Jianzhu Chen

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. pmc Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria
    Sebastian Leptihn
    Singapore MIT Alliance, Singapore
    BMC Biol 7:22. 2009
  2. ncbi request reprint A model T-cell receptor system for studying memory T-cell development
    Jianzhu Chen
    Center for Cancer Research and Department of Biology, E17 128, 40 Ames Street, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Microbes Infect 5:233-40. 2003
  3. pmc Genetic strategy to prevent influenza virus infections in animals
    Jianzhu Chen
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Infect Dis 197:S25-8. 2008
  4. pmc Endogenous T cell responses to antigens expressed in lung adenocarcinomas delay malignant tumor progression
    Michel DuPage
    Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cancer Cell 19:72-85. 2011
  5. pmc Cytosolic delivery mediated via electrostatic surface binding of protein, virus, or siRNA cargos to pH-responsive core-shell gel particles
    Yuhua Hu
    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Biomacromolecules 10:756-65. 2009
  6. ncbi request reprint Identification of novel superior polycationic vectors for gene delivery by high-throughput synthesis and screening of a combinatorial library
    Mini Thomas
    Department of Chemistry, Massachusetts Institute of Technology, Building 56 579, Cambridge, Massachusetts 02139, USA
    Pharm Res 24:1564-71. 2007
  7. pmc Antigen-bearing dendritic cells regulate the diverse pattern of memory CD8 T-cell development in different tissues
    Ching Hung Shen
    Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 107:22587-92. 2010
  8. pmc Competition for self-peptide-MHC complexes and cytokines between naive and memory CD8+ T cells expressing the same or different T cell receptors
    Qing Ge
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 101:3041-6. 2004
  9. pmc The T-cell receptor beta variable gene promoter is required for efficient V beta rearrangement but not allelic exclusion
    Chun Jeih Ryu
    Center for Cancer Research and Department of Biology, Massachusetts Institute for Technology, Cambridge, MA 02139, USA
    Mol Cell Biol 24:7015-23. 2004
  10. pmc Stage-dependent reactivity of thymocytes to self-peptide--MHC complexes
    Qibin Leng
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 104:5038-43. 2007

Research Grants

Collaborators

Detail Information

Publications79

  1. pmc Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria
    Sebastian Leptihn
    Singapore MIT Alliance, Singapore
    BMC Biol 7:22. 2009
    ..Despite the increasingly comprehensive understanding of the biochemical properties of these peptides, the actual mechanism by which antimicrobial peptides lyse microbes is controversial...
  2. ncbi request reprint A model T-cell receptor system for studying memory T-cell development
    Jianzhu Chen
    Center for Cancer Research and Department of Biology, E17 128, 40 Ames Street, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Microbes Infect 5:233-40. 2003
    ....
  3. pmc Genetic strategy to prevent influenza virus infections in animals
    Jianzhu Chen
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Infect Dis 197:S25-8. 2008
    ..Recent developments in RNA interference and transgenesis in birds should facilitate the development of influenza-resistant poultry...
  4. pmc Endogenous T cell responses to antigens expressed in lung adenocarcinomas delay malignant tumor progression
    Michel DuPage
    Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cancer Cell 19:72-85. 2011
    ..These results provide insight into the dynamic nature of the immune response to naturally arising tumors...
  5. pmc Cytosolic delivery mediated via electrostatic surface binding of protein, virus, or siRNA cargos to pH-responsive core-shell gel particles
    Yuhua Hu
    Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Biomacromolecules 10:756-65. 2009
    ..Functional gene knockdown following delivery of siRNA using the particles was demonstrated in epithelial cells. Based on these findings, these materials may be of interest for a broad range of biomedical applications...
  6. ncbi request reprint Identification of novel superior polycationic vectors for gene delivery by high-throughput synthesis and screening of a combinatorial library
    Mini Thomas
    Department of Chemistry, Massachusetts Institute of Technology, Building 56 579, Cambridge, Massachusetts 02139, USA
    Pharm Res 24:1564-71. 2007
    ....
  7. pmc Antigen-bearing dendritic cells regulate the diverse pattern of memory CD8 T-cell development in different tissues
    Ching Hung Shen
    Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 107:22587-92. 2010
    ..The findings have significant implications for design of vaccines and immunization strategies...
