Research Topics
| K D ReedSummaryAffiliation: Marshfield Clinic Country: USA Publications
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Detail Information
Publications
Laboratory testing for Lyme disease: possibilities and practicalitiesKurt D Reed
Department of Pathology, Marshfield Clinic, and the Clinical Research Center, Marshfield Medical Research Foundation, Marshfield, Wisconsin 54449, USA
J Clin Microbiol 40:319-24. 2002
Chlamydia pneumoniae infection in a breeding colony of African clawed frogs (Xenopus tropicalis)K D Reed
Clinical Research Department, Marshfield Medical Research Foundation, WI 54449, USA
Emerg Infect Dis 6:196-9. 2000..Chlamydia pneumoniae was isolated in cell cultures from four frogs. A cutaneous infection by a chytridiomycete fungus observed in two frogs could have been a cofactor in the die-off.ous Diseases..
Prevalence of granulocytic Ehrlichia infection among white-tailed deer in WisconsinE A Belongia
Marshfield Clinic and Marshfield Medical Research Foundation, Wisconsin 54449, USA
J Clin Microbiol 35:1465-8. 1997..Granulocytic Ehrlichia organisms in deer are widely distributed within the geographic range of L. scapularis in Wisconsin. Deer may serve as useful sentinels for areas where HGE transmission to humans may occur...
Immunoserologic evidence of coinfection with Borrelia burgdorferi, Babesia microti, and human granulocytic Ehrlichia species in residents of Wisconsin and MinnesotaP D Mitchell
Microbiology Section, Marshfield Laboratories, WI 54449, USA
J Clin Microbiol 34:724-7. 1996..In certain clinical settings, laboratory testing for coinfection is indicated to ensure that appropriate antimicrobial treatment is given...
Desulfovibrio desulfuricans bacteremia in a dogS K Shukla
Molecular Microbiology Laboratory, Marshfield Medical Research Foundation, Marshfield, WI 54449, USA
J Clin Microbiol 38:1701-2. 2000..Desulfovibrio desulfuricans was isolated from the blood of a dog presenting with fever, anorexia, and rear limb stiffness. The isolate was identified by 16S rRNA gene amplification and sequencing...
Isolation and characterization of a black-pigmented Corynebacterium sp. from a woman with spontaneous abortionS K Shukla
Department of Clinical Research, Marshfield Medical Research Foundation, 1000 North Oak Ave, Marshfield, Wisconsin 54449, USA
J Clin Microbiol 39:1109-13. 2001..Our strain is designated CN-1 (ATCC 700975)...
Clinical and epidemiological features of early Lyme disease and human granulocytic ehrlichiosis in WisconsinE A Belongia
Epidemiology Research Center, Marshfield Medical Research Foundation, Marshfield, WI 54449, USA
Clin Infect Dis 29:1472-7. 1999..The clinical spectrum of HGE overlaps that of Lyme disease, and physicians in areas of endemicity should consider both diseases in treating patients with a compatible rash or febrile illness...
Birds, migration and emerging zoonoses: west nile virus, lyme disease, influenza A and enteropathogensKurt D Reed
Clinical Research Center, Marshfield Medical Research Foundation, Marshfield, Wisconsin 54449, USA
Clin Med Res 1:5-12. 2003..A better understanding of avian migration patterns and infectious diseases of birds would be useful in helping to predict future outbreaks of infections due to emerging zoonotic pathogens...
Mosquito surveillance for West Nile virus in southeastern Wisconsin--2002Jennifer K Meece
Center for Tropical Disease Research and Training, University of Notre Dame, Notre Dame, Indiana, USA
Clin Med Res 1:37-42. 2003..This information coupled with infection rate data can help guide public health policies related to vector control, and may help reduce the impact on human, veterinary and bird mortality...
Polybrominated diphenyl ethers (PBDEs): new pollutants-old diseasesMuhammad Akmal Siddiqi
Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449, USA
Clin Med Res 1:281-90. 2003..Further studies are imperative in a multitude of health and environmental disciplines to determine the adverse effects and mode of action of this widespread emerging pollutant on human health...
