JOSEPH BESHARSE

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. pmc The de-ubiquitinylating enzyme, USP2, is associated with the circadian clockwork and regulates its sensitivity to light
    Heather Dehlin Scoma
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America
    PLoS ONE 6:e25382. 2011
  2. pmc Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development
    Christine Insinna
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 0509, USA
    Neural Dev 5:12. 2010
  3. pmc Rhythmic expression of Nocturnin mRNA in multiple tissues of the mouse
    Y Wang
    PEL FREEZ Clinical Systems, LLC, 9099 North Deerbrook Trail, Brown Deer, WI 53223, USA
    BMC Dev Biol 1:9. 2001
  4. ncbi request reprint Regulation of photoreceptor Per1 and Per2 by light, dopamine and a circadian clock
    Joseph C Besharse
    Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Eur J Neurosci 20:167-74. 2004
  5. ncbi request reprint Photoreceptor intersegmental transport and retinal degeneration: a conserved pathway common to motile and sensory cilia
    Joseph C Besharse
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Adv Exp Med Biol 533:157-64. 2003
  6. ncbi request reprint Visualization of identified GFP-expressing cells by light and electron microscopy
    Katherine Luby-Phelps
    Department of Cell Biology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Histochem Cytochem 51:271-4. 2003
  7. ncbi request reprint IFT20 links kinesin II with a mammalian intraflagellar transport complex that is conserved in motile flagella and sensory cilia
    Sheila A Baker
    Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 278:34211-8. 2003
  8. doi request reprint Analysis of IFT kinesins in developing zebrafish cone photoreceptor sensory cilia
    Christine Insinna
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 0509, USA
    Methods Cell Biol 93:219-34. 2009
  9. pmc The homodimeric kinesin, Kif17, is essential for vertebrate photoreceptor sensory outer segment development
    Christine Insinna
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Dev Biol 316:160-70. 2008
  10. pmc Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors
    Katherine Luby-Phelps
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 0509, USA
    Vision Res 48:413-23. 2008

Research Grants

  1. VISUAL CELL PIGMENT CELL INTERFACE AND DISC TURNOVER
    JOSEPH BESHARSE; Fiscal Year: 2005
  2. VISUAL CELL PIGMENT CELL INTERFACE AND DISC TURNOVER
    Joseph C Besharse; Fiscal Year: 2010
  3. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 2005
  4. Rresearch Training Program in Vision Science
    JOSEPH BESHARSE; Fiscal Year: 2005
  5. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 2006
  6. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 2007
  7. FASEB Summer Research Conference on "The Biology and Chemistry of Vision"
    JOSEPH BESHARSE; Fiscal Year: 2007
  8. VISUAL CELL PIGMENT CELL INTERFACE AND DISC TURNOVER
    JOSEPH BESHARSE; Fiscal Year: 2009
  9. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 2004
  10. VISUAL CELL PIGMENT CELL INTERFACE AND DISC TURNOVER
    JOSEPH BESHARSE; Fiscal Year: 2004

