Research Topics
| E M PoeschlaSummaryAffiliation: Mayo Clinic Country: USA Publications
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Detail Information
Publications
Unintegrated lentivirus DNA persistence and accessibility to expression in nondividing cells: analysis with class I integrase mutantsDyana T Saenz
Molecular Medicine Program, Departments of Immunology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
J Virol 78:2906-20. 2004....- Productive replication and evolution of HIV-1 in ferret cellsHind J Fadel
Department of Molecular Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
J Virol 86:2312-22. 2012..We suggest that further characterization of HIV-1 adaptation in ferret cells and delineation of Mustelidae restriction factor repertoires are warranted, with a view to the potential for an HIV-1 animal model... 
Non-primate lentiviral vectorsEric M Poeschla
Molecular Medicine Program, Departments of Immunology and Medicine, Mayo Medical School, 200 First Street SW, Rochester, MN 55905, USA
Curr Opin Mol Ther 5:529-40. 2003..This review summarizes recent results in this growing field and discusses elements of an agenda for furthering application of these vectors to human gene therapy...- Primate and feline lentiviruses in current intrinsic immunity research: the cat is backEric M Poeschla
Department of Molecular Medicine and Division of Infectious Diseases, Guggenheim 18, College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
Vet Immunol Immunopathol 143:215-20. 2011..These can be overcome by inserting FIV vif or even SIVmac vif into HIV-1. The domestic cat and its lentivirus are positioned to offer strategic research opportunities as the field moves forward... 
Integrase, LEDGF/p75 and HIV replicationE M Poeschla
Guggenheim 18, Mayo Clinic College of Medicine, 200 First Street SW, Rochester 55905, USA
Cell Mol Life Sci 65:1403-24. 2008..Current goals are to establish the underlying mechanisms and to determine whether this knowledge can be exploited for antiviral therapy or for targeting lentiviral vector integration in human gene therapy...- LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexesManuel Llano
Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
J Virol 78:9524-37. 2004..While lentiviral PIC nuclear import is unaffected by LEDGF/p75 knockdown, this protein is a component of functional lentiviral PICs. A role in HIV-1 integration site distribution merits investigation... - Preservation of aqueous outflow facility after second-generation FIV vector-mediated expression of marker genes in anterior segments of human eyesNils Loewen
Molecular Medicine Program, Mayo Clinic, Rochester, Minnesota 55905, USA
Invest Ophthalmol Vis Sci 43:3686-90. 2002..Transduction and expression of these marker genes results in small and transient changes in outflow facility, suggesting suitability of this class of vectors for glaucoma gene therapy... - Durable, safe, multi-gene lentiviral vector expression in feline trabecular meshworkPranay D Khare
1Molecular Medicine Program Mayo Clinic College of Medicine, Rochester, Minnesota, USA
Mol Ther 16:97-106. 2008..These results demonstrate a safe, long-term single and dual gene expression in TM and establish an experimental system for testing candidate therapeutic transgenes for POAG... - Long-term, targeted genetic modification of the aqueous humor outflow tract coupled with noninvasive imaging of gene expression in vivoNils Loewen
Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Invest Ophthalmol Vis Sci 45:3091-8. 2004..These studies provide a basis for developing realistic disease models and administering glaucoma gene therapy... - Approach to salvage antiretroviral therapy in heavily antiretroviral-experienced HIV-positive adultsZelalem Temesgen
Division of Infectious Diseases, Mayo Clinic, Rochester, Minnesota 55905, USA
Lancet Infect Dis 6:496-507. 2006..Additionally, increased mortality has been associated with multidrug-resistant HIV. We review data relevant to management of such patients and offer a systematic approach to constructing a salvage antiretroviral regimen... - An essential role for LEDGF/p75 in HIV integrationManuel Llano
Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Science 314:461-4. 2006..Perturbing the p75-integrase interaction may have therapeutic potential... - Human gene therapy vectors derived from feline lentivirusesRomán A Barraza
Molecular Medicine Program, Guggenheim 18, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, United States
Vet Immunol Immunopathol 123:23-31. 2008..This article reviews significant progress in the development and refinement of FIV vector systems... - Characterization of monoclonal antibodies against the glaucoma-associated protein myocilinMohamed Karim Ezzat
