Research Topics
Genomes and GenesSpecies | Christopher J LengnerSummaryAffiliation: Massachusetts Institute of Technology Country: USA Publications
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Publications
The pluripotency regulator Oct4: a role in somatic stem cells?Christopher J Lengner
The Whitehead Institute for Biomedical Research, Cambridge, Massachussets 01242, USA
Cell Cycle 7:725-8. 2008..Here we contrast pathways known to govern pluripotency in embryonic stem cells with those in adult stem cells and critically discuss the concept of pluripotency in adult stem cells of the mammalian soma...
iPS cell technology in regenerative medicineChristopher J Lengner
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA
Ann N Y Acad Sci 1192:38-44. 2010..Here we discuss how advances in iPS cell technology have led to the generation of patient-specific cell lines that can potentially be used to model human diseases and ultimately act as therapeutic agents...
Transgenic mice with defined combinations of drug-inducible reprogramming factorsStyliani Markoulaki
The Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
Nat Biotechnol 27:169-71. 2009..This approach creates a defined system for studying reprogramming mechanisms and allows screening of genetically homogeneous cells for compounds that can replace any transcription factor required for iPS cell derivation...- Metastable pluripotent states in NOD-mouse-derived ESCsJacob Hanna
The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
Cell Stem Cell 4:513-24. 2009..Our findings suggest that stem cells from different genetic backgrounds can assume distinct states of pluripotency in vitro, the stability of which is regulated by endogenous genetic determinants and can be modified by exogenous factors... - A drug-inducible transgenic system for direct reprogramming of multiple somatic cell typesMarius Wernig
Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, Massachusetts 02142, USA
Nat Biotechnol 26:916-24. 2008..This system facilitates the characterization of reprogramming and provides a tool for genetic or chemical screens to enhance reprogramming... - Reprogramming of postnatal neurons into induced pluripotent stem cells by defined factorsJongpil Kim
Whitehead Institute for Biomedical Research and, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Stem Cells 29:992-1000. 2011.... - Functional integration of dopaminergic neurons directly converted from mouse fibroblastsJongpil Kim
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
Cell Stem Cell 9:413-9. 2011..Thus, iDA neurons generated from abundant somatic fibroblasts by direct lineage reprogramming hold promise for modeling neurodegenerative disease and for cell-based therapies of PD... - Direct cell reprogramming is a stochastic process amenable to accelerationJacob Hanna
The Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA
Nature 462:595-601. 2009.... - Human embryonic stem cells with biological and epigenetic characteristics similar to those of mouse ESCsJacob Hanna
The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
Proc Natl Acad Sci U S A 107:9222-7. 2010..The generation of validated "naïve" human ESCs will allow the molecular dissection of a previously undefined pluripotent state in humans and may open up new opportunities for patient-specific, disease-relevant research... - Direct reprogramming of terminally differentiated mature B lymphocytes to pluripotencyJacob Hanna
The Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
Cell 133:250-64. 2008..Our study provides definite proof for the direct nuclear reprogramming of terminally differentiated adult cells to pluripotency... - Derivation of pre-X inactivation human embryonic stem cells under physiological oxygen concentrationsChristopher J Lengner
Whitehead Institute for Biomedical Sciences, 9 Cambridge Center, Cambridge, MA 02142, USA
Cell 141:872-83. 2010..These findings indicate that the human blastocyst contains pre-X-inactivation cells and that this state is preserved in vitro through culture under physiological oxygen... - Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cellsTobias Brambrink
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142, USA
Cell Stem Cell 2:151-9. 2008..Importantly, the virally transduced cDNAs needed to be expressed for at least 12 days in order to generate iPS cells. Our results are a step toward understanding some of the molecular events governing epigenetic reprogramming... - Oct4 expression is not required for mouse somatic stem cell self-renewalChristopher J Lengner
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, MA 02142, USA
Cell Stem Cell 1:403-15. 2007..Oct4 gene ablation in these tissues revealed no abnormalities in homeostasis or regenerative capacity. We conclude that Oct4 is dispensable for both self-renewal and maintenance of somatic stem cells in the adult mammal...
Research Grants
- Control of Somatic Stem Cell Proliferation by Oct4Christopher Lengner; Fiscal Year: 2007..This proposal will delineate molecular mechanisms that can potentially be manipulated to enhance regenerative medicine. ..