Jason B Harris

Summary

Affiliation: Massachusetts General Hospital
Country: USA

Publications

  1. pmc High depth, whole-genome sequencing of cholera isolates from Haiti and the Dominican Republic
    Rachel Sealfon
    Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA, USA
    BMC Genomics 13:468. 2012
  2. pmc Susceptibility to Vibrio cholerae infection in a cohort of household contacts of patients with cholera in Bangladesh
    Jason B Harris
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA
    PLoS Negl Trop Dis 2:e221. 2008
  3. pmc Clinical outcomes in household contacts of patients with cholera in Bangladesh
    Ana A Weil
    International Centre for Diarrhoeal Disease Research Dhaka, Bangladesh
    Clin Infect Dis 49:1473-9. 2009
  4. pmc Immunologic responses to Vibrio cholerae in patients co-infected with intestinal parasites in Bangladesh
    Jason B Harris
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA
    PLoS Negl Trop Dis 3:e403. 2009
  5. pmc Blood group, immunity, and risk of infection with Vibrio cholerae in an area of endemicity
    Jason B Harris
    Division of Infectious Diseases, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA
    Infect Immun 73:7422-7. 2005
  6. pmc Identification of in vivo-induced bacterial protein antigens during human infection with Salmonella enterica serovar Typhi
    Jason B Harris
    Division of Infectious Diseases, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA
    Infect Immun 74:5161-8. 2006
  7. pmc Immune responses to O-specific polysaccharide and lipopolysaccharide of Vibrio cholerae O1 Ogawa in adult Bangladeshi recipients of an oral killed cholera vaccine and comparison to responses in patients with cholera
    Taher Uddin
    Centre for Vaccine Sciences, International Centre for Diarrhoeal Disease Research, Bangladesh ICDDR, B, Dhaka, Bangladesh NIDDK, LBC, National Institutes of Health, Bethesda, Maryland Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts Departments of Medicine and Pediatrics, Harvard Medical School, Boston, Massachusetts Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts
    Am J Trop Med Hyg 90:873-81. 2014
  8. pmc Characterization of anti-Salmonella enterica serotype Typhi antibody responses in bacteremic Bangladeshi patients by an immunoaffinity proteomics-based technology
    Richelle C Charles
    Division of Infectious Diseases, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA
    Clin Vaccine Immunol 17:1188-95. 2010
  9. pmc Antibody-secreting cell responses after Vibrio cholerae O1 infection and oral cholera vaccination in adults in Bangladesh
    Atiqur Rahman
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA
    Clin Vaccine Immunol 20:1592-8. 2013
  10. pmc In vivo expression of Salmonella enterica serotype Typhi genes in the blood of patients with typhoid fever in Bangladesh
    Alaullah Sheikh
    International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh
    PLoS Negl Trop Dis 5:e1419. 2011

