John Auchampach

Summary

Affiliation: Medical College of Wisconsin
Country: USA

Publications

  1. pmc Polyamidoamine (PAMAM) dendrimer conjugate specifically activates the A3 adenosine receptor to improve post-ischemic/reperfusion function in isolated mouse hearts
    Tina C Wan
    Department of Pharmacology Toxicology and the Cardiovascular Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    BMC Pharmacol 11:11. 2011
  2. pmc Protection from myocardial ischemia/reperfusion injury by a positive allosteric modulator of the A₃ adenosine receptor
    Lili Du
    Department of Pharmacology and the Cardiovascular Center, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    J Pharmacol Exp Ther 340:210-7. 2012
  3. pmc Synthesis and pharmacological characterization of [(125)I]MRS5127, a high affinity, selective agonist radioligand for the A3 adenosine receptor
    John A Auchampach
    Department of Pharmacology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, United States
    Biochem Pharmacol 79:967-73. 2010
  4. ncbi request reprint A3 adenosine receptor agonist IB-MECA reduces myocardial ischemia-reperfusion injury in dogs
    John A Auchampach
    Cardiovascular Research Center, Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 285:H607-13. 2003
  5. pmc Comparison of three different A1 adenosine receptor antagonists on infarct size and multiple cycle ischemic preconditioning in anesthetized dogs
    John A Auchampach
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 308:846-56. 2004
  6. pmc Characterization of the A2B adenosine receptor from mouse, rabbit, and dog
    John A Auchampach
    Department of Pharmacology and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 329:2-13. 2009
  7. pmc The A3 adenosine receptor agonist CP-532,903 [N6-(2,5-dichlorobenzyl)-3'-aminoadenosine-5'-N-methylcarboxamide] protects against myocardial ischemia/reperfusion injury via the sarcolemmal ATP-sensitive potassium channel
    Tina C Wan
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 324:234-43. 2008
  8. pmc Cl-IB-MECA [2-chloro-N6-(3-iodobenzyl)adenosine-5'-N-methylcarboxamide] reduces ischemia/reperfusion injury in mice by activating the A3 adenosine receptor
    Zhi Dong Ge
    Department of Pharmacology and Toxicology and the Cardiovascular Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Pharmacol Exp Ther 319:1200-10. 2006
  9. pmc Adenosine inhibits tumor necrosis factor-alpha release from mouse peritoneal macrophages via A2A and A2B but not the A3 adenosine receptor
    Laura M Kreckler
    Department of Pharmacology, Medical College of Wisconsin, Milwaukee, 53226, USA
    J Pharmacol Exp Ther 317:172-80. 2006
  10. pmc A(3) adenosine receptor activation during reperfusion reduces infarct size through actions on bone marrow-derived cells
    Zhi Dong Ge
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Mol Cell Cardiol 49:280-6. 2010

Research Grants

  1. A3 Adenosine Receptors Cardioprotective Mechanisms
    John Auchampach; Fiscal Year: 2007
  2. A3 RECEPTORS--CARDIOPROTECTIVE MECHANISMS
    John Auchampach; Fiscal Year: 2003
  3. Role of Adenosine Receptors in Tissue Protection
    John Auchampach; Fiscal Year: 2009

