Shuguang Zhang

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. ncbi request reprint Hierarchical self-assembly of a coiled-coil peptide into fractal structure
    Andrea Lomander
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA
    Nano Lett 5:1255-60. 2005
  2. ncbi request reprint Designer self-assembling peptide scaffolds for 3-d tissue cell cultures and regenerative medicine
    Fabrizio Gelain
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
    Macromol Biosci 7:544-51. 2007
  3. pmc High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81
    Hidehito Takayama
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 3:e2314. 2008
  4. pmc Structure and function analyses of the purified GPCR human vomeronasal type 1 receptor 1
    Karolina Corin
    Center for Biomedical Engineering, NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Sci Rep 1:172. 2011
  5. pmc Significant type I and type III collagen production from human periodontal ligament fibroblasts in 3D peptide scaffolds without extra growth factors
    Yoshiyuki Kumada
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 5:e10305. 2010
  6. pmc Study of two G-protein coupled receptor variants of human trace amine-associated receptor 5
    Xiaoqiang Wang
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Sci Rep 1:102. 2011
  7. pmc Study of a synthetic human olfactory receptor 17-4: expression and purification from an inducible mammalian cell line
    Brian L Cook
    Department of Biological Engineering and Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 3:e2920. 2008
  8. pmc High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1
    Hui Ren
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
    PLoS ONE 4:e4509. 2009
  9. pmc Biological designer self-assembling peptide nanofiber scaffolds significantly enhance osteoblast proliferation, differentiation and 3-D migration
    Akihiro Horii
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 2:e190. 2007
  10. pmc Designer lipid-like peptides: a class of detergents for studying functional olfactory receptors using commercial cell-free systems
    Karolina Corin
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 6:e25067. 2011

Research Grants

  1. Workshop: Self-assembly of Peptides and Proteins
    Shuguang Zhang; Fiscal Year: 2003

Collaborators

Detail Information

Publications75

  1. ncbi request reprint Hierarchical self-assembly of a coiled-coil peptide into fractal structure
    Andrea Lomander
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA
    Nano Lett 5:1255-60. 2005
    ..Such hierarchical organization was modulated by pH or thiol-reducing agent. Exploitation of the fractal structures through chemical methods may be valuable for the fabrication of materials spanning multiple length scales...
  2. ncbi request reprint Designer self-assembling peptide scaffolds for 3-d tissue cell cultures and regenerative medicine
    Fabrizio Gelain
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
    Macromol Biosci 7:544-51. 2007
    ....
  3. pmc High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81
    Hidehito Takayama
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 3:e2314. 2008
    ....
  4. pmc Structure and function analyses of the purified GPCR human vomeronasal type 1 receptor 1
    Karolina Corin
    Center for Biomedical Engineering, NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Sci Rep 1:172. 2011
    ..This expression system can enable structural and functional analyses towards understanding how mammalian scent detection works...
  5. pmc Significant type I and type III collagen production from human periodontal ligament fibroblasts in 3D peptide scaffolds without extra growth factors
    Yoshiyuki Kumada
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 5:e10305. 2010
    ..Our results suggest that these designer self-assembling peptide nanofiber scaffolds may be useful for promoting wound healing and especially periodontal ligament tissue regeneration...
  6. pmc Study of two G-protein coupled receptor variants of human trace amine-associated receptor 5
    Xiaoqiang Wang
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Sci Rep 1:102. 2011
    ..Our study will likely stimulate in the development of therapeutic drug targets for TAAR-associated diseases, as well as fabrication of TAAR-based sensing devices...
  7. pmc Study of a synthetic human olfactory receptor 17-4: expression and purification from an inducible mammalian cell line
    Brian L Cook
    Department of Biological Engineering and Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 3:e2920. 2008
    ..Thus, this system demonstrates the feasibility of purifying milligram quantities of the GPCR membrane protein hOR17-4 for fabrication of olfactory receptor-based bionic sensing device...
  8. pmc High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1
    Hui Ren
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
    PLoS ONE 4:e4509. 2009
    ....
