Karl J Clark

Summary

Affiliation: Mayo Clinic
Country: USA

Publications

  1. pmc Stressing zebrafish for behavioral genetics
    Karl J Clark
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55901, USA
    Rev Neurosci 22:49-62. 2011
  2. pmc Trapping cardiac recessive mutants via expression-based insertional mutagenesis screening
    Yonghe Ding
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Circ Res 112:606-17. 2013
  3. pmc High efficiency In Vivo genome engineering with a simplified 15-RVD GoldyTALEN design
    Alvin C Ma
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota, USA
    PLoS ONE 8:e65259. 2013
  4. pmc Transgenic zebrafish using transposable elements
    Karl J Clark
    Mayo Clinic, Department of Biochemistry and Molecular Biology, Mayo Addiction Research Center, Rochester, Minnesota, USA
    Methods Cell Biol 104:137-49. 2011
  5. pmc Mojo Hand, a TALEN design tool for genome editing applications
    Kevin L Neff
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN, USA
    BMC Bioinformatics 14:1. 2013
  6. pmc The CRISPR system--keeping zebrafish gene targeting fresh
    Patrick R Blackburn
    Department of Biochemistry and Molecular Biology, Mayo Addiction Research Center, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Zebrafish 10:116-8. 2013
  7. pmc In vivo genome editing using a high-efficiency TALEN system
    Victoria M Bedell
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA
    Nature 491:114-8. 2012
  8. pmc zfishbook: connecting you to a world of zebrafish revertible mutants
    Karl J Clark
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
    Nucleic Acids Res 40:D907-11. 2012
  9. pmc The lineage-specific gene ponzr1 is essential for zebrafish pronephric and pharyngeal arch development
    Victoria M Bedell
    Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Development 139:793-804. 2012
  10. pmc In vivo protein trapping produces a functional expression codex of the vertebrate proteome
    Karl J Clark
    Mayo Clinic, Rochester, Minnesota, USA
    Nat Methods 8:506-15. 2011

Collaborators

Detail Information

Publications16

  1. pmc Stressing zebrafish for behavioral genetics
    Karl J Clark
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55901, USA
    Rev Neurosci 22:49-62. 2011
    ..Understanding the genetic and epigenetic basis for the stress response in vertebrates will help to develop advanced screening and therapies for stress-aggravated diseases such as addiction and mood and anxiety disorders...
  2. pmc Trapping cardiac recessive mutants via expression-based insertional mutagenesis screening
    Yonghe Ding
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Circ Res 112:606-17. 2013
    ..However, the increased colony management efforts in vertebrates impose a significant challenge for identifying genes affecting a particular organ, such as the heart, especially those exhibiting adult phenotypes on depletion...
  3. pmc High efficiency In Vivo genome engineering with a simplified 15-RVD GoldyTALEN design
    Alvin C Ma
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota, USA
    PLoS ONE 8:e65259. 2013
    ..In conclusion, these high efficiency 15-RVD GoldyTALENs are useful for high-throughput mutagenesis in diverse application including hypothesis testing from basic science to precision medicine...
  4. pmc Transgenic zebrafish using transposable elements
    Karl J Clark
    Mayo Clinic, Department of Biochemistry and Molecular Biology, Mayo Addiction Research Center, Rochester, Minnesota, USA
    Methods Cell Biol 104:137-49. 2011
    ..Together, these data should enable the ready generation of transgenic zebrafish for scientific inquiry...
  5. pmc Mojo Hand, a TALEN design tool for genome editing applications
    Kevin L Neff
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN, USA
    BMC Bioinformatics 14:1. 2013
    ....
  6. pmc The CRISPR system--keeping zebrafish gene targeting fresh
    Patrick R Blackburn
    Department of Biochemistry and Molecular Biology, Mayo Addiction Research Center, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Zebrafish 10:116-8. 2013
    ..Together, these tools will help overcome many of the bottlenecks that have constrained gene targeting in zebrafish, paving the way for advanced genome engineering applications in this model teleost...
  7. pmc In vivo genome editing using a high-efficiency TALEN system
    Victoria M Bedell
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA
    Nature 491:114-8. 2012
    ..We further show successful germline transmission of both EcoRV and mloxP engineered chromosomes. This combined approach offers the potential to model genetic variation as well as to generate targeted conditional alleles...
  8. pmc zfishbook: connecting you to a world of zebrafish revertible mutants
    Karl J Clark
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
    Nucleic Acids Res 40:D907-11. 2012
    ....
  9. pmc The lineage-specific gene ponzr1 is essential for zebrafish pronephric and pharyngeal arch development
    Victoria M Bedell
    Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Development 139:793-804. 2012
    ..Together, this work provides experimental evidence of an additional mechanism that incorporates evolutionarily dynamic, lineage-specific gene families into conserved regulatory gene networks to create functional organ diversity...
  10. pmc In vivo protein trapping produces a functional expression codex of the vertebrate proteome
    Karl J Clark
    Mayo Clinic, Rochester, Minnesota, USA
    Nat Methods 8:506-15. 2011
    ..The RP2 mutagenesis system is a step toward a unified 'codex' of protein expression and direct functional annotation of the vertebrate genome...
  11. pmc An in vivo method to quantify lymphangiogenesis in zebrafish
    Scott J Hoffman
    Division of Cardiovascular Diseases, Mayo Clinic, Rochester, Minnesota, USA
    PLoS ONE 7:e45240. 2012
    ..Identification of a network of trunk lymphatic capillaries in zebrafish provides an opportunity to quantify lymphatic growth in vivo...
  12. pmc Revealing the role of phospholipase Cβ3 in the regulation of VEGF-induced vascular permeability
    Luke H Hoeppner
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Blood 120:2167-73. 2012
    ..Our results suggest an important effect of PLCβ3 on VP and provide a new model with which to identify genetic regulators of VP crucial to several disease processes...
  13. pmc Functional analysis of slow myosin heavy chain 1 and myomesin-3 in sarcomere organization in zebrafish embryonic slow muscles
    Jin Xu
    Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21202, USA
    J Genet Genomics 39:69-80. 2012
    ..Together, these studies indicate that myosin thick filaments are required for M-line organization and M-line localization of myomesin-3. In contrast, myomesin-3 is dispensable for sarcomere organization in slow muscles...
  14. pmc Zebrafish: a model for the study of addiction genetics
    Eric W Klee
    Mayo Addiction Research Center, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
    Hum Genet 131:977-1008. 2012
    ..Expansion of investigations into drug response using model organisms holds the potential to advance our understanding of drug response and addiction in humans...
  15. pmc A TALE of two nucleases: gene targeting for the masses?
    Karl J Clark
    Department of Biochemistry and Molecular Biology, Mayo Addiction Research Center, Mayo Clinic, Rochester, Minnesota 55905, USA
    Zebrafish 8:147-9. 2011
    ..TALEN construction is simpler, potentially more reliable, and in the few cases examined, shows fewer off-target effects than corresponding ZFNs. TALENs promise to bring gene targeting to the majority of zebrafish laboratories...
  16. pmc Transposon tools hopping in vertebrates
    Jun Ni
    Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
    Brief Funct Genomic Proteomic 7:444-53. 2008
    ..We have summarized the major transposon vector systems active in vertebrates, comparing and contrasting known critical biochemical and in vivo properties, for future tool design and new genetic applications...