WILLIAM BRYSON CAMPBELL

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. pmc Endothelial metabolism of angiotensin II to angiotensin III, not angiotensin (1-7), augments the vasorelaxation response in adrenal cortical arteries
    Phillip G Kopf
    PhD, Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226
    Endocrinology 154:4768-76. 2013
  2. pmc Inducible endothelium-derived hyperpolarizing factor: role of the 15-lipoxygenase-EDHF pathway
    William B Campbell
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Cardiovasc Pharmacol 61:176-87. 2013
  3. ncbi request reprint What is new in endothelium-derived hyperpolarizing factors?
    William B Campbell
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Curr Opin Nephrol Hypertens 11:177-83. 2002
  4. ncbi request reprint Arachidonic acid metabolites as endothelium-derived hyperpolarizing factors
    William B Campbell
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Hypertension 49:590-6. 2007
  5. ncbi request reprint Regulation of potassium channels in coronary smooth muscle by adenoviral expression of cytochrome P-450 epoxygenase
    William B Campbell
    Dept of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 290:H64-71. 2006
  6. ncbi request reprint 11,12,15-Trihydroxyeicosatrienoic acid mediates ACh-induced relaxations in rabbit aorta
    William B Campbell
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 285:H2648-56. 2003
  7. pmc Epoxyeicosatrienoic acids and endothelium-dependent responses
    William B Campbell
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Pflugers Arch 459:881-95. 2010
  8. ncbi request reprint 14,15-Dihydroxyeicosatrienoic acid relaxes bovine coronary arteries by activation of K(Ca) channels
    William B Campbell
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Am J Physiol Heart Circ Physiol 282:H1656-64. 2002
  9. pmc Angiotensin II regulates adrenal vascular tone through zona glomerulosa cell-derived EETs and DHETs
    Phillip G Kopf
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Hypertension 57:323-9. 2011
  10. pmc 14,15-Dihydroxy-eicosa-5(Z)-enoic acid selectively inhibits 14,15-epoxyeicosatrienoic acid-induced relaxations in bovine coronary arteries
    Ishfaq A Bukhari
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 336:47-55. 2011

Research Grants

  1. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    WILLIAM BRYSON CAMPBELL; Fiscal Year: 2010
  2. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 2006
  3. EICOSANOID SYNTHESIS AND FUNCTION IN CORONARY VESSELS
    William Campbell; Fiscal Year: 2005
  4. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 2005
  5. Nitric Oxide Regulation of Adrenal Steroidogenesis
    William Campbell; Fiscal Year: 2004
  6. BIOCHEMISTRY OF ANANDAMIDE, AN ENDOGENOUS CANNABINOID
    William Campbell; Fiscal Year: 2004
  7. EICOSANOID SYNTHESIS AND FUNCTION IN CORONARY VESSELS
    William Campbell; Fiscal Year: 2004
  8. LC-MS/MS for Biomedical Research
    William Campbell; Fiscal Year: 2004
  9. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 2004
  10. Nitric Oxide Regulation of Adrenal Steroidogenesis
    William Campbell; Fiscal Year: 2003

Collaborators

Detail Information

Publications68

  1. pmc Endothelial metabolism of angiotensin II to angiotensin III, not angiotensin (1-7), augments the vasorelaxation response in adrenal cortical arteries
    Phillip G Kopf
    PhD, Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226
    Endocrinology 154:4768-76. 2013
    ..These data indicate that Ang II metabolism modulates the vascular effects of Ang II in the adrenal vasculature. ..
  2. pmc Inducible endothelium-derived hyperpolarizing factor: role of the 15-lipoxygenase-EDHF pathway
    William B Campbell
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Cardiovasc Pharmacol 61:176-87. 2013
    ..This new EDHF classification provides a framework for understanding EDHF activity in physiological and pathological conditions...
  3. ncbi request reprint What is new in endothelium-derived hyperpolarizing factors?
    William B Campbell
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Curr Opin Nephrol Hypertens 11:177-83. 2002
    ..This article reviews the latest studies concerning the characterization of endothelium-derived hyperpolarizing factors, the mechanisms of factor release and alterations of the factors...
  4. ncbi request reprint Arachidonic acid metabolites as endothelium-derived hyperpolarizing factors
    William B Campbell
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Hypertension 49:590-6. 2007
    ..These findings indicate that epoxyeicosatrienoic acids act as endothelium-derived hyperpolarizing factors and regulate arterial tone...
  5. ncbi request reprint Regulation of potassium channels in coronary smooth muscle by adenoviral expression of cytochrome P-450 epoxygenase
    William B Campbell
    Dept of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 290:H64-71. 2006
    ..These studies indicate that 14(S),15(R)-EET is a sufficient endogenous activator of BK(Ca) channels in coronary SMCs...
