Zeljko Bosnjak

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. pmc Developmental neurotoxicity screening using human embryonic stem cells
    Zeljko J Bosnjak
    Department of Anesthesiology, Medical College of Wisconsin, 8701 W Watertown Plank Rd, Milwaukee, WI 53226, USA
    Exp Neurol 237:207-10. 2012
  2. pmc Personal narrative of prof. Zeljko Bosnjak, Laureate of Croatian National Decoration for Humanitarian Work
    Zeljko Bosnjak
    Croat Med J 47:805-8. 2006
  3. pmc Generation of human induced pluripotent stem cells by simple transient transfection of plasmid DNA encoding reprogramming factors
    Karim Si-Tayeb
    Department of Cell Biology, The Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    BMC Dev Biol 10:81. 2010
  4. ncbi request reprint Zeljko J. Bosnjak, Ph.D., recipient of the 2008 Excellence in Research Award
    David C Warltier
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Anesthesiology 109:371-3. 2008
  5. ncbi request reprint Isoflurane inhibits cardiac myocyte apoptosis during oxidative and inflammatory stress by activating Akt and enhancing Bcl-2 expression
    Marina Jamnicki-Abegg
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesthesiology 103:1006-14. 2005
  6. ncbi request reprint Characterization of human cardiac mitochondrial ATP-sensitive potassium channel and its regulation by phorbol ester in vitro
    Ming Tao Jiang
    Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 290:H1770-6. 2006
  7. ncbi request reprint Impact of in vivo preconditioning by isoflurane on adenosine triphosphate-sensitive potassium channels in the rat heart: lasting modulation of nucleotide sensitivity during early memory period
    Anna Stadnicka
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesthesiology 104:503-10. 2006
  8. ncbi request reprint Distinct roles for sarcolemmal and mitochondrial adenosine triphosphate-sensitive potassium channels in isoflurane-induced protection against oxidative stress
    Jasna Marinovic
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226, USA
    Anesthesiology 105:98-104. 2006
  9. ncbi request reprint Mechanism of cardiac sarcolemmal adenosine triphosphate-sensitive potassium channel activation by isoflurane in a heterologous expression system
    Martin Bienengraeber
    Department of Anesthesiology, Medical College of Wisconsin, Wisconsin 53226, USA
    Anesthesiology 105:534-40. 2006
  10. ncbi request reprint Anesthetic preconditioning enhances Ca2+ handling and mechanical and metabolic function elicited by Na+-Ca2+ exchange inhibition in isolated hearts
    Jianzhong An
    Department of Anesthesiology, Medical College of Wisconsin, Wisconsin 53226, USA
    Anesthesiology 105:541-9. 2006

Research Grants

  1. ANESTHETICS AND CARDIAC SIGNAL TRANSDUCTION
    Zeljko Bosnjak; Fiscal Year: 2003
  2. ANESTHETIC-INDUCED CARDIAC PRECONDITIONING
    Zeljko Bosnjak; Fiscal Year: 2007
  3. ANESTHETICS AND CARDIAC SIGNAL TRANSDUCTION
    Zeljko Bosnjak; Fiscal Year: 2009
  4. INTERACTION BETWEEN ANESTHETICS AND CALCIUM ANTAGONISTS
    Zeljko Bosnjak; Fiscal Year: 1993
  5. ANESTHETICS AND CARDIAC SIGNAL TRANSDUCTION
    Zeljko Bosnjak; Fiscal Year: 1999
  6. ANESTHETICS AND CARDIAC SIGNAL TRANSDUCTION
    Zeljko J Bosnjak; Fiscal Year: 2010