  8. pmc Competition for self-peptide-MHC complexes and cytokines between naive and memory CD8+ T cells expressing the same or different T cell receptors
    Qing Ge
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 101:3041-6. 2004
    ..These competitive relationships probably help maintain the size and TCR diversity of naïve and memory T cell populations required for optimal immune responses...
  9. pmc The T-cell receptor beta variable gene promoter is required for efficient V beta rearrangement but not allelic exclusion
    Chun Jeih Ryu
    Center for Cancer Research and Department of Biology, Massachusetts Institute for Technology, Cambridge, MA 02139, USA
    Mol Cell Biol 24:7015-23. 2004
    ..Although the promoter is dispensable for allelic exclusion, it appears to suppress aberrant V beta cleavages during allelic exclusion...
  10. pmc Stage-dependent reactivity of thymocytes to self-peptide--MHC complexes
    Qibin Leng
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 104:5038-43. 2007
    ....
  11. pmc B cell development and immunoglobulin transcription in Oct-1-deficient mice
    Victoria E H Wang
    Department of Biology and Center for Cancer Research and McGovern Institute, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139 4307, USA
    Proc Natl Acad Sci U S A 101:2005-10. 2004
    ..However, recipient mice show slight reductions in serum IgM and IgA. Thus, the Oct-1 protein is dispensable for B cell development and Ig transcription...
  12. pmc Attaching zanamivir to a polymer markedly enhances its activity against drug-resistant strains of influenza a virus
    Alisha K Weight
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Pharm Sci 100:831-5. 2011
    ..These findings provide a basis for exploring polymer-attached inhibitors as more efficacious therapeutics, particularly against drug-resistant influenza strains...
  13. ncbi request reprint Block of T cell development in P53-deficient mice accelerates development of lymphomas with characteristic RAG-dependent cytogenetic alterations
    Brian B Haines
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Cancer Cell 9:109-20. 2006
    ..These findings provide genetic evidence that block of lymphocyte development at stages with RAG endonuclease activity can provoke lymphomagenesis on a background with deficient DNA damage responses...
  14. pmc Inhibition of influenza virus production in virus-infected mice by RNA interference
    Qing Ge
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 101:8676-81. 2004
    ..Development of delivery systems that may be compatible with human use demonstrates the potential utility of siRNAs for prophylaxis and therapy of influenza virus infections in humans...
  15. pmc Bifunctional polymeric inhibitors of human influenza A viruses
    Jayanta Haldar
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Pharm Res 27:259-63. 2010
    ....
  16. pmc Polymer-attached zanamivir inhibits synergistically both early and late stages of influenza virus infection
    Chia Min Lee
    Computational and Systems Biology Initiative, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 109:20385-90. 2012
    ..These findings both rationalize the great anti-influenza potency of PGN-ZA and reveal that attaching ZA to a polymeric chain confers a unique mechanism of antiviral action potentially useful for minimizing drug resistance...
  17. doi request reprint Differential requirement for CD70 and CD80/CD86 in dendritic cell-mediated activation of tumor-tolerized CD8 T cells
    S Peter Bak
    Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Immunol 189:1708-16. 2012
    ..These findings reveal dynamic requirements for costimulatory signals to overcome tumor-induced tolerance and have significant implications for developing more effective cancer immunotherapies...
  18. pmc Mesenchymal stem cells secreting angiopoietin-like-5 support efficient expansion of human hematopoietic stem cells without compromising their repopulating potential
    Maroun Khoury
    Department of Biology, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Stem Cells Dev 20:1371-81. 2011
    ..Further development of this coculture method for expanding human HSCs for clinical and preclinical applications is therefore warranted...
  19. pmc Signaling thresholds govern heterogeneity in IL-7-receptor-mediated responses of naïve CD8(+) T cells
    Megan J Palmer
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Immunol Cell Biol 89:581-94. 2011
    ..Our findings indicate that IL-7R-mediated signaling not only maintains the size but also impacts the diversity of the naïve T-cell repertoire...
  20. pmc Persistence of tumor-infiltrating CD8 T cells is tumor-dependent but antigen-independent
    Mobolaji O Olurinde
    Harvard MIT Division of Health Sciences and Technology, Cambridge, MA, USA
    Cell Mol Immunol 8:415-23. 2011
    ..These results also allow CD8 T-cell survival in the tumor environment to be compared with T-cell survival in chronic infection...
