Timothy K Lu

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. pmc Tunable and multifunctional eukaryotic transcription factors based on CRISPR/Cas
    Fahim Farzadfard
    Department of Electrical Engineering and Computer Science and Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
    ACS Synth Biol 2:604-13. 2013
  2. pmc Next-generation synthetic gene networks
    Timothy K Lu
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Biotechnol 27:1139-50. 2009
  3. pmc Engineering scalable biological systems
    Timothy K Lu
    Synthetic Biology Group, Research Lab of Electronics, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
    Bioeng Bugs 1:378-84. 2010
  4. doi request reprint The next generation of bacteriophage therapy
    Timothy K Lu
    Synthetic Biology Group, Research Laboratory of Electronics, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
    Curr Opin Microbiol 14:524-31. 2011
  5. pmc Synthesis and patterning of tunable multiscale materials with engineered cells
    Allen Y Chen
    1 Biophysics Program, Harvard University, Cambridge, Massachusetts 02138, USA 2 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, USA 3 Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, USA 4 MIT Synthetic Biology Center, 500 Technology Square Cambridge, Massachusetts 02139, USA 5 Harvard MIT Health Sciences and Technology, Institute for Medical Engineering and Science, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, USA
    Nat Mater 13:515-23. 2014
  6. pmc Engineered bacteriophage targeting gene networks as adjuvants for antibiotic therapy
    Timothy K Lu
    Harvard Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 106:4629-34. 2009
  7. doi request reprint Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM
    Alan S L Wong
    Synthetic Biology Group, Massachusetts Institute of Technology MIT Synthetic Biology Center, MIT, Cambridge, MA 02139 Research Laboratory of Electronics, MIT, Cambridge, MA 02139
    Proc Natl Acad Sci U S A 113:2544-9. 2016
  8. pmc Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases
    Robert J Citorik
    1 MIT Microbiology Program, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA 2 MIT Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Biotechnol 32:1141-5. 2014
  9. pmc Strong underwater adhesives made by self-assembling multi-protein nanofibres
    Chao Zhong
    1 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA 2 Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA 3 Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA
    Nat Nanotechnol 9:858-66. 2014
  10. doi request reprint Engineering genetic circuits that compute and remember
    Piro Siuti
    1 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA 2 Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA 3 Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA 4
    Nat Protoc 9:1292-300. 2014

Collaborators

  • Zhengtao Deng
  • Ernest Fraenkel
  • Collin M Stultz
  • Rahul Sarpeshkar
  • Omid C Farokhzad
  • Allen A Cheng
  • Bijan Zakeri
  • Robert J Citorik
  • Alan S L Wong
  • Samuel D Perli
  • Piro Siuti
  • Barbara Jusiak
  • Kevin C Ma
  • Gigi C G Choi
  • Pablo Perez-Pinera
  • Lior Nissim
  • Allen Y Chen
  • Mark Mimee
  • Chao Zhong
  • John Yazbek
  • Fahim Farzadfard
  • Ramiz Daniel
  • Aleksandar F Radovic-Moreno
  • Sara Cleto
  • Mario Hermann
  • Pamela Milani
  • Cheryl H Cui
  • Aleth Gaillard
  • Alex K Shalek
  • Miriam Adam
  • Gabriela Pregernig
  • Samuel W Kazer
  • Oliver Purcell
  • Jordan Downey
  • Amanda N Billings
  • Huiming Ding
  • Michelle Y Lu
  • Thomas Gurry
  • Urartu O S Seker
  • Alexandra Fridkin
  • Jacob R Rubens
  • Robert Langer
  • Vlad A Puscasu
  • Christopher J Yoon

