CATHERINE DRENNAN

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. ncbi request reprint The metalloclusters of carbon monoxide dehydrogenase/acetyl-CoA synthase: a story in pictures
    Catherine L Drennan
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Biol Inorg Chem 9:511-5. 2004
  2. pmc Life on carbon monoxide: X-ray structure of Rhodospirillum rubrum Ni-Fe-S carbon monoxide dehydrogenase
    C L Drennan
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 98:11973-8. 2001
  3. ncbi request reprint Surprising cofactors in metalloenzymes
    Catherine L Drennan
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA
    Curr Opin Struct Biol 13:220-6. 2003
  4. ncbi request reprint A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase
    Tzanko I Doukov
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 298:567-72. 2002
  5. pmc Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme
    Frederick Berkovitch
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 303:76-9. 2004
  6. ncbi request reprint Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis
    Leah C Blasiak
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 440:368-71. 2006
  7. ncbi request reprint Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme
    Luke J Higgins
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 437:838-44. 2005
  8. ncbi request reprint The crystal structure of class II ribonucleotide reductase reveals how an allosterically regulated monomer mimics a dimer
    Michael D Sintchak
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Struct Biol 9:293-300. 2002
  9. pmc Structural analysis of an open active site conformation of nonheme iron halogenase CytC3
    Cintyu Wong
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 131:4872-9. 2009
  10. ncbi request reprint Bringing the excitement of biological research into the chemistry classroom at MIT
    Elizabeth Vogel Taylor
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    ACS Chem Biol 2:515-7. 2007

Research Grants

  1. Complex Metallocluster Structure and Assembly
    CATHERINE DRENNAN; Fiscal Year: 2009
  2. X-ray studies of metalloenzyme regulation and catalysis
    CATHERINE DRENNAN; Fiscal Year: 2007
  3. Complex Metallocluster Structure and Assembly
    CATHERINE DRENNAN; Fiscal Year: 2007
  4. SUMMER RESEARCH IN MACROMOLECULAR INTERACTIONS
    CATHERINE DRENNAN; Fiscal Year: 2004
  5. Complex Metallocluster Structure and Assembly
    Catherine L Drennan; Fiscal Year: 2010