  9. pmc Biological designer self-assembling peptide nanofiber scaffolds significantly enhance osteoblast proliferation, differentiation and 3-D migration
    Akihiro Horii
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 2:e190. 2007
    ..Our results suggest that these designer peptide scaffolds may be very useful for promoting bone tissue regeneration...
  10. pmc Designer lipid-like peptides: a class of detergents for studying functional olfactory receptors using commercial cell-free systems
    Karolina Corin
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 6:e25067. 2011
    ....
  11. pmc A robust and rapid method of producing soluble, stable, and functional G-protein coupled receptors
    Karolina Corin
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 6:e23036. 2011
    ..This robust and simple method may further stimulate others to study the structure and function of membrane proteins...
  12. pmc Structural dynamic of a self-assembling peptide d-EAK16 made of only D-amino acids
    Zhongli Luo
    West China Hospital, Laboratory for Nanobiomedical Technology, Sichuan University, Chengdu, Sichuan, China
    PLoS ONE 3:e2364. 2008
    ..This provides insight into the behaviors of self-assembling peptides made of D-amino acids and points the way to designing new peptide materials for biomedical engineering and nanobiotechnology...
  13. pmc Production of a bioengineered G-protein coupled receptor of human formyl peptide receptor 3
    Xiaoqiang Wang
    Center for Bioengineering and Biotechnology, China University of Petroleum East China, Qingdao, Shandong, China
    PLoS ONE 6:e23076. 2011
    ..Our method can readily produce milligram quantities of human FPR3, which would facilitate in developing human FPR as therapeutic drug targets...
  14. doi request reprint Lipid-like self-assembling peptides
    Shuguang Zhang
    Laboratory of Molecular Design, Center for Bits and Atoms, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139 4307, USA
    Acc Chem Res 45:2142-50. 2012
    ..Likewise, because of their lipophilicity, these structures can deliver molecular cargo, such as small molecules, siRNA, and DNA, in vivo for potential therapeutic applications...
  15. ncbi request reprint Designer self-assembling peptide nanofiber scaffolds for 3D tissue cell cultures
    Shuguang Zhang
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
    Semin Cancer Biol 15:413-20. 2005
    ..The important implications of 3D tissue cell cultures for basic cell biology, tumor biology, high-content drug screening, and regenerative medicine and beyond could be profound...
  16. ncbi request reprint Design of nanostructured biological materials through self-assembly of peptides and proteins
    Shuguang Zhang
    Center for Biomedical Engineering, 56 341 Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Curr Opin Chem Biol 6:865-71. 2002
    ..Peptides and proteins have also been selected to bind metals, semiconductors and ions, inspiring the design of new materials for a wide range of applications in nano-biotechnology...
  17. ncbi request reprint Multi-functional capability of proteins: alpha1-antichymotrypsin and the correlation with Alzheimer's disease
    Shuguang Zhang
    Center for Bioengineering 56 341, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
    J Alzheimers Dis 4:115-22. 2002
  18. pmc Direct conversion of an oligopeptide from a beta-sheet to an alpha-helix: a model for amyloid formation
    S Zhang
    Department of Biology, Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge 02139 4307, USA
    Proc Natl Acad Sci U S A 94:23-8. 1997
    ..Furthermore, such drastic structure changes may also be exploited in biomaterials designed as sensors to detect environmental changes...
  19. ncbi request reprint Fabrication of novel biomaterials through molecular self-assembly
    Shuguang Zhang
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139 4307, USA
    Nat Biotechnol 21:1171-8. 2003
    ....
  20. ncbi request reprint Beyond the Petri dish
    Shuguang Zhang
    Center for Biomedical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139 4307, USA
    Nat Biotechnol 22:151-2. 2004
  21. ncbi request reprint Biological surface engineering: a simple system for cell pattern formation
    S Zhang
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge 02139 4307, USA
    Biomaterials 20:1213-20. 1999
    ..The resulting patterns consist of areas either supporting or inhibiting cell adhesion, thus they are capable of aligning cells in a well-defined manner, leading to specific cell array and pattern formations...
