Research Topics
Species | Gordana Vunjak-NovakovicSummaryAffiliation: Massachusetts Institute of Technology Country: USA Publications
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Detail Information
Publications
Dynamic cell seeding of polymer scaffolds for cartilage tissue engineeringG Vunjak-Novakovic
Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Biotechnol Prog 14:193-202. 1998..The kinetics and possible mechanisms of cell seeding were related to the formation of cell aggregates by a simple mathematical model that can be used to optimize seeding conditions for cartilage tissue engineering...- Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue-engineered cartilageG Vunjak-Novakovic
Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge 02139, USA
J Orthop Res 17:130-8. 1999..These findings suggest that the hydrodynamic conditions in tissue-culture bioreactors can modulate the composition, morphology, mechanical properties, and electromechanical function of engineered cartilage... - Bioreactor cultivation of osteochondral graftsG Vunjak-Novakovic
Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Orthod Craniofac Res 8:209-18. 2005..Taken together, these studies provide a basis for the ongoing work on engineering osreochondral grafts for a variety of potential applications, including those in the craniofacial complex... - Tissue engineering of ligamentsG Vunjak-Novakovic
Massachusetts Institute of Technology, Harvard MIT Division of Health Sciences and Technology, Cambridge, Massachusetts 02139, USA
Annu Rev Biomed Eng 6:131-56. 2004..One representative tissue engineering system involving the integrated use of adult human stem cells, custom-designed scaffolds, and advanced bioreactors with dynamic loading is described... - Microgravity studies of cells and tissuesGordana Vunjak-Novakovic
Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Ann N Y Acad Sci 974:504-17. 2002.... - Bioreactor studies of native and tissue engineered cartilageG Vunjak-Novakovic
Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Biorheology 39:259-68. 2002.... 
Effects of chondrogenic and osteogenic regulatory factors on composite constructs grown using human mesenchymal stem cells, silk scaffolds and bioreactorsAlexander Augst
Harvard MIT Division of Health Sciences and Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
J R Soc Interface 5:929-39. 2008....- Engineering cartilage-like tissue using human mesenchymal stem cells and silk protein scaffoldsLorenz Meinel
Division of Health Sciences and Technology, Massachusetts Institute of Technology, E25-330, 45 Carleton Street, Cambridge, Massachusetts 02139, USA
Biotechnol Bioeng 88:379-91. 2004..Taken together, these results suggest that silk scaffolds are particularly suitable for tissue engineering of cartilage starting from hMSC, presumably due to their high porosity, slow biodegradation, and structural integrity... - Mechanical properties and remodeling of hybrid cardiac constructs made from heart cells, fibrin, and biodegradable, elastomeric knitted fabricJan Boublik
Division of Health Sciences and Technology, Center for Space Research, and Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, 02139, USA
Tissue Eng 11:1122-32. 2005..Together, the data showed that hybrid cardiac constructs initially exhibited supraphysiologic UTS, epsilon(f), and E, and remodeled in response to serum and stretch in vitro and in an ectopic in vivo model... - Medium perfusion enables engineering of compact and contractile cardiac tissueMilica Radisic
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139, USA
Am J Physiol Heart Circ Physiol 286:H507-16. 2004.... - Bone and cartilage tissue constructs grown using human bone marrow stromal cells, silk scaffolds and rotating bioreactorsDarja Marolt
Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, USA
Biomaterials 27:6138-49. 2006..Engineered bone constructs were large (8mm diameter x 2mm thick disks) and resembled trabecular bone with respect to structure and mineralized tissue volume fraction (12%)... - Engineering bone-like tissue in vitro using human bone marrow stem cells and silk scaffoldsLorenz Meinel
Division of Health Sciences and Technology, Massachusetts Institute of Technology, E25-330, 45 Carleton Street, Cambridge, Massachusetts 02139, USA
J Biomed Mater Res A 71:25-34. 2004.... - Practical aspects of cardiac tissue engineering with electrical stimulationChristopher Cannizzaro
Harvard MIT Division for Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, USA
Methods Mol Med 140:291-307. 2007.... - Microfluidic patterning for fabrication of contractile cardiac organoidsAli Khademhosseini
