Adam Amsterdam

Summary

Affiliation: Massachusetts Institute of Technology
Country: USA

Publications

  1. pmc Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development
    Christine Insinna
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 0509, USA
    Neural Dev 5:12. 2010
  2. ncbi request reprint Mutagenesis strategies in zebrafish for identifying genes involved in development and disease
    Adam Amsterdam
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue E17 340, Cambridge, MA 02139, USA
    Trends Genet 22:473-8. 2006
  3. ncbi request reprint Transgenes as screening tools to probe and manipulate the zebrafish genome
    Adam Amsterdam
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA
    Dev Dyn 234:255-68. 2005
  4. ncbi request reprint Insertional mutagenesis in zebrafish
    Adam Amsterdam
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Dev Dyn 228:523-34. 2003
  5. ncbi request reprint Insertional mutagenesis in zebrafish: genes for development, genes for disease
    Adam Amsterdam
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue E17 340, Cambridge, MA 02139, USA
    Brief Funct Genomic Proteomic 5:19-23. 2006
  6. pmc A zebrafish screen for craniofacial mutants identifies wdr68 as a highly conserved gene required for endothelin-1 expression
    Robert M Nissen
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    BMC Dev Biol 6:28. 2006
  7. pmc DTL/CDT2 is essential for both CDT1 regulation and the early G2/M checkpoint
    Christopher L Sansam
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 20:3117-29. 2006
  8. pmc Loss of p53 synthesis in zebrafish tumors with ribosomal protein gene mutations
    Alyson W MacInnes
    The David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139
    Proc Natl Acad Sci U S A 105:10408-13. 2008
  9. ncbi request reprint A genetic screen in zebrafish identifies the mutants vps18, nf2 and foie gras as models of liver disease
    Kirsten C Sadler
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge MA 02139, USA
    Development 132:3561-72. 2005
  10. pmc Zebrafish Hagoromo mutants up-regulate fgf8 postembryonically and develop neuroblastoma
    Adam Amsterdam
    David H Koch Institute of Integrative Cancer Research, Cambridge, MA 02139, USA
    Mol Cancer Res 7:841-50. 2009