  6. ncbi request reprint 11,12,15-Trihydroxyeicosatrienoic acid mediates ACh-induced relaxations in rabbit aorta
    William B Campbell
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 285:H2648-56. 2003
    ..Additionally, this lipoxygenase pathway mediates the nonnitric oxide, nonprostaglandin relaxations to ACh in the rabbit aorta by acting as a source of an endothelium-derived hyperpolarizing factor...
  7. pmc Epoxyeicosatrienoic acids and endothelium-dependent responses
    William B Campbell
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Pflugers Arch 459:881-95. 2010
    ..Thus, the EETs are endothelium-derived hyperpolarizing factors mediating a portion of the relaxations to acetylcholine, bradykinin, shear stress, and cyclic stretch and regulate vascular tone in vitro and in vivo...
  8. ncbi request reprint 14,15-Dihydroxyeicosatrienoic acid relaxes bovine coronary arteries by activation of K(Ca) channels
    William B Campbell
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Am J Physiol Heart Circ Physiol 282:H1656-64. 2002
    ..14,15-DHET relaxes coronary arteries through activation of K(Ca) channels. Endothelial cells, but not smooth muscle cells, convert EETs to DHETs, and this conversion results in a loss of vasodilator activity...
  9. pmc Angiotensin II regulates adrenal vascular tone through zona glomerulosa cell-derived EETs and DHETs
    Phillip G Kopf
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Hypertension 57:323-9. 2011
    ..This provides a mechanism by which Ang II concurrently increases adrenal blood flow and steroidogenesis...
  10. pmc 14,15-Dihydroxy-eicosa-5(Z)-enoic acid selectively inhibits 14,15-epoxyeicosatrienoic acid-induced relaxations in bovine coronary arteries
    Ishfaq A Bukhari
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 336:47-55. 2011
    ..The present study indicates that conversion of 14,15-EE5ZE to 14,15-DHE5ZE produces a 14,15-EET-selective antagonist that will be a useful pharmacological tool to identify EET receptor(s) and EET function in the cardiovascular system...
  11. pmc Adrenic acid metabolites as endogenous endothelium-derived and zona glomerulosa-derived hyperpolarizing factors
    Phillip G Kopf
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wis 53226, USA
    Hypertension 55:547-54. 2010
    ..Thus, adrenic acid metabolites could function as endogenous endothelium-derived and zona glomerulosa-derived hyperpolarizing factors in the adrenal cortex and contribute to the regulation of adrenal blood flow...
  12. pmc Epoxygenase eicosanoids: synthesis of tetrahydrofuran-diol metabolites and their vasoactivity
    J R Falck
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA
    Bioorg Med Chem Lett 17:2634-8. 2007
    ..The THFDs potently induced relaxation of pre-contracted bovine arteries...
  13. pmc Studies of anandamide accumulation inhibitors in cerebellar granule neurons: comparison to inhibition of fatty acid amide hydrolase
    Cecilia J Hillard
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Mol Neurosci 33:18-24. 2007
    ....
  14. ncbi request reprint Endothelium-derived steroidogenic factor enhances angiotensin II-stimulated aldosterone release by bovine zona glomerulosa cells
    Craig J Hanke
    Department of Human Biology, University of Wisconsin Green Bay, Wisconsin 54301, USA
    Endocrinology 148:317-23. 2007
    ..These studies indicate that EDSF enhances the steroidogenic effect of AII. In the adrenal gland, ECs are in close anatomical relationship with ZG cells and may sensitize ZG cells to the steroidogenic action of AII by releasing EDSF...
  15. ncbi request reprint Adenoviral expression of 15-lipoxygenase-1 in rabbit aortic endothelium: role in arachidonic acid-induced relaxation
    Nitin T Aggarwal
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 292:H1033-41. 2007
    ..This confirms the role of HEETA and THETA as endothelium-derived relaxing factors...
  16. ncbi request reprint Reduced expression of 15-lipoxygenase 2 in human head and neck carcinomas
    Dian Wang
    Department of Radiation Oncology, Medical College of Wisconsin, Milwaukee, Wisc, USA
    Tumour Biol 27:261-73. 2006
    ..Our results demonstrate that 15-LOX-2 expression is significantly reduced and this reduction may promote proliferation in human head and neck carcinoma. 15-LOX-2 may be a possible biomarker in human head and neck malignancy...
  17. ncbi request reprint Chiral resolution of the epoxyeicosatrienoic acids, arachidonic acid epoxygenase metabolites
    Shouzou Wei
    Department of Medicine, Vanderbilt Medical School, Nashville, TN 37232, USA
    Anal Biochem 352:129-34. 2006
    ..Application of this methodology to the analysis of the liver endogenous EETs demonstrates stereospecific biosynthesis and corroborates the role of cytochrome P450 as the endogenous arachidonic acid epoxygenase...
  18. ncbi request reprint A role for heterocellular coupling and EETs in dilation of rat cremaster arteries
    Iain N McSherry
    University of Bath, Bath, United Kingdom
    Microcirculation 13:119-30. 2006
    ..The authors probed endothelium-dependent dilation and endothelial cell Ca2+ handling in myogenically active resistance arteries...
