Marcus Altfeld

Summary

Affiliation: Massachusetts General Hospital
Country: USA

Publications

  1. pmc Temporal effect of HLA-B*57 on viral control during primary HIV-1 infection
    Sagar A Vaidya
    Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital, Boston, MA, USA
    Retrovirology 10:139. 2013
  2. pmc Human immunodeficiency virus type 1 specific cytotoxic T lymphocyte responses in Chinese infected with HIV-1 B'/C Recombinant (CRF07_BC)
    Jianping Chen
    State Key Laboratory for Infectious Disease Control and Prevention, National Center for AIDS STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China
    Retrovirology 4:62. 2007
  3. pmc Standardization of cytokine flow cytometry assays
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:13. 2005
  4. pmc DCs and NK cells: critical effectors in the immune response to HIV-1
    Marcus Altfeld
    Ragon Institute of MGH, MIT and Harvard, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Nat Rev Immunol 11:176-86. 2011
  5. ncbi request reprint Hitting HIV where it hurts: an alternative approach to HIV vaccine design
    Marcus Altfeld
    Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, MA 02129, USA
    Trends Immunol 27:504-10. 2006
  6. pmc The majority of currently circulating human immunodeficiency virus type 1 clade B viruses fail to prime cytotoxic T-lymphocyte responses against an otherwise immunodominant HLA-A2-restricted epitope: implications for vaccine design
    Marcus Altfeld
    Partners AIDS Research Center, Massachusetts General Hospital, 149 13th St, Boston, MA 02129, USA
    J Virol 79:5000-5. 2005
  7. ncbi request reprint T(H)1 to T(H)2 shift of cytokines in peripheral blood of HIV-infected patients is detectable by reverse transcriptase polymerase chain reaction but not by enzyme-linked immunosorbent assay under nonstimulated conditions
    M Altfeld
    Department of Internal Medicine I, University of Bonn, Germany
    J Acquir Immune Defic Syndr 23:287-94. 2000
  8. ncbi request reprint The role of CD4(+) T helper cells in the cytotoxic T lymphocyte response to HIV-1
    M Altfeld
    Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02114, USA
    Curr Opin Immunol 12:375-80. 2000
  9. pmc Selective escape from CD8+ T-cell responses represents a major driving force of human immunodeficiency virus type 1 (HIV-1) sequence diversity and reveals constraints on HIV-1 evolution
    Todd M Allen
    Howard Hughes Medical Institute, Partners AIDS Research Center, and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA
    J Virol 79:13239-49. 2005
  10. ncbi request reprint Control of human immunodeficiency virus replication by cytotoxic T lymphocytes targeting subdominant epitopes
    Nicole Frahm
    Partners AIDS Research Center, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Immunol 7:173-8. 2006

Research Grants

  1. CD8+ T cell function in HIV-1 infection
    Marcus Altfeld; Fiscal Year: 2010
  2. NK Cell Function in HIV-1 Infection
    Marcus Altfeld; Fiscal Year: 2010
  3. CD8+ T cell function in HIV-1 infection
    Marcus Altfeld; Fiscal Year: 2007
  4. NK Cell Function in HIV-1 Infection
    Marcus Altfeld; Fiscal Year: 2007
  5. CTL Responses to Regulatory and Accessory HIV-1 Proteins
    Marcus Altfeld; Fiscal Year: 2006
  6. TLR-mediated gender differences in HIV-1 pathogenesis
    Marcus Altfeld; Fiscal Year: 2010
  7. Modulation of HIV-1 specific T cell function by TLR ligands
    Marcus Altfeld; Fiscal Year: 2007

Detail Information

Publications109 found, 100 shown here

  1. pmc Temporal effect of HLA-B*57 on viral control during primary HIV-1 infection
    Sagar A Vaidya
    Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital, Boston, MA, USA
    Retrovirology 10:139. 2013
    ..This study sought to determine the role of HLA-B alleles in viral control during the acute phase of HIV-1 infection and establishment of the early viral load set point (VLSP)...
  2. pmc Human immunodeficiency virus type 1 specific cytotoxic T lymphocyte responses in Chinese infected with HIV-1 B'/C Recombinant (CRF07_BC)
    Jianping Chen
    State Key Laboratory for Infectious Disease Control and Prevention, National Center for AIDS STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China
    Retrovirology 4:62. 2007
    ....
  3. pmc Standardization of cytokine flow cytometry assays
    Holden T Maecker
    BD Biosciences, San Jose, USA
    BMC Immunol 6:13. 2005
    ....
  4. pmc DCs and NK cells: critical effectors in the immune response to HIV-1
    Marcus Altfeld
    Ragon Institute of MGH, MIT and Harvard, Massachusetts General Hospital, Charlestown, Massachusetts 02129, USA
    Nat Rev Immunol 11:176-86. 2011
    ..The importance of understanding DC and NK cell crosstalk during HIV infection for the development of effective antiviral strategies is also discussed...
  5. ncbi request reprint Hitting HIV where it hurts: an alternative approach to HIV vaccine design
    Marcus Altfeld
    Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, MA 02129, USA
    Trends Immunol 27:504-10. 2006
    ....
  6. pmc The majority of currently circulating human immunodeficiency virus type 1 clade B viruses fail to prime cytotoxic T-lymphocyte responses against an otherwise immunodominant HLA-A2-restricted epitope: implications for vaccine design
    Marcus Altfeld
    Partners AIDS Research Center, Massachusetts General Hospital, 149 13th St, Boston, MA 02129, USA
    J Virol 79:5000-5. 2005
    ....
