- Expression of recombinant proteins with uniform N-terminiOrsolya Kiraly
Department of Molecular and Cell Biology, Henry M Goldman School of Dental Medicine, Boston University, Boston, MA, USA
Methods Mol Biol 705:175-94. 2011..We describe a protocol using human cationic trypsinogen as an example to demonstrate that recombinant proteins produced in this expression system contain homogeneous, unprocessed N-termini...
- Expression of human cationic trypsinogen with an authentic N terminus using intein-mediated splicing in aminopeptidase P deficient Escherichia coliOrsolya Kiraly
Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA
Protein Expr Purif 48:104-11. 2006..The use of the novel pepP knock-out E. coli strain should be generally applicable to the expression of recombinant proteins, which undergo unwanted N-terminal trimming by aminopeptidase P...