SANJAY SHUKLA

Summary

Affiliation: Marshfield Clinic
Country: USA

Publications

  1. pmc Shift in Staphylococcus aureus clone linked to an infected tattoo
    Mary E Stemper
    Marshfield Laboratories, Marshfield, Wisconsin 54449, USA
    Emerg Infect Dis 12:1444-6. 2006
  2. pmc Comparative whole-genome mapping to determine Staphylococcus aureus genome size, virulence motifs, and clonality
    Sanjay K Shukla
    Marshfield Clinic Research Foundation, Marshfield, Wisconsin, USA
    J Clin Microbiol 50:3526-33. 2012
  3. pmc Complete genome sequence and lifestyle of black-pigmented Corynebacterium aurimucosum ATCC 700975 (formerly C. nigricans CN-1) isolated from a vaginal swab of a woman with spontaneous abortion
    Eva Trost
    Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universitat Bielefeld, Universitätsstrasse 27, D 33615 Bielefeld, Germany
    BMC Genomics 11:91. 2010
  4. pmc Optical mapping reveals a large genetic inversion between two methicillin-resistant Staphylococcus aureus strains
    Sanjay K Shukla
    Marshfield Clinic Research Foundation, 1000 North Oak Avenue, Marshfield, Wisconsin 54449, USA
    J Bacteriol 191:5717-23. 2009
  5. pmc Contribution of polymorphisms in ankA, gltA, and groESL in defining genetic variants of Anaplasma phagocytophilum
    Sanjay K Shukla
    Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, 1000 North Oak Avenue, Marshfield, WI 54449, USA
    J Clin Microbiol 45:2312-5. 2007
  6. pmc Virulence genes and genotypic associations in nasal carriage, community-associated methicillin-susceptible and methicillin-resistant USA400 Staphylococcus aureus isolates
    Sanjay K Shukla
    Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, Marshfield, WI 54449, USA
    J Clin Microbiol 48:3582-92. 2010
  7. ncbi request reprint CA-MRSA triangulation: virulent strains, susceptible hosts, and contaminated environments
    Sanjay K Shukla
    Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation WI 54449, USA
    WMJ 105:21-3. 2006
  8. pmc Community-associated methicillin-resistant Staphylococcus aureus and its emerging virulence
    Sanjay K Shukla
    Molecular Microbiology Laboratory, Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, WI 54449, USA
    Clin Med Res 3:57-60. 2005
  9. pmc Molecular characteristics of nosocomial and Native American community-associated methicillin-resistant Staphylococcus aureus clones from rural Wisconsin
    Sanjay K Shukla
    Molecular Microbiology Laboratory, Clinical Research Center, Marshfield Clinic Research Foundation, 1000 North Oak Ave, Marshfield, WI 54449, USA
    J Clin Microbiol 42:3752-7. 2004
  10. pmc Novel polymorphisms in mec genes and a new mec complex type in methicillin-resistant Staphylococcus aureus isolates obtained in rural Wisconsin
    Sanjay K Shukla
    Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449, USA
    Antimicrob Agents Chemother 48:3080-5. 2004