  7. ncbi request reprint T(H)1 to T(H)2 shift of cytokines in peripheral blood of HIV-infected patients is detectable by reverse transcriptase polymerase chain reaction but not by enzyme-linked immunosorbent assay under nonstimulated conditions
    M Altfeld
    Department of Internal Medicine I, University of Bonn, Germany
    J Acquir Immune Defic Syndr 23:287-94. 2000
    ..Therefore, we determined tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), IL-4, IL-10, and interferon-gamma (IFN-gamma) mRNA and serum levels in HIV-infected patients under nonstimulated conditions...
  8. ncbi request reprint The role of CD4(+) T helper cells in the cytotoxic T lymphocyte response to HIV-1
    M Altfeld
    Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02114, USA
    Curr Opin Immunol 12:375-80. 2000
    ....
  9. pmc Selective escape from CD8+ T-cell responses represents a major driving force of human immunodeficiency virus type 1 (HIV-1) sequence diversity and reveals constraints on HIV-1 evolution
    Todd M Allen
    Howard Hughes Medical Institute, Partners AIDS Research Center, and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA
    J Virol 79:13239-49. 2005
    ..The stereotypic nature of acquired mutations provides support for biochemical constraints limiting HIV-1 evolution and for the impact of CD8 escape mutations on viral fitness...
  10. ncbi request reprint Control of human immunodeficiency virus replication by cytotoxic T lymphocytes targeting subdominant epitopes
    Nicole Frahm
    Partners AIDS Research Center, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts 02115, USA
    Nat Immunol 7:173-8. 2006
    ..These data suggest that subdominant responses can contribute to in vivo viral control and that high HLA allele frequencies may drive the elimination of subdominant yet effective epitopes from circulating viral populations...
  11. pmc Mutually exclusive T-cell receptor induction and differential susceptibility to human immunodeficiency virus type 1 mutational escape associated with a two-amino-acid difference between HLA class I subtypes
    Xu G Yu
    Partners AIDS Research Center, Massachusetts General Hospital, 149 13th Street, Room 5212, Charlestown, MA 02129, USA
    J Virol 81:1619-31. 2007
    ..These data show the influence of HLA allele subtypes on TCR selection and indicate that extensive TCR diversity is not a prerequisite to prevention of allowable viral mutations...
  12. pmc Selection, transmission, and reversion of an antigen-processing cytotoxic T-lymphocyte escape mutation in human immunodeficiency virus type 1 infection
    Todd M Allen
    Howard Hughes Medical Institute, Partners AIDS Research Center, Infectious Disease Division, Massachusetts General Hospital, and Division of AIDS, Harvard Medical School, Boston, MA, USA
    J Virol 78:7069-78. 2004
    ..Moreover, reversion of these mutations in the absence of the original immune pressure reveals the potential plasticity of immunologically selected evolutionary changes...
  13. pmc The major genetic determinants of HIV-1 control affect HLA class I peptide presentation
    Florencia Pereyra
    Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology MIT and Harvard, Boston, MA, USA
    Science 330:1551-7. 2010
    ..These results implicate the nature of the HLA-viral peptide interaction as the major factor modulating durable control of HIV infection...
  14. ncbi request reprint Single-stranded RNA derived from HIV-1 serves as a potent activator of NK cells
    Galit Alter
    Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, MA 02129, USA
    J Immunol 178:7658-66. 2007
    ....
  15. pmc Effects of thymic selection of the T-cell repertoire on HLA class I-associated control of HIV infection
    Andrej Kosmrlj
    Ragon Institute of MGH, MIT and Harvard, Boston, Massachusetts 02114, USA
    Nature 465:350-4. 2010
    ..Our results provide a conceptual framework that unifies diverse empirical observations, and have implications for vaccination strategies...
  16. pmc Immune-driven recombination and loss of control after HIV superinfection
    Hendrik Streeck
    Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    J Exp Med 205:1789-96. 2008
    ..These data also support a role for cellular immune pressures in driving the selection of new recombinant forms of HIV...
  17. pmc Antigen load and viral sequence diversification determine the functional profile of HIV-1-specific CD8+ T cells
    Hendrik Streeck
    Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, United States of America
    PLoS Med 5:e100. 2008
    ....
  18. pmc Upregulation of PD-L1 on monocytes and dendritic cells by HIV-1 derived TLR ligands
    Angela Meier
    Partners AIDS Research Center, Massachusetts General Hospital, Division of AIDS, Harvard Medical School, Boston, Massachusetts, USA
    AIDS 22:655-8. 2008
    ..These data suggest a mechanism through which HIV-1-derived TLR ligands might contribute to the functional impairment of virus-specific PD-1-positive T cells by inducing the upregulation of PD-L1 on antigen-presenting cells...
  19. pmc Loss of HIV-1-specific CD8+ T cell proliferation after acute HIV-1 infection and restoration by vaccine-induced HIV-1-specific CD4+ T cells
    Mathias Lichterfeld
    Partners AIDS Research Center, Massachusetts General Hospital, 149 13th St, Boston, MA 02129, USA
    J Exp Med 200:701-12. 2004
    ..This identification of a reversible defect in cell-mediated immunity in chronic HIV-1 infection has important implications for immunotherapeutic interventions...
  20. pmc Ligand-independent exhaustion of killer immunoglobulin-like receptor-positive CD8+ T cells in human immunodeficiency virus type 1 infection
    Galit Alter
    Partners AIDS Research Center, Massachusetts General Hospital, 149 13th Street, Boston, MA 02129, USA
    J Virol 82:9668-77. 2008
    ....
