Research Topics
Genomes and Genes | Hai Long WangSummaryAffiliation: Mayo Clinic Country: USA Publications
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Publications
Control of cation permeation through the nicotinic receptor channelHai Long Wang
Receptor Biology Laboratory, Department of Physiology, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
PLoS Comput Biol 4:e41. 2008....- Single-channel current through nicotinic receptor produced by closure of binding site C-loopHai Long Wang
Receptor Biology Laboratory, Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
Biophys J 96:3582-90. 2009..This approach of applying Biology Boltzmann transport Monte Carlo simulation can be used to further investigate the binding to gating transduction mechanism and the structural bases for ion selection and translocation... 
Lysine scanning mutagenesis delineates structural model of the nicotinic receptor ligand binding domainSteven M Sine
Receptor Biology Laboratory, Department of Physiology and Biophysics, Mayo Foundation, Rochester, Minnesota 55905, USA
J Biol Chem 277:29210-23. 2002..The overall results suggest that lysine scanning can provide the basis for structural modeling of other members of the AChR superfamily as well as of other proteins with repeating structures delimiting a hydrophobic core...- Curariform antagonists bind in different orientations to acetylcholine-binding proteinFan Gao
Receptor Biology Laboratory, Department of Physiology and Biophysics, Mayo Clinic Department of Pharmacology, Mayo Graduate School, Rochester, Minnesota 55905, USA
J Biol Chem 278:23020-6. 2003..Thus structurally similar ligands can adopt distinct orientations at receptor binding sites, posing challenges for interpreting structure-activity relationships for many drugs... - Naturally occurring mutations at the acetylcholine receptor binding site independently alter ACh binding and channel gatingSteven M Sine
Receptor Biology Laboratory, Department of Physiology and Biophysics, and Mayo Foundation, Rochester, MN 55905, USA
J Gen Physiol 120:483-96. 2002..The overall results show that key residues at the ACh binding site differentially stabilize the agonist bound to closed, open and desensitized states, and provide a set point for gating of the channel... - On the origin of ion selectivity in the Cys-loop receptor familySteven M Sine
Receptor Biology Laboratory, Departments of Physiology and Biomedical Engineering and Neurology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
J Mol Neurosci 40:70-6. 2010.... - Mechanistic diversity underlying fast channel congenital myasthenic syndromesSteven M Sine
Receptor Biology Laboratory, Department of Physiology and Biophysics, Mayo Medical School, Rochester, Minnesota 55905, USA
Ann N Y Acad Sci 998:128-37. 2003..This review focuses on new mechanisms underlying the FCCMS... - Toward atomic-scale understanding of ligand recognition in the muscle nicotinic receptorSteven M Sine
Receptor Biology Laboratory, Department of Physiology and Biophysics, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
Curr Med Chem 11:559-67. 2004..The results have important implications for design of drugs targeting nicotinic receptors and members of the superfamily of pentameric ligand-gated ion channels... - Intramembrane proton binding site linked to activation of bacterial pentameric ion channelHai Long Wang
Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
J Biol Chem 287:6482-9. 2012..5 pH unit range. MD simulations show that mutations of Tyr-263 alter the hydrogen bonding capacity of His-235. The overall findings show that His-235 in the TM region of GLIC is a novel proton binding site linked to channel activation... - Impaired Ca2+-dependent activation of large-conductance Ca2+-activated K+ channels in the coronary artery smooth muscle cells of Zucker Diabetic Fatty ratsTong Lu
Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA
Biophys J 95:5165-77. 2008..These abnormalities in BK channel gating lead to an increase in the energy barrier for channel activation, and may contribute to the development of vascular dysfunction and complications in type 2 diabetes mellitus... - Recent structural and mechanistic insights into endplate acetylcholine receptorsSteven M Sine
