Frank GertlerSummaryAffiliation: Massachusetts Institute of Technology Country: USA Publications
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Publications
Mena deficiency delays tumor progression and decreases metastasis in polyoma middle-T transgenic mouse mammary tumorsEvanthia T Roussos
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
Breast Cancer Res 12:R101. 2010..Whether Mena is required for tumor progression is still unknown. Here we report the effects of Mena deficiency on tumor progression, metastasis and on normal mammary gland development...- Simultaneous imaging of GFP, CFP and collagen in tumors in vivo using multiphoton microscopyErik Sahai
Tumour Cell Biology Laboratory, Cancer Research UK London Research Institute, London WC2A 3PX, UK
BMC Biotechnol 5:14. 2005..However, the use of genetically encoded fluorescent proteins to distinguish different cell types in living animals has not been described at single cell resolution using multiphoton microscopy... - Metastasis: tumor cells becoming MENAcingFrank Gertler
Department of Biology and Koch Institute for Integrative Cancer Research at Massachusetts Institute of Technology MIT, 77 Massachusetts Ave, Cambridge, MA 02138, USA
Trends Cell Biol 21:81-90. 2011..This change in Mena isoform expression enables tumor cells to invade in response to otherwise benign EGF stimulus levels and could offer an opportunity to identify metastatic risk in patients... 
Arp2/3 is a negative regulator of growth cone translocationGeraldine A Strasser
Biology Department, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Neuron 43:81-94. 2004..Surprisingly, Arp2/3 inhibition significantly enhances axon elongation and causes defects in growth cone guidance. These results indicate that Arp2/3 is a negative regulator of growth cone translocation...- Critical role of Ena/VASP proteins for filopodia formation in neurons and in function downstream of netrin-1Cecile Lebrand
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Neuron 42:37-49. 2004..We propose that Ena/VASP proteins control filopodial dynamics in neurons by remodeling the actin network in response to guidance cues... - Cytoskeletal dynamics and transport in growth cone motility and axon guidanceErik W Dent
Biology Department, 68 270, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Neuron 40:209-27. 2003..An important goal for the future will be to understand the coordinated response of the cytoskeleton to signaling cascades induced by guidance receptor activation... - Ena/VASP Is Required for neuritogenesis in the developing cortexAdam V Kwiatkowski
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Neuron 56:441-55. 2007..These findings provide insight into the regulation of neurite formation and the role of the actin cytoskeleton during cortical development... - Mena and vasodilator-stimulated phosphoprotein are required for multiple actin-dependent processes that shape the vertebrate nervous systemA Sheila Menzies
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA
J Neurosci 24:8029-38. 2004..Mena-/-VASP-/- double mutants die perinatally and display defects in neurulation, craniofacial structures, and the formation of several fiber tracts in the CNS and peripheral nervous system... - Filopodia: the fingers that do the walkingStephanie L Gupton
Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Sci STKE 2007:re5. 2007..We discuss established and novel proteins that mediate the formation and dynamics of filopodia, different mechanisms of filopodium formation, and the various functions that distinct filopodia perform... - Function and regulation of Ena/VASP proteinsAdam V Kwiatkowski
Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Trends Cell Biol 13:386-92. 2003..This review will examine the mechanisms by which Ena/VASP function might control actin dynamics and how these proteins are linked to various signaling pathways... - Ena/VASP proteins: regulators of the actin cytoskeleton and cell migrationMatthias Krause
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Annu Rev Cell Dev Biol 19:541-64. 2003..Phosphorylation by these kinases appears to modulate Ena/VASP function within cells, although the mechanism underlying this regulation remains to be determined... - The Ena/VASP enigmaMatthias Krause
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139-4307, USA
J Cell Sci 115:4721-6. 2002..Recent discoveries resolve these contradictions and suggest a novel mechanism of Ena/VASP function, in which the proteins function as 'anti-capping' proteins that antagonize capping proteins at the barbed end of actin filaments... - Ena/VASP: proteins at the tip of the nervous systemFrauke Drees