  22. doi request reprint Insertion of T4-lysozyme (T4L) can be a useful tool for studying olfactory-related GPCRs
    Karolina Corin
    Center for Bits and Atoms, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
    Mol Biosyst 8:1750-9. 2012
    ..They also bound their ligands for longer times, suggesting higher receptor stability. Our results indicate that a T4L insertion may be a general method for obtaining GPCRs suitable for structural studies...
  23. ncbi request reprint Systematic studies of a self-assembling peptide nanofiber scaffold with other scaffolds
    Fabrizio Gelain
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
    J Nanosci Nanotechnol 7:424-34. 2007
    ....
  24. pmc Controlled release of functional proteins through designer self-assembling peptide nanofiber hydrogel scaffold
    Sotirios Koutsopoulos
    Center for Biomedical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 106:4623-8. 2009
    ..Our results show that this biocompatible and injectable designer self-assembling peptide hydrogel system may be useful as a carrier for therapeutic proteins for sustained release applications...
  25. pmc Designer short peptide surfactants stabilize G protein-coupled receptor bovine rhodopsin
    Xiaojun Zhao
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
    Proc Natl Acad Sci U S A 103:17707-12. 2006
    ..These peptide surfactants are simple, versatile, effective, and affordable. They represent a designer molecular nanomaterial for use in studies of diverse elusive membrane proteins...
  26. ncbi request reprint Dynamic behaviors of lipid-like self-assembling peptide A6D and A6K nanotubes
    Aki Nagai
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    J Nanosci Nanotechnol 7:2246-52. 2007
    ....
  27. pmc Peptide surfactants for cell-free production of functional G protein-coupled receptors
    Xiaoqiang Wang
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Proc Natl Acad Sci U S A 108:9049-54. 2011
    ..18). These short and simple peptide surfactants may be able to facilitate the rapid production of GPCRs, or even other membrane proteins, for structure and function studies...
  28. pmc Efficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses
    Liselotte Kaiser
    Center for Biomedical Engineering, NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Proc Natl Acad Sci U S A 105:15726-31. 2008
    ..Furthermore, this simple approach may be broadly useful not only for other classes of GPCRs but also for other membrane proteins...
  29. doi request reprint Long-term three-dimensional neural tissue cultures in functionalized self-assembling peptide hydrogels, matrigel and collagen I
    Sotirios Koutsopoulos
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Acta Biomater 9:5162-9. 2013
    ..The well-defined chemical and physical properties of the peptide nanofiber hydrogels and the use of serum-free medium allow for more realistic biological studies of neural cells in a biomimetic 3-D environment...
  30. ncbi request reprint The effect of functionalized self-assembling peptide scaffolds on human aortic endothelial cell function
    Elsa Genove
    Center for Biomedical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Biomaterials 26:3341-51. 2005
    ..These results suggest that this new scaffold provide a better physiological substrate for endothelial cell culture and suggest its further application for biomedical research, cancer biology and regenerative biology...
  31. doi request reprint Designer peptide surfactants stabilize functional photosystem-I membrane complex in aqueous solution for extended time
    Kazuya Matsumoto
    Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA
    J Phys Chem B 113:75-83. 2009
    ..These simple and inexpensive peptide surfactants will likely make significant contributions to stabilize the functional form of diverse and currently elusive membrane proteins and their complexes with important applications...
  32. ncbi request reprint Self-assembly of nanodonut structure from a cone-shaped designer lipid-like peptide surfactant
    Ulung Khoe
    Center for Biomedical Engineering NE47 379, Center for Bits and Atoms, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Langmuir 25:4111-4. 2009
    ..Our observations may be useful for further fine tuning the geometry and shape of a new class of designer peptides and their self-assembled supramolecular materials for diverse uses...
