Stephen P Bell

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. pmc Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading
    Shuyan Chen
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 21:2897-907. 2007
  2. pmc Separation of DNA replication from the assembly of break-competent meiotic chromosomes
    Hannah G Blitzblau
    Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS Genet 8:e1002643. 2012
  3. ncbi request reprint The origin recognition complex: from simple origins to complex functions
    Stephen P Bell
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 16:659-72. 2002
  4. ncbi request reprint DNA replication in eukaryotic cells
    Stephen P Bell
    Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA
    Annu Rev Biochem 71:333-74. 2002
  5. pmc Multiple Cdt1 molecules act at each origin to load replication-competent Mcm2-7 helicases
    Thomas J Takara
    Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, USA
    EMBO J 30:4885-96. 2011
  6. pmc Incorporation into the prereplicative complex activates the Mcm2-7 helicase for Cdc7-Dbf4 phosphorylation
    Laura I Francis
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 23:643-54. 2009
  7. pmc Mec1 is one of multiple kinases that prime the Mcm2-7 helicase for phosphorylation by Cdc7
    John C W Randell
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 40:353-63. 2010
  8. ncbi request reprint Sequential ATP hydrolysis by Cdc6 and ORC directs loading of the Mcm2-7 helicase
    John C W Randell
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Mol Cell 21:29-39. 2006
  9. pmc CDK prevents Mcm2-7 helicase loading by inhibiting Cdt1 interaction with Orc6
    Shuyan Chen
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 25:363-72. 2011
  10. pmc Eukaryotic origin-dependent DNA replication in vitro reveals sequential action of DDK and S-CDK kinases
    Ryan C Heller
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Cell 146:80-91. 2011