Collaborators

Detail Information

Publications40

  1. pmc Developmental neurotoxicity screening using human embryonic stem cells
    Zeljko J Bosnjak
    Department of Anesthesiology, Medical College of Wisconsin, 8701 W Watertown Plank Rd, Milwaukee, WI 53226, USA
    Exp Neurol 237:207-10. 2012
    ..The ultimate goal would be not only to find the trigger for the catastrophic chain of events, but also to prevent neuronal cell death itself...
  2. pmc Personal narrative of prof. Zeljko Bosnjak, Laureate of Croatian National Decoration for Humanitarian Work
    Zeljko Bosnjak
    Croat Med J 47:805-8. 2006
  3. pmc Generation of human induced pluripotent stem cells by simple transient transfection of plasmid DNA encoding reprogramming factors
    Karim Si-Tayeb
    Department of Cell Biology, The Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    BMC Dev Biol 10:81. 2010
    ..Commonly the procedures require either subcloning to identify human iPS cells that are free of exogenous DNA, a knowledge of virology and safe handling procedures, or a detailed understanding of protein biochemistry...
  4. ncbi request reprint Zeljko J. Bosnjak, Ph.D., recipient of the 2008 Excellence in Research Award
    David C Warltier
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Anesthesiology 109:371-3. 2008
  5. ncbi request reprint Isoflurane inhibits cardiac myocyte apoptosis during oxidative and inflammatory stress by activating Akt and enhancing Bcl-2 expression
    Marina Jamnicki-Abegg
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesthesiology 103:1006-14. 2005
    ..The underlying mechanisms remain undefined. The authors tested whether isoflurane reduces apoptosis in cardiomyocytes subjected to oxidative or inflammatory stress by enhancing Akt and B-cell lymphoma-2 (Bcl-2)...
  6. ncbi request reprint Characterization of human cardiac mitochondrial ATP-sensitive potassium channel and its regulation by phorbol ester in vitro
    Ming Tao Jiang
    Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 290:H1770-6. 2006
    ..This local PKC control mechanism may represent an alternative pathway to that proposed previously for cytosolic PKC during ischemic/pharmacological preconditioning...
  7. ncbi request reprint Impact of in vivo preconditioning by isoflurane on adenosine triphosphate-sensitive potassium channels in the rat heart: lasting modulation of nucleotide sensitivity during early memory period
    Anna Stadnicka
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesthesiology 104:503-10. 2006
    ....
  8. ncbi request reprint Distinct roles for sarcolemmal and mitochondrial adenosine triphosphate-sensitive potassium channels in isoflurane-induced protection against oxidative stress
    Jasna Marinovic
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226, USA
    Anesthesiology 105:98-104. 2006
    ..However, it is unclear whether K(ATP) channels are triggers initiating the preconditioning signaling, and/or effectors responsible for the cardioprotective memory and activated during ischemia-reperfusion...
  9. ncbi request reprint Mechanism of cardiac sarcolemmal adenosine triphosphate-sensitive potassium channel activation by isoflurane in a heterologous expression system
    Martin Bienengraeber
    Department of Anesthesiology, Medical College of Wisconsin, Wisconsin 53226, USA
    Anesthesiology 105:534-40. 2006
    ..However, it is not known how halogenated anesthetics interact with these channels...
  10. ncbi request reprint Anesthetic preconditioning enhances Ca2+ handling and mechanical and metabolic function elicited by Na+-Ca2+ exchange inhibition in isolated hearts
    Jianzhong An
    Department of Anesthesiology, Medical College of Wisconsin, Wisconsin 53226, USA
    Anesthesiology 105:541-9. 2006
    ..Studies also show the benefit of Na+-Ca2+ exchange inhibition on ischemia-reperfusion injury. The authors tested whether APC plus Na+-Ca2+ exchange inhibitors given just on reperfusion affords additive protection in intact hearts...
  11. ncbi request reprint Chromosomal substitution-dependent differences in cardiovascular responses to sodium pentobarbital
    Thomas A Stekiel
    Department of Anesthesiology, Medical College of Wisconsin, Zablocki Veterans Affairs Medical Center, Milwaukee, Wisconsin 53226, USA
    Anesth Analg 102:799-805. 2006
    ..These results suggest that enhanced cardiovascular sensitivity to PTB in SS rats is related to greater hyperpolarization of VSM transmembrane potential in resistance vessels and this effect is associated with chromosome 13...
  12. ncbi request reprint Isoflurane preconditioning uncouples mitochondria and protects against hypoxia-reoxygenation
    Marko Ljubkovic
    Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Am J Physiol Cell Physiol 292:C1583-90. 2007
    ..In conclusion, isoflurane preconditioning elicits partial mitochondrial uncoupling and reduces mitochondrial Ca(2+) uptake. These effects are likely to reduce the extent of the mitochondrial damage after the hypoxic stress...
  13. ncbi request reprint Volatile anesthetic-induced cardiac preconditioning
    Anna Stadnicka
    Department of Anesthesiology, Medical College of Wisconsin, MEB M4280, 8701 Watertown Plank Road, Milwaukee, Wisconsin 53226, USA
    J Anesth 21:212-9. 2007
    ..Moreover, the article addresses current concepts and controversies regarding the specific roles of the mitochondrial and the sarcolemmal K(ATP) channels in APC...
