Michael T McManus

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. ncbi request reprint Gene silencing in mammals by small interfering RNAs
    Michael T McManus
    Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street E17 526, Cambridge, Massachusetts 02139, USA
    Nat Rev Genet 3:737-47. 2002
  2. ncbi request reprint MicroRNAs and cancer
    Michael T McManus
    Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street, Cambridge, MA 02139, USA
    Semin Cancer Biol 13:253-8. 2003
  3. ncbi request reprint A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference
    Douglas A Rubinson
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Genet 33:401-6. 2003
  4. ncbi request reprint Small interfering RNA-mediated gene silencing in T lymphocytes
    Michael T McManus
    Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street E17 526, Cambridge, MA 02139, USA
    J Immunol 169:5754-60. 2002
  5. pmc RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription
    Qing Ge
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 100:2718-23. 2003
  6. pmc Dicer1 and miR-219 Are required for normal oligodendrocyte differentiation and myelination
    Jason C Dugas
    Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305 5125, USA
    Neuron 65:597-611. 2010
  7. ncbi request reprint TAZ, a transcriptional modulator of mesenchymal stem cell differentiation
    Jeong Ho Hong
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Science 309:1074-8. 2005
  8. ncbi request reprint Lentiviral strategies for RNAi knockdown of neuronal genes
    Jill R Crittenden
    Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Curr Protoc Neurosci . 2007
  9. pmc Gene silencing using micro-RNA designed hairpins
    Michael T McManus
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA
    RNA 8:842-50. 2002
  10. pmc The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb
    Brian D Harfe
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 102:10898-903. 2005

Collaborators

  • Jianzhu Chen
  • Christopher P Dillon
  • Qing Ge
  • Elisabetta Mueller
  • Eun Sook Hwang
  • Jeong Ho Hong
  • Adam Amsterdam
  • Jason C Dugas
  • Brian B Haines
  • Ben Emery
  • Michael B Yaffe
  • BRIAN DAVID HARFE
  • David Bartel
  • Amy E Pasquinelli
  • GARY B RUVKUN
  • Trinna L Cuellar
  • Jennifer H Mansfield
  • Ivan Osokine
  • Gregory Ku
  • Xuyu Zhou
  • Scott J Harvey
  • Tigwa H Davis
  • Jason R O'Rourke
  • Francis C Lynn
  • Jill R Crittenden
  • Yong Zhao
  • Eran Hornstein
  • John P Morris
  • Clifford J Tabin
  • Douglas A Rubinson
  • Brian T Fife
  • Mark S Anderson
  • Allison J Barker
  • Bernhard Schermer
  • Jeffrey A Bluestone
  • Jeffrey H Miner
  • Gabriel B Loeb
  • Selina M Koch
  • Erik M Ullian
  • Thomas Benzing
  • Ruby Hsu
  • Shirley Zhu
  • Seth Goldberg
  • Lukas T Jeker
  • Jeanette Cunningham
  • George Jarad
  • Ankang Li
  • Joshua F Ransom
  • Peter Skewes-Cox
  • Deepak Srivastava
  • Amy Heidersbach
  • Morgan von Drehle
  • Robert J Schwartz
  • Sara A Georges
  • Alecia N Muth
  • David J Goldhamer
  • Stephen J Tapscott
  • Takatoshi Tsuchihashi
  • Yasuhiro Kosaka
  • Maurice S Swanson
  • Michael S German
  • Vasanth Vedantham
  • Howard R Seay
  • Jimmy Kuang Hsien Hu
  • Soraya Yekta
  • Scott Baskerville
  • Robert Nissen
  • Phillip A Sharp
  • John Obenauer
  • Jalagani Srineel
  • Raphael Farzan-Kashani
  • Aadel Chaudhuri
  • Michael Zuker
  • Claudia Sievers
  • Luk Van Parijs
  • Frank B Gertler
  • Mingdi Zhang
  • Lili Yang
  • Dina L Rooney
  • Martin L Scott
  • Johnny Kopinja
  • Melanie M Ihrig
  • Adam V Kwiatkowski

