THOMAS BURGHARDT

Summary

Affiliation: Mayo Clinic
Country: USA

Publications

  1. ncbi request reprint Cation-pi interaction in a folded polypeptide
    Thomas P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Foundation, 200 First St SW, Rochester, MN 55905, USA
    Biopolymers 63:261-72. 2002
  2. ncbi request reprint Tyrosine mediated tryptophan ATP sensitivity in skeletal myosin
    Sungjo Park
    Department of Biochemistry and Molecular Biology, Mayo Foundation, 200 First Street Southwest, Rochester, Minnesota 55905, USA
    Biochemistry 41:1436-44. 2002
  3. ncbi request reprint In situ single-molecule imaging with attoliter detection using objective total internal reflection confocal microscopy
    Thomas P Burghardt
    Department of Physiology and Biomedical Imaging, Mayo Clinic, Rochester, Minnesota 55905, USA
    Biochemistry 45:4058-68. 2006
  4. pmc Around-the-objective total internal reflection fluorescence microscopy
    Thomas P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester, Rochester, Minnesota 55905, USA
    Appl Opt 48:6120-31. 2009
  5. pmc Regulatory light chain mutants linked to heart disease modify the cardiac myosin lever arm
    Thomas P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester, Rochester, MN 55905, USA
    Biochemistry 52:1249-59. 2013
  6. pmc Measuring incidence angle for through-the-objective total internal reflection fluorescence microscopy
    Thomas P Burghardt
    Mayo Clinic Rochester, Department of Biochemistry and Molecular Biology, Rochester, Minnesota 55905, USA
    J Biomed Opt 17:126007. 2012
  7. pmc Evanescent field shapes excitation profile under axial epi-illumination
    Thomas P Burghardt
    Mayo Clinic Rochester, Department of Biochemistry and Molecular Biology, Rochester, Minnesota 55905, USA
    J Biomed Opt 17:066021. 2012
  8. pmc GFP-tagged regulatory light chain monitors single myosin lever-arm orientation in a muscle fiber
    Thomas P Burghardt
    Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Biophys J 93:2226-39. 2007
  9. pmc Myosin dynamics on the millisecond time scale
    Thomas P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Biophys Chem 131:15-28. 2007
  10. pmc Single myosin lever arm orientation in a muscle fiber detected with photoactivatable GFP
    Thomas P Burghardt
    Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Biochemistry 48:754-65. 2009

Research Grants

  1. In Situ Sensing of Single Myosin Function in Hypertrophy Disease
    Thomas P Burghardt; Fiscal Year: 2010
  2. MOLECULAR ANGLE TRANSITIONS IN MUSCLE
    THOMAS BURGHARDT; Fiscal Year: 2001
  3. Visualizing Actomyosin Transients by Data Merging
    THOMAS BURGHARDT; Fiscal Year: 2007
  4. Visualizing Actomyosin Transients by Data Merging
    Thomas P Burghardt; Fiscal Year: 2010
  5. MOLECULAR ANGLE TRANSITIONS IN MUSCLE
    THOMAS BURGHARDT; Fiscal Year: 1993
  6. MOLECULAR ANGLE TRANSITIONS IN MUSCLE
    THOMAS BURGHARDT; Fiscal Year: 1992

