E M Poeschla

Summary

Affiliation: Mayo Clinic
Country: USA

Publications

  1. pmc Unintegrated lentivirus DNA persistence and accessibility to expression in nondividing cells: analysis with class I integrase mutants
    Dyana T Saenz
    Molecular Medicine Program, Departments of Immunology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    J Virol 78:2906-20. 2004
  2. pmc LEDGF/p75 proteins with alternative chromatin tethers are functional HIV-1 cofactors
    Anne M Meehan
    Department of Molecular Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
    PLoS Pathog 5:e1000522. 2009
  3. pmc A cyclophilin homology domain-independent role for Nup358 in HIV-1 infection
    Anne M Meehan
    Department of Molecular Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, United States of America
    PLoS Pathog 10:e1003969. 2014
  4. pmc Productive replication and evolution of HIV-1 in ferret cells
    Hind J Fadel
    Department of Molecular Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
    J Virol 86:2312-22. 2012
  5. pmc Primate and feline lentiviruses in current intrinsic immunity research: the cat is back
    Eric M Poeschla
    Department of Molecular Medicine and Division of Infectious Diseases, Guggenheim 18, College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Vet Immunol Immunopathol 143:215-20. 2011
  6. pmc Integrase, LEDGF/p75 and HIV replication
    E M Poeschla
    Guggenheim 18, Mayo Clinic College of Medicine, 200 First Street SW, Rochester 55905, USA
    Cell Mol Life Sci 65:1403-24. 2008
  7. ncbi Non-primate lentiviral vectors
    Eric M Poeschla
    Molecular Medicine Program, Departments of Immunology and Medicine, Mayo Medical School, 200 First Street SW, Rochester, MN 55905, USA
    Curr Opin Mol Ther 5:529-40. 2003
  8. pmc LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexes
    Manuel Llano
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    J Virol 78:9524-37. 2004
  9. ncbi Durable, safe, multi-gene lentiviral vector expression in feline trabecular meshwork
    Pranay D Khare
    1Molecular Medicine Program Mayo Clinic College of Medicine, Rochester, Minnesota, USA
    Mol Ther 16:97-106. 2008
  10. ncbi Long-term, targeted genetic modification of the aqueous humor outflow tract coupled with noninvasive imaging of gene expression in vivo
    Nils Loewen
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Invest Ophthalmol Vis Sci 45:3091-8. 2004

