Research Topics
| Manish SagarSummaryAffiliation: Massachusetts General Hospital Country: USA Publications
Research Grants
| Collaborators
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Detail Information
Publications
Transmembrane domain membrane proximal external region but not surface unit-directed broadly neutralizing HIV-1 antibodies can restrict dendritic cell-mediated HIV-1 trans-infectionManish Sagar
Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Cambridge, MA 02139, USA
J Infect Dis 205:1248-57. 2012....
Diversity in HIV-1 envelope V1-V3 sequences early in infection reflects sequence diversity throughout the HIV-1 genome but does not predict the extent of sequence diversity during chronic infectionManish Sagar
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
AIDS Res Hum Retroviruses 22:430-7. 2006..Analysis of HIV-1 sequences from about 3 years after infection revealed that the level of diversity and diversification was similar between the women in the two groups...- Selection of HIV variants with signature genotypic characteristics during heterosexual transmissionManish Sagar
Brigham and Women s Hospital, Boston, Massachusetts, USA
J Infect Dis 199:580-9. 2009..Newly infected subjects acquire a limited number of human immunodeficiency virus type 1 (HIV-1) variants with specific genotypic and phenotypic features from the array of viruses present in a chronically infected transmitting partner... - HIV-1 transmission biology: selection and characteristics of infecting virusesManish Sagar
Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts 02139, USA
J Infect Dis 202:S289-96. 2010..Greater understanding of these issues may open promising new ways to effectively block HIV-1 transmission... - Effect of contraceptive methods on natural history of HIV: studies from the Mombasa cohortJared M Baeten
University of Washington, Seattle, WA, USA
J Acquir Immune Defic Syndr 38:S18-21. 2005 - Selection for human immunodeficiency virus type 1 envelope glycosylation variants with shorter V1-V2 loop sequences occurs during transmission of certain genetic subtypes and may impact viral RNA levelsBhavna Chohan
Division of Human Biology, Fred Hutchinson Cancer Center, 1100 Fairview Ave. N, C3-168 Seattle, WA 98109-1024, USA
J Virol 79:6528-31. 2005..Thus, our study also suggests that the extent of glycosylation in the infecting virus could impact disease progression... - Evolution of CCR5 antagonist resistance in an HIV-1 subtype C clinical isolateTimothy J Henrich
Division of Infectious Diseases, Brigham and Women s Hospital, Boston, MA, USA
J Acquir Immune Defic Syndr 55:420-7. 2010..Here, we test the contribution of gp120 mutations to CCR5 antagonist resistance and investigate why certain minority V3 variants emerged as the dominant species under drug pressure... - Identification of modifiable factors that affect the genetic diversity of the transmitted HIV-1 populationManish Sagar
Division of Human Biology, Fred Hutchinson Cancer Research Center, University of Washington, 1100 Fairview Avenue, Seattle, WA 98109, USA
AIDS 18:615-9. 2004..CONCLUSION: The presence of genital tract infections and hormonal contraceptive use at the time of transmission were associated with the acquisition of multiple HIV-1 variants... 
Env sequence determinants in CXCR4-using human immunodeficiency virus type-1 subtype CNina H Lin
Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA
Virology 433:296-307. 2012..Chimeric env functional studies suggest that the V3 loop is necessary but often not sufficient to impart CXCR4 utilization. Our studies provide insights into the unique genotypic characteristics of X4 variants in HIV-1C...- Human immunodeficiency virus type 1 V1-V2 envelope loop sequences expand and add glycosylation sites over the course of infection, and these modifications affect antibody neutralization sensitivityManish Sagar
Department of Medicine, Brigham and Women's Hospital, Cambridge, Massachusetts 02139, USA
J Virol 80:9586-98. 2006..These studies suggest that changes within the V1-V2 envelope domains over the course of an infection influence sensitivity to autologous neutralizing antibodies and may also impact host receptor/coreceptor interactions... - Human immunodeficiency virus type 1 V1-to-V5 envelope variants from the chronic phase of infection use CCR5 and fuse more efficiently than those from early after infectionBehzad Etemad
Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Cambridge, Massachusetts 02139, USA
J Virol 83:9694-708. 2009..Thus, envelope modifications over time within a host potentially enhance replication capacity... - No evidence for rapid subtype C spread within an epidemic in which multiple subtypes and intersubtype recombinants circulateStephanie Rainwater
Division of Human Biology, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
AIDS Res Hum Retroviruses 21:1060-5. 2005..This latter finding suggests that reinfection may have occurred in high-risk groups early in the epidemic, leading to intersubtype recombinant viruses that underwent secondary spread... - In vivo emergence of vicriviroc resistance in a human immunodeficiency virus type 1 subtype C-infected subjectAthe M N Tsibris
Massachusetts General Hospital, Boston, Massachusetts, USA
J Virol 82:8210-4. 2008..Pretreatment V3 loop sequences reemerged following VCV discontinuation, implying that VCV resistance has associated fitness costs... - In vivo fitness cost of the M184V mutation in multidrug-resistant human immunodeficiency virus type 1 in the absence of lamivudineRoger Paredes
Brigham and Women s Hospital, Harvard Medical School, Boston, Massachusetts, USA
J Virol 83:2038-43. 2009..After a lag phase of variable duration, wild-type variants emerged due to continued evolution of pol and back mutation rather than through emergence of an archived wild-type variant... - Human immunodeficiency virus type 1 (HIV-1) diversity at time of infection is not restricted to certain risk groups or specific HIV-1 subtypesManish Sagar
Division of Human Biology, Fred Hutchinson Cancer Research Center, and Department of Medicine, University of Washington, Seattle, USA
J Virol 78:7279-83. 2004..S. women, one U.S. man, and one Kenyan man near the time of seroconversion. These findings suggest that early HIV-1 genetic diversity is not exclusive to women from Africa or to infection with any particular HIV-1 subtype... - Infection with multiple human immunodeficiency virus type 1 variants is associated with faster disease progressionManish Sagar
Divisions of Human Biology, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, Washington 98109, USA
J Virol 77:12921-6. 2003..01) 4 to 24 months after infection compared to women who were infected with a single viral genotype. These studies suggest that early HIV-1 genetic diversity is linked to faster disease progression... - Sensitivity changes over the course of infection increases the likelihood of resistance against fusion but not CCR5 receptor blockersNikolaos Chatziandreou
Brigham and Women s Hospital, Boston, MA 02139, USA
AIDS Res Hum Retroviruses 28:1584-93. 2012..Our in vitro passage studies suggest that these evolving phenotypes increase the likelihood of resistance against fusion but not CCR5 receptor blockers...
Research Grants
- The role of viral diversity in HIV-1 drug resistanceManish Sagar; Fiscal Year: 2006..With these studies, we will gain insight into the likelihood of success with ART in Africa and the possibility of the evolution of a 'new' epidemic with drug resistant HIV -1 viruses. ..
- Genotypic and Phenotypic Characterization of Heterosexually Transmitted HIV-1Manish Sagar; Fiscal Year: 2010..We will explore the biological relevance of observed differences in these phenotypic properties to develop potential biological models for selection during HIV-1 transmission. ..