Collaborators

Detail Information

Publications42

  1. pmc High depth, whole-genome sequencing of cholera isolates from Haiti and the Dominican Republic
    Rachel Sealfon
    Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, MA, USA
    BMC Genomics 13:468. 2012
    ..cholerae isolates to a high depth of coverage (>2000x); four of the seven isolates were previously sequenced...
  2. pmc Susceptibility to Vibrio cholerae infection in a cohort of household contacts of patients with cholera in Bangladesh
    Jason B Harris
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA
    PLoS Negl Trop Dis 2:e221. 2008
    ..Despite recent progress in understanding the molecular basis of Vibrio cholerae pathogenesis, there is relatively little knowledge of the factors that determine the variability in human susceptibility to V. cholerae infection...
  3. pmc Clinical outcomes in household contacts of patients with cholera in Bangladesh
    Ana A Weil
    International Centre for Diarrhoeal Disease Research Dhaka, Bangladesh
    Clin Infect Dis 49:1473-9. 2009
    ..cholerae infection and associated clinical symptoms in household contacts of patients with cholera and to identify risk factors for development of severe dehydration in this cohort...
  4. pmc Immunologic responses to Vibrio cholerae in patients co-infected with intestinal parasites in Bangladesh
    Jason B Harris
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA
    PLoS Negl Trop Dis 3:e403. 2009
    ..However, the immunomodulatory effects of concomitant intestinal parasitic infection in cholera patients have not been systematically evaluated...
  5. pmc Blood group, immunity, and risk of infection with Vibrio cholerae in an area of endemicity
    Jason B Harris
    Division of Infectious Diseases, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA
    Infect Immun 73:7422-7. 2005
    ..Based on this epidemiologic evidence, we propose a hypothesis for understanding the association between blood group O and the risk of infection with V. cholerae O1 and O139 as well as the risk of developing severe symptoms once infected...
  6. pmc Identification of in vivo-induced bacterial protein antigens during human infection with Salmonella enterica serovar Typhi
    Jason B Harris
    Division of Infectious Diseases, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA
    Infect Immun 74:5161-8. 2006
    ..Serovar Typhi antigens identified by IVIAT warrant further evaluation for their contributions to pathogenesis, and they may have diagnostic, therapeutic, or preventive uses...
  7. pmc Immune responses to O-specific polysaccharide and lipopolysaccharide of Vibrio cholerae O1 Ogawa in adult Bangladeshi recipients of an oral killed cholera vaccine and comparison to responses in patients with cholera
    Taher Uddin
    Centre for Vaccine Sciences, International Centre for Diarrhoeal Disease Research, Bangladesh ICDDR, B, Dhaka, Bangladesh NIDDK, LBC, National Institutes of Health, Bethesda, Maryland Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts Departments of Medicine and Pediatrics, Harvard Medical School, Boston, Massachusetts Department of Biochemistry and Molecular Biology, University of Dhaka, Bangladesh Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts
    Am J Trop Med Hyg 90:873-81. 2014
    ..The markedly lower ASC and MBC responses to OSP and LPS observed among vaccinees might explain, in part, the lower protection of an OCV compared with natural infection...
  8. pmc Characterization of anti-Salmonella enterica serotype Typhi antibody responses in bacteremic Bangladeshi patients by an immunoaffinity proteomics-based technology
    Richelle C Charles
    Division of Infectious Diseases, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA
    Clin Vaccine Immunol 17:1188-95. 2010
    ..Typhi-infected patients and detected anti-HlyE responses at the time of clinical presentation in patients but not in controls. These findings could assist in the development of improved diagnostic assays...
  9. pmc Antibody-secreting cell responses after Vibrio cholerae O1 infection and oral cholera vaccination in adults in Bangladesh
    Atiqur Rahman
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA
    Clin Vaccine Immunol 20:1592-8. 2013
    ..Given these results, it is likely that the optimal boosting strategies for OCVs differ significantly between areas where V. cholerae infection is endemic and those where it is not. ..
  10. pmc In vivo expression of Salmonella enterica serotype Typhi genes in the blood of patients with typhoid fever in Bangladesh
    Alaullah Sheikh
    International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh
    PLoS Negl Trop Dis 5:e1419. 2011
    ..Salmonella enterica serotype Typhi is the cause of typhoid fever. It is a human-restricted pathogen, and few data exist on S. Typhi gene expression in humans...