Collaborators

Detail Information

Publications29

  1. pmc Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development
    Christine Insinna
    Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 0509, USA
    Neural Dev 5:12. 2010
    ..Proper morphogenesis of photoreceptor neurons is essential for their normal function and survival. We have characterized a zebrafish mutation, cannonball, that completely disrupts photoreceptor morphogenesis...
  2. ncbi request reprint Mutagenesis strategies in zebrafish for identifying genes involved in development and disease
    Adam Amsterdam
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue E17 340, Cambridge, MA 02139, USA
    Trends Genet 22:473-8. 2006
    ..We then review how both forward genetic screens and reverse genetic techniques, such as morpholinos and TILLING, and transgenics are being used to develop models of human disease...
  3. ncbi request reprint Transgenes as screening tools to probe and manipulate the zebrafish genome
    Adam Amsterdam
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA
    Dev Dyn 234:255-68. 2005
    ..We review here some of the recent developments in the emerging field of zebrafish developmental genomics and transgenesis...
  4. ncbi request reprint Insertional mutagenesis in zebrafish
    Adam Amsterdam
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Dev Dyn 228:523-34. 2003
    ..Here, I will review the methodology of this approach and the results to date, as well as other potential ways to use insertional mutagenesis for genetic screens...
  5. ncbi request reprint Insertional mutagenesis in zebrafish: genes for development, genes for disease
    Adam Amsterdam
    Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue E17 340, Cambridge, MA 02139, USA
    Brief Funct Genomic Proteomic 5:19-23. 2006
    ..Furthermore, while all of these mutants are embryonic lethal in their homozygous state, we are investigating the heterozygous adults for additional phenotypes, such as cancer predisposition...
  6. pmc A zebrafish screen for craniofacial mutants identifies wdr68 as a highly conserved gene required for endothelin-1 expression
    Robert M Nissen
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    BMC Dev Biol 6:28. 2006
    ..However, many essential genes required for craniofacial development remain to be identified...
  7. pmc DTL/CDT2 is essential for both CDT1 regulation and the early G2/M checkpoint
    Christopher L Sansam
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 20:3117-29. 2006
    ..First, it is an essential component of the CUL4-DDB1 complex that controls CDT1 levels, thereby preventing rereplication. Second, it is required for the early G2/M checkpoint...
  8. pmc Loss of p53 synthesis in zebrafish tumors with ribosomal protein gene mutations
    Alyson W MacInnes
    The David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139
    Proc Natl Acad Sci U S A 105:10408-13. 2008
    ..Our results show that the appropriate amount of numerous ribosomal proteins is required for p53 protein production in vivo and that disruption of this regulation most likely contributes to tumorigenesis...
  9. ncbi request reprint A genetic screen in zebrafish identifies the mutants vps18, nf2 and foie gras as models of liver disease
    Kirsten C Sadler
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge MA 02139, USA
    Development 132:3561-72. 2005
    ..These mutants illustrate the utility of zebrafish as a model for studying liver development and disease, and provide valuable tools for investigating the molecular pathogenesis of congenital biliary disorders and fatty liver disease...
  10. pmc Zebrafish Hagoromo mutants up-regulate fgf8 postembryonically and develop neuroblastoma
    Adam Amsterdam
    David H Koch Institute of Integrative Cancer Research, Cambridge, MA 02139, USA
    Mol Cancer Res 7:841-50. 2009
    ..Although fgf8 overexpression is known to be associated with breast and prostate cancer in mammals, this study provides the first evidence that fgf8 misregulation can lead to neural tumors...
  11. pmc A vertebrate gene, ticrr, is an essential checkpoint and replication regulator
    Christopher L Sansam
    David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Genes Dev 24:183-94. 2010
    ..It remains to be determined whether Ticrr represents the vertebrate ortholog of the yeast pre-IC component Sld3, or a hitherto unknown metazoan replication and checkpoint regulator...
  12. pmc Many ribosomal protein mutations are associated with growth impairment and tumor predisposition in zebrafish
    Kevin Lai
    Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
    Dev Dyn 238:76-85. 2009
    ....
  13. pmc Highly aneuploid zebrafish malignant peripheral nerve sheath tumors have genetic alterations similar to human cancers
    Guangjun Zhang
    Department of Biology and David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 107:16940-5. 2010
    ..Our results suggest that the zebrafish is a useful model in which to study aneuploidy in human cancer and in which to identify candidate genes that may act as drivers in fish and potentially also in human tumors...
  14. ncbi request reprint Combined haploid and insertional mutation screen in the zebrafish
    Elizabeth Wiellette
    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA
    Genesis 40:231-40. 2004
    ....