  21. ncbi request reprint Recombination activating genes (RAG) in lymphoma development
    Brian B Haines
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Cell Cycle 5:913-6. 2006
    ..Our model system provides a means to assess the roles of RAG activity in human lymphoid malignancies...
  22. pmc Different contributions of thymopoiesis and homeostasis-driven proliferation to the reconstitution of naive and memory T cell compartments
    Qing Ge
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:2989-94. 2002
    ..After severe T cell depletion homeostasis-driven proliferation restores only the memory T cell compartment, whereas thymopoiesis is required for the reconstitution of the naive T cell compartment...
  23. pmc Expression of multiple artificial microRNAs from a chicken miRNA126-based lentiviral vector
    Steve C Y Chen
    Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 6:e22437. 2011
    ..The use of RNAi in both basic and translational research often requires expression of multiple siRNAs from the same vector...
  24. pmc Cutting edge: delay and reversal of T cell tolerance by intratumoral injection of antigen-loaded dendritic cells in an autochthonous tumor model
    Eileen M Higham
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Immunol 184:5954-8. 2010
    ..They also suggest new potential strategies to extend the functionality of transferred effector T cells and to restore function to tolerized tumor-infiltrating T cells for cancer immunotherapy...
  25. pmc Poly-beta amino ester-containing microparticles enhance the activity of nonviral genetic vaccines
    Steven R Little
    Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 101:9534-9. 2004
    ..When used as vaccines in vivo, these microparticle formulations, unlike conventional formulations, induce antigen-specific rejection of transplanted syngenic tumor cells...
  26. pmc Promiscuous binding of extracellular peptides to cell surface class I MHC protein
    Herman N Eisen
    Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 109:4580-5. 2012
    ....
  27. pmc Loss of IL-7R and IL-15R expression is associated with disappearance of memory T cells in respiratory tract following influenza infection
    Ching Hung Shen
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Immunol 180:171-8. 2008
    ..These findings provide a molecular explanation for the gradual loss of airway memory CD8 T cells and heterosubtypic immunity following influenza infection...
  28. pmc Activation of tolerogenic dendritic cells in the tumor draining lymph nodes by CD8+ T cells engineered to express CD40 ligand
    Eileen M Higham
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Immunol 184:3394-400. 2010
    ..These findings demonstrate that augmented T cell responses can be achieved by engineering tumor-reactive T cells to deliver stimulatory signals to dendritic cells in the tumor microenvironment...
  29. pmc B7-H1 (PD-L1) on T cells is required for T-cell-mediated conditioning of dendritic cell maturation
    Oezcan Talay
    Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 106:2741-6. 2009
    ..In return, the mature DCs stimulate a robust T-cell response against the infecting pathogen...
  30. doi request reprint Conjugating drug candidates to polymeric chains does not necessarily enhance anti-influenza activity
    Alyssa M Larson
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Pharm Sci 101:3896-905. 2012
    ..These observations, further explored by means of molecular modeling, reveal the previously unrecognized unpredictability of the benefits of multivalency, possibly because of poor accessibility of the viral targets to polymeric agents...
  31. ncbi request reprint Cross-linked small polyethylenimines: while still nontoxic, deliver DNA efficiently to mammalian cells in vitro and in vivo
    Mini Thomas
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Pharm Res 22:373-80. 2005
    ....
  32. ncbi request reprint Polycation-mediated delivery of siRNAs for prophylaxis and treatment of influenza virus infection
    Mini Thomas
    Division of Biological Engineering, Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Expert Opin Biol Ther 5:495-505. 2005
    ....
  33. pmc Development of CD4+ T cells expressing a nominally MHC class I-restricted T cell receptor by two different mechanisms
    Qing Ge
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 103:1822-7. 2006
    ....
  34. pmc Elevated CXCL12 expression in the bone marrow of NOD mice is associated with altered T cell and stem cell trafficking and diabetes development
    Qibin Leng
    Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    BMC Immunol 9:51. 2008
    ..Despite these initial observations, however, how CXCL12 affects development of TID has not been fully investigated...
