Michael Yaffe

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. ncbi request reprint Phosphotyrosine-binding domains in signal transduction
    Michael B Yaffe
    Center for Cancer Research, E18 580, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Nat Rev Mol Cell Biol 3:177-86. 2002
  2. pmc 14-3-3 transits to the nucleus and participates in dynamic nucleocytoplasmic transport
    Anne Brunet
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Cell Biol 156:817-28. 2002
  3. pmc The bromodomain protein Brd4 insulates chromatin from DNA damage signalling
    Scott R Floyd
    Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 498:246-50. 2013
  4. pmc A mitotic phosphorylation feedback network connects Cdk1, Plk1, 53BP1, and Chk2 to inactivate the G(2)/M DNA damage checkpoint
    Marcel A T M van Vugt
    David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Biol 8:e1000287. 2010
  5. pmc Combined experimental and computational analysis of DNA damage signaling reveals context-dependent roles for Erk in apoptosis and G1/S arrest after genotoxic stress
    Andrea R Tentner
    Department of Biology, David H Koch Institute for Integrative Cancer Research at MIT, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Syst Biol 8:568. 2012
  6. doi request reprint The scientific drunk and the lamppost: massive sequencing efforts in cancer discovery and treatment
    Michael B Yaffe
    Science Signaling, American Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005, USA
    Sci Signal 6:pe13. 2013
  7. pmc A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1
    Yan Jiang
    Karmanos Cancer Institute, Wayne State University and John D, Dingell VA Medical Center, Room B4325, 4646 John R, Detroit, MI 48201, USA
    J Mol Signal 5:7. 2010
  8. pmc Substrate specificity analysis of protein kinase complex Dbf2-Mob1 by peptide library and proteome array screening
    Angie S Mah
    Department of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    BMC Biochem 6:22. 2005
  9. pmc Signaling netwErks get the global treatment
    Michael B Yaffe
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Genome Biol 8:202. 2007
  10. ncbi request reprint The use of in vitro peptide-library screens in the analysis of phosphoserine/threonine-binding domain structure and function
    Michael B Yaffe
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Annu Rev Biophys Biomol Struct 33:225-44. 2004

Research Grants

  1. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael B Yaffe; Fiscal Year: 2010
  2. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2001
  3. Phosphoserine Dependant Assembly of Signaling complexes
    Michael Yaffe; Fiscal Year: 2006
  4. Protein Kinase Signaling and Cell Cycle Control
    Michael Yaffe; Fiscal Year: 2007
  5. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2007
  6. Phosphoserine Dependant Assembly of Signaling complexes
    Michael Yaffe; Fiscal Year: 2007
  7. Cell Growth & Proliferation Gordon Research Conference
    Michael Yaffe; Fiscal Year: 2007
  8. Protein Kinase Signaling and Cell Cycle Control
    Michael Yaffe; Fiscal Year: 2009
  9. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2009
  10. Protein Kinase Signaling and Cell Cycle Control
    Michael B Yaffe; Fiscal Year: 2010

Detail Information

Publications75

  1. ncbi request reprint Phosphotyrosine-binding domains in signal transduction
    Michael B Yaffe
    Center for Cancer Research, E18 580, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Nat Rev Mol Cell Biol 3:177-86. 2002
    ..Here, the function, structure, and cell biology of phosphotyrosine-binding domains is discussed...
  2. pmc 14-3-3 transits to the nucleus and participates in dynamic nucleocytoplasmic transport
    Anne Brunet
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Cell Biol 156:817-28. 2002
    ..These results indicate that 14-3-3 can mediate the relocalization of nuclear ligands by several mechanisms that ensure complete sequestration of the bound 14-3-3 complex in the cytoplasm...
  3. pmc The bromodomain protein Brd4 insulates chromatin from DNA damage signalling
    Scott R Floyd
    Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 498:246-50. 2013
    ..These data implicate Brd4, previously known for its role in transcriptional control, as an insulator of chromatin that can modulate the signalling response to DNA damage...
  4. pmc A mitotic phosphorylation feedback network connects Cdk1, Plk1, 53BP1, and Chk2 to inactivate the G(2)/M DNA damage checkpoint
    Marcel A T M van Vugt
    David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Biol 8:e1000287. 2010
    ....