  11. pmc Development of immunoglobulin M memory to both a T-cell-independent and a T-cell-dependent antigen following infection with Vibrio cholerae O1 in Bangladesh
    Emily A Kendall
    International Centre for Diarrheal Disease Research, Bangladesh, Dhaka, Bangladesh
    Infect Immun 78:253-9. 2010
    ..We did not observe an association between the IgM memory response to LPS and the vibriocidal titer...
  12. pmc Comparison of immune responses to the O-specific polysaccharide and lipopolysaccharide of Vibrio cholerae O1 in Bangladeshi adult patients with cholera
    Russell A Johnson
    Centre for Vaccine Sciences, International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh
    Clin Vaccine Immunol 19:1712-21. 2012
    ..Induction of anti-OSP responses may be associated with protection against cholera, and our results may support the development of a vaccine targeting V. cholerae OSP...
  13. pmc Memory B cell and other immune responses in children receiving two doses of an oral killed cholera vaccine compared to responses following natural cholera infection in Bangladesh
    Daniel T Leung
    Centre for Vaccine Sciences, Bangladesh, Dhaka, Bangladesh
    Clin Vaccine Immunol 19:690-8. 2012
    ..These findings may explain, in part, the lower efficacy of oral cholera vaccination in children...
  14. pmc Antigen-specific memory B-cell responses to Vibrio cholerae O1 infection in Bangladesh
    AARON M HARRIS
    International Centre for Diarrheal Disease Research, Bangladesh, Centre for Health and Population Research, Dhaka, Bangladesh
    Infect Immun 77:3850-6. 2009
    ..cholerae protein antigens. Such memory B cells could mediate anamnestic responses on reexposure to V. cholerae...
  15. pmc Identification of immunogenic Salmonella enterica serotype Typhi antigens expressed in chronic biliary carriers of S. Typhi in Kathmandu, Nepal
    Richelle C Charles
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA
    PLoS Negl Trop Dis 7:e2335. 2013
    ..Little is known about the interaction between the host and pathogen in the biliary tract of chronic carriers, and there is currently no reliable diagnostic assay to identify asymptomatic S. Typhi carriage...
  16. pmc Antigen-specific memory B-cell responses in Bangladeshi adults after one- or two-dose oral killed cholera vaccination and comparison with responses in patients with naturally acquired cholera
    Mohammad Murshid Alam
    Immunology Unit, Laboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Bangladesh, Mohakali, Dhaka 1212, Bangladesh
    Clin Vaccine Immunol 18:844-50. 2011
    ..These findings may explain in part the relatively short-term protection afforded by oral cholera vaccination compared to natural disease...
  17. pmc Complexity of rice-water stool from patients with Vibrio cholerae plays a role in the transmission of infectious diarrhea
    Eric J Nelson
    Howard Hughes Medical Institute and Department of Molecular Biology and Microbiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, MA 02111, USA
    Proc Natl Acad Sci U S A 104:19091-6. 2007
    ..cholerae. If lytic phage are present, viable counts of V. cholerae drop, stools become DF(-), other microorganisms bloom, and cholera transmission is reduced...
  18. pmc Identification of in vivo-induced bacterial proteins during human infection with Salmonella enterica serotype Paratyphi A
    Mohammad Murshid Alam
    International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh
    Clin Vaccine Immunol 20:712-9. 2013
    ..S. Paratyphi A antigens identified by IVIAT warrant further evaluation for their contributions to pathogenesis and might have diagnostic, therapeutic, or preventive relevance...
  19. pmc Vibrio cholerae O1 infection induces proinflammatory CD4+ T-cell responses in blood and intestinal mucosa of infected humans
    Alison Kuchta
    International Centre for Diarrheal Disease Research, Bangladesh, Dhaka, Bangladesh
    Clin Vaccine Immunol 18:1371-7. 2011
    ..Additional studies are needed to determine whether these early T-cell-mediated events predict the subsequent duration of immunologic memory...
  20. pmc Diarrheal epidemics in Dhaka, Bangladesh, during three consecutive floods: 1988, 1998, and 2004
    Brian S Schwartz
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, USA
    Am J Trop Med Hyg 74:1067-73. 2006
    ..Our findings suggest that cholera is the predominant cause of flood-associated diarrheal epidemics in Dhaka, but that other organisms spread by the fecal-oral route also contribute...
  21. pmc Transcutaneous immunization with toxin-coregulated pilin A induces protective immunity against Vibrio cholerae O1 El Tor challenge in mice
    Julianne E Rollenhagen
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Infect Immun 74:5834-9. 2006