  35. pmc Drug delivery-mediated control of RNA immunostimulation
    David N Nguyen
    Division of Health Sciences and Technology, MIT, Cambridge, Massachusetts 02142, USA
    Mol Ther 17:1555-62. 2009
    ..Here, we demonstrate that innate immune activation caused by unmodified siRNA can have therapeutically relevant effects, and that these non-RNAi effects can be controlled through chemical modifications and drug delivery...
  36. ncbi request reprint Non-viral siRNA delivery to the lung
    Mini Thomas
    Department of Chemistry and Division of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Adv Drug Deliv Rev 59:124-33. 2007
    ..This review focuses on the recent progress in non-viral delivery of siRNAs for the treatment of lung diseases, particularly infectious diseases. The rapid progress will put siRNA-based therapeutics on fast track to the clinic...
  37. pmc Soluble peptide-MHC monomers cause activation of CD8+ T cells through transfer of the peptide to T cell MHC molecules
    Qing Ge
    Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:13729-34. 2002
    ..The result is that T cells, acting as antigen-presenting cells, are able to activate other naive T cells...
  38. ncbi request reprint CD4+ T-cell responses to self-peptide--MHC
    Qing Ge
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Trends Immunol 24:186-9. 2003
    ..One explanation for these seemingly disparate results could be the use of mice that were assumed to be MHC class II null but might express some uncommon MHC class II heterodimers...
  39. ncbi request reprint Naïve to memory T-cell differentiation during homeostasis-driven proliferation
    Qing Ge
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, E17 128, 40 Ames Street, Cambridge, MA 02139, USA
    Microbes Infect 4:555-8. 2002
    ....
  40. doi request reprint Engineering humanized mice for improved hematopoietic reconstitution
    Adam C Drake
    Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA
    Cell Mol Immunol 9:215-24. 2012
    ..We highlight the opportunities created by new technologies and discuss practical considerations on how to make best use of the widening array of basic models for specific research applications...
  41. pmc Synapse-directed delivery of immunomodulators using T-cell-conjugated nanoparticles
    Matthias T Stephan
    Department of Material Science and Engineering, Massachusetts Institute of Technology MIT, Cambridge, MA 02139, USA
    Biomaterials 33:5776-87. 2012
    ....
  42. pmc Lipid-derived nanoparticles for immunostimulatory RNA adjuvant delivery
    David N Nguyen
    Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    Proc Natl Acad Sci U S A 109:E797-803. 2012
    ....
  43. pmc Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung
    Mini Thomas
    Department of Chemistry and Division of Biological Engineering, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 102:5679-84. 2005
    ..Furthermore, a polyplex of a siRNA against the influenza viral nucleocapsid protein gene and PEI87 resulted in a 94% drop of virus titers in the lungs of influenza-infected animals...
  44. pmc Regulated expression of a tumor-associated antigen reveals multiple levels of T-cell tolerance in a mouse model of lung cancer
    Ann F Cheung
    Koch Institute and Department of Biology, Cambridge, Massachusetts, USA
    Cancer Res 68:9459-68. 2008
    ..This system represents a valuable tool in which to explore sources contributing to T-cell tolerance of cancer and to test therapies aimed at overcoming this tolerance...
  45. pmc Rapid tolerization of virus-activated tumor-specific CD8+ T cells in prostate tumors of TRAMP mice
    Ailin Bai
    Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 105:13003-8. 2008
    ..Our model provides a system to study T cell-tumor interactions in detail and to test the efficacy of cancer immunotherapeutic strategies...
  46. pmc RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription
    Qing Ge
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 100:2718-23. 2003
    ..The findings reveal that newly synthesized NP and PA proteins are required for influenza virus transcription and replication and provide a basis for the development of siRNAs as prophylaxis and therapy for influenza infection in humans...
  47. ncbi request reprint A peptide that antagonizes TCR-mediated reactions with both syngeneic and allogeneic agonists: functional and structural aspects
    Markus G Rudolph
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Immunol 172:2994-3002. 2004
    ....
  48. pmc The T cell receptor beta enhancer promotes access and pairing of Dbeta and Jbeta gene segments during V(D)J recombination
    Chun Jeih Ryu
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 100:13465-70. 2003
    ....
  49. pmc A novel mechanism is involved in cationic lipid-mediated functional siRNA delivery
    James J Lu
    Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Mol Pharm 6:763-71. 2009
    ..Our findings suggest possible directions for improving functional siRNA delivery by cationic lipids...