  21. pmc De novo generation of escape variant-specific CD8+ T-cell responses following cytotoxic T-lymphocyte escape in chronic human immunodeficiency virus type 1 infection
    Todd M Allen
    Partners AIDS Research Center, Massachusetts General Hospital, Boston, 02129, USA
    J Virol 79:12952-60. 2005
    ....
  22. pmc Selective depletion of high-avidity human immunodeficiency virus type 1 (HIV-1)-specific CD8+ T cells after early HIV-1 infection
    Mathias Lichterfeld
    Partners AIDS Research Center, Massachusetts General Hospital, and Division of AIDS, Harvard Medical School, Boston, MA 02129, USA
    J Virol 81:4199-214. 2007
    ..These data suggest that high-avidity HIV-1-specific CD8+ T-cell clones are recruited during early infection but are subsequently lost in the presence of persistent high-level viral replication...
  23. pmc Differential regulation of toll-like receptor pathways in acute and chronic HIV-1 infection
    J Judy Chang
    Ragon Institute of Massachusetts General Hospital, Massachusetts Institute Technology and Harvard, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, USA
    AIDS 26:533-41. 2012
    ..Studies assessing the consequences of HIV-1 infection on the ability of innate immune cells to respond to TLR stimulation have come to varying conclusions...
  24. pmc MyD88-dependent immune activation mediated by human immunodeficiency virus type 1-encoded Toll-like receptor ligands
    Angela Meier
    Partners AIDS Research Center, Massachusetts General Hospital, 149 13th Street, Boston, MA 02129, USA
    J Virol 81:8180-91. 2007
    ..These data provide an initial rationale for inhibiting the TLR pathway to directly reduce the chronic immune activation induced by HIV-1 and the associated immune pathogenesis...
  25. ncbi request reprint Influence of HLA-B57 on clinical presentation and viral control during acute HIV-1 infection
    Marcus Altfeld
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Boston, Massachusetts, USA
    AIDS 17:2581-91. 2003
    ..However, their impact on viral replication during acute HIV-1 infection is not known...
  26. ncbi request reprint Immunological and virological impact of highly active antiretroviral therapy initiated during acute HIV-1 infection
    Hendrik Streeck
    Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital, Charlestown, MA, 02129, USA
    J Infect Dis 194:734-9. 2006
    ..However, despite these immunological changes, no differences in viremia or in the CD4+ T cell count were found 6 months after HAART was stopped, when treated subjects were compared with untreated subjects...
  27. pmc Limited durability of viral control following treated acute HIV infection
    Daniel E Kaufmann
    Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    PLoS Med 1:e36. 2004
    ..However, the durability of such control remains unclear. Here we present longitudinal follow-up of a single-arm, open-label study assessing the impact of STI in the setting of acute HIV-1 infection...
  28. pmc MHC class I chain-related protein A shedding in chronic HIV-1 infection is associated with profound NK cell dysfunction
    Anne Nolting
    Ragon Institute of Massachusetts General Hospital, Harvard University, Boston, MA, USA
    Virology 406:12-20. 2010
    ..Thus, like tumors, HIV-1 may indirectly suppress NK cell recognition of HIV-1-infected CD4+ T cells by enhancing NKG2D-ligand secretion into the serum resulting in a profound impairment of NK cell function...
  29. pmc Human immunodeficiency virus type 1-specific CD8+ T-cell responses during primary infection are major determinants of the viral set point and loss of CD4+ T cells
    Hendrik Streeck
    Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA
    J Virol 83:7641-8. 2009
    ..Taken together, these data show that the specificity of the initial CTL response to HIV is critical for the subsequent control of viremia and have important implications for the rational selection of antigens for future HIV-1 vaccines...
  30. ncbi request reprint Sequential deregulation of NK cell subset distribution and function starting in acute HIV-1 infection
    Galit Alter
    Partners AIDS Research Center, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, USA
    Blood 106:3366-9. 2005
    ..Taken together, these studies demonstrate a sequential impairment of NK cell function with persistent viral replication resulting from a progressive deregulation of NK cell subsets with distinct functional properties...
  31. pmc Comprehensive analysis of human immunodeficiency virus type 1-specific CD4 responses reveals marked immunodominance of gag and nef and the presence of broadly recognized peptides
    Daniel E Kaufmann
    Partners AIDS Research Center and Infectious Disease Unit, Howard Hughes Medical Institute, Massachusetts General Hospital, and Division of AIDS, Boston, Massachusetts 02114, USA
    J Virol 78:4463-77. 2004
    ..These findings have implications for the design of immunotherapeutic strategies and for testing candidate HIV vaccines...
  32. ncbi request reprint T cell receptor cross-recognition of an HIV-1 CD8+ T cell epitope presented by closely related alleles from the HLA-A3 superfamily
    Mathias Lichterfeld
    Partners AIDS Research Center, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, MA, USA
    Int Immunol 18:1179-88. 2006
    ..Thus, despite distinct TCR recognition of HLA-A3 or HLA-A11 presented HIV-1 peptides in the vast majority of cases, specific TCRs can cross-recognize their antigen in the context of both HLA-A3 and HLA-A11...
  33. ncbi request reprint Evolution of innate and adaptive effector cell functions during acute HIV-1 infection
    Galit Alter
    Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital, Charlestown, MA 02129, USA
    J Infect Dis 195:1452-60. 2007
    ....