  5. pmc Combined experimental and computational analysis of DNA damage signaling reveals context-dependent roles for Erk in apoptosis and G1/S arrest after genotoxic stress
    Andrea R Tentner
    Department of Biology, David H Koch Institute for Integrative Cancer Research at MIT, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Syst Biol 8:568. 2012
    ....
  6. doi request reprint The scientific drunk and the lamppost: massive sequencing efforts in cancer discovery and treatment
    Michael B Yaffe
    Science Signaling, American Association for the Advancement of Science, 1200 New York Avenue NW, Washington, DC 20005, USA
    Sci Signal 6:pe13. 2013
    ....
  7. pmc A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1
    Yan Jiang
    Karmanos Cancer Institute, Wayne State University and John D, Dingell VA Medical Center, Room B4325, 4646 John R, Detroit, MI 48201, USA
    J Mol Signal 5:7. 2010
    ..CARP-1/CCAR1, a perinuclear phospho-protein, regulates signaling by adriamycin, steroids, or growth factors. However, intracellular events that regulate CARP-1-dependent cell growth are not fully understood...
  8. pmc Substrate specificity analysis of protein kinase complex Dbf2-Mob1 by peptide library and proteome array screening
    Angie S Mah
    Department of Biology, California Institute of Technology, Pasadena, CA 91125, USA
    BMC Biochem 6:22. 2005
    ..The components of MEN that act upstream of Dbf2-Mob1 have been characterized, but physiological substrates for Dbf2-Mob1 have yet to be identified...
  9. pmc Signaling netwErks get the global treatment
    Michael B Yaffe
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Genome Biol 8:202. 2007
    ..Two landmark studies of cell signaling, by RNA interference and phosphoproteomics, provide complementary global views of the pathways downstream of receptor kinases, including those regulated by Erks...
  10. ncbi request reprint The use of in vitro peptide-library screens in the analysis of phosphoserine/threonine-binding domain structure and function
    Michael B Yaffe
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Annu Rev Biophys Biomol Struct 33:225-44. 2004
    ..Information emerging from these studies is critical for the design of novel experimental and therapeutic tools aimed at altering signal transduction cascades in normal and diseased cells...
  11. pmc Master of all things phosphorylated
    Michael B Yaffe
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Biochem J 379:e1-2. 2004
    ..This study paves the way for future work on these important 14-3-3-interacting proteins...
  12. ncbi request reprint "Bits" and pieces
    Michael B Yaffe
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02138, USA
    Sci STKE 2006:pe28. 2006
    ..Application of this approach to the genomes of yeast, fruit flies, nematodes, and humans has doubled the number of known or suspected protein-protein interaction motifs...
  13. ncbi request reprint A motif-based profile scanning approach for genome-wide prediction of signaling pathways
    M B Yaffe
    Division of Signal Transduction, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Institutes of Medicine, 10th Floor, 330 Brookline Ave Boston, MA 02215 USA
    Nat Biotechnol 19:348-53. 2001
    ..This technology facilitates prediction of cell signaling networks within proteomes, and could aid in the identification of drug targets for the treatment of human diseases...
  14. ncbi request reprint How do 14-3-3 proteins work?-- Gatekeeper phosphorylation and the molecular anvil hypothesis
    Michael B Yaffe
    Center for Cancer Research E18 580, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    FEBS Lett 513:53-7. 2002
    ..If correct, then 14-3-3 may prove to be a bona fide phosphodependent signaling chaperone...
  15. ncbi request reprint Phosphoserine/threonine-binding domains
    M B Yaffe
    Center for Cancer Research E18 580, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Curr Opin Cell Biol 13:131-8. 2001
    ..The structural basis for pSer/Thr binding differs dramatically between 14-3-3 proteins, WW domains and forkhead-associated domains, suggesting that their pSer/Thr binding function was acquired through convergent evolution...
  16. ncbi request reprint PhosphoSerine/threonine binding domains: you can't pSERious?
    M B Yaffe
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Structure 9:R33-8. 2001
    ..Here, we review the insights into serine/threonine phosphorylation-dependent signal transduction processes provided by structures of several of these proteins and their complexes...
