A L Demain

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. ncbi request reprint Pharmaceutically active secondary metabolites of microorganisms
    A L Demain
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Appl Microbiol Biotechnol 52:455-63. 1999
  2. ncbi request reprint Induction of microbial secondary metabolism
    A L Demain
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Int Microbiol 1:259-64. 1998
  3. ncbi request reprint The natural functions of secondary metabolites
    A L Demain
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Adv Biochem Eng Biotechnol 69:1-39. 2000
  4. ncbi request reprint Secondary metabolism in simulated microgravity
    A L Demain
    Biology Department, Massachusetts Institute of Technology, Cambridge, USA
    Chem Rec 1:333-46. 2001
  5. ncbi request reprint Immobilized Streptomyces clavuligerus NP1 cells for biotransformation of penicillin G into deacetoxycephalosporin G
    A L Demain
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Appl Biochem Biotechnol 87:135-40. 2000
  6. ncbi request reprint Microbial biotechnology
    A L Demain
    Biology Department, Massachusetts Institute of Technology, Cambridge 02139, USA
    Trends Biotechnol 18:26-31. 2000
  7. ncbi request reprint The beta-lactam antibiotics: past, present, and future
    A L Demain
    Department of Biology, Massachusetts Intititute of Technology, Cambridge 02139, USA
    Antonie Van Leeuwenhoek 75:5-19. 1999
  8. ncbi request reprint Shear stress enhances microcin B17 production in a rotating wall bioreactor, but ethanol stress does not
    Q Gao
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Appl Microbiol Biotechnol 56:384-7. 2001
  9. ncbi request reprint Improvement in the bioconversion of penicillin G to deacetoxycephalosporin G by elimination of agitation and addition of decane
    Q Gao
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Appl Microbiol Biotechnol 57:511-3. 2001
  10. ncbi request reprint Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G
    Q Gao
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Ind Microbiol Biotechnol 30:190-4. 2003

Collaborators

  • R P Elander
  • Q Gao
  • A Fang
  • D F Gerson
  • J L Adrio
  • J M Piret
  • S K Mishra
  • D L Pierson
  • U T Gerngross
  • T Kobayashi
  • N S Huskisson
  • M P Romaniec