Collaborators

Detail Information

Publications19

  1. pmc The de-ubiquitinylating enzyme, USP2, is associated with the circadian clockwork and regulates its sensitivity to light
    Heather Dehlin Scoma
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America
    PLoS ONE 6:e25382. 2011
    ..Rhythmic expression of USP2 in the SCN and other tissues offers a new level of control of the clock machinery through de-ubiqutinylation and suggests a role for USP2 during circadian adaptation to environmental day length changes...
  2. pmc Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development
    Christine Insinna
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 0509, USA
    Neural Dev 5:12. 2010
    ..Proper morphogenesis of photoreceptor neurons is essential for their normal function and survival. We have characterized a zebrafish mutation, cannonball, that completely disrupts photoreceptor morphogenesis...
  3. pmc Rhythmic expression of Nocturnin mRNA in multiple tissues of the mouse
    Y Wang
    PEL FREEZ Clinical Systems, LLC, 9099 North Deerbrook Trail, Brown Deer, WI 53223, USA
    BMC Dev Biol 1:9. 2001
    ..The goal of this study was to determine the temporal pattern of nocturnin mRNA expression in multiple tissues of the mouse...
  4. ncbi request reprint Regulation of photoreceptor Per1 and Per2 by light, dopamine and a circadian clock
    Joseph C Besharse
    Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Eur J Neurosci 20:167-74. 2004
    ....
  5. ncbi request reprint Photoreceptor intersegmental transport and retinal degeneration: a conserved pathway common to motile and sensory cilia
    Joseph C Besharse
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Adv Exp Med Biol 533:157-64. 2003
  6. ncbi request reprint Visualization of identified GFP-expressing cells by light and electron microscopy
    Katherine Luby-Phelps
    Department of Cell Biology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Histochem Cytochem 51:271-4. 2003
    ..Furthermore, semithin sections can be used to locate GFP-expressing cells for examination by immunoelectron microscopy of the same material after thin sectioning...
  7. ncbi request reprint IFT20 links kinesin II with a mammalian intraflagellar transport complex that is conserved in motile flagella and sensory cilia
    Sheila A Baker
    Department of Cell Biology, Neurobiology, and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Biol Chem 278:34211-8. 2003
    ..Our data indicate that all four mammalian IFT proteins are part of a highly conserved complex in multiple ciliated cell types. Furthermore, IFT20 appears to bridge kinesin II with the IFT complex...
  8. doi request reprint Analysis of IFT kinesins in developing zebrafish cone photoreceptor sensory cilia
    Christine Insinna
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 0509, USA
    Methods Cell Biol 93:219-34. 2009
    ....
  9. pmc The homodimeric kinesin, Kif17, is essential for vertebrate photoreceptor sensory outer segment development
    Christine Insinna
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Dev Biol 316:160-70. 2008
    ..Our analysis shows that Kif17 is essential for photoreceptor OS development, and suggests that Kif17 plays a cell type specific role in vertebrate ciliogenesis...
  10. pmc Spatial distribution of intraflagellar transport proteins in vertebrate photoreceptors
    Katherine Luby-Phelps
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 0509, USA
    Vision Res 48:413-23. 2008
    ..IFT52-GFP and IFT57-GFP mimicked this pattern in transgenic Xenopus...
  11. pmc Different roles for KIF17 and kinesin II in photoreceptor development and maintenance
    Christine Insinna
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Dev Dyn 238:2211-22. 2009
    ..In contrast, dominant-negative KIF17 has no obvious effect on inner segment or synaptic organization but has an immediate impact on outer segment assembly...
  12. pmc The intraflagellar transport protein, IFT88, is essential for vertebrate photoreceptor assembly and maintenance
    Gregory J Pazour
    Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Cell Biol 157:103-13. 2002
    ..We further find that mice with a mutation in the IFT particle protein gene, Tg737/IFT88, have abnormal OS development and retinal degeneration. Thus, IFT is important for assembly and maintenance of the vertebrate OS...
  13. pmc Intraflagellar transport and the sensory outer segment of vertebrate photoreceptors
    Christine Insinna
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, Wisconsin 53226 0509, USA
    Dev Dyn 237:1982-92. 2008
    ..Those features illustrate how further analysis of photoreceptor IFT may provide insight into both IFT cargo and the role of alternative IFT kinesins...
  14. ncbi request reprint Implications of circadian gene expression in kidney, liver and the effects of fasting on pharmacogenomic studies
    Yasuhiro Kita
    Department of Physiology, Medical College of Wisconsin, Milwaukee, WI 53228, USA
    Pharmacogenetics 12:55-65. 2002
    ..It also identifies 18 new genes that should be investigated for a role in circadian rhythms in peripheral tissues...
  15. ncbi request reprint Retinal circadian clocks and control of retinal physiology
    Carla B Green
    Department of Biology, University of Virginia, Charlottesville, VA 22904, USA
    J Biol Rhythms 19:91-102. 2004
    ..Finally, the interactions between the retinal clock(s) and the master clock in the SCN are examined...
  16. pmc Loss of Nocturnin, a circadian deadenylase, confers resistance to hepatic steatosis and diet-induced obesity
    Carla B Green
    Department of Biology, University of Virginia, Charlottesville, VA 22904, USA
    Proc Natl Acad Sci U S A 104:9888-93. 2007
    ..Our data support a pivotal role for Nocturnin downstream of the circadian clockwork in the posttranscriptional regulation of genes necessary for nutrient uptake, metabolism, and storage...
  17. pmc Immediate early response of the circadian polyA ribonuclease nocturnin to two extracellular stimuli
    Eduardo Garbarino-Pico
    Department of Biology, University of Virginia, Charlottesville, Virginia 22904, USA
    RNA 13:745-55. 2007
    ..NOC may act in turning off the expression of genes that are required to be silenced as a response to these extracellular signals...
  18. ncbi request reprint Quantification of the cytoplasmic spaces of living cells with EGFP reveals arrestin-EGFP to be in disequilibrium in dark adapted rod photoreceptors
    Jon A Peet
    Temple University School of Medicine, 3400 North Broad Street, Philadelphia, PA 19140, USA
    J Cell Sci 117:3049-59. 2004
    ..Arrestin-EGFP mass was conserved during a large-scale, light-driven redistribution in which approximately 40% of the protein in the inner segment moved to the outer segment in less than 30 minutes...
  19. ncbi request reprint Molecular structure of cytoplasmic dynein 2 and its distribution in neuronal and ciliated cells
    Atsushi Mikami
    Department of Pathology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA
    J Cell Sci 115:4801-8. 2002
    ..These data support a specific role for dynein 2 in the generation and maintenance of cilia...