Detail Information

Publications23

  1. pmc Tunable and multifunctional eukaryotic transcription factors based on CRISPR/Cas
    Fahim Farzadfard
    Department of Electrical Engineering and Computer Science and Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
    ACS Synth Biol 2:604-13. 2013
    ....
  2. pmc Next-generation synthetic gene networks
    Timothy K Lu
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Biotechnol 27:1139-50. 2009
    ..As these challenges are addressed, synthetic biologists will be able to construct useful next-generation synthetic gene networks with real-world applications in medicine, biotechnology, bioremediation and bioenergy...
  3. pmc Engineering scalable biological systems
    Timothy K Lu
    Synthetic Biology Group, Research Lab of Electronics, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
    Bioeng Bugs 1:378-84. 2010
    ....
  4. doi request reprint The next generation of bacteriophage therapy
    Timothy K Lu
    Synthetic Biology Group, Research Laboratory of Electronics, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
    Curr Opin Microbiol 14:524-31. 2011
    ..These research efforts must be coupled with practical and rigorous approaches at academic, commercial, and regulatory levels in order to successfully advance bacteriophage therapy into clinical settings...
  5. pmc Synthesis and patterning of tunable multiscale materials with engineered cells
    Allen Y Chen
    1 Biophysics Program, Harvard University, Cambridge, Massachusetts 02138, USA 2 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, USA 3 Department of Biological Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, USA 4 MIT Synthetic Biology Center, 500 Technology Square Cambridge, Massachusetts 02139, USA 5 Harvard MIT Health Sciences and Technology, Institute for Medical Engineering and Science, 77 Massachusetts Avenue Cambridge, Massachusetts 02139, USA
    Nat Mater 13:515-23. 2014
    ..This work lays a foundation for synthesizing, patterning, and controlling functional composite materials with engineered cells...
  6. pmc Engineered bacteriophage targeting gene networks as adjuvants for antibiotic therapy
    Timothy K Lu
    Harvard Massachusetts Institute of Technology Division of Health Sciences and Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 106:4629-34. 2009
    ..This work establishes a synthetic biology platform for the rapid translation and integration of identified targets into effective antibiotic adjuvants...
  7. doi request reprint Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM
    Alan S L Wong
    Synthetic Biology Group, Massachusetts Institute of Technology MIT Synthetic Biology Center, MIT, Cambridge, MA 02139 Research Laboratory of Electronics, MIT, Cambridge, MA 02139
    Proc Natl Acad Sci U S A 113:2544-9. 2016
    ....
  8. pmc Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases
    Robert J Citorik
    1 MIT Microbiology Program, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA 2 MIT Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Biotechnol 32:1141-5. 2014
    ....
  9. pmc Strong underwater adhesives made by self-assembling multi-protein nanofibres
    Chao Zhong
    1 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA 2 Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA 3 Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA
    Nat Nanotechnol 9:858-66. 2014
    ..Moreover, they outperform Mfps or curli fibres taken on their own and exhibit better tolerance to auto-oxidation than Mfps at pH ≥ 7.0. ..
  10. doi request reprint Engineering genetic circuits that compute and remember
    Piro Siuti
    1 Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA 2 Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA 3 Synthetic Biology Group, Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA 4
    Nat Protoc 9:1292-300. 2014
    ..It should enable the encoding of advanced computational operations in living cells, including sequential-logic and biological-state machines, for a broad range of applications in biotechnology, basic science and biosensing. ..
  11. pmc Synthetic biology of antimicrobial discovery
    Bijan Zakeri
    Synthetic Biology Group, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States
    ACS Synth Biol 2:358-72. 2013
    ..Herein, we discuss the biological engineering of small molecules, peptides, and non-traditional antimicrobials and provide an overview of the growing applicability of synthetic biology to antimicrobials discovery. ..
  12. doi request reprint Synthetic circuits integrating logic and memory in living cells
    Piro Siuti
    Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Biotechnol 31:448-52. 2013
    ..We envision that this integrated logic and memory system will enable the implementation of complex cellular state machines, behaviors and pathways for therapeutic, diagnostic and basic science applications...
  13. doi request reprint Synthetic biology: an emerging engineering discipline
    Allen A Cheng
