Maoqing Dong

Summary

Affiliation: Mayo Clinic
Country: USA

Publications

  1. pmc Site of action of a pentapeptide agonist at the glucagon-like peptide-1 receptor. Insight into a small molecule agonist-binding pocket
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 13400 East Shea Boulevard, Scottsdale, AZ 85259, USA
    Bioorg Med Chem Lett 22:638-41. 2012
  2. ncbi request reprint Importance of the amino terminus in secretin family G protein-coupled receptors. Intrinsic photoaffinity labeling establishes initial docking constraints for the calcitonin receptor
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    J Biol Chem 279:1167-75. 2004
  3. doi request reprint Exploration of the endogenous agonist mechanism for activation of secretin and VPAC1 receptors using synthetic glycosylated peptides
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 13400 East Shea Boulevard, Scottsdale, AZ 85259, USA
    J Mol Neurosci 36:254-9. 2008
  4. pmc Insights into the structural basis of endogenous agonist activation of family B G protein-coupled receptors
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    Mol Endocrinol 22:1489-99. 2008
  5. ncbi request reprint Molecular approximation between residue 10 of secretin and its receptor demonstrated by photoaffinity labeling
    Maoqing Dong
    Mayo Clinic Scottsdale, 13400 East Shea Blvd, Scottsdale, AZ 85259, USA
    Ann N Y Acad Sci 1070:243-7. 2006
  6. pmc Juxtamembranous region of the amino terminus of the family B G protein-coupled calcitonin receptor plays a critical role in small-molecule agonist action
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    J Biol Chem 284:21839-47. 2009
  7. ncbi request reprint Molecular approximation between a residue in the amino-terminal region of calcitonin and the third extracellular loop of the class B G protein-coupled calcitonin receptor
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    J Biol Chem 279:31177-82. 2004
  8. pmc Mapping spatial approximations between the amino terminus of secretin and each of the extracellular loops of its receptor using cysteine trapping
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    FASEB J 26:5092-105. 2012
  9. pmc Functional importance of a structurally distinct homodimeric complex of the family B G protein-coupled secretin receptor
    Fan Gao
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, AZ 85259, USA
    Mol Pharmacol 76:264-74. 2009
  10. doi request reprint Spatial approximation between secretin residue five and the third extracellular loop of its receptor provides new insight into the molecular basis of natural agonist binding
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 13400 East Shea Blvd, Scottsdale, AZ 85259, USA
    Mol Pharmacol 74:413-22. 2008

Collaborators

  • L J Miller
  • Patrick M Sexton
  • Ruben Abagyan
  • Andrew J Bordner
  • Arthur Christopoulos
  • Denise Wootten
  • Sebastian G B Furness
  • Xi Qin Ding
  • J C Reubi
  • Kaleeckal G Harikumar
  • Delia I Pinon
  • Bunzo Matsuura
  • Quan Chen
  • Polo C H Lam
  • Morikazu Onji
  • Jerez A Te
  • Alicja M Ball
  • Gene L Garcia
  • Erin E Cawston
  • Fan Gao
  • Hitoo Tokunaga
  • Gregory M Hayes
  • Eiji Takeshita
  • Vi Pham
  • Cayle S Lisenbee
  • Terry P Lybrand
  • Yan W Asmann
  • Sonnet J H Arlander
  • Mengwei Zang
  • Wei Qun Ding
  • Mary Lou Augustine
  • Andrew Orry
  • Eyup Akgün
  • Philip S Portoghese
  • Cassandra Koole
  • Bim Graham
  • Yoichi Hiasa
  • Shinya Furukawa
  • Teruhisa Ueda
  • Patricia E Carrigan
  • Zhijie Cheng
  • Shruthi Naik
  • Hidetaka Matsui
  • Hooi Ling Ng
  • John D Wade
  • Jeremy Clain
  • Bernard Coulie
  • Craig J Morton
  • Michael W Parker
  • Elizabeth M Hadac
  • Zhijun Li
  • Dora Ninova
  • Mark D Stegall
  • Eileen L Holicky

Detail Information

Publications59

  1. pmc Site of action of a pentapeptide agonist at the glucagon-like peptide-1 receptor. Insight into a small molecule agonist-binding pocket
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 13400 East Shea Boulevard, Scottsdale, AZ 85259, USA
    Bioorg Med Chem Lett 22:638-41. 2012
    ....
