomedicine (Lond) 3:93-105. 2008
    ....
  3. ncbi Biological effects of high-energy neutrons measured in vivo using a vertebrate model
    Wendy W Kuhne
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912, USA
    Radiat Res 172:473-80. 2009
    ..3 Gy. Results of this experiment indicate that medaka are a useful model for investigating biological damage associated with high-energy neutron exposure...
  4. ncbi Porous-wall hollow glass microspheres as novel potential nanocarriers for biomedical applications
    Shuyi Li
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912, USA
    Nanomedicine 6:127-36. 2010
    ..PW-HGMs remained in place after mouse intratumoral injection, suggesting a possible application for the delivery of anti-cancer drugs...
  5. ncbi Identification of the polypyrimidine tract binding protein-associated splicing factor.p54(nrb) complex as a candidate DNA double-strand break rejoining factor
    Catherine L Bladen
    Program in Gene Regulation and Cancer Biology, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    J Biol Chem 280:5205-10. 2005
    ..Based on structural comparison with related proteins, we propose a model where the four RNA recognition motif domains in the heteromeric PSF.p54(nrb) complex cooperate to align separate DNA molecules...
  6. ncbi Expression of the Ku70 subunit (XRCC6) and protection from low dose ionizing radiation during zebrafish embryogenesis
    Catherine L Bladen
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    Neurosci Lett 422:97-102. 2007
    ..These results suggest that Ku70 protein plays a crucial role in protecting the developing nervous system from radiation-induced DNA damage during embryogenesis...
  7. ncbi Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a requirement for an Mre11/Rad50/NBS1-containing fraction
    Juren Huang
    Institute of Molecular Medicine and Genetics, Program in Gene Regulation, CB 2803, Medical College of Georgia, 1120 15th Street, Augusta, GA 30912, USA
    Nucleic Acids Res 30:667-74. 2002
    ....
  8. ncbi DNA damage response and Ku80 function in the vertebrate embryo
    Catherine L Bladen
    Institute of Molecular Medicine and Genetics, Medical College of Georgia Augusta, GA 30912, USA
    Nucleic Acids Res 33:3002-10. 2005
    ..Results suggest that nonhomologous end joining components mediate DNA repair to promote survival of irradiated cells during embryogenesis...
  9. ncbi Modification of the ionizing radiation response in living cells by an scFv against the DNA-dependent protein kinase
    Shuyi Li
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    Nucleic Acids Res 31:5848-57. 2003
    ..The ability to modify the radiation response in situ in living cells provides a link between biochemical, genetic and cytologic approaches to the study of double-strand break repair intermediates...
  10. ncbi Identification of DNA-PKcs phosphorylation sites in XRCC4 and effects of mutations at these sites on DNA end joining in a cell-free system
    Kyung Jong Lee
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Room CB 2803, Augusta, GA 30912, USA
    DNA Repair (Amst) 3:267-76. 2004
    ..Results demonstrate that DNA-PKcs-mediated phosphorylation of XRCC4 serine 260 and serine 318 does not directly control end-joining under the conditions tested...
  11. ncbi Quantification of ionizing radiation-induced cell death in situ in a vertebrate embryo
    Catherine L Bladen
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    Radiat Res 168:149-57. 2007
    ..Together, these results illustrate the potential of the zebrafish for quantitative studies of radiation-induced cell death during embryogenesis and in vivo...
  12. ncbi Terminal DNA structure and ATP influence binding parameters of the DNA-dependent protein kinase at an early step prior to DNA synapsis
    Marko Jovanovic
    Program in Cancer Biology and Gene Regulation, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    Nucleic Acids Res 34:1112-20. 2006
    ..ATP and a nonhydrolyzable ATP analog also influence the stability of the DNA-PKcs*DNA complex, apparently by an allosteric mechanism that does not require DNA-PKcs autophosphorylation...
  13. ncbi 2D-DIGE proteomic characterization of head and neck squamous cell carcinoma
    Mark A Merkley
    Institute for Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    Otolaryngol Head Neck Surg 141:626-32. 2009
    ..Identify proteins that are differentially expressed between head and neck squamous cell cancer (HNSCC) and patient-matched normal adjacent tissue, and validate findings in a separate patient cohort...
  14. ncbi Subnuclear localization of Ku protein: functional association with RNA polymerase II elongation sites
    Xianming Mo
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia 30912, USA
    Mol Cell Biol 22:8088-99. 2002
    ..Tethering of double-strand break repair proteins to defined subnuclear structures may also be advantageous in maintenance of genome stability...
  15. ncbi Involvement of p54(nrb), a PSF partner protein, in DNA double-strand break repair and radioresistance
    Shuyi Li
    Department of Medicine, Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, Georgia, USA
    Nucleic Acids Res 37:6746-53. 2009
    ..Together, results indicate that p54(nrb) contributes to rapid and accurate repair of DSBs in vivo in human cells and that the PSF.p54(nrb) complex may thus be a potential target for radiosensitizer development...
  16. ncbi Distinct pathways of nonhomologous end joining that are differentially regulated by DNA-dependent protein kinase-mediated phosphorylation
    Durga Udayakumar
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, GA 30912, USA
    J Biol Chem 278:41631-5. 2003
    ..The existence of two distinct pathways, which are differentially regulated by the DNA-dependent protein kinase, provides a possible explanation for the selective repair defects seen in DNA-dependent protein kinase-deficient mutants...
  17. ncbi Saturation labeling with cysteine-reactive cyanine fluorescent dyes provides increased sensitivity for protein expression profiling of laser-microdissected clinical specimens
    Olga Greengauz-Roberts
    Institute of Molecular Medicine and Genetics, Medical College of Georgia, Augusta, 30904, USA
    Proteomics 5:1746-57. 2005
    ..The identification of a number of proteins that are potentially relevant to tumor progression suggests that the method holds promise for biomarker discovery...
  18. ncbi Human papillomavirus-active head and neck cancer and ethnic health disparities
    Paul M Weinberger
    Department of Otolaryngology, Medical College of Georgia, Augusta, Georgia, USA
    Laryngoscope 120:1531-7. 2010
    ..We hypothesized that prevalence of this HPV-active disease class would be lower in black HNSCC patients compared to white patients...

Research Grants14

  1. Laser Capture Microscopy and 2D-DIGE: Cancer Proteomics
    William Dynan; Fiscal Year: 2007
    ..In the next phase, broader clinical utility will be demonstrated using a larger sample set. Changes in the pattern of protein expression will be identified. These changes can serve as clinically useful markers of tumor progression. ..
  2. Regulation of DNA Double-Strand Break Repair
    William Dynan; Fiscal Year: 2007
    ..An overall goal is to obtain basic insights into the regulation of DSB repair that will lead to new, mechanism-based strategies for increasing the efficacy of radiation therapy. ..
  3. INTERACTIONS OF DNA-PK WITH THE TRANSCRIPTION APPARATUS
    William Dynan; Fiscal Year: 2004
    ..Experiments in this proposal will provide definitive information about signaling interactions that occur between DNA-PK and the transcription apparatus. ..
  4. Investigation of a Novel Role for RNA Binding Proteins in DNA Repair
    William S Dynan; Fiscal Year: 2010
    ..The project has potential clinical and translational impact because of the possibility that therapeutic RNAs might be developed to inhibit the DNA repair activity of these proteins and thus alter the clinical radiation response. ..