  33. doi request reprint Synergistic effect and hierarchical nanostructure formation in mixing two designer lipid-like peptide surfactants Ac-A6D-OH and Ac-A6K-NH2
    Ulung Khoe
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Macromol Biosci 8:1060-7. 2008
    ..This study will further our understanding for designing new nanomaterials based on designer lipid-like peptide surfactants...
  34. ncbi request reprint Synthesis of monofunctionalized gold nanoparticles by fmoc solid-phase reactions
    Kie Moon Sung
    Massachusetts Institute of Technology, Cambridge, 02139 USA
    J Am Chem Soc 126:5064-5. 2004
    ..By counting the number of 6, we found that at least 60% of cleaved nanoparticles were monofunctionalized by Lys. This method is highly selective and efficient for the preparation of monofunctionalized nanoparticles...
  35. ncbi request reprint Fabrication of molecular materials using peptide construction motifs
    Xiaojun Zhao
    Center for Biomedical Engineering NE47 379, Technology Square 500 and Center for Bits and Atoms, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Trends Biotechnol 22:470-6. 2004
    ..These molecular motifs can now be used to build materials from the bottom up. Biotechnology will continue to harness nature's enormous power to benefit other disciplines and society as a whole...
  36. pmc Optimized expression and purification for high-activity preparations of algal [FeFe]-hydrogenase
    Iftach Yacoby
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 7:e35886. 2012
    ..Low fidelities of maturation result in preparations containing a significant fraction of inactive, apo-protein that are not suitable for biophysical or crystallographic studies...
  37. ncbi request reprint Molecular designer self-assembling peptides
    Xiaojun Zhao
    Institute for Nanobiomedical Technology and Membrane Biology, State Key Lab of Biotherapy of Human Diseases, Cancer Center, West China Hospital, West China Medical School, Sichuan University No 1, Chengdu, Sichuan, China
    Chem Soc Rev 35:1105-10. 2006
    ..Chemistry will again harness Nature's enormous power to benefit other disciplines and society. This tutorial review focuses on two self-assembling peptide systems...
  38. ncbi request reprint Nano hemostat solution: immediate hemostasis at the nanoscale
    Rutledge G Ellis-Behnke
    Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nanomedicine 2:207-15. 2006
    ..Here we report the first use of nanotechnology to achieve complete hemostasis in less than 15 seconds, which could fundamentally change how much blood is needed during surgery of the future...
  39. pmc Dynamic reassembly of peptide RADA16 nanofiber scaffold
    Hidenori Yokoi
    Center for Biomedical Engineering, NE47 379, Massachusetts Institute of Technology, 500 Technology Square, Cambridge, MA 02139 4307, USA
    Proc Natl Acad Sci U S A 102:8414-9. 2005
    ..This reassembly process is important for fabrication of new scaffolds for 3D cell culture, tissue repair, and regenerative medicine...
  40. pmc Protein-mediated error correction for de novo DNA synthesis
    Peter A Carr
    Center for Bits and Atoms, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Nucleic Acids Res 32:e162. 2004
    ..The approach is general, scalable and can be iterated multiple times for greater fidelity. Reductions in both costs and time required are demonstrated for the synthesis of a 2.5 kb gene...
  41. doi request reprint Fabrication of self-assembling D-form peptide nanofiber scaffold d-EAK16 for rapid hemostasis
    Zhongli Luo
    West China Hospital, Laboratory for Nanobiomedical Technology, Sichuan University, Chengdu, Sichuan 610065, China
    Biomaterials 32:2013-20. 2011
    ..We here propose a plausible model not only to provide insights in understanding the chiral assembly properties for rapid hemostasis, but also to aid in further design of self-assembling d-form peptide scaffolds for clinical applications...
  42. pmc Large-scale production and study of a synthetic G protein-coupled receptor: human olfactory receptor 17-4
    Brian L Cook
    Department of Biological Engineering, and Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
    Proc Natl Acad Sci U S A 106:11925-30. 2009
    ..Our system is feasible for the production of large quantities of OR necessary for structural and functional analyses and research into OR biosensor devices...