  2. ncbi request reprint Importance of the amino terminus in secretin family G protein-coupled receptors. Intrinsic photoaffinity labeling establishes initial docking constraints for the calcitonin receptor
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    J Biol Chem 279:1167-75. 2004
    ..These data are consistent with affinity labeling of other members of the class B G protein-coupled receptors using analogous probes and may suggest a common ligand binding mechanism for this family...
  3. doi request reprint Exploration of the endogenous agonist mechanism for activation of secretin and VPAC1 receptors using synthetic glycosylated peptides
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 13400 East Shea Boulevard, Scottsdale, AZ 85259, USA
    J Mol Neurosci 36:254-9. 2008
    ..These data provide further evidence for this proposed mechanism for the activation of this family of receptors...
  4. pmc Insights into the structural basis of endogenous agonist activation of family B G protein-coupled receptors
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    Mol Endocrinol 22:1489-99. 2008
    ..This directly supports the relevance of the endogenous agonist concept to the GLP1 receptor and provides new insights into the rational development and refinement of new types of drugs activating this important receptor...
  5. ncbi request reprint Molecular approximation between residue 10 of secretin and its receptor demonstrated by photoaffinity labeling
    Maoqing Dong
    Mayo Clinic Scottsdale, 13400 East Shea Blvd, Scottsdale, AZ 85259, USA
    Ann N Y Acad Sci 1070:243-7. 2006
    ..These data provide an important constraint for modeling the agonist-bound G protein-coupled secretin receptor and should add substantially to our current understanding of the molecular basis of ligand binding of this important receptor...
  6. pmc Juxtamembranous region of the amino terminus of the family B G protein-coupled calcitonin receptor plays a critical role in small-molecule agonist action
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    J Biol Chem 284:21839-47. 2009
    ..These findings focus attention on the potential importance of the juxtamembranous region of the amino terminus of the Family B calcitonin receptor for agonist drug action...
  7. ncbi request reprint Molecular approximation between a residue in the amino-terminal region of calcitonin and the third extracellular loop of the class B G protein-coupled calcitonin receptor
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    J Biol Chem 279:31177-82. 2004
    ..These data are consistent with a common ligand binding mechanism for receptors in this important family...
  8. pmc Mapping spatial approximations between the amino terminus of secretin and each of the extracellular loops of its receptor using cysteine trapping
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    FASEB J 26:5092-105. 2012
    ..Key spatial approximations between peptide and receptor predicted by this model (H(1)-W(274), D(3)-N(268), G(4)-F(258)) were supported by mutagenesis and residue-residue complementation studies...
  9. pmc Functional importance of a structurally distinct homodimeric complex of the family B G protein-coupled secretin receptor
    Fan Gao
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, AZ 85259, USA
    Mol Pharmacol 76:264-74. 2009
    ..We propose the existence of a functionally important, structurally specific high-affinity dimeric state of the secretin receptor, which may be typical of family B G protein-coupled receptors...
  10. doi request reprint Spatial approximation between secretin residue five and the third extracellular loop of its receptor provides new insight into the molecular basis of natural agonist binding
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 13400 East Shea Blvd, Scottsdale, AZ 85259, USA
    Mol Pharmacol 74:413-22. 2008
    ....
  11. pmc Molecular basis of secretin docking to its intact receptor using multiple photolabile probes distributed throughout the pharmacophore
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    J Biol Chem 286:23888-99. 2011
    ....
  12. ncbi request reprint Spatial approximation between two residues in the mid-region of secretin and the amino terminus of its receptor. Incorporation of seven sets of such constraints into a three-dimensional model of the agonist-bound secretin receptor
    Maoqing Dong
    Cancer Center and the Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    J Biol Chem 278:48300-12. 2003
    ..This provides clear insights into the molecular basis of natural ligand binding and supplies testable hypotheses regarding the molecular basis of activation of this receptor...
  13. pmc Secretin occupies a single protomer of the homodimeric secretin receptor complex: insights from photoaffinity labeling studies using dual sites of covalent attachment
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    J Biol Chem 285:9919-31. 2010
    ..These data are consistent with a model of secretin occupying a single secretin receptor protomer within the homodimeric receptor complex. A new molecular model accommodating all constraints is now proposed...
  14. ncbi request reprint Molecular approximations between residues 21 and 23 of secretin and its receptor: development of a model for peptide docking with the amino terminus of the secretin receptor
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic Scottsdale, Scottsdale, Arizona, USA
    Mol Pharmacol 72:280-90. 2007
    ..This model was found to fully accommodate all existing constraints, as well as the two new approximations identified in this work...