  15. ncbi request reprint Retroviral-mediated insertional mutagenesis in zebrafish
    Adam Amsterdam
    Center for Cancer Research and Department of Biology, Massachusetts Institute for Technology, Cambridge, Massachusetts 01239, USA
    Methods Cell Biol 77:3-20. 2004
  16. pmc Identification of 315 genes essential for early zebrafish development
    Adam Amsterdam
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 101:12792-7. 2004
    ..Thus, the trait of being a genetically essential gene is conserved in evolution. This mutant collection should be a valuable resource for diverse studies of cell and developmental biology...
  17. ncbi request reprint Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development
    Gregory Golling
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
    Nat Genet 31:135-40. 2002
    ....
  18. pmc High-throughput selection of retrovirus producer cell lines leads to markedly improved efficiency of germ line-transmissible insertions in zebra fish
    Wenbiao Chen
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    J Virol 76:2192-8. 2002
    ..We discuss potential applications of this highly efficient method for generating germ line-transmissible insertions in a vertebrate..
  19. pmc Retroviral-mediated Insertional Mutagenesis in Zebrafish
    Adam Amsterdam
    Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, Massachusetts, USA
    Methods Cell Biol 104:59-82. 2011
    ..The major complementary approaches and new uses of this technique include:..
  20. ncbi request reprint Zebrafish foxi one modulates cellular responses to Fgf signaling required for the integrity of ear and jaw patterning
    Robert M Nissen
    Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    Development 130:2543-54. 2003
    ..We propose that correct placement of survival/proliferation cues is essential for shaping the pharyngeal cartilages and that evolutionary links between jaw and ear formation can be traced to Fgf-Foxi1-Pax8 pathways...
  21. ncbi request reprint TAZ, a transcriptional modulator of mesenchymal stem cell differentiation
    Jeong Ho Hong
    Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
    Science 309:1074-8. 2005
    ..These results indicate that TAZ functions as a molecular rheostat that modulates MSC differentiation...
  22. ncbi request reprint A genetic screen in zebrafish identifies cilia genes as a principal cause of cystic kidney
    Zhaoxia Sun
    MIT, Center for Cancer Research and Biology Department, Building E17 Room 340, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Development 131:4085-93. 2004
    ..Our results also suggest that lesions in genes involved in cilia formation and function are the predominant cause of cystic kidney disease, and that the genes identified here are excellent candidates for novel human PKD genes...
  23. pmc Many ribosomal protein genes are cancer genes in zebrafish
    Adam Amsterdam
    Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
    PLoS Biol 2:E139. 2004
    ..Our findings suggest that many RP genes may act as haploinsufficient tumor suppressors in fish. Many RP genes might also be cancer genes in humans, where their role in tumorigenesis could easily have escaped detection up to now...
  24. pmc Identification of zebrafish insertional mutants with defects in visual system development and function
    Jeffrey M Gross
    Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Genetics 170:245-61. 2005
    ..The affected genes in these mutants highlight a diverse set of proteins necessary for the development, maintenance, and function of the vertebrate visual system...
  25. pmc The identification of zebrafish mutants showing alterations in senescence-associated biomarkers
    Shuji Kishi
    Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Genet 4:e1000152. 2008
    ..Our current strategy of mutant screening for a senescence-associated biomarker in zebrafish embryos may thus prove to be a useful new tool for the genetic dissection of vertebrate stress response and senescence mechanisms...
  26. pmc Insertional mutagenesis strategies in zebrafish
    Sridhar Sivasubbu
    Institute of Genomics and Integrative Biology, Council for Scientific and Industrial Research, Mall Road, Delhi 110007, India
    Genome Biol 8:S9. 2007
    ....
  27. ncbi request reprint Inactivation of serine protease Matriptase1a by its inhibitor Hai1 is required for epithelial integrity of the zebrafish epidermis
    Thomas J Carney
    Max Planck Institute of Immunobiology, Stuebeweg 51, D 79108 Freiburg, Germany
    Development 134:3461-71. 2007
    ..Our work provides direct genetic evidence for antagonistic in vivo roles of Hai1 and Matriptase1a to regulate skin homeostasis and remodeling...
  28. ncbi request reprint A zebrafish sox9 gene required for cartilage morphogenesis
    Yi Lin Yan
    Institute of Neuroscience, University of Oregon, Eugene 97403, USA
    Development 129:5065-79. 2002
    ..Likewise, calcein labeling revealed that early bone formation was largely unaffected in jef (sox9a) mutants. These studies show that jef (sox9a) is essential for both morphogenesis of condensations and overt cartilage differentiation...
  29. ncbi request reprint Uses of GFP in transgenic vertebrates
    Sean Megason
    Beckman Institute of Biological Imaging, California Institute of Technology, Pasadena, CA 91125, USA
    Methods Biochem Anal 47:285-303. 2006