  17. ncbi request reprint The structural basis for 14-3-3:phosphopeptide binding specificity
    M B Yaffe
    Department of Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02215, USA
    Cell 91:961-71. 1997
    ..Finally, we show that the 14-3-3 dimer binds tightly to single molecules containing tandem repeats of phosphoserine motifs, implicating bidentate association as a signaling mechanism with molecules such as Raf, BAD, and Cbl...
  18. ncbi request reprint Insulin receptor substrate proteins create a link between the tyrosine phosphorylation cascade and the Ca2+-ATPases in muscle and heart
    P Algenstaedt
    Research Division, Joslin Diabetes Center and Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    J Biol Chem 272:23696-702. 1997
    ....
  19. ncbi request reprint The PX domains of p47phox and p40phox bind to lipid products of PI(3)K
    F Kanai
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Cell Biol 3:675-8. 2001
    ....
  20. pmc TAZ: a novel transcriptional co-activator regulated by interactions with 14-3-3 and PDZ domain proteins
    F Kanai
    Division of Signal Transduction, Department of Medicine and Department of Surgery, Beth Israel Deaconess Medical Center, Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 19:6778-91. 2000
    ..TAZ may link events at the plasma membrane and cytoskeleton to nuclear transcription in a manner that can be regulated by 14-3-3...
  21. pmc Structural basis for the interaction of the free SH2 domain EAT-2 with SLAM receptors in hematopoietic cells
    M Morra
    Division of Immunology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA
    EMBO J 20:5840-52. 2001
    ..We conclude that EAT-2 plays a role in controlling signal transduction through at least four receptors expressed on the surface of professional antigen-presenting cells...
  22. ncbi request reprint MDC1 directly binds phosphorylated histone H2AX to regulate cellular responses to DNA double-strand breaks
    Manuel Stucki
    The Wellcome Trust Cancer Research UK Gurdon Institute and Department of Zoology, Cambridge University, Tennis Court Road, Cambridge CB2 1QN, United Kingdom
    Cell 123:1213-26. 2005
    ..Thus, binding of MDC1/NFBD1 to gammaH2AX plays a central role in the mammalian response to DNA damage...
  23. ncbi request reprint Molecular basis for the recognition of phosphorylated and phosphoacetylated histone h3 by 14-3-3
    Neil MacDonald
    Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, SK10 4TJ, United Kingdom
    Mol Cell 20:199-211. 2005
    ..14-3-3 isoforms thus represent a class of proteins that mediate the effect of histone phosphorylation at inducible genes...
  24. ncbi request reprint Natural-like function in artificial WW domains
    William P Russ
    Howard Hughes Medical Institute and Department of Pharmacology, University of Texas Southwestern Medical Center, Dallas, Texas 75390 9050, USA
    Nature 437:579-83. 2005
    ..The ability to recapitulate natural-like function in designed sequences shows that a relatively small quantity of sequence information is sufficient to specify the global energetics of amino acid interactions...
  25. ncbi request reprint Polo-like kinase 1 creates the tension-sensing 3F3/2 phosphoepitope and modulates the association of spindle-checkpoint proteins at kinetochores
    Leena J Ahonen
    Molecular, Cell and Developmental Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA
    Curr Biol 15:1078-89. 2005
    ..Determining the kinase that generates the 3F3/2 phosphoepitope at kinetochores should reveal an important component of this system that regulates mitotic progression...
  26. ncbi request reprint Novel at the library
    Michael B Yaffe
    Nat Methods 1:13-4. 2004
  27. ncbi request reprint A structural basis for 14-3-3sigma functional specificity
    Erik W Wilker
    Center for Cancer Research, Department of Biology and Division of Biological Engineering, Massachsuetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 280:18891-8. 2005
    ....
  28. pmc Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis
    Orion D Weiner
    Department of Systems Biology, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Biol 4:e38. 2006
    ....
  29. ncbi request reprint Structural determinants of 14-3-3 binding specificities and regulation of subcellular localization of 14-3-3-ligand complexes: a comparison of the X-ray crystal structures of all human 14-3-3 isoforms
    Alexandra K Gardino
    Center for Cancer Research, Department of Biology and Division of Biomedical Engineering, Massachusetts Institute of Technology, Cambridge, 02139, USA
    Semin Cancer Biol 16:173-82. 2006
    ..Here, we use structural comparisons between isoforms as a framework for discussion of ligand binding by 14-3-3 as well as the mechanisms through which post-translational modification of the different isoforms alters their function...