Collaborators

Detail Information

Publications39

  1. pmc Unintegrated lentivirus DNA persistence and accessibility to expression in nondividing cells: analysis with class I integrase mutants
    Dyana T Saenz
    Molecular Medicine Program, Departments of Immunology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    J Virol 78:2906-20. 2004
    ....
  2. pmc LEDGF/p75 proteins with alternative chromatin tethers are functional HIV-1 cofactors
    Anne M Meehan
    Department of Molecular Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
    PLoS Pathog 5:e1000522. 2009
    ..NDE mutants were corroborative. Chromatin tethering per se is a basic HIV-1 requirement and this rather than engagement of particular chromatin ligands is important for the LEDGF/p75 cofactor mechanism...
  3. pmc A cyclophilin homology domain-independent role for Nup358 in HIV-1 infection
    Anne M Meehan
    Department of Molecular Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, United States of America
    PLoS Pathog 10:e1003969. 2014
    ..However, the results do not suggest direct roles for the Nup358 cyclophilin or SUMO E3 ligase domains in engaging the HIV-1 capsid prior to nuclear translocation...
  4. pmc Productive replication and evolution of HIV-1 in ferret cells
    Hind J Fadel
    Department of Molecular Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
    J Virol 86:2312-22. 2012
    ..We suggest that further characterization of HIV-1 adaptation in ferret cells and delineation of Mustelidae restriction factor repertoires are warranted, with a view to the potential for an HIV-1 animal model...
  5. pmc Primate and feline lentiviruses in current intrinsic immunity research: the cat is back
    Eric M Poeschla
    Department of Molecular Medicine and Division of Infectious Diseases, Guggenheim 18, College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Vet Immunol Immunopathol 143:215-20. 2011
    ..These can be overcome by inserting FIV vif or even SIVmac vif into HIV-1. The domestic cat and its lentivirus are positioned to offer strategic research opportunities as the field moves forward...
  6. pmc Integrase, LEDGF/p75 and HIV replication
    E M Poeschla
    Guggenheim 18, Mayo Clinic College of Medicine, 200 First Street SW, Rochester 55905, USA
    Cell Mol Life Sci 65:1403-24. 2008
    ..Current goals are to establish the underlying mechanisms and to determine whether this knowledge can be exploited for antiviral therapy or for targeting lentiviral vector integration in human gene therapy...
  7. ncbi Non-primate lentiviral vectors
    Eric M Poeschla
    Molecular Medicine Program, Departments of Immunology and Medicine, Mayo Medical School, 200 First Street SW, Rochester, MN 55905, USA
    Curr Opin Mol Ther 5:529-40. 2003
    ..This review summarizes recent results in this growing field and discusses elements of an agenda for furthering application of these vectors to human gene therapy...
  8. pmc LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexes
    Manuel Llano
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    J Virol 78:9524-37. 2004
    ..While lentiviral PIC nuclear import is unaffected by LEDGF/p75 knockdown, this protein is a component of functional lentiviral PICs. A role in HIV-1 integration site distribution merits investigation...
  9. ncbi Durable, safe, multi-gene lentiviral vector expression in feline trabecular meshwork
    Pranay D Khare
    1Molecular Medicine Program Mayo Clinic College of Medicine, Rochester, Minnesota, USA
    Mol Ther 16:97-106. 2008
    ..These results demonstrate a safe, long-term single and dual gene expression in TM and establish an experimental system for testing candidate therapeutic transgenes for POAG...
  10. ncbi Long-term, targeted genetic modification of the aqueous humor outflow tract coupled with noninvasive imaging of gene expression in vivo
    Nils Loewen
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Invest Ophthalmol Vis Sci 45:3091-8. 2004
    ....
  11. ncbi Preservation of aqueous outflow facility after second-generation FIV vector-mediated expression of marker genes in anterior segments of human eyes
    Nils Loewen
    Molecular Medicine Program, Mayo Clinic, Rochester, Minnesota 55905, USA
    Invest Ophthalmol Vis Sci 43:3686-90. 2002
    ....
  12. ncbi Perfusion of his-tagged eukaryotic myocilin increases outflow resistance in human anterior segments in the presence of aqueous humor
    Michael P Fautsch
    Department of Ophthalmology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Invest Ophthalmol Vis Sci 47:213-21. 2006
    ..The present study was performed to determine whether full-length myocilin purified from a human trabecular meshwork cell expression system alters outflow resistance after infusion into human anterior segments...
  13. pmc Human gene therapy vectors derived from feline lentiviruses
    Román A Barraza
    Molecular Medicine Program, Guggenheim 18, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, United States
