Research Topics
Species | M T WharySummaryAffiliation: Massachusetts Institute of Technology Country: USA Publications
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Publications
Verifying and quantifying Helicobacter pylori infection status of research miceMark T Whary
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA
Methods Mol Biol 921:143-56. 2012..pylori rRNA. In this chapter, we will summarize strategies to allow initiation of experiments with helicobacter-free mice and then focus on PCR and ELISA techniques to verify and quantify H. pylori infection of research mice...
Detection, eradication, and research implications of Helicobacter infections in laboratory rodentsMark T Whary
Division of Comparative Medicine, Massachusetts Institute of Technology, 77 Massachusetts Ave, Bldg 16 825A, Cambridge, MA 02139, USA
Lab Anim (NY) 35:25-7, 30-6. 2006..The authors discuss the impact of subclinical and clinical disease and offer recommendations for managing helicobacter-free rodent colonies...- Rapid onset of ulcerative typhlocolitis in B6.129P2-IL10tm1Cgn (IL-10-/-) mice infected with Helicobacter trogontum is associated with decreased colonization by altered Schaedler's floraM T Whary
Division of Comparative Medicine, Massachusette Institute of Technology, 77 Massachusetts Avenue, Bldg 16 825A, Cambridge, MA 02139, USA
Infect Immun 74:6615-23. 2006..trogontum (with or without H. rodentium) induces a rapid-onset, erosive to ulcerative typhlocolitis which impacts the normal anaerobic flora of the colon and increases the risk of sepsis... - Intestinal helminthiasis in Colombian children promotes a Th2 response to Helicobacter pylori: possible implications for gastric carcinogenesisMark T Whary
Division of Comparative Medicine, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Building 16 825A, Cambridge, Massachusetts 02139, USA
Cancer Epidemiol Biomarkers Prev 14:1464-9. 2005..Considering the risk for H. pylori disease seems affected by features of bacterial virulence and host polymorphisms, other poorly understood influences, such as concurrent helminthiasis, may also be important... - Lactobacillus reuteri promotes Helicobacter hepaticus-associated typhlocolitis in gnotobiotic B6.129P2-IL-10(tm1Cgn) (IL-10(-/-) ) miceMark T Whary
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA, USA
Immunology 133:165-78. 2011..reuteri. Although the effects other microbiota may have on H. hepaticus virulence properties remain speculative, further investigations using this gnotobiotic model are now possible... - Chronic active hepatitis induced by Helicobacter hepaticus in the A/JCr mouse is associated with a Th1 cell-mediated immune responseM T Whary
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Infect Immun 66:3142-8. 1998..i. The characterization of a cell-mediated Th1 immune response to H. hepaticus infection in the A/JCr mouse should prove valuable as a model for experimental regimens which manipulate the host response to Helicobacter... - Th1-mediated pathology in mouse models of human disease is ameliorated by concurrent Th2 responses to parasite antigensMark T Whary
Division of Comparative Medicine, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Building 16 825A, Cambridge, MA 02139, USA
Curr Top Med Chem 4:531-8. 2004.... - Cholangiohepatitis and inflammatory bowel disease induced by a novel urease-negative Helicobacter species in A/J and Tac:ICR:HascidfRF miceN H Shomer
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge 02139, USA
Exp Biol Med (Maywood) 226:420-8. 2001..These results provide a new model of IBD and cholangio-hepatitis associated with a specific urease-negative, novel H. species... - Experimental Helicobacter pylori infection induces antral gastritis and gastric mucosa-associated lymphoid tissue in guinea pigsN H Shomer
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Infect Immun 66:2614-8. 1998..pylori-infected humans. The H. pylori-infected guinea pig should prove useful in modeling the interaction of helicobacter and vitamin C in gastric carcinogenesis... - Cytolethal distending toxin promotes Helicobacter cinaedi-associated typhlocolitis in interleukin-10-deficient miceZ Shen
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Infect Immun 77:2508-16. 2009..These results indicate that WT(Hc) can be used to model inflammatory bowel disease in IL-10(-/-) mice and that CDT contributes to the virulence of H. cinaedi... - Fluorogenic PCR-based quantitative detection of a murine pathogen, Helicobacter hepaticusZ Ge
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
J Clin Microbiol 39:2598-602. 2001..hepaticus DNA. Therefore, this assay can be used with high sensitivity and specificity to quantify H. hepaticus in experimentally infected mouse models as well as in naturally infected mice... - Concurrent enteric helminth infection modulates inflammation and gastric immune responses and reduces helicobacter-induced gastric atrophyJ G Fox
Division of Comparative Medicine, Massachusetts Institute of Technology Cambridge, Massachusetts 02139, USA
Nat Med 6:536-42. 2000..Thus, concurrent enteric helminth infection can attenuate gastric atrophy, a premalignant lesion... - Long-term colonization levels of Helicobacter hepaticus in the cecum of hepatitis-prone A/JCr mice are significantly lower than those in hepatitis-resistant C57BL/6 miceM T Whary
Massachusetts Institute of Technology, Cambridge 02139, USA
Comp Med 51:413-7. 2001..hepaticus in the cecum, compared with those of hepatitis-resistant C57BL/6 mice. Host responses of A/JCr mice that limit cecal colonization with H. hepaticus may have important roles in the pathogenesis of hepatic lesions... - Mice carrying a truncated Apc gene have diminished gastric epithelial proliferation, gastric inflammation, and humoral immunity in response to Helicobacter felis infectionJ G Fox
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge 02138, USA
Cancer Res 57:3972-8. 1997.... 