  14. pmc Differences in cardiovascular sensitivity to propofol in a chromosome substitution rat model
    Thomas A Stekiel
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Croat Med J 48:312-8. 2007
    ....
  15. ncbi request reprint Isoflurane activates human cardiac mitochondrial adenosine triphosphate-sensitive K+ channels reconstituted in lipid bilayers
    Ming T Jiang
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Anesth Analg 105:926-32, table of contents. 2007
    ..In this study, we examined the direct effect of isoflurane and ROS on human cardiac mitoK(ATP) channels reconstituted into the lipid bilayers...
  16. ncbi request reprint Myocardial protection by isoflurane preconditioning preserves Ca2+ cycling proteins independent of sarcolemmal and mitochondrial KATP channels
    Jianzhong An
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesth Analg 105:1207-13, table of contents. 2007
    ..Mitochondrial and sarcolemmal K(ATP) channel openings have been implicated in APC-induced cardioprotection. In this study, we investigated the effect of APC on major calcium cycling proteins and its relation to K(ATP) channels...
  17. doi request reprint Role of sarcolemmal ATP-sensitive potassium channel in oxidative stress-induced apoptosis: mitochondrial connection
    Jasna Marinovic
    Department of Anesthesiology, Medical College of Wisconsin, Miwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 294:H1317-25. 2008
    ....
  18. pmc Age-related attenuation of isoflurane preconditioning in human atrial cardiomyocytes: roles for mitochondrial respiration and sarcolemmal adenosine triphosphate-sensitive potassium channel activity
    Yasushi Mio
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesthesiology 108:612-20. 2008
    ..Here, the impact of age on the role of two major effectors of APC, mitochondria and sarcolemmal adenosine triphosphate-sensitive potassium (sarcKATP) channels, in preconditioning of the human atrial myocardium were investigated...
  19. ncbi request reprint Preconditioning by isoflurane induces lasting sensitization of the cardiac sarcolemmal adenosine triphosphate-sensitive potassium channel by a protein kinase C-delta-mediated mechanism
    Jasna Marinovic
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Anesthesiology 103:540-7. 2005
    ..This study addressed the memory phase of anesthetic preconditioning by investigating whether brief exposure to isoflurane produces lasting sensitization of the sarcKATP channel and whether protein kinase C mediates this effect...
  20. ncbi request reprint The interaction of isoflurane and protein kinase C-activators on sarcolemmal KATP channels
    Lawrence A Turner
    Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Anesth Analg 100:1680-6. 2005
    ....
  21. ncbi request reprint Isoflurane decreases ATP sensitivity of guinea pig cardiac sarcolemmal KATP channel at reduced intracellular pH
    Anna Stadnicka
    Department of Anesthesiology, The Medical College of Wisconsin, Milwaukee 53226, USA
    Anesthesiology 98:396-403. 2003
    ..Because pH is one of the regulators of K(atp) channels, the authors tested the hypothesis that intracellular pH (pHi) modulates the direct interaction of isoflurane with the cardiac K(atp) channel...
  22. ncbi request reprint Repeated or prolonged isoflurane exposure reduces mitochondrial oxidizing effects
    Shinji Kohro
    Departments of Anesthesiology and Anesthesiology Research, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesthesiology 98:275-8. 2003
  23. ncbi request reprint Loss of T-type calcium current in sensory neurons of rats with neuropathic pain
    J Bruce McCallum
    Department of Anesthesiology, Medical College of Wisconsin, Wisconsi 53226, USA
    Anesthesiology 98:209-16. 2003
    ..However, the physiologic function of these channels is unknown. Their possible role in rebound burst firing makes them a candidate for increased excitability after neuropathic injury...
  24. ncbi request reprint Isoflurane sensitizes the cardiac sarcolemmal adenosine triphosphate-sensitive potassium channel to pinacidil
    Susanne Gassmayr
    Department of Anesthesiology double dagger Assistant Professor, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesthesiology 98:114-20. 2003
    ..The authors tested the hypothesis that isoflurane sensitizes sarcK ATP channels to a potassium channel opener, pinacidil, adenosine- and phospholipid-mediated pathways...
  25. ncbi request reprint Biphasic effects of isoflurane on the cardiac action potential: an ionic basis for anesthetic-induced changes in cardiac electrophysiology
    Akihiro Suzuki
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226, USA
    Anesthesiology 97:1209-17. 2002
    ..The results were correlated to modulation of the L-type calcium (I(Ca,L)), the delayed-rectifier potassium (I(Kdr)), and the inward-rectifier potassium (I(Kir)) currents...
  26. ncbi request reprint Protein tyrosine kinase-dependent modulation of isoflurane effects on cardiac sarcolemmal K(ATP) channel
    Anna Stadnicka
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226, USA
    Anesthesiology 97:1198-208. 2002
    ....
  27. ncbi request reprint Isoflurane-induced facilitation of the cardiac sarcolemmal K(ATP) channel
    Kazuhiro Fujimoto
    Department of Anesthesiology, Medical College Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesthesiology 97:57-65. 2002