    ..001). Our results suggest that transcutaneous immunization with TcpA and an immunoadjuvant induces protective anti-TcpA immune responses. Anti-TcpA responses may contribute to an optimal cholera vaccine...
  22. pmc Frequency of reexposure to Vibrio cholerae O1 evaluated by subsequent vibriocidal titer rise after an episode of severe cholera in a highly endemic area in Bangladesh
    Ana A Weil
    Department of Medicine, Harvard Medical School, Boston, MA, USA
    Am J Trop Med Hyg 87:921-6. 2012
    ..Repeated exposures to V. cholerae in endemic areas may be a necessary component for long-lasting protection against severe disease...
  23. pmc Proteomic analysis of Vibrio cholerae in human stool
    Regina C LaRocque
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts 02114, USA
    Infect Immun 76:4145-51. 2008
    ..We identified a number of these in vivo expressed proteins as immunogenic during human infection. To our knowledge, this is the first characterization of the proteome of a pathogenic bacteria recovered from a natural host...
  24. pmc Relatedness of Vibrio cholerae O1/O139 isolates from patients and their household contacts, determined by multilocus variable-number tandem-repeat analysis
    Emily A Kendall
    International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh
    J Bacteriol 192:4367-76. 2010
    ..Our results demonstrate that genetically related V. cholerae strains cluster in local outbreaks but also suggest that multiple distinct strains of V. cholerae O1 may circulate simultaneously within a household...
  25. ncbi request reprint Postgenomic approaches to cholera vaccine development
    Regina C LaRocque
    Division of Infectious Diseases, Massachusetts General Hospital, GRJ 504, 55 Fruit Street, Boston, MA 02114, USA
    Expert Rev Vaccines 5:337-46. 2006
    ..This work holds promise for the identification of bacterial targets of protective human immune responses and may contribute to the development of a new generation of cholera vaccines...
  26. pmc Mucosal immunologic responses in cholera patients in Bangladesh
    Taher Uddin
    International Centre for Diarrhoeal Disease Research, Bangladesh, Centre for Health and Population Research, Dhaka, Bangladesh
    Clin Vaccine Immunol 18:506-12. 2011
    ....
  27. pmc Cholera
    Jason B Harris
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA, USA
    Lancet 379:2466-76. 2012
    ..Prevention of cholera depends on access to safe water and sanitation. Two oral cholera vaccines are available and the most effective use of these in integrated prevention programmes is being actively assessed...
  28. pmc Transcriptional profiling of Vibrio cholerae recovered directly from patient specimens during early and late stages of human infection
    Regina C LaRocque
    Division of Infectious Diseases, Gray Jackson 504, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114, USA
    Infect Immun 73:4488-93. 2005
    ..These findings provide a more detailed assessment of the transcriptome of V. cholerae in the human host than previous studies of organisms in stool alone and have implications for cholera control and the design of improved vaccines...
  29. pmc Cholera transmission: the host, pathogen and bacteriophage dynamic
    Eric J Nelson
    Howard Hughes Medical Institute, Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA
    Nat Rev Microbiol 7:693-702. 2009
    ....
  30. pmc Comparative proteomic analysis of the PhoP regulon in Salmonella enterica serovar Typhi versus Typhimurium
    Richelle C Charles
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA
    PLoS ONE 4:e6994. 2009
    ..The PhoP regulon is a well studied two component (PhoP/Q) coordinately regulated network of genes whose expression is required for intracellular survival of S. enterica...
  31. pmc Analysis of Salmonella enterica serotype paratyphi A gene expression in the blood of bacteremic patients in Bangladesh
    Alaullah Sheikh
    International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, Bangladesh
    PLoS Negl Trop Dis 4:e908. 2010
    ..Salmonella enterica serotype Paratyphi A is a human-restricted cause of paratyphoid fever, accounting for up to a fifth of all cases of enteric fever in Asia...
  32. pmc LPLUNC1 modulates innate immune responses to Vibrio cholerae
    Ok S Shin
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114, USA
    J Infect Dis 204:1349-57. 2011
    ..However, no functional role for LPLUNC1 has been identified...
  33. pmc Cellular and Cytokine Responses to Salmonella enterica Serotype Typhi Proteins in Patients with Typhoid Fever in Bangladesh
    Saruar Bhuiyan