  15. ncbi request reprint Fluorescence resonance energy transfer analysis of secretin docking to its receptor: mapping distances between residues distributed throughout the ligand pharmacophore and distinct receptor residues
    Kaleeckal G Harikumar
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 13400 E Shea Boulevard, Scottsdale, AZ 85259, USA
    J Biol Chem 282:32834-43. 2007
    ..This provides strong evidence for the orientation of peptide-binding and signaling domains of a prototypic Class II G protein-coupled receptor...
  16. pmc Elucidation of the molecular basis of cholecystokinin Peptide docking to its receptor using site-specific intrinsic photoaffinity labeling and molecular modeling
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    Biochemistry 48:5303-12. 2009
    ..The resultant agonist ligand-occupied receptor models fully accommodate all existing experimental data and represent the best refined models of a peptide hormone receptor in this important family...
  17. ncbi request reprint Spatial approximation between the amino terminus of a peptide agonist and the top of the sixth transmembrane segment of the secretin receptor
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    J Biol Chem 279:2894-903. 2004
    ..This suggests that secretin binding may exert tension between the receptor amino terminus and the transmembrane domain to elicit a conformational change effecting receptor activation...
  18. pmc Role of lysine187 within the second extracellular loop of the type A cholecystokinin receptor in agonist-induced activation. Use of complementary charge-reversal mutagenesis to define a functionally important interdomain interaction
    Maoqing Dong
    Cancer Center and Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    Biochemistry 46:4522-31. 2007
    ..This residue-residue interaction is nicely accommodated within a new molecular model of the agonist-occupied cholecystokinin receptor...
  19. pmc Spatial approximations between residues 6 and 12 in the amino-terminal region of glucagon-like peptide 1 and its receptor: a region critical for biological activity
    Quan Chen
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    J Biol Chem 285:24508-18. 2010
    ..This region of interaction of peptide amino-terminal domains with the receptor may provide a pocket that can be targeted by small molecule agonists...
  20. pmc Lactam constraints provide insights into the receptor-bound conformation of secretin and stabilize a receptor antagonist
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    Biochemistry 50:8181-92. 2011
    ..This lactam bridge is believed to stabilize an extended α-helical conformation of this peptide while in solution and not to interfere with critical residue-residue approximations while docked to the receptor...
  21. pmc Glucagon-like peptide-1 receptor dimerization differentially regulates agonist signaling but does not affect small molecule allostery
    Kaleeckal G Harikumar
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, AZ 85259, USA
    Proc Natl Acad Sci U S A 109:18607-12. 2012
    ....
  22. pmc Importance of each residue within secretin for receptor binding and biological activity
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, United States
    Biochemistry 50:2983-93. 2011
    ..This could reflect reduced contact with the receptor or an increase in the solvent-accessible surface area of the hydrophobic residues in the carboxyl terminus of secretin as bound to its receptor...
  23. ncbi request reprint Differential docking of high-affinity peptide ligands to type A and B cholecystokinin receptors demonstrated by photoaffinity labeling
    Maoqing Dong
    Mayo Clinic Scottsdale, Cancer Center and Department of Molecular Pharmacology and Experimental Therapeutics, Scottsdale, Arizona 85259, USA
    Biochemistry 44:6693-700. 2005
    ..This provides a direct demonstration of distinct modes of docking the same high-affinity ligand to highly homologous receptors...
  24. ncbi request reprint Paired cysteine mutagenesis to establish the pattern of disulfide bonds in the functional intact secretin receptor
    Cayle S Lisenbee
    Cancer Center and Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    J Biol Chem 280:12330-8. 2005
    ..In conclusion, these data suggest the same pattern of disulfide bonding as that predicted previously by NMR and thus support a consistent pattern of amino-terminal disulfide bonds in class B G protein-coupled receptors...
  25. pmc Molecular basis of glucagon-like peptide 1 docking to its intact receptor studied with carboxyl-terminal photolabile probes
    Quan Chen
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    J Biol Chem 284:34135-44. 2009
    ..This should expand our understanding of the molecular basis of natural agonist ligand binding to the GLP1 receptor and may be relevant to other family members...
  26. pmc Elucidation of the active conformation of the amino terminus of receptor-bound secretin using intramolecular disulfide bond constraints
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, AZ 85259, USA
    Bioorg Med Chem Lett 20:6040-4. 2010
    ..It will be critical to extend similar studies to other family members to learn what structural elements might be most conserved in this family...