  43. ncbi request reprint Entrapment of migrating hippocampal neural cells in three-dimensional peptide nanofiber scaffold
    Carlos E Semino
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Tissue Eng 10:643-55. 2004
    ..This simple method may be useful not only in developing technology for neural progenitor cell isolation and enrichment in vitro, but also for expanding cells for cell-based therapies of regenerative medicine...
  44. pmc Kinetic control of dimer structure formation in amyloid fibrillogenesis
    Wonmuk Hwang
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 101:12916-21. 2004
    ..These results suggest that kinetic trapping could play a role in the structural evolution of early aggregates in amyloid fibrillogenesis...
  45. ncbi request reprint Self-organization of a chiral D-EAK16 designer peptide into a 3D nanofiber scaffold
    Zhongli Luo
    West China Hospital, Laboratory for Nanobiomedical Technology, Sichuan University, Chengdu, Sichuan 610065, China
    Macromol Biosci 8:785-91. 2008
    ..We also show that D-peptide nanofibers are resistant to natural proteases and may thus be useful in biotechnology, nanobiotechnology, tissue repair and tissue regeneration as well as other medical applications...
  46. ncbi request reprint Designer self-assembling peptide materials
    Xiaojun Zhao
    Institute for Nanobiomedical Technology and Membrane Biology, West China Hospital, Sichuan University, No 1, Ke Yuan 4th Street, Gao Peng Road, Chengdu, 610041, Sichuan, China
    Macromol Biosci 7:13-22. 2007
    ..image: see text]..
  47. pmc Molecular self-assembly of surfactant-like peptides to form nanotubes and nanovesicles
    Sylvain Vauthey
    Center for Biomedical Engineering, 56 341, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Proc Natl Acad Sci U S A 99:5355-60. 2002
    ..Studies of peptide surfactant molecules have significant implications in the design of nonlipid biological surfactants and the understanding of the complexity and dynamics of the self-assembly processes...
  48. doi request reprint Designer peptide surfactants stabilize diverse functional membrane proteins
    Sotirios Koutsopoulos
    Center for Biomedical Engineering, Massachusetts Institute of Technology, NE47, 500 Technology Square, Cambridge, MA 02139 4307, USA
    Chem Soc Rev 41:1721-8. 2012
    ..We discuss future trends for exploiting spatial features, thermodynamic parameters, and self-assembling properties to create peptide surfactant structures to facilitate the characterization of diverse membrane proteins...
  49. pmc Photosynthetic electron partitioning between [FeFe]-hydrogenase and ferredoxin:NADP+-oxidoreductase (FNR) enzymes in vitro
    Iftach Yacoby
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Proc Natl Acad Sci U S A 108:9396-401. 2011
    ..These results suggest a new direction for improvement of biohydrogen production and a means to further resolve the mechanisms that control partitioning of photosynthetic electron transport...
  50. ncbi request reprint Slow release of molecules in self-assembling peptide nanofiber scaffold
    Yusuke Nagai
    Center for Biomedical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Cambridge, MA 02139 4307, USA
    J Control Release 115:18-25. 2006
    ..These results indicate that release profiles can be tailored through controlling nanofiber-diffusant molecular level interactions...
  51. ncbi request reprint Designer self-assembling Peptide nanofiber scaffolds for study of 3-d cell biology and beyond
    Shuguang Zhang
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139 4307, USA
    Adv Cancer Res 99:335-62. 2008
    ..In doing so, in vitro experimentation can become truly more predictive of in vivo systems...
  52. ncbi request reprint Design peptide scaffolds for regenerative medicine
    Shuguang Zhang
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
    Adv Exp Med Biol 534:147-63. 2003
  53. doi request reprint Expression, purification and functional analysis of an odorant binding protein AaegOBP22 from Aedes aegypti
    Gang Yang
    Institute for Nanobiomedical Technology and Membrane Biology, Sichuan University, Keyuan 4 St, Gaopeng Avenue, Chengdu 610041, China
    Protein Expr Purif 75:165-71. 2011
    ..The protein functions of AaegOBP22 were tested by fluorescent probe 1-NPN binding assays and ligands competitive binding assays. The results show AaegOBP22 proteins have characteristics of selective binding with various ligands...