Monkeypox, Marshfield Clinic and the Internet: leveraging information technology for public healthKurt D Reed
Department of Pathology, Marshfield Clinic, Marshfield, Wisconsin 54449, USA
Clin Med Res 2:1-3. 2004
Surveillance of above- and below-ground mosquito breeding habitats in a rural midwestern community: baseline data for larvicidal control measures against West Nile Virus vectorsTamara A Kronenwetter-Koepel
Clinical Research Center, Marshfield Clinic Research Foundation, 1000 N. Oak Avenue, Marshfield, Wisconsin 54449, USA
Clin Med Res 3:3-12. 2005....
A LightCycler TaqMan assay for detection of Borrelia burgdorferi sensu lato in clinical samplesLynn Ivacic
Molecular Diagnostics Genotyping Laboratory, Marshfield Clinic, Marshfield, Wisconsin, WI 54449, USA
Diagn Microbiol Infect Dis 57:137-43. 2007..The developed assay is robust, is highly sensitive and specific for B. burgdorferi sensu lato, and is suitable for clinical detection of the bacterium in biologic samples...
Contribution of polymorphisms in ankA, gltA, and groESL in defining genetic variants of Anaplasma phagocytophilumSanjay K Shukla
Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, 1000 North Oak Avenue, Marshfield, WI 54449, USA
J Clin Microbiol 45:2312-5. 2007..The multigene polymorphism approach holds promise for development of a genotyping scheme for this important pathogen...
Shift in Staphylococcus aureus clone linked to an infected tattooMary E Stemper
Marshfield Laboratories, Marshfield, Wisconsin 54449, USA
Emerg Infect Dis 12:1444-6. 2006..Case timeline indicated a displacement of USA400 clone by USA300 clone. The USA300 index case was associated with an infected new tattoo...
West Nile virus infection in commercial waterfowl operation, WisconsinJennifer K Meece
Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449, USA
Emerg Infect Dis 12:1451-3. 2006..Retrospective analysis of dead and live birds was conducted. WNV was detected by PCR in 84.1% of 88 dead birds; neutralizing antibodies were found in 14 of 30 randomly sampled asymptomatic or recovered birds...
Necrotizing fasciitis due to a methicillin-sensitive Staphylococcus aureus isolate harboring an enterotoxin gene clusterWilliam R Morgan
Department of Internal Medicine, Marshfield Clinic, Marshfield, Wisconsin, USA
J Clin Microbiol 45:668-71. 2007..The etiological agent was a Staphylococcus aureus isolate harboring the enterotoxin gene cluster seg, sei, sem, sen, and seo but lacked all common toxin genes, including Panton-Valentine leukocidin...
Sporadic "transitional" community-associated methicillin-resistant Staphylococcus aureus strains from health care facilities in the United StatesJennifer M Brady
Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, Marshfield, WI 54449, USA
J Clin Microbiol 45:2654-61. 2007..The potential role of PVL-carrying phages and plasmids in the success of CA-MRSA clones has been discussed...
MRSA case studiesKurt D Reed
Emerging Infectious Disease Laboratory, Marshfield Clinic Research Foundation, Marshfield, WI, USA
Methods Mol Biol 391:21-8. 2007..Significant genetic diversity exists among MRSA and sensitive strains of S. aureus. It is obvious that various combinations of virulence factors contribute to disease manifestations of infected patients...
Pulsed-field gel electrophoresis of MRSAKurt D Reed
Emerging Infectious Disease Laboratory, Marshfield Clinic Research Foundation, Marshfield, WI, USA
Methods Mol Biol 391:59-69. 2007..Banding patterns are captured by an imaging system and comparisons are made based on the Dice coefficient and the unweighted pair group method using arithmetic averages with BioNumerics software...
Clustering of multiple endemic strains of methicillin-resistant Staphylococcus aureus in a nursing home: an 8-year studyPaul J Drinka
Wisconsin Veterans Home, King 54946 0620, USA
Infect Control Hosp Epidemiol 26:215-8. 2005..05) and space (P < .05) (i.e., 2 separate statistically significant tests). Isolates from individual units were genetically related, with only the occasional unrelated isolate...