    ..However, evidence for a direct effect of volatile anesthetic on the K(ATP) channel is limited. In this study, the effects of isoflurane on the cardiac sarcolemmal K(ATP) channel were investigated...
  28. ncbi request reprint Differential modulation of the cardiac adenosine triphosphate-sensitive potassium channel by isoflurane and halothane
    Wai Meng Kwok
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesthesiology 97:50-6. 2002
    ..The goal of this study was to investigate the ability of volatile anesthetics halothane and isoflurane to modulate the cardiac sarcolemmal K(ATP) channel...
  29. ncbi request reprint Isoflurane activates rat mitochondrial ATP-sensitive K+ channels reconstituted in lipid bilayers
    Yuri Nakae
    Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 284:H1865-71. 2003
    ..8 mM (P < 0.05, n = 5 approximately 8). We conclude that direct activation of the mitoK(ATP) channel by isoflurane is likely to contribute to volatile anesthetic-induced myocardial preconditioning...
  30. ncbi request reprint The effects of isoflurane on the cardiac slowly activating delayed-rectifier potassium channel in Guinea pig ventricular myocytes
    Akihiro Suzuki
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226, USA
    Anesth Analg 96:1308-15, table of contents. 2003
    ..Because most of the ion channel studies on anesthetic effects are conducted at room temperature, the temperature-dependent effect on IKs confirms the importance of anesthetic experiments conducted at physiological temperature...
  31. ncbi request reprint Protein kinase C inhibitors produce mitochondrial flavoprotein oxidation in cardiac myocytes
    Shinji Kohro
    Anesthesiology Research, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Anesth Analg 99:1316-22; table of contents. 2004
    ..Instead of a simple mediator, PKC may act as a regulator of the mitoK(ATP) channel to prevent excessive mitochondrial oxidation...
  32. ncbi request reprint Reversal of minimum alveolar concentrations of volatile anesthetics by chromosomal substitution
    Thomas A Stekiel
    Department of Physiology, The Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesthesiology 101:796-8. 2004
  33. ncbi request reprint Antagonists of stretch-activated ion channels restore contractile function in hamster dilated cardiomyopathy
    Alfred C Nicolosi
    Department of Surgery, Division of Cardiothoracic Surgery, Medical College of Wisconsin, 9200 West Wisconsin Avenue, Milwaukee, WI 53226, USA
    J Heart Lung Transplant 23:1003-7. 2004
    ..We hypothesized that SAC antagonists would enhance contractile function in a hamster model of DCM...
  34. ncbi request reprint Protein kinase C-epsilon primes the cardiac sarcolemmal adenosine triphosphate-sensitive potassium channel to modulation by isoflurane
    Kei Aizawa
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Anesthesiology 101:381-9. 2004
    ....
  35. ncbi request reprint Delayed cardioprotection by isoflurane: role of K(ATP) channels
    Marija Tonkovic-Capin
    Division of Pediatric Surgery, Medical College of Wisconsin, Milwaukee, 53226, USA
    Am J Physiol Heart Circ Physiol 283:H61-8. 2002
    ..We conclude that both mitochondrial and sarcolemmal K(ATP) channels contribute to isoflurane-induced delayed cardioprotection...
  36. ncbi request reprint Contribution of reactive oxygen species to isoflurane-induced sensitization of cardiac sarcolemmal adenosine triphosphate-sensitive potassium channel to pinacidil
    Jianzhong An
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226, USA
    Anesthesiology 100:575-80. 2004
    ..Because reactive oxygen species seem to be mediators in anesthetic preconditioning, the authors investigated whether they contribute to the mechanism of the sensitization effect by isoflurane...
  37. ncbi request reprint Intracellular mechanism of mitochondrial adenosine triphosphate-sensitive potassium channel activation with isoflurane
    Yuri Nakae
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee 53226, USA
    Anesth Analg 97:1025-32, table of contents. 2003
    ..However, our data do not support an action of isoflurane through pathways involving adenosine, PKC, tyrosine kinase, or mitogen-activated protein kinase. These results suggest that isoflurane may directly activate mitoK(ATP)...
  38. ncbi request reprint Reactive oxygen species precede the epsilon isoform of protein kinase C in the anesthetic preconditioning signaling cascade
    Enis Novalija
    Department of Anesthesiology, Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesthesiology 99:421-8. 2003
    ..The authors aimed to identify the PKC isoform that is involved in APC and to elucidate the relative positions of PKC activation and ROS formation in the APC signaling cascade...
  39. pmc Targeted expression of Kir6.2 in mitochondria confers protection against hypoxic stress
    Marko Ljubkovic
    Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Physiol 577:17-29. 2006
    ..In conclusion, this study provides the first non-pharmacological evidence that an increased K(+) influx to mitochondria protects against hypoxic stress by preventing detrimental effects of Ca(2+) overload...
  40. ncbi request reprint 2007 in review: a dozen steps forward in Anesthesiology
    James C Eisenach
    Department of Anesthesiology, Wake Forest University School of Medicine, Winston Salem, North Carolina, USA
    Anesthesiology 108:149-55. 2008