  19. pmc Epoxyeicosatrienoic and dihydroxyeicosatrienoic acids dilate human coronary arterioles via BK(Ca) channels: implications for soluble epoxide hydrolase inhibition
    Brandon T Larsen
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 290:H491-9. 2006
    ..CYP450s and sEH may be endogenous sources of these compounds, and sEH inhibition has the potential to alter myocardial perfusion, depending on which EETs are produced endogenously...
  20. ncbi request reprint Reticulocyte 15-lipoxygenase-I is important in acetylcholine-induced endothelium-dependent vasorelaxation in rabbit aorta
    Xin Tang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Arterioscler Thromb Vasc Biol 26:78-84. 2006
    ..These metabolites contribute to endothelium-dependent relaxations of rabbit aorta to AA and acetylcholine. We investigated the identity of rabbit aortic 15-LO and studied its importance in the regulation of vascular tone...
  21. ncbi request reprint Effects of selective inhibition of cytochrome P-450 omega-hydroxylases and ischemic preconditioning in myocardial protection
    Kasem Nithipatikom
    Dept of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 290:H500-5. 2006
    ....
  22. ncbi request reprint Characterization of 14,15-epoxyeicosatrienoyl-sulfonamides as 14,15-epoxyeicosatrienoic acid agonists: use for studies of metabolism and ligand binding
    Wenqi Yang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 321:1023-31. 2007
    ..14,15-EET-P(125)ISA is a new radiolabeled tool to study EET metabolism and binding. Our results also provide preliminary evidence that EETs exert their biological effect through a membrane binding site/receptor...
  23. ncbi request reprint ACh-induced relaxations of rabbit small mesenteric arteries: role of arachidonic acid metabolites and K+
    David X Zhang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 293:H152-9. 2007
    ....
  24. pmc Diverse roles of 2-arachidonoylglycerol in invasion of prostate carcinoma cells: Location, hydrolysis and 12-lipoxygenase metabolism
    Michael P Endsley
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI, USA
    Int J Cancer 121:984-91. 2007
    ..Therefore, inhibitors of 2-AG hydrolysis could potentially serve as therapeutic agents for the treatment of prostate cancer. (c) 2007 Wiley-Liss, Inc...
  25. pmc Expression and function of fatty acid amide hydrolase in prostate cancer
    Michael P Endsley
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Int J Cancer 123:1318-26. 2008
    ..These results suggest that FAAH regulates 2-AG hydrolysis and invasion of prostate carcinoma cells and is potentially involved in prostate tumorigenesis...
  26. pmc Angiotensin II relaxations of bovine adrenal cortical arteries: role of angiotensin II metabolites and endothelial nitric oxide
    Kathryn M Gauthier
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Hypertension 52:150-5. 2008
    ....
  27. pmc 15-Lipoxygenase metabolites contribute to age-related reduction in acetylcholine-induced hypotension in rabbits
    Nitin T Aggarwal
    Dept of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 295:H89-96. 2008
    ..5 +/- 1.4%, and by BW755C to -18.8 +/- 3.5%. The present study indicates that the age-related decrease in ACh-induced hypotension is mediated by the decreased synthesis of the 15-LO-1 metabolites THETAs and HEETAs...
  28. pmc Effects of the selective EET antagonist, 14,15-EEZE, on cardioprotection produced by exogenous or endogenous EETs in the canine heart
    Garrett J Gross
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 294:H2838-44. 2008
    ..These data also suggest that 14,15-EEZE is not blocking the mitochondrial ATP-sensitive potassium channel as a mechanism for antagonizing the cardioprotective effects of the EETs...
  29. doi request reprint 11(R),12(S),15(S)-trihydroxyeicosa-5(Z),8(Z),13(E)-trienoic acid: an endothelium-derived 15-lipoxygenase metabolite that relaxes rabbit aorta
    Kathryn M Gauthier
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 294:H1467-72. 2008
    ..It relaxes smooth muscle by activating K+ channels. The specific structural and stereochemical requirements for K+ channel activation suggest that a specific binding site or receptor of 11,12,15-THETA is involved in these actions...
  30. doi request reprint Identification of 15-hydroxy-11,12-epoxyeicosatrienoic acid as a vasoactive 15-lipoxygenase metabolite in rabbit aorta
    Yuttana Chawengsub
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 294:H1348-56. 2008
    ..These results indicate that 15-H-11,12-EETA is produced by the aorta, hydrolyzed by sEH to 11,12,15-THETA, and mediates relaxations by membrane hyperpolarization. 15-H-11,12-EETA represents an endothelium-derived hyperpolarizing factor...
  31. doi request reprint Endothelial 15-lipoxygenase-1 overexpression increases acetylcholine-induced hypotension and vasorelaxation in rabbits
    Nitin T Aggarwal
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Hypertension 51:246-51. 2008
    ..Therefore, increased expression of 15-LO-1 increases acetylcholine relaxation in arteries and hypotensive responses in rabbits because of increased hydroxy-epoxyeicosatrienoic acid and trihydroxyeicosatrienoic acid synthesis...