Collaborators

Detail Information

Publications36

  1. ncbi request reprint The metalloclusters of carbon monoxide dehydrogenase/acetyl-CoA synthase: a story in pictures
    Catherine L Drennan
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Biol Inorg Chem 9:511-5. 2004
    ....
  2. pmc Life on carbon monoxide: X-ray structure of Rhodospirillum rubrum Ni-Fe-S carbon monoxide dehydrogenase
    C L Drennan
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 98:11973-8. 2001
    ..The two Rossmann domains contribute ligands to the active site C-cluster. This x-ray structure provides insight into the mechanism of biological CO oxidation and has broader significance for the roles of Ni and Fe in biological systems...
  3. ncbi request reprint Surprising cofactors in metalloenzymes
    Catherine L Drennan
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139, USA
    Curr Opin Struct Biol 13:220-6. 2003
    ....
  4. ncbi request reprint A Ni-Fe-Cu center in a bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase
    Tzanko I Doukov
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 298:567-72. 2002
    ....
  5. pmc Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme
    Frederick Berkovitch
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Science 303:76-9. 2004
    ..The structure places the substrates between the Fe4S4 cluster, essential for radical generation, and the Fe2S2 cluster, postulated to be the source of sulfur, with both clusters in unprecedented coordination environments...
  6. ncbi request reprint Crystal structure of the non-haem iron halogenase SyrB2 in syringomycin biosynthesis
    Leah C Blasiak
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 440:368-71. 2006
    ..6 A resolution. This structure reveals a previously unknown coordination of iron, in which the carboxylate ligand of the facial triad is replaced by a chloride ion...
  7. ncbi request reprint Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme
    Luke J Higgins
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nature 437:838-44. 2005
    ....
  8. ncbi request reprint The crystal structure of class II ribonucleotide reductase reveals how an allosterically regulated monomer mimics a dimer
    Michael D Sintchak
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Struct Biol 9:293-300. 2002
    ..Thus, L. leichmannii RNR is a paradigm for the simplest structural entity capable of ribonucleotide reduction, a reaction linking the RNA and DNA worlds...
  9. pmc Structural analysis of an open active site conformation of nonheme iron halogenase CytC3
    Cintyu Wong
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Ave, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 131:4872-9. 2009
    ....
  10. ncbi request reprint Bringing the excitement of biological research into the chemistry classroom at MIT
    Elizabeth Vogel Taylor
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    ACS Chem Biol 2:515-7. 2007
  11. pmc Crystallographic trapping in the rebeccamycin biosynthetic enzyme RebC
    Katherine S Ryan
    Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 104:15311-6. 2007
    ....
  12. doi request reprint The violacein biosynthetic enzyme VioE shares a fold with lipoprotein transporter proteins
    Katherine S Ryan
    Department of Biology and Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Biol Chem 283:6467-75. 2008
    ..This structural and mutagenesis study suggests that VioE acts as a catalytic chaperone, using a fold previously associated with lipoprotein transporters to catalyze the production of its prodeoxyviolacein product...
  13. pmc Crystallographic snapshots of cyanide- and water-bound C-clusters from bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase
    Yan Kung
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 48:7432-40. 2009
    ..The crystal structures presented here, along with recent structures of CODHs from other organisms, have led us toward a unified mechanism for CO oxidation by the C-cluster, the catalytic center of an environmentally important enzyme...
  14. pmc First-principles study of non-heme Fe(II) halogenase SyrB2 reactivity
    Heather J Kulik
    Department of Materials Science and Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 131:14426-33. 2009
    ....
  15. ncbi request reprint Ribbon-helix-helix transcription factors: variations on a theme
    Eric R Schreiter
    Department of Chemistry and Protein Research Center, University of Puerto Rico at Rio Piedras, San Juan, Puerto Rico 00931, Puerto Rico
    Nat Rev Microbiol 5:710-20. 2007
    ....
  16. doi request reprint A research-inspired laboratory sequence investigating acquired drug resistance
    Elizabeth Vogel Taylor
    Departments of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
    Biochem Mol Biol Educ 38:247-52. 2010
    ..The exercises provide students with a hands-on understanding of the impact of biochemistry on human health, and demonstrate their potential as the next generation of investigators...
  17. doi request reprint The FAD cofactor of RebC shifts to an IN conformation upon flavin reduction
    Katherine S Ryan
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 47:13506-13. 2008
    ..Along with kinetic data presented here, these structures suggest that the first cycle of catalysis in RebC may resemble that of p-hydroxybenzoate hydroxylase, with substrate binding promoting flavin reduction...
  18. pmc Structural basis of low-affinity nickel binding to the nickel-responsive transcription factor NikR from Escherichia coli
    Christine M Phillips
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 49:7830-8. 2010
    ....
  19. pmc NikR-operator complex structure and the mechanism of repressor activation by metal ions
    Eric R Schreiter
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 103:13676-81. 2006
    ....
  20. doi request reprint Structural perspective on enzymatic halogenation
    Leah C Blasiak
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Acc Chem Res 42:147-55. 2009
    ....
  21. pmc Xenon in and at the end of the tunnel of bifunctional carbon monoxide dehydrogenase/acetyl-CoA synthase
    Tzanko I Doukov
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 47:3474-83. 2008
    ....
  22. pmc Structural basis for glycyl radical formation by pyruvate formate-lyase activating enzyme
    Jessica L Vey
    Departments of Chemistry and Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 105:16137-41. 2008
    ....
  23. pmc Physical basis of metal-binding specificity in Escherichia coli NikR
    Christine M Phillips
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Am Chem Soc 131:10220-8. 2009
    ..Taken together, these data suggest that the EcNikR structure is most stable when Ni(2+) occupies high-affinity sites and that EcNikR binding to DNA is more favorable when the second site contains K(+)...
  24. pmc Thioredoxin reductase from Thermoplasma acidophilum: a new twist on redox regulation
    Hector H Hernandez
    Department of Chemistry, Massachusetts Institute of Technology, 16 573, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Biochemistry 47:9728-37. 2008
    ..Our discovery of a TrxR that does not use NADPH provides a new twist in redox regulation...
  25. doi request reprint Structural basis of the metal specificity for nickel regulatory protein NikR
    Christine M Phillips
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 47:1938-46. 2008
    ..These structural findings along with biochemical data on NikR support a hypothesis that ordering of the alpha3 helix is important for repressor activation...
  26. pmc A locking mechanism preventing radical damage in the absence of substrate, as revealed by the x-ray structure of lysine 5,6-aminomutase
    Frederick Berkovitch
    Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 101:15870-5. 2004
    ..Thus, the structure embodies a locking mechanism to keep the adenosylcobalamin out of the active site and prevent radical generation in the absence of substrate...
  27. ncbi request reprint Crystal structure and mutagenesis of the metallochaperone MeaB: insight into the causes of methylmalonic aciduria
    Paul A Hubbard
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 282:31308-16. 2007
    ....
  28. pmc Searching for the Nik operon: how a ligand-responsive transcription factor hunts for its DNA binding site
    Christine M Phillips
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biochemistry 49:7757-63. 2010
    ..coli NikR and the cellular model for transcription factors in general, are in agreement, and the details laid out by the NikR system may lend additional credence to the current models for transcription factors searching for DNA...
  29. ncbi request reprint Crystal structure of the nickel-responsive transcription factor NikR
    Eric R Schreiter
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139 USA
    Nat Struct Biol 10:794-9. 2003
    ..The regulatory domain of NikR contains four nickel-binding sites at the tetramer interface, each exhibiting a novel square-planar coordination by three histidines and one cysteine side chain...
  30. pmc AdoMet radical proteins--from structure to evolution--alignment of divergent protein sequences reveals strong secondary structure element conservation
    Yvain Nicolet
    Department of Chemistry 16 573, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Nucleic Acids Res 32:4015-25. 2004
    ..These differences in barrel architecture also explain how AdoMet radical enzymes can act on substrates that range in size from 10 atoms to 608 residue proteins...
  31. doi request reprint Direct electrochemical analyses of a thermophilic thioredoxin reductase: interplay between conformational change and redox chemistry
    Michael J Hamill
    Department of Chemistry, Boston University, 590 Commonwealth Avenue, Boston, Massachusetts 02215, USA
    Biochemistry 47:9738-46. 2008
    ....
  32. ncbi request reprint Direct electrochemical characterization of archaeal thioredoxins
    Sarah E Chobot
    Department of Chemistry, Boston University, 590 Commonwealth Avenue, Boston, MA 02215, USA
    Angew Chem Int Ed Engl 46:4145-7. 2007
  33. ncbi request reprint Kinetic and structural characterization of Slr0077/SufS, the essential cysteine desulfurase from Synechocystis sp. PCC 6803
    Bhramara Tirupati
    Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
    Biochemistry 43:12210-9. 2004
    ....
  34. pmc Structures of the iron-sulfur flavoproteins from Methanosarcina thermophila and Archaeoglobus fulgidus
    Susana L A Andrade
    Institut fur Mikrobiologie und Genetik, Abt Molekulare Strukturbiologie, Georg August Universitat Gottingen, Justus von Liebig Weg 11, 37077 Gottingen, Germany
    J Bacteriol 187:3848-54. 2005
    ..This is only possible between different monomers upon oligomerization. Fundamental differences in the surface properties of the two ISF homologs underscore the diversity encountered within this protein family...
  35. ncbi request reprint Structural and kinetic evidence for an extended hydrogen-bonding network in catalysis of methyl group transfer. Role of an active site asparagine residue in activation of methyl transfer by methyltransferases
    Tzanko I Doukov
    Department of Biochemistry, Beadle Center, University of Nebraska, Lincoln, Nebraska 68588 0664, USA
    J Biol Chem 282:6609-18. 2007
    ....
  36. ncbi request reprint Chlorination by a long-lived intermediate in the mechanism of flavin-dependent halogenases
    Ellen Yeh
    Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA
    Biochemistry 46:1284-92. 2007
    ..This covalent enzyme chloramine likely plays a key role in directing regiospecific chlorination of substrate in this important class of biosynthetic enzymes...