  27. ncbi request reprint Insights into the structure and molecular basis of ligand docking to the G protein-coupled secretin receptor using charge-modified amino-terminal agonist probes
    Maoqing Dong
    M D Director, Cancer Center Mayo Clinic in Scottsdale, 13400 East Shea Boulevard, Johnson Research Building, Scottsdale AZ 85259, USA
    Mol Endocrinol 19:1821-36. 2005
    ..This supports the possibility that there is a charge-charge interaction between this residue and the amino terminus of secretin that is critical to its normal docking...
  28. pmc Refinement of the pharmacophore of an agonist ligand of the secretin receptor using conformationally constrained cyclic hexapeptides
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, AZ 85259, United States
    Peptides 31:1094-8. 2010
    ..Molecular modeling supported the presence of a unique WD backbone conformation shared only by these active peptides, and provided a more constrained template for future receptor-active agonist drug development...
  29. pmc Role of N-linked glycosylation in biosynthesis, trafficking, and function of the human glucagon-like peptide 1 receptor
    Quan Chen
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 13400 East Shea Blvd, Scottsdale, AZ 85259, USA
    Am J Physiol Endocrinol Metab 299:E62-8. 2010
    ..These data suggest that N-linked glycosylation of the GLP-1 receptor is important for its normal folding and trafficking to the cell surface...
  30. ncbi request reprint Differential spatial approximation between secretin and its receptor residues in active and inactive conformations demonstrated by photoaffinity labeling
    Maoqing Dong
    Cancer Center and Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    Mol Endocrinol 20:1688-98. 2006
    ....
  31. ncbi request reprint Possible endogenous agonist mechanism for the activation of secretin family G protein-coupled receptors
    Maoqing Dong
    Mayo Clinic, 13400 E Shea Boulevard, Scottsdale, AZ 85259, USA
    Mol Pharmacol 70:206-13. 2006
    ..These data may provide a unique molecular mechanism and novel leads for the development of small-molecule agonists acting at potential drug targets within this physiologically important receptor family...
  32. pmc Refinement of glucagon-like peptide 1 docking to its intact receptor using mid-region photolabile probes and molecular modeling
    Laurence J Miller
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    J Biol Chem 286:15895-907. 2011
    ..Establishing ligand residue approximation with this loop region is unique among family members and may help to orient the receptor amino-terminal domain relative to its helical bundle region...
  33. ncbi request reprint Key differences in molecular complexes of the cholecystokinin receptor with structurally related peptide agonist, partial agonist, and antagonist
    Sonnet J H Arlander
    Cancer Center, Mayo Clinic in Scottsdale, 13400 East Shea Boulevard, Johnson Research Building, Scottsdale AZ 85259, USA
    Mol Pharmacol 66:545-52. 2004
    ..Of note, the analogous antagonist probe labeled a distinct region within the receptor amino terminus, confirming a key structural difference in active and inactive complexes...
  34. pmc Transmembrane segment peptides can disrupt cholecystokinin receptor oligomerization without affecting receptor function
    Kaleeckal G Harikumar
    Cancer Center and Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    Biochemistry 45:14706-16. 2006
    ....
  35. ncbi request reprint Spatial approximation between a photolabile residue in position 13 of secretin and the amino terminus of the secretin receptor
    Mengwei Zang
    Cancer Center and the Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    Mol Pharmacol 63:993-1001. 2003
    ..Together, these five pairs of residue-residue approximations provide important constraints to better understand the molecular conformation of the agonist-bound receptor...
  36. ncbi request reprint Distinct molecular mechanisms for agonist peptide binding to types A and B cholecystokinin receptors demonstrated using fluorescence spectroscopy
    Kaleeckal G Harikumar
    Cancer Center and Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic Scottsdale, Scottsdale, Arizona 85259, USA
    J Biol Chem 280:1044-50. 2005
    ..Of interest, despite this difference in binding, activation of both receptors results in analogous direction of movement of the fluorescent indicator probes...
  37. ncbi request reprint Functional characterization and purification of the secretin receptor expressed in baculovirus-infected insect cells
    Yan W Asmann
    Cancer Center and Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic Scottsdale, Johnson Research Building, Scottsdale, AZ 85259, USA
    Regul Pept 123:217-23. 2004
    ..This expression system should facilitate the structural characterization of this receptor and its important amino-terminal domain...