    Vet Immunol Immunopathol 123:23-31. 2008
    ..This article reviews significant progress in the development and refinement of FIV vector systems...
  14. ncbi An essential role for LEDGF/p75 in HIV integration
    Manuel Llano
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Science 314:461-4. 2006
    ..Perturbing the p75-integrase interaction may have therapeutic potential...
  15. pmc Characterization of monoclonal antibodies against the glaucoma-associated protein myocilin
    Mohamed Karim Ezzat
    Department of Ophthalmology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Exp Eye Res 87:376-84. 2008
    ..Myocilin was also detected by immunohistochemistry in trabecular meshwork, ciliary body, iris, cornea, sclera, choroid, and retinal pigment epithelial cells...
  16. pmc Prolonged transgene expression with lentiviral vectors in the aqueous humor outflow pathway of nonhuman primates
    Román A Barraza
    Department of Molecular Medicine, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Hum Gene Ther 20:191-200. 2009
    ..The results demonstrate the first lentiviral vector transduction of the nonhuman primate aqueous humor outflow pathway and support application of the system to human glaucoma gene therapy...
  17. doi Chromatin tethering and retroviral integration: recent discoveries and parallels with DNA viruses
    Anne M Meehan
    Department of Molecular Medicine and Division of Infectious Diseases, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Biochim Biophys Acta 1799:182-91. 2010
    ..Here we review the evidence supporting the LEDGF/p75 tethering model and consider parallels with these other viruses...
  18. pmc Prostaglandin pathway gene therapy for sustained reduction of intraocular pressure
    Román A Barraza
    Department of Molecular Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
    Mol Ther 18:491-501. 2010
    ..This study provides the first gene therapy for correcting the main cause of glaucoma...
  19. ncbi Approach to salvage antiretroviral therapy in heavily antiretroviral-experienced HIV-positive adults
    Zelalem Temesgen
    Division of Infectious Diseases, Mayo Clinic, Rochester, Minnesota 55905, USA
    Lancet Infect Dis 6:496-507. 2006
    ..Additionally, increased mortality has been associated with multidrug-resistant HIV. We review data relevant to management of such patients and offer a systematic approach to constructing a salvage antiretroviral regimen...
  20. ncbi Genetic modification of human trabecular meshwork with lentiviral vectors
    N Loewen
    Molecular Medicine Program, Mayo Clinic, Rochester, MN 55905, USA
    Hum Gene Ther 12:2109-19. 2001
    ..In addition, controlled comparisons in this study establish that feline and human immunodeficiency virus vectors are equivalently efficacious in delivering genes to this terminally differentiated human tissue...
  21. ncbi Lentiviral vectors
    Nils Loewen
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Adv Biochem Eng Biotechnol 99:169-91. 2005
    ..Innate cellular defenses against retroviruses and their implications for human gene therapy with different lentiviral vectors are also addressed...
  22. pmc Productive replication of Vif-chimeric HIV-1 in feline cells
    Melissa A Stern
    Guggenheim 1811A, College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    J Virol 84:7378-95. 2010
    ..The results raise the possibility that the domestic cat could yield an animal model of HIV-1 infection...
  23. ncbi FIV Vectors
    Nils Loewen
    Molecular Medicine Program, Mayo Clinic, Rochester, MN, USA
    Methods Mol Biol 229:251-71. 2003
  24. ncbi Lens epithelium-derived growth factor/p75 prevents proteasomal degradation of HIV-1 integrase
    Manuel Llano
    Molecular Medicine Program and Department of Immunology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    J Biol Chem 279:55570-7. 2004
    ..Protection requires only interaction with LEDGF/p75, and it is independent of the subcellular localization of the IN-LEDGF complex...
  25. ncbi The critical role of proximal gag sequences in feline immunodeficiency virus genome encapsidation
    Iris Kemler
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Virology 327:111-20. 2004
    ..Focusing further on this proximal sequence, we found that a deletion of only 13 nt at the 5' end of gag impaired encapsidation of subgenomic vector and proviral RNAs...
  26. ncbi Comparison of wild-type and class I integrase mutant-FIV vectors in retina demonstrates sustained expression of integrated transgenes in retinal pigment epithelium
    Nils Loewen
    Molecular Medicine Program, Mayo Clinic, Rochester, MN 55905, USA
    J Gene Med 5:1009-17. 2003
    ..Class I integrase mutants have not been described for feline immunodeficiency virus (FIV) integrase, or tested in the eye for any lentiviral vector...
  27. ncbi FIV: from lentivirus to lentivector
    Dyana T Saenz
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Gene Med 6:S95-104. 2004
