Affiliation: Massachusetts General Hospital
- Unraveling the links between diabetes, obesity, and cardiovascular diseasePaul L Huang
Circ Res 96:1129-31. 2005
- Role of endothelial nitric oxide in cerebrovascular regulationDmitriy N Atochin
Cardiovascular Research Center and Cardiology Division, Department of Medicine, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA
Curr Pharm Biotechnol 12:1334-42. 2011..Pharmacological and genetic manipulations of the endothelial NO system demonstrate the essential roles of endothelial NO synthase in maintenance of vascular tone and cerebral perfusion under normal and pathological conditions...
- eNOS, metabolic syndrome and cardiovascular diseasePaul L Huang
Cardiovascular Research Center and Cardiology Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
Trends Endocrinol Metab 20:295-302. 2009..This review focuses on endothelial nitric oxide synthase and summarizes evidence that a reduction in the bioavailability of endothelium-derived nitric oxide serves as a key link between metabolic disorders and cardiovascular risk...
- A comprehensive definition for metabolic syndromePaul L Huang
Cardiovascular Research Center, Massachusetts General Hospital, Charlestown, MA 02129, USA
Dis Model Mech 2:231-7. 2009..A comprehensive definition for the metabolic syndrome and its key features would facilitate research into its causes and hopefully lead to new insights into pharmacologic and lifestyle treatment approaches...
- Nitric oxide and cerebral ischemic preconditioningPaul L Huang
Harvard Medical School, Cardiology Division and Cardiovascular Research Center, Massachusetts General Hospital, 149 Thirteenth Street, Charlestown, MA 02129, USA
Cell Calcium 36:323-9. 2004..This article reviews the effects of NO following cerebral ischemia, the signaling pathways through which NO acts, and its potential roles in cerebral ischemic preconditioning...
- Cardiovascular roles of nitric oxide: a review of insights from nitric oxide synthase gene disrupted miceVictor W T Liu
Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, United States
Cardiovasc Res 77:19-29. 2008..Mice lacking the inducible isoform show reduced hypotension to septic shock. Together, NOS gene knockout mice have been useful tools that complement our other approaches to studying the multiple roles of NO in the cardiovascular system...
- Rapid cerebral ischemic preconditioning in mice deficient in endothelial and neuronal nitric oxide synthasesDmitriy N Atochin
Cardiology Division, Cardiovascular Research Center, Massachusetts General Hospital East, Charlestown, MA 02129, USA
Stroke 34:1299-303. 2003..The purpose of this study was to test the hypothesis that nitric oxide is required for preconditioning in an intact animal model of focal ischemia using neuronal and endothelial nitric oxide synthase (nNOS and eNOS) knockout mice...
- Role of endothelial nitric oxide synthase in endothelial activation: insights from eNOS knockout endothelial cellsPeter J Kuhlencordt
Cardiovascular Research Center, Massachusetts General Hospital East, 149 East 13th St, Charlestown, MA 02129, USA
Am J Physiol Cell Physiol 286:C1195-202. 2004..We propose that eNOS derived NO does not chronically suppress endothelial cell activation in an autocrine fashion but serves to counterbalance signals that mediate activation...
- Endothelial nitric oxide synthase regulates microlymphatic flow via collecting lymphaticsJeroen Hagendoorn
Department of Radiation Oncology, E L Steele Laboratory for Tumor Biology, Massachusetts General Hospital and Harvard Medical School, Boston, Mass 02114, USA
Circ Res 95:204-9. 2004..Our results provide the first in vivo evidence that eNOS affects function of the whole microlymphatic system and that it is regulated via the collecting lymphatics...
- Protective effects of statins involving both eNOS and tPA in focal cerebral ischemiaMinoru Asahi
Neuroprotection Research Laboratory, Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, 02129, USA
J Cereb Blood Flow Metab 25:722-9. 2005..These results suggest that statins protect against stroke by multiple mechanisms involving both eNOS and tPA. The involvement of each pathway may be revealed depending on the choice of experimental stroke model...
- NO mediates mural cell recruitment and vessel morphogenesis in murine melanomas and tissue-engineered blood vesselsSatoshi Kashiwagi
Edwin L Steele Laboratory, Department of Radiation Oncology, and Cardiovascular Research Center, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
J Clin Invest 115:1816-27. 2005..These data indicate that endothelial cell-derived NO induces mural cell recruitment as well as subsequent morphogenesis and stabilization of angiogenic vessels...
- Rho-kinase inhibition acutely augments blood flow in focal cerebral ischemia via endothelial mechanismsHwa Kyoung Shin
Department of Radiology, Stroke and Neurovascular Regulation Laboratory, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
J Cereb Blood Flow Metab 27:998-1009. 2007..Therefore, rapid nontranscriptional upregulation of eNOS activity by small molecule inhibitors of Rho-kinase may be a viable therapeutic approach in acute stroke...
- The phosphorylation state of eNOS modulates vascular reactivity and outcome of cerebral ischemia in vivoDmitriy N Atochin
Cardiovascular Research Center and Cardiology Division, Massachusetts General Hospital, Boston, MA 02129, USA
J Clin Invest 117:1961-7. 2007..They also demonstrate the in vivo relevance of posttranslational modification of eNOS in vascular function...