Helicobacter hepaticus infection in mice: models for understanding lower bowel inflammation and cancerJ G Fox
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Mucosal Immunol 4:22-30. 2011..hepaticus pathogenesis. Delineating targets of H. hepaticus virulence factors could facilitate novel approaches to treating microbially induced lower bowel inflammatory diseases...- Helicobacter bilis-associated hepatitis in outbred miceJames G Fox
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Comp Med 54:571-7. 2004..bilis-infected mice of group III seroconverted to H. bilis outer membrane antigen. Helicobacter bilis infection is associated with hepatitis in SW mice and can confound experimental results... - Protective role of 17 beta -estradiol against the development of Helicobacter pylori-induced gastric cancer in INS-GAS miceMasahiro Ohtani
Division of Comparative Medicine, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Building 16 825C, Cambridge, MA 02139, USA
Carcinogenesis 28:2597-604. 2007..These results demonstrate a protective effect of E2 in H.pylori-induced gastric cancer in a mouse model... - Accelerated progression of gastritis to dysplasia in the pyloric antrum of TFF2 -/- C57BL6 x Sv129 Helicobacter pylori-infected miceJames G Fox
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Am J Pathol 171:1520-8. 2007..pylori-infected mice at 19 months pi. These data support a protective role for TFF2 in part by modulating levels of gastric interferon-gamma in the development of H. pylori-associated premalignancy of the distal stomach... - Vitamin C supplementation does not protect L-gulono-gamma-lactone oxidase-deficient mice from Helicobacter pylori-induced gastritis and gastric premalignancyChung Wei Lee
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Int J Cancer 122:1068-76. 2008..pylori-induced premalignant gastric lesions. In addition, less severe gastric lesions in H.pylori infected gulo(-/-) mice supplemented with low vitamin C correlated with an attenuated Th1 inflammatory response... - Helicobacter hepaticus urease is not required for intestinal colonization but promotes hepatic inflammation in male A/JCr miceZhongming Ge
Division of Comparative Medicine, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Microb Pathog 45:18-24. 2008..These results indicate that H. hepaticus urease activity plays a crucial role in hepatic disease but is not required for cecal colonization by H. hepaticus... - Helicobacter hepaticus HHGI1 is a pathogenicity island associated with typhlocolitis in B6.129-IL10 tm1Cgn miceZhongming Ge
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Microbes Infect 10:726-33. 2008..05) at 13 WPI. These results demonstrate that genes in the HHGI1 contribute to the pathogenicity of H. hepaticus, at least in part via up-regulation of proinflammatory mediators IFN-gamma, TNF-alpha and IL-17a... - Helicobacter pylori-associated gastric cancer in INS-GAS mice is gender specificJames G Fox
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Cancer Res 63:942-50. 2003..This study highlights the importance of using both genders to investigate the pathogenesis of H. pylori... - Germ-line p53-targeted disruption inhibits helicobacter-induced premalignant lesions and invasive gastric carcinoma through down-regulation of Th1 proinflammatory responsesJames G Fox
Division of Comparative Medicine, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Cancer Res 62:696-702. 2002.... - Wild-type and interleukin-10-deficient regulatory T cells reduce effector T-cell-mediated gastroduodenitis in Rag2-/- mice, but only wild-type regulatory T cells suppress Helicobacter pylori gastritisChung Wei Lee
Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Infect Immun 75:2699-707. 2007..pylori-mediated inflammation and in regulatory effects between wt T(R) and IL-10(-/-) T(R) cells suggest that IL-10 expression by wt T(R) cells is important to regulatory suppression of gastric inflammation... - Trefoil family factor 2 is expressed in murine gastric and immune cells and controls both gastrointestinal inflammation and systemic immune responsesEvelyn A Kurt-Jones
Department of Medicine, University of Massachusetts Medical School, 364 Plantation Street, Lazare Research Building Rm 226, Worcester, MA 01605, USA
