Genomes and Genes
Michael T McManus
Affiliation: Massachusetts Institute of Technology
- MicroRNAs and cancerMichael T McManus
Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street, Cambridge, MA 02139, USA
Semin Cancer Biol 13:253-8. 2003..Recent data suggests that a special class of ncRNAs called microRNAs might be involved in human disease. This review proposes a role for microRNAs in oncogenesis...
- Gene silencing in mammals by small interfering RNAsMichael T McManus
Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street E17 526, Cambridge, Massachusetts 02139, USA
Nat Rev Genet 3:737-47. 2002..Although the use of siRNAs to silence genes in vertebrate cells was only reported a year ago, the emerging literature indicates that most vertebrate genes can be studied with this technology...
- A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interferenceDouglas A Rubinson
Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
Nat Genet 33:401-6. 2003..They may also provide new approaches for gene therapy...
- Small interfering RNA-mediated gene silencing in T lymphocytesMichael T McManus
Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street E17 526, Cambridge, MA 02139, USA
J Immunol 169:5754-60. 2002..Using primary differentiated T lymphocytes, we provide the first evidence of siRNA-mediated RNAi gene silencing in normal nontransformed somatic mammalian lymphocytes...
- RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcriptionQing Ge
Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 100:2718-23. 2003..The findings reveal that newly synthesized NP and PA proteins are required for influenza virus transcription and replication and provide a basis for the development of siRNAs as prophylaxis and therapy for influenza infection in humans...
- Dicer1 and miR-219 Are required for normal oligodendrocyte differentiation and myelinationJason C Dugas
Department of Neurobiology, Stanford University School of Medicine, Stanford, CA 94305 5125, USA
Neuron 65:597-611. 2010..Together, these findings show that miR-219 plays a critical role in coupling differentiation to proliferation arrest in the OL lineage, enabling the rapid transition from proliferating OPCs to myelinating OLs...
- TAZ, a transcriptional modulator of mesenchymal stem cell differentiationJeong Ho Hong
Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
Science 309:1074-8. 2005..These results indicate that TAZ functions as a molecular rheostat that modulates MSC differentiation...
- Lentiviral strategies for RNAi knockdown of neuronal genesJill R Crittenden
Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Curr Protoc Neurosci . 2007..Additional protocols describe production and titering of the lentivirus, as well as safety testing. Finally, methods are provided for infecting neurons in culture and in vivo with RNAi lentivirus...
- Gene silencing using micro-RNA designed hairpinsMichael T McManus
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA
RNA 8:842-50. 2002..Furthermore, these RNAs are active when expressed by DNA vectors containing polymerase III promoters, opening the possibility for new approaches in stable RNAi-based loss of function studies...
- The RNaseIII enzyme Dicer is required for morphogenesis but not patterning of the vertebrate limbBrian D Harfe
Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA
Proc Natl Acad Sci U S A 102:10898-903. 2005..Thus, Dicer is required for the formation of normal mouse limbs. Strikingly, however, we did not detect defects in basic patterning or in tissue-specific differentiation of Dicer-deficient limb buds...
- Podocyte-specific deletion of dicer alters cytoskeletal dynamics and causes glomerular diseaseScott J Harvey
Renal Division, Washington University School of Medicine, St Louis, MO 63110, USA
J Am Soc Nephrol 19:2150-8. 2008..The unique architecture of podocytes may make them especially susceptible to cytoskeletal alterations initiated by aberrant miRNA dynamics...
- Selective miRNA disruption in T reg cells leads to uncontrolled autoimmunityXuyu Zhou
Diabetes Center, University of California, San Francisco, San Francisco, CA 94143, USA
J Exp Med 205:1983-91. 2008..These results support a central role for miRNAs in maintaining the stability of differentiated T reg cell function in vivo and homeostasis of the adaptive immune system...
- Conditional loss of Dicer disrupts cellular and tissue morphogenesis in the cortex and hippocampusTigwa H Davis
Department of Ophthalmology, University of California, San Francisco, San Francisco, California 94143 0730, USA
J Neurosci 28:4322-30. 2008....
- MicroRNA-responsive 'sensor' transgenes uncover Hox-like and other developmentally regulated patterns of vertebrate microRNA expressionJennifer H Mansfield
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA
Nat Genet 36:1079-83. 2004..Furthermore, miR-196a negatively regulates Hoxb8, indicating that its restricted expression pattern probably reflects a role in the patterning function of the Hox complex...
- Michael T. McManus: Interrupting biology [interview by Hema Bashyam]Michael T McManus
J Exp Med 205:506-7. 2008
- Unintentional miRNA ablation is a risk factor in gene knockout studies: a short reportIvan Osokine
Department of Microbiology and Immunology, University of California San Francisco Diabetes Center, University of California San Francisco, San Francisco, California, United States of America
PLoS Genet 4:e34. 2008..These data also raise the possibility that many knockout studies may need to be reexamined to determine if loss of a microRNA contributes to the phenotypic consequences attributed to loss of a protein-encoding gene...
- Behind the scenes of a small RNA gene-silencing pathwayGregory Ku
Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Francisco, San Francisco, CA 94122, USA
Hum Gene Ther 19:17-26. 2008..This review discusses advances in the biogenesis of miRNAs, their modes of action, and implications for RNA-based treatments...
- Essential role for Dicer during skeletal muscle developmentJason R O'Rourke
Department of Molecular Genetics and Microbiology and the Genetics Institute, University of Florida, College of Medicine, Gainesville, FL 32610 0266, USA
Dev Biol 311:359-68. 2007..These observations demonstrate key roles for Dicer in skeletal muscle and implicate miRNAs as critical components required for embryonic myogenesis...
- MicroRNA expression is required for pancreatic islet cell genesis in the mouseFrancis C Lynn
Diabetes Center, Hormone Research Institute, University of California San Francisco, 513 Parnassus Ave, San Francisco, CA 94143 0534, USA
Diabetes 56:2938-45. 2007..We tested the hypothesis that microRNAs (miRNAs), which limit gene expression through posttranscriptional silencing, modulate the gene expression cascades involved in pancreas development...
- Dysregulation of cardiogenesis, cardiac conduction, and cell cycle in mice lacking miRNA-1-2Yong Zhao
Gladstone Institute of Cardiovascular Disease, 1650 Owens Street, San Francisco, CA 94158, USA
Cell 129:303-17. 2007..These findings indicate that subtle alteration of miRNA dosage can have profound consequences in mammals and demonstrate the utility of mammalian loss-of-function models in revealing physiologic miRNA targets...
- Small RNAs and immunityMichael T McManus
UCSF Diabetes Center, Department of Microbiology and Immunology, University of California, San Francisco, CA 94122, USA
Immunity 21:747-56. 2004..This review discusses the potential roles of small RNAs in immune biology and speculates on their potential participation in lymphogenesis and antiviral mechanisms...
- The microRNA miR-196 acts upstream of Hoxb8 and Shh in limb developmentEran Hornstein
Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston, Massachusetts 02115, USA
Nature 438:671-4. 2005....
- Slowing down the Ras lane: miRNAs as tumor suppressors?John P Morris
UCSF Diabetes Center, Department of Microbiology and Immunology, University of California, San Francisco, CA 94122 0534, USA
Sci STKE 2005:pe41. 2005....
- MicroRNAs and endocrine biologyTrinna L Cuellar
UCSF Diabetes Center, Department of Microbiology and Immunology, University of California, San Francisco, California 94122 0534, USA
J Endocrinol 187:327-32. 2005..This review provides a general background regarding current knowledge about miRNA biogenesis and the potential contributions of these RNAs to endocrine function...