  54. pmc Self-assembling peptide detergents stabilize isolated photosystem I on a dry surface for an extended time
    Patrick Kiley
    Center for Biomedical Engineering NE47 379, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Biol 3:e230. 2005
    ..These observations suggest that the peptide detergents may effectively stabilize membrane proteins in the solid-state. These designed peptide detergents may facilitate the study of diverse types of membrane proteins...
  55. ncbi request reprint Two-layered injectable self-assembling peptide scaffold hydrogels for long-term sustained release of human antibodies
    Sotirios Koutsopoulos
    Center for Biomedical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    J Control Release 160:451-8. 2012
    ..5%. Our results show that this fully biocompatible and injectable peptide hydrogel system may be used for controlled release applications as a carrier for therapeutic antibodies...
  56. pmc Parallel gene synthesis in a microfluidic device
    David S Kong
    Center for Bits and Atoms, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Nucleic Acids Res 35:e61. 2007
    ..Such volumes are one to two orders of magnitude lower than those utilized in conventional gene synthesis. The identity of all target genes was verified by sequencing, and the resultant error rate was determined to be 1 per 560 bases...
  57. doi request reprint Slow and sustained release of active cytokines from self-assembling peptide scaffolds
    Fabrizio Gelain
    Center for Nanomedicine and Tissue Engineering, Pad Mariani A O Ospedale Niguarda Ca Granda Piazza dell ospedale maggiore 3, Milan 20162, Italy
    J Control Release 145:231-9. 2010
    ..Our results not only provide evidence for long-term molecular release from self-assembling peptide scaffolds but also inspiration for a plethora of slow molecular release strategies for clinical applications...
  58. pmc Nano neuro knitting: peptide nanofiber scaffold for brain repair and axon regeneration with functional return of vision
    Rutledge G Ellis-Behnke
    Department of Brain and Cognitive Science, Massachusetts Institute of Technology, 77 Massachusett Avenue, Cambridge, MA 02139 4307, USA
    Proc Natl Acad Sci U S A 103:5054-9. 2006
    ..The peptide nanofiber scaffold not only represents a previously undiscovered nanobiomedical technology for tissue repair and restoration but also raises the possibility of effective treatment of CNS and other tissue or organ trauma...
  59. ncbi request reprint Functional differentiation of hepatocyte-like spheroid structures from putative liver progenitor cells in three-dimensional peptide scaffolds
    Carlos E Semino
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Differentiation 71:262-70. 2003
    ..This bioengineered scaffold system provides a better physiological approach to "progenitor cell differentiation" for future biomedical and pharmaceutics applications...
  60. doi request reprint A robust, rapid, and simple method of producing olfactory receptors using commercial E. coli cell-free systems
    Karolina Corin
    Center for Bits and Atoms, Massachusetts Institute of Technology, Cambridge, MA, USA
    Methods Mol Biol 1003:101-8. 2013
    ..The receptors can be purified using immunoaffinity chromatography and gel filtration, and can be analyzed using gel electrophoresis and with other standard techniques...
  61. ncbi request reprint Effects of systematic variation of amino acid sequence on the mechanical properties of a self-assembling, oligopeptide biomaterial
    Michael R Caplan
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139, USA
    J Biomater Sci Polym Ed 13:225-36. 2002
    ..Thus, diverse aspects of these molecules can be designed rationally to yield desirable materials properties of different types...
  62. ncbi request reprint More Cinderella than ugly sister
    Shuguang Zhang
    Massachusetts Institute of Technology, Laboratory of Molecular Self Assembly, USA
    Nat Rev Genet 4:243. 2003
  63. pmc Ultra-small-sample molecular structure detection using microslot waveguide nuclear spin resonance
    Yael Maguire
    Center for Bits and Atoms, NE47 379, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
    Proc Natl Acad Sci U S A 104:9198-203. 2007
    ....