David H Koch Institute for Integrative Cancer Research at MIT, Massachusetts Institute of Technology, Cambridge, MA 02139 4307, USA
Curr Opin Neurobiol 18:53-9. 2008..Here we review recent findings into Ena/VASP function in neurite initiation, axon outgrowth and guidance... - Critical roles of phosphorylation and actin binding motifs, but not the central proline-rich region, for Ena/vasodilator-stimulated phosphoprotein (VASP) function during cell migrationJoseph J Loureiro
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA
Mol Biol Cell 13:2533-46. 2002..Interestingly, expression of the C-terminal EVH2 domain alone is sufficient to complement loss of Ena/VASP function in random cell motility... - Shigella interactions with the actin cytoskeleton in the absence of Ena/VASP family proteinsShabeen Ally
Infectious Disease Division, Massachusetts General Hospital, Cambridge, MA 02139, USA
Cell Microbiol 6:355-66. 2004..Thus, although VASP is recruited to the surface of intracellular Shigella, it is not essential for Shigella actin-based motility... - Ena/VASP proteins regulate cortical neuronal positioningKeow Lin Goh
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Curr Biol 12:565-9. 2002..The abnormally placed pyramidal neurons exhibited grossly normal morphology and polarity. Our results are consistent with a model in which Ena/VASP proteins function in vivo to control the position of neurons in the mouse neocortex... - Integrin signaling switches the cytoskeletal and exocytic machinery that drives neuritogenesisStephanie L Gupton
The Koch Institute for Integrative Cancer Research at MIT, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Dev Cell 18:725-36. 2010..These regulated changes and coordination of cytoskeletal and exocytic machinery may be used in other physiological contexts involving cell motility and morphogenesis... - Ena/VASP is required for endothelial barrier function in vivoCraig Furman
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
J Cell Biol 179:761-75. 2007..These findings demonstrate that Ena/VASP is critical for actin cytoskeleton remodeling events involved in the maintenance of functional endothelia... - Lamellipodin, an Ena/VASP ligand, is implicated in the regulation of lamellipodial dynamicsMatthias Krause
Department of Biology and Center for Cancer Research, MIT, Cambridge, MA 02139, USA
Dev Cell 7:571-83. 2004..These phenotypes are more severe than loss of Ena/VASP, suggesting that Lpd regulates other effectors of the actin cytoskeleton in addition to Ena/VASP... - Filopodia are required for cortical neurite initiationErik W Dent
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Nat Cell Biol 9:1347-59. 2007..Neurite initiation also requires microtubule extension into filopodia, suggesting that interactions between actin-filament bundles and dynamic microtubules within filopodia are crucial for neuritogenesis... - In vivo RNAi screening identifies regulators of actin dynamics as key determinants of lymphoma progressionCorbin E Meacham
The Koch Institute for Integrative Cancer Research at MIT, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
Nat Genet 41:1133-7. 2009.... - A Mena invasion isoform potentiates EGF-induced carcinoma cell invasion and metastasisUlrike Philippar
Massachusetts Institute of Technology, Koch Institute, Cambridge, MA 02139, USA
Dev Cell 15:813-28. 2008..Upregulation of Mena(INV) could therefore enable tumor cells to invade in response to otherwise benign EGF stimulus levels... - Epidermal growth factor-induced enhancement of glioblastoma cell migration in 3D arises from an intrinsic increase in speed but an extrinsic matrix- and proteolysis-dependent increase in persistenceHyung Do Kim
Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Mol Biol Cell 19:4249-59. 2008..Thus, the EGF-enhanced 3D tumor cell migration results only partially from cell-intrinsic effects, with override of cell-intrinsic persistence decrease by protease-mediated cell-extrinsic reduction of matrix steric hindrance... - Nervous Rac: DOCK7 regulation of axon formationElaine Maria Pinheiro
Department of Biology, Massachusetts Institute of Technology, Cambridge, 02139, USA
Neuron 51:674-6. 2006..Microtubules play an important role in neuronal polarity. In this issue of Neuron, Watabe-Uchida et al. link a novel Rac-mediated pathway that regulates microtubule dynamics to axon formation... - Tuba stimulates intracellular N-WASP-dependent actin assemblyEva M Kovacs
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