Emergence and spread of community-associated methicillin-resistant Staphylococcus aureus in rural Wisconsin, 1989 to 1999Mary E Stemper
Clinical Research Center, Marshfield Clinic Research Foundation, 1000 North Oak Ave, Marshfield, WI 54449, USA
J Clin Microbiol 42:5673-80. 2004..Two early CA-MRSA strains (WI-33 and WI-34) in Wisconsin represent progenitors of the MW2 strain, based on their almost indistinguishable genotypic characteristics...
Molecular characteristics of nosocomial and Native American community-associated methicillin-resistant Staphylococcus aureus clones from rural WisconsinSanjay K Shukla
Molecular Microbiology Laboratory, Clinical Research Center, Marshfield Clinic Research Foundation, 1000 North Oak Ave, Marshfield, WI 54449, USA
J Clin Microbiol 42:3752-7. 2004..The 19 mCGs were represented by diverse SCCmec types and three putative new variants referred to as SCCmec Ib, IIa, and IIb...
Second case of infective endocarditis caused by Gemella sanguinisSanjay K Shukla
Clinical Research Center, Marshfield Medical Research and Education Foundation, 1000 N Oak Ave, Marshfield, WI 54449, USA
WMJ 101:37-9. 2002..A subsequent transesophageal echocardiogram demonstrated vegetations on both mitral leaflets and severe mitral regurgitation by color flow imaging. A gram-positive coccus was isolated from the blood culture of this patient...
Leptotrichia amnionii sp. nov., a novel bacterium isolated from the amniotic fluid of a woman after intrauterine fetal demiseSanjay K Shukla
Clinical Research Center, Marshfield Medical Research and Education Foundation, Wisconsin 54449, USA
J Clin Microbiol 40:3346-9. 2002..The 1,493-bp 16S ribosomal DNA sequence had only 96% homology with L. sanguinegens. Several phylogenetic analyses indicated that L. amnionii is a distinct species and most closely related to L. sanguiegens...
An update on the epidemiology, pathogenesis and management of infective endocarditis with emphasis on Staphylococcus aureusTahir Tak
Department of Cardiology, Marshfield Clinic, 1000 N Oak Ave, Marshfield, WI 54449, USA
WMJ 101:24-33. 2002..In this review we will focus on the epidemiology, microbiology, and pathogenesis of S aureus IE, and also summarize the current guidelines for diagnosis, treatment, and prophylaxis of this clinical condition...
Molecular epidemiology of methicillin-resistant Staphylococcus aureus in a Veterans Administration Medical CenterPaul J Drinka
Infect Control Hosp Epidemiol 24:233-4. 2003
Rapid identification of bacteria from positive blood cultures by terminal restriction fragment length polymorphism profile analysis of the 16S rRNA geneJeffrey E Christensen
Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449, USA
J Clin Microbiol 41:3790-800. 2003..The ability to rapidly (i.e., within approximately 8 h) identify bacteria from blood cultures has potential for reducing unnecessary use of broad-spectrum antibiotics and promoting more timely prescription of appropriate antibiotics...
Importance of primer specificity for PCR detection of Anaplasma phagocytophila among Ixodes scapularis ticks from WisconsinSanjay K Shukla
J Clin Microbiol 41:4006. 2003
Is a black-pigmented Corynebacterium species an opportunistic pathogen during pregnancy? Literature review and report of 3 new casesSanjay K Shukla
Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449, USA
Clin Infect Dis 37:834-7. 2003..Similarities in these cases suggest that this newly recognized Corynebacterium species might be an opportunistic pathogen in pregnancy...
Corynebacterium nigricans sp. nov.: proposed name for a black-pigmented Corynebacterium species recovered from the human female urogenital tractSanjay K Shukla
Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449, USA
J Clin Microbiol 41:4353-8. 2003..The type strain of Corynebacterium nigricans is CN-1. It is deposited in the American Type Culture Collection (assigned strain number ATCC 700975) and in the Institute Pasteur collection (assigned strain number CIP 107346)...
