Arshak Alexanian

Summary

Affiliation: Medical College of Wisconsin
Location: Milwaukee, USA
URL: http://www.mcw.edu/neurosurgery/faculty/Non-SurgFaculty/alexanian.htm
Summary:
The main focus of my current research is to understand the genetic and epigenetic mechanisms governing cell plasticity (proliferation, lineage commitment and differentiation) and application of these findings for regenerative medicine
Publications:
1.Transplanted neurally modified bone marrow-derived mesenchymal stem cells promote tissue protection and locomotor recovery in spinal cord injured rats.
Alexanian AR, Fehlings MG, Zhang Z, Maiman DJ.
Neurorehabil Neural Repair. 2011 Nov;25(9):873-80. Epub 2011 Aug 15.
PMID:[PubMed - in process]
Related citations

2.Feline bone marrow-derived mesenchymal stem cells express several pluripotent and neural markers and easily turn into neural-like cells by manipulation with chromatin modifying agents and neural inducing factors.
Zhang Z, Maiman DJ, Kurpad SN, Crowe MJ, Alexanian AR.
Cell Reprogram. 2011 Oct;13(5):385-90. Epub 2011 Jun 30.
PMID:[PubMed - in process]
Related citations

3.Survival of neurally induced mesenchymal cells may determine degree of motor recovery in injured spinal cord rats.
Alexanian AR, Kwok WM, Pravdic D, Maiman DJ, Fehlings MG.
Restor Neurol Neurosci. 2010;28(6):761-7.
PMID:[PubMed - indexed for MEDLINE]
Related citations

4.An efficient method for generation of neural-like cells from adult human bone marrow-derived mesenchymal stem cells.
Alexanian AR.
Regen Med. 2010 Nov;5(6):891-900.
PMID:[PubMed - indexed for MEDLINE]
Related citations

5.Transplantation of human glial-restricted neural precursors into injured spinal cord promotes functional and sensory recovery without causing allodynia.
Alexanian AR, Svendsen CN, Crowe MJ, Kurpad SN.
Cytotherapy. 2011 Jan;13(1):61-8. Epub 2010 Aug 24.
PMID:[PubMed - indexed for MEDLINE]
Related citations

6.In vitro and in vivo characterization of neurally modified mesenchymal stem cells induced by epigenetic modifiers and neural stem cell environment.
Alexanian AR, Maiman DJ, Kurpad SN, Gennarelli TA.
Stem Cells Dev. 2008 Dec;17(6):1123-30.
PMID:[PubMed - indexed for MEDLINE]
Related citations

7.Epigenetic modifiers promote efficient generation of neural-like cells from bone marrow-derived mesenchymal cells grown in neural environment.
Alexanian AR.
J Cell Biochem. 2007 Feb 1;100(2):362-71.
PMID:[PubMed - indexed for MEDLINE]
Related citations

8.Pain with no gain: allodynia following neural stem cell transplantation in spinal cord injury.
Macias MY, Syring MB, Pizzi MA, Crowe MJ, Alexanian AR, Kurpad SN.
Exp Neurol. 2006 Oct;201(2):335-48. Epub 2006 Jul 12.
PMID:[PubMed - indexed for MEDLINE]
Related citations

9.Neural stem cells induce bone-marrow-derived mesenchymal stem cells to generate neural stem-like cells via juxtacrine and paracrine interactions.
Alexanian AR.
Exp Cell Res. 2005 Nov 1;310(2):383-91. Epub 2005 Oct 5.
PMID:[PubMed - indexed for MEDLINE]
Related citations

10.Efficient differentiation and integration of lineage-restricted neural precursors in the traumatically injured adult cat spinal cord.
Alexanian AR, Crowe MJ, Kurpad SN.
J Neurosci Methods. 2006 Jan 15;150(1):41-6. Epub 2005 Aug 8.
PMID:[PubMed - indexed for MEDLINE]
Related citations

11.Quiescent neural cells regain multipotent stem cell characteristics influenced by adult neural stem cells in co-culture.
Alexanian AR, Kurpad SN.
Exp Neurol. 2005 Jan;191(1):193-7.
PMID:[PubMed - indexed for MEDLINE]
Related citations

12.Differentiating adult hippocampal stem cells into neural crest derivatives.
Alexanian AR, Sieber-Blum M.
Neuroscience. 2003;118(1):1-5.
PMID:[PubMed - indexed for MEDLINE]
Related citations