Detail Information

Publications24

  1. ncbi request reprint Gene silencing in mammals by small interfering RNAs
    Michael T McManus
    Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street E17 526, Cambridge, Massachusetts 02139, USA
    Nat Rev Genet 3:737-47. 2002
    ..Although the use of siRNAs to silence genes in vertebrate cells was only reported a year ago, the emerging literature indicates that most vertebrate genes can be studied with this technology...
  2. ncbi request reprint MicroRNAs and cancer
    Michael T McManus
    Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street, Cambridge, MA 02139, USA
    Semin Cancer Biol 13:253-8. 2003
    ..Recent data suggests that a special class of ncRNAs called microRNAs might be involved in human disease. This review proposes a role for microRNAs in oncogenesis...
  3. ncbi request reprint A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference
    Douglas A Rubinson
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Genet 33:401-6. 2003
    ..They may also provide new approaches for gene therapy...
  4. ncbi request reprint Small interfering RNA-mediated gene silencing in T lymphocytes
    Michael T McManus
    Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street E17 526, Cambridge, MA 02139, USA
    J Immunol 169:5754-60. 2002
    ..Using primary differentiated T lymphocytes, we provide the first evidence of siRNA-mediated RNAi gene silencing in normal nontransformed somatic mammalian lymphocytes...
  5. pmc RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription
    Qing Ge
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 100:2718-23. 2003
    ..The findings reveal that newly synthesized NP and PA proteins are required for influenza virus transcription and replication and provide a basis for the development of siRNAs as prophylaxis and therapy for influenza infection in humans...
  6. pmc Dicer1 and miR-219 Are required for normal oligodendrocyte differentiation and myelination
    Jason C Dugas
    Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305 5125, USA
    Neuron 65:597-611. 2010
    ..Together, these findings show that miR-219 plays a critical role in coupling differentiation to proliferation arrest in the OL lineage, enabling the rapid transition from proliferating OPCs to myelinating OLs...
  7. ncbi request reprint TAZ, a transcriptional modulator of mesenchymal stem cell differentiation
    Jeong Ho Hong
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Science 309:1074-8. 2005
    ..These results indicate that TAZ functions as a molecular rheostat that modulates MSC differentiation...
  8. ncbi request reprint Lentiviral strategies for RNAi knockdown of neuronal genes
    Jill R Crittenden
    Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Curr Protoc Neurosci . 2007
    ..Additional protocols describe production and titering of the lentivirus, as well as safety testing. Finally, methods are provided for infecting neurons in culture and in vivo with RNAi lentivirus...
  9. pmc Gene silencing using micro-RNA designed hairpins
    Michael T McManus
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA
    RNA 8:842-50. 2002
    ..Furthermore, these RNAs are active when expressed by DNA vectors containing polymerase III promoters, opening the possibility for new approaches in stable RNAi-based loss of function studies...
  10. pmc The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limb
    Brian D Harfe
    Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA
    Proc Natl Acad Sci U S A 102:10898-903. 2005
    ..Thus, Dicer is required for the formation of normal mouse limbs. Strikingly, however, we did not detect defects in basic patterning or in tissue-specific differentiation of Dicer-deficient limb buds...
  11. pmc Podocyte-specific deletion of dicer alters cytoskeletal dynamics and causes glomerular disease
    Scott J Harvey
    Renal Division, Washington University School of Medicine, St Louis, MO 63110, USA
    J Am Soc Nephrol 19:2150-8. 2008
    ..The unique architecture of podocytes may make them especially susceptible to cytoskeletal alterations initiated by aberrant miRNA dynamics...
  12. pmc Selective miRNA disruption in T reg cells leads to uncontrolled autoimmunity
    Xuyu Zhou
    Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA
    J Exp Med 205:1983-91. 2008