  50. ncbi request reprint T cell proliferation, differentiation, and restoration in lymphopenic individuals: for contributed volumes
    Jianzhu Chen
    Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Adv Exp Med Biol 512:135-9. 2002
  51. pmc Hydrophobic polycationic coatings disinfect poliovirus and rotavirus solutions
    Alyssa M Larson
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Biotechnol Bioeng 108:720-3. 2011
    ....
  52. pmc Inhibition of IFN-gamma transcription by site-specific methylation during T helper cell development
    Brendan Jones
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    EMBO J 25:2443-52. 2006
    ..These findings suggest that rapid methylation of the evolutionarily conserved -53 CpG by Dnmt3a may suppress IFN-gamma transcription in developing TH2 cells by directly inhibiting transcription factor binding...
  53. ncbi request reprint Kruppel-like factor 2 controls T cell trafficking by activating L-selectin (CD62L) and sphingosine-1-phosphate receptor 1 transcription
    Ailin Bai
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 40 Ames Street, Cambridge, MA 02139, USA
    J Immunol 178:7632-9. 2007
    ..These findings suggest that KLF2 regulates T cell homeostasis at least partly by controlling CD62L and S1P1 expression, and therefore T cell egress from the thymus and circulation in the periphery...
  54. ncbi request reprint Hydrophobic polycationic coatings inactivate wild-type and zanamivir- and/or oseltamivir-resistant human and avian influenza viruses
    Jayanta Haldar
    Department of Chemistry and Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biotechnol Lett 30:475-9. 2008
    ....
  55. doi request reprint Graphene multilayers as gates for multi-week sequential release of proteins from surfaces
    Jinkee Hong
    Department of Chemical Engineering, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    ACS Nano 6:81-8. 2012
    ..This approach provides a new route for storage of therapeutics in a solid-state thin film for subsequent delivery in a time-controlled and sequential fashion...
  56. ncbi request reprint Use of siRNAs to prevent and treat influenza virus infection
    Qing Ge
    Department of Biology, Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Virus Res 102:37-42. 2004
    ..In this review, we discuss the potential value of siRNAs for preventing and treating influenza virus infections in humans and the challenges that have to be overcome to realize their potential...
  57. ncbi request reprint Molecularly engineered poly(ortho ester) microspheres for enhanced delivery of DNA vaccines
    Chun Wang
    Department of Chemical Engineering, E25 342, 77 Mass Ave, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Nat Mater 3:190-6. 2004
    ..This polymer microparticulate system could, with further study, have implications for advancing the clinical utility of DNA vaccines as well as other nucleic-acid-based therapeutics against viral infections and cancer...
  58. pmc Mechanism of inactivation of influenza viruses by immobilized hydrophobic polycations
    Bryan B Hsu
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 108:61-6. 2011
    ....
  59. ncbi request reprint Small interfering RNA-mediated gene silencing in T lymphocytes
    Michael T McManus
    Center for Cancer Research, Massachusetts Institute of Technology, 40 Ames Street E17 526, Cambridge, MA 02139, USA
    J Immunol 169:5754-60. 2002
    ..Using primary differentiated T lymphocytes, we provide the first evidence of siRNA-mediated RNAi gene silencing in normal nontransformed somatic mammalian lymphocytes...
  60. ncbi request reprint A B cell-based sensor for rapid identification of pathogens
    Todd H Rider
    Massachusetts Institute of Technology Lincoln Laboratory, Lexington, MA 02420, USA
    Science 301:213-5. 2003
    ..Because of its speed, sensitivity, and specificity, this pathogen identification technology could prove useful for medical diagnostics, biowarfare defense, food- and water-quality monitoring, and other applications...
  61. pmc Polymeric coatings that inactivate both influenza virus and pathogenic bacteria
    Jayanta Haldar
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 103:17667-71. 2006
    ..A relationship between the structure of the derivatized PEI and the resultant virucidal activity of the painted surface has been elucidated...
  62. pmc Interleukin-7 receptor signaling network: an integrated systems perspective
    Megan J Palmer
    Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell Mol Immunol 5:79-89. 2008
    ..Analysis of IL-7 receptor signaling at a network level in a systematic manner will allow for a comprehensive approach to understanding the impact of multiple signaling pathways on lymphocyte biology...
