Research Topics
Genomes and Genes | A L DemainSummaryAffiliation: Massachusetts Institute of Technology Country: USA Publications
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Detail Information
Publications
Secondary metabolism in simulated microgravityA L Demain
Biology Department, Massachusetts Institute of Technology, Cambridge, USA
Chem Rec 1:333-46. 2001..With respect to S. hygroscopicus, addition of Teflon beads to the RWB reversed the inhibition of growth, but rapamycin production was still markedly inhibited, and the distribution did not revert back to a preferential cellular site...- The natural functions of secondary metabolitesA L Demain
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
Adv Biochem Eng Biotechnol 69:1-39. 2000.... - Immobilized Streptomyces clavuligerus NP1 cells for biotransformation of penicillin G into deacetoxycephalosporin GA L Demain
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
Appl Biochem Biotechnol 87:135-40. 2000.... - Induction of microbial secondary metabolismA L Demain
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
Int Microbiol 1:259-64. 1998..The B-factor of A. mediterranei is an inducer of ansamycin (rifamycin) formation... - Microbial biotechnologyA L Demain
Biology Department, Massachusetts Institute of Technology, Cambridge 02139, USA
Trends Biotechnol 18:26-31. 2000..Today, microbiology is a major participant in global industry, especially in the pharmaceutical, food and chemical industries... - Pharmaceutically active secondary metabolites of microorganismsA L Demain
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
Appl Microbiol Biotechnol 52:455-63. 1999.... - The beta-lactam antibiotics: past, present, and futureA L Demain
Department of Biology, Massachusetts Intititute of Technology, Cambridge 02139, USA
Antonie Van Leeuwenhoek 75:5-19. 1999..Although seventy years of age, the beta-lactams are not yet ready for retirement... - Shear stress enhances microcin B17 production in a rotating wall bioreactor, but ethanol stress does notQ Gao
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
Appl Microbiol Biotechnol 56:384-7. 2001..Interestingly, cells growing in the bioreactor were much more resistant to the growth-inhibitory and production-inhibitory effects of ethanol than cells growing in shaken flasks... - Improvement in the bioconversion of penicillin G to deacetoxycephalosporin G by elimination of agitation and addition of decaneQ Gao
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
Appl Microbiol Biotechnol 57:511-3. 2001..Many additives to the medium were examined for their effect on the reaction. Clearly, the most effective compound was the organic solvent, decane... - Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin GQ Gao
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
J Ind Microbiol Biotechnol 30:190-4. 2003..Whereas NP1 preferred 1.8 mM FeSO(4 )and 1.3 mM alpha-ketoglutarate, recombinant W25 performed best at 0.45 mM FeSO(4) and 1.9 mM alpha-ketoglutarate... - Tetanus toxin production in soy-based medium: nutritional studies and scale-up into small fermentorsA L Demain
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA
Lett Appl Microbiol 45:635-8. 2007..To further improve the soy-based medium, devoid of animal and dairy products, for a production of tetanus toxin by nutritional studies and to scale-up the Clostridium tetani process into small fermentors... - Effect of solvents on bioconversion of penicillin G to deacetoxycephalosporin GQ Gao
Biology Department, Massachusetts Institute of Technology, Cambridge 02139, USA
J Antibiot (Tokyo) 54:958-61. 2001..Optimum conditions of pH and temperature for the alkane system are not very different from the previously used aqueous system... - Induction of microcin B17 formation in Escherichia coli ZK650 by limitation of oxygen and glucose is independent of glucose consumption rateQ Gao
Fermentation Microbiology Laboratory, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
J Ind Microbiol Biotechnol 26:341-4. 2001..0 mg/ml) under both high and low aeration. Glucose consumption rates were virtually identical under both high and low aeration. Thus, glucose consumption rate is not a regulating factor in microcin B17 formation... - Molecular genetics and industrial microbiology--30 years of marriageA L Demain
Biology Department, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
J Ind Microbiol Biotechnol 27:352-6. 2001..Agricultural biotechnology has made great strides but unfortunately its progress is being delayed by political controversy... - Menstrum for culture preservation and medium for seed preparation in a tetanus toxin production process containing no animal or dairy productsA Fang
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA
Lett Appl Microbiol 43:360-3. 2006..The new vegetable-based process described here avoids such unfortunate possibilities... - Improvement in the resting-cell bioconversion of penicillin G to deacetoxycephalosporin G by addition of catalaseQ Gao
Biology Department, Massachusetts Institute of Technology, Cambridge, MA, USA
Lett Appl Microbiol 34:290-2. 2002.... - Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal SL-protein reveals an unusual degree of internal homologyU T Gerngross
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
Mol Microbiol 8:325-34. 1993..thermocellum DNA and ruled out the possibility of rearrangements during the cloning and sequencing process. The sequenced gene is designated cipA and the encoded SL protein CipA...