  32. ncbi request reprint Roles of epoxyeicosatrienoic acids in vascular regulation and cardiac preconditioning
    Kathryn M Gauthier
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Cardiovasc Pharmacol 50:601-8. 2007
    ..Production and metabolism of EETs as well as their specific cellular signaling mechanisms are discussed...
  33. pmc Hydrogen peroxide inhibits cytochrome p450 epoxygenases: interaction between two endothelium-derived hyperpolarizing factors
    Brandon T Larsen
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Circ Res 102:59-67. 2008
    ..These findings suggest that an inhibitory interaction exists between 2 EDHFs in the human coronary microcirculation. CYP epoxygenases are directly inhibited by H2O2, and this interaction may modulate vascular EET bioavailability...
  34. ncbi request reprint Steroid-producing cells regulate arterial tone of adrenal cortical arteries
    David X Zhang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
    Endocrinology 148:3569-76. 2007
    ..We conclude that ZG cells release EETs and this release is stimulated by ACTH. Interaction of endocrine and vascular cells represents a mechanism for regulating adrenal blood flow and couples steroidogenesis to increased blood flow...
  35. ncbi request reprint Age-related decrease in 15-lipoxygenase contributes to reduced vasorelaxation in rabbit aorta
    Xin Tang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 294:H679-87. 2008
    ..These data indicate that aortic 15-LO-1 expression and activity are downregulated with aging in rabbits. This decrease is paralleled by the reduced synthesis of vasoactive THETA and HEETA and aortic relaxations to acetylcholine and AA...
  36. ncbi request reprint Novel mechanism of vasodilation in inflammatory bowel disease
    Ossama A Hatoum
    Department of Medicine, Zablocki VA Medical Center, Medical College of Wisconsin, Milwaukee, WI, USA
    Arterioscler Thromb Vasc Biol 25:2355-61. 2005
    ..The contributions of both nitric oxide (NO) and endothelial-derived hyperpolarizing factor (EDHF) are decreased. We hypothesized that the remaining dilation results from products of cyclooxygenase...
  37. ncbi request reprint Mechanisms of histamine-induced relaxation in bovine small adrenal cortical arteries
    David X Zhang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Am J Physiol Endocrinol Metab 289:E1058-63. 2005
    ..The potential role of histamine in linking adrenal vascular events and steroid secretion requires further investigation...
  38. ncbi request reprint Apamin-sensitive K+ currents mediate arachidonic acid-induced relaxations of rabbit aorta
    Kathryn M Gauthier
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Hypertension 43:413-9. 2004
    ..These results suggest that arachidonic acid induces endothelium-dependent hyperpolarization and relaxation of rabbit aorta through activation of smooth muscle, apamin-sensitive K+ currents...
  39. ncbi request reprint Rabbit aorta converts 15-HPETE to trihydroxyeicosatrienoic acids: potential role of cytochrome P450
    Sandra L Pfister
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Arch Biochem Biophys 420:142-52. 2003
    ..Further hydrolysis of the epoxy group of the HEETAs results in the formation of 11,12,15- and 11,14,15-THETAs. The HEETAs and THETAs are both vasodilators and may function as important regulators of vascular tone...
  40. ncbi request reprint Characterization and application of Raman labels for confocal Raman microspectroscopic detection of cellular proteins in single cells
    Kasem Nithipatikom
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Anal Biochem 322:198-207. 2003
    ..8 nm. The detection of 12-lipoxygenase and cyclooxygenase-1 in single bovine coronary artery endothelial cells and the binding of angiotensin II to its receptors in zona glomerulosa cells was demonstrated...
  41. ncbi request reprint 14,15-epoxyeicosa-5(Z)-enoic-mSI: a 14,15- and 5,6-EET antagonist in bovine coronary arteries
    Kathryn M Gauthier
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Hypertension 42:555-61. 2003
    ..It also inhibits the EDHF component of bradykinin-induced, methacholine-induced, and arachidonic acid-induced relaxation. These results suggest that 14,15- or 5,6 -EET act as an EDHF in bovine coronary arteries...
  42. ncbi request reprint Interleukin-13 upregulates vasodilatory 15-lipoxygenase eicosanoids in rabbit aorta
    Xin Tang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Arterioscler Thromb Vasc Biol 23:1768-74. 2003
    ..We tested the hypothesis that IL-13 upregulates the 15-lipoxygenase pathway in rabbit aorta by inducing 15-lipoxygenase expression, thus increasing vascular relaxation mediated by THETA and HEETA...
  43. ncbi request reprint Metabolism of 12-hydroperoxyeicosatetraenoic acid to vasodilatory trioxilin C3 by rabbit aorta
    Sandra L Pfister
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochim Biophys Acta 1622:6-13. 2003
    ..6+/-1.3%). In conclusion, trioxilin C(3) acid was isolated and identified as a novel biologically active arachidonic acid metabolite formed by rabbit aorta when 12-lipoxygenase is supplied exogenously...