Collaborators

Detail Information

Publications33

  1. ncbi request reprint Cation-pi interaction in a folded polypeptide
    Thomas P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Foundation, 200 First St SW, Rochester, MN 55905, USA
    Biopolymers 63:261-72. 2002
    ..The ensemble average spectroscopic signature effects in t6p are small and in agreement with observation...
  2. ncbi request reprint Tyrosine mediated tryptophan ATP sensitivity in skeletal myosin
    Sungjo Park
    Department of Biochemistry and Molecular Biology, Mayo Foundation, 200 First Street Southwest, Rochester, Minnesota 55905, USA
    Biochemistry 41:1436-44. 2002
    ..Using crystallographically based geometry, calculation of the Trp510 electronic wave function indicates that Tyr503 is the static quencher...
  3. ncbi request reprint In situ single-molecule imaging with attoliter detection using objective total internal reflection confocal microscopy
    Thomas P Burghardt
    Department of Physiology and Biomedical Imaging, Mayo Clinic, Rochester, Minnesota 55905, USA
    Biochemistry 45:4058-68. 2006
    ..Muscle tissue contains myosin at a approximately 120 microM concentration. Evidence is provided that >75% of the bleachable fluorescence detected with fTIRM is emitted by five chromophore-labeled myosins in a muscle fiber...
  4. pmc Around-the-objective total internal reflection fluorescence microscopy
    Thomas P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester, Rochester, Minnesota 55905, USA
    Appl Opt 48:6120-31. 2009
    ..The approach eliminates background light due to the admission of the laser excitation to the microscopic optics while collecting near-field emission from the dipoles excited by the approximately 50 nm deep evanescent field...
  5. pmc Regulatory light chain mutants linked to heart disease modify the cardiac myosin lever arm
    Thomas P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester, Rochester, MN 55905, USA
    Biochemistry 52:1249-59. 2013
    ..A lower free energy intermediate occupancy involves a lower energy conversion efficiency in the fiber relating a specific myosin function modification to the disease-implicated mutant...
  6. pmc Measuring incidence angle for through-the-objective total internal reflection fluorescence microscopy
    Thomas P Burghardt
    Mayo Clinic Rochester, Department of Biochemistry and Molecular Biology, Rochester, Minnesota 55905, USA
    J Biomed Opt 17:126007. 2012
    ..Calibration depends on microscope characteristics and TIRF objective and is applicable to any interface or sample...
  7. pmc Evanescent field shapes excitation profile under axial epi-illumination
    Thomas P Burghardt
    Mayo Clinic Rochester, Department of Biochemistry and Molecular Biology, Rochester, Minnesota 55905, USA
    J Biomed Opt 17:066021. 2012
    ..The goal is to develop and verify the practical depiction of excitation and emission in a real microscope for quantitative interpretation of the 3-D emission pattern...
  8. pmc GFP-tagged regulatory light chain monitors single myosin lever-arm orientation in a muscle fiber
    Thomas P Burghardt
    Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, 200 First Street SW, Rochester, MN 55905, USA
    Biophys J 93:2226-39. 2007
    ..The GFP-tagged RLC is a novel probe to assess single-lever-arm orientation characteristics in situ...
  9. pmc Myosin dynamics on the millisecond time scale
    Thomas P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, MN 55905, USA
    Biophys Chem 131:15-28. 2007
    ....
  10. pmc Single myosin lever arm orientation in a muscle fiber detected with photoactivatable GFP
    Thomas P Burghardt
    Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Biochemistry 48:754-65. 2009
    ..P., et al. (2007) Biophys. J. 93, 2226]. The latter may have a role in contraction because it provides additional free energy favoring completion of the cross-bridge power stroke...
  11. pmc Mapping microscope object polarized emission to the back focal plane pattern
    Thomas P Burghardt
    Mayo Clinic Rochester, Department of Biochemistry, 200 First Street South West, Rochester, Minnesota 55905, USA
    J Biomed Opt 14:034036. 2009
    ..TIR/BFP pattern imaging requires a simple alteration of the fluorescence microscope and is consistent with single-molecule imaging in a fluorophore dense three-dimensional object like a muscle fiber...
  12. pmc Myosin individualized: single nucleotide polymorphisms in energy transduction
    Thomas P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester, 200 First Street SW, Rochester, MN 55905, USA
    BMC Genomics 11:172. 2010
    ..In particular, to discover SNP distribution over the conserved sub-domains and surmise what it implies about sub-domain stability and criticality in the energy transduction mechanism...
  13. pmc Single molecule fluorescence image patterns linked to dipole orientation and axial position: application to myosin cross-bridges in muscle fibers
    Thomas P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester, Rochester, Minnesota, United States of America
    PLoS ONE 6:e16772. 2011
    ..They perform in the native biomolecule environment and have been used to detect both probe position and orientation...
  14. pmc Single myosin cross-bridge orientation in cardiac papillary muscle detects lever-arm shear strain in transduction
    Thomas P Burghardt
    Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, United States
    Biochemistry 50:7809-21. 2011
    ..RLC or ELC mutation, should it impact lever-arm shear strain, will be detected as changes in single myosin shear strain or power-stroke substate distribution...
  15. pmc In situ fluorescent protein imaging with metal film-enhanced total internal reflection microscopy
    Thomas P Burghardt
    Department of Physiology and Biomedical Engineering, Mayo Clinic Rochester, Rochester, Minnesota 55905, USA
    Biophys J 90:4662-71. 2006
    ..Reduction of both background fluorescence and detection depth will enhance TIRM's usefulness for single molecule isolation within biological assemblies...
  16. ncbi request reprint The conformation of xanthene dyes in the myosin sulfhydryl one binding site. Part I. Methods and model systems
    T P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, MN 55905, USA
    Biophys Chem 60:119-33. 1996
    ....
  17. ncbi request reprint Fluorescence emission and anisotropy from rhodamine dimers
    T P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Foundation, Rochester, MN 55905, USA
    Biophys Chem 59:119-31. 1996
    ..The rhodamine dimer is an efficient fluorescence emitter with fluorescence anisotropy equivalent to that of the pure monomeric species over the range of excitation wavelengths covering its two lowest energy transitions...
  18. pmc Probes bound to myosin Cys-707 rotate during length transients in contraction
    T P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Foundation, 200 First Street South West, Rochester, MN 55905, USA
    Proc Natl Acad Sci U S A 94:9631-6. 1997