    Synthetic Biology Group, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Annu Rev Biomed Eng 14:155-78. 2012
    ..This review aims to discuss the progress and challenges in synthetic biology and to illustrate areas where synthetic biology may impact biomedical engineering and human health...
  14. pmc Surface charge-switching polymeric nanoparticles for bacterial cell wall-targeted delivery of antibiotics
    Aleksandar F Radovic-Moreno
    Harvard MIT Division of Health Sciences and Technology, Cambridge, Massachusetts 02139, USA
    ACS Nano 6:4279-87. 2012
    ..The PLGA-PLH-PEG NPs described herein are a first step toward developing systemically administered drug carriers that can target and potentially treat Gram-positive, Gram-negative, or polymicrobial infections associated with acidity...
  15. doi request reprint Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology
    Barbara Jusiak
    Research Laboratory of Electronics, Synthetic Biology Center, Department of Biological Engineering and Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
    Trends Biotechnol . 2016
    ....
  16. doi request reprint Massively parallel high-order combinatorial genetics in human cells
    Alan S L Wong
    Synthetic Biology Group, MIT Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    Nat Biotechnol 33:952-61. 2015
    ..More broadly, our method will enable high-throughput profiling of multifactorial genetic combinations that regulate phenotypes of relevance to biomedicine, biotechnology and basic science. ..
  17. doi request reprint DNA nanotechnology: new adventures for an old warhorse
    Bijan Zakeri
    Department of Electrical Engineering and Computer Science, Department of Biological Engineering, Research Laboratory of Electronics, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA MIT Synthetic Biology Center, 500 Technology Square, Cambridge, MA 02139, USA Electronic address
    Curr Opin Chem Biol 28:9-14. 2015
    ..Early studies of DNA as an alternative to magnetic and optical storage mediums have not only been promising, but have demonstrated the potential of DNA to revolutionize the way we interact with digital data in the future. ..
  18. doi request reprint Foundations and Emerging Paradigms for Computing in Living Cells
    Kevin C Ma
    Synthetic Biology Group, Research Laboratory of Electronics, Department of Biological Engineering and Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    J Mol Biol 428:893-915. 2016
    ..Finally, we discuss notable challenges that the field of synthetic biology still faces in achieving reliable and predictable forward-engineering of artificial biological circuits. ..
  19. pmc Enhanced killing of antibiotic-resistant bacteria enabled by massively parallel combinatorial genetics
    Allen A Cheng
    Synthetic Biology Group, MIT Synthetic Biology Center, Research Lab of Electronics, Departments of Electrical Engineering and Computer Science and Biological Engineering, Medical Engineering and Medical Physics Program, Harvard MIT Health Sciences and Technology, Harvard Biophysics Program and Harvard MD PhD Program, Harvard University, Boston, MA 02115
    Proc Natl Acad Sci U S A 111:12462-7. 2014
    ....
  20. pmc Bacteriophage-based synthetic biology for the study of infectious diseases
    Robert J Citorik
    MIT Microbiology Program, 77 Massachusetts Avenue, Cambridge, MA 02139, USA MIT Synthetic Biology Center, 500 Technology Square, Cambridge, MA 02139, USA
    Curr Opin Microbiol 19:59-69. 2014
    ..Though almost a century has elapsed since their discovery, bacteriophages continue to have a major impact on modern biological sciences, especially with the growth of multidrug-resistant bacteria and interest in the microbiome. ..
  21. pmc Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells
    Lior Nissim
    Synthetic Biology Group, Research Laboratory of Electronics, Department of Biological Engineering and Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Mol Cell 54:698-710. 2014
    ..This toolkit can be used for programming scalable gene circuits and perturbing endogenous networks for biology, therapeutic, and synthetic biology applications. ..
  22. doi request reprint Synthetic analog computation in living cells
    Ramiz Daniel
    Analog Circuits and Biological Systems Group, Research Lab of Electronics, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 497:619-23. 2013
    ..Such circuits may lead to new applications for synthetic biology and biotechnology that require complex computations with limited parts, need wide-dynamic-range biosensing or would benefit from the fine control of gene expression...
  23. pmc Dispersing biofilms with engineered enzymatic bacteriophage
    Timothy K Lu
    Harvard MIT Division of Health Sciences and Technology, 77 Massachusetts Avenue, Room E25 519, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 104:11197-202. 2007
    ..This work demonstrates the feasibility and benefits of using engineered enzymatic bacteriophage to reduce bacterial biofilms and the applicability of synthetic biology to an important medical and industrial problem...