  34. pmc Immune selection for altered antigen processing leads to cytotoxic T lymphocyte escape in chronic HIV-1 infection
    Rika Draenert
    Howard Hughes Medical Institute, Charlestown, MA 02129, USA
    J Exp Med 199:905-15. 2004
    ..These results demonstrate that allele-associated sequence variation within the flanking region of CTL epitopes can alter antigen processing. Identifying such mutations is of major relevance in the construction of vaccine sequences...
  35. pmc Recognition of a defined region within p24 gag by CD8+ T cells during primary human immunodeficiency virus type 1 infection in individuals expressing protective HLA class I alleles
    Hendrik Streeck
    Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA
    J Virol 81:7725-31. 2007
    ....
  36. pmc Marked epitope- and allele-specific differences in rates of mutation in human immunodeficiency type 1 (HIV-1) Gag, Pol, and Nef cytotoxic T-lymphocyte epitopes in acute/early HIV-1 infection
    Zabrina L Brumme
    Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Virol 82:9216-27. 2008
    ..Dramatic differences in escape and reversion rates across codons, genes, and HLA restrictions are observed, highlighting the complexity of viral adaptation to the host immune response...
  37. ncbi request reprint HIV-1 Nef is preferentially recognized by CD8 T cells in primary HIV-1 infection despite a relatively high degree of genetic diversity
    Mathias Lichterfeld
    Partners AIDS Research Center and Howard Hughes Medical Institute, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts 02129, USA
    AIDS 18:1383-92. 2004
    ....
  38. ncbi request reprint HIV-1 specific CD8+ T cells with an effector phenotype and control of viral replication
    Christoph Hess
    Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy and Immunology, Harvard Medical School, Massachusetts, USA
    Lancet 363:863-6. 2004
    ..Our data show that full maturation of virus-specific CD8+ T cells is possible in the context of HIV-1-infection, and suggest that such maturation might be important in viral control...
  39. pmc Enhanced detection of human immunodeficiency virus type 1-specific T-cell responses to highly variable regions by using peptides based on autologous virus sequences
    Marcus Altfeld
    Partners AIDS Research Center and Infectious Disease Unit, Massachusetts General Hospital, and Division of AIDS, Harvard Medical School, Boston, Massachusetts, USA
    J Virol 77:7330-40. 2003
    ..They also demonstrate that CD8 T-cell responses to the variable HIV-1 proteins are more common than previously reported...
  40. pmc A viral CTL escape mutation leading to immunoglobulin-like transcript 4-mediated functional inhibition of myelomonocytic cells
    Mathias Lichterfeld
    Partners AIDS Research Center, Massachusetts General Hospital, and Harvard University Center for AIDS Research, Boston, MA 02129, USA
    J Exp Med 204:2813-24. 2007
    ....
  41. ncbi request reprint HIV-1 superinfection despite broad CD8+ T-cell responses containing replication of the primary virus
    Marcus Altfeld
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts 02129, USA
    Nature 420:434-9. 2002
    ..The lack of cross-protective immunity for closely related HIV-1 strains, despite persistent recognition of multiple CD8 epitopes, has important implications for public health and vaccine development...
  42. pmc Epidemiologically linked transmission of HIV-1 illustrates the impact of host genetics on virological outcome
    Hendrik Streeck
    Partners AIDS Research Center, Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, Charlestown, Massachusetts 02129, USA
    AIDS 23:259-62. 2009
    ..These data suggest that genetic factors play an important role in the outcome of HIV-1 infection despite early therapy...
  43. pmc HLA-Cw*0102-restricted HIV-1 p24 epitope variants can modulate the binding of the inhibitory KIR2DL2 receptor and primary NK cell function
    Lena Fadda
    Ragon Institute of MGH, MIT and Harvard, Charlestown, Massachusetts, United States of America
    PLoS Pathog 8:e1002805. 2012
    ....
  44. pmc Preserved function of regulatory T cells in chronic HIV-1 infection despite decreased numbers in blood and tissue
    Mathieu Angin
    Ragon Institute of MGH, MIT and Harvard, Charlestown, MA 02129 2000, USA
    J Infect Dis 205:1495-500. 2012
    ..Despite quantitative changes in Tregs, HIV-1 infection was not associated with an impairment of ex vivo suppressive function of flow-sorted Tregs in both HIV controllers and untreated chronic progressors...
  45. pmc Induction of strong HIV-1-specific CD4+ T-cell responses using an HIV-1 gp120/NefTat vaccine adjuvanted with AS02A in antiretroviral-treated HIV-1-infected individuals
    Mathias Lichterfeld
    Ragon Institute of Massachusetts General Hospital, Boston, USA
    J Acquir Immune Defic Syndr 59:1-9. 2012
    ..Induction of HIV-1-specific CD4(+) T-cell responses by therapeutic vaccination represents an attractive intervention to potentially increase immune control of HIV-1...
  46. ncbi request reprint High degree of inter-clade cross-reactivity of HIV-1-specific T cell responses at the single peptide level
    Xu G Yu
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, 149 13th Street, Boston, MA 02129, USA
    AIDS 19:1449-56. 2005
    ....
  47. ncbi request reprint HIV-1 viral escape in infancy followed by emergence of a variant-specific CTL response
    Margaret E Feeney
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    J Immunol 174:7524-30. 2005
    ..Taken together, these findings indicate that the developing immune system of children may exhibit greater plasticity in responding to a continually evolving chronic viral infection...
  48. pmc Epithelial adhesion molecules can inhibit HIV-1-specific CD8⁺ T-cell functions
    Hendrik Streeck
    Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    Blood 117:5112-22. 2011
    ....