  30. ncbi request reprint Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis
    Satoshi Yoshida
    Department of Pediatric Oncology, Dana Farber Cancer Institute and Division of Hematology Oncology, Children s Hospital Boston and Harvard Medical School, Boston, MA 02115, USA
    Science 313:108-11. 2006
    ..This role of Cdc5 (Polo-like kinase) in regulating Rho1 is likely to be relevant to cytokinesis and asymmetric cell division in other organisms...
  31. ncbi request reprint Linear motif atlas for phosphorylation-dependent signaling
    Martin Lee Miller
    Center for Biological Sequence Analysis, Technical University of Denmark, 2800 Lyngby, Denmark
    Sci Signal 1:ra2. 2008
    ..The atlas is available as a community resource (http://netphorest.info)...
  32. pmc Fc gamma R-stimulated activation of the NADPH oxidase: phosphoinositide-binding protein p40phox regulates NADPH oxidase activity after enzyme assembly on the phagosome
    Wei Tian
    Herman B Wells Center for Pediatric Research, Department of Pediatrics Hematology Oncology, Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    Blood 112:3867-77. 2008
    ..We conclude that p40(phox) functions primarily to regulate FcgammaR-induced NADPH oxidase activity rather than assembly, and stimulates superoxide production via a PI3P signal that increases after phagosome internalization...
  33. ncbi request reprint Polo-like kinase-1 is activated by aurora A to promote checkpoint recovery
    Libor Macurek
    Department of Medical Oncology, University Medical Center Utrecht, Utrecht 3584CG, The Netherlands
    Nature 455:119-23. 2008
    ..Taken together, these data demonstrate that the initial activation of PLK1 is a primary function of aurora A...
  34. pmc The phosphoinositide-binding protein p40phox activates the NADPH oxidase during FcgammaIIA receptor-induced phagocytosis
    Chang Il Suh
    Department of Pediatrics Hematology Oncology, Herman B Wells Center for Pediatric Research, Riley Hospital for Children, Indiana University School of Medicine, Indianapolis, IN 46202, USA
    J Exp Med 203:1915-25. 2006
    ..Hence, this study identifies a role for p40(phox) and PI(3)P in coupling FcgammaR-mediated phagocytosis to activation of the NADPH oxidase...
  35. pmc RNF8 transduces the DNA-damage signal via histone ubiquitylation and checkpoint protein assembly
    Michael S Y Huen
    Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, CT 06520, USA
    Cell 131:901-14. 2007
    ..Together, our study implicates RNF8 as a novel DNA-damage-responsive protein that integrates protein phosphorylation and ubiquitylation signaling and plays a critical role in the cellular response to genotoxic stress...
  36. pmc NetworKIN: a resource for exploring cellular phosphorylation networks
    Rune Linding
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nucleic Acids Res 36:D695-9. 2008
    ..The database currently contains a predicted phosphorylation network with 20,224 site-specific interactions involving 3978 phosphoproteins and 73 human kinases from 20 families...
  37. pmc Systematic discovery of in vivo phosphorylation networks
    Rune Linding
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Cell 129:1415-26. 2007
    ..Applying this approach to DNA damage signaling, we show that 53BP1 and Rad50 are phosphorylated by CDK1 and ATM, respectively. We describe a scalable strategy to evaluate predictions, which suggests that BCLAF1 is a GSK-3 substrate...
  38. pmc Proteomic screen defines the Polo-box domain interactome and identifies Rock2 as a Plk1 substrate
    Drew M Lowery
    Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    EMBO J 26:2262-73. 2007
    ....
  39. ncbi request reprint ROS fusion tyrosine kinase activates a SH2 domain-containing phosphatase-2/phosphatidylinositol 3-kinase/mammalian target of rapamycin signaling axis to form glioblastoma in mice
    Al Charest
    Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Cancer Res 66:7473-81. 2006
    ..These findings provide novel and important insights into the role of ROS and SHP-2 function in solid tumor biology and set the stage for preclinical testing of targeted therapeutic approaches...
  40. ncbi request reprint Structure and function of Polo-like kinases
    Drew M Lowery
    Center for Cancer Research, E18 580, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Oncogene 24:248-59. 2005
    ..Molecular modelling of the structure of the isolated kinase domain reveals a potential basis for motif-dependent substrate specificity...