    ..These results support a central role for miRNAs in maintaining the stability of differentiated T reg cell function in vivo and homeostasis of the adaptive immune system...
  13. pmc Conditional loss of Dicer disrupts cellular and tissue morphogenesis in the cortex and hippocampus
    Tigwa H Davis
    Department of Ophthalmology, University of California, San Francisco, San Francisco, California 94143 0730, USA
    J Neurosci 28:4322-30. 2008
    ....
  14. ncbi request reprint MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expression
    Jennifer H Mansfield
    Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Genet 36:1079-83. 2004
    ..Furthermore, miR-196a negatively regulates Hoxb8, indicating that its restricted expression pattern probably reflects a role in the patterning function of the Hox complex...
  15. pmc Michael T. McManus: Interrupting biology [interview by Hema Bashyam]
    Michael T McManus
    J Exp Med 205:506-7. 2008
  16. pmc Unintentional miRNA ablation is a risk factor in gene knockout studies: a short report
    Ivan Osokine
    Department of Microbiology and Immunology, University of California San Francisco Diabetes Center, University of California San Francisco, San Francisco, California, United States of America
    PLoS Genet 4:e34. 2008
    ..These data also raise the possibility that many knockout studies may need to be reexamined to determine if loss of a microRNA contributes to the phenotypic consequences attributed to loss of a protein-encoding gene...
  17. doi request reprint Behind the scenes of a small RNA gene-silencing pathway
    Gregory Ku
    Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Francisco, San Francisco, CA 94122, USA
    Hum Gene Ther 19:17-26. 2008
    ..This review discusses advances in the biogenesis of miRNAs, their modes of action, and implications for RNA-based treatments...
  18. pmc Essential role for Dicer during skeletal muscle development
    Jason R O'Rourke
    Department of Molecular Genetics and Microbiology and the Genetics Institute, University of Florida, College of Medicine, Gainesville, FL 32610 0266, USA
    Dev Biol 311:359-68. 2007
    ..These observations demonstrate key roles for Dicer in skeletal muscle and implicate miRNAs as critical components required for embryonic myogenesis...
  19. ncbi request reprint MicroRNA expression is required for pancreatic islet cell genesis in the mouse
    Francis C Lynn
    Diabetes Center, Hormone Research Institute, University of California San Francisco, 513 Parnassus Ave, San Francisco, CA 94143 0534, USA
    Diabetes 56:2938-45. 2007
    ..We tested the hypothesis that microRNAs (miRNAs), which limit gene expression through posttranscriptional silencing, modulate the gene expression cascades involved in pancreas development...
  20. ncbi request reprint Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2
    Yong Zhao
    Gladstone Institute of Cardiovascular Disease, 1650 Owens Street, San Francisco, CA 94158, USA
    Cell 129:303-17. 2007
    ..These findings indicate that subtle alteration of miRNA dosage can have profound consequences in mammals and demonstrate the utility of mammalian loss-of-function models in revealing physiologic miRNA targets...
  21. ncbi request reprint Small RNAs and immunity
    Michael T McManus
    UCSF Diabetes Center, Department of Microbiology and Immunology, University of California, San Francisco, CA 94122, USA
    Immunity 21:747-56. 2004
    ..This review discusses the potential roles of small RNAs in immune biology and speculates on their potential participation in lymphogenesis and antiviral mechanisms...
  22. ncbi request reprint The microRNA miR-196 acts upstream of Hoxb8 and Shh in limb development
    Eran Hornstein
    Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
    Nature 438:671-4. 2005
    ....
  23. ncbi request reprint Slowing down the Ras lane: miRNAs as tumor suppressors?
    John P Morris
    UCSF Diabetes Center, Department of Microbiology and Immunology, University of California, San Francisco, CA 94122 0534, USA
    Sci STKE 2005:pe41. 2005
    ....
  24. ncbi request reprint MicroRNAs and endocrine biology
    Trinna L Cuellar
    UCSF Diabetes Center, Department of Microbiology and Immunology, University of California, San Francisco, California 94122 0534, USA
    J Endocrinol 187:327-32. 2005
    ..This review provides a general background regarding current knowledge about miRNA biogenesis and the potential contributions of these RNAs to endocrine function...