Isolation of a fastidious Bergeyella species associated with cellulitis after a cat bite and a phylogenetic comparison with Bergeyella zoohelcum strainsSanjay K Shukla
Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Neillsville, Wisconsin 54449, USA
J Clin Microbiol 42:290-3. 2004..Despite sharing a close genetic relationship with other B. zoohelcum strains, this isolate was extremely fastidious in nature, raising the possibility that similar strains from cat or dog bite wound infections have been underreported...
Novel polymorphisms in mec genes and a new mec complex type in methicillin-resistant Staphylococcus aureus isolates obtained in rural WisconsinSanjay K Shukla
Molecular Microbiology Laboratory, Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449, USA
Antimicrob Agents Chemother 48:3080-5. 2004..There was also a wide range of oxacillin MICs for strains with wild-type mecA, mecI, and mecR1 sequences, suggesting that the genetic backgrounds of clinical strains are significant in determining susceptibility to methicillin...
The detection of monkeypox in humans in the Western HemisphereKurt D Reed
Department of Pathology, Marshfield Clinic, Marshfield, Wisc, USA
N Engl J Med 350:342-50. 2004..Zoonotic transmission of a bacterial or viral pathogen was suspected...
Screening for methicillin-resistant Staphylococcus aureus in a nursing homePaul J Drinka
Infect Control Hosp Epidemiol 25:95-6. 2004
Is methicillin-resistant Staphylococcus aureus more contagious than methicillin-susceptible S. aureus in a surgical intensive care unit?Paul J Drinka
Infect Control Hosp Epidemiol 25:363-4. 2004
Serologic evidence of West Nile virus infection in three wild raptor populationsWilliam E Stout
W2364 Heather Street, Oconomowoc, WI 53066, USA
Avian Dis 49:371-5. 2005..Based on breeding population densities and reproductive success over the past 15 yr, we found no apparent adverse effects of WNV infections on these wild raptor populations...
Ecologic niche modeling of Blastomyces dermatitidis in WisconsinKurt D Reed
Marshfield Clinic Research Foundation, Marshfield, Wisconsin, United States of America
PLoS ONE 3:e2034. 2008..The ecology of this pathogen is poorly understood, in part because it has been extremely difficult to identify the niche(s) it occupies based on culture isolation of the organism from environmental samples...
Bilateral prosthetic knee infection by Campylobacter fetusJohn David
Department of General Internal Medicine, Marshfield Clinic, Wisconsin 54449, USA
J Arthroplasty 20:401-5. 2005..It is important to be aware of the possibility of C fetus joint infections in high-risk patients who present with pain after total joint arthroplasty...
Coinfections acquired from ixodes ticksStephen J Swanson
Epidemic Intelligence Service Program, Office of Workforce and Career Development, Centers for Disease Control and Prevention, Atlanta, GA, USA
Clin Microbiol Rev 19:708-27. 2006..Clinicians should consider the likelihood of coinfection when pursuing laboratory testing or selecting therapy for patients with tick-borne illness...
A field evaluation of four larval mosquito control methods in urban catch basinsPatrick J Stockwell
Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, WI 54449, USA
J Am Mosq Control Assoc 22:666-71. 2006....
Rapid molecular diagnosis of lactobacillus bacteremia by terminal restriction fragment length polymorphism analysis of the 16S rRNA geneJeffrey E Christensen
Clinical Research Center, Marshfield Clinic Research Foundation, Marshfield, Wisconsin 54449, USA
Clin Med Res 2:37-45. 2004..T-RFLP profiling represents a highly reproducible and predictive source for identification of many organisms associated with bacteremia...
Identification of mosquito bloodmeal source by terminal restriction fragment length polymorphism profile analysis of the cytochrome B geneJennifer K Meece
Clinical Research Center, Marshfield Clinic Research Foundation, 1000 N Oak Avenue, Marshfield, WI 54449, USA
J Med Entomol 42:657-67. 2005..T-RFLP analysis is a highly reproducible technique and the searchable TRF database is continually being expanded to include additional species from diverse geographic areas...
Dissecting plagueKurt D Reed
Clin Med Res 4:161-2. 2006
Expression of concern--Mavoungou E. Interactions between natural killer cells, cortisol and prolactin in malaria during pregnancy. Clin Med Res 2006;4:33-41Kurt D Reed
Clin Med Res 6:2. 2008