Infect Immun 75:471-80. 2007..Our studies suggest that TFF2 not only controls gastrointestinal repair but also regulates mononuclear cell inflammatory responses... - Gastric helicobacter infection induces a Th2 phenotype but does not elevate serum cholesterol in mice lacking inducible nitric oxide synthaseMelanie Ihrig
Division of Comparative Medicine, Massachusetts Institute of Technology, 77 Massachusetts Ave, Bldg 16, Rm 825C, Cambridge, MA 02139, USA
Infect Immun 73:1664-70. 2005..H. felis colonization was significantly greater in the iNOS-deficient mice. These findings are indicative of an impaired Th1 component of the H. felis-induced inflammatory response when the influence of iNOS is removed... - Cytolethal distending toxin is essential for Helicobacter hepaticus colonization in outbred Swiss Webster miceZhongming Ge
Division of Comparative Medicine, Massachusetts Institute of Technology, 16 873, 77 Massachusetts Avenue, Cambridge, MA 02131, USA
Infect Immun 73:3559-67. 2005..hepaticus in cecum, colon, and jejunum but only transient colonization of H. hepaticus in the ileum of female mice; (iv) H. hepaticus colonization was associated with down-regulation of colonic IL-10 production... - Progression of chronic hepatitis and preneoplasia in Helicobacter hepaticus-infected A/JCr miceArlin B Rogers
Comparative Pathology Laboratory 16 849, Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Toxicol Pathol 32:668-77. 2004..Continued study of chronic hepatitis progression may reveal therapeutic targets to reduce the risk of hepatocellular carcinoma... - Natural and experimental Helicobacter infectionsMark T Whary
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Comp Med 54:128-58. 2004..Current recommendations for deriving and managing helicobacter-free animal colonies for research are provided... - p27kip1 deficiency confers susceptibility to gastric carcinogenesis in Helicobacter pylori-infected miceNoriyoshi Kuzushita
Division of Gastroenterology, Department of Medicine, Rhode Island Hospital Brown University, Providence, Rhode Island 02903, USA
Gastroenterology 129:1544-56. 2005..We therefore evaluated p27-deficient mice as a model for H pylori-associated gastric cancer... - Helicobacter pylori but not high salt induces gastric intraepithelial neoplasia in B6129 miceArlin B Rogers
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Cancer Res 65:10709-15. 2005..In summary, we report for the first time H. pylori-induced gastric intraepithelial neoplasia in a wild-type mouse model and show no additive effect of high-salt ingestion on tumor progression... - Gastroenteritis in NF-kappaB-deficient mice is produced with wild-type Camplyobacter jejuni but not with C. jejuni lacking cytolethal distending toxin despite persistent colonization with both strainsJames G Fox
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Infect Immun 72:1116-25. 2004..jejuni to escape immune surveillance. NF-kappaB-deficient mice should be a useful model to further study the role of CDT and other aspects of C. jejuni pathogenesis... - Comparison of diagnostic techniques for Helicobacter cetorum infection in wild Atlantic bottlenose dolphins (Tursiops truncatus)Claudia G Harper
Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
J Clin Microbiol 41:2842-8. 2003..cetorum, and results indicate there is at least a 50% prevalence of H. cetorum infection in these dolphins... - Synergistic inhibitory effects of gastrin and histamine receptor antagonists on Helicobacter-induced gastric cancerShigeo Takaishi
Division of Digestive and Liver Disease, Department of Medicine, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA
Gastroenterology 128:1965-83. 2005..felis/INS-GAS mice, while the proton pump inhibitor showed no such effects. These results support an important role for the gastrin-histamine axis in Helicobacter-induced gastric carcinogenesis... - Cross-reactivity between immune responses to Helicobacter bilis and Helicobacter pylori in a population in Thailand at high risk of developing cholangiocarcinomaPaola Pisani
Descriptive Epidemiology Group, International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 03, France
Clin Vaccine Immunol 15:1363-8. 2008..We found high cross-reactivity between the immune responses to H. pylori and H. bilis antigens. More-specific H. bilis antigens need to be isolated to develop serologic tests suitable for epidemiological studies...