J Cell Sci 119:2715-26. 2006..These results suggest first that Tuba-stimulated dorsal ruffling might represent a novel mechanism for the coordination of N-WASP-dependent cytoskeletal rearrangements and second that Tuba function is implicated in motility processes... - A bivalent Huntingtin binding peptide suppresses polyglutamine aggregation and pathogenesis in DrosophilaAleksey Kazantsev
Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Nat Genet 30:367-76. 2002..These results suggest that targeting the protein interactions leading to aggregate formation may be beneficial for the design and development of therapeutic agents for Huntington disease... - Palladin is a novel binding partner for Ena/VASP family membersMalika Boukhelifa
Department of Cell and Molecular Physiology, University of North Carolina at Chapel Hill, 27599 7545, USA
Cell Motil Cytoskeleton 58:17-29. 2004..These results suggest that the recently described actin-associated protein palladin may play an important role in recruiting VASP to sites of actin filament growth, anchorage, and crosslinking... - Lamellipodial versus filopodial mode of the actin nanomachinery: pivotal role of the filament barbed endMarisan R Mejillano
Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, 303 E Chicago Avenue, Chicago, IL 60611, USA
Cell 118:363-73. 2004.... - Emergence of large-scale cell morphology and movement from local actin filament growth dynamicsCatherine I Lacayo
Department of Biochemistry, Stanford University, Stanford, California, United States of America
PLoS Biol 5:e233. 2007.... - Ena/VASP proteins contribute to Listeria monocytogenes pathogenesis by controlling temporal and spatial persistence of bacterial actin-based motilityVictoria Auerbuch
Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
Mol Microbiol 49:1361-75. 2003..Lastly, we showed the pathogenic relevance of these findings by the observation that L. monocytogenes lacking ActA Ena/VASP-binding sites were up to 400-fold less virulent during an adaptive immune response... - Ena/VASP function in retinal axons is required for terminal arborization but not pathway navigationAsha Dwivedy
Department of Anatomy, University of Cambridge, Downing Street, Cambridge CB2 3DY, UK
Development 134:2137-46. 2007.... - Ena/VASP proteins have an anti-capping independent function in filopodia formationDerek A Applewhite
Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
Mol Biol Cell 18:2579-91. 2007..Furthermore, the EVH1 domain, together with the GAB motif in the EVH2 domain, helps to maintain Ena/VASP at the growing barbed ends... - Contribution of Ena/VASP proteins to intracellular motility of listeria requires phosphorylation and proline-rich core but not F-actin binding or multimerizationMarcus Geese
Department of Cell Biology, , D-38124 Braunschweig, Germany
Mol Biol Cell 13:2383-96. 2002..Our data suggest that Ena/VASP proteins contribute to Listeria motility by regulating both the nucleation and elongation of actin filaments at the bacterial surface... - Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motilityJames E Bear
Massachusetts Institute of Technology, Department of Biology, Cambridge 02139, USA
Cell 109:509-21. 2002..We conclude that Ena/VASP regulates cell motility by controlling the geometry of actin filament networks within lamellipodia... - Targeted disruption of the murine zyxin geneLaura M Hoffman
Huntsman Cancer Institute and Department of Biology, University of Utah, Salt Lake City 84112, USA
Mol Cell Biol 23:70-9. 2003.... - The murine Nck SH2/SH3 adaptors are important for the development of mesoderm-derived embryonic structures and for regulating the cellular actin networkFriedhelm Bladt
Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada
Mol Cell Biol 23:4586-97. 2003..These data suggest that the Nck SH2/SH3 adaptors have important functions in the development of mesodermal structures during embryogenesis, potentially linked to a role in cell movement and cytoskeletal organization... - MIG-10/lamellipodin and AGE-1/PI3K promote axon guidance and outgrowth in response to slit and netrinChieh Chang
Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, New York 10021, USA
Curr Biol 16:854-62. 2006..In cultured cells, mammalian MIG-10 promotes lamellipodial growth and Ena/VASP proteins induce filopodia... - Tuba, a novel protein containing bin/amphiphysin/Rvs and Dbl homology domains, links dynamin to regulation of the actin cytoskeletonMarco A Salazar
Department of Cell Biology and the Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, Connecticut 06519, USA
J Biol Chem 278:49031-43. 2003..The properties of Tuba provide new evidence for a close functional link between dynamin, Rho GTPase signaling, and the actin cytoskeleton... - RIAM, an Ena/VASP and Profilin ligand, interacts with Rap1-GTP and mediates Rap1-induced adhesionEsther M Lafuente