  44. ncbi request reprint Chronic hypoxia activates lung 15-lipoxygenase, which catalyzes production of 15-HETE and enhances constriction in neonatal rabbit pulmonary arteries
    Daling Zhu
    Department of Medicine, Medical College of Wisconsin, Milwaukee, Wis 53226, USA
    Circ Res 92:992-1000. 2003
    ..Although a clear causal relationship remains to be demonstrated, these data suggest a previously unrecognized role for 15-LO in hypoxic vasoconstriction in neonatal mammals...
  45. ncbi request reprint Determination of cyclooxygenase and arachidonic acid metabolites in invasive human prostate cancer cells
    Kasem Nithipatikom
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Adv Exp Med Biol 507:269-74. 2002
  46. ncbi request reprint Cyclic ADP-ribose contributes to contraction and Ca2+ release by M1 muscarinic receptor activation in coronary arterial smooth muscle
    Zhi Dong Ge
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee 53226, USA
    J Vasc Res 40:28-36. 2003
    ..These results suggest that the cADPR signaling pathway participates in the contraction of small coronary arterial smooth muscle and Ca(2+) release induced by activation of M(1) muscarinic receptors...
  47. ncbi request reprint Endothelium-derived hyperpolarizing factor in human internal mammary artery is 11,12-epoxyeicosatrienoic acid and causes relaxation by activating smooth muscle BK(Ca) channels
    Stephen L Archer
    Vascular Biology Group, Cardiology Surgery, University of Alberta, Edmonton, Canada
    Circulation 107:769-76. 2003
    ..We hypothesized that EDHF (1) is 11,12-epoxyeicosatrienoic acid (11,12-EET); (2) is generated by cytochrome P450-2C, CYP450-2C; and (3) causes relaxation by opening SMC large-conductance Ca(2+)-activated K(+) channels (BK(Ca))...
  48. ncbi request reprint Liquid chromatographic-mass spectrometric determination of cyclooxygenase metabolites of arachidonic acid in cultured cells
    Kasem Nithipatikom
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee 53226, USA
    J Chromatogr B Analyt Technol Biomed Life Sci 785:135-45. 2003
    ..6-Keto-PGF(1alpha) was the major metabolite in these cells. The production of PGE(2) was threefold higher and PGD(2) was twofold higher in PC-3-S (invasive) cells than in PC-3-U (non-invasive) cells...
  49. ncbi request reprint Role of contaminating platelets in thromboxane synthesis in primary cultures of human umbilical vein endothelial cells
    Sandra L Pfister
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee 53226, USA
    Prostaglandins Other Lipid Mediat 70:39-49. 2002
    ..These studies suggest that primary cultures of human umbilical vein endothelial cells contain adherent platelets that contribute to thromboxane synthesis...
  50. ncbi request reprint Simultaneous in situ monitoring of intracellular Ca2+ and NO in endothelium of coronary arteries
    Fu Xian Yi
    Department of Pharmacology, Medical College of Wisconsin, Milwaukee 53226, USA
    Am J Physiol Heart Circ Physiol 283:H2725-32. 2002
    ....
  51. ncbi request reprint 14,15-Epoxyeicosa-5(Z)-enoic acid: a selective epoxyeicosatrienoic acid antagonist that inhibits endothelium-dependent hyperpolarization and relaxation in coronary arteries
    Kathryn M Gauthier
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee 53226, USA
    Circ Res 90:1028-36. 2002
    ..Thus, a distinct molecular structure is required for EET activity, and alteration of this structure modifies agonist and antagonist activity. These findings support a role of EETs as EDHFs...
  52. ncbi request reprint Membrane-potential-dependent inhibition of platelet adhesion to endothelial cells by epoxyeicosatrienoic acids
    Florian Krotz
    Institute of Physiology, Medizinische Poliklinik Innenstadt, Ludwig Maximilians University, Munchen, Germany
    Arterioscler Thromb Vasc Biol 24:595-600. 2004
    ..In many vessels, they are an endothelium-derived hyperpolarizing factor (EDHF). However, it is unknown whether they act as an EDHF on platelets and whether this has functional consequences...
  53. ncbi request reprint Characterization of vasoconstrictor responses in small bovine adrenal cortical arteries in vitro
    David X Zhang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee 53226, USA
    Endocrinology 145:1571-8. 2004
    ..The forceful contractile responses of these arterioles are consistent with their potential role in the regulation of adrenal blood flow...
  54. ncbi request reprint Role of phospholipase C and diacylglyceride lipase pathway in arachidonic acid release and acetylcholine-induced vascular relaxation in rabbit aorta
    Xin Tang
    Dept of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 290:H37-45. 2006
    ..The data suggested that ACh activates PLC, DAG lipase, and MAG lipase pathway to release AA from membrane lipids. This pathway is important in regulating vasodilatory eicosanoid synthesis and vascular relaxation in rabbit aorta...