Research Grants

Collaborators

Detail Information

Publications36

  1. pmc Shift in Staphylococcus aureus clone linked to an infected tattoo
    Mary E Stemper
    Marshfield Laboratories, Marshfield, Wisconsin 54449, USA
    Emerg Infect Dis 12:1444-6. 2006
    ..Case timeline indicated a displacement of USA400 clone by USA300 clone. The USA300 index case was associated with an infected new tattoo...
  2. pmc Comparative whole-genome mapping to determine Staphylococcus aureus genome size, virulence motifs, and clonality
    Sanjay K Shukla
    Marshfield Clinic Research Foundation, Marshfield, Wisconsin, USA
    J Clin Microbiol 50:3526-33. 2012
    ..These results showed that WGMs could be used not only to genotype S. aureus but also to identify genetic motifs in MRSA that may predict virulence...
  3. pmc Complete genome sequence and lifestyle of black-pigmented Corynebacterium aurimucosum ATCC 700975 (formerly C. nigricans CN-1) isolated from a vaginal swab of a woman with spontaneous abortion
    Eva Trost
    Institut für Genomforschung und Systembiologie, Centrum für Biotechnologie, Universitat Bielefeld, Universitätsstrasse 27, D 33615 Bielefeld, Germany
    BMC Genomics 11:91. 2010
    ..For a better understanding of the physiology and lifestyle of this potential urogenital pathogen, the complete genome sequence of C. aurimucosum ATCC 700975 was determined...
  4. pmc Optical mapping reveals a large genetic inversion between two methicillin-resistant Staphylococcus aureus strains
    Sanjay K Shukla
    Marshfield Clinic Research Foundation, 1000 North Oak Avenue, Marshfield, Wisconsin 54449, USA
    J Bacteriol 191:5717-23. 2009
    ..At both the left and right junction sites in NRS387, an IS1181 element and a 73-bp sequence were identified as inverted repeats, which could explain the possible mechanism of the inversion event...
  5. pmc Contribution of polymorphisms in ankA, gltA, and groESL in defining genetic variants of Anaplasma phagocytophilum
    Sanjay K Shukla
    Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, 1000 North Oak Avenue, Marshfield, WI 54449, USA
    J Clin Microbiol 45:2312-5. 2007
    ..The multigene polymorphism approach holds promise for development of a genotyping scheme for this important pathogen...
  6. pmc Virulence genes and genotypic associations in nasal carriage, community-associated methicillin-susceptible and methicillin-resistant USA400 Staphylococcus aureus isolates
    Sanjay K Shukla
    Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, Marshfield, WI 54449, USA
    J Clin Microbiol 48:3582-92. 2010
    ..These results suggest that the presence of additional virulence factors in USA400 CA-MRSA strains compared to the nasal carriage and clinical MSSA strains probably contributed to their enhanced virulence...
  7. ncbi request reprint CA-MRSA triangulation: virulent strains, susceptible hosts, and contaminated environments
    Sanjay K Shukla
    Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation WI 54449, USA
    WMJ 105:21-3. 2006
  8. pmc Community-associated methicillin-resistant Staphylococcus aureus and its emerging virulence
    Sanjay K Shukla
    Molecular Microbiology Laboratory, Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, WI 54449, USA
    Clin Med Res 3:57-60. 2005
  9. pmc Molecular characteristics of nosocomial and Native American community-associated methicillin-resistant Staphylococcus aureus clones from rural Wisconsin
    Sanjay K Shukla
    Molecular Microbiology Laboratory, Clinical Research Center, Marshfield Clinic Research Foundation, 1000 North Oak Ave, Marshfield, WI 54449, USA
    J Clin Microbiol 42:3752-7. 2004
    ..The 19 mCGs were represented by diverse SCCmec types and three putative new variants referred to as SCCmec Ib, IIa, and IIb...
  10. pmc Novel polymorphisms in mec genes and a new mec complex type in methicillin-resistant Staphylococcus aureus isolates obtained in rural Wisconsin
    Sanjay K Shukla
    Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449, USA
    Antimicrob Agents Chemother 48:3080-5. 2004
    ..There was also a wide range of oxacillin MICs for strains with wild-type mecA, mecI, and mecR1 sequences, suggesting that the genetic backgrounds of clinical strains are significant in determining susceptibility to methicillin...
  11. pmc Isolation of a fastidious Bergeyella species associated with cellulitis after a cat bite and a phylogenetic comparison with Bergeyella zoohelcum strains
    Sanjay K Shukla
    Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Neillsville, Wisconsin 54449, USA
    J Clin Microbiol 42:290-3. 2004
    ..Despite sharing a close genetic relationship with other B. zoohelcum strains, this isolate was extremely fastidious in nature, raising the possibility that similar strains from cat or dog bite wound infections have been underreported...
  12. pmc Corynebacterium nigricans sp. nov.: proposed name for a black-pigmented Corynebacterium species recovered from the human female urogenital tract
    Sanjay K Shukla
    Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449, USA
    J Clin Microbiol 41:4353-8. 2003