Collaborators

Detail Information

Publications25

  1. pmc Polyamidoamine (PAMAM) dendrimer conjugate specifically activates the A3 adenosine receptor to improve post-ischemic/reperfusion function in isolated mouse hearts
    Tina C Wan
    Department of Pharmacology Toxicology and the Cardiovascular Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    BMC Pharmacol 11:11. 2011
    ..We have examined pharmacologically the protective properties of a multivalent dendrimeric conjugate of a nucleoside as a selective multivalent agonist for the mouse A3AR...
  2. pmc Protection from myocardial ischemia/reperfusion injury by a positive allosteric modulator of the A₃ adenosine receptor
    Lili Du
    Department of Pharmacology and the Cardiovascular Center, Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    J Pharmacol Exp Ther 340:210-7. 2012
    ..This is the first study to demonstrate efficacy of an A₃ AR allosteric enhancer in an in vivo model of infarction...
  3. pmc Synthesis and pharmacological characterization of [(125)I]MRS5127, a high affinity, selective agonist radioligand for the A3 adenosine receptor
    John A Auchampach
    Department of Pharmacology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, United States
    Biochem Pharmacol 79:967-73. 2010
    ..The major advantage of [(125)I]MRS5127 compared with previously used A(3)AR radioligands is its high affinity, low degree of non-specific binding, and improved A(3)AR selectivity...
  4. ncbi request reprint A3 adenosine receptor agonist IB-MECA reduces myocardial ischemia-reperfusion injury in dogs
    John A Auchampach
    Cardiovascular Research Center, Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Rd, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 285:H607-13. 2003
    ..These data also demonstrate in an in vivo model that IB-MECA is effective as a cardioprotective agent when administered at the time of reperfusion...
  5. pmc Comparison of three different A1 adenosine receptor antagonists on infarct size and multiple cycle ischemic preconditioning in anesthetized dogs
    John A Auchampach
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 308:846-56. 2004
    ..We discuss the possibility that the cardioprotective actions of CPX and BG 9928 may be related to antagonism of A(2B)ARs...
  6. pmc Characterization of the A2B adenosine receptor from mouse, rabbit, and dog
    John A Auchampach
    Department of Pharmacology and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 329:2-13. 2009
    ..We conclude that significant differences in antagonist pharmacology of the A(2B)AR exist between species and that some species express nonfunctional variants of the A(2B)AR due to "leaky" splicing...
  7. pmc The A3 adenosine receptor agonist CP-532,903 [N6-(2,5-dichlorobenzyl)-3'-aminoadenosine-5'-N-methylcarboxamide] protects against myocardial ischemia/reperfusion injury via the sarcolemmal ATP-sensitive potassium channel
    Tina C Wan
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Pharmacol Exp Ther 324:234-43. 2008
    ..We conclude that CP-532,903 is a highly selective agonist of the mouse A(3)AR that protects against ischemia/reperfusion injury by activating sarcolemmal K(ATP) channels...
  8. pmc Cl-IB-MECA [2-chloro-N6-(3-iodobenzyl)adenosine-5'-N-methylcarboxamide] reduces ischemia/reperfusion injury in mice by activating the A3 adenosine receptor
    Zhi Dong Ge
    Department of Pharmacology and Toxicology and the Cardiovascular Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Pharmacol Exp Ther 319:1200-10. 2006
    ..These data demonstrate that Cl-IB-MECA protects against myocardial ischemia/reperfusion injury in mice principally by activating the A(3)AR...
  9. pmc Adenosine inhibits tumor necrosis factor-alpha release from mouse peritoneal macrophages via A2A and A2B but not the A3 adenosine receptor
    Laura M Kreckler
    Department of Pharmacology, Medical College of Wisconsin, Milwaukee, 53226, USA
    J Pharmacol Exp Ther 317:172-80. 2006
    ....
  10. pmc A(3) adenosine receptor activation during reperfusion reduces infarct size through actions on bone marrow-derived cells
    Zhi Dong Ge
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    J Mol Cell Cardiol 49:280-6. 2010
    ....
  11. pmc Gene dosage-dependent effects of cardiac-specific overexpression of the A3 adenosine receptor
    Richard G Black
    Department of Pharmacology and Toxicology and the Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee, Wis 53226, USA
    Circ Res 91:165-72. 2002
    ....
  12. pmc Activation of the A(3) adenosine receptor suppresses superoxide production and chemotaxis of mouse bone marrow neutrophils
    Dharini van der Hoeven
    Department of Pharmacology and Cardiovascular Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Mol Pharmacol 74:685-96. 2008
    ..e., inhibition of neutrophil-mediated tissue injury)...
  13. ncbi request reprint Extending the cardioprotective window using a novel delta-opioid agonist fentanyl isothiocyanate via the PI3-kinase pathway
    Eric R Gross
    Medical College of Wisconsin, Dept of Pharmacology and Toxicology, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    Am J Physiol Heart Circ Physiol 288:H2744-9. 2005
    ..These data suggest that FIT has a prolonged cardioprotective window greater than that of any previously described cardioprotective agent that requires PI3K primarily in the trigger phase but also partially, as a mediator or end effector...
  14. ncbi request reprint Therapeutic receptor targets of ischemic preconditioning
    Ryan M Fryer