    ..In this investigation we found that closely correlated with such tension changes are changes in the attitude of the rhodamine probes. This correlation strongly supports the conjecture about how shortening is achieved...
  19. ncbi request reprint Conformation of myosin interdomain interactions during contraction: deductions from muscle fibers using polarized fluorescence
    T P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Foundation, 200 First Street SW, Rochester, Minnesota 55905, USA
    Biochemistry 40:4821-33. 2001
    ..Smaller but significant lever arm rotation occurs with ADP dissociation. Structural changes within the S1 interfaces studied are discussed in the accompanying paper [Burghardt et al. (2001) Biochemistry 40, 4834-4843]...
  20. ncbi request reprint Conformation of myosin interdomain interactions during contraction: deductions from proteins in solution
    T P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Foundation, 200 First Street SW, Rochester, Minnesota 55905, USA
    Biochemistry 40:4834-43. 2001
    ..Global structural changes in actomyosin are discussed in the accompanying paper [Burghardt et al. (2001) Biochemistry 40, 4821-4833]...
  21. ncbi request reprint Energy transduction optical sensor in skeletal myosin
    Thomas P Burghardt
    Department of Biochemistry and Molecular Biology, Mayo Foundation, 200 First Street Southwest, Rochester, Minnesota 55905, USA
    Biochemistry 42:5877-84. 2003
    ..Rationalizing the discrepancy invites further investigation of S1 dynamical structure local to the AST during transduction...
  22. ncbi request reprint The myosin cardiac loop participates functionally in the actomyosin interaction
    Katalin Ajtai
    Department of Biochemistry and Molecular Biology, Mayo Clinic Rochester, Rochester, Minnesota 55905, USA
    J Biol Chem 279:23415-21. 2004
    ..It appears likely that the C-loop is an essential electrostatic binding site for actin involved in modulation of actomyosin affinity and regulation of actomyosin ATPase velocity...
  23. pmc The myosin C-loop is an allosteric actin contact sensor in actomyosin
    Katalin Ajtai
    Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    Biochemistry 48:5263-75. 2009
    ..Neither higher surface density nor cardiac tropomyosin-bound actin can accelerate motility velocity in cCl or sCl, suggesting the element initiating maximal myosin activation by actin resides in the C-loop...
  24. ncbi request reprint Agonist-mediated conformational changes in acetylcholine-binding protein revealed by simulation and intrinsic tryptophan fluorescence
    Fan Gao
    Receptor Biology Laboratory, Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester, Minnesota 55905, USA
    J Biol Chem 280:8443-51. 2005
    ..The collective findings showed that ACh initially establishes close register of conserved Trps from adjacent subunits and then draws the C-loop inward to occlude the entrance to the binding cavity...
  25. pmc An unusual transduction pathway in human tonic smooth muscle myosin
    Miriam F Halstead
    Biochemistry and Molecular Biology, Mayo Clinic College of Medicine, Rochester, Minnesota, USA
    Biophys J 93:3555-66. 2007
    ..The exceptional response of W506 to active site conformation opens the possibility that phasic and tonic isoforms differ in how influences from active site ATPase propagate through the protein network...
  26. ncbi request reprint Purification and characterization of two polymorphic variants of short chain acyl-CoA dehydrogenase reveal reduction of catalytic activity and stability of the Gly185Ser enzyme
    Tien V Nguyen
    Department of Medical Genetics, Mayo Clinic and Foundation, Rochester, Minnesota 55905, USA
    Biochemistry 41:11126-33. 2002
    ..The common SCAD polymorphisms may lead to clinically relevant alterations in enzyme function...
  27. ncbi request reprint Mass spectral determination of skeletal/cardiac actin isoform ratios in cardiac muscle
    H Robert Bergen
    W M Keck FT ICR Mass Spectrometry Laboratory, Mayo Proteomics Research Center, Mayo Clinic and Foundation, Rochester, MN 55905, USA
    Rapid Commun Mass Spectrom 17:1467-71. 2003
    ..Additionally, a new dual electrospray ionization source was employed to determine accurate masses of the alpha-skeletal and alpha-cardiac actins...
  28. ncbi request reprint A novel approach to the characterization of substrate specificity in short/branched chain Acyl-CoA dehydrogenase
    Miao He
    Department of Medical Genetics, Mayo Clinic, Rochester, Minnesota 55905, USA
    J Biol Chem 278:37974-86. 2003
    ..Modeling predicts that these residues combine to determine the distance between the flavin ring of FAD and the catalytic base, without changing the opening of the substrate binding pocket...
  29. ncbi request reprint Solution NMR of acetylcholine binding protein reveals agonist-mediated conformational change of the C-loop
    Fan Gao
    Receptor Biology Laboratory, Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine, Rochester MN 55905, USA
    Mol Pharmacol 70:1230-5. 2006
    ..The collective findings suggest a structural mechanism for agonist recognition in AChBP and related Cys-loop receptors...
  30. pmc PDLIM4, an actin binding protein, suppresses prostate cancer cell growth
    Donkena Krishna Vanaja
    Department of Urology, Mayo Clinic College of Medicine, Mayo Clinic, Rochester, MN 55905, USA
    Cancer Invest 27:264-72. 2009
    ..These results suggest that PDLIM4 may function as a tumor suppressor, involved in the control of cell proliferation by associating with actin in prostate cancer cells...
  31. ncbi request reprint Rotation of actin monomers during isometric contraction of skeletal muscle
    Julian Borejdo
    University of North Texas Health Science Center, Department of Molecular Biology and Immunology, Fort Worth, Texas 76107, USA
    J Biomed Opt 12:014013. 2007
    ..The root mean-squared anisotropy fluctuations are greater in isometrically contracting than in rigor myofibrils. The results support the view that during isometric contraction, cross-bridges undergo a mechanical cycle...
  32. pmc Chemical decoupling of ATPase activation and force production from the contractile cycle in myosin by steric hindrance of lever-arm movement
    Andras Muhlrad
    Department of Oral Biology, Hebrew University Hadassah School of Dental Medicine, Jerusalem, Israel 91120
    Biophys J 84:1047-56. 2003
    ..Together these findings suggest that TNP uncouples actin activation of myosin ATPase and the power stroke from other steps in the contraction cycle by inhibiting the converter and lever-arm domain movements...
  33. ncbi request reprint Cosolvent-induced aggregation inhibits myosin ATPase activity by stabilizing the predominant transition intermediate
    Y Michael Peyser
    Department of Oral Biology, Hebrew University Hadassah School of Dental Medicine, Jerusalem, Israel 91120
    Biochemistry 42:12669-75. 2003
    ..This step is presumably inhibited because the coupled swinging of the lever arm from the closed to the open position is constrained by the close packing of aggregated S1...