Department of Ophthalmology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
Exp Eye Res 87:376-84. 2008..Myocilin was also detected by immunohistochemistry in trabecular meshwork, ciliary body, iris, cornea, sclera, choroid, and retinal pigment epithelial cells... - Prolonged transgene expression with lentiviral vectors in the aqueous humor outflow pathway of nonhuman primatesRomán A Barraza
Department of Molecular Medicine, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
Hum Gene Ther 20:191-200. 2009..The results demonstrate the first lentiviral vector transduction of the nonhuman primate aqueous humor outflow pathway and support application of the system to human glaucoma gene therapy... - Chromatin tethering and retroviral integration: recent discoveries and parallels with DNA virusesAnne M Meehan
Department of Molecular Medicine and Division of Infectious Diseases, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Biochim Biophys Acta 1799:182-91. 2010..Here we review the evidence supporting the LEDGF/p75 tethering model and consider parallels with these other viruses... - Prostaglandin pathway gene therapy for sustained reduction of intraocular pressureRomán A Barraza
Department of Molecular Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
Mol Ther 18:491-501. 2010..This study provides the first gene therapy for correcting the main cause of glaucoma... - Perfusion of his-tagged eukaryotic myocilin increases outflow resistance in human anterior segments in the presence of aqueous humorMichael P Fautsch
Department of Ophthalmology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Invest Ophthalmol Vis Sci 47:213-21. 2006..The present study was performed to determine whether full-length myocilin purified from a human trabecular meshwork cell expression system alters outflow resistance after infusion into human anterior segments... - Genetic modification of human trabecular meshwork with lentiviral vectorsN Loewen
Molecular Medicine Program, Mayo Clinic, Rochester, MN 55905, USA
Hum Gene Ther 12:2109-19. 2001..In addition, controlled comparisons in this study establish that feline and human immunodeficiency virus vectors are equivalently efficacious in delivering genes to this terminally differentiated human tissue... - Lentiviral vectorsNils Loewen
Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Adv Biochem Eng Biotechnol 99:169-91. 2005..Innate cellular defenses against retroviruses and their implications for human gene therapy with different lentiviral vectors are also addressed... - Productive replication of Vif-chimeric HIV-1 in feline cellsMelissa A Stern
Guggenheim 1811A, College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
J Virol 84:7378-95. 2010..The results raise the possibility that the domestic cat could yield an animal model of HIV-1 infection... - FIV VectorsNils Loewen
Molecular Medicine Program, Mayo Clinic, Rochester, MN, USA
Methods Mol Biol 229:251-71. 2003 - LEDGF/p75 proteins with alternative chromatin tethers are functional HIV-1 cofactorsAnne M Meehan
Department of Molecular Medicine, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
PLoS Pathog 5:e1000522. 2009..NDE mutants were corroborative. Chromatin tethering per se is a basic HIV-1 requirement and this rather than engagement of particular chromatin ligands is important for the LEDGF/p75 cofactor mechanism... - The critical role of proximal gag sequences in feline immunodeficiency virus genome encapsidationIris Kemler
Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Virology 327:111-20. 2004..Focusing further on this proximal sequence, we found that a deletion of only 13 nt at the 5' end of gag impaired encapsidation of subgenomic vector and proviral RNAs... - Lens epithelium-derived growth factor/p75 prevents proteasomal degradation of HIV-1 integraseManuel Llano
Molecular Medicine Program and Department of Immunology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
J Biol Chem 279:55570-7. 2004..Protection requires only interaction with LEDGF/p75, and it is independent of the subcellular localization of the IN-LEDGF complex... - FIV: from lentivirus to lentivectorDyana T Saenz
Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
J Gene Med 6:S95-104. 2004..This review discusses virological aspects of lentiviral vector development, as well as some recent controversies and applications... - Comparison of wild-type and class I integrase mutant-FIV vectors in retina demonstrates sustained expression of integrated transgenes in retinal pigment epitheliumNils Loewen
Molecular Medicine Program, Mayo Clinic, Rochester, MN 55905, USA
