Elizabeth M Nolan

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. Lee H, Savelieff M, Kang J, Brophy M, Nakashige T, Lee S, et al. Calprotectin influences the aggregation of metal-free and metal-bound amyloid-β by direct interaction. Metallomics. 2018;10:1116-1127 pubmed publisher
    ..In contrast, S100B has a slight effect on the aggregation of Aβ. Overall, this work supports a potential association of CP with Aβ in the absence and presence of metal ions in AD. ..
  2. Chairatana P, Zheng T, Nolan E. Targeting virulence: salmochelin modification tunes the antibacterial activity spectrum of ?-lactams for pathogen-selective killing of Escherichia coli. Chem Sci. 2015;6:4458-4471 pubmed publisher
  3. Zygiel E, Nolan E. Transition Metal Sequestration by the Host-Defense Protein Calprotectin. Annu Rev Biochem. 2018;87:621-643 pubmed publisher
  4. Sassone Corsi M, Chairatana P, Zheng T, Perez Lopez A, Edwards R, George M, et al. Siderophore-based immunization strategy to inhibit growth of enteric pathogens. Proc Natl Acad Sci U S A. 2016;113:13462-13467 pubmed
  5. Baker T, Nakashige T, Nolan E, Neidig M. Magnetic circular dichroism studies of iron(ii) binding to human calprotectin. Chem Sci. 2017;8:1369-1377 pubmed publisher
    ..These spectroscopic studies provide further evaluation of a unique non-heme Fe(ii)-His6 site for metalloproteins and support the notion that Ca(ii) ions influence the Fe(ii)-binding properties of CP. ..
  6. Zygiel E, Nelson C, Brewer L, Oglesby Sherrouse A, Nolan E. The human innate immune protein calprotectin induces iron starvation responses in Pseudomonas aeruginosa. J Biol Chem. 2019;294:3549-3562 pubmed publisher
    ..This work implicates CP-mediated Fe(II) sequestration as a component of nutritional immunity in both aerobic and anaerobic milieus during P. aeruginosa infection. ..
  7. Cunden L, Nolan E. Preparation of the Oxidized and Reduced Forms of Psoriasin (S100A7). Methods Mol Biol. 2019;1929:379-395 pubmed publisher
    ..A RP-HPLC method that allows for baseline separation of S100A7ox and S100A7red is provided, as well as a biochemical Zn(II)-binding assay that can be employed to evaluate the functional integrity of S100A7. ..
  8. Hadley R, Nolan E. Preparation and Iron Redox Speciation Study of the Fe(II)-Binding Antimicrobial Protein Calprotectin. Methods Mol Biol. 2019;1929:397-415 pubmed publisher
    ..An Fe redox speciation assay employing ferrozine is described that demonstrates the ability of both the human and murine orthologues of CP to shift the redox speciation of Fe from the ferric to the ferrous oxidation state over time. ..
  9. Neumann W, Nolan E. Evaluation of a reducible disulfide linker for siderophore-mediated delivery of antibiotics. J Biol Inorg Chem. 2018;23:1025-1036 pubmed publisher

More Information

Publications14

  1. Neumann W, Gulati A, Nolan E. Metal homeostasis in infectious disease: recent advances in bacterial metallophores and the human metal-withholding response. Curr Opin Chem Biol. 2017;37:10-18 pubmed publisher
    ..The studies also underscore that the chemical composition of the microenvironment at an infection site can influence bacterial pathogenesis and the innate immune system. ..
  2. Johnstone T, Nolan E. Beyond iron: non-classical biological functions of bacterial siderophores. Dalton Trans. 2015;44:6320-39 pubmed publisher
  3. Zheng T, Nolan E. Evaluation of (acyloxy)alkyl ester linkers for antibiotic release from siderophore-antibiotic conjugates. Bioorg Med Chem Lett. 2015;25:4987-4991 pubmed publisher
    ..The hydrolytic stabilities of ciprofloxacin-modified (acyloxy)alkyl esters, evaluated at pH 7.5 and 30°C, vary by >370-fold depending on the substituents in the vicinity of the ester linkage. ..
  4. Neumann W, Hadley R, Nolan E. Transition metals at the host-pathogen interface: how Neisseria exploit human metalloproteins for acquiring iron and zinc. Essays Biochem. 2017;61:211-223 pubmed publisher
  5. Nakashige T, Nolan E. Human calprotectin affects the redox speciation of iron. Metallomics. 2017;9:1086-1095 pubmed publisher
    ..These findings indicate that the presence of microbial metabolites that contribute to metal homeostasis at the host/pathogen interface can affect the metal-sequestering function of CP. ..