Research Grants27

  1. VISUAL CELL PIGMENT CELL INTERFACE AND DISC TURNOVER
    JOSEPH BESHARSE; Fiscal Year: 2005
    ....
  2. VISUAL CELL PIGMENT CELL INTERFACE AND DISC TURNOVER
    Joseph C Besharse; Fiscal Year: 2010
    ..The goal of this research project is to determine the mechanism of targeting of a protein (membrane guanylyl cyclase) that when disrupted genetically leads to severe photoreceptor degeneration and blindness. ..
  3. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 2005
    ..This project fills specific gaps in our knowledge of retinal circadian organization at the cellular level in a mammalian system, and assesses the potential effects of circadian gene disruption on retinal health. ..
  4. Rresearch Training Program in Vision Science
    JOSEPH BESHARSE; Fiscal Year: 2005
    ..They will also attend and present papers at National meetings. ..
  5. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 2006
    ..This project fills specific gaps in our knowledge of retinal circadian organization at the cellular level in a mammalian system, and assesses the potential effects of circadian gene disruption on retinal health. ..
  6. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 2007
    ..This project fills specific gaps in our knowledge of retinal circadian organization at the cellular level in a mammalian system, and assesses the potential effects of circadian gene disruption on retinal health. ..
  7. FASEB Summer Research Conference on "The Biology and Chemistry of Vision"
    JOSEPH BESHARSE; Fiscal Year: 2007
    ..The funding requested in this application will be used to support travel and conference expenses for pre- and post-doctoral trainees, and entry level scientists that have not yet established their own research programs. ..
  8. VISUAL CELL PIGMENT CELL INTERFACE AND DISC TURNOVER
    JOSEPH BESHARSE; Fiscal Year: 2009
    ..The goal of this research project is to determine the mechanism of targeting of a protein (membrane guanylyl cyclase) that when disrupted genetically leads to severe photoreceptor degeneration and blindness. ..
  9. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 2004
    ..Disruption of these interactions in constant light is proposed to account for disruption of circadian rhythms and for known alterations in photoreceptor membrane turnover. ..
  10. VISUAL CELL PIGMENT CELL INTERFACE AND DISC TURNOVER
    JOSEPH BESHARSE; Fiscal Year: 2004
    ....
  11. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 2003
    ..Disruption of these interactions in constant light is proposed to account for disruption of circadian rhythms and for known alterations in photoreceptor membrane turnover. ..
  12. VISUAL CELL PIGMENT CELL INTERFACE AND DISC TURNOVER
    JOSEPH BESHARSE; Fiscal Year: 1999
    ..Specific Aim 3. To characterize kinesin-like proteins in photoreceptor ciliary axonemes with emphasis on their location and potential role as a transport motor. ..
  13. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 1999
    ....
  14. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 2000
    ....
  15. VISUAL CELL PIGMENT CELL INTERFACE AND DISC TURNOVER
    JOSEPH BESHARSE; Fiscal Year: 2001
    ....
  16. LEICA TCS SPII SPECTRAL CONFOCAL MICROSCOPE
    JOSEPH BESHARSE; Fiscal Year: 2001
    ..After the project period, the long term care of the instrument for use of NIH funded investigators will be placed under the supervision of the existing imaging core facility. ..
  17. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 2001
    ..Disruption of these interactions in constant light is proposed to account for disruption of circadian rhythms and for known alterations in photoreceptor membrane turnover. ..
  18. VISUAL CELL PIGMENT CELL INTERFACE AND DISC TURNOVER
    JOSEPH BESHARSE; Fiscal Year: 2002
    ....
  19. DISK RENEWAL IN RETINAL VISUAL CELLS
    JOSEPH BESHARSE; Fiscal Year: 2002
    ..Disruption of these interactions in constant light is proposed to account for disruption of circadian rhythms and for known alterations in photoreceptor membrane turnover. ..
  20. VISUAL CELL PIGMENT CELL INTERFACE AND DISC TURNOVER
    JOSEPH BESHARSE; Fiscal Year: 2003
    ....