- Effects of neuroglobin overexpression on acute brain injury and long-term outcomes after focal cerebral ischemiaXiaoying Wang
Neuroprotection Research Laboratory, Departments of Neurology and Radiology, Massachusetts General Hospital, 149 13th Street, Room 2411A, Charlestown, MA 02129, USA
Stroke 39:1869-74. 2008..Emerging data suggest that neuroglobin (Ngb) may protect against hypoxic/ischemic neuronal insults. However, the underlying mechanisms in vivo and implications for long-term outcomes are still not well understood...
- Loss of insulin signaling in vascular endothelial cells accelerates atherosclerosis in apolipoprotein E null miceChristian Rask-Madsen
Research Division, Joslin Diabetes Center, Boston, MA 02215, USA
Cell Metab 11:379-89. 2010..Therefore, improving insulin sensitivity in the endothelium of patients with insulin resistance or type 2 diabetes may prevent cardiovascular complications...
- Endothelial nitric oxide synthase and endothelial dysfunctionPaul L Huang
Cardiovascular Research Center and Cardiovascular Division, Massachusetts General Hospital, 149 Thirteenth Street, Charlestown, MA 02129, USA
Curr Hypertens Rep 5:473-80. 2003..A hypothesis is proposed that the final common pathway of diverse causes of endothelial dysfunction involves abnormalities in eNOS phosphorylation at Ser 1179 and other key phosphorylation sites...
- Effects of chronic treatment with L-arginine on atherosclerosis in apoE knockout and apoE/inducible NO synthase double-knockout miceJiqiu Chen
Cardiovascular Research Center and Cardiology Division, Massachusetts General Hospital and Harvard Medical School, Boston, USA
Arterioscler Thromb Vasc Biol 23:97-103. 2003..In the present study, we investigate the effect of L-arginine supplementation on the development of atherosclerosis in a mouse model...
- Inducible nitric-oxide synthase is an important contributor to prolonged protective effects of ischemic preconditioning in the mouse kidneyKwon Moo Park
Renal Division, Department of Medicine, Brigham and Women s Hospital, Boston, Massachusetts 02115, USA
J Biol Chem 278:27256-66. 2003....
- Tissue plasminogen activator promotes matrix metalloproteinase-9 upregulation after focal cerebral ischemiaKiyoshi Tsuji
Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA
Stroke 36:1954-9. 2005..Here, we used a combination of pharmacological and genetic approaches to show that tPA promotes MMP-9 levels in stroke in vivo...
- In vivo imaging of proteolytic activity in atherosclerosisJiqiu Chen
Cardiology Division, Massachusetts General Hospital, Harvard Medical School, Boston, USA
Circulation 105:2766-71. 2002..On the basis of the hypothesis that the inflammatory response and proteolysis lead to plaque rupture, we have examined the role of cathepsin B as a model proteolytic enzyme...
- Deletion of exon 6 of the neuronal nitric oxide synthase gene in mice results in hypogonadism and infertilityRobert Gyurko
Cardiovascular Research Center and Reproductive Endocrine Unit, Massachusetts General Hospital and Harvard Medical School, Boston 02129, USA
Endocrinology 143:2767-74. 2002..We conclude that nNOS is required for normal central hormonal regulation of reproductive function...
- Soluble guanylate cyclase alpha1beta1 limits stroke size and attenuates neurological injuryDmitriy N Atochin
Cardiovascular Research Center and Cardiology Division, Massachusetts General Hospital, Boston, Mass, USA
Stroke 41:1815-9. 2010..To study the role of sGC in stroke injury, we compared the outcome of cerebral ischemia and reperfusion in mice deficient in the alpha(1) subunit of sGC (sGCalpha(1)(-/-)) with that in wild-type mice...
- Nitric oxide regulates the heart by spatial confinement of nitric oxide synthase isoformsLili A Barouch
Department of Medicine Cardiology Division, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21287, USA
Nature 416:337-9. 2002..Thus, NOS1 and NOS3 mediate independent, and in some cases opposite, effects on cardiac structure and function...
- Stimulation of nitric oxide synthase in cerebral cortex due to elevated partial pressures of oxygen: an oxidative stress responseStephen R Thom
Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia, PA 19104, USA
J Neurobiol 51:85-100. 2002..We conclude that hyperoxia causes an increase in (*)NO synthesis as part of a response to oxidative stress. Mechanisms for nNOS activation include augmentation in the association with Hsp90 and intracellular entry of calcium...
- Decreased arteriolar density in endothelial nitric oxide synthase knockout mice is due to hypertension, not to the constitutive defect in endothelial nitric oxide synthase enzymeNathalie Kubis
INSERM Unit 541, Hopital Lariboisiere, Paris, France
J Hypertens 20:273-80. 2002..Hypertension in endothelial nitric oxide synthase knockout (eNOS-/-) mice is believed to be partly due to altered vasodilatation. However, nitric oxide (NO) is also known to play an important part in angiogenesis...