  38. ncbi request reprint Demonstration of a specific site of covalent labeling of the human motilin receptor using a biologically active photolabile motilin analog
    Bunzo Matsuura
    Cancer Center and Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic in Scottsdale, AZ 85259, USA
    J Pharmacol Exp Ther 313:1101-8. 2005
    ....
  39. pmc Differential determinants for coupling of distinct G proteins with the class B secretin receptor
    Gene L Garcia
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, AZ 85259, USA
    Am J Physiol Cell Physiol 302:C1202-12. 2012
    ..These data suggest that, while some receptor determinants are clearly shared, there are also distinct determinants for coupling with each of these G proteins...
  40. pmc Ligand binding and activation of the secretin receptor, a prototypic family B G protein-coupled receptor
    Laurence J Miller
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, AZ, USA
    Br J Pharmacol 166:18-26. 2012
    ..This complex is important for facilitating G protein association and achieving the high affinity state of this receptor...
  41. pmc Molecular basis for binding and subtype selectivity of 1,4-benzodiazepine antagonist ligands of the cholecystokinin receptor
    Erin E Cawston
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, Arizona 85259, USA
    J Biol Chem 287:18618-35. 2012
    ..Furthermore, the strong predictive power of these models suggests their usefulness in the discovery of lead compounds and in drug development programs...
  42. ncbi request reprint Use of photoaffinity labeling to understand the molecular basis of ligand binding to the secretin receptor
    Maoqing Dong
    Mayo Clinic, 13400 East Shea Blvd, Scottsdale, AZ 85259, USA
    Ann N Y Acad Sci 1070:248-64. 2006
    ..Each of the themes discussed are also relevant to other members of this physiologically and pharmacologically important receptor family...
  43. pmc Insights into the impact of phenolic residue incorporation at each position along secretin for receptor binding and biological activity
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, Scottsdale, AZ 85259, United States
    Regul Pept 180:5-11. 2013
    ..As the structure of the intact receptor is elucidated, these data will provide a guide for ligand docking to the core domain and to help clarify the molecular basis of receptor activation...
  44. pmc Predicting the effects of amino acid replacements in peptide hormones on their binding affinities for class B GPCRs and application to the design of secretin receptor antagonists
    Jerez A Te
    Mayo Clinic, 13400 East Shea Boulevard, Scottsdale, AZ 85259, USA
    J Comput Aided Mol Des 26:835-45. 2012
    ..These simulations suggested that a combination of the α-helical propensity of the unbound peptide and specific interactions between the peptide and the receptor extracellular domain contribute to their higher binding affinities...
  45. ncbi request reprint Differential spatial approximation between cholecystokinin residue 30 and receptor residues in active and inactive conformations
    Maoqing Dong
    Cancer Center, Mayo Clinic Scottsdale, 13400 East Shea Blvd, Scottsdale, AZ 85259, USA
    Mol Pharmacol 67:1892-900. 2005
    ..These data extend our understanding of the molecular basis of binding and the conformational states of this important receptor...
  46. ncbi request reprint Molecular pharmacology of the secretin receptor
    Maoqing Dong
    Center for Basic Research in Digestive Diseases, Departments of Internal Medicine and Biochemistry Molecular Biology, Mayo Clinic and Foundation, Guggenheim 17, Mayo Clinic, Rochester, MN 55905, USA
    Receptors Channels 8:189-200. 2002
    ..This receptor is regulated by agonist-stimulated phosphorylation and internalization, with details dependent on the cellular environment...
  47. ncbi request reprint Biochemical and cell biological mechanisms of cholecystokinin receptor regulation
    Laurence J Miller
    Mayo Clinic, Department of Molecular Pharmacology and Experimental Therapeutics, Scottsdale, Arizona 85259, USA
    Curr Top Med Chem 7:1166-72. 2007
    ..Agonist occupation results in oligomer dissociation, but the functional significance of this observation has yet to be defined...
  48. doi request reprint The orthosteric agonist-binding pocket in the prototypic class B G-protein-coupled secretin receptor
    Laurence J Miller
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 13400 East Shea Boulevard, Scottsdale, AZ 85259, USA
    Biochem Soc Trans 41:154-8. 2013
    ..Such insights will be useful in the rational development of drugs acting at this important group of targets...
  49. pmc Effects of pH and temperature on photoaffinity labeling of Family B G protein-coupled receptors
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 13400 East Shea Boulevard, Scottsdale, AZ 85259, USA
    Regul Pept 158:110-5. 2009
    ..Application to the calcitonin receptor, another Family B G protein-coupled receptor, yielded analogous results. These results support the consistent mode of docking peptide ligands to this group of receptors...