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee 53226, USA
    Cardiovasc Res 55:520-5. 2002
    ..This is not meant to completely rule out other receptors since it is clear that IPC is a phenomenon with multiple pathways that appear to be responsible for the cardioprotection observed...
  15. doi request reprint Isoflurane postconditioning protects against reperfusion injury by preventing mitochondrial permeability transition by an endothelial nitric oxide synthase-dependent mechanism
    Zhi Dong Ge
    Department of Anesthesiology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    Anesthesiology 112:73-85. 2010
    ..The role of endothelial nitric oxide synthase (eNOS) in isoflurane postconditioning (IsoPC)-elicited cardioprotection is poorly understood. The authors addressed this issue using eNOS mice...
  16. pmc Reperfusion injury: does it exist?
    Garrett J Gross
    Medical College of Wisconsin, Department of Pharmacology and Toxicology, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA
    J Mol Cell Cardiol 42:12-8. 2007
    ....
  17. pmc Adenosine suppresses lipopolysaccharide-induced tumor necrosis factor-alpha production by murine macrophages through a protein kinase A- and exchange protein activated by cAMP-independent signaling pathway
    Laura M Kreckler
    Department of Pharmacology and the Cardiovascular Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Pharmacol Exp Ther 331:1051-61. 2009
    ....
  18. pmc Improved cardiac function in infarcted mice after treatment with pluripotent embryonic stem cells
    Timothy J Nelson
    Department of Cell Biology, Neurobiology, and Anatomy and the Cardiovascular Center Medical College of Wisconsin, Milwaukee, Wisconsin, USA
    Anat Rec A Discov Mol Cell Evol Biol 288:1216-24. 2006
    ..These experiments indicate that the infarcted myocardial environment can support engraftment and cardiomyogenic differentiation of pluripotent ESCs, concomitant with partial functional recovery...
  19. ncbi request reprint Comparison of cardioprotective efficacy of two thromboxane A2 receptor antagonists
    Zhi Dong Ge
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Cardiovasc Pharmacol 41:481-8. 2003
    ..These results suggest that the beneficial effects of KT2-962 may be due to its direct free radical scavenging properties rather than its ability to block TXA receptors.(2) (2) (2)..
  20. pmc Inhibition of an equilibrative nucleoside transporter by cannabidiol: a mechanism of cannabinoid immunosuppression
    Erica J Carrier
    Department of Pharmacology and Toxicology, Medical College of Wisconsin, Milwaukee, WI 53226, USA
    Proc Natl Acad Sci U S A 103:7895-900. 2006
    ..These studies demonstrate that CBD has the ability to enhance adenosine signaling through inhibition of uptake and provide a non-cannabinoid receptor mechanism by which CBD can decrease inflammation...
  21. pmc Activation of the A(3) adenosine receptor inhibits fMLP-induced Rac activation in mouse bone marrow neutrophils
    Dharini van der Hoeven
    Department of Pharmacology and Toxicology and the Cardiovascular Center, Medical College of Wisconsin, Milwaukee, 53226, United States
    Biochem Pharmacol 79:1667-73. 2010
    ..Our results suggest that activation of the A(3)AR signals to suppress neutrophil functions by interfering with the monomeric GTPase Rac, thus contributing to the ant-inflammatory actions of adenosine...
  22. pmc A role for the low-affinity A2B adenosine receptor in regulating superoxide generation by murine neutrophils
    Dharini van der Hoeven
    Department of Pharmacology and the Cardiovascular Center, Medical College of Wisconsin, Milwaukee, Wisconsin 53226, USA
    J Pharmacol Exp Ther 338:1004-12. 2011
    ....
  23. pmc Adenosine receptors and angiogenesis
    John A Auchampach
    Circ Res 101:1075-7. 2007
  24. pmc Design of (N)-methanocarba adenosine 5'-uronamides as species-independent A3 receptor-selective agonists
    Artem Melman
    Molecular Recognition Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Building 8A, Room B1A 19, Bethesda, MD 20892 0810, USA
    Bioorg Med Chem Lett 18:2813-9. 2008
    ..Extended and chemically functionalized alkynyl chains attached at the C2 position of the purine moiety preserved A(3)AR selectivity more effectively than similar chains attached at the 3-position of the N(6)-benzyl group...
  25. ncbi request reprint Gene dose-dependent atrial arrhythmias, heart block, and brady-cardiomyopathy in mice overexpressing A(3) adenosine receptors
    Larissa Fabritz
    Department of Cardiology and Angiology and Institute for Arteriosclerosis Research, University Hospital Munster, Muenster, Germany
    Cardiovasc Res 62:500-8. 2004
    ..We therefore studied the effects of heart-directed overexpression of A(3) adenosine receptors (A(3)ARs) at different gene doses on sinus and atrio-ventricular (AV) nodal function in mice...

Research Grants12

  1. A3 Adenosine Receptors Cardioprotective Mechanisms
    John Auchampach; Fiscal Year: 2007
    ..The results of theses studies should also provide new insights into the therapeutic utility of targeting A3ARs in patients with ischemic heart disease. ..
  2. A3 RECEPTORS--CARDIOPROTECTIVE MECHANISMS
    John Auchampach; Fiscal Year: 2003
    ....
  3. Role of Adenosine Receptors in Tissue Protection
    John Auchampach; Fiscal Year: 2009
    ..Collectively, these studies will combine several state-of-the-art techniques to provide new definitive information on the cell-specific mechanisms by which A3AR activation provides protection from I/R injury. ..