- Contributions of nitric oxide synthase isoforms to pulmonary oxygen toxicity, local vs. mediated effectsIvan T Demchenko
Center for Hyperbaric Medicine and Environmental Physiology, Duke University Medical Center, Durham, NC 27710, USA
Am J Physiol Lung Cell Mol Physiol 294:L984-90. 2008..In addition, we demonstrated that nitric oxide derived from nNOS is involved in a neurogenic mechanism of HBO2-induced lung injury that is linked to central nervous system oxygen toxicity through adrenergic/cholinergic pathways...
- Mice lacking inducible nitric oxide synthase demonstrate impaired killing of Porphyromonas gingivalisRobert Gyurko
Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston University, Massachusetts 02118, USA
Infect Immun 71:4917-24. 2003..gingivalis. These results indicate that modulation of superoxide levels is a mechanism by which NO influences PMN function and that NO is an important element of the host defense against P. gingivalis...
- Lack of nitric oxide synthase depresses ion transporting enzyme function in cardiac muscleLan Zhou
Department of Medicine, Division of Cardiology, The Johns Hopkins University School of Medicine, Baltimore, USA
Biochem Biophys Res Commun 294:1030-5. 2002....
- Long-term treatment with N(omega)-nitro-L-arginine methyl ester causes arteriosclerotic coronary lesions in endothelial nitric oxide synthase-deficient miceOsamu Suda
Second Department of Internal Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan
Circulation 106:1729-35. 2002..We addressed this point in mice that are deficient in the endothelial NO synthase gene (eNOS-KO mice)...
- Vasculoprotective roles of neuronal nitric oxide synthaseTsuyoshi Morishita
Second Department of Internal Medicine, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan
FASEB J 16:1994-6. 2002..These results provide the first direct evidence that nNOS plays important roles in suppressing arteriosclerotic vascular lesion formation in vivo...
- [Mice lacking the eNOS gene]Masao Moroi
Division of Cardiovascular Medicine, Toho University School of Medicine, Ohashi Hospital
Nihon Rinsho 62:469-73. 2004
- Oxygen seizure latency and peroxynitrite formation in mice lacking neuronal or endothelial nitric oxide synthasesIvan T Demchenko
Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St Petersburg, 194223, Russia
Neurosci Lett 344:53-6. 2003....
- Nitric oxide protects cardiac sarcolemmal membrane enzyme function and ion active transport against ischemia-induced inactivationKai Y Xu
Department of Medicine, Division of Cardiology, Johns Hopkins Medical Institutions, Baltimore, Maryland 21224, USA
J Biol Chem 278:41798-803. 2003..These novel findings indicate that NO. can protect cardiac SL NOSs and (Na+ + K+)-ATPase against ischemia-induced inactivation and suggest that locally generated NO. may serve to regulate SL Na+/K+ ion active transport in the heart...
- Noncholinergic penile erection in mice lacking the gene for endothelial nitric oxide synthaseArthur L Burnett
Department of Urology, The James Buchanan Brady Urological Institute, The Johns Hopkins Hospital and The Johns Hopkins University School of Medicine, Baltimore, Maryland 21287 2411, USA
J Androl 23:92-7. 2002..We concluded that eNOS-/- mice preserve erectile function on the basis of a noncholinergic but NO-dependent mechanism and that eNOS physiologically mediates penile erection under cholinergic stimulation...
- NITRIC OXIDE SYNTHASE ISOFORMS IN ATHEROSCLEROSISPaul Huang; Fiscal Year: 2006..We propose to use bone marrow transplantation studies to assess the importance of NOS isoform expression by bone marrow-derived cells to vessel injury and atherosclerosis in vivo. ..
- Nitric Oxide and Mechanisms of NeuroprotectionPaul Huang; Fiscal Year: 2006..3. Test the hypothesis that Akt activation mediates neuroprotection in ischemic preconditioning and in global ischemia. ..
- ATHEROSCLEROSIS AND CEREBRAL ISCHEMIAPaul Huang; Fiscal Year: 2007..They also provide proof of principle for approaches that directly target cerebral vascular dysfunction for the treatment and prevention of stroke. ..
- eNOS phosphorylation in cerebrovascular function and strokePaul L Huang; Fiscal Year: 2010..This may well lead to the development of new treatment options directly addressing the links between metabolic diseases and (cerebro)vascular disease. ..
- NITRIC OXIDE SYNTHASE ISOFORMS IN ATHEROSCLEROSISPaul Huang; Fiscal Year: 2003..We propose to use bone marrow transplantation studies to assess the importance of NOS isoform expression by bone marrow-derived cells to vessel injury and atherosclerosis in vivo. ..
- NOS ISOFORMS IN CEREBRAL BLOOD FLOWPaul Huang; Fiscal Year: 2001....
- NITRIC OXIDE SYNTHASE ISOFORMS IN ATHEROSCLEROSISPaul Huang; Fiscal Year: 2002....
- eNOS phosphorylation in cerebrovascular function and strokePaul Huang; Fiscal Year: 2009..This may well lead to the development of new treatment options directly addressing the links between metabolic diseases and (cerebro)vascular disease. ..