Daniel G Anderson


Affiliation: Massachusetts Institute of Technology
Country: USA


  1. Luo X, Wang W, Dorkin J, Veiseh O, Chang P, Abutbul Ionita I, et al. Poly(glycoamidoamine) brush nanomaterials for systemic siRNA delivery in vivo. Biomater Sci. 2016;5:38-40 pubmed publisher
    ..GluN4C10 polymer brush nanoparticles, a lead material, demonstrated significantly improved delivery efficiency for siRNA against factor VII (FVII) in mice compared to poly(glycoamidoamine) brush nanomaterials reported previously. ..
  2. Xue W, Dahlman J, Tammela T, Khan O, Sood S, Dave A, et al. Small RNA combination therapy for lung cancer. Proc Natl Acad Sci U S A. 2014;111:E3553-61 pubmed publisher
    ..These findings demonstrate that RNA combination therapy is possible in an autochthonous model of lung cancer and provide preclinical support for the use of small RNA therapies in patients who have cancer. ..
  3. Wesselhoeft R, Kowalski P, Parker Hale F, Huang Y, Bisaria N, Anderson D. RNA Circularization Diminishes Immunogenicity and Can Extend Translation Duration In Vivo. Mol Cell. 2019;: pubmed publisher
  4. Kowalski P, Rudra A, Miao L, Anderson D. Delivering the Messenger: Advances in Technologies for Therapeutic mRNA Delivery. Mol Ther. 2019;27:710-728 pubmed publisher
  5. Spanoudaki V, Doloff J, Huang W, Norcross S, Farah S, Langer R, et al. Simultaneous spatiotemporal tracking and oxygen sensing of transient implants in vivo using hot-spot MRI and machine learning. Proc Natl Acad Sci U S A. 2019;116:4861-4870 pubmed publisher
  6. Yin H, Xue W, Anderson D. CRISPR-Cas: a tool for cancer research and therapeutics. Nat Rev Clin Oncol. 2019;16:281-295 pubmed publisher
    ..In this Review, we discuss these applications as well as new potential uses of CRISPR to assist in cancer detection or the development of anticancer therapies. ..
  7. Eltoukhy A, Chen D, Veiseh O, Pelet J, Yin H, Dong Y, et al. Nucleic acid-mediated intracellular protein delivery by lipid-like nanoparticles. Biomaterials. 2014;35:6454-61 pubmed publisher
  8. Peng W, Dunton C, Holtz D, Parva M, Stampler K, Forwood M, et al. DNA nanotherapy for pre-neoplastic cervical lesions. Gynecol Oncol. 2013;128:101-106 pubmed publisher
    ..Topical application of a Lutrol F127 thermal gel/nanoparticle mix is illustrative of how to restrict exposure of cells to therapeutic nanoparticles, thereby allowing for targeted DNA delivery to cervical pre-cancerous lesions. ..
  9. Hong C, Eltoukhy A, Lee H, Langer R, Anderson D, Nam Y. Dendrimeric siRNA for Efficient Gene Silencing. Angew Chem Int Ed Engl. 2015;54:6740-4 pubmed publisher
    ..Condensation and gene silencing efficiencies increase with the increased generation of siRNA dendrimers due to a high charge density and structural flexibility. ..