Collaborators

Detail Information

Publications28

  1. pmc Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading
    Shuyan Chen
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 21:2897-907. 2007
    ..Interestingly, this complex can only perform a single round of Mcm2-7 loading, suggesting that a dynamic association of Cdt1 with ORC is required for multiple rounds of Mcm2-7 loading...
  2. pmc Separation of DNA replication from the assembly of break-competent meiotic chromosomes
    Hannah G Blitzblau
    Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts, United States of America
    PLoS Genet 8:e1002643. 2012
    ..The functional separation of these processes reveals a modular method of building meiotic chromosomes and predicts that any crosstalk between these modules must occur through superimposed regulatory mechanisms...
  3. ncbi request reprint The origin recognition complex: from simple origins to complex functions
    Stephen P Bell
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 16:659-72. 2002
  4. ncbi request reprint DNA replication in eukaryotic cells
    Stephen P Bell
    Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA
    Annu Rev Biochem 71:333-74. 2002
    ..We emphasize recent progress in determining the function of the different replication factors once they have been assembled at the origin...
  5. pmc Multiple Cdt1 molecules act at each origin to load replication-competent Mcm2-7 helicases
    Thomas J Takara
    Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA, USA
    EMBO J 30:4885-96. 2011
    ....
  6. pmc Incorporation into the prereplicative complex activates the Mcm2-7 helicase for Cdc7-Dbf4 phosphorylation
    Laura I Francis
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 23:643-54. 2009
    ....
  7. pmc Mec1 is one of multiple kinases that prime the Mcm2-7 helicase for phosphorylation by Cdc7
    John C W Randell
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 40:353-63. 2010
    ..These findings identify regulatory mechanisms that modulate origin firing and replication fork assembly during cell cycle progression...
  8. ncbi request reprint Sequential ATP hydrolysis by Cdc6 and ORC directs loading of the Mcm2-7 helicase
    John C W Randell
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Mol Cell 21:29-39. 2006
    ..Importantly, these coordinated yet distinct functions of ORC and Cdc6 ensure the correct temporal and spatial regulation of pre-RC formation...
  9. pmc CDK prevents Mcm2-7 helicase loading by inhibiting Cdt1 interaction with Orc6
    Shuyan Chen
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 25:363-72. 2011
    ....
  10. pmc Eukaryotic origin-dependent DNA replication in vitro reveals sequential action of DDK and S-CDK kinases
    Ryan C Heller
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Cell 146:80-91. 2011
    ..Our studies identify distinct roles for DDK and S-CDK during helicase activation and support a model in which the leading strand DNA polymerase is recruited prior to origin DNA unwinding and RNA primer synthesis...
  11. pmc Dynamics of pre-replicative complex assembly
    Vasiliki Tsakraklides
    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    J Biol Chem 285:9437-43. 2010
    ..This dissociation requires ATP hydrolysis at a late stage of pre-RC assembly. Our results indicate that pre-RC formation is a dynamic process...
  12. pmc The B2 element of the Saccharomyces cerevisiae ARS1 origin of replication requires specific sequences to facilitate pre-RC formation
    Gwendolyn M Wilmes
    Department of Biology and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:101-6. 2002
    ..The function of these mutant sequences is rescued by Cdc6p overexpression. We propose that the B2 element requires specific sequences to bind a component of the pre-RC...
  13. pmc Genome-wide analysis of re-replication reveals inhibitory controls that target multiple stages of replication initiation
    Robyn E Tanny
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Biol Cell 17:2415-23. 2006
    ..Our findings allow us to categorize origins with respect to their propensity to reinitiate and demonstrate that pre-RC formation is not the only target for the mechanisms that prevent genomic re-replication...
  14. pmc Interaction of the S-phase cyclin Clb5 with an "RXL" docking sequence in the initiator protein Orc6 provides an origin-localized replication control switch
    Gwendolyn M Wilmes
    Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Genes Dev 18:981-91. 2004
    ..We propose that Clb5 binding to ORC provides an origin-localized replication control switch that specifically prevents reinitiation at replicated origins...
  15. doi request reprint Putting two heads together to unwind DNA
    Thomas J Takara
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Cell 139:652-4. 2009
    ..Remus et al. (2009) provide important insights into helicase loading in eukaryotes, showing that the Mcm2-7 replicative helicase encircles double-stranded DNA as head-to-head double hexamers...
  16. pmc Coordination of replication and transcription along a Drosophila chromosome
    David M MacAlpine
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02319, USA
    Genes Dev 18:3094-105. 2004
    ..Based on these findings, we suggest that the distribution of transcription along the chromosome acts locally to influence origin selection and globally to regulate origin activation...
  17. ncbi request reprint ATP hydrolysis by ORC catalyzes reiterative Mcm2-7 assembly at a defined origin of replication
    Jayson L Bowers
    Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Mol Cell 16:967-78. 2004
    ....
  18. ncbi request reprint Mapping of meiotic single-stranded DNA reveals double-stranded-break hotspots near centromeres and telomeres
    Hannah G Blitzblau
    Department of Biology, Howard Hughes Medical Institute, Cambridge, Massachusetts 02139, USA
    Curr Biol 17:2003-12. 2007
    ..At least two levels of regulation govern crossover distribution: where the initiating DNA double-strand breaks (DSBs) occur and whether those DSBs are repaired as crossovers...
  19. pmc Cell-cycle control of the establishment of mating-type silencing in S. cerevisiae
    Anna Lau
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 16:2935-45. 2002
    ..Our findings reveal both S- and M-phase requirements for the establishment of silencing and implicate the loss of sister-chromatid cohesion as a critical event in this process...
  20. pmc Visualization of replication initiation and elongation in Drosophila
    Julie M Claycomb
    Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA
    J Cell Biol 159:225-36. 2002
    ....
  21. ncbi request reprint A genomic view of eukaryotic DNA replication
    David M MacAlpine
    Howard Hughes Medical Institute, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Chromosome Res 13:309-26. 2005
    ..In addition to the insights they have provided already, microarray-based replication assays combined with genetic analysis will provide a powerful new approach to define the mechanisms that regulate replication origin function...
  22. pmc The histone modification pattern of active genes revealed through genome-wide chromatin analysis of a higher eukaryote
    Dirk Schübeler
    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA
    Genes Dev 18:1263-71. 2004
    ..Furthermore, the degree of modification correlates with the level of transcription, and modifications are largely restricted to transcribed regions, suggesting that their regulation is tightly linked to polymerase activity...
  23. ncbi request reprint Mapping subunit location on the Saccharomyces cerevisiae origin recognition complex free and bound to DNA using a novel nanoscale biopointer
    Paul D Chastain
    Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC 27599 7295, USA
    J Biol Chem 279:36354-62. 2004
    ..This versatile, new approach to mapping protein structure has potential for many applications...
  24. ncbi request reprint Cell cycle execution point analysis of ORC function and characterization of the checkpoint response to ORC inactivation in Saccharomyces cerevisiae
    Daniel G Gibson
    Molecular and Computational Biology Program, Department of Biological Sciences, University of Southern California, Los Angeles, California 90089 2910, USA
    Genes Cells 11:557-73. 2006
    ..We discuss the potential significance of these overlapping checkpoints and the impact of our findings on previously postulated role(s) of ORCs in other cell cycle functions...
  25. pmc Subunit organization of Mcm2-7 and the unequal role of active sites in ATP hydrolysis and viability
    Matthew L Bochman
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    Mol Cell Biol 28:5865-73. 2008
    ..Our conclusions predict a structural discontinuity between Mcm2 and Mcm5 and demonstrate that in contrast to other hexameric helicases, the six Mcm2-7 active sites are functionally distinct...
  26. pmc Localized H3K36 methylation states define histone H4K16 acetylation during transcriptional elongation in Drosophila
    Oliver Bell
    Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse, Basel, Switzerland
    EMBO J 26:4974-84. 2007
    ..Thus di- and trimethylation of H3K36 have opposite effects on H4K16 acetylation, which we propose enable dynamic changes in chromatin compaction during transcript elongation...
  27. pmc Genomic profiling and expression studies reveal both positive and negative activities for the Drosophila Myb MuvB/dREAM complex in proliferating cells
    Daphne Georlette
    Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California 94720, USA
    Genes Dev 21:2880-96. 2007
    ....