  49. ncbi request reprint Low perforin and elevated SHIP-1 expression is associated with functional anergy of natural killer cells in chronic HIV-1 infection
    Galit Alter
    Partners AIDS Research Center, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts, USA
    AIDS 20:1549-51. 2006
    ..These CD3-CD56-CD16+ NK cells expressed SHIP-1 and had significantly reduced perforin levels. This observation suggests a mechanism for the reduced functional activity of CD3-CD56-CD16+ NK cells in chronic HIV-1 infection...
  50. ncbi request reprint Comparison of overlapping peptide sets for detection of antiviral CD8 and CD4 T cell responses
    Rika Draenert
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, MA 02129, USA
    J Immunol Methods 275:19-29. 2003
    ..We conclude that peptides ranging from 15 to 20 amino acids yield similar results in IFN-gamma-based Elispot and ICS assays, and that all are likely to underestimate the true breadth of responses to a given reference strain of virus...
  51. pmc HIV-1 adaptation to NK-cell-mediated immune pressure
    Galit Alter
    Ragon Institute at MGH, MIT and Harvard, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02129, USA
    Nature 476:96-100. 2011
    ..NK cells might therefore have a previously underappreciated role in contributing to viral evolution...
  52. pmc Protective HLA class I alleles that restrict acute-phase CD8+ T-cell responses are associated with viral escape mutations located in highly conserved regions of human immunodeficiency virus type 1
    Yaoyu E Wang
    Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, USA
    J Virol 83:1845-55. 2009
    ....
  53. ncbi request reprint HIV-1 Vpu represents a minor target for cytotoxic T lymphocytes in HIV-1-infection
    Marylyn M Addo
    Partners AIDS Research Center and Infectious Disease Unit, Massachusetts General Hospital, Boston, MA 02114, USA
    AIDS 16:1071-3. 2002
    ..The epitope was shown to be highly conserved among HIV clade B sequences and is restricted by HLA-A*3303, an HLA allele commonly seen in Asian and west-African populations...
  54. doi request reprint Early viral replication in lymph nodes provides HIV with a means by which to escape NK-cell-mediated control
    Rutger Luteijn
    Ragon Institute of MGH, MIT and Harvard, Boston, MA 02129, USA
    Eur J Immunol 41:2729-40. 2011
    ....
  55. pmc Common HIV-1 peptide variants mediate differential binding of KIR3DL1 to HLA-Bw4 molecules
    Lena Fadda
    Ragon Institute of MGH, MIT and Harvard, Massachusetts General Hospital, Bldg 149, 13th Street, Charlestown, MA 02129, USA
    J Virol 85:5970-4. 2011
    ....
  56. ncbi request reprint HIV-1-specific cytotoxicity is preferentially mediated by a subset of CD8(+) T cells producing both interferon-gamma and tumor necrosis factor-alpha
    Mathias Lichterfeld
    Partners AIDS Research Center, Massachusetts General Hospital, 149 13th St, Rm 6613, Boston, MA 02129, USA
    Blood 104:487-94. 2004
    ..These results suggest that HIV-1-specific cytotoxicity of CD8(+) T cells is preferentially mediated by a subset of CD8(+) T cells secreting both interferon-gamma and TNF-alpha...
  57. ncbi request reprint Increased natural killer cell activity in viremic HIV-1 infection
    Galit Alter
    Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Boston 02129, USA
    J Immunol 173:5305-11. 2004
    ..Taken together, these data demonstrate that viremic HIV-1 infection is associated with a reduction in NK cell numbers and a perturbation of NK cell subsets, but increased overall NK cell activity...
  58. pmc Differential natural killer cell-mediated inhibition of HIV-1 replication based on distinct KIR/HLA subtypes
    Galit Alter
    Partners AIDS Research Center and Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, MA 02129, USA
    J Exp Med 204:3027-36. 2007
    ..These data provide the first functional evidence that variation at the KIR locus influences the effectiveness of NK cell activity in the containment of viral replication...
  59. pmc Reduced frequencies of NKp30+NKp46+, CD161+, and NKG2D+ NK cells in acute HCV infection may predict viral clearance
    Galit Alter
    Ragon Institute of MGH, MIT and Harvard, Boston, MA, USA
    J Hepatol 55:278-88. 2011
    ..Epidemiologic data support a role for particular NK cell receptor bearing populations in this control, yet the mechanism by which NK cells respond to HCV early in infection is unknown...
  60. pmc Dysregulated Tim-3 expression on natural killer cells is associated with increased Galectin-9 levels in HIV-1 infection
    Stephanie Jost
    Ragon Institute of MGH, MIT and Harvard, 400 Technology Square, Cambridge, MA 02139, USA
    Retrovirology 10:74. 2013
    ..Whether HIV-1 infection modulates the expression of Tim-3 on NK cells, or the levels of its ligand Galectin-9 (Gal-9), and how signaling through these molecules affects the NK cell response to HIV-1 remains inadequately understood...
  61. pmc Alterations in natural killer cell receptor profiles during HIV type 1 disease progression among chronically infected South African adults
    Ambrose H W Wong
    Ragon Institute of MGH, MIT, and Harvard formerly Partners AIDS Research Center, Massachusetts General Hospital, Charleston, Massachusetts 02129, USA
    AIDS Res Hum Retroviruses 26:459-69. 2010
    ..These findings suggest that modulation of receptor expression on NK cells may play a role in HIV-1 pathogenesis, and provide new insights on immunological changes in advanced HIV-1 disease...
  62. pmc Higher expression of several interferon-stimulated genes in HIV-1-infected females after adjusting for the level of viral replication
    J Judy Chang
    Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard, Harvard Medical School, Cambridge, MA 02139, USA
    J Infect Dis 208:830-8. 2013
    ....