More Information


  1. Wesselhoeft R, Kowalski P, Anderson D. Engineering circular RNA for potent and stable translation in eukaryotic cells. Nat Commun. 2018;9:2629 pubmed publisher
    ..This study pioneers the use of exogenous circRNA for robust and stable protein expression in eukaryotic cells and demonstrates that circRNA is a promising alternative to linear mRNA. ..
  2. Li Y, Park A, Mou H, Colpan C, Bizhanova A, Akama Garren E, et al. A versatile reporter system for CRISPR-mediated chromosomal rearrangements. Genome Biol. 2015;16:111 pubmed publisher
    ..Moreover, we detect Cas9 cleavage at the fourth nucleotide on the non-complementary strand, leading to staggered instead of blunt DNA breaks. These reporters allow mechanisms of chromosomal rearrangements to be investigated. ..
  3. Kanasty R, Vegas A, Ceo L, Maier M, Charisse K, Nair J, et al. Sequence-Defined Oligomers from Hydroxyproline Building Blocks for Parallel Synthesis Applications. Angew Chem Int Ed Engl. 2016;55:9529-33 pubmed publisher
    ..We then adapted this method to the parallel synthesis of hundreds of materials in 96-well plates. This strategy provides a platform for the screening of libraries of modified biomolecules. ..
  4. Kauffman K, Mir F, Jhunjhunwala S, Kaczmarek J, Hurtado J, Yang J, et al. Efficacy and immunogenicity of unmodified and pseudouridine-modified mRNA delivered systemically with lipid nanoparticles in vivo. Biomaterials. 2016;109:78-87 pubmed publisher
    ..This study provides insight into the immune responses to mRNA lipid nanoparticles, and suggests that pseudouridine modifications may be unnecessary for therapeutic application of mRNA in the liver. ..
  5. Kaczmarek J, Patel A, Kauffman K, Fenton O, Webber M, Heartlein M, et al. Polymer-Lipid Nanoparticles for Systemic Delivery of mRNA to the Lungs. Angew Chem Int Ed Engl. 2016;55:13808-13812 pubmed publisher
    ..To our knowledge, this is the first report of the systemic administration of mRNA for delivery to the lungs using degradable polymer-lipid nanoparticles. ..
  6. Kauffman K, Webber M, Anderson D. Materials for non-viral intracellular delivery of messenger RNA therapeutics. J Control Release. 2016;240:227-234 pubmed publisher
    ..In so doing, it is the intention of this review to provide a comprehensive look at the progress and possibilities in applying nucleic acid delivery technology specifically toward the emerging area of mRNA therapeutics. ..
  7. Kauffman K, Oberli M, Dorkin J, Hurtado J, Kaczmarek J, Bhadani S, et al. Rapid, Single-Cell Analysis and Discovery of Vectored mRNA Transfection In Vivo with a loxP-Flanked tdTomato Reporter Mouse. Mol Ther Nucleic Acids. 2018;10:55-63 pubmed publisher
    ..We believe that the system described here may facilitate the identification and characterization of mRNA delivery vectors to new tissues and cell types. ..
  8. Yin H, Bogorad R, Barnes C, Walsh S, Zhuang I, Nonaka H, et al. RNAi-nanoparticulate manipulation of gene expression as a new functional genomics tool in the liver. J Hepatol. 2016;64:899-907 pubmed publisher
    ..Our data show that siRNA-nanoparticulate manipulation of gene expression can provide the reversible control of organ size in adult animals, which presents a new avenue for the investigation of complex regulatory networks in liver. ..
  9. Jhunjhunwala S, Alvarez D, Aresta DaSilva S, Tang K, Tang B, Greiner D, et al. Frontline Science: Splenic progenitors aid in maintaining high neutrophil numbers at sites of sterile chronic inflammation. J Leukoc Biol. 2016;100:253-60 pubmed publisher
    ..Our results provide new insight into the role for splenic neutrophils and their progenitors and establish a model to study neutrophil function during sterile inflammation. ..
  10. Chahal J, Khan O, Cooper C, McPartlan J, Tsosie J, Tilley L, et al. Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose. Proc Natl Acad Sci U S A. 2016;113:E4133-42 pubmed publisher
    ..The ability to generate viable, contaminant-free vaccines within days, to single or multiple antigens, may have broad utility for a range of diseases. ..
  11. Ragelle H, Naba A, Larson B, Zhou F, Prijić M, Whittaker C, et al. Comprehensive proteomic characterization of stem cell-derived extracellular matrices. Biomaterials. 2017;128:147-159 pubmed publisher
  12. Kaczmarek J, Kowalski P, Anderson D. Advances in the delivery of RNA therapeutics: from concept to clinical reality. Genome Med. 2017;9:60 pubmed publisher