  41. ncbi request reprint MAPKAP kinase-2 is a cell cycle checkpoint kinase that regulates the G2/M transition and S phase progression in response to UV irradiation
    Isaac A Manke
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Mol Cell 17:37-48. 2005
    ..We propose that MAPKAP kinase-2 is a new member of the DNA damage checkpoint kinase family that functions in parallel with Chk1 and Chk2 to integrate DNA damage signaling responses and cell cycle arrest in mammalian cells...
  42. pmc Scansite 2.0: Proteome-wide prediction of cell signaling interactions using short sequence motifs
    John C Obenauer
    Center for Cancer Research, E18 580, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Nucleic Acids Res 31:3635-41. 2003
    ..In addition, a new series of Sequence Match programs for non-quantitative user-defined motifs has been implemented. Scansite is available via the World Wide Web at http://scansite.mit.edu...
  43. ncbi request reprint Study of substrate specificity of MAPKs using oriented peptide libraries
    Michael B Yaffe
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA
    Methods Mol Biol 250:237-50. 2004
  44. ncbi request reprint The Polo-box domain: a molecular integrator of mitotic kinase cascades and Polo-like kinase function
    Drew M Lowery
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Cell Cycle 3:128-31. 2004
  45. ncbi request reprint Signal transduction: molecular monogamy
    Drew Endy
    Nature 426:614-5. 2003
  46. ncbi request reprint Merlin, the product of the Nf2 tumor suppressor gene, is an inhibitor of the p21-activated kinase, Pak1
    Joseph L Kissil
    Department of Biology and Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 12:841-9. 2003
    ..This link provides a possible mechanism for the effect of loss of merlin expression in tumorigenesis...
  47. ncbi request reprint BRCT repeats as phosphopeptide-binding modules involved in protein targeting
    Isaac A Manke
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 302:636-9. 2003
    ....
  48. ncbi request reprint The molecular basis for phosphodependent substrate targeting and regulation of Plks by the Polo-box domain
    Andrew E H Elia
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 115:83-95. 2003
    ..In addition, phosphopeptide binding to the PBD stimulates kinase activity in full-length Plk1, suggesting a conformational switching mechanism for Plk regulation and a dual functionality for the PBD...
  49. ncbi request reprint Proteomic screen finds pSer/pThr-binding domain localizing Plk1 to mitotic substrates
    Andrew E H Elia
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 299:1228-31. 2003
    ..This finding reveals how Plk1 can localize to specific sites within cells in response to Cdk phosphorylation at those sites and provides a structural mechanism for targeting the Plk1 kinase domain to its substrates...
  50. ncbi request reprint Fluorescent caged phosphoserine peptides as probes to investigate phosphorylation-dependent protein associations
    M Eugenio Vazquez
    Department of Chemistry and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 125:10150-1. 2003
    ..At the same time, the newly developed photolabile 1-(2-nitrophenyl)ethyl-caged phosphoserine allows control of the release of the biologically active ligand through unmasking of the key phosphoserine functionality upon UV irradiation...
  51. pmc Proteomic identification of 14-3-3zeta as a mitogen-activated protein kinase-activated protein kinase 2 substrate: role in dimer formation and ligand binding
    David W Powell
    Departments of Biochemistry and Molecular Biology, University of Louisville, Louisville, KY 40202, USA
    Mol Cell Biol 23:5376-87. 2003
    ..These data suggest that MAPKAPK2-mediated phosphorylation regulates 14-3-3zeta functions, and this MAPKAPK2 activity may represent a novel pathway mediating p38 MAPK-dependent inflammation...
  52. ncbi request reprint TAZ interacts with TTF-1 and regulates expression of surfactant protein-C
    Kwon Sik Park
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 279:17384-90. 2004
    ..TAZ binds to TTF-1, increasing the transcriptional activity of TTF-1 on the SP-C promoter. Developmental and cell-selective regulation of TAZ provides a mechanism by which the activity of TTF-1 on target genes is modulated...
  53. ncbi request reprint Computational prediction of protein-protein interactions
    John C Obenauer
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA
    Methods Mol Biol 261:445-68. 2004
    ..This chapter describes in detail how to use Scansite to predict the binding partners of an input protein, and how to find all proteins that contain a given sequence motif...