    Division of Vaccine Sciences, International Centre for Diarrhoeal Disease Research, Bangladesh, ICDDR, B, Dhaka, Bangladesh Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts Department of Medicine, Harvard Medical School, Boston, Massachusetts Department of Microbiology and Immunobiology, Harvard Medical School, Boston, Massachusetts Virginia G Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, Arizona Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts
    Am J Trop Med Hyg 90:1024-30. 2014
    ..These results suggest that S. Typhi antigens induce a predominantly Th1 response, but that elevations in other cytokines may be modulatory. ..
  34. pmc Immunoproteomic analysis of antibody in lymphocyte supernatant in patients with typhoid fever in Bangladesh
    Richelle C Charles
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, USA
    Clin Vaccine Immunol 21:280-5. 2014
    ..These observations suggest that purified antigens could be used with ALS and corresponding acute-phase activated B lymphocytes in diagnostic platforms to identify acutely infected patients, even in areas where enteric fever is endemic. ..
  35. pmc Comparison of memory B cell, antibody-secreting cell, and plasma antibody responses in young children, older children, and adults with infection caused by Vibrio cholerae O1 El Tor Ogawa in Bangladesh
    Daniel T Leung
    International Centre for Diarrhoeal Disease Research, Bangladesh ICDDR, B, Dhaka, Bangladesh
    Clin Vaccine Immunol 18:1317-25. 2011
    ..cholerae O1, suggesting that under an optimal vaccination strategy, young children could achieve protective efficacy comparable to that induced in adults...
  36. pmc Immunogenicity of a killed bivalent (o1 and o139) whole cell oral cholera vaccine, shanchol, in haiti
    Richelle C Charles
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, United States of America Department of Medicine, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Negl Trop Dis 8:e2828. 2014
    ..There is a need to assess the immunogenicity of the vaccine in Haiti and other populations without historical exposure to Vibrio cholerae...
  37. pmc Evaluation in mice of a conjugate vaccine for cholera made from Vibrio cholerae O1 (Ogawa) O-specific polysaccharide
    Mohammad Murshid Alam
    Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, United States of America International Centre for Diarrheal Disease Research, Bangladesh ICDDR, B, Dhaka, Bangladesh
    PLoS Negl Trop Dis 8:e2683. 2014
    ..Serogroup specificity in Vibrio cholerae is determined by the O-specific polysaccharide (OSP) of lipopolysaccharide (LPS). Generally, polysaccharides are poorly immunogenic, especially in young children...
  38. ncbi request reprint Cholera: lessons from haiti and beyond
    Ana A Weil
    Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Curr Infect Dis Rep 14:1-8. 2012
    ..This review covers important features of epidemiology, pathogenesis, treatment and prevention of cholera, with a focus on the ongoing epidemic in Haiti...
  39. pmc Hyperinfectivity of human-passaged Vibrio cholerae can be modeled by growth in the infant mouse
    Ashfaqul Alam
    International Centre for Diarrhoeal Disease Research, Dhaka, Bangladesh
    Infect Immun 73:6674-9. 2005
    ..cholerae as well as the contribution of three type IV pili to the phenotype...
  40. pmc Reduction in capsular content and enhanced bacterial susceptibility to serum killing of Vibrio cholerae O139 associated with the 2002 cholera epidemic in Bangladesh
    Firdausi Qadri
    International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka
    Infect Immun 73:6577-83. 2005
    ..cholerae O139. Consequently, this assay may be useful in studies of both O139-infected patients and recipients of O139 vaccines...
  41. ncbi request reprint Incomplete correlation of serum vibriocidal antibody titer with protection from Vibrio cholerae infection in urban Bangladesh
    Debasish Saha
    International Centre for Diarrheal Disease Research, Bangladesh
    J Infect Dis 189:2318-22. 2004
    ..No association between baseline vibriocidal titer and protection from V. cholerae O139 infection was found. Our findings suggest that the vibriocidal antibody is an incomplete predictor of protection from V. cholerae infection...
  42. pmc Cholera toxin-specific memory B cell responses are induced in patients with dehydrating diarrhea caused by Vibrio cholerae O1
    Channa R Jayasekera
    International Centre for Diarrhoeal Disease Research, Bangladesh
    J Infect Dis 198:1055-61. 2008
    ..The presence of antigen-specific memory B cells may therefore be a more direct measure of protection than serum antibody responses...

Research Grants2

  1. Development of a small molecule screen for PhoP regulon inhibitors in Salmonella
    Jason Harris; Fiscal Year: 2007
    ..In future studies, compounds identified using the proposed assays may be developed into a new class of drugs targeted for the treatment of many bacterial infections, including several category A and B priority pathogens. ..