Research Topics
Genomes and Genes | Karl J ClarkSummaryAffiliation: Mayo Clinic Country: USA Publications
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Publications
Stressing zebrafish for behavioral geneticsKarl J Clark
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55901, USA
Rev Neurosci 22:49-62. 2011..Understanding the genetic and epigenetic basis for the stress response in vertebrates will help to develop advanced screening and therapies for stress-aggravated diseases such as addiction and mood and anxiety disorders...- Transgenic zebrafish using transposable elementsKarl J Clark
Mayo Clinic, Department of Biochemistry and Molecular Biology, Mayo Addiction Research Center, Rochester, Minnesota, USA
Methods Cell Biol 104:137-49. 2011..Together, these data should enable the ready generation of transgenic zebrafish for scientific inquiry... - In vivo genome editing using a high-efficiency TALEN systemVictoria M Bedell
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905, USA
Nature 491:114-8. 2012..We further show successful germline transmission of both EcoRV and mloxP engineered chromosomes. This combined approach offers the potential to model genetic variation as well as to generate targeted conditional alleles... - zfishbook: connecting you to a world of zebrafish revertible mutantsKarl J Clark
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
Nucleic Acids Res 40:D907-11. 2012.... - The lineage-specific gene ponzr1 is essential for zebrafish pronephric and pharyngeal arch developmentVictoria M Bedell
Department of Biochemistry and Molecular Biology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
Development 139:793-804. 2012..Together, this work provides experimental evidence of an additional mechanism that incorporates evolutionarily dynamic, lineage-specific gene families into conserved regulatory gene networks to create functional organ diversity... - Mojo Hand, a TALEN design tool for genome editing applicationsKevin L Neff
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN, USA
BMC Bioinformatics 14:1. 2013.... - In vivo protein trapping produces a functional expression codex of the vertebrate proteomeKarl J Clark
Mayo Clinic, Rochester, Minnesota, USA
Nat Methods 8:506-15. 2011..The RP2 mutagenesis system is a step toward a unified 'codex' of protein expression and direct functional annotation of the vertebrate genome... - An in vivo method to quantify lymphangiogenesis in zebrafishScott J Hoffman
Division of Cardiovascular Diseases, Mayo Clinic, Rochester, Minnesota, USA
PLoS ONE 7:e45240. 2012..Identification of a network of trunk lymphatic capillaries in zebrafish provides an opportunity to quantify lymphatic growth in vivo... - Trapping cardiac recessive mutants via expression-based insertional mutagenesis screeningYonghe Ding
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Circ Res 112:606-17. 2013..However, the increased colony management efforts in vertebrates impose a significant challenge for identifying genes affecting a particular organ, such as the heart, especially those exhibiting adult phenotypes on depletion... - Revealing the role of phospholipase Cβ3 in the regulation of VEGF-induced vascular permeabilityLuke H Hoeppner
Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
Blood 120:2167-73. 2012..Our results suggest an important effect of PLCβ3 on VP and provide a new model with which to identify genetic regulators of VP crucial to several disease processes... 
Zebrafish: a model for the study of addiction geneticsEric W Klee
Mayo Addiction Research Center, Mayo Clinic, 200 First Street SW, Rochester, MN 55905, USA
Hum Genet 131:977-1008. 2012..Expansion of investigations into drug response using model organisms holds the potential to advance our understanding of drug response and addiction in humans...- Functional analysis of slow myosin heavy chain 1 and myomesin-3 in sarcomere organization in zebrafish embryonic slow musclesJin Xu
Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, MD 21202, USA
J Genet Genomics 39:69-80. 2012..Together, these studies indicate that myosin thick filaments are required for M-line organization and M-line localization of myomesin-3. In contrast, myomesin-3 is dispensable for sarcomere organization in slow muscles... - A TALE of two nucleases: gene targeting for the masses?Karl J Clark
Department of Biochemistry and Molecular Biology, Mayo Addiction Research Center, Mayo Clinic, Rochester, Minnesota 55905, USA
Zebrafish 8:147-9. 2011..TALEN construction is simpler, potentially more reliable, and in the few cases examined, shows fewer off-target effects than corresponding ZFNs. TALENs promise to bring gene targeting to the majority of zebrafish laboratories... - Transposon tools hopping in vertebratesJun Ni
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905, USA
Brief Funct Genomic Proteomic 7:444-53. 2008..We have summarized the major transposon vector systems active in vertebrates, comparing and contrasting known critical biochemical and in vivo properties, for future tool design and new genetic applications...