Harvard MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Biomed Microdevices 9:149-57. 2007..After 3 days in culture, the linearly aligned myocytes detached from the surface and formed contractile cardiac organoids. The procedure can be utilized to simply, rapidly and inexpensively create in vitro cardiac tissue models... - Bioactive hydrogel scaffolds for controllable vascular differentiation of human embryonic stem cellsLino S Ferreira
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Biomaterials 28:2706-17. 2007..Functionalized dextran-based hydrogels could thus enable derivation of vascular cells in large quantities, particularly endothelial cells, for potential application in tissue engineering and regenerative medicine... - Bone tissue engineering using human mesenchymal stem cells: effects of scaffold material and medium flowLorenz Meinel
Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Ann Biomed Eng 32:112-22. 2004..These results suggest that osteogenesis in cultured MSC can be modulated by scaffold properties and flow environment... - Pre-treatment of synthetic elastomeric scaffolds by cardiac fibroblasts improves engineered heart tissueMilica Radisic
Harvard MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
J Biomed Mater Res A 86:713-24. 2008..When implanted over the infarcted myocardium in a nude rat model, cell-free poly(glycerol sebacate) remained at the ventricular wall after 2 weeks of in vivo, and was vascularized... - Perfusion improves tissue architecture of engineered cardiac muscleRebecca L Carrier
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge 02139, USA
Tissue Eng 8:175-88. 2002..Medium perfusion could thus be utilized to better mimic the transport conditions within native cardiac muscle and enable in vitro engineering of cardiac constructs with clinically useful thicknesses... - A novel composite scaffold for cardiac tissue engineeringHyoungshin Park
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
In Vitro Cell Dev Biol Anim 41:188-96. 2005..Construct cellularity, presence of cardiac markers, and contractile properties were markedly improved in composite scaffolds as compared with both controls... - Bioreactors mediate the effectiveness of tissue engineering scaffoldsMing Pei
Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
FASEB J 16:1691-4. 2002..The data imply that interactions between bioreactors and 3D tissue engineering scaffolds can be utilized to improve the structure, function, and molecular properties of in vitro-generated cartilage... - A photolithographic method to create cellular micropatternsJeffrey M Karp
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, 02139, USA
Biomaterials 27:4755-64. 2006..Osteoblasts (SAOS-2) localized in the exposed glass regions (squares, triangles, or circles; 0.063-0.5mm(2)). They proliferated to confluence in 5 days, expressed alkaline phosphatase and produced a mineralized matrix... - Vascular progenitor cells isolated from human embryonic stem cells give rise to endothelial and smooth muscle like cells and form vascular networks in vivoLino S Ferreira
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Circ Res 101:286-94. 2007.... - The inflammatory responses to silk films in vitro and in vivoLorenz Meinel
Division of Health Sciences and Technology, Massachusetts Institute of Technology, E25-330, 45 Carleton Street, Cambridge, MA 02139, USA
Biomaterials 26:147-55. 2005..These data suggest that (a) purified degradable silk is biocompatible and (b) the in vitro cell culture model (hMSC seeded and cultured on biomaterial films) gave inflammatory responses that were comparable to those observed in vivo... - High-density seeding of myocyte cells for cardiac tissue engineeringMilica Radisic
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Biotechnol Bioeng 82:403-14. 2003..Direct perfusion can thus enable seeding of hypoxia-sensitive cells at physiologically high and spatially uniform initial densities and maintain cell viability and function... - Development and remodeling of engineered cartilage-explant composites in vitro and in vivoEnrico Tognana
Division of Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Osteoarthritis Cartilage 13:896-905. 2005..CONCLUSIONS: Development and remodeling of composites based on engineered cartilage were mediated in vitro by cell chondrogenic potential and scaffold degradation rate, and in vivo by type of adjacent tissue and time... - Functional assembly of engineered myocardium by electrical stimulation of cardiac myocytes cultured on scaffoldsMilica Radisic