  55. pmc A new class of inhibitors of 2-arachidonoylglycerol hydrolysis and invasion of prostate cancer cells
    Kasem Nithipatikom
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Biochem Biophys Res Commun 332:1028-33. 2005
    ..These results demonstrate a new series of 2-AG hydrolysis inhibitors as a potential therapeutic approach for prostate cancer...
  56. pmc Bradykinin-induced, endothelium-dependent responses in porcine coronary arteries: involvement of potassium channel activation and epoxyeicosatrienoic acids
    Arthur H Weston
    Faculty of Life Sciences, University of Manchester, G38 Stopford Building, Manchester M13 9PT
    Br J Pharmacol 145:775-84. 2005
    ..These open endothelial IK(Ca) and SK(Ca) channels and also activate large-conductance calcium-sensitive K+ channels (BK(Ca)) on the surrounding myocytes...
  57. ncbi request reprint Angiotensin II dilates bovine adrenal cortical arterioles: role of endothelial nitric oxide
    Kathryn M Gauthier
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Endocrinology 146:3319-24. 2005
    ..Our results demonstrate that AII stimulates adrenal cortical arterial dilation through endothelial cell AT2 receptor activation and NO release and AT1 receptor-dependent constriction...
  58. ncbi request reprint Elevated 12- and 20-hydroxyeicosatetraenoic acid in urine of patients with prostatic diseases
    Kasem Nithipatikom
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Cancer Lett 233:219-25. 2006
    ..These results suggest that urinary free acids of 12-HETE and 20-HETE indicate an abnormality of the prostate gland...
  59. ncbi request reprint 14,15-epoxyeicosatrienoic acid represents a transferable endothelium-dependent relaxing factor in bovine coronary arteries
    Kathryn M Gauthier
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Hypertension 45:666-71. 2005
    ..14,15-EEZE blocked the donor artery, endothelium-dependent, bradykinin-induced relaxations, and attenuated relaxations to 14,15-EET. These results suggest that EETs are transferable EDHFs in coronary arteries...
  60. ncbi request reprint Stable 5,6-epoxyeicosatrienoic acid analog relaxes coronary arteries through potassium channel activation
    Wenqi Yang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Hypertension 45:681-6. 2005
    ..PDPAs only activate large conductance calcium-activated K+ channels...
  61. ncbi request reprint Measurement of steroid synthesis in zona glomerulosa cells by liquid chromatography-electrospray ionization-mass spectrometry: inhibition by nitric oxide
    Kasem Nithipatikom
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Anal Biochem 337:203-10. 2005
    ..The technique demonstrates the ability to determine the individual steroid in each enzymatic step of aldosterone synthesis and the activity of steroidogenic enzymes in adrenal ZG cells...
  62. ncbi request reprint Endothelium-derived 2-arachidonylglycerol: an intermediate in vasodilatory eicosanoid release in bovine coronary arteries
    Kathryn M Gauthier
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Am J Physiol Heart Circ Physiol 288:H1344-51. 2005
    ..2-AG is further hydrolyzed to AA, which is metabolized to vasoactive eicosanoids. These studies reveal a role for 2-AG in EC AA release and the regulation of coronary tone...
  63. ncbi request reprint Endostatin uncouples NO and Ca2+ response to bradykinin through enhanced O2*- production in the intact coronary endothelium
    Andrew Y Zhang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Am J Physiol Heart Circ Physiol 288:H686-94. 2005
    ..production through activation of NAD(P)H oxidase. This ceramide-O2*-. signaling pathway may contribute importantly to endostatin-induced endothelial dysfunction...
  64. ncbi request reprint Inhibition of cytochrome P450omega-hydroxylase: a novel endogenous cardioprotective pathway
    Kasem Nithipatikom
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Circ Res 95:e65-71. 2004
    ..The full text of this article is available online at http://circres.ahajournals.org...
  65. ncbi request reprint Asymmetric synthesis of the stereoisomers of 11,12,15(S)-trihydroxyeicosa-5(Z),8(Z),13(E)-trienoic acid, a potent endothelium-derived vasodilator
    J R Falck
    Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390 9038, USA
    Bioorg Med Chem Lett 14:4987-90. 2004
    ..Only 11(R),12(S),15(S)-THETA was comparable to natural material by HPLC, GC/MS, and in vitro bioassay...
  66. ncbi request reprint Acetylcholine-induced relaxation and hyperpolarization in small bovine adrenal cortical arteries: role of cytochrome P450 metabolites
    David X Zhang
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
    Endocrinology 145:4532-9. 2004
    ..The acetylcholine-induced relaxation is largely mediated by an endothelium-dependent hyperpolarization mechanism, presumably through cytochrome P450 metabolites of arachidonic acid...