  54. ncbi request reprint Distinct ligand-dependent roles for p38 MAPK in priming and activation of the neutrophil NADPH oxidase
    Glenn E Brown
    Department of Surgery, Beth Israel Deaconess Medical Center, Boston, Massachusetts 02130, USA
    J Biol Chem 279:27059-68. 2004
    ....
  55. ncbi request reprint 14-3-3 family members act coordinately to regulate mitotic progression
    Sorab N Dalal
    Department of Adult Oncology, Dana Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts 02115, USA
    Cell Cycle 3:672-7. 2004
    ..Thus, specific members of the 14-3-3 family of proteins may act coordinately to maintain the DNA replication checkpoint by regulating the activity of different cell cycle proteins...
  56. ncbi request reprint Structural and functional versatility of the FHA domain in DNA-damage signaling by the tumor suppressor kinase Chk2
    Jiejin Li
    Division of Protein Structure, National Institute for Medical Research, The Ridgeway, Mill Hill, London, United Kingdom
    Mol Cell 9:1045-54. 2002
    ..Although phospho-dependent binding is important for Chk2 activity, previously uncharacterized phospho-independent FHA domain interactions appear to be the primary target of oncogenic lesions...
  57. ncbi request reprint Lamellipodin, an Ena/VASP ligand, is implicated in the regulation of lamellipodial dynamics
    Matthias Krause
    Department of Biology and Center for Cancer Research, MIT, Cambridge, MA 02139, USA
    Dev Cell 7:571-83. 2004
    ..These phenotypes are more severe than loss of Ena/VASP, suggesting that Lpd regulates other effectors of the actin cytoskeleton in addition to Ena/VASP...
  58. ncbi request reprint Cytoplasmic localization of tristetraprolin involves 14-3-3-dependent and -independent mechanisms
    Barbra A Johnson
    Center for Blood Research and Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 277:18029-36. 2002
    ..They also suggest that 14-3-3 binding is part of a complex network of stimuli and interactions that regulate TTP function...
  59. ncbi request reprint 14-3-3 Proteins--a focus on cancer and human disease
    Erik Wilker
    Center for Cancer Research, Massachusetts Institute of Technology, E18 580, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    J Mol Cell Cardiol 37:633-42. 2004
    ....
  60. pmc Phosphatidylinositol 3-phosphate-dependent and -independent functions of p40phox in activation of the neutrophil NADPH oxidase
    Sarah A Bissonnette
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 283:2108-19. 2008
    ..We present a model where p40(phox) translocates p67(phox) to the region of the cytochrome and subsequently switches the oxidase to an activated state dependent upon PtdIns(3)P and SH3 domain engagement...
  61. ncbi request reprint MAGUK SH3 domains--swapped and stranded by their kinases?
    Michael B Yaffe
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Structure 10:3-5. 2002
    ..Just such a series of insights is now beginning to emerge from two recently published structures of the scaffolding protein PSD-95...
  62. ncbi request reprint Proteomics and systems biology approaches to signal transduction in sepsis
    Anhco Nguyen
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Crit Care Med 31:S1-6. 2003
    ..These types of systems biology-based approaches may lead to more effective therapies than those currently available...
  63. ncbi request reprint The p47phox PX domain: two heads are better than one!
    Michael B Yaffe
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Structure 10:1288-90. 2002
    ..This unusual feature allows the p47phox PX domain to integrate signal transduction events emerging from two different lipid signaling pathways...
  64. ncbi request reprint A novel assay system implicates PtdIns(3,4)P(2), PtdIns(3)P, and PKC delta in intracellular production of reactive oxygen species by the NADPH oxidase
    Glenn E Brown
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Mol Cell 11:35-47. 2003
    ..This system should be of great utility for the study of cell signaling events that regulate the intracellular production of reactive oxygen species by neutrophils...
  65. ncbi request reprint SIRT1 shows no substrate specificity in vitro
    Gil Blander
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 280:9780-5. 2005
    ..This result brings us one step closer to understanding how SIRT1 and possibly other protein deacetylases chose their substrate...