  63. pmc Virus-specific host miRNAs: antiviral defenses or promoters of persistent infection?
    Vinay S Mahajan
    Department of Biological Engineering, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Trends Immunol 30:1-7. 2009
    ..The presence of host-miRNA targets in the genomes of rapidly evolving viruses probably reflects the adaptation of these viruses to the cellular miRNA milieu...
  64. pmc Homeostatic T cell proliferation in a T cell-dendritic cell coculture system
    Qing Ge
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:2983-8. 2002
    ..The coculture system described may illuminate mechanisms that maintain stable numbers of T cells in normal individuals...
  65. pmc Human CD34+ CD133+ hematopoietic stem cells cultured with growth factors including Angptl5 efficiently engraft adult NOD-SCID Il2rγ-/- (NSG) mice
    Adam C Drake
    Koch Institute for Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS ONE 6:e18382. 2011
    ..The ability to expand human HSCs in vitro should facilitate clinical use of HSCs and large-scale construction of humanized mice from the same donor for research applications...
  66. pmc Densely interconnected transcriptional circuits control cell states in human hematopoiesis
    Noa Novershtern
    Broad Institute, Cambridge, MA 02142, USA
    Cell 144:296-309. 2011
    ..These findings suggest a more complex regulatory system for hematopoiesis than previously assumed...
  67. pmc Embryonic lethality, decreased erythropoiesis, and defective octamer-dependent promoter activation in Oct-1-deficient mice
    Victoria E H Wang
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 4307, USA
    Mol Cell Biol 24:1022-32. 2004
    ..These findings suggest a critical role for Oct-1 during development and a stringent gene dosage effect with Oct-2 in mediating postnatal survival...
  68. doi request reprint Removing human immunodeficiency virus (HIV) from human blood using immobilized heparin
    Roger A Nassar
    Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Biotechnol Lett 34:853-6. 2012
    ..The detoxification concept has been validated by both circulating an HIV-1 solution through a column packed with the heparin-sepharose beads and successively mixing an HIV-1 solution with fresh beads...
  69. ncbi request reprint How do cultured CD8(+) murine T cell clones survive repeated ligation of the TCR?
    Satoshi Sugawa
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Int Immunol 14:23-30. 2002
    ....
  70. pmc Gene silencing using micro-RNA designed hairpins
    Michael T McManus
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02139, USA
    RNA 8:842-50. 2002
    ..Furthermore, these RNAs are active when expressed by DNA vectors containing polymerase III promoters, opening the possibility for new approaches in stable RNAi-based loss of function studies...
  71. ncbi request reprint Hypoxia-inducible factor 1alpha-deficient chimeric mice as a model to study abnormal B lymphocyte development and autoimmunity
    Hidefumi Kojima
    Division on Immunology, Institute for Medical Science, Dokkyo University School of Medicine, Tochigi, Japan
    Methods Enzymol 381:218-29. 2004
  72. ncbi request reprint The resolution of relapsing fever borreliosis requires IgM and is concurrent with expansion of B1b lymphocytes
    Kishore R Alugupalli
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA
    J Immunol 170:3819-27. 2003
    ..hermsii. Together these results support the model that B1b cells generate the T-independent IgM required for the control and resolution of relapsing fever borreliosis...
  73. ncbi request reprint Regulation of TCRbeta gene assembly by a promoter/enhancer holocomplex
    Kenneth J Oestreich
    Department of Microbiology and Immunology, Vanderbilt University, Nashville, Tennessee 37232, USA
    Immunity 24:381-91. 2006
    ....
  74. ncbi request reprint Introduction: regulation of lymphocyte homeostasis
    Antonio Freitas
    Biologie des Populations Lymphocytaires, CNRS 1961, Departement d Immunologie, Institut Pasteur, 25, rue du Dr Roux, 75724, Paris, France
    Microbes Infect 4:529-30. 2002
  75. ncbi request reprint Anti-SARS-CoV immunity induced by a novel CpG oligodeoxynucleotide
    Musheng Bao
    Department of Molecular Biology, College of Basic Medicine, Jilin University, Changchun 130021, China
    Clin Immunol 118:180-7. 2006
    ..Together, BW001 represents a novel type of CpG ODN and may have potential for the development of treatment and prevention for SARS as well as other viral associated diseases...