  50. ncbi request reprint A novel secretin receptor splice variant potentially useful for early diagnosis of pancreatic carcinoma
    Gregory M Hayes
    Mayo Clinic, Cancer Center and Department of Molecular Pharmacology and Experimental Therapeutics, Scottsdale, Arizona 85259, USA
    Gastroenterology 133:853-61. 2007
    ..With premessenger RNA splicing abnormalities common in cancer, we evaluated whether an abnormal secretin receptor spliceoform were present, characterized it, and developed a serum assay for it...
  51. pmc Direct demonstration of unique mode of natural peptide binding to the type 2 cholecystokinin receptor using photoaffinity labeling
    Maoqing Dong
    Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic Scottsdale, Scottsdale, AZ 85259, United States
    Peptides 46:143-9. 2013
    ..This was in contrast to its covalent attachment to Glu(345) in the third extracellular loop of the type 1 CCK receptor, directly documenting differences in mode of docking this peptide to these receptors. ..
  52. ncbi request reprint Development of a biologically active secretin analogue incorporating a radioiodinatable photolabile p-(4-hydroxybenzoyl)phenylalanine in position 10
    Maoqing Dong
    Center for Basic Research in Digestive Diseases, Department of Internal Medicine, Mayo Clinic and Foundation, Rochester, MN 55905, USA
    Regul Pept 109:181-7. 2002
    ....
  53. ncbi request reprint Insights into interactions between the alpha-helical region of the salmon calcitonin antagonists and the human calcitonin receptor using photoaffinity labeling
    Vi Pham
    Howard Florey Institute, The University of Melbourne, Victoria 3010, Australia
    J Biol Chem 280:28610-22. 2005
    ..The model was also consistent with cooperative interaction between the receptor amino terminus and the receptor core...
  54. ncbi request reprint Differential contributions of motilin receptor extracellular domains for peptide and non-peptidyl agonist binding and activity
    Bunzo Matsuura
    Third Department of Internal Medicine, Ehime University School of Medicine, Shitsukawa 454, Tohon, Ehime 791 0295, Japan
    J Biol Chem 281:12390-6. 2006
    ....
  55. ncbi request reprint Interaction among four residues distributed through the secretin pharmacophore and a focused region of the secretin receptor amino terminus
    Maoqing Dong
    Center for Basic Research in Digestive Diseases, Department of Internal Medicine, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
    Mol Endocrinol 16:2490-501. 2002
    ..Additional experimentally derived constraints will be necessary to determine the spatial positioning of this complex with the remainder of the SecR...
  56. ncbi request reprint Mutational analysis of predicted intracellular loop domains of human motilin receptor
    Hitoo Tokunaga
    Department of Gastroenterology and Metabology, Ehime University Graduate School of Medicine, Shitsukawa 454, Tohon, Ehime 791 0295, Japan
    Am J Physiol Gastrointest Liver Physiol 294:G460-6. 2008
    ..The identification of functionally important residues in the predicted cytosolic face provides strong candidates for playing roles in receptor-G protein interaction...
  57. ncbi request reprint Differential determinants for peptide and non-peptidyl ligand binding to the motilin receptor. Critical role of second extracellular loop for peptide binding and action
    Bunzo Matsuura
    Center for Basic Research in Digestive Diseases, Department of Internal Medicine, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
    J Biol Chem 277:9834-9. 2002
    ....
  58. ncbi request reprint Forskolin suppresses insulin gene transcription in islet beta-cells through a protein kinase A-independent pathway
    Wei Qun Ding
    Department of Medicine, Center for Basic Research in Digestive Diseases, Mayo Clinic and Foundation, Guggenheim 17, Rochester, MN 55905, USA
    Cell Signal 15:27-35. 2003
    ..These results demonstrate that forskolin suppresses insulin transcription in INS-1 cells through a PKA-independent mechanism that probably involves MAP kinase signalling...
  59. ncbi request reprint Molecular characterization and distribution of motilin family receptors in the human gastrointestinal tract
    Eiji Takeshita
    Third Department of Internal Medicine, Ehime University School of Medicine, Shitsukawa 454, Toon, 791 0295, Japan
    J Gastroenterol 41:223-30. 2006
    ..The aims of this study were to explore the distribution of motilin and ghrelin receptors along the human gastrointestinal tract and to establish the molecular nature of the human motilin receptor...