  63. pmc HLA-B63 presents HLA-B57/B58-restricted cytotoxic T-lymphocyte epitopes and is associated with low human immunodeficiency virus load
    Nicole Frahm
    Partners AIDS Research Center, Massachusetts General Hospital, No 5214, 149 13th Street, Charlestown, MA 02129, USA
    J Virol 79:10218-25. 2005
    ..The data support an important role of the presented epitope in mediating relative control of HIV replication and help to better define immune correlates of controlled HIV infection...
  64. ncbi request reprint Differences in the expressed HLA class I alleles effect the differential clustering of HIV type 1-specific T cell responses in infected Chinese and caucasians
    Xu G Yu
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts 02129, USA
    AIDS Res Hum Retroviruses 20:557-64. 2004
    ....
  65. pmc Consistent patterns in the development and immunodominance of human immunodeficiency virus type 1 (HIV-1)-specific CD8+ T-cell responses following acute HIV-1 infection
    Xu G Yu
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA
    J Virol 76:8690-701. 2002
    ..These studies have important implications for vaccine design and evaluation...
  66. pmc HLA class I subtype-dependent expansion of KIR3DS1+ and KIR3DL1+ NK cells during acute human immunodeficiency virus type 1 infection
    Galit Alter
    Ragon Institute of MGH, MIT, Boston, Massachusetts, USA
    J Virol 83:6798-805. 2009
    ..These data demonstrate for the first time the HLA class I subtype-dependent expansion of specific KIR(+) NK cells during an acute viral infection in humans...
  67. pmc Coordinate linkage of HIV evolution reveals regions of immunological vulnerability
    Vincent Dahirel
    Ragon Institute of Massachusetts General Hospital, and Harvard University, Boston, MA 02129, USA
    Proc Natl Acad Sci U S A 108:11530-5. 2011
    ..Targeting such regions with higher order evolutionary constraints provides a novel approach to immunogen design for a vaccine against HIV and other rapidly mutating viruses...
  68. pmc Rapid loss of dendritic cell and monocyte responses to TLR ligands following venipuncture
    Angela Meier
    Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    J Immunol Methods 339:132-40. 2008
    ..Overnight shipment or extended rest of whole blood before processing therefore severely compromises the ability of APCs to respond to TLR ligands, and this has to be taken into consideration when designing multicenter trials...
  69. ncbi request reprint Immunodominance of HIV-1-specific CD8(+) T-cell responses in acute HIV-1 infection: at the crossroads of viral and host genetics
    Mathias Lichterfeld
    Partners AIDS Research Center, Massachusetts General Hospital, Boston, MA 02129, USA
    Trends Immunol 26:166-71. 2005
    ....
  70. ncbi request reprint CD107a as a functional marker for the identification of natural killer cell activity
    Galit Alter
    Partners AIDS Research Center, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Massachusetts, USA
    J Immunol Methods 294:15-22. 2004
    ..Cumulatively, the data presented here demonstrate that CD107a is a sensitive marker of NK cell activity...
  71. ncbi request reprint IL-8 responsiveness defines a subset of CD8 T cells poised to kill
    Christoph Hess
    Center for Immunology and Inflammatory Diseases, Division of Rheumatology, Allergy and Immunology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    Blood 104:3463-71. 2004
    ..Thus, CXCR1 identifies a subset of CD8 T cells poised for immediate cytotoxicity and early recruitment into sites of innate immune system activation...
  72. pmc IL-10 induces aberrant deletion of dendritic cells by natural killer cells in the context of HIV infection
    Galit Alter
    Ragon Institute of MGH, MIT, and Harvard, Charlestown, Massachusetts 02129, USA
    J Clin Invest 120:1905-13. 2010
    ....
  73. pmc Expansion of pre-existing, lymph node-localized CD8+ T cells during supervised treatment interruptions in chronic HIV-1 infection
    Marcus Altfeld
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, MA 02129, USA
    J Clin Invest 109:837-43. 2002
    ..Furthermore, the enhanced CD8+ T cell responses observed during STI in chronically infected individuals is largely due to expansion of pre-existing virus-specific CD8+ T cells, rather than the induction of novel responses...
  74. pmc Sex differences in the Toll-like receptor-mediated response of plasmacytoid dendritic cells to HIV-1
    Angela Meier
    Ragon Institute of Massachusetts General Hospital, Massachusetts Institute of Technology and Harvard University, Boston, Massachusetts, USA
    Nat Med 15:955-9. 2009
    ..Modulation of the TLR7 pathway in pDCs may therefore represent a new approach to reduce HIV-1-associated pathology...
  75. doi request reprint Rapid ex vivo isolation and long-term culture of human Th17 cells
    Hendrik Streeck
    Partners AIDS Research Center, Division of Infectious Diseases, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02129, United States
    J Immunol Methods 333:115-25. 2008
    ..In conclusion, this technique will allow for the first time the direct, ex vivo analysis of phenotypic and functional properties of Th17 cells...
  76. ncbi request reprint Loss of HIV-1-specific T cell proliferation in chronic HIV-1 infection: cause or consequence of viral replication?
    Mathias Lichterfeld
    Partners AIDS Research Center, Massachusetts General Hospital, Boston, Massachusetts 02129, USA
    AIDS 19:1225-7. 2005
  77. ncbi request reprint Impact of intrapeptide epitope location on CD8 T cell recognition: implications for design of overlapping peptide panels
    Rika Draenert
    Howard Hughes Medical Institute and Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School Division of AIDS, Boston, MA, USA
    AIDS 18:871-6. 2004
    ..The effect of epitope location within such peptides is not known but may influence recognition...