  67. ncbi request reprint Glibenclamide inhibits thromboxane-mediated vasoconstriction by thromboxane receptor blockade
    Sandra L Pfister
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Vascul Pharmacol 40:285-92. 2004
    ..These studies provide the first evidence that the ability of glibenclamide to inhibit TX-mediated contractions occurs independent of the vascular K(ATP) channel and is, instead, mediated by the blockade of the vascular TX receptor...
  68. ncbi request reprint Role of ADP-ribose in 11,12-EET-induced activation of K(Ca) channels in coronary arterial smooth muscle cells
    Pin Lan Li
    Department of Pharmacology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Am J Physiol Heart Circ Physiol 282:H1229-36. 2002
    ....

Research Grants54

  1. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    WILLIAM BRYSON CAMPBELL; Fiscal Year: 2010
    ..These studies will test the hypothesis that endogenous LO metabolites are important regulators of vascular tone and blood pressure in mice. ..
  2. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 2006
    ....
  3. EICOSANOID SYNTHESIS AND FUNCTION IN CORONARY VESSELS
    William Campbell; Fiscal Year: 2005
    ..We will determine if conferring cytochrome P450 epoxygenase activity on smooth muscle cells allows endogenously formed EETs to activate Kca channel activity and cause endothelium-independent relaxation. ..
  4. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 2005
    ....
  5. Nitric Oxide Regulation of Adrenal Steroidogenesis
    William Campbell; Fiscal Year: 2004
    ..4) We will determine the role of endothelial NO in regulating steroidogenesis and adrenal blood flow in the in situ perfused adrenal gland and in vivo in anesthetized rats. ..
  6. BIOCHEMISTRY OF ANANDAMIDE, AN ENDOGENOUS CANNABINOID
    William Campbell; Fiscal Year: 2004
    ..We anticipate that successful completion of these studies will provide valuable insight into the role of the AEA/CB 1 signaling system in the brain. ..
  7. EICOSANOID SYNTHESIS AND FUNCTION IN CORONARY VESSELS
    William Campbell; Fiscal Year: 2004
    ..We will determine if conferring cytochrome P450 epoxygenase activity on smooth muscle cells allows endogenously formed EETs to activate Kca channel activity and cause endothelium-independent relaxation. ..
  8. LC-MS/MS for Biomedical Research
    William Campbell; Fiscal Year: 2004
    ..About 75-80% of instrument time will be available to these participants and the remaining time will be available to other investigators at the College. ..
  9. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 2004
    ..4. investigate the mechanism of smooth muscle dilation by 15-H-11,12-EETA and 11,12,15-THETA by determining their action on membrane potential and potassium channel activity. ..
  10. Nitric Oxide Regulation of Adrenal Steroidogenesis
    William Campbell; Fiscal Year: 2003
    ..4) We will determine the role of endothelial NO in regulating steroidogenesis and adrenal blood flow in the in situ perfused adrenal gland and in vivo in anesthetized rats. ..
  11. BIOCHEMISTRY OF ANANDAMIDE, AN ENDOGENOUS CANNABINOID
    William Campbell; Fiscal Year: 2003
    ..We anticipate that successful completion of these studies will provide valuable insight into the role of the AEA/CB 1 signaling system in the brain. ..
  12. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 2003
    ..4. investigate the mechanism of smooth muscle dilation by 15-H-11,12-EETA and 11,12,15-THETA by determining their action on membrane potential and potassium channel activity. ..
  13. Regulation Adrenal Vascular Tone by Steroidogenic Cells
    William Campbell; Fiscal Year: 2006
    ..The regulation of adrenal vascular tone by ZG cells may have broader implications. This may represent a general pathway for regulation of vascular tone in many endocrine glands. ..
  14. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 2007
    ....
  15. EICOSANOID SYNTHESIS AND FUNCTION IN CORONARY VESSELS
    William Campbell; Fiscal Year: 2007
    ..These studies will provide specific pharmacological tools to study the role of EETs as EDHFs and provide new insights into the vascular action of the EETs. ..
  16. Regulation of Adrenal Vascular Tone by Steroidogenic Cells
    WILLIAM BRYSON CAMPBELL; Fiscal Year: 2010
    ..The regulation of adrenal vascular tone by ZG cells may have broader implications. This may represent a general pathway for regulation of vascular tone in many endocrine glands. ..
  17. EICOSANOID SYNTHESIS AND FUNCTION IN CORONARY VESSELS
    WILLIAM BRYSON CAMPBELL; Fiscal Year: 2010
    ..These studies will provide specific pharmacological tools to study the role of EETs as EDHFs and provide new insights into the vascular action of the EETs. ..
  18. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 2009
    ..These studies will test the hypothesis that endogenous LO metabolites are important regulators of vascular tone and blood pressure in mice. ..
  19. Regulation of Adrenal Vascular Tone by Steroidogenic Cells
    William Campbell; Fiscal Year: 2009
    ..The regulation of adrenal vascular tone by ZG cells may have broader implications. This may represent a general pathway for regulation of vascular tone in many endocrine glands. ..