13.Calcium-dependent regulation of interactions of caldesmon with calcium-binding proteins found in growth cones of chick forebrain neurons.
Alexanian AR, Bamburg JR, Hidaka H, Mornet D.
Cell Mol Neurobiol. 2001 Oct;21(5):437-51.
PMID:[PubMed - indexed for MEDLINE]
Related citations

14.Proliferation and regeneration of retrogradely labeled adult rat corticospinal neurons in culture.
Alexanian AR, Nornes HO.
Exp Neurol. 2001 Aug;170(2):277-82.
PMID:[PubMed - indexed for MEDLINE]
Related citations

15.Reversal of drug resistance in sarcoma-45 by the new calmodulin antagonist--trihydrochloride of [1,2,5-trimethyl-4-phenyl-4-beta-[N-(beta-ethylamino)-N-4-methoxybe nzy l]-ethylamino] piperidine (AR-2).
Alexanian AR, Arutyunian NS.
Invest New Drugs. 1999;17(2):105-10.
PMID:[PubMed - indexed for MEDLINE]
Related citations

16.Neuronal survival activity of s100betabeta is enhanced by calcineurin inhibitors and requires activation of NF-kappaB.
Alexanian AR, Bamburg JR.
FASEB J. 1999 Sep;13(12):1611-20.
PMID:[PubMed - indexed for MEDLINE] Free Article
Related citations

17.Antiarrhythmic action of the new calcium antagonist [1,2,5-trimethyl-4-phenyl-4-beta-(N-cyanoethyl-N-4-methoxybenzyl) -ethylamino]piperidine dihydrochloride.
Alexanian AR, Arutyunian NS, Galoyan AA.
Arzneimittelforschung. 1996 Dec;46(12):1110-3.
PMID:9006783[PubMed - indexed for MEDLINE]
Related citations

Publications

  1. request reprint
    Macias M, Syring M, Pizzi M, Crowe M, Alexanian A, Kurpad S. Pain with no gain: allodynia following neural stem cell transplantation in spinal cord injury. Exp Neurol. 2006;201:335-48 pubmed
    ..Both allodynia- and CGRP/GAP43-positive afferent sprouting were less in the C17.2/GDNF group compared to the C17.2 group, suggesting a possible protective or analgesic effect of GDNF on post-injury neuropathic pain. ..
  2. request reprint
    Alexanian A, Bamburg J. Neuronal survival activity of s100betabeta is enhanced by calcineurin inhibitors and requires activation of NF-kappaB. FASEB J. 1999;13:1611-20 pubmed
    ..We conclude that S100betabeta enhances the survival of chick embryo forebrain neurons through the activation of NF-kappaB. ..
  3. Alexanian A, Fehlings M, Zhang Z, Maiman D. Transplanted neurally modified bone marrow-derived mesenchymal stem cells promote tissue protection and locomotor recovery in spinal cord injured rats. Neurorehabil Neural Repair. 2011;25:873-80 pubmed publisher
    ..Transplantation also reduced the volume of lesion cavity and white matter loss. This method of hMSC modification may provide an alternative source of autologous adult stem cells for CNS repair. ..
  4. request reprint
    Alexanian A, Bamburg J, Hidaka H, Mornet D. Calcium-dependent regulation of interactions of caldesmon with calcium-binding proteins found in growth cones of chick forebrain neurons. Cell Mol Neurobiol. 2001;21:437-51 pubmed
    ..4. These findings suggest a specific calcium-dependent regulatory pathway for modulating actomyosin during growth cone motility. ..
  5. request reprint
    Alexanian A, Arutyunian N. Reversal of drug resistance in sarcoma-45 by the new calmodulin antagonist--trihydrochloride of [1,2,5-trimethyl-4-phenyl-4-beta-[N-(beta-ethylamino)-N-4'-methoxybe nzy l]-ethylamino] piperidine (AR-2). Invest New Drugs. 1999;17:105-10 pubmed
    ..It is concluded that the effect of AR-2 can be explained by both hydrophobic and electrostatic interaction with a protein target (170 kDa P-glycoprotein) in resistant sarcoma-45 tumor cell's membrane. ..
  6. request reprint
    Alexanian A, Sieber Blum M. Differentiating adult hippocampal stem cells into neural crest derivatives. Neuroscience. 2003;118:1-5 pubmed
    ..The data suggest that neural crest cell-derived short-range cues that are recognized across species can instruct adult mHNSC to differentiate into neural crest phenotypes. ..
  7. request reprint
    Alexanian A, Crowe M, Kurpad S. Efficient differentiation and integration of lineage-restricted neural precursors in the traumatically injured adult cat spinal cord. J Neurosci Methods. 2006;150:41-6 pubmed
    ..We conclude that a mixture of more restricted neural precursors may be better suited than highly immature NSCs for neural replacement strategies after central nervous system (CNS) injuries. ..
  8. request reprint
    Alexanian A. Epigenetic modifiers promote efficient generation of neural-like cells from bone marrow-derived mesenchymal cells grown in neural environment. J Cell Biochem. 2007;100:362-71 pubmed
    ..We concluded that the manipulation strategy used in this study can be a useful method for efficient production of NSC-like cells from MSCs. ..
  9. Alexanian A, Maiman D, Kurpad S, Gennarelli T. In vitro and in vivo characterization of neurally modified mesenchymal stem cells induced by epigenetic modifiers and neural stem cell environment. Stem Cells Dev. 2008;17:1123-30 pubmed publisher
    ....
  10. Alexanian A, Svendsen C, Crowe M, Kurpad S. Transplantation of human glial-restricted neural precursors into injured spinal cord promotes functional and sensory recovery without causing allodynia. Cytotherapy. 2011;13:61-8 pubmed publisher
    ..Further studies will be conducted to investigate the ability of A2B5(+) NG2(+) cells to survive, differentiate and integrate in the injured spinal cord. ..

