Alexander Klibanov

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. ncbi request reprint Why are enzymes less active in organic solvents than in water?
    A M Klibanov
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA
    Trends Biotechnol 15:97-101. 1997
  2. ncbi request reprint On the relationship between conformation and stability in solid pharmaceutical protein formulations
    Alexander M Klibanov
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biotechnol Lett 26:1103-6. 2004
  3. ncbi request reprint Non-viral siRNA delivery to the lung
    Mini Thomas
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Adv Drug Deliv Rev 59:124-33. 2007
  4. pmc Highly effective gene transfection in vivo by alkylated polyethylenimine
    Jennifer A Fortune
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Drug Deliv 2011:204058. 2011
  5. ncbi request reprint Asymmetric enzymatic oxidoreductions in organic solvents
    Alexander M Klibanov
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Curr Opin Biotechnol 14:427-31. 2003
  6. ncbi request reprint Deterioration of lyophilized pharmaceutical proteins
    H R Costantino
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biochemistry (Mosc) 63:357-63. 1998
  7. ncbi request reprint Improving enzymes by using them in organic solvents
    A M Klibanov
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA
    Nature 409:241-6. 2001
  8. ncbi request reprint A method to investigate non-thermal effects of radio frequency radiation on pharmaceuticals with relevance to RFID technology
    Felicia C A I Cox
    Dept of Electr Eng, MIT, Cambridge, MA 02139, USA
    Conf Proc IEEE Eng Med Biol Soc 1:4340-3. 2006
  9. ncbi request reprint Co-lyophilization with D-proline greatly enhances peroxidase's stereoselectivity in a non-aqueous medium
    Ju Hyun Yu
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, 02139, USA
    Biotechnol Lett 28:555-8. 2006
  10. ncbi request reprint Binding of hydrophobic hydroxamic acids enhances peroxidase's stereoselectivity in nonaqueous sulfoxidations
    Prasanta Kumar Das
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 124:782-7. 2002

Research Grants

Collaborators

  • Jennifer Fiegel
  • H R Costantino
  • Qing Li
  • Felicia C A I Cox
  • Jun Wang
  • ROBERT SAMUEL LANGER
  • Qing Ge
  • Yuan Lin
  • Mini Thomas
  • Eugene Antipov
  • Vikas K Sharma
  • Jianzhu Chen
  • James J Lu
  • Prasanta Kumar Das
  • Jennifer A Fortune
  • Sze Yinn Wong
  • Art E Cho
  • Jose M M Caaveiro
  • K Dane Wittrup
  • Antonia M Rojas
  • Wiwik Watanabe
  • Dasa Lipovsek
  • Ju Hyun Yu
  • Nebojsa M Milovic
  • Sergei V Dzyuba
  • Jian Lin
  • Susana Luque
  • Yuchun Xie
  • Tatiana I Novobrantseva
  • Byeong Su Kim
  • Paula T Hammond
  • Jovana Veselinovic
  • Robert Clarke
  • Matthew Thomas
  • Kathryn A Armstrong
  • Bruce Tidor
  • Jeffrey Katstra
  • Mark J Olsen
  • Lester C Griel
  • David Edwards
  • Gerald G Fuller
  • Pedro A Gonzalez
  • Chengcheng Zhang
  • Kim Lewis
  • Karen K Gleason
  • Bradley D Olsen
  • Shashi K Murthy

