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Genomes and Genes | Adam AmsterdamSummaryAffiliation: Massachusetts Institute of Technology Country: USA Publications
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Publications
Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor developmentChristine Insinna
Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226 0509, USA
Neural Dev 5:12. 2010..Proper morphogenesis of photoreceptor neurons is essential for their normal function and survival. We have characterized a zebrafish mutation, cannonball, that completely disrupts photoreceptor morphogenesis...
Mutagenesis strategies in zebrafish for identifying genes involved in development and diseaseAdam Amsterdam
Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue E17 340, Cambridge, MA 02139, USA
Trends Genet 22:473-8. 2006..We then review how both forward genetic screens and reverse genetic techniques, such as morpholinos and TILLING, and transgenics are being used to develop models of human disease...- Transgenes as screening tools to probe and manipulate the zebrafish genomeAdam Amsterdam
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA
Dev Dyn 234:255-68. 2005..We review here some of the recent developments in the emerging field of zebrafish developmental genomics and transgenesis... - Insertional mutagenesis in zebrafishAdam Amsterdam
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Dev Dyn 228:523-34. 2003..Here, I will review the methodology of this approach and the results to date, as well as other potential ways to use insertional mutagenesis for genetic screens... - Insertional mutagenesis in zebrafish: genes for development, genes for diseaseAdam Amsterdam
Center for Cancer Research, Massachusetts Institute of Technology, 77 Massachusetts Avenue E17 340, Cambridge, MA 02139, USA
Brief Funct Genomic Proteomic 5:19-23. 2006..Furthermore, while all of these mutants are embryonic lethal in their homozygous state, we are investigating the heterozygous adults for additional phenotypes, such as cancer predisposition... - A zebrafish screen for craniofacial mutants identifies wdr68 as a highly conserved gene required for endothelin-1 expressionRobert M Nissen
Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
BMC Dev Biol 6:28. 2006..However, many essential genes required for craniofacial development remain to be identified... - DTL/CDT2 is essential for both CDT1 regulation and the early G2/M checkpointChristopher L Sansam
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Genes Dev 20:3117-29. 2006..First, it is an essential component of the CUL4-DDB1 complex that controls CDT1 levels, thereby preventing rereplication. Second, it is required for the early G2/M checkpoint... - Loss of p53 synthesis in zebrafish tumors with ribosomal protein gene mutationsAlyson W MacInnes
The David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139
Proc Natl Acad Sci U S A 105:10408-13. 2008..Our results show that the appropriate amount of numerous ribosomal proteins is required for p53 protein production in vivo and that disruption of this regulation most likely contributes to tumorigenesis... - A genetic screen in zebrafish identifies the mutants vps18, nf2 and foie gras as models of liver diseaseKirsten C Sadler
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge MA 02139, USA
Development 132:3561-72. 2005..These mutants illustrate the utility of zebrafish as a model for studying liver development and disease, and provide valuable tools for investigating the molecular pathogenesis of congenital biliary disorders and fatty liver disease... - Zebrafish Hagoromo mutants up-regulate fgf8 postembryonically and develop neuroblastomaAdam Amsterdam
David H Koch Institute of Integrative Cancer Research, Cambridge, MA 02139, USA
Mol Cancer Res 7:841-50. 2009..Although fgf8 overexpression is known to be associated with breast and prostate cancer in mammals, this study provides the first evidence that fgf8 misregulation can lead to neural tumors... - A vertebrate gene, ticrr, is an essential checkpoint and replication regulatorChristopher L Sansam
David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Genes Dev 24:183-94. 2010..It remains to be determined whether Ticrr represents the vertebrate ortholog of the yeast pre-IC component Sld3, or a hitherto unknown metazoan replication and checkpoint regulator... - Many ribosomal protein mutations are associated with growth impairment and tumor predisposition in zebrafishKevin Lai
Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA
Dev Dyn 238:76-85. 2009.... - Highly aneuploid zebrafish malignant peripheral nerve sheath tumors have genetic alterations similar to human cancersGuangjun Zhang
Department of Biology and David H Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 107:16940-5. 2010..Our results suggest that the zebrafish is a useful model in which to study aneuploidy in human cancer and in which to identify candidate genes that may act as drivers in fish and potentially also in human tumors... - Combined haploid and insertional mutation screen in the zebrafishElizabeth Wiellette
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA
Genesis 40:231-40. 2004.... - Retroviral-mediated insertional mutagenesis in zebrafishAdam Amsterdam
Center for Cancer Research and Department of Biology, Massachusetts Institute for Technology, Cambridge, Massachusetts 01239, USA
Methods Cell Biol 77:3-20. 2004 - Identification of 315 genes essential for early zebrafish developmentAdam Amsterdam
Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Proc Natl Acad Sci U S A 101:12792-7. 2004..Thus, the trait of being a genetically essential gene is conserved in evolution. This mutant collection should be a valuable resource for diverse studies of cell and developmental biology... - Insertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate developmentGregory Golling
Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA
Nat Genet 31:135-40. 2002.... - High-throughput selection of retrovirus producer cell lines leads to markedly improved efficiency of germ line-transmissible insertions in zebra fishWenbiao Chen
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
J Virol 76:2192-8. 2002..We discuss potential applications of this highly efficient method for generating germ line-transmissible insertions in a vertebrate.. - Retroviral-mediated Insertional Mutagenesis in ZebrafishAdam Amsterdam
Koch Institute for Integrative Cancer Research, Massachusetts Institute for Technology, Cambridge, Massachusetts, USA
Methods Cell Biol 104:59-82. 2011..The major complementary approaches and new uses of this technique include:.. - Zebrafish foxi one modulates cellular responses to Fgf signaling required for the integrity of ear and jaw patterningRobert M Nissen
Center for Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
Development 130:2543-54. 2003..We propose that correct placement of survival/proliferation cues is essential for shaping the pharyngeal cartilages and that evolutionary links between jaw and ear formation can be traced to Fgf-Foxi1-Pax8 pathways... - TAZ, a transcriptional modulator of mesenchymal stem cell differentiationJeong Ho Hong
Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, E18 580, Cambridge, MA 02139, USA
Science 309:1074-8. 2005..These results indicate that TAZ functions as a molecular rheostat that modulates MSC differentiation... - A genetic screen in zebrafish identifies cilia genes as a principal cause of cystic kidneyZhaoxia Sun
MIT, Center for Cancer Research and Biology Department, Building E17 Room 340, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
Development 131:4085-93. 2004..Our results also suggest that lesions in genes involved in cilia formation and function are the predominant cause of cystic kidney disease, and that the genes identified here are excellent candidates for novel human PKD genes... - Many ribosomal protein genes are cancer genes in zebrafishAdam Amsterdam
Center for Cancer Research, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA
PLoS Biol 2:E139. 2004..Our findings suggest that many RP genes may act as haploinsufficient tumor suppressors in fish. Many RP genes might also be cancer genes in humans, where their role in tumorigenesis could easily have escaped detection up to now... - Identification of zebrafish insertional mutants with defects in visual system development and functionJeffrey M Gross
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA
Genetics 170:245-61. 2005..The affected genes in these mutants highlight a diverse set of proteins necessary for the development, maintenance, and function of the vertebrate visual system... - The identification of zebrafish mutants showing alterations in senescence-associated biomarkersShuji Kishi
Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts, United States of America
PLoS Genet 4:e1000152. 2008..Our current strategy of mutant screening for a senescence-associated biomarker in zebrafish embryos may thus prove to be a useful new tool for the genetic dissection of vertebrate stress response and senescence mechanisms... - Insertional mutagenesis strategies in zebrafishSridhar Sivasubbu
Institute of Genomics and Integrative Biology, Council for Scientific and Industrial Research, Mall Road, Delhi 110007, India
Genome Biol 8:S9. 2007.... - Inactivation of serine protease Matriptase1a by its inhibitor Hai1 is required for epithelial integrity of the zebrafish epidermisThomas J Carney
Max Planck Institute of Immunobiology, Stuebeweg 51, D 79108 Freiburg, Germany
Development 134:3461-71. 2007..Our work provides direct genetic evidence for antagonistic in vivo roles of Hai1 and Matriptase1a to regulate skin homeostasis and remodeling... - A zebrafish sox9 gene required for cartilage morphogenesisYi Lin Yan
Institute of Neuroscience, University of Oregon, Eugene 97403, USA
Development 129:5065-79. 2002..Likewise, calcein labeling revealed that early bone formation was largely unaffected in jef (sox9a) mutants. These studies show that jef (sox9a) is essential for both morphogenesis of condensations and overt cartilage differentiation... - Uses of GFP in transgenic vertebratesSean Megason
Beckman Institute of Biological Imaging, California Institute of Technology, Pasadena, CA 91125, USA
Methods Biochem Anal 47:285-303. 2006