  20. EICOSANOID SYNTHESIS AND FUNCTION IN CORONARY VESSELS
    William Campbell; Fiscal Year: 2009
    ..These studies will provide specific pharmacological tools to study the role of EETs as EDHFs and provide new insights into the vascular action of the EETs. ..
  21. BIOCHEMISTRY OF ANANDAMIDE, AN ENDOGENOUS CANNABINOID
    William Campbell; Fiscal Year: 2007
    ..We have coupled this technique with our ability to culture cells from the cerebellum as a unique approach to these goals. ..
  22. Regulation of Adrenal Vascular Tone by Steroidogenic Cells
    William Campbell; Fiscal Year: 2007
    ..The regulation of adrenal vascular tone by ZG cells may have broader implications. This may represent a general pathway for regulation of vascular tone in many endocrine glands. ..
  23. EICOSANOID SYNTHESIS AND FUNCTION IN CORONARY VESSELS
    William Campbell; Fiscal Year: 2003
    ..We will determine if conferring cytochrome P450 epoxygenase activity on smooth muscle cells allows endogenously formed EETs to activate Kca channel activity and cause endothelium-independent relaxation. ..
  24. Nitric Oxide Regulation of Adrenal Steroidogenesis
    William Campbell; Fiscal Year: 2002
    ..4) We will determine the role of endothelial NO in regulating steroidogenesis and adrenal blood flow in the in situ perfused adrenal gland and in vivo in anesthetized rats. ..
  25. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 1999
    ....
  26. BIOCHEMISTRY OF ANANDAMIDE, AN ENDOGENOUS CANNABINOID
    William Campbell; Fiscal Year: 1999
    ..In addition, we will develop several tools that will be valuable additions to the cannabinoid field in general, including a sensitive assay for AEA and a series of active and inactive analogs. ..
  27. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 1993
  28. BIOCHEMISTRY OF ANANDAMIDE, AN ENDOGENOUS CANNABINOID
    William Campbell; Fiscal Year: 2002
    ..We anticipate that successful completion of these studies will provide valuable insight into the role of the AEA/CB 1 signaling system in the brain. ..
  29. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 2002
    ..4. investigate the mechanism of smooth muscle dilation by 15-H-11,12-EETA and 11,12,15-THETA by determining their action on membrane potential and potassium channel activity. ..
  30. EICOSANOID SYNTHESIS AND FUNCTION IN CORONARY VESSELS
    William Campbell; Fiscal Year: 2002
    ..We will determine if conferring cytochrome P450 epoxygenase activity on smooth muscle cells allows endogenously formed EETs to activate Kca channel activity and cause endothelium-independent relaxation. ..
  31. EICOSANOID SYNTHESIS AND FUNCTION IN CORONARY VESSELS
    William Campbell; Fiscal Year: 2001
    ..Measurement of the metabolites, inhibitors of eicosanoid synthesis and K channels and infusions of the eicosanoids should provide insights into the contribution of the EETs to coronary microvascular tone. ..
  32. BIOCHEMISTRY OF ANANDAMIDE, AN ENDOGENOUS CANNABINOID
    William Campbell; Fiscal Year: 2001
    ..We anticipate that successful completion of these studies will provide valuable insight into the role of the AEA/CB 1 signaling system in the brain. ..
  33. Nitric Oxide Regulation of Adrenal Steroidogenesis
    William Campbell; Fiscal Year: 2001
    ..4) We will determine the role of endothelial NO in regulating steroidogenesis and adrenal blood flow in the in situ perfused adrenal gland and in vivo in anesthetized rats. ..
  34. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 2001
    ..4. investigate the mechanism of smooth muscle dilation by 15-H-11,12-EETA and 11,12,15-THETA by determining their action on membrane potential and potassium channel activity. ..
  35. BIOCHEMISTRY OF ANANDAMIDE, AN ENDOGENOUS CANNABINOID
    William Campbell; Fiscal Year: 2000
    ..We anticipate that successful completion of these studies will provide valuable insight into the role of the AEA/CB 1 signaling system in the brain. ..
  36. EICOSANOID SYNTHESIS AND FUNCTION IN CORONARY VESSELS
    William Campbell; Fiscal Year: 2000
    ..Measurement of the metabolites, inhibitors of eicosanoid synthesis and K channels and infusions of the eicosanoids should provide insights into the contribution of the EETs to coronary microvascular tone. ..
  37. EICOSANOIDS, VASCULAR TONE AND BLOOD PRESSURE
    William Campbell; Fiscal Year: 2000
    ....
  38. EICOSANOID SYNTHESIS AND FUNCTION IN CORONARY VESSELS
    William Campbell; Fiscal Year: 1999
    ..Measurement of the metabolites, inhibitors of eicosanoid synthesis and K channels and infusions of the eicosanoids should provide insights into the contribution of the EETs to coronary microvascular tone. ..