    ..This review discusses virological aspects of lentiviral vector development, as well as some recent controversies and applications...
  28. ncbi Identification and characterization of the chromatin-binding domains of the HIV-1 integrase interactor LEDGF/p75
    Manuel Llano
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    J Mol Biol 360:760-73. 2006
    ..The results establish a molecular mechanism for LEDGF/p75-mediated tethering of HIV-1 integrase to chromatin...
  29. pmc Mapping the encapsidation determinants of feline immunodeficiency virus
    Iris Kemler
    Molecular Medicine Program, Departments of Immunology and Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Virol 76:11889-903. 2002
    ..The results identify a mechanism whereby FIV can encapsidate its genomic mRNA in preference to subgenomic mRNAs...
  30. pmc The HIV-1 central polypurine tract functions as a second line of defense against APOBEC3G/F
    Chunling Hu
    Department of Molecular Medicine, Mayo Clinic, Rochester, MN 55905, USA
    J Virol 84:11981-93. 2010
    ..The salient functional feature is central plus-strand initiation, which functions as a second line of defense against single-stranded DNA editing by A3 proteins that survive producer cell degradation by Vif...
  31. pmc Restriction of feline immunodeficiency virus by Ref1, Lv1, and primate TRIM5alpha proteins
    Dyana T Saenz
    Molecular Medicine Program, Guggenheim 18, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    J Virol 79:15175-88. 2005
    ..Arsenic trioxide had complex and TRIM5alpha-independent enhancing effects on lentiviral but not NB-MLV infection. Implications for human gene therapy are discussed...
  32. ncbi Long-term retinal transgene expression with FIV versus adenoviral vectors
    Nils Loewen
    Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN, USA
    Mol Vis 10:272-80. 2004
    ..We compared time course, location, and extent of transgene expression from replication-deficient feline immunodeficiency virus (FIV) vectors and Ad vectors in neonatal rat retina...
  33. pmc Intensive RNAi with lentiviral vectors in mammalian cells
    Tanner Miest
    Department of Molecular Medicine, Mayo Clinic College of Medicine, Guggenheim 1811A, 200 First Street SW, Rochester, MN 55905, USA
    Methods 47:298-303. 2009
    ..Critical controls are reviewed, including rescue of the pre-knockdown phenotype by re-expression of the targeted gene. The time from thinking about a potential knockdown target to analysis of phenotypes can be as short as a few weeks...
  34. pmc LEDGF dominant interference proteins demonstrate prenuclear exposure of HIV-1 integrase and synergize with LEDGF depletion to destroy viral infectivity
    Anne M Meehan
    Department of Molecular Medicine, College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    J Virol 85:3570-83. 2011
    ..LEDGF dominant interference and depletion impair HIV-1 integration at distinct postentry stages. GFP-IBD may trigger premature or improper integrase oligomerization...
  35. pmc Live-cell coimaging of the genomic RNAs and Gag proteins of two lentiviruses
    Iris Kemler
    Department of Molecular Medicine, Guggenheim 18, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    J Virol 84:6352-66. 2010
    ..Based on the FIV results, we hypothesize that the Gag-genome association may initiate at the nuclear envelope...
  36. ncbi An outbreak of neurological autoimmunity with polyradiculoneuropathy in workers exposed to aerosolised porcine neural tissue: a descriptive study
    Daniel H LaChance
    Department of Neurology, Mayo Clinic, Rochester, MN, USA Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
    Lancet Neurol 9:55-66. 2010
    ..We aimed to describe the pathogenic and immunological characteristics of this illness...
  37. pmc Blockade of human immunodeficiency virus type 1 expression by caveolin-1
    Manuel Llano
    Molecular Medicine Program and Departments of Immunology, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Virol 76:9152-64. 2002
    ..These data show that coexpression of caveolins can markedly inhibit expression of HIV proviral DNA and establish that the inhibition is mediated by the hydrophobic, membrane-associated domain...
  38. pmc Directed evolution of retrovirus envelope protein cytoplasmic tails guided by functional incorporation into lentivirus particles
    Christoph A Merten
    Division of Medical Biotechnology, Paul Ehrlich Institut, Paul Ehrlich Str 51 59, 63225 Langen, Germany
    J Virol 79:834-40. 2005
    ..Interestingly, in some of the selected variants, the R-peptide cleavage site had shifted closer to the C terminus. These data demonstrate a valuable approach for the engineering of chimeric viruses and vector particles...
  39. ncbi Codon optimization of the HIV-1 vpu and vif genes stabilizes their mRNA and allows for highly efficient Rev-independent expression
    Kim Lien Nguyen
    Viral Biochemistry Section, Laboratory of Molecular Microbiology, National Institutes of Allergy Diseases, Bethesda, MD 20892, USA
    Virology 319:163-75. 2004
    ....