Locale

Detail Information

Publications17

  1. request reprint
    Macias M, Syring M, Pizzi M, Crowe M, Alexanian A, Kurpad S. Pain with no gain: allodynia following neural stem cell transplantation in spinal cord injury. Exp Neurol. 2006;201:335-48 pubmed
    ..Both allodynia- and CGRP/GAP43-positive afferent sprouting were less in the C17.2/GDNF group compared to the C17.2 group, suggesting a possible protective or analgesic effect of GDNF on post-injury neuropathic pain. ..
  2. request reprint
    Alexanian A, Bamburg J. Neuronal survival activity of s100betabeta is enhanced by calcineurin inhibitors and requires activation of NF-kappaB. FASEB J. 1999;13:1611-20 pubmed
    ..We conclude that S100betabeta enhances the survival of chick embryo forebrain neurons through the activation of NF-kappaB. ..
  3. Alexanian A, Fehlings M, Zhang Z, Maiman D. Transplanted neurally modified bone marrow-derived mesenchymal stem cells promote tissue protection and locomotor recovery in spinal cord injured rats. Neurorehabil Neural Repair. 2011;25:873-80 pubmed publisher
    ..Transplantation also reduced the volume of lesion cavity and white matter loss. This method of hMSC modification may provide an alternative source of autologous adult stem cells for CNS repair. ..
  4. request reprint
    Alexanian A, Bamburg J, Hidaka H, Mornet D. Calcium-dependent regulation of interactions of caldesmon with calcium-binding proteins found in growth cones of chick forebrain neurons. Cell Mol Neurobiol. 2001;21:437-51 pubmed
    ..4. These findings suggest a specific calcium-dependent regulatory pathway for modulating actomyosin during growth cone motility. ..
  5. request reprint
    Alexanian A, Arutyunian N. Reversal of drug resistance in sarcoma-45 by the new calmodulin antagonist--trihydrochloride of [1,2,5-trimethyl-4-phenyl-4-beta-[N-(beta-ethylamino)-N-4'-methoxybe nzy l]-ethylamino] piperidine (AR-2). Invest New Drugs. 1999;17:105-10 pubmed
    ..It is concluded that the effect of AR-2 can be explained by both hydrophobic and electrostatic interaction with a protein target (170 kDa P-glycoprotein) in resistant sarcoma-45 tumor cell's membrane. ..
  6. request reprint
    Alexanian A, Sieber Blum M. Differentiating adult hippocampal stem cells into neural crest derivatives. Neuroscience. 2003;118:1-5 pubmed
    ..The data suggest that neural crest cell-derived short-range cues that are recognized across species can instruct adult mHNSC to differentiate into neural crest phenotypes. ..
  7. request reprint
    Alexanian A, Crowe M, Kurpad S. Efficient differentiation and integration of lineage-restricted neural precursors in the traumatically injured adult cat spinal cord. J Neurosci Methods. 2006;150:41-6 pubmed
    ..We conclude that a mixture of more restricted neural precursors may be better suited than highly immature NSCs for neural replacement strategies after central nervous system (CNS) injuries. ..
  8. request reprint
    Alexanian A. Epigenetic modifiers promote efficient generation of neural-like cells from bone marrow-derived mesenchymal cells grown in neural environment. J Cell Biochem. 2007;100:362-71 pubmed
    ..We concluded that the manipulation strategy used in this study can be a useful method for efficient production of NSC-like cells from MSCs. ..