Research Grants27

  1. ANESTHETICS AND CARDIAC SIGNAL TRANSDUCTION
    Zeljko Bosnjak; Fiscal Year: 2003
    ..3) To characterize the effect of VA on cloned KATP channels expressed in HEK293 cells. The hypothesis tested is that the effect of VA on expressed KATP channels is attenuated compared to their effect on native KATP channels. ..
  2. ANESTHETIC-INDUCED CARDIAC PRECONDITIONING
    Zeljko Bosnjak; Fiscal Year: 2007
    ..Core Directors: Meetha Medhora. Ph.D. and David Harder. Ph.D. ..
  3. ANESTHETICS AND CARDIAC SIGNAL TRANSDUCTION
    Zeljko Bosnjak; Fiscal Year: 2009
    ..This proposal represents a comprehensive approach to the significant and clinically relevant phenomenon of volatile anesthetic-induced cardioprotection against ischemia/reperfusion injury. ..
  4. INTERACTION BETWEEN ANESTHETICS AND CALCIUM ANTAGONISTS
    Zeljko Bosnjak; Fiscal Year: 1993
    ..These studies are designed to better understand the clinically observed cardiac effects of volatile anesthetics and calcium antagonists...
  5. ANESTHETICS AND CARDIAC SIGNAL TRANSDUCTION
    Zeljko Bosnjak; Fiscal Year: 1999
    ..The proposed studies of this application are coordinated as a major effort to understand the mechanisms by which anesthetics in combination with other drugs modulate cardiac electrophysiology. ..
  6. ANESTHETICS AND CARDIAC SIGNAL TRANSDUCTION
    Zeljko J Bosnjak; Fiscal Year: 2010
    ..Defects observed during our study may then be targeted via various therapies. ..