J Gene Med 5:1009-17. 2003..Transgene expression is mostly restricted to RPE after post-natal day 7 in rats, suggesting that FIV vectors could be used to target RPE for gene therapy... - Long-term retinal transgene expression with FIV versus adenoviral vectorsNils Loewen
Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN, USA
Mol Vis 10:272-80. 2004..The persistent expression at 16 months of follow-up suggests that these lentiviral vectors are useful for gene therapy of chronic retinal diseases... - The HIV-1 central polypurine tract functions as a second line of defense against APOBEC3G/FChunling Hu
Department of Molecular Medicine, Mayo Clinic, Rochester, MN 55905, USA
J Virol 84:11981-93. 2010..The salient functional feature is central plus-strand initiation, which functions as a second line of defense against single-stranded DNA editing by A3 proteins that survive producer cell degradation by Vif... - Restriction of feline immunodeficiency virus by Ref1, Lv1, and primate TRIM5alpha proteinsDyana T Saenz
Molecular Medicine Program, Guggenheim 18, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
J Virol 79:15175-88. 2005..Arsenic trioxide had complex and TRIM5alpha-independent enhancing effects on lentiviral but not NB-MLV infection. Implications for human gene therapy are discussed... - Mapping the encapsidation determinants of feline immunodeficiency virusIris Kemler
Molecular Medicine Program, Departments of Immunology and Medicine, Mayo Clinic, Rochester, Minnesota 55905, USA
J Virol 76:11889-903. 2002..The results identify a mechanism whereby FIV can encapsidate its genomic mRNA in preference to subgenomic mRNAs... - Identification and characterization of the chromatin-binding domains of the HIV-1 integrase interactor LEDGF/p75Manuel Llano
Molecular Medicine Program, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
J Mol Biol 360:760-73. 2006..The results establish a molecular mechanism for LEDGF/p75-mediated tethering of HIV-1 integrase to chromatin... - Intensive RNAi with lentiviral vectors in mammalian cellsTanner Miest
Department of Molecular Medicine, Mayo Clinic College of Medicine, Guggenheim 1811A, 200 First Street SW, Rochester, MN 55905, USA
Methods 47:298-303. 2009..Critical controls are reviewed, including rescue of the pre-knockdown phenotype by re-expression of the targeted gene. The time from thinking about a potential knockdown target to analysis of phenotypes can be as short as a few weeks... - LEDGF dominant interference proteins demonstrate prenuclear exposure of HIV-1 integrase and synergize with LEDGF depletion to destroy viral infectivityAnne M Meehan
Department of Molecular Medicine, College of Medicine, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
J Virol 85:3570-83. 2011..LEDGF dominant interference and depletion impair HIV-1 integration at distinct postentry stages. GFP-IBD may trigger premature or improper integrase oligomerization... - Live-cell coimaging of the genomic RNAs and Gag proteins of two lentivirusesIris Kemler
Department of Molecular Medicine, Guggenheim 18, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
J Virol 84:6352-66. 2010..Based on the FIV results, we hypothesize that the Gag-genome association may initiate at the nuclear envelope... - Blockade of human immunodeficiency virus type 1 expression by caveolin-1Manuel Llano
Molecular Medicine Program and Departments of Immunology, Mayo Clinic, Rochester, Minnesota 55905, USA
J Virol 76:9152-64. 2002..These data show that coexpression of caveolins can markedly inhibit expression of HIV proviral DNA and establish that the inhibition is mediated by the hydrophobic, membrane-associated domain... - An outbreak of neurological autoimmunity with polyradiculoneuropathy in workers exposed to aerosolised porcine neural tissue: a descriptive studyDaniel H LaChance
Department of Neurology, Mayo Clinic, Rochester, MN, USA Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA
Lancet Neurol 9:55-66. 2010..We aimed to describe the pathogenic and immunological characteristics of this illness... - Directed evolution of retrovirus envelope protein cytoplasmic tails guided by functional incorporation into lentivirus particlesChristoph A Merten
Division of Medical Biotechnology, Paul Ehrlich Institut, Paul Ehrlich Str 51 59, 63225 Langen, Germany
J Virol 79:834-40. 2005..Interestingly, in some of the selected variants, the R-peptide cleavage site had shifted closer to the C terminus. These data demonstrate a valuable approach for the engineering of chimeric viruses and vector particles... - Codon optimization of the HIV-1 vpu and vif genes stabilizes their mRNA and allows for highly efficient Rev-independent expressionKim Lien Nguyen
Viral Biochemistry Section, Laboratory of Molecular Microbiology, National Institutes of Allergy Diseases, Bethesda, MD 20892, USA
Virology 319:163-75. 2004....