  66. ncbi request reprint Chk'n out in mitosis
    Isaac A Manke
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue E18 580, Cambridge, MA 02139, USA
    Cell Cycle 2:236-7. 2003
  67. ncbi request reprint Studying the optimal peptide substrate motifs of protein kinase C using oriented peptide libraries
    Michael B Yaffe
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA
    Methods Mol Biol 233:273-85. 2003
  68. pmc p53-deficient cells rely on ATM- and ATR-mediated checkpoint signaling through the p38MAPK/MK2 pathway for survival after DNA damage
    H Christian Reinhardt
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Cancer Cell 11:175-89. 2007
    ..We show that the Chk1 inhibitor UCN-01 also potently inhibits MK2, suggesting that its clinical efficacy results from the simultaneous disruption of two critical checkpoint pathways in p53-defective cells...
  69. ncbi request reprint A multivalent peptide library approach identifies a novel Shiga toxin inhibitor that induces aberrant cellular transport of the toxin
    Kiyotaka Nishikawa
    Department of Clinical Pharmacology, Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku ku, Tokyo 162 8655, Japan
    FASEB J 20:2597-9. 2006
    ..Thus, the multivalent peptide library approach enabled the identification of a peptide-based Stx2 inhibitor that has remarkable therapeutic potency and appears to function by inducing aberrant cellular transport and degradation of Stx2...
  70. ncbi request reprint X-ray crystallography and structural biology
    Michael B Yaffe
    Department of Biology, Center for Cancer Research, Cambridge, MA, USA
    Crit Care Med 33:S435-40. 2005
  71. ncbi request reprint Structure and mechanism of BRCA1 BRCT domain recognition of phosphorylated BACH1 with implications for cancer
    Julie A Clapperton
    National Institute for Medical Research, Division of Protein Structure, The Ridgeway, Mill Hill, London NW7 1AA, UK
    Nat Struct Mol Biol 11:512-8. 2004
    ..We show that a subset of disease-linked mutations act through specific disruption of phospho-dependent BRCA1 interactions rather than through gross structural perturbation of the tandem BRCT domains...
  72. ncbi request reprint TAZ, a transcriptional modulator of mesenchymal stem cell differentiation
    Jeong Ho Hong
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Science 309:1074-8. 2005
    ..These results indicate that TAZ functions as a molecular rheostat that modulates MSC differentiation...
  73. ncbi request reprint Caged phosphopeptides reveal a temporal role for 14-3-3 in G1 arrest and S-phase checkpoint function
    Anhco Nguyen
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Biotechnol 22:993-1000. 2004
    ..This class of reagents will greatly facilitate molecular dissection of kinase-dependent signaling pathways when applied to other phosphopeptide-binding domains including SH2, Polo-box and tandem BRCT domains...
  74. pmc A computational method for the analysis and prediction of protein:phosphopeptide-binding sites
    Brian A Joughin
    Department of Biology, Center for Cancer Research, Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Protein Sci 14:131-9. 2005
    ..The locations of phosphoresidue contact sites were then predicted on the surfaces of the checkpoint kinase Chk1 and the BRCA1 BRCT repeat domain, and these predictions are consistent with recent experimental evidence...
  75. pmc A coupled chemical-genetic and bioinformatic approach to Polo-like kinase pathway exploration
    Jennifer L Snead
    Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA 94158, USA
    Chem Biol 14:1261-72. 2007
    ..Finally, an examination of Cdc5 binding by SPB-localized proteins expanded our knowledge of Cdc5 function at the SPB...

Research Grants31

  1. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael B Yaffe; Fiscal Year: 2010
    ....
  2. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2001
    ..The results from these studies may assist in the development of novel therapies aimed at limiting the auto-inflammatory tissue damage patients suffer as a result of sepsis and trauma. ..
  3. Phosphoserine Dependant Assembly of Signaling complexes
    Michael Yaffe; Fiscal Year: 2006
    ....
  4. Protein Kinase Signaling and Cell Cycle Control
    Michael Yaffe; Fiscal Year: 2007
    ....
  5. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2007
    ..The results from these studies may assist in the development of novel diagnostic or therapeutic reagents aimed at limiting the auto-imflammatory tissue damage patients suffer as a result of sepsis and trauma. ..
  6. Phosphoserine Dependant Assembly of Signaling complexes
    Michael Yaffe; Fiscal Year: 2007
    ....