Research Grants22

  1. INITIATION OF DNA REPLICATION OF YEAST CHROMOSOMES
    Stephen P Bell; Fiscal Year: 2010
    ..The findings will provide fundamentally important information that will be relevant to the proliferation of all animal cells including humans. ..
  2. INITIATION OF DNA REPLICATION OF YEAST CHROMOSOMES
    Stephen Bell; Fiscal Year: 2003
    ....
  3. INITIATION OF DNA REPLICATION OF YEAST CHROMOSOMES
    Stephen Bell; Fiscal Year: 2004
    ....
  4. INITIATION OF DNA REPLICATION OF YEAST CHROMOSOMES
    Stephen Bell; Fiscal Year: 2005
    ....
  5. INITIATION OF DNA REPLICATION OF YEAST CHROMOSOMES
    Stephen Bell; Fiscal Year: 2006
    ....
  6. INITIATION OF DNA REPLICATION OF YEAST CHROMOSOMES
    Stephen Bell; Fiscal Year: 2007
    ....
  7. Pre-Doctoral Grant in the Biological Sciences
    Stephen Bell; Fiscal Year: 2007
    ..Our graduate students play major roles in the intellectual life and research efforts of the Department and go on to make substantive contributions to modern biology. ..
  8. INITIATION OF DNA REPLICATION OF YEAST CHROMOSOMES
    Stephen Bell; Fiscal Year: 2009
    ..The findings will provide fundamentally important information that will be relevant to the proliferation of all animal cells including humans. ..
  9. INITIATION OF DNA REPLICATION OF YEAST CHROMOSOMES
    Stephen Bell; Fiscal Year: 2002
    ....
  10. MCM PROTEIN FUNCTION DURING EUKARYOTIC DNA REPLICATION
    Stephen Bell; Fiscal Year: 2002
    ....
  11. MCM PROTEIN FUNCTION DURING EUKARYOTIC DNA REPLICATION
    Stephen Bell; Fiscal Year: 1999
    ....
  12. INITIATION OF DNA REPLICATION OF YEAST CHROMOSOMES
    Stephen Bell; Fiscal Year: 1999
    ..Second, an understanding DNA replication and its regulation in yeast will provide new targets for the development of anti-fungal drugs and therapies. ..
  13. INITIATION OF DNA REPLICATION OF YEAST CHROMOSOMES
    Stephen Bell; Fiscal Year: 2000
    ....
  14. MCM PROTEIN FUNCTION DURING EUKARYOTIC DNA REPLICATION
    Stephen Bell; Fiscal Year: 2000
    ....
  15. MCM PROTEIN FUNCTION DURING EUKARYOTIC DNA REPLICATION
    Stephen Bell; Fiscal Year: 2001
    ....
  16. INITIATION OF DNA REPLICATION OF YEAST CHROMOSOMES
    Stephen Bell; Fiscal Year: 2001
    ....