  78. pmc Functionally inert HIV-specific cytotoxic T lymphocytes do not play a major role in chronically infected adults and children
    P J Goulder
    Partners AIDS Research Center, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts 02129, USA
    J Exp Med 192:1819-32. 2000
    ..These data together show that functionally inert CTLs do not play a significant role in chronic pediatric or adult HIV infection...
  79. pmc Crippling HIV one mutation at a time
    Todd M Allen
    Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA
    J Exp Med 205:1003-7. 2008
    ..These data suggest that targeting these and other conserved viral regions may be the key to developing an effective HIV-1 vaccine...
  80. ncbi request reprint Important contribution of p15 Gag-specific responses to the total Gag-specific CTL responses
    Xu G Yu
    Partners AIDS Research Center, Infectious Disease Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA
    AIDS 16:321-8. 2002
    ..However, the extent to which p15 Gag is targeted by the host immune system in natural infection as well as precise cytotoxic T lymphocyte (CTL) epitopes within this protein remains to be defined...
  81. pmc Rapid reversion of sequence polymorphisms dominates early human immunodeficiency virus type 1 evolution
    Bin Li
    Partners AIDS Research Center, MGH East, CNY 6616, 149 13th Street, Charlestown, MA 02129, USA
    J Virol 81:193-201. 2007
    ....
  82. pmc HIV-1-specific interleukin-21+ CD4+ T cell responses contribute to durable viral control through the modulation of HIV-specific CD8+ T cell function
    Mathieu F Chevalier
    Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital, and Harvard Medical School, Boston, MA 02129, USA
    J Virol 85:733-41. 2011
    ..These data suggest that HIV-1-specific IL-21(+) CD4(+) T cell responses might contribute to the control of viral replication in humans and are likely to be of great importance for vaccine design...
  83. pmc Cellular immune responses and viral diversity in individuals treated during acute and early HIV-1 infection
    M Altfeld
    Partners AIDS Research Center and Infectious Disease Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts 02129, USA
    J Exp Med 193:169-80. 2001
    ..These data also provide rationale for therapeutic immunization aimed at broadening CTL responses in treated primary HIV infection...
  84. ncbi request reprint NK cell function in HIV-1 infection
    Galit Alter
    Partners AIDS Research Center, Infectious Disease Unit, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, MA 02129, USA
    Curr Mol Med 6:621-9. 2006
    ..Here we present a review of the literature pertaining to both the effect of HIV-1 infection on NK cell activity and the potential role that this subset of cells may play in controlling HIV-1 disease...
  85. ncbi request reprint HIV-1 superinfection
    Todd M Allen
    Partners AIDS Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA
    J Allergy Clin Immunol 112:829-35; quiz 836. 2003
    ..Herein we review the published reports of HIV-1 superinfection and highlight studies providing additional insight into the potential for HIV-1 superinfections to affect the global epidemic...
  86. pmc Innate immune activation in primary HIV-1 infection
    J Judy Chang
    Ragon Institute of MGH, MIT, and Harvard, Massachusetts General Hospital, and Harvard Medical School, Boston, MA 02129, USA
    J Infect Dis 202:S297-301. 2010
    ..Understanding the complexity of how innate responses affect the outcome of HIV-1 infection will help in the development of vaccines that can use innate immunity to enhance viral control with minimal pathogenesis...
  87. ncbi request reprint Parallel decrease in neurotoxin quinolinic acid and soluble tumor necrosis factor receptor p75 in serum during highly active antiretroviral therapy of HIV type 1 disease
    M P Look
    Department of General Internal Medicine, University of Bonn, Germany
    AIDS Res Hum Retroviruses 16:1215-21. 2000
    ..This eventually led to a decrease in quinolinic acid formation. The parallel reduction of the immune activation marker sTNFR p75 supports this hypothesis...
  88. ncbi request reprint Cytotoxic T-lymphocyte (CTL) responses directed against regulatory and accessory proteins in HIV-1 infection
    M M Addo
    Partners AIDS Research Center, Massachusetts General Hospital Harvard Medical School, Boston, Massachusetts, USA
    DNA Cell Biol 21:671-8. 2002
    ....
  89. pmc The HIV-1 regulatory proteins Tat and Rev are frequently targeted by cytotoxic T lymphocytes derived from HIV-1-infected individuals
    M M Addo
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA
    Proc Natl Acad Sci U S A 98:1781-6. 2001
    ..Despite the small size of these proteins, multiple CTL epitopes were identified in each. These data indicate that Tat and Rev are frequently targeted by CTL in natural HIV-1 infection and may be important targets for HIV vaccines...
  90. ncbi request reprint Evolution and transmission of stable CTL escape mutations in HIV infection
    P J Goulder
    Partners AIDS Research Center, Massachusetts General Hospital and Division of AIDS, Harvard Medical School, Boston, Massachusetts 02114, USA
    Nature 412:334-8. 2001
    ..These data suggest that CTL escape mutations in epitopes associated with suppression of viraemia will accumulate as the epidemic progresses, and therefore have important implications for vaccine design...
  91. ncbi request reprint Vpr is preferentially targeted by CTL during HIV-1 infection
    M Altfeld
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02129, USA
    J Immunol 167:2743-52. 2001
    ..These findings will be important in evaluating the specificity and breadth of immune responses during acute and chronic infection, and in the design and testing of candidate HIV vaccines...