  7. Cell Growth & Proliferation Gordon Research Conference
    Michael Yaffe; Fiscal Year: 2007
    ..The Gordon Conference format provides a unique environment that encourages informal and open discussion among the participants and this in turn fosters initiation of collaborative efforts and stimulates future research directions. ..
  8. Protein Kinase Signaling and Cell Cycle Control
    Michael Yaffe; Fiscal Year: 2009
    ..abstract_text> ..
  9. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2009
    ....
  10. Protein Kinase Signaling and Cell Cycle Control
    Michael B Yaffe; Fiscal Year: 2010
    ..abstract_text> ..
  11. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2009
    ....
  12. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2006
    ..The results from these studies may assist in the development of novel diagnostic or therapeutic reagents aimed at limiting the auto-imflammatory tissue damage patients suffer as a result of sepsis and trauma. ..
  13. Phosphoserine Dependant Assembly of Signaling complexes
    Michael Yaffe; Fiscal Year: 2005
    ....
  14. 2004 Growth Factor Signalling Gordon Conference
    Michael Yaffe; Fiscal Year: 2006
    ..abstract_text> ..
  15. PHOSPHOSERINE DEPENDENT ASSEMBLY OF SIGNALING COMPLEXES
    Michael Yaffe; Fiscal Year: 2000
    ..These studies fit within the Applicant's long term objectives to understand the cellular, biochemical, and structural basis for phosphoserine dependent signaling. ..
  16. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2000
    ..The results from these studies may assist in the development of novel therapies aimed at limiting the auto-inflammatory tissue damage patients suffer as a result of sepsis and trauma. ..
  17. PHOSPHOSERINE DEPENDENT ASSEMBLY OF SIGNALING COMPLEXES
    Michael Yaffe; Fiscal Year: 2001
    ..These studies fit within the Applicant's long term objectives to understand the cellular, biochemical, and structural basis for phosphoserine dependent signaling. ..
  18. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2002
    ..The results from these studies may assist in the development of novel therapies aimed at limiting the auto-inflammatory tissue damage patients suffer as a result of sepsis and trauma. ..
  19. PHOSPHOSERINE DEPENDENT ASSEMBLY OF SIGNALING COMPLEXES
    Michael Yaffe; Fiscal Year: 2002
    ..These studies fit within the Applicant's long term objectives to understand the cellular, biochemical, and structural basis for phosphoserine dependent signaling. ..
  20. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2003
    ..The results from these studies may assist in the development of novel therapies aimed at limiting the auto-inflammatory tissue damage patients suffer as a result of sepsis and trauma. ..
  21. PHOSPHOSERINE DEPENDENT ASSEMBLY OF SIGNALING COMPLEXES
    Michael Yaffe; Fiscal Year: 2003
    ..These studies fit within the Applicant's long term objectives to understand the cellular, biochemical, and structural basis for phosphoserine dependent signaling. ..
  22. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2004
    ..The results from these studies may assist in the development of novel diagnostic or therapeutic reagents aimed at limiting the auto-imflammatory tissue damage patients suffer as a result of sepsis and trauma. ..
  23. Phosphoserine Dependant Assembly of Signaling complexes
    Michael Yaffe; Fiscal Year: 2004
    ....
  24. NCRR Shared Instrumentation Grant (SIG) Program
    Michael Yaffe; Fiscal Year: 2005
    ..abstract_text> ..
  25. High-Throughput Assay Design:Polo Kinase Inhibitors(RMI)
    Michael Yaffe; Fiscal Year: 2004
    ..The development of these assays should allow rapid screening of small molecule Polo kinase inhibitors that will function as novel anti-cancer agents. ..
  26. NEUTROPHIL PRIMING IN TRAUMA AND SEPSIS
    Michael Yaffe; Fiscal Year: 2005
    ..The results from these studies may assist in the development of novel diagnostic or therapeutic reagents aimed at limiting the auto-imflammatory tissue damage patients suffer as a result of sepsis and trauma. ..
  27. Identification of Molecular Pathways in Cancer Biology
    Michael Yaffe; Fiscal Year: 2005
    ..abstract_text> ..
  28. Protein Kinase Signaling and Cell Cycle Control
    Michael B Yaffe; Fiscal Year: 2011
    ..abstract_text> ..