  76. pmc Central importance of immunoglobulin A in host defense against Giardia spp
    T Dianne Langford
    Department of Medicine, University of California, San Diego, La Jolla, California 92093 0623, USA
    Infect Immun 70:11-8. 2002
    ..Moreover, B-cell-dependent but IgA-independent and B-cell-independent antigiardial host defenses exist but are less important for controlling infection...
  77. ncbi request reprint Expression of Gal4-VP16 and Gal4-DNA binding domain under the control of the T lymphocyte-specific lck proximal promoter in transgenic mice
    Chun Jeih Ryu
    Institute of Bioscience, Department of Bioscience and Biotechnology, Sejong University, 98 Gunja Dong, Gwangjin gu, Seoul 143 747, Korea
    BMB Rep 41:575-80. 2008
    ....
  78. ncbi request reprint The presence of MOMA-2+ macrophages in the outer B cell zone and protection of the splenic micro-architecture from LPS-induced destruction depend on secreted IgM
    Michael B Fischer
    Department of Transfusion Medicine, Medical University of Vienna, Vienna, Austria
    Eur J Immunol 37:2825-33. 2007
    ..The consequence was a massive destruction of the microarchitecture of the spleen where marginal zones disorganized, lymphoid follicles and T cell zones disrupted and follicular DC (FDC) networks disappeared...
  79. ncbi request reprint T-cell engagement of dendritic cells rapidly rearranges MHC class II transport
    Marianne Boes
    Department of Pathology, Harvard Medical School, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Nature 418:983-8. 2002
    ..We propose that such tubulation serves to facilitate the ensuing T-cell response...

Research Grants38

  1. Control of V(D)J Recombination at the TCR beta Locus
    Jianzhu Chen; Fiscal Year: 2006
    ..The proposed studies may help elucidate the role of enhancers in regulating DSB repair, V(D)J recombination, genome instability, and tumorigenesis. ..
  2. RNA Interference of Influenza Virus Infection
    Jianzhu Chen; Fiscal Year: 2006
    ..Theoretically, an siRNA-based treatment should remain effective even with the emergence of new strains of the virus. ..
  3. Regulation of T Cell Homeostasis
    Jianzhu Chen; Fiscal Year: 2006
    ..In addition, the role of cell division in facilitating changes in chromatin structure at the IFN-g locus will be investigated by measuring the extent of DNA methylation, histone acetylation and factor binding...
  4. Control of V(D)J Recombination at the TCR beta Locus
    Jianzhu Chen; Fiscal Year: 2007
    ..The proposed studies may help elucidate the role of enhancers in regulating DSB repair, V(D)J recombination, genome instability, and tumorigenesis. ..
  5. Mechanisms of T Cell Tumorigenesis
    Jianzhu Chen; Fiscal Year: 2007
    ..Findings from the proposed studies may shed light on the etiology and mechanisms underlying the frequent translocations of the antigen receptor loci in lymphoid tumors in humans. ..
  6. Development and Maintenance of Memory CD8 T Cells
    Jianzhu Chen; Fiscal Year: 2007
    ....
  7. Bi-functional Polymer-Attached Inhibitors of Influenza Viruses
    Jianzhu Chen; Fiscal Year: 2007
    ..that the proposed research will (i) yield one or more highly potent, optimized polymer-attached inhibitor(s) for future clinical development and (ii) provide a new paradigm of drug development for overcoming microbial drug resistance ..
  8. CIS REGULATORY ELEMENTS FOR TCRB GENE ALLELIC EXCLUSION
    Jianzhu Chen; Fiscal Year: 2001
    ..An understanding of the control of allelic exclusion at the molecular level may help to elucidate mechanisms that underlie immunodeficiencies and autoimmune diseases. ..
  9. TRANSGENIC MOUSE MODEL FOR ANALYZING T CELL RESPONSES
    Jianzhu Chen; Fiscal Year: 2002
    ....
  10. Control of V(D)J Recombination at the TCR beta Locus
    Jianzhu Chen; Fiscal Year: 2003
    ..The proposed studies may help elucidate the role of enhancers in regulating DSB repair, V(D)J recombination, genome instability, and tumorigenesis. ..
  11. Development and Maintenance of Memory CD8 T Cells
    Jianzhu Chen; Fiscal Year: 2010
    ....