Research Topics
Species | Aleksey LomakinSummaryAffiliation: Massachusetts Institute of Technology Country: USA Publications
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Publications
Quasielastic light scattering study of amyloid beta-protein fibril formationAleksey Lomakin
Department of Physics and Center for Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Protein Pept Lett 13:247-54. 2006..We illustrate practical applications of QLS with examples from studies of fibril formation of the amyloid beta-protein...
Quasielastic light scattering study of amyloid β-protein fibrillogenesisAleksey Lomakin
Materials Processing Center, Massachusetts Institute of Technology, Cambridge, MA, USA
Methods Mol Biol 849:69-83. 2012..We illustrate here how QLS has been applied to elucidate features of the oligomerization and fibrillogenesis of the amyloid β-protein, Aβ, thought to be the causative agent of Alzheimer's disease...
Phase behavior of mixtures of human lens proteins Gamma D and Beta B1Ying Wang
Materials Processing Center, Department of Physics, and Center for Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 107:13282-7. 2010..Our findings provide insight into the factors essential for maintaining lens proteins in a single homogeneous phase, thereby enabling lens transparency...
Altered phase diagram due to a single point mutation in human gammaD-crystallinJennifer J McManus
Materials Processing Center, Department of Physics, and Center for Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 104:16856-61. 2007....
Pathological crystallization of human immunoglobulinsYing Wang
Materials Processing Center, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 109:13359-61. 2012..Remarkably, the crystallization can occur at quite low concentrations. This suggests that, even within the regular immune response to infections, cryoprecipitation of Ig can be possible...
Thickness-radius relationship and spring constants of cholesterol helical ribbonsBoris Khaykovich
Nuclear Reactor Laboratory, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 106:15663-6. 2009....
Quasielastic light scattering for protein assembly studiesAleksey Lomakin
Department of Physics and Center for Materials Sciences and Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA
Methods Mol Biol 299:153-74. 2005..We use examples from studies of Abeta fibrillogenesis to illustrate QLS application for understanding the molecular mechanisms of the nucleation and growth of amyloid fibrils...
Phase separation in solutions of monoclonal antibodies and the effect of human serum albuminYing Wang
Materials Processing Center, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 108:16606-11. 2011..Our findings are relevant to understanding the stability of pharmaceutical solutions of antibodies and the mechanisms of cryoglobulinemia...
Structure determination of micelle-like intermediates in amyloid beta -protein fibril assembly by using small angle neutron scatteringWinnie Yong
Department of Physics, Center for Materials Science and Engineering, and Materials Processing Center, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
Proc Natl Acad Sci U S A 99:150-4. 2002..The concentration independence of the length of the cylindrical aggregate indicates the presence of an internal nonrepetitive structure that spans the entire length of the Abeta assembly...
Liquid-solid transition in nuclei of protein crystalsAleksey Lomakin
Department of Physics, Center for Materials Science and Engineering and Material Processing Center, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA
Proc Natl Acad Sci U S A 100:10254-7. 2003..A nucleus first forms and grows as a disordered, liquid-like aggregate. Once the aggregate grows beyond a critical size (about a few hundred particles) crystal nucleation becomes possible...
Structure of cholesterol helical ribbons and self-assembling biological springsBoris Khaykovich
Nuclear Reactor Laboratory, Massachusetts Institute of Technology, 138 Albany Street, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 104:9656-60. 2007..We discuss possible origins for this triplication as well as the connection between the crystalline structure and the geometrical form of the helical ribbons...
Effect of polyethylene glycol on the liquid-liquid phase transition in aqueous protein solutionsOnofrio Annunziata
Department of Physics, Center for Materials Science and Engineering, and Materials Processing Center, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA
Proc Natl Acad Sci U S A 99:14165-70. 2002..Finally, we show that the increase of the LLPS temperature with PEG concentration is due to attractive protein-protein interactions...
Familial Alzheimer's disease mutations alter the stability of the amyloid beta-protein monomer folding nucleusMarianne A Grant
Division of Molecular and Vascular Medicine, Beth Israel Deaconess Medical Center, and Department of Medicine, Harvard Medical School, Boston, MA 02215, USA
Proc Natl Acad Sci U S A 104:16522-7. 2007..The implications of these findings for understanding Abeta monomer folding and disease causation are discussed...
C-terminal peptides coassemble into Abeta42 oligomers and protect neurons against Abeta42-induced neurotoxicityErica A Fradinger
Department of Neurology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095, USA
Proc Natl Acad Sci U S A 105:14175-80. 2008..Thus, Abeta(31-42) and Abeta(39-42) are leads for obtaining mechanism-based drugs for treatment of AD using a systematic structure-activity approach...
Amyloid beta -protein (Abeta) assembly: Abeta 40 and Abeta 42 oligomerize through distinct pathwaysGal Bitan
Center for Neurologic Diseases, Brigham and Women's Hospital, Boston, MA 02115, USA
Proc Natl Acad Sci U S A 100:330-5. 2003..The strong etiologic association of Abeta42 with AD may thus be a result of assemblies formed at the earliest stages of peptide oligomerization...