  64. pmc Source of sustained voltage difference between the xylem of a potted Ficus benjamina tree and its soil
    Christopher J Love
    Center for Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 3:e2963. 2008
    ..We attribute these sustained voltages to a biological concentration cell likely set up by the homeostatic mechanisms of the tree. Potential applications of this finding are briefly explored...
  65. ncbi request reprint Remote electronic control of DNA hybridization through inductive coupling to an attached metal nanocrystal antenna
    Kimberly Hamad-Schifferli
    The Media Laboratory, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, Massachusetts 02139, USA
    Nature 415:152-5. 2002
    ..Inductive heating of macroscopic samples is widely used, but the present approach should allow extension of this concept to the control of hybridization and thus of a broad range of biological functions on the molecular scale...
  66. ncbi request reprint A classic assembly of nanobiomaterials
    Andreas Mershin
    Nat Biotechnol 23:1379-80. 2005
  67. ncbi request reprint Hydrogels: Wet or let die
    Shuguang Zhang
    Nat Mater 3:7-8. 2004
  68. pmc Injectable self-assembling peptide nanofibers create intramyocardial microenvironments for endothelial cells
    Michael E Davis
    Cardiovascular Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, USA
    Circulation 111:442-50. 2005
    ..We hypothesized that a novel approach to promote vascularization would be to create injectable microenvironments within the myocardium that recruit endothelial cells and promote their survival and organization...
  69. doi request reprint Molecular self-assembly: another brick in the wall
    Shuguang Zhang
    Nat Nanotechnol 1:169-70. 2006
  70. ncbi request reprint Interfacial nano-structuring of designed peptides regulated by solution pH
    Jian R Lu
    Biological Physics Group, Department of Physics, UMIST, PO Box 88, Manchester M60 1QD, United Kingdom
    J Am Chem Soc 126:8940-7. 2004
    ..Subsequent assessment of the reversibility of adsorption showed that once the peptide layers were formed they did not desorb. These interfacial structures may provide feasible routes to interfacial nano-templating...
  71. doi request reprint Incorporation of a matrix metalloproteinase-sensitive substrate into self-assembling peptides - a model for biofunctional scaffolds
    Ying Chau
    Department of Chemical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Hong Kong, China
    Biomaterials 29:1713-9. 2008
    ..The new design can be used for introducing biological functions into self-assembling peptides to create scaffolding materials with potential applications in areas such as tissue engineering and regenerative medicine...
  72. pmc Local myocardial insulin-like growth factor 1 (IGF-1) delivery with biotinylated peptide nanofibers improves cell therapy for myocardial infarction
    Michael E Davis
    Cardiovascular Division, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02139, USA
    Proc Natl Acad Sci U S A 103:8155-60. 2006
    ..Finally, cell therapy with IGF-1 delivery by biotinylated nanofibers improved systolic function after experimental myocardial infarction, demonstrating how engineering the local cellular microenvironment can improve cell therapy...
  73. ncbi request reprint The enhancement of osteoblast growth and differentiation in vitro on a peptide hydrogel-polyHIPE polymer hybrid material
    Maria A Bokhari
    School of Chemical Engineering and Advanced Materials, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK
    Biomaterials 26:5198-208. 2005
    ..We have developed a microcellular polymer containing a nanoscale environment to enhance cell: biomaterial interactions and promote osteoblast growth in vitro...
  74. ncbi request reprint Peptergents: peptide detergents that improve stability and functionality of a membrane protein, glycerol-3-phosphate dehydrogenase
    Joanne I Yeh
    Department of Molecular and Cell Biology, Brown University, Providence, Rhode Island 02912, USA
    Biochemistry 44:16912-9. 2005
    ....

Research Grants1

  1. Workshop: Self-assembly of Peptides and Proteins
    Shuguang Zhang; Fiscal Year: 2003
    ..Hybrid subjects are often astonishingly fertile, whereas if a scientific discipline remains too pure it usually wilts." ..