Department of Medical Oncology, Dana Farber Cancer Institute, Department of Medicine, Brigham and Women s Hospital, Harvard Medical School, Boston, MA 02115, USA
Dev Cell 7:585-95. 2004..RIAM knockdown displaced Rap1-GTP from the plasma membrane and abrogated Rap1-induced adhesion. Thus, RIAM links Rap1 to integrin activation and plays a role in regulating actin dynamics... - WASP-related proteins, Abi1 and Ena/VASP are required for Listeria invasion induced by the Met receptorHelene Bierne
Unité des Interactions Bactéries Cellules, Institut Pasteur, INSERM U604, INRA USC2020, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France
J Cell Sci 118:1537-47. 2005.... - Enabled plays key roles in embryonic epithelial morphogenesis in DrosophilaJulie Gates
Lineberger Comprehensive Cancer Center and Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Development 134:2027-39. 2007..We also explored filopodial regulation in cultured Drosophila cells and embryos. These data provide new insights into developmental and mechanistic roles of this important actin regulator... - Ena/VASP proteins mediate repulsion from ephrin ligandsIwan R Evans
Department of Biochemistry, School of Medical Sciences, University of Bristol, University Walk, Bristol, BS8 1TD, UK
J Cell Sci 120:289-98. 2007..Our results suggest that Ena/VASP proteins regulate ephrin-induced Eph receptor signalling events, possibly by destabilising lamellipodial protrusions... - Ena/VASP proteins can regulate distinct modes of actin organization at cadherin-adhesive contactsJeanie A Scott
Division of Molecular Cell Biology, Institute for Molecular Bioscience, The University of Queensland, St. Lucia, Brisbane, Queensland, Australia 4072
Mol Biol Cell 17:1085-95. 2006..We propose that Ena/VASP proteins may serve as higher order regulators of the cytoskeleton at cadherin contacts through their ability to modulate distinct modes of actin organization at those contacts... - Human Mena+11a isoform serves as a marker of epithelial phenotype and sensitivity to epidermal growth factor receptor inhibition in human pancreatic cancer cell linesMaria S Pino
Division of Medical Oncology, Regina Elena National Cancer Institute, Rome, Italy
Clin Cancer Res 14:4943-50. 2008..The aim of this study was to determine whether or not the expression of hMena isoforms correlated with sensitivity to EGFR tyrosine kinase inhibitors and could serve as markers with potential clinical use...
Research Grants
- Cellular and Developmental Function of MenaFrank B Gertler; Fiscal Year: 2011....
- Cellular and Developmental Function of MenaFrank B Gertler; Fiscal Year: 2010....
- Cytoskeletal Regulation During Growth Cone Migration and Axon GuidanceFrank B Gertler; Fiscal Year: 2010..We seek a comprehensive understanding of how nerve fibers form and are guided to their targets initially during fetal development expecting that this will help us learn to repair damage to the brain. ..
- Cellular and Developmental Function of MenaFrank Gertler; Fiscal Year: 2009....
- Cytoskeletal Regulation During Growth Cone Migration and Axon GuidanceFrank Gertler; Fiscal Year: 2009..We seek a comprehensive understanding of how nerve fibers form and are guided to their targets initially during fetal development expecting that this will help us learn to repair damage to the brain. ..
- CYTOSKELETAL REGULATION DURING GROWTH CONE GUIDANCEFrank Gertler; Fiscal Year: 2007..This work should yield valuable insight into how the nervous system develops and how to design methods to repair it after injury. ..
- CELLULAR AND DEVELOPMENTAL FUNCTIONS IN MENAFrank Gertler; Fiscal Year: 2007..Interestingly, Abi proteins are present in complexes that regulate the SCAR/WAVE family of Arp2/3 activating proteins, suggesting that NESH could serve as a link between Ena/VASP and SCAR/WAVE function. ..
- CYTOSKELETAL REGULATION DURING GROWTH CONE GUIDANCEFrank Gertler; Fiscal Year: 2006..This work should yield valuable insight into how the nervous system develops and how to design methods to repair it after injury. ..
- CELLULAR AND DEVELOPMENTAL FUNCTIONS OF MENAFrank Gertler; Fiscal Year: 2003..These Mena-deficient cells will also serve as an experimental system in which to conduct a structure-function analysis of the Mena protein. ..
- Cytoskeletal Regulation During Growth Cone Migration and Axon GuidanceFrank B Gertler; Fiscal Year: 2011..We seek a comprehensive understanding of how nerve fibers form and are guided to their targets initially during fetal development expecting that this will help us learn to repair damage to the brain. ..