Orsolya Kiraly

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. ncbi Expression of recombinant proteins with uniform N-termini
    Orsolya Kiraly
    Department of Molecular and Cell Biology, Henry M Goldman School of Dental Medicine, Boston University, Boston, MA, USA
    Methods Mol Biol 705:175-94. 2011
  2. ncbi Expression of human cationic trypsinogen with an authentic N terminus using intein-mediated splicing in aminopeptidase P deficient Escherichia coli
    Orsolya Kiraly
    Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA
    Protein Expr Purif 48:104-11. 2006

Detail Information

Publications2

  1. ncbi Expression of recombinant proteins with uniform N-termini
    Orsolya Kiraly
    Department of Molecular and Cell Biology, Henry M Goldman School of Dental Medicine, Boston University, Boston, MA, USA
    Methods Mol Biol 705:175-94. 2011
    ..We describe a protocol using human cationic trypsinogen as an example to demonstrate that recombinant proteins produced in this expression system contain homogeneous, unprocessed N-termini...
  2. ncbi Expression of human cationic trypsinogen with an authentic N terminus using intein-mediated splicing in aminopeptidase P deficient Escherichia coli
    Orsolya Kiraly
    Department of Molecular and Cell Biology, Goldman School of Dental Medicine, Boston University, Boston, MA 02118, USA
    Protein Expr Purif 48:104-11. 2006
    ..The use of the novel pepP knock-out E. coli strain should be generally applicable to the expression of recombinant proteins, which undergo unwanted N-terminal trimming by aminopeptidase P...