Detail Information

Publications26

  1. ncbi request reprint Why are enzymes less active in organic solvents than in water?
    A M Klibanov
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA
    Trends Biotechnol 15:97-101. 1997
    ....
  2. ncbi request reprint On the relationship between conformation and stability in solid pharmaceutical protein formulations
    Alexander M Klibanov
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biotechnol Lett 26:1103-6. 2004
    ..This phenomenon may profoundly affect deleterious processes in lyophilized proteins, e.g. moisture-induced aggregation, as illustrated in this review with bovine serum and recombinant human albumins...
  3. ncbi request reprint Non-viral siRNA delivery to the lung
    Mini Thomas
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Adv Drug Deliv Rev 59:124-33. 2007
    ..This review focuses on the recent progress in non-viral delivery of siRNAs for the treatment of lung diseases, particularly infectious diseases. The rapid progress will put siRNA-based therapeutics on fast track to the clinic...
  4. pmc Highly effective gene transfection in vivo by alkylated polyethylenimine
    Jennifer A Fortune
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Drug Deliv 2011:204058. 2011
    ..As these enhancements in gene expression occur without changing the plasmid biodistribution, alkylation does not alter the cellular uptake but rather enhances transfection subsequent to cellular uptake...
  5. ncbi request reprint Asymmetric enzymatic oxidoreductions in organic solvents
    Alexander M Klibanov
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Curr Opin Biotechnol 14:427-31. 2003
    ..Consequently, novel synthetic and biotechnological opportunities ensue, as illustrated herein by those based on enzymatic oxidoreductions such as the asymmetric peroxidase-catalyzed sulfoxidation of organic sulfides...
  6. ncbi request reprint Deterioration of lyophilized pharmaceutical proteins
    H R Costantino
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biochemistry (Mosc) 63:357-63. 1998
    ..It is illustrated how knowledge of such deterioration pathways makes possible the rational development of stable solid protein formulations...
  7. ncbi request reprint Improving enzymes by using them in organic solvents
    A M Klibanov
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA
    Nature 409:241-6. 2001
    ..Enzyme-catalysed reactions in organic solvents, and even in supercritical fluids and the gas phase, have found numerous potential applications, some of which are already commercialized...
  8. ncbi request reprint A method to investigate non-thermal effects of radio frequency radiation on pharmaceuticals with relevance to RFID technology
    Felicia C A I Cox
    Dept of Electr Eng, MIT, Cambridge, MA 02139, USA
    Conf Proc IEEE Eng Med Biol Soc 1:4340-3. 2006
    ..This methodology provides a well-controlled approach to investigate the non-thermal effects of RFR for a range of incident power intensities and frequencies and initial sample temperatures...
  9. ncbi request reprint Co-lyophilization with D-proline greatly enhances peroxidase's stereoselectivity in a non-aqueous medium
    Ju Hyun Yu
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, 02139, USA
    Biotechnol Lett 28:555-8. 2006
    ..While many improved the stereoselectivity of (and significantly activated) HRP, the greatest effect was observed with D-proline which enhanced the E(S/R) value by over an order of magnitude from synthetically meaningless to useful...
  10. ncbi request reprint Binding of hydrophobic hydroxamic acids enhances peroxidase's stereoselectivity in nonaqueous sulfoxidations
    Prasanta Kumar Das
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 124:782-7. 2002
    ..The generality of the observed ligand-induced stereoselectivity enhancement is demonstrated with other hydrophobic hydroxamic acids, as well as with additional thioether substrates...
  11. ncbi request reprint Asymmetric thiosulfinations catalyzed by bovine serum albumin and horseradish peroxidase
    Sergei V Dzyuba
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biotechnol Lett 25:1961-5. 2003
    ..The rates of HRP-catalyzed thiosulfination in methanol were several times faster than in water but the enzyme was less stereoselective...
  12. ncbi request reprint Moisture-induced aggregation of lyophilized DNA and its prevention
    Vikas K Sharma
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Pharm Res 24:168-75. 2007
    ..To investigate the moisture-induced aggregation (i.e., a loss of solubility in water) of DNA in a solid state and to develop rational strategies for its prevention...
  13. pmc Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method
    Eugene Antipov
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 105:17694-9. 2008
    ..These marked improvements in enantioselectivity are demonstrated for the surface-bound and soluble enzymes and rationalized by computational docking studies...