Detail Information

Publications17

  1. ncbi request reprint Pharmaceutically active secondary metabolites of microorganisms
    A L Demain
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Appl Microbiol Biotechnol 52:455-63. 1999
    ....
  2. ncbi request reprint Induction of microbial secondary metabolism
    A L Demain
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Int Microbiol 1:259-64. 1998
    ..The B-factor of A. mediterranei is an inducer of ansamycin (rifamycin) formation...
  3. ncbi request reprint The natural functions of secondary metabolites
    A L Demain
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Adv Biochem Eng Biotechnol 69:1-39. 2000
    ....
  4. ncbi request reprint Secondary metabolism in simulated microgravity
    A L Demain
    Biology Department, Massachusetts Institute of Technology, Cambridge, USA
    Chem Rec 1:333-46. 2001
    ..With respect to S. hygroscopicus, addition of Teflon beads to the RWB reversed the inhibition of growth, but rapamycin production was still markedly inhibited, and the distribution did not revert back to a preferential cellular site...
  5. ncbi request reprint Immobilized Streptomyces clavuligerus NP1 cells for biotransformation of penicillin G into deacetoxycephalosporin G
    A L Demain
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Appl Biochem Biotechnol 87:135-40. 2000
    ....
  6. ncbi request reprint Microbial biotechnology
    A L Demain
    Biology Department, Massachusetts Institute of Technology, Cambridge 02139, USA
    Trends Biotechnol 18:26-31. 2000
    ..Today, microbiology is a major participant in global industry, especially in the pharmaceutical, food and chemical industries...
  7. ncbi request reprint The beta-lactam antibiotics: past, present, and future
    A L Demain
    Department of Biology, Massachusetts Intititute of Technology, Cambridge 02139, USA
    Antonie Van Leeuwenhoek 75:5-19. 1999
    ..Although seventy years of age, the beta-lactams are not yet ready for retirement...
  8. ncbi request reprint Shear stress enhances microcin B17 production in a rotating wall bioreactor, but ethanol stress does not
    Q Gao
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Appl Microbiol Biotechnol 56:384-7. 2001
    ..Interestingly, cells growing in the bioreactor were much more resistant to the growth-inhibitory and production-inhibitory effects of ethanol than cells growing in shaken flasks...
  9. ncbi request reprint Improvement in the bioconversion of penicillin G to deacetoxycephalosporin G by elimination of agitation and addition of decane
    Q Gao
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA
    Appl Microbiol Biotechnol 57:511-3. 2001
    ..Many additives to the medium were examined for their effect on the reaction. Clearly, the most effective compound was the organic solvent, decane...
  10. ncbi request reprint Performance of a recombinant strain of Streptomyces lividans for bioconversion of penicillin G to deacetoxycephalosporin G
    Q Gao
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Ind Microbiol Biotechnol 30:190-4. 2003
    ..Whereas NP1 preferred 1.8 mM FeSO(4 )and 1.3 mM alpha-ketoglutarate, recombinant W25 performed best at 0.45 mM FeSO(4) and 1.9 mM alpha-ketoglutarate...
  11. ncbi request reprint Tetanus toxin production in soy-based medium: nutritional studies and scale-up into small fermentors
    A L Demain
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA
    Lett Appl Microbiol 45:635-8. 2007
    ..To further improve the soy-based medium, devoid of animal and dairy products, for a production of tetanus toxin by nutritional studies and to scale-up the Clostridium tetani process into small fermentors...
  12. ncbi request reprint Effect of solvents on bioconversion of penicillin G to deacetoxycephalosporin G
    Q Gao
    Biology Department, Massachusetts Institute of Technology, Cambridge 02139, USA
    J Antibiot (Tokyo) 54:958-61. 2001
    ..Optimum conditions of pH and temperature for the alkane system are not very different from the previously used aqueous system...
  13. ncbi request reprint Induction of microcin B17 formation in Escherichia coli ZK650 by limitation of oxygen and glucose is independent of glucose consumption rate
    Q Gao
    Fermentation Microbiology Laboratory, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Ind Microbiol Biotechnol 26:341-4. 2001
    ..0 mg/ml) under both high and low aeration. Glucose consumption rates were virtually identical under both high and low aeration. Thus, glucose consumption rate is not a regulating factor in microcin B17 formation...
  14. ncbi request reprint Molecular genetics and industrial microbiology--30 years of marriage
    A L Demain
    Biology Department, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Ind Microbiol Biotechnol 27:352-6. 2001
    ..Agricultural biotechnology has made great strides but unfortunately its progress is being delayed by political controversy...
  15. ncbi request reprint Menstrum for culture preservation and medium for seed preparation in a tetanus toxin production process containing no animal or dairy products
    A Fang
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA
    Lett Appl Microbiol 43:360-3. 2006
    ..To completely eliminate animal and dairy products from the lyophilization menstrum and the seed medium used to produce tetanus toxin with Clostridium tetani...
  16. ncbi request reprint Improvement in the resting-cell bioconversion of penicillin G to deacetoxycephalosporin G by addition of catalase
    Q Gao
    Biology Department, Massachusetts Institute of Technology, Cambridge, MA, USA
    Lett Appl Microbiol 34:290-2. 2002
    ..To improve the resting cell bioconversion of penicillin G to deacetoxycephalosporin G (DAOG) by elimination of an oxidizing intermediate which inactivates the enzyme during the reaction...
  17. ncbi request reprint Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal SL-protein reveals an unusual degree of internal homology
    U T Gerngross
    Department of Biology, Massachusetts Institute of Technology, Cambridge 02139
    Mol Microbiol 8:325-34. 1993
    ..thermocellum DNA and ruled out the possibility of rearrangements during the cloning and sequencing process. The sequenced gene is designated cipA and the encoded SL protein CipA...