  92. pmc NK cells in HIV-1 infection: evidence for their role in the control of HIV-1 infection
    G Alter
    Partners AIDS Research Center, Massachusetts General Hospital, Charlestown, MA 02129, USA
    J Intern Med 265:29-42. 2009
    ..In this review we highlight recent evidence that suggests that NK cells influence the clinical fate of HIV-infected individuals...
  93. ncbi request reprint Regulatory and accessory HIV-1 proteins: potential targets for HIV-1 vaccines?
    X G Yu
    Partners AIDS Research Center and Infectious Disease Division, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA
    Curr Med Chem 12:741-7. 2005
    ..A multi-component vaccine, with the inclusion of these proteins plus structural proteins remains the most promising choice for an effective AIDS vaccine...
  94. pmc Comprehensive epitope analysis of human immunodeficiency virus type 1 (HIV-1)-specific T-cell responses directed against the entire expressed HIV-1 genome demonstrate broadly directed responses, but no correlation to viral load
    M M Addo
    Partners AIDS Research Center, Massachusetts General Hospital and Harvard Medical School Fenway Community Health Center Lemuel Shattuck Hospital, Boston, Massachusetts 02129, USA
    J Virol 77:2081-92. 2003
    ....
  95. pmc Limited value of PCR for detection of Toxoplasma gondii in blood from human immunodeficiency virus-infected patients
    C Franzen
    Department of Internal Medicine I, University of Cologne, Germany
    J Clin Microbiol 35:2639-41. 1997
    ..The sensitivity was only 25%, but the specificity was very high (100%), so this technique may be useful for discriminating between cerebral toxoplasmosis and other brain diseases which may be mistaken for toxoplasmosis...
  96. ncbi request reprint Rev activity determines sensitivity of HIV-1-infected primary T cells to CTL killing
    Kevin R Bobbitt
    Department of Internal Medicine, The University of Michigan, Ann Arbor, MI 48109, USA
    Immunity 18:289-99. 2003
    ..Thus, rev alleles with low activity may have a selective advantage in infected people with effective immune responses...
  97. ncbi request reprint Viremia control despite escape from a rapid and potent autologous neutralizing antibody response after therapy cessation in an HIV-1-infected individual
    David C Montefiori
    Department of Surgery, Duke University Medical Center, Durham, NC 27710, USA
    J Immunol 170:3906-14. 2003
    ..We postulate that an early effective neutralizing Ab response can provide long-term clinical benefits despite neutralization escape...
  98. pmc Clustering patterns of cytotoxic T-lymphocyte epitopes in human immunodeficiency virus type 1 (HIV-1) proteins reveal imprints of immune evasion on HIV-1 global variation
    Karina Yusim
    Los Alamos National Laboratory, Los Alamos, New Mexico 87545 Santa Fe Institute, Santa Fe, New Mexico 87501, USA
    J Virol 76:8757-68. 2002
    ....
  99. ncbi request reprint Dominant influence of HLA-B in mediating the potential co-evolution of HIV and HLA
    Photini Kiepiela
    HIV Pathogenesis Programme, The Doris Duke Medical Research Institute, University of KwaZuluNatal, 719 Umbilo Road, Durban 4015, South Africa
    Nature 432:769-75. 2004
    ..The dominant involvement of HLA-B in influencing HIV disease outcome is of specific relevance to the direction of HIV research and to vaccine design...
  100. ncbi request reprint Differential immunogenicity of HIV-1 clade C proteins in eliciting CD8+ and CD4+ cell responses
    Danni Ramduth
    HIV Pathogenesis Programme, Doris Duke Medical Research Institute, University of KwaZulu Natal, Durban, South Africa
    J Infect Dis 192:1588-96. 2005
    ..The relative immunogenicity of human immunodeficiency virus type 1 (HIV-1) proteins for CD8+ and CD4+ cell responses has not been defined...
  101. ncbi request reprint AIDS restriction HLA allotypes target distinct intervals of HIV-1 pathogenesis
    Xiaojiang Gao
    Laboratory of Genomic Diversity, NCI Frederick, Basic Research Program, SAIC Frederick, P O Box B, Frederick, Maryland 21702, USA
    Nat Med 11:1290-2. 2005
    ..The discrete timing of HLA allele influence suggests alternative functional mechanisms in immune defense against this dynamic and chronic immunosuppressive disease...

Research Grants18

  1. CD8+ T cell function in HIV-1 infection
    Marcus Altfeld; Fiscal Year: 2010
    ....
  2. NK Cell Function in HIV-1 Infection
    Marcus Altfeld; Fiscal Year: 2010
    ..Impact of the KIR/HLA compound genotype on NK cell function b. Impact of the peptides presented by HLA on KIR-mediated recognition by NK cells ..
  3. CD8+ T cell function in HIV-1 infection
    Marcus Altfeld; Fiscal Year: 2007
    ....
  4. NK Cell Function in HIV-1 Infection
    Marcus Altfeld; Fiscal Year: 2007
    ..Analysis of the mechanisms that determine NK cell recognition of HIV-1 infected cells a. Impact of the KIR/HLA compound genotype on NK cell function b. Impact of the peptides presented by HLA on KIR-mediated recognition by NK cells. ..
  5. CTL Responses to Regulatory and Accessory HIV-1 Proteins
    Marcus Altfeld; Fiscal Year: 2006
    ....
  6. TLR-mediated gender differences in HIV-1 pathogenesis
    Marcus Altfeld; Fiscal Year: 2010
    ....
  7. Modulation of HIV-1 specific T cell function by TLR ligands
    Marcus Altfeld; Fiscal Year: 2007
    ....