Affiliation: Massachusetts Institute of Technology
- Fluorescent probes for super-resolution imaging in living cellsMarta Fernandez-Suarez
Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
Nat Rev Mol Cell Biol 9:929-43. 2008..We describe the features of existing super-resolution fluorophores and highlight areas of importance for future research and development...
- A fluorophore ligase for site-specific protein labeling inside living cellsChayasith Uttamapinant
Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 107:10914-9. 2010..By genetically targeting the PRIME ligase to specific subcellular compartments, we were able to selectively label spatially distinct subsets of proteins, such as the surface pool of neurexin and the nuclear pool of actin...
- Cells in fluidic environments are sensitive to flow frequencyMercedes Balcells
Harvard MIT Division of Health Sciences and Technology, Cambridge, Massachusetts, USA
J Cell Physiol 204:329-35. 2005..These findings suggest that frequency, independent of shear, is an essential determinant of cell response in pulsatile environments...
- Redirecting lipoic acid ligase for cell surface protein labeling with small-molecule probesMarta Fernandez-Suarez
Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, Massachusetts 02139, USA
Nat Biotechnol 25:1483-7. 2007..Our methodology should provide general access to biochemical and imaging studies of cell surface proteins, using small fluorophores introduced via a short peptide tag...