Research Grants15

  1. Complex Metallocluster Structure and Assembly
    CATHERINE DRENNAN; Fiscal Year: 2009
    ..Our goals are to explore the mechanism and assembly of complex metallocofactors, as well as the cellular regulation of nickel uptake and iron-sulfur cluster biogenesis. ..
  2. X-ray studies of metalloenzyme regulation and catalysis
    CATHERINE DRENNAN; Fiscal Year: 2007
    ..We will complement structural data with biochemical characterization and single crystal spectroscopic techniques. ..
  3. Complex Metallocluster Structure and Assembly
    CATHERINE DRENNAN; Fiscal Year: 2007
    ..To explore cellular Ni uptake, we will investigate the structure and mechanism of Ni-regulatory protein NikR. ..
  4. SUMMER RESEARCH IN MACROMOLECULAR INTERACTIONS
    CATHERINE DRENNAN; Fiscal Year: 2004
    ..The proposed training project complements institutional goals of expanding career choices for students majoring in physical and quantitative sciences, and increasing minority participation in undergraduate research programs. ..
  5. Complex Metallocluster Structure and Assembly
    Catherine L Drennan; Fiscal Year: 2010
    ..Our goals are to explore the mechanism and assembly of complex metallocofactors, as well as the cellular regulation of nickel uptake and iron-sulfur cluster biogenesis. ..