Research Grants20

  1. In Situ Sensing of Single Myosin Function in Hypertrophy Disease
    Thomas P Burghardt; Fiscal Year: 2010
    ..The proposed research applies promising new computational and experimental approaches for assessing how disease implicated mutations change the motor powering contraction. ..
  2. MOLECULAR ANGLE TRANSITIONS IN MUSCLE
    THOMAS BURGHARDT; Fiscal Year: 2001
    ....
  3. Visualizing Actomyosin Transients by Data Merging
    THOMAS BURGHARDT; Fiscal Year: 2007
    ..The structure to spectrum translation routines have broader implications for structure sensing optical spectroscopy in biophysics. ..
  4. Visualizing Actomyosin Transients by Data Merging
    Thomas P Burghardt; Fiscal Year: 2010
    ..The proposed research promotes understanding of its design for energy conversion shaping approaches for how to repair an ailing motor and how to adapt it to applications where muscle productivity is limiting human potential. ..
  5. MOLECULAR ANGLE TRANSITIONS IN MUSCLE
    THOMAS BURGHARDT; Fiscal Year: 1993
    ..PFPR and TEPR investigate probe movement in the three degrees of rotational freedom. The combination of static and dynamic probe order information provides a comprehensive description of the actomyosin interaction...
  6. MOLECULAR ANGLE TRANSITIONS IN MUSCLE
    THOMAS BURGHARDT; Fiscal Year: 1992
    ..The bleached region is monitored with an attenuated polarized light source and the recovery of fluorescence is observed as unbleached fluorophores rotate and their dipoles move into alignment with the attenuated polarized source...