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E25-342, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 101:18129-34. 2004..Development of conductive and contractile properties of cardiac constructs was concurrent, with strong dependence on the initiation and duration of electrical stimulation... - The fundamentals of tissue engineering: scaffolds and bioreactorsGordana Vunjak-Novakovic
Massachusetts Institute of Technology, Harvard-MIT Division of Health Sciences and Technology, Cambridge MA 02139, USA
Novartis Found Symp 249:34-46; discussion 46-51, 170-4, 239-41. 2003..Here we explore the paradigm of tissue-engineered cartilage repair that is based on the generation of immature but functional constructs in vitro, and the remodelling and maturation of these constructs in vivo... - Oxygen gradients correlate with cell density and cell viability in engineered cardiac tissueMilica Radisic
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Biotechnol Bioeng 93:332-43. 2006..Medium flow significantly increased oxygen concentration within the construct, correlating with the improved tissue properties observed for constructs cultured in convectively mixed bioreactors... - Cell seeding of polymer scaffoldsGordana Vunjak-Novakovic
Harvard-MIT Division of Health Science and Technology, MIT, Cambridge, MA, USA
Methods Mol Biol 238:131-46. 2004 - Advanced tools for tissue engineering: scaffolds, bioreactors, and signalingLisa E Freed
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Tissue Eng 12:3285-305. 2006.... - Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cellsSharon Gerecht
Harvard Massachusetts Institute of Technology, Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 104:11298-303. 2007..We therefore propose that HA hydrogels, with their developmentally relevant composition and tunable physical properties, provide a unique microenvironment for the self-renewal and differentiation of hESCs... - Effects of electrical stimulation in C2C12 muscle constructsHyoungshin Park
Health Science and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA
J Tissue Eng Regen Med 2:279-87. 2008..Our data implicate that a specific electrical frequency may modulate type I collagen accumulation and a specific voltage may affect the differentiation of muscle sarcomeres in excitable cells... - Cultivation in rotating bioreactors promotes maintenance of cardiac myocyte electrophysiology and molecular propertiesNenad Bursac
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Tissue Eng 9:1243-53. 2003.... - Mathematical model of oxygen distribution in engineered cardiac tissue with parallel channel array perfused with culture medium containing oxygen carriersMilica Radisic
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Am J Physiol Heart Circ Physiol 288:H1278-89. 2005..5 cm). In future work, the model can be utilized as a tool for optimization of scaffold geometry and flow conditions... - Spaceflight bioreactor studies of cells and tissuesLisa E Freed
Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA
Adv Space Biol Med 8:177-95. 2002..The increasing scientific and medical relevance of this work is evidenced by the growing number of publications in which advanced bioreactors are used for in vitro studies in physiologically relevant cell and tissue models... - Nanofabrication and microfabrication of functional materials for tissue engineeringHyoungshin Park
Division of Health Sciences and Technology, Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Tissue Eng 13:1867-77. 2007..It is our opinion that these novel materials and technologies will bring engineered tissues one step closer to practical application in the clinic. This review discusses their application to cardiac, liver, and nerve tissue engineering... - Effects of oxygen on engineered cardiac muscleRebecca L Carrier
Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Biotechnol Bioeng 78:617-25. 2002..Experiments of this kind can form a basis for controlled studies of the effects of oxygen on the in vitro development of engineered tissues... - Differential effects of growth factors on tissue-engineered cartilageTorsten Blunk
Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Tissue Eng 8:73-84. 2002..Different regulatory factors thus elicit significantly different chondrogenic responses and can be used to selectively control the growth rate and improve the composition of engineered cartilage... - Engineering tissue with BioMEMSJeffrey T Borenstein
Biomedical Engineering Center, Draper Laboratory, Cambridge, Massachusetts, USA
IEEE Pulse 2:28-34. 2011.... - Engineering custom-designed osteochondral tissue graftsWarren L Grayson
Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
Trends Biotechnol 26:181-9. 2008.... - It takes a village to grow a tissueDavid L Kaplan
Nat Biotechnol 23:1237-9. 2005 - Cell differentiation by mechanical stressGregory H Altman
