Genomes and Genes
Douglas A Plager
Affiliation: Mayo Clinic
- A novel and highly divergent homolog of human eosinophil granule major basic proteinD A Plager
Departments of Immunology, Internal Medicine, and Biochemistry and Molecular Biology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA
J Biol Chem 274:14464-73. 1999..Overall, this novel homolog's unique physical properties indicated that the high net positive charge of hMBP is important but not essential for biological activity...
- Gene transcription changes in asthmatic chronic rhinosinusitis with nasal polyps and comparison to those in atopic dermatitisDouglas A Plager
Allergic Diseases Research Laboratory, Mayo Clinic, Rochester, Minnesota, United States of America
PLoS ONE 5:e11450. 2010....
- Eosinophil ribonucleases and their cutaneous lesion-forming activityDouglas A Plager
Department of Dermatology, Mayo Clinic, Rochester, MN 55905, USA
J Immunol 183:4013-20. 2009..In conclusion, ECP and EDN disrupt skin integrity and cause inflammation. Their presence in ulcerative skin lesions may explain certain findings in human eosinophil-associated diseases...
- Pimecrolimus reduces eosinophil activation associated with calcium mobilizationDouglas A Plager
Allergic Diseases Research Laboratory, Department of Dermatology, Mayo Clinic, Guggenheim 4 94, 200 First Street Southwest, Rochester, MN 55905, USA
Int Arch Allergy Immunol 149:119-26. 2009..However, its effects on eosinophils, a cell type implicated in allergic disease pathology, are unknown. Therefore, we examined the effects of pimecrolimus on eosinophil superoxide anion production, degranulation and survival...
- Early cutaneous gene transcription changes in adult atopic dermatitis and potential clinical implicationsDouglas A Plager
Department of Dermatology, Mayo Clinic and Foundation, Rochester, MN 55905, USA
Exp Dermatol 16:28-36. 2007..Identification of these genes and pathways will direct future research towards controlling AD...
- Major basic protein homolog (MBP2): a specific human eosinophil markerDouglas A Plager
Allergic Diseases Research Laboratory, Mayo Clinic and Foundation, Rochester, MN 55905, USA
J Immunol 177:7340-5. 2006..These results indicate that MBP2 is present only in eosinophils and that it may be a useful biomarker for eosinophil-associated diseases...
- A novel human homolog of eosinophil major basic proteinD A Plager
Department of Immunology, Mayo Clinic and Mayo Foundation, Rochester, MN 55905, USA
Immunol Rev 179:192-202. 2001....
- Comparative structure, proximal promoter elements, and chromosome location of the human eosinophil major basic protein genesD A Plager
Department of Immunology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota 55905, USA
Genomics 71:271-81. 2001..Furthermore, the identified conserved and distinct proximal promoter elements likely contribute to the eosinophil-restricted and relatively reduced transcription of the human MBPH gene...
- Differential extraction of eosinophil granule proteinsLyo E Ohnuki
Department of Dermatology, University of Utah, Salt Lake City, 84132, USA
J Immunol Methods 307:54-61. 2005..These results indicate that repetitive extraction of eosinophil granules yields an increased amount of all granule proteins, and that MBP2 can now be recovered in good quantities and in a relatively pure form...
- Interactions of eosinophil granule proteins with skin: limits of detection, persistence, and vasopermeabilizationMark D P Davis
Department of Dermatology, Mayo Clinic and Foundation, Rochester, Minn, USA
J Allergy Clin Immunol 112:988-94. 2003....
- Identification of basophils by a mAb directed against pro-major basic protein 1Douglas A Plager
Department of Dermatology, Mayo Clinic Rochester, MN, and Department of Internal Medicine, Virginia Commonwealth University, USA
J Allergy Clin Immunol 117:626-34. 2006..A mAb (J175-7D4) generated against the recombinant pro-form of human eosinophil granule major basic protein 1 (rproMBP1) appeared to stain only basophils in tissue specimens...
- Extensive fractionation and identification of proteins within nasal lavage fluids from allergic rhinitis and asthmatic chronic rhinosinusitis patientsLinda M Benson
Mayo Proteomics Research Center, Mayo Clinic, Rochester, MN, USA
J Sep Sci 32:44-56. 2009..This is the first extensive list of NLF proteins generated from CRS patients with coexisting asthma...
- Gene transcription abnormalities in canine atopic dermatitis and related human eosinophilic allergic diseasesDouglas A Plager
Allergic Diseases Research Laboratory, Mayo Clinic, Rochester, MN 55905, USA
Vet Immunol Immunopathol 149:136-42. 2012..Overall, numerous abnormally transcribed genes were observed only in canine AD; however, many others are common to related human eosinophilic allergic diseases and represent therapeutic targets testable in dogs with AD...
- Global Gene Expression in Chronic RhinosinusitisDOUGLAS PLAGER; Fiscal Year: 2005..These new molecular approaches should shed light on the mechanisms of CRS and open novel avenues for diagnosis and treatment of this difficult disease. ..