  14. pmc How a single-point mutation in horseradish peroxidase markedly enhances enantioselectivity
    Eugene Antipov
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 131:11155-60. 2009
    ....
  15. ncbi request reprint Asymmetric sulfoxidations mediated by alpha-chymotrypsin
    Prasanta Kumar Das
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biotechnol Bioeng 78:104-9. 2002
    ..The stereoselectivity of the chymotrypsin-mediated sulfoxidations is correctly explained by means of structure-based molecular modeling of the enzyme-sulfide complexes...
  16. ncbi request reprint Polycation-mediated delivery of siRNAs for prophylaxis and treatment of influenza virus infection
    Mini Thomas
    Division of Biological Engineering, Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Expert Opin Biol Ther 5:495-505. 2005
    ....
  17. ncbi request reprint Specificity of a DNA-based (DNAzyme) peroxidative biocatalyst
    Antonia M Rojas
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biotechnol Lett 29:227-32. 2007
    ..Herein substrate, regio-, enantiomeric, and diastereomeric selectivities of heme, the DNAzyme, and the enzyme horseradish peroxidase are comparatively examined...
  18. ncbi request reprint Why inhaling salt water changes what we exhale
    Wiwik Watanabe
    Pulmatrix Inc, 12 Emily Street, Cambridge, MA 02139, USA
    J Colloid Interface Sci 307:71-8. 2007
    ..We confirm these effects in vivo and show their reversibility through nebulization of saline solutions to anesthetized bull calves...
  19. pmc Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung
    Mini Thomas
    Department of Chemistry and Division of Biological Engineering, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 102:5679-84. 2005
    ..Furthermore, a polyplex of a siRNA against the influenza viral nucleocapsid protein gene and PEI87 resulted in a 94% drop of virus titers in the lungs of influenza-infected animals...
  20. ncbi request reprint Unexpectedly enhanced stereoselectivity of peroxidase-catalyzed sulfoxidation in branched alcohols
    Yuchun Xie
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biotechnol Bioeng 79:105-11. 2002
    ....
  21. doi request reprint Bactericidal and virucidal ultrathin films assembled layer by layer from polycationic N-alkylated polyethylenimines and polyanions
    Sze Yinn Wong
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biomaterials 31:4079-87. 2010
    ....
  22. ncbi request reprint Making thin polymeric materials, including fabrics, microbicidal and also water-repellent
    Jian Lin
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biotechnol Lett 25:1661-5. 2003
    ....
  23. ncbi request reprint Immobilized N-alkylated polyethylenimine avidly kills bacteria by rupturing cell membranes with no resistance developed
    Nebojsa M Milovic
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biotechnol Bioeng 90:715-22. 2005
    ..Finally, the immobilized N-alkyl-PEI, while deadly to bacteria, is determined to be harmless to mammalian (monkey kidney) cells...
  24. ncbi request reprint Selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display
    Dasa Lipovsek
    Biological Engineering Division, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Chem Biol 14:1176-85. 2007
    ..In contrast, the library constructed by using error-prone PCR yielded no HRP variants with a significantly improved enantioselectivity...
  25. ncbi request reprint Mechanistic studies on aggregation of polyethylenimine-DNA complexes and its prevention
    Vikas K Sharma
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biotechnol Bioeng 90:614-20. 2005
    ..It is concluded that PEI-DNA complexes aggregate primarily due to hydrophobic interactions, while electrostatic attractions play little role...
  26. ncbi request reprint Cross-linked small polyethylenimines: while still nontoxic, deliver DNA efficiently to mammalian cells in vitro and in vivo
    Mini Thomas
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Pharm Res 22:373-80. 2005
    ....

Research Grants4

  1. SPECIFICITY OF ENZYMATIC PROTEIN CLEAVAGE IN GLYCEROL VS WATER
    Alexander Klibanov; Fiscal Year: 1999
    ..Results indicate that the exposure to NO leads to a structural modification of specific sulfated residues. The structural requirements and the modification are being probed with model compounds and stable isotope labeling. ..
  2. SPECIFICITY OF ENZYMATIC PROTEIN CLEAVAGE IN GLYCEROL VS WATER
    Alexander Klibanov; Fiscal Year: 2000
    ..Results indicate that the exposure to NO leads to a structural modification of specific sulfated residues. The structural requirements and the modification are being probed with model compounds and stable isotope labeling. ..
  3. SPECIFICITY OF ENZYMATIC PROTEIN CLEAVAGE IN GLYCEROL VS WATER
    Alexander Klibanov; Fiscal Year: 2001
    ..Results indicate that the exposure to NO leads to a structural modification of specific sulfated residues. The structural requirements and the modification are being probed with model compounds and stable isotope labeling. ..