Department of Chemical and Biological Engineering, Bioengineering Center, Tufts University, Medford, Massachusetts 02155, USA
FASEB J 16:270-2. 2002..At the same time, no up-regulation of bone or cartilage-specific cell markers was observed... - Non-invasive time-lapsed monitoring and quantification of engineered bone-like tissueHenri Hagenmüller
Institute for Biomechanics, ETH Zurich, 8093, Zurich, Switzerland
Ann Biomed Eng 35:1657-67. 2007..Our data demonstrate the feasibility of qualitatively and quantitatively detailing the spatial and temporal mineralization of bone-like tissue formation in tissue engineering... - Advanced bioreactor with controlled application of multi-dimensional strain for tissue engineeringGregory H Altman
Tufts University, Department of Chemical and Biological Engineering, Bioengineering Center, 4 Colby Street, Medford, MA 02155, USA
J Biomech Eng 124:742-9. 2002..5%) over 14 days in culture. The system supported cell spreading and growth on the silk fiber matrices based on SEM characterization, as well as the differentiation of the cells into ligament-like cells and tissue (Altman et al., 2001)... - Engineering cartilage and bone using human mesenchymal stem cellsPen-Hsiu Grace Chao
Department of Biomedical Engineering, Columbia University, 363G Engineering Terrace, New York, NY 10027, USA
J Orthop Sci 12:398-404. 2007 - Silk fibroin microtubes for blood vessel engineeringMichael Lovett
Department of Biomedical Engineering, Tufts University, 4 Colby St, Medford, MA 02155, USA
Biomaterials 28:5271-9. 2007..When combined with the biocompatible and suturability features of silk fibroin, these results suggest that silk microtubes, either implanted directly or preseeded with cells, are an attractive biomaterial for microvascular grafts... - Cardiac tissue engineering using perfusion bioreactor systemsMilica Radisic
Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Ontario, Canada
Nat Protoc 3:719-38. 2008..This model is well suited for a wide range of cardiac tissue engineering applications, including the use of human stem cells, and high-fidelity models for biological research... - Effect of scaffold design on bone morphology in vitroLorenz Uebersax
Institute for Pharmaceutical Sciences, ETH Zurich, Zurich, Switzerland
Tissue Eng 12:3417-29. 2006..The ability to direct bone morphology via scaffold design suggests new options in the use of biodegradable scaffolds to control in vitro engineered bone tissue outcomes... - Engineering complex tissuesAntonios G Mikos
Department of Bioengineering, Rice University, Houston, Texas, USA
Tissue Eng 12:3307-39. 2006..Anthony Atala offered a clinician's perspective for functional tissue regeneration, and discussed new biomaterials that can be used to develop new regenerative technologies... - Micro-bioreactor array for controlling cellular microenvironmentsElisa Figallo
Department of Chemical Engineering, University of Padova, Italy
Lab Chip 7:710-9. 2007..To illustrate the utility of the MBA for controlled studies of hESCs, we established correlations between the expression of smooth muscle actin and cell density for three different flow configurations... - Osteogenesis by human mesenchymal stem cells cultured on silk biomaterials: comparison of adenovirus mediated gene transfer and protein delivery of BMP-2Lorenz Meinel
Department of Chemistry and Applied Biosciences, ETH Zuerich, Wolfgang-Pauli Str. 10, 8093 Zuerich, Switzerland
Biomaterials 27:4993-5002. 2006..These findings suggest additional options to control differentiation where exogenous additions of growth factors or morphogens can be replaced with transfected MSCs... - Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffoldsSandra Hofmann
Institute of Pharmaceutical Sciences, HCI J 392, Wolfgang Pauli Strasse 10, ETH Zurich, 8093 Zurich, Switzerland
Biomaterials 28:1152-62. 2007..Micro-computed tomography (microCT) detailed the pore structures of the newly formed tissue and suggested that the structure of tissue-engineered bone was controlled by the underlying scaffold geometry... - Influence of macroporous protein scaffolds on bone tissue engineering from bone marrow stem cellsHyeon Joo Kim
Department of Biomedical Engineering, Bioengineering Center, Tufts University, Medford, MA 02155, USA
Biomaterials 26:4442-52. 2005..These data illustrate the importance of materials processing on biological outcomes, as the same protein, silk fibroin, was used in both preparations... - Porous silk fibroin 3-D scaffolds for delivery of bone morphogenetic protein-2 in vitro and in vivoVassilis Karageorgiou
Department of Biomedical Engineering Chemical and Biological Engineering, Tufts University, 4 Colby Street, Medford, Massachusetts 02155, USA