Receptor Biology Laboratory, Departments of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Ann N Y Acad Sci 1132:53-60. 2008..Structural models of the human AChR enable precise mapping of disease-causing mutations, while studies of the speed with which single AChR channels open and close cast light on pathogenic mechanisms... - Detection and trapping of intermediate states priming nicotinic receptor channel openingNuriya Mukhtasimova
Receptor Biology Laboratory, Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
Nature 459:451-4. 2009..Thus, a change in binding-site conformation primes the AChR for channel opening in a process that enables selective activation by ACh while maximizing the speed and efficiency of the biological response... - Curariform antagonists bind in different orientations to the nicotinic receptor ligand binding domainHai-long Wang
Receptor Biology Laboratory, Department of Physiology and Biophysics, Mayo Clinic, Rochester, Minnesota 55905, USA
J Biol Chem 278:32284-91. 2003..The overall mutagenesis and computational results show that despite their similar structures, d-TC and metocurine bind in distinctly different orientations to the adult human AChR... - Ligand-induced conformational change in the alpha7 nicotinic receptor ligand binding domainRichard H Henchman
Howard Hughes Medical Institute, NSF Center for Theoretical Biophysics, Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, USA
Biophys J 88:2564-76. 2005.... - Alpha-conotoxins ImI and ImII target distinct regions of the human alpha7 nicotinic acetylcholine receptor and distinguish human nicotinic receptor subtypesMichael Ellison
Department of Biology and Psychiatry, University of Utah, Salt Lake City, Utah 84112, USA
Biochemistry 43:16019-26. 2004..Collectively, the data show that while alpha-ImI targets the classical competitive ligand binding site in a subtype selective manner, alpha-ImII is a probe of a novel inhibitory site in homomeric alpha7 nAChRs... - Coupling of agonist binding to channel gating in an ACh-binding protein linked to an ion channelCecilia Bouzat
Instituto de Investigaciones Bioquimicas, UNS-CONICET, Bahia Blanca 8000, Argentina
Nature 430:896-900. 2004..Structural modelling reveals a network of interacting loops between binding and pore domains that mediates this allosteric coupling process... - Asymmetric structural motions of the homomeric alpha7 nicotinic receptor ligand binding domain revealed by molecular dynamics simulationRichard H Henchman
Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, California 92093, USA
Biophys J 85:3007-18. 2003..Furthermore, the shape of the asymmetry with binding sites of differing affinity for acetylcholine, characteristic of other nicotinic receptors, may be a natural property of the relaxed, activatable state of alpha7... - The regulatory domains of CNA have different effects on the inhibition of CN activity by FK506 and CsAHai Long Wang
Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory, Beijing, PR China
IUBMB Life 59:388-93. 2007..The LCBD has no effect and the AID reduces the inhibition of CN by two complexes. In addition, LCBD and AID as autoinhibitors may inhibit enzyme activity via different sites... - Density functional theory studies on the electronic and vibrational spectra of octaethylporphyrin diacidZun Yun Li
Department of Chemical Physics, University of Science and Technology of China, Hefei, Anhui 230026, People s Republic of China
Spectrochim Acta A Mol Biomol Spectrosc 67:1382-91. 2007..The Raman and IR spectra of H4OEP2+ and the Raman spectrum of its N-deuterated analogue (D4OEP2+) were measured. The observed Raman and IR bands have been assigned based on the frequency calculations at the B3LYP/6-31G* level of theory... - The importance of Loop 7 for the activity of calcineurinPing Liu
Department of Biochemistry and Molecular Biology, Beijing Normal University, Beijing Key Laboratory, Beijing 100875, PR China
FEBS Lett 577:205-8. 2004..We also found that the effect of Loop 7 on enzyme activity was substrate dependent, and involved interactions between Loop 7 residues and the unresolved part of the CN crystal structure near the auto-inhibitory domain and catalytic site... - [Polymorphism analysis of 825C/T of the G-protein beta 3 subunit in high risk population of hypertension in the northeast China]Shu Ping Dai
Department of Medical Genetics, China Medical University, Shenyang 110001, China
Yi Chuan Xue Bao 29:294-8. 2002..Although GNB3 is not a susceptible gene of hypertension in the northeast Chinese, it still has some effects on regulation of the blood pressure in susceptible women...