    ..The type strain of Corynebacterium nigricans is CN-1. It is deposited in the American Type Culture Collection (assigned strain number ATCC 700975) and in the Institute Pasteur collection (assigned strain number CIP 107346)...
  13. ncbi request reprint Is a black-pigmented Corynebacterium species an opportunistic pathogen during pregnancy? Literature review and report of 3 new cases
    Sanjay K Shukla
    Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449, USA
    Clin Infect Dis 37:834-7. 2003
    ..Similarities in these cases suggest that this newly recognized Corynebacterium species might be an opportunistic pathogen in pregnancy...
  14. pmc Sporadic "transitional" community-associated methicillin-resistant Staphylococcus aureus strains from health care facilities in the United States
    Jennifer M Brady
    Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, Marshfield, WI 54449, USA
    J Clin Microbiol 45:2654-61. 2007
    ..The potential role of PVL-carrying phages and plasmids in the success of CA-MRSA clones has been discussed...
  15. pmc Emergence and spread of community-associated methicillin-resistant Staphylococcus aureus in rural Wisconsin, 1989 to 1999
    Mary E Stemper
    Clinical Research Center, Marshfield Clinic Research Foundation, 1000 North Oak Ave, Marshfield, WI 54449, USA
    J Clin Microbiol 42:5673-80. 2004
    ..Two early CA-MRSA strains (WI-33 and WI-34) in Wisconsin represent progenitors of the MW2 strain, based on their almost indistinguishable genotypic characteristics...
  16. ncbi request reprint MRSA case studies
    Kurt D Reed
    Emerging Infectious Disease Laboratory, Marshfield Clinic Research Foundation, Marshfield, WI, USA
    Methods Mol Biol 391:21-8. 2007
    ..Significant genetic diversity exists among MRSA and sensitive strains of S. aureus. It is obvious that various combinations of virulence factors contribute to disease manifestations of infected patients...
  17. ncbi request reprint An update on the epidemiology, pathogenesis and management of infective endocarditis with emphasis on Staphylococcus aureus
    Tahir Tak
    Department of Cardiology, Marshfield Clinic, 1000 N Oak Ave, Marshfield, WI 54449, USA
    WMJ 101:24-33. 2002
    ..In this review we will focus on the epidemiology, microbiology, and pathogenesis of S aureus IE, and also summarize the current guidelines for diagnosis, treatment, and prophylaxis of this clinical condition...
  18. pmc Leptotrichia amnionii sp. nov., a novel bacterium isolated from the amniotic fluid of a woman after intrauterine fetal demise
    Sanjay K Shukla
    Clinical Research Center, Marshfield Medical Research and Education Foundation, Wisconsin 54449, USA
    J Clin Microbiol 40:3346-9. 2002
    ..The 1,493-bp 16S ribosomal DNA sequence had only 96% homology with L. sanguinegens. Several phylogenetic analyses indicated that L. amnionii is a distinct species and most closely related to L. sanguiegens...
  19. pmc Staphylococcal superantigen-like genes, ssl5 and ssl8, are positively regulated by Sae and negatively by Agr in the Newman strain
    Madhulatha Pantrangi
    Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, Marshfield, WI 54449, USA
    FEMS Microbiol Lett 308:175-84. 2010
    ..The SigB mutation did not affect ssl5 and ssl8 expression, but they were downregulated in the agr/sigB double mutant, indicating that SigB probably acts synergistically with Agr in their upregulation...
  20. ncbi request reprint Second case of infective endocarditis caused by Gemella sanguinis
    Sanjay K Shukla
    Clinical Research Center, Marshfield Medical Research and Education Foundation, 1000 N Oak Ave, Marshfield, WI 54449, USA
    WMJ 101:37-9. 2002
    ..A subsequent transesophageal echocardiogram demonstrated vegetations on both mitral leaflets and severe mitral regurgitation by color flow imaging. A gram-positive coccus was isolated from the blood culture of this patient...
  21. ncbi request reprint Pulsed-field gel electrophoresis of MRSA
    Kurt D Reed
    Emerging Infectious Disease Laboratory, Marshfield Clinic Research Foundation, Marshfield, WI, USA
    Methods Mol Biol 391:59-69. 2007
    ..Banding patterns are captured by an imaging system and comparisons are made based on the Dice coefficient and the unweighted pair group method using arithmetic averages with BioNumerics software...
  22. pmc Necrotizing fasciitis due to a methicillin-sensitive Staphylococcus aureus isolate harboring an enterotoxin gene cluster
    William R Morgan
    Department of Internal Medicine, Marshfield Clinic, Marshfield, Wisconsin, USA
    J Clin Microbiol 45:668-71. 2007
    ..The etiological agent was a Staphylococcus aureus isolate harboring the enterotoxin gene cluster seg, sei, sem, sen, and seo but lacked all common toxin genes, including Panton-Valentine leukocidin...
  23. ncbi request reprint DLL3 as a candidate gene for vertebral malformations
    Philip F Giampietro
    Department of Medical Genetic Services, Marshfield Clinic, Marshfield, Wisconsin 54449, USA
    Am J Med Genet A 140:2447-53. 2006
    ....
  24. ncbi request reprint PCR analysis of nasal polyps, chronic sinusitis, and hypertrophied turbinates for DNA encoding bacterial 16S rRNA
    Gerald A Bucholtz