  9. Alexanian A, Maiman D, Kurpad S, Gennarelli T. In vitro and in vivo characterization of neurally modified mesenchymal stem cells induced by epigenetic modifiers and neural stem cell environment. Stem Cells Dev. 2008;17:1123-30 pubmed publisher
    ....
  10. Alexanian A, Svendsen C, Crowe M, Kurpad S. Transplantation of human glial-restricted neural precursors into injured spinal cord promotes functional and sensory recovery without causing allodynia. Cytotherapy. 2011;13:61-8 pubmed publisher
    ..Further studies will be conducted to investigate the ability of A2B5(+) NG2(+) cells to survive, differentiate and integrate in the injured spinal cord. ..
  11. request reprint
    Alexanian A, Nornes H. Proliferation and regeneration of retrogradely labeled adult rat corticospinal neurons in culture. Exp Neurol. 2001;170:277-82 pubmed
    ..The result shows that adult corticospinal tract neurons are capable of DNA synthesis, and our total cell counts with labeled cells counts further suggest that these cells undergo cell division. ..
  12. request reprint
    Alexanian A, Kurpad S. Quiescent neural cells regain multipotent stem cell characteristics influenced by adult neural stem cells in co-culture. Exp Neurol. 2005;191:193-7 pubmed
    ..We conclude that reactivation of quiescent neural cells can be initiated by NSC-associated short-range cues but not by cell fusion. ..
  13. request reprint
    Alexanian A. Neural stem cells induce bone-marrow-derived mesenchymal stem cells to generate neural stem-like cells via juxtacrine and paracrine interactions. Exp Cell Res. 2005;310:383-91 pubmed
    ..We conclude that juxtacrine interaction between NSCs and MSCs combined with soluble factors released from NSCs are important for generation of neural-like cells from bone-marrow-derived adherent MSCs. ..
  14. Alexanian A. An efficient method for generation of neural-like cells from adult human bone marrow-derived mesenchymal stem cells. Regen Med. 2010;5:891-900 pubmed publisher
    ..Human MSCs that are neurally modified with this methodology could be a useful source of cells for CNS repair and regeneration. ..
  15. Alexanian A, Kwok W, Pravdic D, Maiman D, Fehlings M. Survival of neurally induced mesenchymal cells may determine degree of motor recovery in injured spinal cord rats. Restor Neurol Neurosci. 2010;28:761-7 pubmed publisher
    ..Immunocytochemical studies revealed poor survival of NI-MSCs within the ISC as early as 3 weeks after transplantation. Thus, there is a correlation between the degree of surviving NI-MSCs and extent of functional recovery. ..
  16. Zhang Z, Maiman D, Kurpad S, Crowe M, Alexanian A. Feline bone marrow-derived mesenchymal stem cells express several pluripotent and neural markers and easily turn into neural-like cells by manipulation with chromatin modifying agents and neural inducing factors. Cell Reprogram. 2011;13:385-90 pubmed publisher
    ..We concluded that the expression of pluripotency- and neural-associated genes in unmodified fMSCs make them more pliable for reprogramming into a neural fate by manipulation with chromatin modifying agents and neural inducing factors. ..
  17. request reprint
    Alexanian A, Arutyunian N, Galoyan A. Antiarrhythmic action of the new calcium antagonist [1,2,5-trimethyl-4-phenyl-4-beta-(N-cyanoethyl-N-4'-methoxybenzyl) -ethylamino]piperidine dihydrochloride. Arzneimittelforschung. 1996;46:1110-3 pubmed
    ....