J Biomed Mater Res A 78:324-34. 2006.... - Stem cell-based tissue engineering with silk biomaterialsYongzhong Wang
Department of Chemical and Biological Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA
Biomaterials 27:6064-82. 2006.... - Silk implants for the healing of critical size bone defectsLorenz Meinel
Department of Biomedical Engineering, Tufts University, 4 Colby Street, Medford, MA 02155, USA
Bone 37:688-98. 2005..These results demonstrate the feasibility of silk-based implants with engineered bone for the (re-)generation of bone tissues and expand the class of protein-based bone-implant materials with a mechanically stable and durable option... - Cartilage-like tissue engineering using silk scaffolds and mesenchymal stem cellsSandra Hofmann
Institute of Pharmaceutical Sciences, ETH Zurich, Zurich, Switzerland
Tissue Eng 12:2729-38. 2006.... - Space Station Biological Research Project (SSBRP) Cell Culture Unit (CCU) and incubator for International Space Station (ISS) cell culture experimentsDonald Vandendriesche
NASA Ames Research Center, Moffett Field, CA 94035, USA
J Gravit Physiol 11:93-103. 2004.... - Gene transfer of a human insulin-like growth factor I cDNA enhances tissue engineering of cartilageHenning Madry
Department of Orthopedic Surgery, Harvard Medical School, Massachusetts General Hospital, Boston 02114, USA
Hum Gene Ther 13:1621-30. 2002.... - Engineering of functional cartilage tissue using stem cells from synovial lining: a preliminary studyMing Pei
Tissue Engineering Laboratory, Department of Orthopaedics, West Virginia University, 3943 Health Sciences Center South, PO Box 9196, Morgantown, WV 26506 9196, USA
Clin Orthop Relat Res 466:1880-9. 2008..We thus propose functional cartilage constructs could be engineered in vitro through the use of negatively isolated SDSCs... 
Tissue engineering by self-assembly of cells printed into topologically defined structuresKaroly Jakab
Department of Physics, University of Missouri, Columbia, Missouri 65211, USA
Tissue Eng Part A 14:413-21. 2008..The postprinting self-assembly of bio-ink particles resulted in synchronously beating solid tissue blocks, showing signs of early vascularization, with the endothelial cells organized into vessel-like conduits...- Characterization of electrical stimulation electrodes for cardiac tissue engineeringNina Tandon
Department of Biomedical Engineering, Columbia University, 1210 Amsterdam Avenue, 353 Engineering Terrace, New York, NY 10027, USA
Conf Proc IEEE Eng Med Biol Soc 1:845-8. 2006.... - Growth factor induced fibroblast differentiation from human bone marrow stromal cells in vitroJodie E Moreau
Department of Biomedical Engineering, Bioengineering Center, Tufts University, 4 Colby Street, Medford, MA 02155, USA
J Orthop Res 23:164-74. 2005.... - Tissue-engineered composites for the repair of large osteochondral defectsDirk Schaefer
University of Basel, Basel, Switzerland
Arthritis Rheum 46:2524-34. 2002..37 MPa) was significantly lower. CONCLUSION: Composites of tissue-engineered cartilage and a subchondral support promote the orderly remodeling of large osteochondral defects in adult rabbits... - The effect of actin disrupting agents on contact guidance of human embryonic stem cellsSharon Gerecht
Harvard M I T Division of Health Sciences and Technology, USA
Biomaterials 28:4068-77. 2007..These findings further demonstrate the importance of interplay between cytoskeleton and substrate interactions as a key modulator of morphological and proliferative cellular response in hESCs on nanotopography... - Osteogenic differentiation of human bone marrow stromal cells on partially demineralized bone scaffolds in vitroJoshua R Mauney
Department of Chemical and Biological Engineering, Biotechnology Center, Tufts University, Medford, Massachusetts, USA
Tissue Eng 10:81-92. 2004..This osseous biomaterial may offer new potential benefits as a tool for clinical bone replacement... - Synthetic oxygen carriers in cardiac tissue engineeringRohin K Iyer
University of Toronto, IBBME, Department of Chemical Engineering and Applied Chemistry, Toronto, Ontario, Canada
Artif Cells Blood Substit Immobil Biotechnol 35:135-48. 2007..The presence of PFC enhanced the transport of oxygen, increased oxygen concentrations, and yielded constructs that displayed stronger cardiac-like phenotype... - Tissue engineering: the next generationGordana Vunjak-Novakovic
Department of Biomedical Engineering, Columbia University, New York, NY 10027, USA
Tissue Eng 12:3261-3. 2006 - Translation from research to applicationsErnst Hunziker
ITI Research Institute for Dental and Skeletal Biology, University of Bern, Bern, Switzerland
Tissue Eng 12:3341-64. 2006....