    Department of Allergy, Marshfield Clinic, Wisconsin 54449, USA
    Am J Rhinol 16:169-73. 2002
    ..Previously, Mycoplasma pneumoniae-specific DNA was detected in human nasal polyps using polymerase chain reaction (PCR) techniques, suggesting M. pneumoniae as a causative agent in the etiology of nasal polyps...
  25. pmc Evidence of multiple virulence subtypes in nosocomial and community-associated MRSA genotypes in companion animals from the upper midwestern and northeastern United States
    Yihan Lin
    Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, WI 54449, USA
    Clin Med Res 9:7-16. 2011
    ..We report the rate of prevalence of S. aureus and MRSA recovered from clinical samples of animals requiring treatment at veterinary clinics throughout the upper midwestern and northeastern United States...
  26. ncbi request reprint Current diagnosis and treatment of infective endocarditis
    Tahir Tak
    Department of Internal Medicine, Marshfield Clinic, 1000 North Oak Avenue, Marshfield, WI 54449, USA
    Expert Rev Anti Infect Ther 1:639-54. 2003
    ..In the case of culture-negative endocarditis or infective endocarditis caused by fastidious microorganisms, the polymerase chain reaction and probe-based diagnostic methods are available to clinical reference laboratories...
  27. pmc Birds, migration and emerging zoonoses: west nile virus, lyme disease, influenza A and enteropathogens
    Kurt D Reed
    Clinical Research Center, Marshfield Medical Research Foundation, Marshfield, Wisconsin 54449, USA
    Clin Med Res 1:5-12. 2003
    ..A better understanding of avian migration patterns and infectious diseases of birds would be useful in helping to predict future outbreaks of infections due to emerging zoonotic pathogens...
  28. pmc Rapid molecular diagnosis of lactobacillus bacteremia by terminal restriction fragment length polymorphism analysis of the 16S rRNA gene
    Jeffrey E Christensen
    Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449, USA
    Clin Med Res 2:37-45. 2004
    ..We sought to apply this technique to the identification of Lactobacillus spp. from three cases of bacteremia...
  29. ncbi request reprint Fatal brain abscess due to community-associated methicillin-resistant Staphylococcus aureus strain USA300
    Costi D Sifri
    Division of Infectious Diseases and International Health, University of Virginia Health System, Charlottesville, VA 22908 1361, USA
    Clin Infect Dis 45:e113-7. 2007
    ..Community-onset pyogenic brain abscess should be added to the growing list of life-threatening invasive infections caused by epidemic community-acquired methicillin-resistant S. aureus...
  30. pmc Molecular diagnosis of infective endocarditis: a helpful addition to the Duke criteria
    Tahir Tak
    Department of Internal Medicine, Division of Cardiology, University of North Texas Health Science Center, Fort Worth, Texas 76107, USA
    Clin Med Res 2:206-8. 2004
  31. pmc Genetic variants of Anaplasma phagocytophilum infecting dogs in Western Washington State
    Florence M Poitout
    Phoenix Central Laboratory, 11620 Airport Rd, Everett, WA 98204 3742, USA
    J Clin Microbiol 43:796-801. 2005
    ..The zoonotic importance of A. phagocytophilum should support an increase in surveillance for horses and people residing in this area...
  32. ncbi request reprint Community-associated methicillin-resistant Staphylococcus aureus: it's time to pay attention
    Sanjay K Shukla
    WMJ 105:25-7. 2006
  33. pmc Importance of primer specificity for PCR detection of Anaplasma phagocytophila among Ixodes scapularis ticks from Wisconsin
    Sanjay K Shukla
    J Clin Microbiol 41:4006. 2003
  34. pmc Analysis of 16S rRNA gene sequences and circulating cell-free DNA from plasma of chronic fatigue syndrome and non-fatigued subjects
    Suzanne D Vernon
    Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
    BMC Microbiol 2:39. 2002
    ..Plasma DNA from 34 CFS and 55 non-fatigued subjects was assessed to determine plasma DNA concentration and the presence of bacterial 16S ribosomal DNA (rDNA) sequences...
  35. ncbi request reprint Absence of Mycoplasma species DNA in chronic fatigue syndrome
    Suzanne D Vernon
    J Med Microbiol 52:1027-8. 2003
  36. ncbi request reprint Nitrincola lacisaponensis gen. nov., sp. nov., a novel alkaliphilic bacterium isolated from an alkaline, saline lake
    Pedro A Dimitriu
    Department of Biological Sciences, University of Missouri Rolla, Rolla, MO 65401, USA
    Int J Syst Evol Microbiol 55:2273-8. 2005
    ..nov., sp. nov. The type strain is 4CAT (=ATCC BAA-920T=DSM 16316T)...

Research Grants3

  1. Molecular Epidemiology of Community-Associated MRSA
    SANJAY SHUKLA; Fiscal Year: 2006
    ..We will determine the presence or absences of 40 different virulence factor genes in all 320 isolates listed in aim 2 and determine evolution of virulence factor genes in contemporary CA-MRSA. ..
  2. Molecular Epidemiology of Community-Associated MRSA
    SANJAY SHUKLA; Fiscal Year: 2007
    ..We will determine the presence or absences of 40 different virulence factor genes in all 320 isolates listed in aim 2 and determine evolution of virulence factor genes in contemporary CA-MRSA. ..